CN106635913A - Method for controlling accumulation of spirulina nutrient elements - Google Patents
Method for controlling accumulation of spirulina nutrient elements Download PDFInfo
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Abstract
The invention provides a method for controlling accumulation of spirulina nutrient elements. The method comprises the steps that 1, a culture medium is inoculated with spirulina, and the spirulina is cultured for 3 days under the conditions that the temperature ranges from 20 DEG C to 40 DEG C, the luminous intensity ranges from 4 klx to 12 klx, and the pH is 8.5-10.5; 2, the spirulina obtained in the step 1 continues to be cultured under the conditions that the temperature ranges from 15 DEG C to 35 DEG C, the luminous intensity ranges from 2 klx to 20 klx, and the pH is 8.5-11, calcium, iron, zinc and selenium elements are added into an outer source of the culture medium, culturing is conducted for 3-7 days, and the spirulina with the high nutrient element content is obtained. According to the method, the contents of the calcium, iron, zinc and selenium elements in the spirulina are significantly increased, the nutrient elements are combined with organic matter in cells, irritation to the gastrointestinal tract is reduced, and the human body absorption and utilization rate is increased. The calcium content, the iron content and the zinc content in the cells of the spirulina cultured through the method are increased by 97%, 175% and 87% respectively compared with those in cells of spirulina cultured through a traditional breeding method, and the organification degrees of calcium, iron, zinc and selenium are 84.17%, 89.98%, 58.28% and 70.54% sequentially.
Description
Technical field
The present invention relates to biological cell post-directed training and extractive technique field, specifically, are related to a kind of regulation and control spirulina
The method of Nutrient Accumulation.
Background technology
In the last few years, the supplement of nutrient was increasingly paid close attention to by people.According to 1992 national nutrition survey number
According to display, the calcium intake of compatriots only has 20%-50%, and the scarce zinc rate of children is up to 60%, and Chinese hypoferric anemia rate is average
For 20% or so, about 2/3 area lacks selenium.The conventional method of these nutrient deficiencies is solved, severe one passes through drug therapy,
The lighter is by meal supplement, and food is supplemented and is generally unattainable needed by human body, and medicine mostly is inorganic constituents supplement, such as chlorination
Calcium, zinc sulfate etc., often absorption rate is low for human body.For this present situation, using spirulina cells Nutrient Absorption and richness
The characteristic of collection, as carrier, promotes its Nutrient Accumulation, and inorganic ions organises in the cell, improves human body
Absorptivity and utilization rate, are reached for the purpose of human body extra-nutrition element.
At present, the method for improving algae nutrient mainly reaches unit by increasing external source nutrient solution intermediate ion concentration
The purpose of element accumulation.However, both at home and abroad all multiple enterprises in its breeding process, are related to region, season in cultivation exploitation spirulina
Impact of the change of section, temperature difference per day etc. to algal grown and nutritious substances accumulation is very big, but has no growing environment to spirulina
The report of Nutrient Accumulation.Inventor studies through for many years, as a result shows envirment factor (temperature, environment, pH etc.) not only shadow
The growth of spirulina is rung, and affects its physiological metabolism, the function such as material synthesis also has deep to its mineral matter nutritional accumulation
Impact.Effective Regulation nutrient is accumulated for nutrient organises in spirulina cells, the supplement of human nutrition element,
Widen spirulina range of application to be significant.
The content of the invention
It is an object of the invention to provide a kind of method for improving nutritious spirulina element (calcium, iron, zinc, selenium) content.
In order to realize the object of the invention, the method for present invention regulation and control nutritious spirulina element accumulation is comprised the following steps：
1) spirulina is inoculated in culture medium, in temperature 20-40 DEG C, illumination 4-12klx, the condition of pH value 8.5-10.5
Lower culture 3 days；
2) by step 1) in spirulina continue in temperature 15-35 DEG C, illumination 2-20klx, the condition of pH value 8.5-11.0
Lower culture, respectively by adding calcium, iron, zinc, selenium element to external source in culture medium, cultivates 3-7 days, obtains the spiral shell of high nutrition element
Rotation algae.
Aforesaid method, step 2) it is specific as follows：By step 1) in spirulina continue to cultivate, adjust temperature to 15-25
DEG C, illumination control is controlled in 8.5-9.0 in 11-20klx, pH, and external source CaCO is added in culture medium3To final concentration 0.4-
0.8g/L, incubation time is 3-7 days, obtains the spirulina rich in calcium.Preferably, by step 1) in spirulina continue cultivate,
Temperature adjustment to 20 DEG C, illumination control is controlled 8.5 in 12klx, pH, and external source CaCO is added in culture medium3To final concentration
0.6g/L, cultivates 7 days, obtains the spirulina rich in calcium.
Aforesaid method, step 2) it is specific as follows：By step 1) in spirulina continue cultivate, temperature adjustment to 25-35
DEG C, illumination control is controlled in 10-11.0 in 2-6klx, pH, and external source FeSO is added in culture medium4·7H2O to final concentration 30-
60mg/L, cultivates 3-7 days, obtains the spirulina rich in iron.Preferably, by step 1) in spirulina continue cultivate, temperature adjust
Save to 30 DEG C, illumination control is controlled 10.5 in 4klx, pH, external source iron FeSO47H is added in culture medium2O is to final concentration
40mg/L, cultivates 3 days, obtains the spirulina rich in iron.
Aforesaid method, step 2) it is specific as follows：By step 1) in spirulina continue cultivate, temperature adjustment to 25-35
DEG C, illumination control is controlled in 8.5-9.5 in 6-11klx, pH, and external source ZnSO is added in culture medium4·7H2O to final concentration 30-
70mg/L, incubation time is 3-7 days, obtains the spirulina rich in zinc.Preferably, by step 1) in spirulina continue cultivate,
Temperature adjustment to 30 DEG C, illumination control is controlled 8.5 in 8klx, pH, and external source zinc ZnSO is added in culture medium4·7H2O is to end
Concentration 40mg/L, cultivates 3 days, obtains the spirulina rich in zinc.
Aforesaid method, step 2) it is specific as follows：By step 1) in spirulina continue cultivate, temperature adjustment to 28-35
DEG C, illumination control is controlled in 9.0-10.0 in more than 11klx, pH, and external source selenium Na is added in culture medium2SeO3To final concentration 80-
120mg/L, incubation time is 3-7 days, obtains the spirulina rich in selenium.Preferably, by step 1) in spirulina continue cultivate,
Temperature adjustment to 30 DEG C, illumination control is controlled 9.5 in 12klx, pH, and external source selenium Na is added in culture medium2SeO3To final concentration
100mg/L, cultivates 5 days, obtains the spirulina rich in selenium.
Preferably, step 1) it is specific as follows：Spirulina is inoculated in culture medium, at 30 DEG C, 12klx, the bar of pH value 9.5
Cultivate 3 days under part.
In a specific embodiment of the present invention, the method for regulating and controlling nutritious spirulina element accumulation is specific as follows：
1) spirulina is inoculated in culture medium, at 30 DEG C, 12klx is cultivated 3 days under conditions of pH value 9.5；
2) by step 1) in spirulina continue to cultivate, temperature adjustment to 20 DEG C, in 12klx, pH controls exist for illumination control
8.5, external source CaCO is added in culture medium3To final concentration 0.6g/L, cultivate 7 days, obtain the spirulina rich in calcium, calcium organises
Degree reaches 84.17%；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, in 4klx, pH controls exist for illumination control
10.5, external source iron FeSO47H is added in culture medium2O to final concentration 40mg/L, cultivates 3 days, obtains the spiral rich in iron
Algae, ferric organic degree reaches 89.98%；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, in 8klx, pH is controlled 8.5 for illumination control,
Add external source zinc ZnSO in culture medium4·7H2O to final concentration 40mg/L, cultivates 3 days, obtains the spirulina rich in zinc, and zinc has
Machine degree reaches 58.28%；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, in 12klx, pH controls exist for illumination control
9.5, external source selenium Na is added in culture medium2SeO3To final concentration 100mg/L, cultivate 5 days, obtain the spirulina rich in selenium, selenium has
Machine degree reaches 70.54%.
Aforesaid method, step 1) and 2) described in culture medium be ZarroukShi culture mediums (Tables 1 and 2).
The Zarrouk nutrient solution constituents of table 1
The Zarrouk trace element solution constituents of table 2
Aforesaid method, step 1) in inoculation spirulina after, the OD of algae solution560It is worth for 0.3.
In preceding method, with the phosphorus acid for adjusting pH value that mass concentration is 0.1mol/L, the spirulina is blunt top spirulina.
The present invention is excessive relative to spirulina to the nutrient of external source addition in algae solution, and main purpose is to improve battalion
The organic degree of foster element, its growth rate can decline, but not affect it to grow.
The present invention also provide by said method prepare rich in nutrient calcium, iron, zinc, selenium spirulina.
The present invention further provides application of the said method in regulation and control nutritious spirulina element accumulation.
The present invention is by the research environment factor (temperature, illumination, pH value) and spirulina absorption and accumulation calcium, iron, zinc and selenium unit
Element and its relation of growth, optimize the environmental condition of nutritious spirulina element absorption accumulation, and then it is certain to reach spirulina cells
On the premise of increment, Accumulation of nutrient element is set to reach maximum.I.e. by the regulation and control of environmental condition and outer liquid ion concentration
The dual track system of regulation and control, realizes the orientation accumulation of spirulina cells nutrient.This has important value in actual production.I.e. with
Using different geographical, the change of season environmental condition and photo-thermal combined breeding spirulina accumulate the orientation of realizing nutrient and are
The algae powder that Different Nutrient Elements content is high and its organic degree is high is produced in target, Instructing manufacture practice, makes Spirulina Products
Diversification, is not only greatly improved the economic benefit of enterprise, and overcomes the defect that current nutrient supplements, and promotes China's spiral
The development of algae industry and health industry.
The method that the present invention is provided is substantially increased the content of four kinds of nutrients of ferro-calcium-zinc-selenium in spirulina, and
Nutrient is combined with intracellular organic matter, reduce, to gastrointestinal irritation, improving absorption of human body utilization rate.Using this method
The content of the calcium iron zinc of the spirulina cells of culture has been respectively increased 97%, 175% and 87% than traditional cultural method；Calcium iron zinc
The organic degree of selenium is followed successively by：84.17%, 89.98%, 58.28% and 70.54%.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional meanses that are well known to those skilled in the art of technological means used, it is raw materials used to be commercial goods.
Embodiment 1
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 20 DEG C, illumination control is in 12klx, pH
External Calcium CaCO is added in control 8.5, in culture medium3To final concentration 0.6g/L, cultivate 3 days, obtain the spirulina rich in calcium
(calcium element content is 2.52mg/g in spirulina).The organic degree of calcium is 53.57%.Growth of spirulina platensis rate K=0.0465.
Embodiment 2
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 20 DEG C, illumination control is in 12klx, pH
External Calcium CaCO is added in control 8.5, in culture medium3To final concentration 0.6g/L, cultivate 5 days, obtain the spirulina rich in calcium
(calcium element content is 5.64mg/g in spirulina).The organic degree of calcium is 72.14%.Growth of spirulina platensis rate K=0.0448.
Embodiment 3
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 20 DEG C, illumination control is in 12klx, pH
External Calcium CaCO is added in control 8.5, in culture medium3To final concentration 0.6g/L, cultivate 7 days, obtain the spirulina rich in calcium
(calcium element content is 18.21mg/g in spirulina).The organic degree of calcium is 84.17%.Growth of spirulina platensis rate K=
0.0411。
Embodiment 4
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) in the culture spirulina of 3 days continue cultivate, temperature adjustment to 30 DEG C, illumination control in 4klx,
PH is controlled 10.5, and external source iron FeSO is added in culture medium4·7H2O to final concentration 40mg/L, cultivates 7 days, obtains and is rich in iron
Spirulina (in spirulina iron content be 1538.26ppm).The organic degree of iron is 84.95%.Growth of spirulina platensis rate
K=0.0332.
Embodiment 5
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 30 DEG C, illumination control is in 4klx, pH
External source iron FeSO is added in control 10.5, in culture medium4·7H2O to final concentration 40mg/L, cultivates 3 days, obtains rich in iron
Spirulina (iron content is 1634.34ppm in spirulina).The organic degree of iron is 89.98%.Growth of spirulina platensis rate K
=0.0411.
Embodiment 6
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 30 DEG C, illumination control is in 8klx, pH
External source zinc ZnSO is added in control 8.5, in culture medium4·7H2O to final concentration 40mg/L, cultivates 7 days, obtains the spiral shell rich in zinc
Rotation algae (Zn-ef ficiency content is 132.75ppm in spirulina).The organic degree of zinc is 5.34%.Growth of spirulina platensis rate K=
0.0376。
Embodiment 7
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 30 DEG C, illumination control is in 8klx, pH
External source zinc ZnSO is added in control 8.5, in culture medium4·7H2O to final concentration 40mg/L, cultivates 3 days, obtains the spiral shell rich in zinc
Rotation algae (Zn-ef ficiency content is 88.30ppm in spirulina).The organic degree of zinc is 58.28%.Growth of spirulina platensis rate K=
0.0429。
Embodiment 8
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5 so as to reach exponential phase.
2) by step 1) the middle culture spirulina of 3 days continuation culture, external source selenium Na is added in culture medium2SeO3It is dense to end
Degree 100mg/L, cultivates 7 days, obtains the spirulina rich in selenium (selenium element content is 350.27ppm in spirulina).Selenium it is organic
Change degree is 40.16%.Growth of spirulina platensis rate K=0.0246.
Embodiment 9
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5 so as to reach exponential phase.
2) by step 1) the middle culture spirulina of 3 days continuation culture, external source selenium Na is added in culture medium2SeO3It is dense to end
Degree 100mg/L, cultivates 3 days, obtains the spirulina rich in selenium (selenium element content is 399.70ppm in spirulina).Selenium it is organic
Change degree is 58.62%.Growth of spirulina platensis rate K=0.0277.
Embodiment 10
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5 so as to reach exponential phase.
2) by step 1) the middle culture spirulina of 3 days continuation culture, external source selenium Na is added in culture medium2SeO3It is dense to end
Degree 100mg/L, cultivates 5 days, obtains the spirulina rich in selenium (selenium element content is 1011.00ppm in spirulina).Selenium it is organic
Change degree is 70.54%.Growth of spirulina platensis rate K=0.0265.
Comparative example 1
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 30 DEG C,
Cultivate 3 days under the condition of culture of 4klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 30 DEG C, illumination control is in 4klx, pH
Control is cultivated 4 days 9.5, and the accumulation of calcium is 244.66ppm.Growth of spirulina platensis rate K=0.0852.
Comparative example 2
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 40 DEG C,
Cultivate 3 days under the condition of culture of 8klx, pH=8.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 40 DEG C, illumination control is in 8klx, pH
Control is cultivated 4 days 8.5, and the accumulation of iron is 192.64ppm.Growth of spirulina platensis rate K=0.0804.
Comparative example 3
1) spirulina is inoculated in ZShi culture mediums, after inoculation spirulina, the OD of algae solution560It is worth for 0.3.At 40 DEG C,
Cultivate 3 days under the condition of culture of 12klx, pH=9.5.
2) by step 1) the middle culture spirulina of 3 days continuation culture, temperature adjustment to 40 DEG C, illumination control is in 12klx, pH
Control is cultivated 4 days 9.5, and the accumulation of zinc is 30.06ppm.Growth of spirulina platensis rate K=0.0944.
From the above results, the present invention provide method can greatly improve several nutrients of spirulina content and
Organic degree, for reducing to GI irritation, improves absorption of human body utilization rate and provides effective means.
Although above with a general description of the specific embodiments the present invention is described in detail,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, without departing from theon the basis of the spirit of the present invention these modifications or improvements, belong to the scope of protection of present invention.
Claims (10)
1. it is a kind of regulation and control nutritious spirulina element accumulation method, it is characterised in that comprise the following steps：
1) spirulina is inoculated in culture medium, in temperature 20-40 DEG C, illumination 4-12klx, is trained under conditions of pH value 8.5-10.5
Support 3 days；
2) by step 1) in spirulina continue in temperature 15-35 DEG C, illumination 2-20klx is trained under conditions of pH value 8.5-11.0
Support, respectively by adding calcium, iron, zinc, selenium element to external source in culture medium, cultivate 3-7 days, obtain the spiral of high nutrition element
Algae.
2. method according to claim 1, it is characterised in that step 2) it is specific as follows：By step 1) in spirulina after
Continuous culture, adjusts temperature to 15-25 DEG C, and illumination control is controlled in 8.5-9.0 in 11-20klx, pH, in culture medium outside addition
Source CaCO3To final concentration 0.4-0.8g/L, incubation time is 3-7 days, obtains the spirulina rich in calcium.
3. method according to claim 1, it is characterised in that step 2) it is specific as follows：By step 1) in spirulina after
Continuous culture, temperature adjustment to 25-35 DEG C, illumination control is controlled in 10-11.0 in 2-6klx, pH, and in culture medium external source is added
FeSO4·7H2O to final concentration 30-60mg/L, cultivates 3-7 days, obtains the spirulina rich in iron.
4. method according to claim 1, it is characterised in that step 2) it is specific as follows：By step 1) in spirulina after
Continuous culture, temperature adjustment to 25-35 DEG C, illumination control is controlled in 8.5-9.5 in 6-11klx, pH, and in culture medium external source is added
ZnSO4·7H2O to final concentration 30-70mg/L, incubation time is 3-7 days, obtains the spirulina rich in zinc.
5. method according to claim 1, it is characterised in that step 2) it is specific as follows：By step 1) in spirulina after
Continuous culture, temperature adjustment to 28-35 DEG C, illumination control is controlled in 9.0-10.0 in more than 11klx, pH, is added in culture medium
External source selenium Na2SeO3To final concentration 80-120mg/L, incubation time is 3-7 days, obtains the spirulina rich in selenium.
6. method according to claim 1, it is characterised in that comprise the following steps：
1) spirulina is inoculated in culture medium, at 30 DEG C, 12klx is cultivated 3 days under conditions of pH value 9.5；
2) by step 1) in spirulina continue to cultivate, temperature adjustment to 20 DEG C, in 12klx, pH is controlled 8.5 for illumination control,
Add external source CaCO in culture medium3To final concentration 0.6g/L, cultivate 7 days, obtain the spirulina rich in calcium；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, illumination control in 4klx, pH controls 10.5, to
Add external source iron FeSO47H in culture medium2O to final concentration 40mg/L, cultivates 3 days, obtains the spirulina rich in iron；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, in 8klx, pH is controlled 8.5, Xiang Pei for illumination control
Add external source zinc ZnSO in foster base4·7H2O to final concentration 40mg/L, cultivates 3 days, obtains the spirulina rich in zinc；Or,
By step 1) in spirulina continue to cultivate, temperature adjustment to 30 DEG C, illumination control in 12klx, pH controls 9.5, to
Add external source selenium Na in culture medium2SeO3To final concentration 100mg/L, cultivate 5 days, obtain the spirulina rich in selenium.
7. the method according to any one of claim 1-6, it is characterised in that step 1) and 2) described in culture medium be
ZarroukShi culture mediums.
8. method according to claim 7, it is characterised in that step 1) in after inoculation spirulina, the OD of algae solution560It is worth and is
0.3。
9. the method according to any one of claim 1-6, it is characterised in that adjusted with the phosphoric acid that mass concentration is 0.1mol/L
Section pH value, the spirulina is blunt top spirulina.
10. according to any one of claim 1-9 methods described prepare rich in nutrient calcium, iron, zinc, selenium spirulina.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611267287.XA CN106635913B (en) | 2016-12-31 | 2016-12-31 | Method for regulating and controlling nutrient element accumulation of spirulina |
| CN201910261703.2A CN109825461A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina element zinc element accumulation |
| CN201910261367.1A CN110004103A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina elemental iron element accumulation |
| CN201910261345.5A CN109837234A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina element calcium element accumulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611267287.XA CN106635913B (en) | 2016-12-31 | 2016-12-31 | Method for regulating and controlling nutrient element accumulation of spirulina |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910261367.1A Division CN110004103A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina elemental iron element accumulation |
| CN201910261703.2A Division CN109825461A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina element zinc element accumulation |
| CN201910261345.5A Division CN109837234A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina element calcium element accumulation |
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| Publication Number | Publication Date |
|---|---|
| CN106635913A true CN106635913A (en) | 2017-05-10 |
| CN106635913B CN106635913B (en) | 2020-06-09 |
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| CN201611267287.XA Active CN106635913B (en) | 2016-12-31 | 2016-12-31 | Method for regulating and controlling nutrient element accumulation of spirulina |
| CN201910261367.1A Pending CN110004103A (en) | 2016-12-31 | 2016-12-31 | A method of regulation nutritious spirulina elemental iron element accumulation |
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| CN108159086A (en) * | 2018-01-22 | 2018-06-15 | 暨南大学 | A kind of spirulina bioconversion natural nano selenium and preparation method and application |
| CN108587984A (en) * | 2018-06-08 | 2018-09-28 | 武汉藻优生物科技有限公司 | A kind of selenium enriched Spirulina culture, its cultural method and application |
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| CN102876579A (en) * | 2012-08-03 | 2013-01-16 | 安徽大学 | Method for culturing and detecting high selenium-enriched spirulina platensis |
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| CN1063786C (en) * | 1995-09-05 | 2001-03-28 | 中国科学院武汉植物研究所 | Manufacturing method of iron-rich pure spirulina |
| CN102517244B (en) * | 2011-12-31 | 2014-02-19 | 北京林业大学 | Method for improving metallothionein content of spirulina |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108159086A (en) * | 2018-01-22 | 2018-06-15 | 暨南大学 | A kind of spirulina bioconversion natural nano selenium and preparation method and application |
| CN108159086B (en) * | 2018-01-22 | 2020-02-07 | 暨南大学 | Spirulina biotransformation natural nano-selenium and preparation method and application thereof |
| CN108587984A (en) * | 2018-06-08 | 2018-09-28 | 武汉藻优生物科技有限公司 | A kind of selenium enriched Spirulina culture, its cultural method and application |
| CN108587984B (en) * | 2018-06-08 | 2022-03-15 | 武汉藻优生物科技有限公司 | Selenium-rich spirulina culture, and culture method and application thereof |
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| CN106635913B (en) | 2020-06-09 |
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