CN105316237B - A kind of chlorella efficiently cultivates the composition of culture solution - Google Patents
A kind of chlorella efficiently cultivates the composition of culture solution Download PDFInfo
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- CN105316237B CN105316237B CN201510848969.9A CN201510848969A CN105316237B CN 105316237 B CN105316237 B CN 105316237B CN 201510848969 A CN201510848969 A CN 201510848969A CN 105316237 B CN105316237 B CN 105316237B
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Abstract
The present invention provides a kind of chlorella cultivation culture solution composition, it is characterized by comprising, based on weight percent: niacin 0.01~0.02%, urea 70~75%, sodium nitrate 3.5~4.5%, anhydrous calcium chloride 3.5~4.5%, epsom salt 9~12%, dipotassium hydrogen phosphate 4~5%, ferric citrate 0.8~0.9%, citric acid 0.8~0.9%, natrium carbonicum calcinatum 2~3%, b diammonium disodium edta 0.1~0.2%, boric acid 0.3~0.4%, tetrahydrate manganese chloride 0.2~0.3%, white vitriol 0.02~0.04%, Sodium Molybdate Dihydrate 0.04~0.06%, cupric sulfate pentahydrate 0.01~0.02%, cobalt nitrate hexahydrate 0.005~0.007%.
Description
Technical field
The present invention relates to a kind of chlorellas to cultivate culture solution composition, and preparation method thereof.
Background technique
Chlorella has about 10 kinds of chlorellas in the market current, is all rich in protein, unsaturated fatty acid, class
There is a variety of important life active compounds such as carrotene, vitamin and the chlorella factor high nutritive value and raising to exempt from
The function of epidemic disease power has been cited as the healthy food of the 21st century mankind.With the increasingly raising of people's living standard, to bead
The demand of algae can be increasing.
It is generally acknowledged that Chlorella, in rudimentary plant, the main inorganic carbon that absorbs passes through photosynthesis synthesis of organic substance.But it adopts
With illumination autotrophy cultural method, after frustule concentration increases to certain amount, necessarily stops light into people's culture, make to train
The frustule concentration raised is very low.
Since nineteen seventies, foreign scholar's discovery, some chlorellas can be grown under non-illuminated conditions
Organic matter, is not only greatly improved the speed of growth of chlorella, but also obtains very big algae biomass.
Summary of the invention
It is an object of the invention to filter out can heterotrophic growth chlorella vulgaris, and determine obtain superelevation cell concentration bead
The optimization culture control condition of algae realizes that superelevation cell is dense to solve the problems, such as that chlorella is difficult to turn out superelevation cell concentration
Spend the industrialization production of chlorella culture.
An object of the present invention provides a kind of chlorella cultivation culture solution composition, it is characterised in thatIncluding being based on Weight percent meter:
Niacin 0.01~0.02%, urea 70~75%, sodium nitrate 3.5~4.5%, anhydrous calcium chloride 3.5~
4.5%, epsom salt 9~12%, dipotassium hydrogen phosphate 4~5%, ferric citrate 0.8~0.9%, citric acid 0.8~
0.9%, natrium carbonicum calcinatum 2~3%, b diammonium disodium edta 0.1~0.2%, boric acid 0.3~0.4%, tetrahydrate manganese chloride
0.2~0.3%, white vitriol 0.02~0.04%, Sodium Molybdate Dihydrate 0.04~0.06%, cupric sulfate pentahydrate 0.01~
0.02%, cobalt nitrate hexahydrate 0.005~0.007%.
As one of preferred embodiment, the present invention provides a kind of chlorellas to cultivate culture solution composition, packet
Include niacin 1g, urea 5290g, sodium nitrate 300g, anhydrous calcium chloride 295g, epsom salt 750g, dipotassium hydrogen phosphate 360g,
Ferric citrate 60g, citric acid 60g, natrium carbonicum calcinatum 200g, b diammonium disodium edta 10g, boric acid 28.6g, four water chlorinations
Manganese 18.1g, white vitriol 2.22g, Sodium Molybdate Dihydrate 3.9g, cupric sulfate pentahydrate 0.79g, cobalt nitrate hexahydrate 0.49g.
An object of the present invention provides a kind of method for preparing chlorella cultivation culture solution composition, including following
Step:
(20~25 DEG C) at normal temperature take niacin 1g, urea 5290g, sodium nitrate 300g, anhydrous calcium chloride 295g, seven
Water magnesium sulfate 750g, dipotassium hydrogen phosphate 360g, ferric citrate 60g, citric acid 60g, natrium carbonicum calcinatum 200g, second diammonium tetrem
Acid disodium 10g, boric acid 26.6g, tetrahydrate manganese chloride 18.1g, white vitriol 2.22g, Sodium Molybdate Dihydrate 3.9g, cupric sulfate pentahydrate
0.79g, cobalt nitrate hexahydrate 0.49g, pour into the container for filling 10L water, stir evenly, and become signified chlorella of the invention
Cultivate the composition of culture solution.
Beneficial effect
1, the present invention increases b diammonium disodium edta in conventional formulation, which, which is that one kind is good in chemistry, matches
Mixture, there are six coordination atom, the complexs of formation to be called chelating object for it.Using heavy metal detoxification can be made in chlorella cultivation
Medicine, complexing agent, antioxidant synergist, stabilizer and softening agent etc..
2, while sodium nitrate is increased, sodium nitrate is in chlorella cultivates using the quick-acting fertilizer that can be used as acidity.
3, this formula also add niacin.Niacin is broad spectrum type plant growth regulator, is called NAA.It can promote small
The growth of ball algae, induction, stimulation and quickening chlorella cells division.
4, the present invention has the advantages that using heterotrophism batch or fermented and cultured screening chlorella-USTB01, Ke Yi
The frustule dry weight concentrations turned out are made within 120 hours to reach 20-50g/L, entire incubation is completely in artificial controllable temperature
Degree and aseptic condition under carry out, solve it is low using pond culture chlorella cells concentration, be easy to be bacterial contamination and by weather
Variation influences big disadvantage.By the chlorella cells turned out after harvest, drying and tabletting, human health food can be used as
Product or pharmaceuticals are applied.
Specific embodiment
The following examples are further illustrations of the invention, but never it limits the scope of the present invention.Referring to
Embodiment is further elaborated on the present invention, it should be appreciated to those skilled in the art that the present invention is not limited to these implementations
Example and the preparation method used.Moreover, those skilled in the art's description according to the present invention can be equal to the present invention
Replacement, combination, improvement or modification, but these are intended to be included in the scope of the present invention.
Embodiment 1
(20~25 DEG C) at normal temperature take niacin 1g, urea 5290g, sodium nitrate 300g, anhydrous calcium chloride 295g, seven
Water magnesium sulfate 750g, dipotassium hydrogen phosphate 360g, ferric citrate 60g, citric acid 60g, natrium carbonicum calcinatum 200g, second diammonium tetrem
Acid disodium 10g, boric acid 26.6g, tetrahydrate manganese chloride 18.1g, white vitriol 2.22g, Sodium Molybdate Dihydrate 3.9g, cupric sulfate pentahydrate
0.79g, cobalt nitrate hexahydrate 0.49g.It pours into the container for filling 10L water, stirs evenly, then taking 1 liter of concentration is 20-50g/L
Chlorella vulgaris into the above water container, be placed on it is outdoor, then, ventilation 10 days after, detected the bead in the above water container
Concentration of algae reaches 50-70g/L.
Reference examples 1
According to patent document200510086288XPreparation.
Glucose 40g, urea 0.2g, K2HPO4 0.04g、MgSO4.7H2O 0.075g、CaCl2.7H2O 0.036g、
Na2CO30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution A5 1ml, water 1000ml, H3BO4
2.86g、MnCl2.4H2O 1.81g、ZnSO4 0.222g、Na2MoO4 0.39g、CuSO4.5H2O 0.079g、Co(NO3)2.6H2O 49.4g。
Present invention uses b diammonium disodium edta, which is a kind of good compounding agent in chemistry, there are six it
Coordination atom, the complex of formation are called chelating object.Using heavy metal detoxification medicine, complexing agent, anti-can be made in chlorella cultivation
Oxygen synergist, stabilizer and softening agent etc..And reference examples useless
Present invention uses sodium nitrate, the raw material is in chlorella cultivates using the quick-acting fertilizer that can be used as acidity.
Present invention uses-niacin, which is broad spectrum type plant growth regulator, is called-NAA.It can promote bead
The growth of algae, induction, stimulation and accelerates chlorella cells division, to reach high-yield and high-efficiency, be the maximum bright spot of the present invention and
Reference examples are useless.
The culture solution of bead algae culturing liquid of the invention and reference examples (200510086288X), should be in the same way
When cultivating chlorella, through detecting, it can reach using the bead concentration of algae in the water container of bead algae culturing liquid culture of the invention50~70G/L, and applying the bead concentration of algae in the water container of the culture solution culture of reference examples (200510086288X) is only 20~30g/L.As it can be seen thatEfficiency of the invention is apparently higher than reference examples。
Claims (1)
1. a kind of chlorella cultivates culture solution composition, which is characterized in that be prepared using following methods:
20-25 DEG C at a temperature of, take niacin 1g, urea 5290g, sodium nitrate 300g, anhydrous calcium chloride 295g, seven water sulfuric acid
Magnesium 750g, dipotassium hydrogen phosphate 360g, ferric citrate 60g, citric acid 60g, natrium carbonicum calcinatum 200g, b diammonium disodium edta
10g, boric acid 26.6g, tetrahydrate manganese chloride 18.1g, white vitriol 2.22g, Sodium Molybdate Dihydrate 3.9g, cupric sulfate pentahydrate 0.79g,
Cobalt nitrate hexahydrate 0.49g is poured into the container for filling 10L water, is stirred evenly,.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107384832B (en) * | 2017-08-28 | 2020-06-09 | 中国科学院水生生物研究所 | Method for culturing nitrogen-fixing blue algae in large quantity at low cost |
| CN107667934B (en) * | 2017-08-31 | 2018-08-31 | 海南良之缘生物科技有限公司 | The cultural method of South America prawn |
| CN110835280B (en) * | 2019-11-20 | 2022-06-10 | 中国科学院南京土壤研究所 | Method for fertilizer conservation and yield increase of rice field and interception of nitrogen and phosphorus loss and biological membrane culture medium composition |
| CN115026128B (en) * | 2022-06-17 | 2023-08-18 | 中国科学院南京土壤研究所 | Rice field heavy metal resistance control and digestion method based on periphyton |
Citations (3)
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|---|---|---|---|---|
| CN1766082A (en) * | 2005-08-25 | 2006-05-03 | 北京科技大学 | Culturing method for heterotrophic chlorella growth without irradiation |
| CN103045709A (en) * | 2011-10-14 | 2013-04-17 | 中国科学院烟台海岸带研究所 | Method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions |
| CN105002093A (en) * | 2015-02-02 | 2015-10-28 | 王兆伟 | Chlorella pacifica medium |
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- 2015-11-20 CN CN201510848969.9A patent/CN105316237B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766082A (en) * | 2005-08-25 | 2006-05-03 | 北京科技大学 | Culturing method for heterotrophic chlorella growth without irradiation |
| CN103045709A (en) * | 2011-10-14 | 2013-04-17 | 中国科学院烟台海岸带研究所 | Method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions |
| CN105002093A (en) * | 2015-02-02 | 2015-10-28 | 王兆伟 | Chlorella pacifica medium |
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| EDTA二钠对培养单胞藻的影响;姚久祥等;《广西水产科技》;20071231(第1期);全文 |
| NAA对小球藻生长及叶绿素和蛋白质含量的影响;吕富等;《盐城工学院学报（自然科学版）》;20060630;第19卷(第2期);摘要 |
| 硝酸钠和氯化铵对小球藻生长的影响;吴旭等;《河北渔业》;20081231(第11期);全文 |
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