CN105230577B - The continuous cultural method of Brachionus calyciflorus - Google Patents

The continuous cultural method of Brachionus calyciflorus Download PDF

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CN105230577B
CN105230577B CN201510844350.0A CN201510844350A CN105230577B CN 105230577 B CN105230577 B CN 105230577B CN 201510844350 A CN201510844350 A CN 201510844350A CN 105230577 B CN105230577 B CN 105230577B
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water
wheel animalcule
water body
individual
cultivation
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CN105230577A (en
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陈旭
张六六
夏森玉
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JIANGSU LUCKER BIO-ENGINEERING Co Ltd
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JIANGSU LUCKER BIO-ENGINEERING Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

The present invention relates to a kind of continuous cultural method of Brachionus calyciflorus, it is characterized in that, comprise the following steps：（1）Support water：Cement pit is made in room is cultivated as culture pond, cultivates in the previous circumferential culture pond of wheel animalcule and is put into water, to breeding water body sterilization and static one week or so；Breeding water body is conserved；（2）The water temperature of breeding water body is controlled at 30 ~ 35 DEG C, inoculating rotifer after maintaining 6 ~ 8 hours；（3）Control water：By adding the ammonia-nitrogen content in microbiological garbage treatment agent regulation breeding water body in incubation, ensure that wheel animalcule continuously cultivates the stability of water body environment；（4）Harvesting：After culture 60 ~ 72 hours, filter cultivation water and collect wheel animalcule, the cultivation water after filtering is reentered into culture pond and continues to cultivate wheel animalcule as stated above.The present invention can be achieved to be changed without cultivation water within 30 ~ 40 days during cultivation wheel animalcule, and the average wheel animalcule yield that obtains daily is up to 70 ~ 100/mL water, and wheel animalcule individual nutrition is balanced comprehensively, improves the reproductive survival rate such as fish, shrimp, crab in aquaculture.

Description

The continuous cultural method of Brachionus calyciflorus
Technical field
The present invention relates to a kind of continuous cultural method of Brachionus calyciflorus, belong to technical field of aquaculture.
Background technology
Wheel animalcule（Rotifer）It is a kind of small（0.04~4.0 mm）Aquatic multicellular animals, it is each to be distributed widely in the world It is the important component of planktonic organism in aquatic ecosystem in the fresh water on ground, seawater and Inland Saline Water.It is wherein most economical Meaning, the development to world's fish artificial breeding industry has the species of significant impact, surely belongs to Brachionus calyciflorus.Calyx spends arm tail Wheel animalcule belongs to light aqueous biological, and it has a variety of distinctive nutriments, the children that can be used as after the hatching such as all kinds of fishes, shrimp, crab The top quality open-mouthed bait of seedling.
The high benefit cultural techonology of Brachionus calyciflorus is that the extensive artificial breeding of fish Shrimp waste batch production is achieved Basis.However, requirement of the Brachionus calyciflorus for water body environment and bait is very strict, continuous culture large-scale at present It is difficult to accomplish to be, most Brachionus calyciflorus culture technique is batch culture at this stage, not only the horizontal relatively low, culture of culture Cycle is long, needs a large amount of manpower and materials to support, while the product quality cultivated differs, and cannot effectively ensure, serious to hinder Brachionus calyciflorus is used for the prospect of aquaculture bait.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, there is provided a kind of continuous culture of Brachionus calyciflorus Method, efficiently, it is easy, reliable, suitable for large-scale application.
According to technical scheme provided by the invention, the continuous cultural method of the Brachionus calyciflorus, it is characterized in that, including Following steps：
（1）Support water：
Cement pit is made in room is cultivated as culture pond, cultivates in the previous circumferential culture pond of wheel animalcule and is put into water, with raw stone Ash uniformly sowed full pond, to breeding water body sterilization and static one week or so；Into breeding water body by being added in every liter of breeding water body Active chlorella 1 × 106~4×106Individual, lactobacillus 1 × 106~2×106Individual, bacillus subtilis 1 × 106~2×106Individual, fourth Sour clostridium 1 × 105~2×105The g of chick excrement 1 ~ 2 after individual and fermentation, and water pH value is adjusted to 6.5 ~ 7.5, aeration 2 ~ 3 is small When；
（2）Inoculation：
The water temperature of breeding water body is controlled to press 200 ~ 300 wheel animalcule/m after maintaining 6 ~ 8 hours at 30 ~ 35 DEG C2Breeding water body Measure inoculating rotifer；
（3）Control water：
When cultivation water pH value is more than or equal to 8.0, added after breeding water body is aerated 15 ~ 30 minutes by every liter of cultivation water The g of cattle manure 2 ~ 4, saccharomycete 2 × 10 after high-temperature anaerobic fermentation5~4×105Individual, bacillus subtilis 1 × 105~2×105It is individual The microbiological garbage treatment agent of composition；
When pH is more than or equal to 8.5, by the son after every liter of cultivation water addition fermentation after breeding water body is aerated 15 ~ 30 minutes The g of chicken manure 2 ~ 4, saccharomycete 2 × 105~4×105Individual, clostridium butyricum 1 × 105~2×105The microbiological garbage treatment agent of individual composition；
（4）Harvesting：
After culture 60 ~ 72 hours, with the filter screen filtration cultivation waters of 250 mesh and wheel animalcule is collected, by the cultivation water after filtering It is reentered into culture pond and continues to cultivate wheel animalcule as stated above.
In a detailed embodiment, the culture pond uses area as 100 m2, deep 0.5 m cement pit.
In a detailed embodiment, the step（1）Chick liquid dung after the fermentation of middle addition is divided into 30% or so.
In a detailed embodiment, the step（3）Cattle manure after the high-temperature anaerobic fermentation of middle addition it is aqueous Measure as 15% or so.
In a detailed embodiment, the step（3）The water content of chick excrement after the fermentation of middle addition is 30% left It is right.
In a detailed embodiment, ensure to occur without dry pond phenomenon in the recovery process of the wheel animalcule.
The continuous cultural method of Brachionus calyciflorus of the present invention, it can be achieved during cultivation wheel animalcule 30 ~ 40 days not more Cultivation water is changed, the average wheel animalcule yield that obtains daily is up to 70 ~ 100/mL water, and wheel animalcule individual nutrition is balanced comprehensively, improves water The reproductive survival rate such as fish, shrimp, crab reaches more than 95% in production cultivation.It is contemplated that with less space, lower labour into Sheet and shorter production time, continuously cultivated in wheel animalcule produces, ensure the supply of aquatic livestock opening living bait, have Efficiently, the features such as easy, economic, reliable, suitable for large-scale application.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1：A kind of continuous cultural method of Brachionus calyciflorus, comprises the following steps：
（1）Support water：
Water body environment is the most critical factor of wheel animalcule growth, therefore it is the base for cultivating freshwater rotifer that breeding water body, which is conserved, Plinth.Mainly by imitating living environment of the wheel animalcule in nature, rational allocation adds microorganism sealing compound to complete for maintenance.
Area is used as 100 m2, deep 0.5 m cement pit is as culture pond, and with the preferable greenhouse of sealing or simply House is as culture room.Culture wheel animalcule the last week is first put into the deep water of 0.3 m, full pond is uniformly sowed with quick lime, to cultivation water Body sterilize and static one week or so.According to the testing result for disinfecting rear breeding water body, into water body by every liter of cultivation water Add active chlorella 1 × 106Individual, lactobacillus 1 × 106Individual, bacillus subtilis 1 × 106Individual, clostridium butyricum 1 × 105It is individual and Chick excrement after fermentation（Moisture 30%）1 ~ 2 g, and water pH value is adjusted to 6.5, it is aerated 2 hours, aeration described herein refers to Daylight lower day exposure 2 hours.
（2）Inoculation：
The water temperature of breeding water body is controlled to press 200 wheel animalcule/m after maintaining 8 hours at 30 DEG C2The amount inoculation wheel of breeding water body Worm；
（3）Control water：
When wheel animalcule density reaches 10/mL breeding water bodies, because wheel animalcule can drain many excrement, meeting in growth course The higher generation nitrite of ammonia nitrogen in water body is caused, detrimental effects can be produced to wheel animalcule growth.Although changing water can temporarily solve, But change again water can cause in the wheel animalcule short time can not flourish and ammonia nitrogen may proceed to raise after 12h.So must Must timely processing wheel fecula stool and ensureing do not influence to carry out on the basis of wheel animalcule growth.The present invention is main through the following two-step Method absorbs ammonia nitrogen degradation nitrite and decomposes excrement, but also can strengthen wheel animalcule constitution and nutritional ingredient reaches and do not change water for a long time Purpose.
The first step：When cultivation water pH value is more than or equal to 8.0, every liter of cultivation water is pressed after breeding water body is aerated 15 minutes Add the g of cattle manure 2, saccharomycete 2 × 10 after high-temperature anaerobic fermentation5Individual, bacillus subtilis 1 × 105The microorganism of individual composition Inorganic agent；Second step：When pH is more than or equal to 8.5, after being fermented after breeding water body is aerated 15 minutes by every liter of cultivation water addition The g of chick excrement 2, saccharomycete 2 × 105, clostridium butyricum 1 × 105The microbiological garbage treatment agent of individual composition.
（4）Harvesting：
With the filter screen filtration cultivation water of 250 mesh and wheel animalcule was collected every 72 hours, the cultivation water after filtering is relay Enter and continue to cultivate wheel animalcule as stated above in culture pond, complete the continuous culture of 30 days altogether, collect the wheel animalcule of 10 batches, it is average Daily wheel animalcule yield is 75/mL breeding water bodies；Ensure to occur without dry pond phenomenon during collection.
Embodiment 2：A kind of continuous cultural method of Brachionus calyciflorus, comprises the following steps：
（1）Support water：
Area is used as 100 m2, deep 0.5 m cement pit is as culture pond, and with the preferable greenhouse of sealing or simply House is as culture room.Culture wheel animalcule the last week is first put into the deep water of 0.3 m, full pond is uniformly sowed with quick lime, to cultivation water Body sterilize and static one week or so.According to the testing result for disinfecting rear breeding water body, into water body by every liter of cultivation water Add active chlorella 4 × 106Individual, lactobacillus 2 × 106Individual, bacillus subtilis 2 × 106Individual, clostridium butyricum 2 × 105It is individual and Chick excrement after fermentation（Moisture 30%）2 g, and water pH value is adjusted to 7.5, it is aerated 3 hours, aeration described herein referred in day Light lower day exposure 3 hours.
（2）Inoculation：
The water temperature of breeding water body is controlled to press 300 wheel animalcule/m after maintaining 6 hours at 35 DEG C2The amount inoculation wheel of breeding water body Worm；
（3）Control water：
It is high by every liter of cultivation water addition after breeding water body is aerated 30 minutes when cultivation water pH value is more than or equal to 8.0 The g of cattle manure 4, saccharomycete 4 × 10 after warm anaerobic fermentation5Individual, bacillus subtilis 2 × 105The microbiological garbage treatment agent of individual composition；
When pH is more than or equal to 8.5, by the chick after every liter of cultivation water addition fermentation after breeding water body is aerated 30 minutes The g of excrement 4, saccharomycete 4 × 105Individual, clostridium butyricum 2 × 105The microbiological garbage treatment agent of individual composition.
（4）Harvesting：
With the filter screen filtration cultivation water of 250 mesh and wheel animalcule was collected every 68 hours, the cultivation water after filtering is relay Enter and continue to cultivate wheel animalcule as stated above in culture pond, complete the continuous culture of 34 days altogether, collect the wheel animalcule of 12 batches, it is average Daily wheel animalcule yield is 87/mL breeding water bodies；Ensure to occur without dry pond phenomenon during collection.
Embodiment 3：A kind of continuous cultural method of Brachionus calyciflorus, comprises the following steps：
（1）Support water：
Area is used as 100 m2, deep 0.5 m cement pit is as culture pond, and with the preferable greenhouse of sealing or simply House is as culture room.Culture wheel animalcule the last week is first put into the deep water of 0.3 m, full pond is uniformly sowed with quick lime, to cultivation water Body sterilize and static one week or so.According to the testing result for disinfecting rear breeding water body, into water body by every liter of cultivation water Add active chlorella 2 × 106Individual, lactobacillus 1.5 × 106Individual, bacillus subtilis 1.5 × 106Individual, clostridium butyricum 1.5 × 105Chick excrement after individual and fermentation（Moisture 30%）1.5g, and water pH value is adjusted to 7, it is aerated 2.5 hours, aeration described herein Refer to day exposure 2.5 hours in the sunlight.
（2）Inoculation：
The water temperature of breeding water body is controlled to press 250 wheel animalcule/m after maintaining 7 hours at 32 DEG C2The amount inoculation wheel of breeding water body Worm；
（3）Control water：
It is high by every liter of cultivation water addition after breeding water body is aerated 20 minutes when cultivation water pH value is more than or equal to 8.0 The g of cattle manure 3, saccharomycete 3 × 10 after warm anaerobic fermentation5Individual, bacillus subtilis 1.5 × 105The microbiological treatment of individual composition Agent；
When pH is more than or equal to 8.5, by the chick after every liter of cultivation water addition fermentation after breeding water body is aerated 20 minutes The g of excrement 3, saccharomycete 3 × 105Individual, clostridium butyricum 1.5 × 105The microbiological garbage treatment agent of individual composition.
（4）Harvesting：
With the filter screen filtration cultivation water of 250 mesh and wheel animalcule was collected every 60 hours, the cultivation water after filtering is relay Enter and continue to cultivate wheel animalcule as stated above in culture pond, complete the continuous culture of 40 days altogether, collect the wheel animalcule of 16 batches, it is average Daily wheel animalcule yield is 104/mL breeding water bodies；Ensure to occur without dry pond phenomenon during collection.
The sealing compound of water body is made up of various active microorganism and microbial fermentation product before present invention culture wheel animalcule, and Corresponding foodstuff is provided for wheel animalcule.In incubation, breeding water body environment is adjusted using microorganism means, control is supported Grow the content of ammonia nitrogen in water body.After wheel animalcule reaches predetermined yield and collects, the cultivation water after filtering wheel animalcule is reentered into training Pond is supported, realizes continuous culture wheel animalcule.

Claims (1)

1. a kind of continuous cultural method of Brachionus calyciflorus, it is characterized in that, comprise the following steps：
（1）Support water：
In room is cultivated make cement pit be used as culture pond, culture the previous circumferential culture pond of wheel animalcule in be put into water, it is equal with quick lime It is even to sow full pond, breeding water body is sterilized and stands one week or so；Into breeding water body by adding activity in every liter of breeding water body Chlorella 1 × 106~4×106Individual, lactobacillus 1 × 106~2×106Individual, bacillus subtilis 1 × 106~2×106Individual, butyric acid shuttle Bacterium 1 × 105~2×105The g of chick excrement 1 ~ 2 after individual and fermentation, and water pH value is adjusted to 6.5 ~ 7.5, it is aerated 2 ~ 3 hours；Institute State culture pond and use area as 100 m2, deep 0.5 m cement pit；Chick liquid dung after the fermentation of addition is divided into 30% or so；
（2）Inoculation：
The water temperature of breeding water body is controlled to press 200 ~ 300 wheel animalcule/m after maintaining 6 ~ 8 hours at 30 ~ 35 DEG C2The amount of breeding water body connects Kind wheel animalcule；
（3）Control water：
When cultivation water pH value is more than or equal to 8.0, high temperature is added by every liter of cultivation water after breeding water body is aerated 15 ~ 30 minutes The g of cattle manure 2 ~ 4, saccharomycete 2 × 10 after anaerobic fermentation5~4×105Individual, bacillus subtilis 1 × 105~2×105Individual composition Microbiological garbage treatment agent；
When pH is more than or equal to 8.5, by the chick excrement after every liter of cultivation water addition fermentation after breeding water body is aerated 15 ~ 30 minutes 2 ~ 4 g, saccharomycete 2 × 105~4×105Individual, clostridium butyricum 1 × 105~2×105The microbiological garbage treatment agent of individual composition；
The water content of cattle manure after the high-temperature anaerobic fermentation of addition is 15% or so；The step（3）After the fermentation of middle addition The water content of chick excrement is 30% or so；
（4）Harvesting：
After culture 60 ~ 72 hours, with the filter screen filtration cultivation waters of 250 mesh and wheel animalcule is collected, by the cultivation water after filtering again It is put into culture pond and continues to cultivate wheel animalcule as stated above；Ensure to occur without dry pond phenomenon in the recovery process of the wheel animalcule.
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CN104082248A (en) * 2014-06-23 2014-10-08 浙江省淡水水产研究所 Method for small-scale and simple culturing of freshwater rotifers
CN104255671A (en) * 2014-09-26 2015-01-07 盐城工学院 Branchionus plicatilis artificial water circulation high-yield breeding system and method
CN104969908A (en) * 2014-04-14 2015-10-14 东营大振生物科技有限公司 A rotifer high-density culture method

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
CN102511432A (en) * 2012-01-13 2012-06-27 广东海洋大学 Method for cultivating Brachionus plicatilis
CN104969908A (en) * 2014-04-14 2015-10-14 东营大振生物科技有限公司 A rotifer high-density culture method
CN104082248A (en) * 2014-06-23 2014-10-08 浙江省淡水水产研究所 Method for small-scale and simple culturing of freshwater rotifers
CN104255671A (en) * 2014-09-26 2015-01-07 盐城工学院 Branchionus plicatilis artificial water circulation high-yield breeding system and method

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