CN104304104A - Method for preparing monomer oyster fries by utilizing vertical growth characteristics of oysters - Google Patents

Method for preparing monomer oyster fries by utilizing vertical growth characteristics of oysters Download PDF

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Publication number
CN104304104A
CN104304104A CN201410481832.XA CN201410481832A CN104304104A CN 104304104 A CN104304104 A CN 104304104A CN 201410481832 A CN201410481832 A CN 201410481832A CN 104304104 A CN104304104 A CN 104304104A
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oyster
seed
seedling
shell
juvenile mollusk
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CN104304104B (en
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张跃环
喻子牛
苏家齐
张扬
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South China Sea Institute of Oceanology of CAS
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South China Sea Institute of Oceanology of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)

Abstract

The invention discloses a method for preparing monomer oyster fries by utilizing the vertical growth characteristics of oysters. The method utilizes the vertical growth characteristics of oyster spats under high density (2-3 pieces/ml) and combines the individual natural falling-off phenomenon of the spats of 20-30 mm; a lot of healthy and complete mid-size monomer oyster fries can be obtained through high-density seedling collection, spat cultivation, spat off-base and other technical means. The monomer oyster fries obtained by the method are different from monomer oyster fries with flat right shells which are obtained by a corrugated plate, a plastic plate and the like and grow in a creeping way, have the original shell forms of the oysters and have more attractive appearance and higher economic values. The monomer oyster fries obtained by the method can be directly cultivated by the utilization of a silk screen mesh cage with a mesh of above 8 mm, an intermediate cultivation process in which a mesh bag with a mesh of less than 8 mm is utilized is avoided, and the cultivation links of the monomer oyster are simplified. The monomer oyster fries have the advantages of being high in growth speed, survival rate, yield and the like.

Description

A kind of method utilizing oyster vertical growth characteristic to prepare cultchless oyster seed
Technical field:
The invention belongs to technical field of shellfish culture in Marine agriculture, be specifically related to a kind of method utilizing oyster vertical growth characteristic to prepare cultchless oyster seed.
Background technology:
Oyster belongs to Mollusca, Bivalvia, pearl shell order, Ostreidae, is global distribution monoid; Oyster meat delicious, be rich in multiple essential amino acid, a kind of important living marine resources, one of most important mariculture grass economic category in the world especially, its cultivation gross yield and yield per unit area rank first (Guo etc., 2006) at all shellfish culture kind metas.China is oyster culture big country, and 2009-2012 annual production all 350-380 about ten thousand tons, accounts for 35% of cultivated shellfish output, 25% of mariculture gross yield, more than 70% (China Fisheries yearbook, 2013) of world wide production.
To cultivate kind the same with other, in the breeding process of oyster, also often occurs the bad phenomenon such as disease-resistant resistance is poor, individuality miniaturization, specific yield reduction.To this, except breeding environment, aquaculture model is most important, China's oyster culture can be divided into assembling type cultivation and monomer cultivate two kinds of patterns.Wherein, assembling type cultivates, and normal multiple oyster anchors at together, and due to the restriction of growing space, hull shape irregular, have impact on attractive in appearance widely; Also cause the competition of oyster on food, affect its growth rate.The restriction of cultchless oyster due to its free property not by growing space, thus hull shape rule is attractive in appearance, and size is even, be easy to put in a suitable place to breed and gather in the crops, and economic worth is higher, extensively adopts abroad.
Cultchless oyster seed source usually depends on chemicals process and obtains, and this method directly forms monomer after can making larval metamorphosis.But; the cultchless oyster juvenile mollusk that this method obtains will be cultivated from 0.35-0.40mm, there is not enough (Zhang Zhi bosom etc., the triploid oyster monomer method for massively culturing such as lethality is high, poor growth; Chinese invention patent, the patent No.: 200610046697.1).In addition, cultchless oyster can also realize by first adhering to rear de-base, current industry mainly adopts corrugated plating (Guo Wen etc., cultivate method and the collector of cultchless oyster, Chinese invention patent, 201010011499.8), plastic sheet (Zeng Zhinan etc., a kind of fish-farming in shallow marine water oyster monomer process the patent No.:, Chinese invention patent, 201410176922.8) etc. the patent No.: obtain cultchless oyster.This method make use of oyster attachment after crawl formula growth characteristic, but blemish in an otherwise perfect thing be obtain the left shell of oyster seed (attachment shell) more flat, whole monomer is in comparatively flat form, make monomer whose seed be worth decrease.
Summary of the invention:
Too little for specification during current oyster monomer Seedling production, easy death, poor growth, specification loses again too greatly the bad present situation of original oyster shell form, the invention provides a kind of method utilizing oyster vertical growth characteristic to prepare cultchless oyster seed, it not only remains the original Morphology of shell of oyster, and can increase the rate of collecting seedling, improve survival rate, accelerate its growth rate.
The present invention utilizes oyster to have vertical growth characteristic under high density, takes off the links such as base obtain a large amount of healthy kind specification monomer spat of oyster kind by juvenile mollusk cultivation, juvenile mollusk.Not only remain the original Morphology of shell of oyster, increase economic worth, and have that growth is fast, survival rate is high, output advantages of higher.
The method utilizing oyster vertical growth characteristic to prepare cultchless oyster seed of the present invention, is characterized in that, comprise the following steps:
A, high density are collected seedling: occur that the oyster larva of eyespot, the overhanging state of foot is as the period of collecting seedling using 20-30%, larval density controls at 2-3/mL, utilize oyster shell to collect seedling as adherance, approximately passing through the 5-10d time completes and collects seedling, and juvenile mollusk is attached on oyster shell;
B, juvenile mollusk are cultivated: the shrimp pool water after filtering using 60-80 order is as Micro Algae feed source, and micro-air-charging incubation carries out juvenile mollusk and cultivates 30-45d, and period often changes water, and wherein, the conventional water quality index of temperature, salinity, pH is determined according to every seed oyster Biological characteristics;
C, juvenile mollusk take off base: when juvenile mollusk shell Seedling height is to 20-30mm, every a slice oyster shell has all been covered with the seed of vertical growth, has broken gently and get, and scraping can obtain monomer seed in flakes afterwards.
Described monomer seed is shell height 20-30mm, can direct more than 8mm mesh cylinder mould bag hanging culture, easy and simple to handle.
During described a step high density is collected seedling, the described oyster shell that utilizes is collected seedling as adherance, and it selects the right shell of oyster to collect seedling as adherance, and the density of oyster shell is 2000-3000 sheet/m 3, utilize gas tube to separate between every two oyster shells, form 2-3cm gap.
During described b step juvenile mollusk is cultivated, shrimp pool water refers to that transparency is 5-10cm with the seawater in the shrimp-cultivation pool pool of a large amount of Micro Algae and organic debris, and the described water that changes changes water 30-50% every 3d.
Described step c juvenile mollusk takes off in base, and carrying out the standard that juvenile mollusk takes off base is when picking up adherance in pond, when about having about 3-5% seed naturally to come off, both can carry out juvenile mollusk and take off base.
Method of the present invention is applicable to the economic kind such as Hong Kong oyster, Crassostrea rivularis, Kumamoto oyster, long oyster, Portuguese oyster and homology or allopolyploid and distant hybrid etc. thereof accordingly.
The present invention and traditionally oyster monomer seedling and seed production process are essentially different, there is following difference: 1. obtain manner is different: the oyster monomer seed utilizing chemicals norepinephrine adrenaline to produce traditionally both at home and abroad, the monomer seed that this method is produced also exists specification little (1-3mm), poor growth, the high deficiency of lethality, and cultivate more loaded down with trivial details (the Zhang Zhi bosom etc. of link, triploid oyster monomer method for massively culturing, Chinese invention patent, the patent No.: 200610046697.1); And the present invention induces without the need to chemicals, the seed shell height 20-30mm that can directly obtain, can direct more than 8mm mesh cylinder mould bag hanging culture, easy and simple to handle.2. the present invention and corrugated plating obtain method (Guo Wen etc., the method for cultivation cultchless oyster and collector, Chinese invention patent, the patent No.: 201010011499.8) although be stripping to obtain monomer, but still there is marked difference of cultchless oyster.It is make use of the feature that oyster has formula growth of crawling on corrugated plating that corrugated plating obtains cultchless oyster, and the left shell of monomer whose seed (attachment shell) is flat, and whole seed is all in comparatively flat form; And the present invention's utilization is that oyster has vertical growth characteristic under high density, obtain monomer seed and have the more three-dimensional Morphology of shell of oyster, more beautiful in appearance, economic worth is higher.3. the present invention and the different (Zeng Zhinan etc. of large gauge oyster seed that plastic sheet obtains, a kind of fish-farming in shallow marine water oyster monomer process, Chinese invention patent, the patent No.: 201410176922.8), in fact the method obtains 4-5cm seed has had half to be in attachment form, left shell is flat form, and carrying out monomer, to form the product formed in process be the oyster that one is in " half monomer half attachment " state, has form difference to a certain degree with free cultchless oyster; And present invention employs vertical growth characteristic, remain the form that oyster shell is more three-dimensional, its Morphology of shell can be made to be in nature extended configuration.
The present invention utilizes oyster juvenile mollusk (2-3/mL) under high density to have the characteristic of vertical growth, in conjunction with the phenomenon that 20-30mm juvenile mollusk tool comes off individually naturally; Collected seedling by high density, juvenile mollusk is cultivated, juvenile mollusk takes off the technological means such as base, a large amount of healthy complete medium format cultchless oyster seed (survival rate >=95%) can be obtained.The monomer seed that the present invention obtains, different from the monomer seed that formula of crawling grows, right shell is more flat that corrugated plating, plastic sheet etc. obtain, have the more three-dimensional Morphology of shell that oyster is original, its outward appearance is more beautiful, and economic worth is higher.The cultchless oyster seed that the present invention obtains, directly can utilize the bolting silk cylinder mould of more than 8mm mesh to form, avoid and utilized < 8mm mesh mesh bag intermediate rearing process, simplifies cultchless oyster and cultivates link.Cultchless oyster seed of the present invention has the advantages such as fast growth, survival rate is high, output is large.The present invention has the advantages such as easy and simple to handle, with low cost, economic and practical, and this is that the development of oyster monomer aquaculture industry provides new feasible program.
Embodiment
Following examples further illustrate of the present invention, but be not construed as limiting the invention.
Embodiment 1
A, high density are collected seedling: during the 6-8 months in 2012, and in Dalian, Zhuanghe's seashells nursery carries out oyster Seedling production.Occur that eyespot, foot Hong Kong oyster of overhanging state and long oyster hybridization triploid larva are for the period of collecting seedling with 30%, its density is 2/mL, 50 string oyster shell adherances (1000) are utilized to collect seedling as in 5 white buckets of 100L, approximately passing through the 5-10d time has collected seedling, and juvenile mollusk is attached on oyster shell.Wherein, adherance all adopts the right shell punching of oyster, utilizes plastic ties to pierce and forms, utilize 2.5cm gas tube to separate between every two panels oyster shell, form certain intervals, often string adherance 20 oyster shells.
B, juvenile mollusk are cultivated: in the ecological shrimp pond of Dalian Zhuanghe's seashells nursery, shrimp pool water is as Micro Algae feed source after 80 mesh sieve tulles filter, and every day, feeding volume was the 10-20% cultivating water body; Micro-air-charging incubation carries out juvenile mollusk and cultivates 42d, changes water 30% every 3d.During this period, shrimp pool water water temperature controls at 27.2-29.0 DEG C, salinity 24-27ppt, pH7.82-8.64, and transparency is at 3-5cm.
C, juvenile mollusk take off base: when juvenile mollusk shell Seedling height is to 20-30mm, every a slice oyster shell has all been covered with the seed of vertical growth, has taken up gently and put down, had about 3-5% seed naturally to come off; Now, bring gloves, break gently, scraping in blocks, can obtain a large amount of medium and small specification monomer seed afterwards.Through statistics, 1000 adherances obtain the high Hong Kong oyster at 20-30mm of shell altogether and long oyster hybridizes 300,000, monomer seed, and monolithic output is 300, and unit of water body output of seedling is 600,000/m 3.
This monomer seed can directly utilize the bolting silk cylinder mould of more than 8mm mesh to form, avoid and utilized < 8mm mesh mesh bag intermediate rearing process, simplify cultchless oyster and cultivate link, this cultchless oyster seed ratio traditionally drug treating cultchless oyster growth rate can improve 10-20%, survival rate improves 15-30%, output increased 30-50%.
Embodiment 2
A, high density are collected seedling: during the 6-9 months in 2013, carry out oyster Seedling production in marine economic animal experiment station, Zhanjiang of profound Chinese Academy of Science Nanhai Ocean Research Institute.Occur that Hong Kong oyster larva of eyespot, the overhanging state of foot is as material using 25%, its density is 2.5/mL, utilizes 1000 string oyster shell adherances (20000) as 10m 3collect seedling in cement pit, approximately passing through the 5-10d time has collected seedling, and juvenile mollusk is attached on oyster shell.Wherein, adherance all adopts the right shell punching of oyster, utilizes plastic ties to pierce and forms, utilize 2.5cm oxygen hose to separate between every two panels oyster shell, form certain intervals, often string adherance 20 oyster shells.
B, juvenile mollusk are cultivated: using the shrimp pool water of artificial culture after 80 mesh sieve tulles filter as Micro Algae feed source, every day, feeding volume was cultivate the 10-30% of water body; Micro-air-charging incubation carries out juvenile mollusk and cultivates 45d, changes water 50% every 3d.During this period, shrimp pool water water temperature controls at 28.0-29.6 DEG C, salinity 20-25ppt, pH7.75-8.56, and transparency is at 3-5cm.
C, juvenile mollusk take off base: when juvenile mollusk shell Seedling height is to 20-30mm, every a slice oyster shell has all been covered with the seed of vertical growth, has taken up gently and put down, had about 3-4% seed naturally to come off; Now, bring gloves, break gently, scraping can obtain a large amount of medium and small specification monomer seed in flakes afterwards.Through statistics, 20000 adherances obtain the high Hong Kong oyster at 20-30mm of shell altogether and long oyster hybridizes 6,400,000, monomer seed, and monolithic output is 320, and unit of water body output of seedling is 640,000/m 3.
This monomer seed can directly utilize the bolting silk cylinder mould of more than 8mm mesh to form, avoid and utilized < 8mm mesh mesh bag intermediate rearing process, simplify cultchless oyster and cultivate link, this cultchless oyster seed ratio traditionally drug treating cultchless oyster growth rate can improve 15-30%, survival rate improves 20-30%, output increased 30-60%.
Embodiment 3
A, high density are collected seedling: during the 6-9 months in 2013, carry out oyster Seedling production at shellfish complex experiment station, Guangxi.Occur that the Kumamoto oyster larva of eyespot, the overhanging state of foot is as material using 30%, its density is 3/mL, utilizes 1000 string oyster shell adherances (20000) as 10m 3collect seedling in cement pit, approximately passing through the 5-10d time has collected seedling, and juvenile mollusk is attached on oyster shell.Wherein, adherance all adopts the right shell punching of oyster, utilizes plastic ties to pierce and forms, utilize 2.5cm oxygen hose to separate between every two panels oyster shell, form certain intervals, often string adherance 20 oyster shells.
B, juvenile mollusk are cultivated: using the seawater in ecological shrimp pond after 60 mesh sieve tulles filter as Micro Algae feed source, every day, feeding volume was cultivate the 10-30% of water body; Micro-air-charging incubation carries out juvenile mollusk and cultivates 30d, changes water 50% every 3d.During this period, shrimp pool water water temperature controls at 27.9-29.5 DEG C, salinity 18-24ppt, pH 7.83-8.67, and transparency is at 3-5cm.
C, juvenile mollusk take off base: when juvenile mollusk shell Seedling height is to 20-30mm, every a slice oyster shell has all been covered with the seed of vertical growth, has taken up gently and put down, had about 3-4% seed naturally to come off; Now, bring gloves, break gently, scraping can obtain a large amount of medium and small specification monomer seed in flakes afterwards.Through statistics, 20000 adherances obtain the high Hong Kong oyster at 20-30mm of shell altogether and long oyster hybridizes 7,200,000, monomer seed, and monolithic output is 360, and unit of water body output of seedling is 720,000/m 3.
This monomer seed can directly utilize the bolting silk cylinder mould of more than 8mm mesh to form, avoid and utilized < 8mm mesh mesh bag intermediate rearing process, simplify cultchless oyster and cultivate link, this cultchless oyster seed ratio traditionally drug treating cultchless oyster growth rate can improve 20-30%, survival rate improves 15-30%, output increased 40-60%.

Claims (4)

1. utilize oyster vertical growth characteristic to prepare a method for cultchless oyster seed, it is characterized in that, comprise the following steps:
A, high density are collected seedling: occur that the oyster larva of eyespot, the overhanging state of foot is for the period of collecting seedling with 20-30%, larval density controls at 2-3/mL, and utilize oyster shell to collect seedling as adherance, complete through the 5-10d time and collect seedling, juvenile mollusk is attached on oyster shell;
B, juvenile mollusk are cultivated: the shrimp pool water after filtering using 60-80 order is as Micro Algae feed source, and micro-air-charging incubation carries out juvenile mollusk and cultivates 30-45d, and period often changes water, and wherein, the conventional water quality index of temperature, salinity, pH is determined according to every seed oyster Biological characteristics;
C, juvenile mollusk take off base: when juvenile mollusk shell Seedling height is to 20-30mm, every a slice oyster shell has all been covered with the seed of vertical growth, has broken gently and get, and scraping can obtain monomer seed in flakes afterwards.
2. method according to claim 1, is characterized in that, during described a step high density is collected seedling, the described oyster shell that utilizes is collected seedling as adherance, and it selects the right shell of oyster to collect seedling as adherance, and the density of oyster shell is 2000-3000 sheet/m 3, separate with plastics tracheae between every two oyster shells, form 2-3cm spacing.
3. method according to claim 1, is characterized in that, during described b step juvenile mollusk is cultivated, shrimp pool water refers to that transparency is 5-10cm with the seawater in the shrimp-cultivation pool pool of a large amount of Micro Algae and organic debris, and the described water that changes changes water 30-50% every 3d.
4. method according to claim 1, is characterized in that, described step c juvenile mollusk takes off in base, and the standard of carrying out is when picking up adherance in pond, when about having about 3-5% seed naturally to come off, namely can carry out juvenile mollusk and take off base.
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CN104855307A (en) * 2015-04-22 2015-08-26 中国科学院南海海洋研究所 Non-damage preparation method for single crassostrea hongkongensis
CN106135079A (en) * 2016-06-24 2016-11-23 广西壮族自治区水产科学研究院 A kind of method of cultchless oyster pond intermediate rearing
CN106942105A (en) * 2017-05-11 2017-07-14 中国海洋大学 A kind of method for carrying out Hong Kong oyster artificial breeding using the northern megathermal period
CN108293927A (en) * 2017-12-19 2018-07-20 广西壮族自治区水产科学研究院 A method of it improving cultchless oyster and takes off base survival rate
CN111034662A (en) * 2019-12-31 2020-04-21 广西壮族自治区水产科学研究院 Method for obtaining hong Kong oyster monomer by drug induction

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Publication number Priority date Publication date Assignee Title
CN104855307A (en) * 2015-04-22 2015-08-26 中国科学院南海海洋研究所 Non-damage preparation method for single crassostrea hongkongensis
CN104855307B (en) * 2015-04-22 2017-03-15 中国科学院南海海洋研究所 A kind of not damaged preparation method of Hong Kong oyster monomer
CN106135079A (en) * 2016-06-24 2016-11-23 广西壮族自治区水产科学研究院 A kind of method of cultchless oyster pond intermediate rearing
CN106942105A (en) * 2017-05-11 2017-07-14 中国海洋大学 A kind of method for carrying out Hong Kong oyster artificial breeding using the northern megathermal period
CN106942105B (en) * 2017-05-11 2020-03-17 中国海洋大学 Method for artificially breeding seedlings of crassostrea hongkongensis by utilizing northern high-temperature period
CN108293927A (en) * 2017-12-19 2018-07-20 广西壮族自治区水产科学研究院 A method of it improving cultchless oyster and takes off base survival rate
CN111034662A (en) * 2019-12-31 2020-04-21 广西壮族自治区水产科学研究院 Method for obtaining hong Kong oyster monomer by drug induction

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