CN103891647A - Breeding method of new halophilic crassostrea hongkongensis variety - Google Patents
Breeding method of new halophilic crassostrea hongkongensis variety Download PDFInfo
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- CN103891647A CN103891647A CN201410117201.XA CN201410117201A CN103891647A CN 103891647 A CN103891647 A CN 103891647A CN 201410117201 A CN201410117201 A CN 201410117201A CN 103891647 A CN103891647 A CN 103891647A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a breeding method of a new halophilic crassostrea hongkongensis variety. The breeding method includes steps of a, selection of parents: utilizing wild crassostrea hongkongensis of sea areas with average salinity>=24ppt in a coastal region of South China as a halophilic parent group; b, promoting mature of the parents: utilizing the parent group in the step a as a basic group, and disposing the parent group to promote mature in sea water with 27-30ppt of salinity; c, breeding of offspring: utilizing the mature parent group in the step b as a breeding group, breeding the offspring in the sea water with 27-30ppt of salinity by a conventional method and obtaining a new halophilic variety F1; d, purely breeding the variety: utilizing the halophilic variety F1 in the step c as the parents, repeating b-c steps several times, and obtaining the new halophilic crassostrea hongkongensis variety via several generations of continuous pure breeding. The new halophilic crossostrea hongkongensis variety can be bred in the pure sea water, the convention that oysters can only be bred around estuary areas is finished, breeding area is enlarged by multiples, yield and output value are greatly improved.
Description
Technical field:
The invention belongs to shellfish genetic breeding field in modern marine agricultural, be specifically related to the producing method for seed of the resistance to high salt new lines of a kind of Hong Kong oyster.
Background technology:
Hong Kong oyster Crassostrea hongkongensis is the Main Economic kind of China's the South China coastal cultivation, like living in nearly river mouth or near have fresh water to inject place, mainly be distributed in the ground such as Guangdong, Guangxi, annual production is at more than 130 ten thousand tons, the output value is in hundred million yuan of 80-100 (China Fisheries yearbook, 2013).Hong Kong oyster is compared with other oyster kind, its value is mainly reflected in body pork fertilizer, delicious flavour, its market value, far above other kind oyster, is subject to Guangdong, Guangxi, Hongkong and Macro, some national consumer's favors (Zhang Yuehuan etc., 2014) of Southeast Asia deeply in addition.
Hong Kong oyster has the cultivation history of nearly one thousand years in areas such as China Guangdong, Guangxi, Fujian, Hongkong and Macro.Regrettably, scholars not yet cultivate Hong Kong oyster improved Varieties so far.
Summary of the invention:
The object of the invention is, for the present situation that there is no resistance to high salt new varieties in current Hong Kong oyster culture industry, provides the producing method for seed of the resistance to high salt new lines of a kind of Hong Kong oyster.Can obtain the resistance to high salt new lines of Hong Kong oyster by the method, it can form in full sea water, the custom tightly cultivating around estuary region is traditionally through with, make its cultured area expand several times, output and the output value have obtained increasing substantially, for solid foundation has been established in oyster industry fast steady sustainable development in Hong Kong.
For aquatic livestock, scholars successively cultivate the new lines such as the imitative stichopus japonicus (Chang Yaqing etc., 2009) of resistance to less salt, Portunus trituberculatus Miers (Li Jian etc., 2011), Porphyra haitanensis (Yan Xinghong etc., 2013), but there is not yet the relevant report about resistance to high salt strain.Analyzing its main cause is that self all lives in high salinity region because aquatic livestock is high salt region stenohalinity kind in most of seawater.For the kind of euryhalinous Hekou District, cultivating resistance to high salt new lines can increase culturing area, improves output, also may promote its product quality.Because oyster culture region, Hong Kong is only distributed near estuary region traditionally, the restriction that this is serious scale and the output of this oyster culture industry.Can so, cultivate resistance to high salt new lines, thereby its effluent port area cultivation is transferred to off-lying sea, expand cultured area, improve cultured output, the quick stable development of promotion industry becomes main innovate point of the present invention.
The producing method for seed of the resistance to high salt new lines of Hong Kong of the present invention oyster, is characterized in that, comprises the following steps:
A, parent select: using wild Hong Kong oyster in average salinity >=24ppt of the South China coastal anniversary marine site as resistance to high salt parent grouping;
B, parent's accelerating: using parent grouping in a step as basic population, be positioned over accelerating in salinity 27-30ppt seawater;
C, filial generation cultivate: using parent grouping ripe in b step as reproductive population, in salinity 27-30ppt seawater, cultivate filial generation according to conventional method, obtain resistance to high salt new lines F1;
D, strain purifying: using the resistance to high salt new lines F1 of c step as parent, repeat b-c step several times, by some generations continuous purification, can obtain the resistance to high salt new lines of Hong Kong oyster.
The several times of described steps d are preferably 1-2 time, and described some generations are preferably 2-3 generation.
Wild Hong Kong oyster during described a step parent selects, is mainly derived from depth of water 5-10m, 2-3 oyster in age colony near region, off-lying sea.
Accelerating in seawater in described b step parent accelerating can be artificial ripening in ecological shrimp pond or indoor cement pit.
Conventional method in described c step filial generation cultivation is cultivated filial generation, can adopt colony, from numerous or single, single family form is obtained to filial generation.
Conventional method in described c step filial generation cultivation is cultivated filial generation, comprises the intermediate links such as fertilization and hatching, larvae cultivation, juvenile mollusk are bred as, young shellfish is formed.
The present invention adapts to the wide biological property of salt scope in conjunction with Hong Kong oyster, adopts the technological means such as parent's selection, parent's accelerating, filial generation cultivation, strain purifying; Coerce and carry out artificial selection by extreme hypersaline environment, accelerated high salt tolerance related gene purifying speed, thereby obtain the resistance to high salt new lines of Hong Kong oyster.The present invention obtains the resistance to high salt new lines of Hong Kong oyster and can in full sea water, form, the custom tightly cultivating around estuary region is traditionally through with, make its cultured area expand several times, output and the output value have obtained increasing substantially, for solid foundation has been established in oyster industry fast steady sustainable development in Hong Kong.That the present invention has is easy and simple to handle, practical, be easy to the advantages such as popularization.
Embodiment:
Further illustrate the present invention take the producing method for seed of the resistance to high salt new lines of Hong Kong oyster as embodiment, but be not construed as limiting the invention.
Embodiment 1
A, parent select: on July 5th, 2010, employ diver annual salinity >=24ppt in bay (depth of water 5-10m) behind Hong Kong to adopt in marine site to catch 200 of wild Hong Kong oysters in 2-3 age, as resistance to high salt parent grouping.
B, parent's accelerating: 200 individualities are packed in 10 scallop cages, be positioned in salinity 27-30ppt pond and carry out ecological accelerating.Through 15d accelerating, 162 Hong Kong oyster gonad maturities, discard 38 of residues dead individual.
C, filial generation are bred: using 162 Hong Kong oysters as reproductive population, in salinity 27-30ppt seawater, carry out colony from numerous, adopt anatomy mode, form through fertilization and hatching, larvae cultivation, juvenile mollusk incubation, young shellfish, obtain resistance to high salt new lines F1.Concrete growth, survival condition are as table 1, shown in table 2.
D, strain purifying: using resistance to high salt new lines F1 as parent, in 21 days July in 2011, repeat b-c step, cultivate resistance to high salt strain F2; In 22 days July in 2012, repeat b-c step, cultivate resistance to high salt strain F3; By 3 generation continuous purification, obtained the resistance to high salt new lines of Hong Kong oyster.
In above link, from resistance to high salt strain viability index (table 1), fertilization rate, incubation rate, distortion ratio, survival rate all along with algebraically increase and progressively increase.Wherein, distortion ratio changes the most remarkable, because can larva abnormal directly affect filial generation quantity, is also the bottleneck of this seed of restriction commodity production.From its growing power (table 2), under hypersaline environment, ovum footpath, D shape larva size almost not yet change; But growth rate is accompanied by algebraically, increase increases gradually, and this is a kind of result that adapts to hypersaline environment.
In a word, coerce, tame through the continuous hypersaline environment of three generations, obtain stable, the growth rate of viability faster, the resistance to high salt new lines that can carry out the production of business seed and cultivation, for oyster culture region, Hong Kong expands, output promotes, and promotes Hong Kong oyster industry to advance steadily fast and has established solid practical basis.
Table 1: resistance to high salt strain viability index comparison
| Classification | Fertilization rate (%) | Incubation rate (%) | Distortion ratio (%) | 360 age in days survival rates (%) |
| First filial generation F1 | 75.87 | 63.29 | 6.25 | 50.75 |
| Second filial F2 | 89.63 | 82.12 | 30.57 | 75.63 |
| F3 F3 | 97.56 | 95.34 | 72.58 | 91.24 |
The comparison of the resistance to high salt strain growing power index of table 2
| Classification | (μ m) in ovum footpath | (μ m) for D shape larva | 360 age in days shells high (mm) | 360 age in days fresh weights (g) |
| First filial generation F1 | 40.25 | 60.12 | 52.78 | 15.28 |
| Second filial F2 | 40.63 | 60.89 | 56.23 | 20.75 |
| F3 F3 | 40.98 | 61.37 | 61.35 | 24.59 |
Claims (5)
1. a producing method for seed for the resistance to high salt new lines of Hong Kong oyster, is characterized in that, comprises the following steps:
A, parent select: using wild Hong Kong oyster in average salinity >=24ppt of the South China coastal anniversary marine site as resistance to high salt parent grouping;
B, parent's accelerating: using parent grouping in a step as basic population, be positioned over accelerating in salinity 27-30ppt seawater;
C, filial generation cultivate: using parent grouping ripe in b step as reproductive population, in salinity 27-30ppt seawater, cultivate filial generation according to conventional method, obtain resistance to high salt new lines F1;
D, strain purifying: using the resistance to high salt new lines F1 of c step as parent, repeat b-c step several times, by some generations continuous purification, can obtain the resistance to high salt new lines of Hong Kong oyster.
2. producing method for seed according to claim 1, is characterized in that, the wild Hong Kong oyster during described a step parent selects is 2-3 oyster in the age colony that derives from depth of water 5-10m, region, close off-lying sea.
3. producing method for seed according to claim 1, is characterized in that, in the seawater in described b step parent accelerating, accelerating is artificial ripening in ecological shrimp pond or indoor cement pit.
4. producing method for seed according to claim 1, is characterized in that, the conventional method in described c step filial generation cultivation is cultivated filial generation, can adopt colony, from numerous or single, single family form is obtained to filial generation.
5. producing method for seed according to claim 1, is characterized in that, the several times of described steps d are 1-2 time, and described is some on behalf of 2-3 generation.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381158A (en) * | 2014-09-28 | 2015-03-04 | 中国科学院南海海洋研究所 | Method for breeding larvae-type oysters by oviparous-type means |
| CN105165686A (en) * | 2015-10-13 | 2015-12-23 | 中国科学院南海海洋研究所 | Method for cultivating double-black-shell crassostrea sikamea strain |
| CN110235827A (en) * | 2019-07-18 | 2019-09-17 | 渤海水产股份有限公司 | A kind of parent shrimp rearing method of high salinity mariculture prawn |
| CN110292011A (en) * | 2019-08-16 | 2019-10-01 | 渤海水产股份有限公司 | A kind of suitable salt-soda soil cultured prawn seed shrimp breeding method |
| CN112136736A (en) * | 2020-11-03 | 2020-12-29 | 烟台和丰水产科技有限公司 | Breeding method of stress-resistant crassostrea gigas new variety |
| CN112772407A (en) * | 2021-01-29 | 2021-05-11 | 中国科学院南海海洋研究所 | Method for cultivating high-salt-resistant hybrid oyster new strain in south China |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658146A (en) * | 2008-08-25 | 2010-03-03 | 李思发 | Salt tolerant breeding technology for tilapia |
| CN102499130A (en) * | 2011-10-18 | 2012-06-20 | 中国水产科学研究院黄海水产研究所 | Breeding method of low-salt-resistant line of blue crab |
-
2014
- 2014-03-26 CN CN201410117201.XA patent/CN103891647B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658146A (en) * | 2008-08-25 | 2010-03-03 | 李思发 | Salt tolerant breeding technology for tilapia |
| CN102499130A (en) * | 2011-10-18 | 2012-06-20 | 中国水产科学研究院黄海水产研究所 | Breeding method of low-salt-resistant line of blue crab |
Non-Patent Citations (4)
| Title |
|---|
| 何义朝 等: "墨西哥湾扇贝稚贝对盐度的耐受力", 《海洋学报（中文版）》, vol. 21, no. 4, 13 July 1999 (1999-07-13), pages 87 - 89 * |
| 林丽华 等: "盐度对香港巨牡蛎摄食和代谢的影响", 《广东农业科学》, no. 11, 10 June 2012 (2012-06-10), pages 10 - 14 * |
| 薛凌展 等: "近江牡蜗耐盐性幼虫的选育", 《中国海洋湖沼学会、中国动物学会贝类学分会第十二次学术讨论会》, 30 September 2005 (2005-09-30), pages 82 * |
| 霍忠明 等: "香港巨牡蛎与近江牡蛎杂交及回交子代早期生长发育比较", 《水产学报》, vol. 37, no. 8, 15 August 2013 (2013-08-15), pages 1155 - 1161 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381158A (en) * | 2014-09-28 | 2015-03-04 | 中国科学院南海海洋研究所 | Method for breeding larvae-type oysters by oviparous-type means |
| CN105165686A (en) * | 2015-10-13 | 2015-12-23 | 中国科学院南海海洋研究所 | Method for cultivating double-black-shell crassostrea sikamea strain |
| CN110235827A (en) * | 2019-07-18 | 2019-09-17 | 渤海水产股份有限公司 | A kind of parent shrimp rearing method of high salinity mariculture prawn |
| CN110292011A (en) * | 2019-08-16 | 2019-10-01 | 渤海水产股份有限公司 | A kind of suitable salt-soda soil cultured prawn seed shrimp breeding method |
| CN112136736A (en) * | 2020-11-03 | 2020-12-29 | 烟台和丰水产科技有限公司 | Breeding method of stress-resistant crassostrea gigas new variety |
| CN112772407A (en) * | 2021-01-29 | 2021-05-11 | 中国科学院南海海洋研究所 | Method for cultivating high-salt-resistant hybrid oyster new strain in south China |
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