CN104957066B - A kind of one three using Pteria penguin three type pearls of cultivation uses method - Google Patents
A kind of one three using Pteria penguin three type pearls of cultivation uses method Download PDFInfo
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- CN104957066B CN104957066B CN201510399548.2A CN201510399548A CN104957066B CN 104957066 B CN104957066 B CN 104957066B CN 201510399548 A CN201510399548 A CN 201510399548A CN 104957066 B CN104957066 B CN 104957066B
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- pearl
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- pteria penguin
- penguin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of one three for cultivating three type pearls using Pteria penguin to use method.First, pearl core and the free pearl of mantle piece culture are implanted into the left bag core position of pearly shellfish；It plunges into the commercial sea after cultivation a period of time, is detected whether to retain pearl core with X ray machines, the Pteria penguin to retaining pearl core is implanted into mantle piece suspension culture grain of rice pearl in right bag core position by the method for injection；Meanwhile moulding mould core culture blister pearl is implanted under its outer embrane；It grows cultured pearls using a period of time cultivation, you can dissociate pearl, grain of rice pearl and blister pearl are harvested in a pearly shellfish.Present invention employs Pteria penguin one three to use technology, considerably improves its yield of growing cultured pearls, improves the economic benefit of pearl culture, therefore has good application prospect.
Description
Technical field
The present invention relates to pearl cultivating technical field more particularly to it is a kind of utilize Pteria penguin cultivate three type pearls one
Body three uses method.
Background technology
Currently, the cultivation of Pteria penguin is grown cultured pearls in production, typically single mould core of being planted under outer embrane is cultivated respectively
The blister pearl of kind shape, economic benefit are relatively low.In addition, circular dissociates, there is also pearly shellfishes to spit core rate for the culture technique of pearl
The problems such as height, the death rate are high, bead yield rate is low seriously restricts the development of Pteria penguin industrialization cultivation.Pteria penguin has
There is the features such as speed of growth is fast, survival rate is high, secretion nacre speed is fast, thus is also relatively suitble to culture grain of rice pearl.
Xie Shaohe etc. did research for the dual-purpose feasibility of the one of Pteria penguin.First pass through slotting core operation implantation pearl core
And mantle piece, the pearl that dissociates is cultivated, after 6 months, by attaching the attached shell pearl of shell mold core culture.After continuing cultivation 1 year, pearl is taken.Hand
Art shellfish blister pearl bead yield rate is 69%, but free pearl culture core-remaining rate is only 20%.Thus, they think Pteria penguin not
It is adapted to cultivate free pearl.[Xie Shaohe, Deng Chenmao, beam flying dragon wait the integrally dual-purpose engineering test of growing cultured pearls of Pteria penguins
Study [J] Guangdong Ocean University journal, 2012,32 (6)：33—38]
It is existing to plant core in internal sac and cultivate the operation method of pearl and immature of dissociating, geneva is substantially indiscriminately imitated completely
The plant kernel method of nacre, but since Pteria penguin enteron aisle is bent, byssus is luxuriant, and core position is smaller.It is implanted into aforementioned manners
Pearl core is sent to core position by internal organ byssus upper side, due to big by byssus barrier operating difficulty, in addition, due to penguin pearl
Shellfish paw withdrawal muscular strength amount is larger, and when contraction, easily pearl core was discharged, and the core-remaining rate that causes to perform the operation is low.So Pteria penguin internal sac is planted
Core grow cultured pearls at home, outer do not form large-scale production.Existing mould core of being planted under outer embrane cultivates blister pearl, mainly trains
Semi-circular blister pearl is educated, comparative maturity, bead yield rate and commodity pearl rate are all guaranteed for operating method.
Currently, not yet with the report of Pteria penguin culture grain of rice pearl.Mantle piece is in implantation pearly shellfish internal sac
Afterwards, no nucleus pearl can be formed, but not of uniform size without nucleus pearl, shape is mostly flat long and narrow, this just loses the value in market.
The preparation that this key to the issue is mantle piece is solved, how to ensure its size and shape and consistency.
Invention content
In view of the above-mentioned problems, the present invention provides a kind of three use sides of one cultivating three type pearls using Pteria penguin
Method.
The present invention is technical solution used by solving its technical problem：
A kind of method of one three for cultivating three type pearls using Pteria penguin, it is characterised in that include the following steps：
Step 1 chooses the good Pteria penguin of upgrowth situation as pearly shellfish and piece shell, wherein the pearly shellfish
The processing of bolt mouth need to be done；
Step 2, the integral sleeve film epithelial tissue of piece shell described in obtaining step one, removes the skirt of the outer embrane
Purified treatment is carried out after band part, and remainder is then cut into the consistent mantle piece D1 of specification, is used in combination at merbromin liquid medicine
Reason；
Step 3 obtains the Pteria penguin pearl core of certain diameter specification；
Small pieces D1 and pearl core are sequentially inserted into pearly shellfish core position by step 4, will by the way of first sending small pieces to send pearl core again
The mantle piece D1 and pearl core are inserted into the left bag core position of pearly shellfish, and the mantle piece D1 and pearl core are adjacent to, with training
Educate the free pearl of circular；
Step 5 waits for recuperating certain time after the completion of aforesaid operations, then plunge into the commercial sea cultivation certain time；
Core situation is stayed in step 6, detection, and the Pteria penguin with pearl core is then by spare；
Step 7, the integral sleeve film epithelial tissue of piece shell described in obtaining step one, removes the skirt of the outer embrane
Purified treatment is carried out after band part, and remainder is then cut into the consistent mantle piece D2 of specification, is used in combination at merbromin liquid medicine
Reason, is then mixed in balanced salt solution, it is spare that cell suspension is made；
Step 8, by cell suspension injection Pteria penguin right bag core position, to cultivate grain of rice pearl；
Step 9 is implanted into moulding mould core, for cultivating attached shell moulding pearl under the outer embrane of the pearly shellfish；
Postoperative Pteria penguin is placed back in Cultivated water cultivate and be grown cultured pearls, cultivates certain time by step 10
Afterwards, the free pearl of circular, grain of rice pearl and attached shell moulding pearl can be harvested from Pteria penguin.
As further improvement of these options, in step 1, the bolt mouth processing further comprises waiting for penguin pearl
After shellfish opens shell, opening is clogged with wooden wedge.
Further, the consistent mantle piece D1 of the specification is the rectangular mantle pieces of 2mm.
Further, in step 6, core situation is stayed using the detection of X- ray machines.
Further, the consistent mantle piece D2 of the specification is the rectangular mantle pieces of 1mm.
Further, in step 9, it is implanted into moulding mould core under the outer embrane of the pearly shellfish and further comprises with operation work
Its edge outer embrane is sent the patch shell membrane edge at core suitably to be raised with shell by tool, with dry cloth or paper by the shell under outer embrane
Inside is dried, and in the one side coated with adhesive of moulding mould core, is sent into shell with tweezers, is adjacent to the shell position after drying.
Further, it in step 5, waits for recuperating 20 days after the completion of aforesaid operations, cultivation 6 months of then plunging into the commercial sea.
Further, the Pteria penguin of shell a height of 10cm is chosen as pearly shellfish, and the penguin pearl of a height of 6~7cm of shell
Shellfish is as piece shell.
Further, it in step 10, after the completion of surgical procedure, recuperates 20 days, fills cage, continue cultivation of plunging into the commercial sea, after 1 year, you can
Take pearl.
The beneficial effects of the invention are as follows：The present invention is used as a kind of one three for being cultivated three type pearls using Pteria penguin
Method, because of the free pearl of the left pocket planting core culture of Pteria penguin, right pocket planting enters mantle piece culture grain of rice pearl, in this way, keeping penguin precious
The left bag of pearl shellfish has played effect simultaneously with right bag, in addition Pteria penguin is suitble to cultivate attached shell elephant shape pearl, therefore, fully again
The function of growing cultured pearls of having played Pteria penguin increases the economic benefit of cultivation Pteria penguin.The culture of grain of rice pearl is using note
The mode for penetrating mantle piece suspension while improving efficiency, reduces the injury to pearly shellfish, improves survival rate；It is free
Pearl culture makees piece shell using smaller Pteria penguin, since its outer embrane is thin, is easy cutting, while because of small pieces made of it
It is more flat to be easy closely to fit to pearl core surface, avoid the occurrence of tail pearl；Geneva pearl can also be used in free pearl and the culture of grain of rice pearl
The pearl of female Bei Zuowei piece shells, output is white, and colour consistency is good, therefore has good application prospect.
Specific implementation mode
Below by way of specific embodiment, the invention will be further described, but protection scope of the present invention is not limited to this：
A kind of method of one three for being cultivated three type pearls using Pteria penguin, is included the following steps：Step 1 is chosen
The good Pteria penguin of upgrowth situation is as pearly shellfish and piece shell, wherein the pearly shellfish need to do the processing of bolt mouth；Step
Two, the integral sleeve film epithelial tissue of piece shell described in obtaining step one carries out after removing the opotism part of the outer embrane
Then remainder is cut into the consistent mantle piece D1 of specification by purified treatment, merbromin liquid medicine is used in combination to handle；Step 3 obtains
Take the Pteria penguin pearl core of certain diameter specification；Small pieces D1 and pearl core are sequentially inserted into pearly shellfish core position, using elder generation by step 4
The left bag core position for sending small pieces to send the mode of pearl core that the mantle piece D1 and pearl core are inserted into pearly shellfish again, and make the cell
Small pieces D1 and pearl core are adjacent to, to cultivate the free pearl of circular；Step 5 waits for recuperating certain time after the completion of aforesaid operations, so
After plunge into the commercial sea cultivation certain time；Core situation is stayed in step 6, detection, and the Pteria penguin with pearl core is then by spare；Step 7,
The integral sleeve film epithelial tissue of piece shell described in obtaining step one, is purified after removing the opotism part of the outer embrane
Processing, then cuts into the consistent mantle piece D2 of specification by remainder, is used in combination merbromin liquid medicine to handle, is then mixed in balance
In salting liquid, it is spare that cell suspension is made；Step 8, by cell suspension injection Pteria penguin right bag core position, to cultivate
Grain of rice pearl；Step 9 is implanted into moulding mould core, for cultivating attached shell moulding pearl under the outer embrane of the pearly shellfish；Step
Ten, postoperative Pteria penguin is placed back in Cultivated water cultivate and is grown cultured pearls, cultivation after a certain period of time, can be from penguin treasure
Pearl shellfish harvests the free pearl of circular, grain of rice pearl and attached shell moulding pearl.
Specially：
It selects the healthy Pteria penguin of a height of 10cm of shell or so to make pearly shellfish, selects the health of a height of 6~7cm of shell or so
Pteria penguin is as piece shell.
Bolt mouth will be carried out after the completion of pearly shellfish cleaning, is realized using following processing：By the intensive proper alignment of pearly shellfish in side
It shapes in material frame, twisting part prevents it from being open under, and temporarily supports 1 to 2 hours, then, takes out wherein several shellfishes, makes it
Remaining shellfish has out the space of shell, is inserted into wooden wedge after it opens shell naturally.Specifically, use specification for 60cm × 30cm ×
The plastics box of 20cm, can once handle 50 or so pearly shellfishes, if there is the untight place of arrangement, can use wooden wedge etc.
Filling.
Using first sending small pieces, then send the mode of pearl core, be inserted into Pteria penguin left bag core position, use specification for 1.8 pearl
Core（0.4-0.5cm）.It is as follows：Opener opens operated shellfish, and byssus is cut with scissors（Sight and notch are not influenced）.
Byssal foramen and downward slight amount are caught on pulling needle, the fold of black and white handing-over is crosscutting above the base portion of foot, knife edge length and pearl
Nuclear diameter is roughly the same, and has certain radian.Then, with knife to surface channel is opened along epidermis.With sending the piece needle will be small
Piece is sent to the bottom of the channel right over notch, pearl core is equally then sent to the bottom of the channel, is slightly exerted oneself along the right side of paw withdrawal flesh
It is close to small pieces.（Left bag）
It is the surgical procedure of free pearl culture above, after being fully completed, and recuperates 20 days, dress cage is plunged into the commercial sea cultivation.It is marine
After cultivation 6 months, the Pteria penguin grown cultured pearls before is pulled out, stays core situation using the detection of X- ray machines, the penguin with pearl core
Pearl shell, then carry out grain of rice pearl and the culture operation of attached shell pearl.
The outer embrane epithelial tissue of piece shell is taken out with conventional method, removes opotism part, after purified treatment, is cut into 2mm
The small pieces of × 2mm are used in combination merbromin liquid medicine to handle.The outer embrane epithelial tissue of piece shell is taken out with conventional method, removes opotism portion
Point, after purified treatment, 1mm or less small pieces are cut into, after being handled with merbromin liquid medicine, are all mixed in balanced salt solution, are made thin
Born of the same parents' small pieces suspension is spare, and die density is maintained at 5 to 8 every milliliter in suspension.
It is placed on operation bracket after the pearly shellfish of core will be stayed to fasten mouth, 3ml or so mantle piece suspension, syringe needle is drawn with syringe
Right bag position is reached by notch, is slowly all injected into core position.
Meanwhile the patch shell membrane edge at core is sent suitably to be raised with shell on its edge outer embrane with operation tool；With dry
Cloth or paper will be dried inside the shell under outer embrane, and in the one side coated with adhesive that moulding mould core is smooth, shell is sent into tweezers
It is interior, it is adjacent to the shell position after drying.
After the completion of grain of rice pearl and attached shell pearl culture surgical procedure, recuperates 20 days, fill cage, continue cultivation of plunging into the commercial sea.After 1 year,
Pearl is taken, in this way, a pearly shellfish can harvest the free pearl of attached shell pearl, circular and grain of rice pearl simultaneously.
It should be noted that free pearl and the culture of grain of rice pearl also can be used pteria martensii as piece shell.Operation side
Method is identical as above description.
The preferable implementation of the present invention is illustrated above, certainly, the present invention can also use and above-mentioned implementation
The different form of mode and similar field, these do not constitute any restrictions to present embodiment, are familiar with this field
Technical staff under the premise of without prejudice to spirit of that invention made by equivalent transformation or corresponding modification, should all belong to this hair
In bright protection domain.
Claims (7)
1. a kind of method of one three for cultivating three type pearls using Pteria penguin, it is characterised in that include the following steps：
Step 1 chooses the good Pteria penguin of upgrowth situation as pearly shellfish and piece shell, wherein the pearly shellfish need to be done
The processing of bolt mouth；
Step 2, the integral sleeve film epithelial tissue of piece shell described in obtaining step one, removes the opotism portion of the outer embrane
Purified treatment is carried out after point, remainder is then cut into the consistent mantle piece D1 of specification, merbromin liquid medicine is used in combination to handle；
Step 3 obtains the Pteria penguin pearl core of certain diameter specification；
Small pieces D1 and pearl core are sequentially inserted into pearly shellfish core position by step 4, will be described by the way of first sending small pieces to send pearl core again
Mantle piece D1 and pearl core are inserted into the left bag core position of pearly shellfish, and the mantle piece D1 and pearl core are adjacent to, to cultivate just
The free pearl of circle, wherein the consistent mantle piece D1 of the specification is the rectangular mantle pieces of 2mm；
Step 5 waits for recuperating certain time after the completion of aforesaid operations, then plunge into the commercial sea cultivation certain time；
Core situation is stayed in step 6, detection, and the Pteria penguin with pearl core is then by spare；
Step 7, the integral sleeve film epithelial tissue of piece shell described in obtaining step one, removes the opotism portion of the outer embrane
Purified treatment is carried out after point, remainder is then cut into the consistent mantle piece D2 of specification, is used in combination merbromin liquid medicine to handle, so
After be mixed in balanced salt solution, be made that cell suspension is spare, wherein the consistent mantle piece D2 of the specification is 1mm rectangular thin
Born of the same parents' small pieces；
Step 8, by cell suspension injection Pteria penguin right bag core position, to cultivate grain of rice pearl；
Step 9 is implanted into moulding mould core, for cultivating attached shell moulding pearl under the outer embrane of the pearly shellfish；
Postoperative Pteria penguin is placed back in Cultivated water cultivate and be grown cultured pearls by step 10, and cultivation after a certain period of time, can
From the free pearl of Pteria penguin harvest circular, grain of rice pearl and attached shell moulding pearl.
2. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：In step 1, the bolt mouth processing further comprises after Pteria penguin opens shell, and opening is clogged with wooden wedge.
3. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：In step 6, core situation is stayed using the detection of X- ray machines.
4. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：In step 9, under the outer embrane of the pearly shellfish being implanted into moulding mould core further comprises its edge with operation tool
Outer embrane send the patch shell membrane edge at core suitably to be raised with shell, will be dried inside the shell under outer embrane with dry cloth or paper,
In the one side coated with adhesive of moulding mould core, it is sent into shell with tweezers, is adjacent to the shell position after drying.
5. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：In step 5, wait for recuperating 20 days after the completion of aforesaid operations, cultivation 6 months of then plunging into the commercial sea.
6. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：The Pteria penguin of a height of 10cm of shell is chosen as pearly shellfish, and the Pteria penguin of a height of 6~7cm of shell is as small pieces
Shellfish.
7. a kind of method of one three for cultivating three type pearls using Pteria penguin according to claim 1, feature
It is：In step 10, after the completion of surgical procedure, recuperates 20 days, fill cage, continue cultivation of plunging into the commercial sea, after 1 year, you can take pearl.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111771784A (en) * | 2020-08-07 | 2020-10-16 | 广东荣辉珍珠养殖有限公司 | Method for producing blister pearl and regenerative cell bead by using pinctada martensii golden-lip pearl culturing shell |
| CN112673999B (en) * | 2020-12-31 | 2022-02-11 | 广东尊鼎珍珠有限公司 | Method for cultivating conjoined calabash pearls by using one-shell-one-nuclear-position |
| CN113207768B (en) * | 2021-06-07 | 2022-11-04 | 北部湾大学 | Novel method for cultivating free pearls of pteria penguin |
| CN113693000A (en) * | 2021-09-23 | 2021-11-26 | 广东海洋大学 | Production method of steel gray pearls |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101331859B (en) * | 2008-08-06 | 2012-03-28 | 谢绍河 | Integration double-purpose efficient bead breeding method of penguin pearl shell and nucleus sending device thereof |
| CN101455187A (en) * | 2009-01-08 | 2009-06-17 | 梁盛 | Sea-water culturing method of pteria penguin bronze-coloured free pearls |
| CN101491228B (en) * | 2009-03-02 | 2012-07-04 | 广西中医学院 | Sea no-nucleus pearl incubation method |
| CN102217563B (en) * | 2011-04-11 | 2012-10-03 | 海南京润珍珠生物技术股份有限公司 | Nucleus inserting method for nuclear-free pearl grain breeding |
| CN103340168B (en) * | 2013-07-03 | 2014-09-03 | 浙江海洋学院 | Special fresh water pearl cultivating method based on small seawater shellfish cell pieces |
| CN103314912B (en) * | 2013-07-03 | 2014-11-26 | 浙江海洋学院 | Method of producing pearls on the basis of pearl shell mantel cell culture |
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