CN108587984B - Selenium-rich spirulina culture, and culture method and application thereof - Google Patents
Selenium-rich spirulina culture, and culture method and application thereof Download PDFInfo
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- 239000011669 selenium Substances 0.000 title claims abstract description 86
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 85
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 84
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 62
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 62
- 229940082787 spirulina Drugs 0.000 title claims abstract description 62
- 238000012136 culture method Methods 0.000 title description 4
- 238000012258 culturing Methods 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 229940091258 selenium supplement Drugs 0.000 claims description 77
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical group [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 12
- 235000019345 sodium thiosulphate Nutrition 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 8
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical group [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 6
- 239000011781 sodium selenite Substances 0.000 claims description 6
- 229960001471 sodium selenite Drugs 0.000 claims description 6
- 235000015921 sodium selenite Nutrition 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 239000003223 protective agent Substances 0.000 claims description 5
- 239000003674 animal food additive Substances 0.000 claims description 3
- 239000002778 food additive Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 10
- 238000007792 addition Methods 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 238000005457 optimization Methods 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
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- 230000008020 evaporation Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003002 pH adjusting agent Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 235000016491 selenocysteine Nutrition 0.000 description 1
- 229940055619 selenocysteine Drugs 0.000 description 1
- 229960002718 selenomethionine Drugs 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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Abstract
The invention relates to a method for culturing selenium-enriched spirulina, which comprises the following steps: s1: culturing spirulina to logarithmic phase by using a whole culture medium, and culturing spirulina culture in logarithmic phase; s2: adding a selenium-containing reagent into the logarithmic phase spirulina culture to induce the spirulina to be rich in selenium, and culturing for 3-5 days to obtain the selenium-rich spirulina culture. The spirulina cultured by the method can keep higher growth speed, thereby improving the yield, and on the premise of ensuring the yield, the selenium content in the spirulina is improved to 800 mug/g dry weight, wherein the organic selenium accounts for more than 80 percent.
Description
Technical Field
The invention relates to the field of microalgae culture and application of high-added-value microalgae, in particular to a method for culturing selenium-enriched spirulina, a selenium-enriched spirulina culture obtained by using the method and application of the selenium-enriched spirulina culture.
Background
Selenium (Se) is a trace element necessary for animals and human beings, and can be used for preventing and treating various diseases by supplementing selenium. However, inorganic selenium such as selenate is not easily absorbed by the human or animal body. Therefore, there is a need to obtain organic selenium from food. Some plants can enrich selenium in the environment, synthesize organic selenium in vivo, and supplement selenium in vivo by making such plants into additives to be added into food or medicine, or directly eating edible parts of such plants.
Several patents and technical literature have disclosed methods for culturing selenium-rich plants, such as selenium-rich tea, peanuts, rice, melons and fruits. However, the growth cycle of terrestrial higher plants is long, the growth conditions are not easy to control, the final results are also uneven, and the selenium content of the plants is not high.
Microalgae are a group of lower plants that are unicellular or multicellular. Many types of microalgae are rich in nutritional ingredients such as protein, oil and fat, polysaccharide and the like, and other types of microalgae are rich in physiological active ingredients such as unsaturated fatty acid, carotenoid, vitamin and the like. The dried powder of algae such as Spirulina and Chlorella can be used as food or feed additive for remarkably improving metabolism and disease resistance of human or animal. In addition, the growth cycle of the microalgae is short, the culture conditions are easy to control, and the microalgae is very convenient to process at the later stage due to the microorganisms. However, spirulina or other microalgae have poor growth conditions in high selenium solutions because during the internalization of selenium, the synthesized selenomethionine and selenocysteine replace methionine and cysteine in peptide chain synthesis, thereby producing selenium toxicity. Therefore, there is no spirulina with high selenium content and its products in the market, and a new microalgae culture method is needed to culture selenium-rich microalgae.
Disclosure of Invention
In order to solve the above problems, the present invention provides a method for culturing selenium-enriched spirulina, which is characterized by comprising the following steps:
s1: culturing spirulina to logarithmic phase by using a whole culture medium, and culturing spirulina culture in logarithmic phase;
s2: adding a selenium-containing reagent into the logarithmic phase spirulina culture to induce the spirulina to be rich in selenium, and culturing for 3-5 days to obtain the selenium-rich spirulina culture.
In one embodiment, the selenium-containing reagent of S2 is sodium selenite solution
In one embodiment, S2 includes the following steps:
s21: adding a protectant to the log phase spirulina culture;
s22: placing the log-phase spirulina culture added with the protective agent under the condition of high-light intensity illumination;
s23: adding the selenium-containing reagent into the selenium-enriched spirulina culture for multiple times every day, and culturing for 5-8 days to obtain the selenium-enriched spirulina culture.
In a preferred embodiment, the log phase spirulina culture is treated for 20-40 minutes before and after each addition of the selenium containing agent in S23.
In a preferred embodiment, the selenium containing reagent is added 3-5 times per day in S23 in an amount of 100-150 mg/L selenium based on the volume of the logarithmic phase Spirulina culture.
In a preferred embodiment, the protecting agent in S21 is sodium thiosulfate, and is added in an amount of 500-750 mg/L based on the volume of the logarithmic phase spirulina culture.
In a preferred embodiment, the high light intensity condition is 350-500. mu.E.m in S22-2·s-1。
In a specific embodiment, the whole medium described in S1 is a Zarrouk medium.
The invention also provides a selenium-rich spirulina culture which is obtained by the culture method.
The invention also provides application of the selenium-enriched spirulina culture in preparation of food or feed additives.
The spirulina cultured by the method can keep higher growth speed, thereby improving the yield, and on the premise of ensuring the yield, the selenium content in the spirulina is improved to 800 mug/g dry weight, wherein the organic selenium accounts for more than 80 percent.
Drawings
FIG. 1 is a graph showing the growth curves of spirulina in the process of induced culture of selenium-enriched spirulina in experimental group 1, experimental group 2 and control group;
FIG. 2 is a bar graph of the yield of algal powder and the total selenium content obtained by culturing Spirulina under different light intensities;
FIG. 3 is a bar graph of the yield of algal powder and the total selenium content obtained by culturing Spirulina at different sodium selenite additions;
FIG. 4 is a bar graph of algal meal yield and total selenium content obtained by culturing Spirulina at different sodium thiosulfate additions.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
1. Culturing Spirulina to logarithmic phase
Spirulina was cultured using Zarrouk medium, the medium composition of which is shown in Table 1.
TABLE 1 composition of Zarrouk Medium
| Components | Concentration (g/L) | Components | Concentration (g/L) |
| NaHCO3 | 16.8 | K2HPO4 | 0.50 |
| NaNO3 | 2.50 | FeSO4·7H2O | 0.01 |
| NaCl | 1.00 | Na2-EDTA | 0.08 |
| K2SO4 | 1.00 | CaCl2 | 0.08 |
| MgSO4·7H2O | 0.20 | Solution A5 | 2 mL/L |
TABLE 2A 5 composition of the solution
| Components | Concentration (g/L) |
| H3BO3 | 2.86 |
| (NH4)6Mo17O24 | 0.02 |
| MnCl2·4H2O | 1.80 |
| CuSO4·5H2O | 0.08 |
| ZnSO4·7H2O | 0.22 |
Inoculating spirulina seed to a culture medium containing 150 wt.% of spirulinaInoculation amount of OD in 250 mL triangular flask of mL culture medium560= 0.10. Placing the triangular flask in 30-35 deg.C constant temperature shaking table for culturing with illumination intensity of 25-40 μ E.m-2·s-1. Distilled water was supplemented according to the amount of evaporation, and a pH adjuster was appropriately added to maintain the pH in the range of 9.5 to 10.5. The culture time is 5-8 days, and OD of culture solution560Reaching 1-1.5, in logarithmic growth phase.
2. Selenium enrichment induction
Adding sodium thiosulfate into spirulina culture solution in logarithmic growth phase to make its concentration be 500 mg/L, and standing at 350 μ E · m-2·s-1The culture is carried out under the light intensity of (1), sodium selenite is added into the culture solution for 3 times every day, and the addition amount of each time is 100 mg/L selenium equivalent. Distilled water was supplemented according to the amount of evaporation, and a pH adjuster was appropriately added to maintain the pH in the range of 9.5 to 10.5. The culture time was 5 days. As experimental group 1.
So that the illumination intensity is 25-40 muE.m all the time-2·s-1Next, Zarrouk medium was cultured as a control group.
After 5 days of culture, each group of spirulina cells was collected by centrifugation and washed with pure water several times to remove the medium components. Weighing and detecting the selenium content. The results showed that the control group obtained 3.85 g/L of dry powder and contained substantially no selenium, the experimental group obtained 1.83 + -0.35 g/L of dry powder, the total selenium content was 632 + -15.82 μ g/g dry weight, and the organic selenium accounted for about 85% of the total selenium. In general, in experimental group 1, it was shown that the high-Se medium and high illumination had certain effect on the growth of spirulina, but still had a healthy growth and selenium-rich effect. Therefore, the protective agent sodium thiosulfate can help spirulina cells to resist selenium toxicity on one hand, and the reducibility of the sodium thiosulfate can resist oxidative damage caused by high light.
3. Adding dark treatment
On the basis of the culture conditions of the experimental group 1, the improvement is carried out, and dark treatment is carried out for 20-40 min before and after each selenium addition, so that the experimental group 2 is obtained. As shown in FIG. 1, unexpectedly, the growth state of the microalgae in the experimental group 2 is much better than that in the experimental group 1, and the yield of the spirulina is greatly increased to 3.28 +/-0.26 g/L. The total selenium content showed that the total selenium content of test group 2 was 852 + -26.37 μ g/g dry weight, and the organic selenium accounted for about 83% of the total selenium. Thus, dark treatment helps spirulina cells to resist selenium toxicity and promotes the spirulina cells to absorb selenium.
4. Condition optimization
Based on the experimental group 2, the light intensity, selenium concentration and sodium thiosulfate were optimized in sequence. And (4) carrying out next condition optimization by using the optimal conditions of the previous step experiment in each experiment.
4.1 optimization of light intensity
Based on the experimental group 2, only spirulina was cultured at 50, 100, 200, 300, 350, 400, 450, 500, 550 μ E · m-2·s-1The light intensity of the culture medium is measured, and the optimal light intensity is explored. The results are shown in FIG. 2, when the intensity is 350. mu.E.m-2·s-1In the above, the total selenium content is higher, reaching 800-. However, as the light intensity increased, the dry weight of the harvested algae decreased gradually, when the light intensity reached 550. mu.E.m-2·s-1At the same time, the harvested algae powder is only 1.22 +/-0.37 g/L. Thus, a suitable light intensity is 350--2·s-1. In the following experiments, 450 μ E · m was chosen-2·s-1Further optimization is performed as the culture light intensity.
4.2 optimization of selenium addition
Under the conditions defined above, the amount of selenium added was optimized by adding 50, 75, 100, 125, 150 mg/L selenium equivalents of sodium selenite solution each time. As shown in FIG. 3, the total selenium content of the algae meal was higher when the selenium concentration was above 100 mg/L, but at the same time, the content of the harvested algae meal was also decreased, and it was found that the increase in selenium concentration was toxic to algae. In the next experiment, the addition of 100 mg/L selenium equivalent of sodium selenite is selected to optimize the addition of sodium thiosulfate.
4.3 optimization of the amount of sodium thiosulfate added
Under the conditions determined above, the amount of sodium thiosulfate added was optimized to add 500, 600, 700, 750 mg/L sodium thiosulfate solution, respectively. The results are shown in fig. 4, where the amount of harvested dry algal powder gradually increased with increasing sodium thiosulfate concentration, while the total selenium content was not affected.
In the optimization process, the content of the organic selenium is always maintained to be more than 80 percent of the total selenium content.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (4)
1. A method for culturing selenium-enriched spirulina is characterized by comprising the following steps:
s1: culturing spirulina to logarithmic phase by using a whole culture medium, and culturing spirulina culture in logarithmic phase;
s2: adding a selenium-containing reagent into the logarithmic phase spirulina culture to induce the spirulina to be selenium-rich, and culturing for 5-8 days to obtain a selenium-rich spirulina culture;
s2 includes the steps of:
s21: adding a protectant to the log phase spirulina culture;
s22: placing the logarithmic phase spirulina culture added with the protective agent under the high-light-intensity condition of 250-450 MuE.m-2·s-1；
S23: adding the selenium-containing reagent 3-5 times a day, wherein the adding amount of selenium in each time is 100-150 mg/L of selenium according to the volume of the logarithmic phase spirulina culture;
in S21, the protective agent is sodium thiosulfate, and the addition amount is 500-750 mg/L in terms of the volume of the logarithmic phase spirulina culture; in S23, performing dark treatment on the logarithmic phase spirulina culture for 20-40 minutes before and after each addition of the selenium-containing reagent; the selenium-containing reagent is sodium selenite solution.
2. The method according to claim 1, wherein the whole medium described in S1 is Zarrouk medium.
3. A selenium-enriched spirulina culture obtained by the method of claim 1 or 2.
4. Use of a selenium enriched spirulina culture of claim 3 in the preparation of a food or feed additive.
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