CN105360022B - A kind of producing method for seed of double golden shell Kumamoto oyster strains - Google Patents
A kind of producing method for seed of double golden shell Kumamoto oyster strains Download PDFInfo
- Publication number
- CN105360022B CN105360022B CN201510670751.9A CN201510670751A CN105360022B CN 105360022 B CN105360022 B CN 105360022B CN 201510670751 A CN201510670751 A CN 201510670751A CN 105360022 B CN105360022 B CN 105360022B
- Authority
- CN
- China
- Prior art keywords
- oyster
- shell
- golden shell
- kumamoto
- kumamoto oyster
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of producing method for seed of double golden shell Kumamoto oyster strains.The present invention utilizes the golden characteristic with superdominance expression in Kumamoto oyster bivalve, with reference to golden shell color and bata-carotene content positive correlation principle, by key foundation group structure, the orientation purifying of double golden shell mass screenings, double golden shell strains, so as to obtain 100% pair of golden shell Kumamoto oyster strain.Double golden shell Kumamoto oyster strains that the present invention obtains, there is stable heredity, outward appearance is golden yellow, commodity value is high.The present invention imports without foreign gene, has the advantages that safety nuisance free, the especially more common oyster of bata-carotene content improves 1.8 3.0 times.The present invention provides approach for Kumamoto oyster rearing new variety, and new breeding is also provided for Kumamoto oyster aquaculture.
Description
Technical field：
The invention belongs to shellfish genetic breeding field in marine biotechnology, and in particular to a kind of double golden shell Kumamoto oyster product
The producing method for seed of system.
Background technology
Kumamoto oyster Crassostrea sikamea originate in Southeast Asia, including the ground such as China, South Korea, Japan.It likes
Inclined salt water marine site in brackish water region is lived in, is distributed on the south China the Changjiang river (including Nantong), such as Zhejiang, Fujian, wide
The ground such as east, Guangxi, Hainan.Although smaller, the delicious flavour of Kumamoto oyster individual, in the U.S., its single live body price is higher,
For 2 times or so of long oyster, American oyster.In China, Kumamoto oyster is known as oyster oyster, yellow oyster, iron fourth oyster etc., its fresh meat price again
It is that head is minimum in the huge oyster category in China for 1.5-2.0 times of Hong Kong oyster, 2-3 times of long oyster, 3-4 times of Portuguese oyster,
But most there is a seed oyster of business development potentiality.
At present, the sub- ox shellfish of Chinese Academy of Science Nanhai Ocean Research Institute of China analogy breeds and genetic breeding team has broken through
The extensive artificial breeding technique of Kumamoto oyster, has cultivated the more ridge strain F3s of fast long Kumamoto oyster.Meanwhile introduce beautiful
State's Kumamoto oyster, 3 key foundation colonies are constructed, abundant material is provided for its breeding.Inventor team was in recent years
Research in find, Kumamoto oyster is there is abundant shell chromatic variation, and left and right shell color genetic mechanism is different, and this is shell chromaticity system
Research and development provide possibility.
The content of the invention：
In order to solve the problems, such as do not have new quality variety in current Kumamoto oyster industry, the invention provides a kind of double golden shells
The producing method for seed of Kumamoto oyster strain, the golden shell that outward appearance is beautiful, shell mould is neat can be not only cultivated using the producing method for seed
Chromaticity system, and provide opportunity for its rearing new variety.
The producing method for seed of double golden shell Kumamoto oyster strains of the present invention, it is characterised in that comprise the following steps：
D, key foundation group builds：Using wild Kumamoto oyster as parent, filial generation is bred by the way of numerous using colony and entered
Row key foundation group builds；
E, double golden shell mass screenings：When the filial generation Kumamoto oyster in step a grows to shell high 10-20mm stages, screening
Bivalve is golden individual, is formed using conventional monomeric aquaculture model, when sexual gland maturation, by determining in oyster outer embrane
Content beta-carotene, filter out the individual of content highest 10% and be used as parental population, breed pair golden shell first filial generations, afterwards according to
Conventional monomeric aquaculture model is formed；
F, double golden shell strain purifying：Using double golden shell first filial generations individual in step b as material, by the high 10-20mm of shell
The double golden shell individuals of juvenile mollusk stage postsearch screening are individually formed, and when sexual gland maturation, determine beta carotene in adult outer embrane again
Content, filter out the individual of content beta-carotene highest 10% and be used as reproductive population, second filial is carried out from numerous, according to oyster list
Body conventional cultivation pattern is formed, that is, obtains stable heredity, and bivalve is 100% golden double golden shell Kumamoto oyster strains.
In described key foundation group structure, wild Kumamoto oyster parent quantity is preferably selected more than 5000, parent
It is to prevent inbreeding depression phenomenon generation from now on mostly.
In described double golden shell mass screenings, employ juvenile mollusk stage golden shell color and screen and form for the first time rear beta carotene
The postsearch screening of content, realize the accurate selection of golden shell color individual.
It is because oyster is cultivating why using juvenile mollusk stage screening golden shell color in described double golden shell mass screenings
During, color shades can be influenceed by environment, and especially brackish water type oyster shell color is easy to be corroded, therefore is formed at it
Afterwards, accurately selected as correlation metric using content beta-carotene.
In described double golden shell strains purifying, taking full advantage of the double golden shells of Kumamoto oyster has the characteristics of epistatic effect, only
Need continuous two generations double standards to select, can both obtain 100% pair of golden shell filial generation of stable heredity.
The present invention is different from documents 1 (Zeng Zhinan, Ning Yue, Qi Jianfei, Wu Qisheng, a kind of shell golden yellow Su Chang Portugals
The breeding method of grape tooth oyster new lines, national inventing patent, the patent No.：201410197671.1) and documents 2 (Li Qi,
Kong Lingfeng, Wang Weijun, Cong Hao, Ge Jianlong, Wang Xubo, a kind of selection of the long oyster new lines of shell gold, country's invention are special
Profit, application number：201410120162.9).Compared with the present invention, the innovative point of documents 1 is with golden shell color Portuguese oyster
As experiment material, enter scanning frequency length system and cultivate, and first point is how to cultivate double golden shell Kumamoto oyster product to the main wound of the present invention
System, non-growth traits selection；Documents 2 mainly utilize conventional breeding means, cultivate the long oyster of golden shell color, its selection refers to
Mark is excessively single, and sole selection criteria be used as just with gold, and the present invention is in order to select golden shell chromaticity system basic
On, using content beta-carotene and golden shell color positive correlation principle, further screen the high parent of content beta-carotene and carried out
Precisely purifying, accelerate golden shell chromaticity system breeding process.Documents 1 and 2 cultivate the Portuguese oyster of golden shell color and long oyster
It was required to for 4 generations, and the present invention needed only to for 2 generations and can both cultivate 100% pair of golden shell Kumamoto oyster strain of stable heredity, greatly
Shorten breeding process greatly.As can be seen here, although documents 1 and 2 are golden shell color oyster strain developments, with the present invention
Breeding method and its selection approach it is entirely different, any destruction not yet is caused to originality of the present invention.In addition, documents 1
Be long oyster and Portuguese oyster with 2 research objects, both oysters are high salt species, its shell color from juvenile mollusk until to
Adult expression is highly stable, and the Kumamoto oyster of the present invention is a kind of brackish water type oyster, and its shell color table reaches very bright in early days
It is aobvious, but after adult, shell color is often corroded by brackish water, so, shell color selection standard of the present invention is young in 10-20mm
Shellfish, the problem that can not be confirmed after being corroded into body shell color is effectively avoided.In order to further improve golden shell chroma color uniformity,
The present invention is selected with content beta-carotene in outer embrane and golden shell color positive correlation principle first, greatly strengthen golden shell
The regularity of body colour degree.
The present invention is using the golden characteristic with superdominance expression in Kumamoto oyster bivalve, with reference to golden shell color and beta carotene
Content positive correlation principle, by key foundation group structure, the orientation purifying of double golden shell mass screenings, double golden shell strains, so as to obtain
100% pair of golden shell Kumamoto oyster strain, accelerates Kumamoto oyster breeding process, and theoretical foundation and reality are provided as kind for it
Trample experience.Double golden shell Kumamoto oyster strains that the present invention obtains, there is stable heredity, outward appearance is golden yellow, commodity value is high.
The present invention imports without foreign gene, has the advantages that the more common oyster of safety nuisance free, especially content beta-carotene improves
1.8-3.0 again.The present invention provides approach for Kumamoto oyster rearing new variety, also provides newly good for Kumamoto oyster aquaculture
Kind.
Embodiment：
Following examples are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1
A, key foundation group builds：In in late May, 2013, passed through in Chinese Academy of Science Nanhai Ocean Research Institute Zhanjiang ocean
Help animal experiment station group based on the wild individual of Kumamoto oyster 6230 in Zhanjiang of maturation, and by drying in the shade, stimulation by running water uses group
Body carries out key foundation group's structure from numerous mode, produces D-shaped larva 1.2 hundred million altogether, is respectively placed in 3 20m3Cement pit
In, according to the South China coastal salinity gradual change type Kumamoto oyster breeding method culture, it is specially：By normotrophic D-shaped larva, use
The mode of micro- inflation carries out larvae cultivation in 20~25ppt of salinity under high salinity seawater, during which, does not change water, bait is with green
Based on Se Bafu algaes, yeast is equipped with, is fed using a small amount of repeatedly mode；When it eyespot, foot overhanging stage occurs, using going
Methylepinephrine handles eyebot larvae, and monomer cultivation is carried out using sinking device；Juvenile mollusk is bred as under middle salt environment：Juvenile mollusk is placed in
Juvenile mollusk incubation is carried out in 15~20ppt of salinity middle salinity seawater, is during which fed using shrimp pool water as biological feed, is kept
Water is constantly in pico- green tinged state, through cultivating the Kumamoto oyster seed that can secure good health.
B, double golden shell mass screenings：Cultivated by 54d, the high 10-20mm monomer seeds 3,620,000 of shell are obtained, by careful
Select, filter out double golden shell juvenile mollusks 12000.Afterwards, by its using scallop cage hanging culture in Zhanjiang East Sea Island island in Guangdong Province water channel, entirely
In breeding process, periodic cleaning sludge, fouling organism etc., large mesh cylinder mould is changed.Cultivated by the anniversary, obtain underlying group
Double golden shells individual of 9360 high 30-50mm of shell in body.In in late May, 2014,1500 oyster sexual glands of random screening into
Ripe double golden shells individual, is placed in 200L Plastic Drums, adds 3% MgCl2Solution, 2-3h is approximately passed through, it is all male
Oyster is anesthetized, and 3-5mg outer embranes are gently taken out using tweezers, utilizes β-carrot in Elisa kit measurement oyster outer embranes
Cellulose content, filter out 136 individuals of content highest 10% as parental population (because there is 140 individual bivalves to close, nothing
Method obtains sample, altogether sample size 1360), wherein, female is 113, and male is 23, using the method for mixing smart ovum
D-shaped larva 12,000,000 is obtained, is placed on 4m2Cultivated in cement, when it eyespot, foot overhanging stage occurs, using going first
Adrenaline handles eyebot larvae, and monomer cultivation is carried out using sinking device.
C, double golden shell strain purifying：Cultivated by 60d, obtain the high 12-18mm monomer seeds 750,000 of shell, 100% is
Golden yellow individual, but in order to which golden colourity is further purified, randomly selected the golden yellow juvenile mollusk 9600 of colourity.Afterwards, will
It utilizes scallop cage hanging culture in Zhanjiang East Sea Island island in Guangdong Province water channel, whole breeding process, periodic cleaning sludge, fouling organism etc.,
Change large mesh cylinder mould.Cultivated by the anniversary, obtain double golden shells of 7536 high 30-50mm of shell in basic population
Body.In in late May, 2015, the consistent golden yellow individual of 1800 ripe colourities of random screening oyster sexual gland, is put again
It is placed in 200L Plastic Drums, adds 3% MgCl2Sea water solution, 2-3h is approximately passed through, all oysters are anesthetized, and utilize tweezer
Son gently takes out 3-5mg outer embranes, using content beta-carotene in Elisa kit measurement oyster outer embranes, filters out content
154 individuals of highest 10% (because there is 260 individual bivalves to close, can not obtain sample, altogether sample as parental population
Product weight 1540), wherein, female is 121, and male is 33, and D-shaped larva 15,000,000 is obtained using the method for mixing smart ovum
It is individual, it is placed on 5m2Cultivated in cement, when it eyespot, foot overhanging stage occurs, it is young to handle eyespot using norepinephrine
Worm, monomer cultivation is carried out using sinking device.Cultivated by 63d, obtain 1,200,000 15-20mm juvenile mollusks altogether, bivalve is
Golden yellow, and colourity is consistent, thus obtains stable heredity, bivalve is 100% golden double golden shell Kumamoto oyster strains.This pair
The more common Kumamoto oyster of content beta-carotene of golden shell Kumamoto oyster strain improves 1.8-3.0 times
The follow-up strain finds that its filial generation is 100% golden Kumamoto oyster all for bivalve by some Dai Zifan, by
, it has been confirmed that the invention described above has obtained stable heredity, bivalve is 100% golden Kumamoto oyster strain for this.
The specific embodiment of invention is above are only, but the design concept of the present invention is not limited thereto, it is all to utilize design pair
The present invention carries out the change of unsubstantiality, all should belong to the behavior for invading the scope of the present invention.
Claims (2)
1. a kind of producing method for seed of double golden shell Kumamoto oyster strains, it is characterised in that comprise the following steps：
A, key foundation group builds：Using wild Kumamoto oyster as parent, filial generation is bred by the way of numerous using colony and carries out core
Heart underlying group is built；
B, double golden shell mass screenings：When the filial generation Kumamoto oyster in step a grows to shell high 10-20mm stages, bivalve is screened
It is golden individual, is formed using conventional monomeric aquaculture model, when sexual gland maturation, by determining β-Hu in oyster outer embrane
Radish cellulose content, filter out the individual of content highest 10% and be used as parental population, double golden shell first filial generations are bred, afterwards according to routine
Monomer aquaculture model is formed；
C, double golden shell strain purifying：Using double golden shell first filial generations individual in step b as material, by the high 10-20mm juvenile mollusks of shell
The double golden shell individuals of stage postsearch screening are individually formed, and when sexual gland maturation, are determined beta carotene in adult outer embrane again and are contained
Amount, filter out the individual of content beta-carotene highest 10% and be used as reproductive population, progress second filial is from numerous, according to oyster monomer
Conventional cultivation pattern is formed, that is, obtains stable heredity, and bivalve is 100% golden double golden shell Kumamoto oyster strains.
2. the producing method for seed of double golden shell Kumamoto oyster strains according to claim 1, it is characterised in that described core base
In plinth group structure, wild Kumamoto oyster parent quantity is more than 5000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670751.9A CN105360022B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double golden shell Kumamoto oyster strains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670751.9A CN105360022B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double golden shell Kumamoto oyster strains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105360022A CN105360022A (en) | 2016-03-02 |
| CN105360022B true CN105360022B (en) | 2017-11-21 |
Family
ID=55363119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510670751.9A Active CN105360022B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double golden shell Kumamoto oyster strains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105360022B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699541A (en) * | 2019-02-19 | 2019-05-03 | 中国科学院南海海洋研究所 | A kind of producing method for seed of bluish violet outer embrane sarranine giant clam strain |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950448A (en) * | 2017-12-07 | 2018-04-24 | 重庆凌丰科技有限公司 | A kind of producing method for seed of double purple black long oyster strains of shell |
| CN113693000A (en) * | 2021-09-23 | 2021-11-26 | 广东海洋大学 | Production method of steel gray pearls |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133725A (en) * | 2007-09-30 | 2008-03-05 | 中国水产科学研究院黄海水产研究所 | Cultivating method of the novel strain of white patinopecten yessoensis |
| CN101855999A (en) * | 2009-04-10 | 2010-10-13 | 中国科学院海洋研究所 | Method for selectively breeding bivalve shellfish |
| CN101990854A (en) * | 2010-10-29 | 2011-03-30 | 中国海洋大学 | Breeding method of pure color breeding line of pacific oyster shells |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
| CN103975879A (en) * | 2014-05-12 | 2014-08-13 | 福建省水产研究所 | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006061137A (en) * | 2004-08-26 | 2006-03-09 | Yoshinobu Haraguchi | Method for preparing multicolor pearl |
-
2015
- 2015-10-13 CN CN201510670751.9A patent/CN105360022B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133725A (en) * | 2007-09-30 | 2008-03-05 | 中国水产科学研究院黄海水产研究所 | Cultivating method of the novel strain of white patinopecten yessoensis |
| CN101855999A (en) * | 2009-04-10 | 2010-10-13 | 中国科学院海洋研究所 | Method for selectively breeding bivalve shellfish |
| CN101990854A (en) * | 2010-10-29 | 2011-03-30 | 中国海洋大学 | Breeding method of pure color breeding line of pacific oyster shells |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
| CN103975879A (en) * | 2014-05-12 | 2014-08-13 | 福建省水产研究所 | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells |
Non-Patent Citations (1)
| Title |
|---|
| 文蛤（Meretrix meretrix）壳色花纹性状的分子遗传基础研究;齐晓艳;《中国优秀硕士学位论文全文数据库 农业科技辑》;20150315(第3期);第D052-113页 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699541A (en) * | 2019-02-19 | 2019-05-03 | 中国科学院南海海洋研究所 | A kind of producing method for seed of bluish violet outer embrane sarranine giant clam strain |
| CN109699541B (en) * | 2019-02-19 | 2020-11-10 | 中国科学院南海海洋研究所 | Seed production method of blue-purple mantle safranin tridacna strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105360022A (en) | 2016-03-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102499130B (en) | Breeding method of low-salt-resistant line of blue crab | |
| Nair et al. | Current status and prospects of farming the giant river prawn M acrobrachium rosenbergii (D e M an) and the monsoon river prawn M acrobrachium malcolmsonii (HM E dwards) in I ndia | |
| CN105165686B (en) | A kind of producing method for seed of double black shell Kumamoto oyster strains | |
| WO2017156928A1 (en) | Method for use in red spot grouper fry breeding | |
| CN103798167B (en) | The artificial breeding method of a kind of Ostrea cucullata | |
| CN105494177B (en) | A kind of producing method for seed of the long breeding line of Portuguese oyster speed | |
| WO2015021785A1 (en) | Method for breeding new-variety crassostrea gigas with orange left and right shells | |
| CN103858803A (en) | Method for breeding new golden-shell crassostrea gigas strain | |
| CN103975879A (en) | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells | |
| CN103891647B (en) | The producing method for seed of the resistance to high salt new lines of a kind of Hong Kong oyster | |
| CN105165685B (en) | A kind of producing method for seed of double blank shell Kumamoto oyster strain | |
| CN105360022B (en) | A kind of producing method for seed of double golden shell Kumamoto oyster strains | |
| CN103478028A (en) | Directional cultivation method of white or purple fresh water pearl | |
| CN105918168B (en) | Exopalaemon carinicauda saline-alkali water artificial cultivating method | |
| CN101884311B (en) | Method for constructing tongue sole families and breeding superior families | |
| Padmakumar et al. | Breeding of endemic catfish, Horabagrus brachysoma in captive conditions | |
| CN104304104B (en) | Method for preparing monomer oyster fries by utilizing vertical growth characteristics of oysters | |
| CN105918185A (en) | Method for artificial breeding of tridacnidae | |
| CN103766261B (en) | Exopalaemon carinicauda 1 year continuous many batches of artificial cultivating method | |
| CN105393958A (en) | Current year fish fingerling indoor cement pool high density aquaculture method for bigmouth grenadier anchovy artificial breeding | |
| CN102884995A (en) | Desalination cultivation method for green mud crab early larvae | |
| CN106135079A (en) | A kind of method of cultchless oyster pond intermediate rearing | |
| CN101861839B (en) | Method for artificially breeding purple nacre strain hyriopsis cumingii | |
| Li et al. | Freshwater pearl culture | |
| CN103960181B (en) | A kind of method of dace and the pond culture of caridina nilotica gracilipes supporting supply cutter long-tailed anchovy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |