CN111771784A - Method for producing blister pearl and regenerative cell bead by using pinctada martensii golden-lip pearl culturing shell - Google Patents
Method for producing blister pearl and regenerative cell bead by using pinctada martensii golden-lip pearl culturing shell Download PDFInfo
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- CN111771784A CN111771784A CN202010788017.3A CN202010788017A CN111771784A CN 111771784 A CN111771784 A CN 111771784A CN 202010788017 A CN202010788017 A CN 202010788017A CN 111771784 A CN111771784 A CN 111771784A
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- 241001212699 Pinctada martensii Species 0.000 title claims abstract description 86
- 238000012258 culturing Methods 0.000 title claims abstract description 47
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 30
- 239000011324 bead Substances 0.000 title claims abstract description 22
- 230000001172 regenerating effect Effects 0.000 title claims abstract description 21
- 239000011049 pearl Substances 0.000 claims abstract description 307
- 238000009395 breeding Methods 0.000 claims abstract description 76
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 11
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 7
- 230000037005 anaesthesia Effects 0.000 claims abstract description 7
- 238000002324 minimally invasive surgery Methods 0.000 claims abstract description 5
- 230000003248 secreting effect Effects 0.000 claims abstract description 4
- 235000015170 shellfish Nutrition 0.000 claims description 15
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 239000003292 glue Substances 0.000 claims description 12
- 239000000853 adhesive Substances 0.000 claims description 10
- 230000001070 adhesive effect Effects 0.000 claims description 10
- 210000003205 muscle Anatomy 0.000 claims description 9
- 241000490567 Pinctada Species 0.000 claims description 8
- 239000002344 surface layer Substances 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims description 3
- 239000010410 layer Substances 0.000 claims description 2
- 230000003716 rejuvenation Effects 0.000 claims 5
- 210000001835 viscera Anatomy 0.000 claims 1
- 230000009278 visceral effect Effects 0.000 abstract description 8
- 239000002390 adhesive tape Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 210000001015 abdomen Anatomy 0.000 description 5
- 241001490476 Pinctada maxima Species 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000006578 abscission Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000272194 Ciconiiformes Species 0.000 description 1
- 235000005956 Cosmos caudatus Nutrition 0.000 description 1
- 244000293323 Cosmos caudatus Species 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for producing a blister pearl and a regenerative cell bead by using a pinctada martensii golden-lip series pearl culture. When the pearl of the pinctada martensii pearl-lip system pearl culturing shell is harvested, selecting the pearl culturing shell which is large in size, correct in shell shape, full in visceral mass and good in growth in the same batch of pearl culturing shells, examining a nucleus-remaining shell through an X-ray machine, performing anesthesia treatment, sucking out free pearls meeting the quality requirement by using a minimally invasive surgery, preserving a pearl sac, secreting nacre by using the preserved pearl sac to produce regenerated cell beads, and sticking a shell-attached pearl model to produce shell-attached pearls; the method comprises the following steps of directly sticking a shell-attached pearl model to produce a shell-attached pearl after anesthesia treatment of the enucleated shell, returning the operated pearl-attached shell to the sea area for culturing the pearl, and culturing for 12-15 months to obtain the shell-attached pearl and the regenerative cell pearl/or the shell-attached pearl. The invention can improve the utilization rate of the pinctada martensii for breeding the pinctada martensii and improve the economic benefit of pearl culture.
Description
Technical Field
The invention relates to a method for producing blister pearls and regenerative cell pearls, belongs to the technical field of aquaculture, is suitable for recycling harvested pearls of pinctada martensii pearl-cultivating shells, and is particularly suitable for producing blister pearls and regenerative cell pearls.
Background
Pinctada martensii (Pinctada martensii) is also called Hepu nacre, pearls cultured by the Pinctada martensii in coastal areas of northern gulf of China are called 'Nanzhu', and the Nanzhu yield accounts for more than 95% of the seawater pearl yield of China. The south pearl culture is the pillar industry in the coastal region of the peninsula of Renzhou in Guangdong province, the pearl culture area in the whole city of the Zhanjiang in Guangdong province exceeds 5.38 mu of a year at the peak of production, 4.5 million people in 10 villages and towns engage in the pearl industry, the annual output of the pearls is about 20 tons, and the annual output accounts for more than 70 percent of the national output. The pearl processing factory has more than 100 pearl processing factories in the whole market, a huge industrial chain of pearl shell seedling cultivation, mother shell cultivation, nucleus planting and pearl cultivation, pearl processing and pearl product sale is formed, the total yield of the whole industrial chain reaches billions of yuan, and the method makes positive contribution to poverty removal, enrichment causing and export foreign exchange of coastal farmers in peninsula of Rezhou. The pearl produced by Pinctada martensii is called Chinese south pearl, the pearl produced by the remained pearl sac after the pearl is taken out is called regenerative cell bead, and is called Keshi abroad, so the Keshi produced by Pinctada martensii is called Chinese south Keshi bead.
At present, the main problems of the 'south pearl' production in China are that breeding varieties are degraded, breeding environments are deteriorated, breeding technologies are lack of innovation, the death rate of pearl breeding is high, the nucleus remaining rate is low, the pearl quality is poor, the selling price of original pearls is low, and the positivity of pearl breeding of pearl farmers is seriously influenced. The annual output of the Nanzhu is reduced from 30 tons to 2-3 tons at the peak of production, the culture benefit is greatly reduced, and farmers are switched to move or close over one another. In order to promote agricultural efficiency, increase income of farmers and rural stability and promote sustainable healthy development of the Nanzhu industry, pearl culture technology must be innovated, the industrial chain is prolonged, and a new economic growth point is formed.
Disclosure of Invention
Aiming at the condition that pearl of a pearl culturing shell is killed and not recycled after the pearl is harvested in the pearl production of the pinctada martensii, the invention aims to provide a method for recycling the pinctada martensii after the pearl is harvested, has remarkable economic, social and ecological benefits and can be used for the pearl production of the pinctada martensii.
The technical scheme adopted by the invention is as follows:
a method for producing a blister pearl and a regenerative cell bead by using a pinctada martensii golden-lip pearl culture shell comprises the following steps:
1) selecting the pearl-breeding shells: when the pinctada martensii golden lip series pearl-breeding shell pearls are harvested, selecting large and well-growing pearl-breeding shells in the same batch of pearl-breeding shells;
2) sucking out pearls: examining the pearl-breeding shell selected in the step 1) by X-ray, carrying out anesthesia treatment on the nucleus-remaining shell, and tying the nucleus-remaining shell after muscle is relaxed, sucking out free pearls of the Pinctada martensii dunker meeting the quality requirement by using a minimally invasive surgery, preserving a pearl sac, and producing regenerative cell beads by using the preserved pearl sac;
3) pearl model paste with shell (nucleated shell): sticking a shell-attached pearl model to the most concave part of the inner surface of the shell of the pearl-breeding shell taken out from the step 2) to produce a shell-attached pearl;
4) pearl model sticking with shell (core-removed shell): sticking a shell-attached pearl model on the most concave surface in the shell to produce a shell-attached pearl after the anesthetic plug port of the denucleated shell without the core obtained by X-ray inspection in the step 2);
5) and (3) breeding management of the pearl shells: moving the pearl oyster cultured after the operation of the step 3) and the step 4) to a sea area, and managing according to a conventional pearl oyster culture method, wherein the culture time is 12-15 months.
Furthermore, in the method for producing the shelled pearls and the regenerated cell pearls by using the golden-lip pearl-culturing shell of the pinctada martensii, in the step 1), the large-sized and well-grown pinctada martensii is a large-sized pinctada martensii in the same batch of pinctada martensii, and the pinctada martensii is full in visceral mass, regular and complete in shell shape, free of perforation on the shell, free of shedding on the surface layer, smooth in shell surface and obvious in growth line.
Further, in the method for producing the shelled pearls and the regenerated cell beads by using the pinctada martensii golden-lip-system pearl cultures, in the step 2), the X-ray inspection is performed by using a conveyor belt type X-ray machine.
Further, the method for producing the shelled pearls and the regenerated cell pearls by using the gold-lip pearl-culturing shell of the pinctada martensii comprises the steps of 1), arranging the pinctada martensii selected in the step 1) on a conveying belt of an X-ray machine, and sorting the pinctada martensii containing one pearl, two pearls and no pearl into a first holding device, a second holding device basket and a third holding device by an inspector when the pinctada martensii pass through the X-ray machine;
anaesthetizing pearl-culturing shells with pearls left in the first containing device and the second containing device, plugging the opening after the muscle is relaxed, sucking the pearls in the pearl-culturing shells in the first containing device by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping anti-inflammatory liquid medicine to the wound for disinfection;
the second operator sucks out the pearls in the pearl-breeding shells in the second containing device by using a special vacuum pearl sucker, finishes the wound by using a flat needle to be closed, and then drips the anti-inflammatory liquid medicine on the wound for disinfection treatment.
In the method for producing the shelled pearls and the regenerated cell pearls by using the pinctada martensii gold-lip-series pearl cultures, step 3) and step 4) are performed, the shelled pearl models are models in various shapes required by the market, the third operator takes out the pearl-culturing shell pasting model after the pearls are taken out by the first operator, the fourth operator takes out the pearl-culturing shell pasting model after the pearls are taken out by the second operator, the fifth operator pastes the enucleated shellfish pasting model contained in the third containing device by using special glue or double-sided glue.
Furthermore, the method for producing the pearl with the shell and the regenerated cell bead by using the pinctada martensii golden lip system pearl culture is also called pinctada martensii golden lip system, and is the golden-yellow mother pearl layer in the pinctada martensii shell.
Compared with the prior art, the invention has the following beneficial effects:
1) at present, 1.5-2-year Pinctada martensii dunker is selected for seawater pearl production in China to carry out nucleus implanting operation, one or two pearl nuclei are implanted into each shellfish according to the shellfish specification, the operated pearl-breeding shellfish is cultured in an sea area for 10-12 months, then the shellfish is killed to obtain the pearl, and the pearl-breeding shellfish is not recycled.
2) When the Pinctada martensii pearls are harvested, Pinctada martensii pearls which are healthy in shell body and good in growth are selected to cultivate pearl shells for the second time, pearl which meets the quality requirement is sucked out of nucleus-retaining shells through a minimally invasive surgery, pearl sacs are preserved, and formed pearl sacs are used for continuously secreting nacres to produce regenerated cell pearls, namely Keshi.
Because the original pearl sac is utilized, cell chips do not need to be implanted, the time from the nucleus implantation to the pearl sac formation is shortened, the pearl production time is short, the produced high-quality cell beads (Keshi) can be used for producing high-grade artware, and the high-grade pearl powder with irregular shape can be used for producing pearl health care products and cosmetics; meanwhile, sticking a model on the inner surface of the shell to produce a shell-attached pearl; the shell-attached pearl is produced by directly sticking the core-removed shell to a model.
3) The invention utilizes the pinctada martensii to produce the blister pearls, changes the current situation that the seawater blister pearls are used for producing large blister pearls mainly cultivated for pinctada maxima, pinctada maxima and penguin pinctada maxima, enriches the varieties of pearl products and provides more choices for pursuing individuation and diversification of pearl ornaments for people.
4) The invention utilizes the Pinctada martensii pearl culturing shell which is not recycled after the pearl is harvested to produce the blister pearl and the regenerative cell pearl, thereby improving the utilization rate of the Pinctada martensii pearl culturing shell, improving the economic benefit of Pinctada martensii pearl culturing and reducing the pressure of the cultured Pinctada martensii on the environment; the method is convenient to operate, easy to popularize, suitable for pearl culture production of pinctada martensii, and remarkable in economic, social and ecological benefits.
Detailed Description
The present invention is further illustrated in detail by the following examples, which are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.
The invention discloses a method for producing a blister pearl and a regenerative cell bead by using a pinctada martensii golden-lip series pearl culture, which comprises the following basic steps:
selecting pinctada martensii gold-lip-series pearl cultivators, sucking out free pearls, adhering shell-attached pearl models, and managing postoperative pearl cultivators; when the pinctada martensii pearl is harvested, selecting the pinctada martensii pearl-cultivating pearl with large individual, correct shell shape, full visceral mass and good growth from among the pinctada martensii pearl-cultivating pearls in the same batch, detecting the nucleus-remaining pearl through an X-ray machine, performing anesthesia treatment, sucking out free pearls meeting the quality requirement by using a minimally invasive surgery, preserving a pearl sac, secreting nacre by using the preserved pearl sac to produce regenerated cell beads, and sticking a shell-attached pearl model to produce shell-attached pearls.
The method comprises the following steps of directly sticking a shell-attached pearl model to produce a shell-attached pearl after anesthesia treatment of the enucleated shell, returning the operated pearl-attached shell to the sea area for culturing the pearl, and culturing for 12-15 months to obtain the shell-attached pearl and the regenerative cell pearl/or the shell-attached pearl. The invention can improve the utilization rate of the pinctada martensii for breeding the pinctada martensii and improve the economic benefit of pearl culture.
The method for producing the blister pearl and the regenerative cell bead by using the pinctada martensii golden-lip pearl culture comprises the following basic steps:
1) selecting the pearl-breeding shells: when the pinctada martensii gold-lip system pearl-breeding shell pearls are harvested, the pinctada martensii which is large in size, full in visceral mass, regular and complete in shell shape, free of perforation of shells, free of shedding of surface layers, smooth in shell surfaces and obvious in growth line in the same batch of the pinctada martensii is selected; the selected pearl culturing shells are arranged on a conveying belt of an X-ray machine, and an inspector sorts the pearl culturing shells containing one pearl, two pearls and no pearl to a first containing device, a second containing device and a third containing device when the pearl culturing shells pass through the X-ray machine;
2) sucking out pearls: anaesthetizing the pearl-breeding shells with pearls left in the first containing device and the second containing device in the step 1) to be tied after the muscles are relaxed, sucking the pearls in the pearl-breeding shells in the first containing device out by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping anti-inflammatory liquid medicine into the wound to perform disinfection treatment; sucking out pearls in pearl-breeding shells in a second containing device by a second operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping anti-inflammatory liquid medicine at the wound for disinfection treatment;
3) adhering shell pearl models: a third operator takes out the pearl-breeding shell pasting model after pearl from the first operator in the step 2), a fourth operator takes out the pearl-breeding shell pasting model after pearl from the second operator in the step 2), a fifth operator pastes the nucleus-removed shell pasting model contained in the third containing device, the model pasting uses special glue or double-faced adhesive tape, one side of the glue or the double-faced adhesive tape is pasted on the surface of the model, the other side of the glue or the double-faced adhesive tape is pasted on the most concave part of the inner surface of the shell, 1 model is pasted on the left shell and 1 model is pasted on the right shell of each pearl-breeding shell, and the models are arranged in a staggered mode;
4) and (3) breeding management of the pearl shells: and (3) putting the pearl shells after the operation of the step 3) into a cage, fixing the bellies upwards, moving the pearl shells to the sea area, and managing according to a conventional pearl shell culture method, wherein the pearl culture time is 12-15 months.
The method for producing the Pinctada martensii dunker and the regenerative cell bead/or the nacre dunker can improve the utilization rate of Pinctada martensii dunker and improve the economic benefit of pearl cultivation. The method is suitable for producing the Pinctada martensii pearls, is simple and convenient to operate, is easy to popularize in production enterprises and farmers, and has remarkable economic, social and ecological benefits.
Example 1
A method for producing a blister pearl and a regenerative cell bead by using a pinctada martensii golden-lip pearl culture shell specifically comprises the following steps:
1) selecting the pearl-breeding shells: when the pinctada martensii gold-lip system pearl-breeding shell pearls are harvested, the pinctada martensii which is large in size, full in visceral mass, regular and complete in shell shape, free of perforation of shells, free of shedding of surface layers, smooth in shell surfaces and obvious in growth line in the same batch of the pinctada martensii is selected; the selected pearl culturing shells are arranged on a conveyor belt of an X-ray machine, and inspectors sort the pearl culturing shells containing one pearl, two pearls and no pearl into a basket 1, a basket 2 and a basket 3 when the pearl culturing shells pass through the X-ray machine.
2) Sucking out pearls: anesthetizing the pearl-bearing shellfish retaining pearls in the baskets 1 and 2 in the step 1) by a tap operator, plugging the pearl-bearing shellfish after the muscle is relaxed, sucking out the pearls in the pearl-bearing shellfish in the basket 1 by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping an anti-inflammatory liquid medicine into the wound for disinfection treatment; the second operator sucks out the pearls in the pearl culturing shells in the basket 2 by using a special vacuum pearl sucker, finishes the wound by using a flat needle to close the wound, and then drips the anti-inflammatory liquid medicine on the wound to perform disinfection treatment.
3) Adhering shell pearl models: the third operator takes out the pearl-breeding shell pasting model after pearl from the first operator in the step 2), the fourth operator takes out the pearl-breeding shell pasting model after pearl from the second operator in the step 2), the fifth operator pastes the core-removed shell pasting model in the basket 3 by using special glue, one side of the glue is stuck on the surface of the model, the other side of the glue is stuck on the most concave part of the inner surface of the shell, 1 model is stuck on the left shell and 1 model is stuck on the right shell of each pearl-breeding shell, and the models are arranged in a staggered mode.
4) And (3) breeding management of the pearl shells: and (3) putting the pearl oyster cultured in the step 3) into a cage, fixing the belly upwards, moving to the sea area, and managing according to a conventional pearl oyster culturing method, wherein the culturing time is 12 months.
Example 2
A method for producing blister pearls and regenerated cell pearls by using pinctada martensii gold-lip-series pearl cultures comprises the following basic steps:
1) selecting the pearl-breeding shells: when the pinctada martensii gold-lip system pearl-breeding shell pearls are harvested, the pinctada martensii which is large in size, full in visceral mass, regular and complete in shell shape, free of perforation of shells, free of shedding of surface layers, smooth in shell surfaces and obvious in growth line in the same batch of the pinctada martensii is selected; the selected pearl culturing shells are arranged on a conveyor belt of an X-ray machine, and inspectors sort the pearl culturing shells containing one pearl, two pearls and no pearl into a basket 1, a basket 2 and a basket 3 when the pearl culturing shells pass through the X-ray machine.
2) Sucking out pearls: anesthetizing the pearl-bearing shellfish which is left with pearls in the basket 1 and the basket 2 in the step 1) by a mouth gag, plugging the mouth after the muscle is relaxed, sucking out the pearls in the pearl-bearing shellfish in the basket 1 by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping an anti-inflammatory liquid medicine into the wound to perform disinfection treatment; the second operator sucks out the pearls in the pearl culturing shells in the basket 2 by using a special vacuum pearl sucker, finishes the wound by using a flat needle to close the wound, and then drips the anti-inflammatory liquid medicine on the wound to perform disinfection treatment.
3) Adhering shell pearl models: the third operator takes out the pearl-breeding shell pasting model after pearl from the first operator in the step 2), the fourth operator takes out the pearl-breeding shell pasting model after pearl from the second operator in the step 2), the fifth operator pastes the nucleus-removed shell pasting model in the basket 3 by using special double-sided adhesive tape, one side of the double-sided adhesive tape is pasted on the surface of the model, the other side of the double-sided adhesive tape is pasted on the most concave part of the inner surface of the shell, 1 model is pasted on the left shell and 1 model is pasted on the right shell of each pearl-breeding shell, and the models are arranged in a staggered mode.
4) And (3) breeding management of the pearl shells: and (3) putting the pearl oyster cultured in the step 3) into a cage, fixing the belly upwards, moving to the sea area, and managing according to a conventional pearl oyster culturing method, wherein the culturing time is 15 months.
The following are contents of the effect test examples.
Effect test example 1
When the golden lip pearl-breeding pearl of pinctada martensii is harvested in a Yangtze Ridgeon river sand farm, Ronghui pearl company selects 1 ten thousand pearl-breeding pearls with large individuals, full visceral masses, right shell shapes, no perforated shells, no shedding surface layers, smooth shell surfaces and obvious growth lines, then the selected pearl-breeding pearls are arranged on a conveying belt of an X-ray machine, an inspector of the pearl-breeding pearls passes through the X-ray machine to sort the pearl-breeding pearls containing one pearl into a basket 1, 6072 pearl-breeding pearls containing two pearls into a basket 2 and 820 pearl-breeding pearls without pearl into a basket 3.
Anesthetizing pearl-breeding shells with pearls left in the basket 1 and the basket 2 by a tap operator, plugging the opening after the muscles are relaxed, sucking the pearls in the pearl-breeding shells in the basket 1 by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping an anti-inflammatory liquid medicine to the wound for disinfection treatment; the second operator sucks out the pearls in the pearl culturing shells in the basket 2 by using a special vacuum pearl sucker, finishes the wound by using a flat needle to close the wound, and then drips the anti-inflammatory liquid medicine on the wound to perform disinfection treatment.
The third operator takes 3108 pearl-culturing shell adhesive models after the pearls are taken out by the first operator, the fourth operator takes 6072 pearl-culturing shell adhesive models after the pearls are taken out by the second operator, the fifth operator pastes 820 enucleated shell adhesive models in the basket 3, the model adhesive uses the special glue, one surface of the special glue is pasted on the surface of the model, the other surface of the special glue is pasted on the most concave part of the inner surface of the shell, 1 left shell adhesive model and 1 right shell adhesive model of each pearl-culturing shell are arranged in a staggered mode.
10000 of pearl-breeding shellfish after the operation are only arranged in a piece cage, the belly of the pearl-breeding shellfish is upwards fixed, the pearl-breeding shellfish is moved to the sea area to be managed according to the conventional pearl-breeding method, the pearl is harvested after 12 months of breeding time, and the pearl-breeding result is shown in the following table 1.
TABLE 1 Pinctada martensii pearl cultivation results
| Bead-raising method | Time/month for growing pearl | Free beads/kg | Pearl with shell/ten thousand | Regenerative cell bead/kg |
| Conventional pearl culture | 12 | 3.35 | 0 | 0 |
| Effect experiment example 1 | 12 | 3.35 | 1.82 (abscission 0.18) | 2.34 |
Note: the number of model drops includes the number of models of dead pearl-breeding shells and the number of models of natural drops.
Effect test example 2
When the golden lip pearl of pinctada martensii is harvested in Xuwen county, western town of Zhanjiang, Ronghui pearl company, 12000 pinctada martensii pearl-breeding pearls with large individual, full visceral mass, upright shell shape, no perforation on shell, no drop on surface layer, smooth shell surface and obvious growth line are selected, the pinctada martensii is arranged on a conveying belt of an X-ray machine, an inspector passes through the X-ray machine to sort the pinctada martensii containing one pearl into 3806 pearls in total in a basket 1, sort the pinctada martensii containing two pearls into 7260 pearls in total in a basket 2, and sort the enucleated pinctada martensii without pearl into 934 pearls in total in a basket 3.
Anesthetizing pearl-breeding shells with pearls left in the basket 1 and the basket 2 by a tap operator, plugging the pearl-breeding shells after the muscles are relaxed, sucking the pearls in the pearl-breeding shells in the basket 1 out by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping an anti-inflammatory liquid medicine into the wound to perform disinfection treatment; the second operator sucks out the pearls in the pearl culturing shells in the basket 2 by using a special vacuum pearl sucker, finishes the wound by using a flat needle to close the wound, and then drips the anti-inflammatory liquid medicine on the wound to perform disinfection treatment.
The third operator takes out 3806 pearl-breeding shell pasting models after the pearl is taken out by the first operator, the fourth operator takes out 7260 pearl-breeding shell pasting models after the pearl is taken out by the second operator, the fifth operator pastes the decored shell 934 models, the models are pasted by using special double-sided adhesive, one side of the double-sided adhesive is pasted on the surface of the models, the other side of the double-sided adhesive is pasted on the most concave part of the inner surface of the shell, 1 model is pasted on the left shell and 1 model is pasted on the right shell of each pearl-breeding shell, and the models are arranged in a staggered mode.
12000 of the pearl-breeding shell after the operation is only arranged in a piece cage, the belly of the pearl-breeding shell is upwards fixed, the pearl-breeding shell is moved to the sea area to be managed according to the conventional pearl-breeding method, the breeding time is 15 months, and the pearl breeding effect of the Pinctada martensii shell is shown in the following table 2.
TABLE 2 margarita pearl culture Effect
| Bead-raising method | Time/month for growing pearl | Free beads/kg | Pearl with shell/ten thousand | Regenerative cell bead/kg |
| Conventional pearl culture | 15 | 4.14 | 0 | 0 |
| Experimental examples of Effect | 15 | 4.14 | 2.15 (abscission 0.25) | 2.84 |
Note: the number of model drops includes the number of models of dead pearl-breeding shells and the number of models of natural drops.
As can be seen from the results of experimental examples 1 and 2, the pinctada martensii golden-lip-series pearl cultures of the blister pearls and the regenerative cell pearls, and the pearl cultures can harvest the blister pearls and the regenerative cell pearls besides normally harvesting free pearls. Therefore, the invention can improve the utilization rate of the pinctada martensii for breeding the pinctada martensii and improve the economic benefit of pearl culture.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (6)
1. A method for producing a blister pearl and a regenerative cell bead by using a pinctada martensii golden-lip pearl culture shell is characterized by comprising the following steps of: the method comprises the following steps:
1) selecting the pearl-breeding shells: when the pinctada martensii golden lip series pearl-breeding shell pearls are harvested, selecting large and well-growing pearl-breeding shells in the same batch of pearl-breeding shells;
2) sucking out pearls: examining the pearl-breeding shell selected in the step 1) by X-ray, carrying out anesthesia treatment on the nucleus-remaining shell, and tying the nucleus-remaining shell after muscle is relaxed, sucking out free pearls of the Pinctada martensii dunker meeting the quality requirement by using a minimally invasive surgery, storing a pearl sac, and secreting nacres by using the stored pearl sac to produce regenerative cell beads;
3) adhering shell pearl model with nucleus shell: sticking a shell-attached pearl model to the most concave part of the inner surface of the shell of the pearl-breeding shell taken out from the step 2) to produce a shell-attached pearl;
4) pasting the shell-attached pearl model with the shell removed from the shell: performing anesthesia treatment on the denucleated shell obtained by X-ray inspection in the step 2) without a core, and sticking a shell-attached pearl model on the most concave part of the inner surface of the shell to produce a shell-attached pearl;
5) and (3) breeding management of the pearl shells: moving the pearl oyster cultured after the operation of the step 3) and the step 4) to a sea area, and managing according to a conventional pearl oyster culture method, wherein the culture time is 12-15 months.
2. The method for producing a pearl with a shell and a rejuvenated cell using a pinctada martensii gold-labial pearl culture according to claim 1, wherein:
in the step 1), the large and well-grown pearl-culturing shell is a large pearl-culturing shell with a full internal organ ball, a regular and complete shell shape, no perforation on the shell, no shedding on the surface layer, a smooth shell surface and an obvious growth line in the same batch of pearl-culturing shells.
3. The method for producing a pearl with a shell and a rejuvenated cell using a pinctada martensii gold-labial pearl culture according to claim 1, wherein:
in the step 2), the X-ray inspection is performed by using a conveyor belt type X-ray machine.
4. The method for producing a pearl with a shell and a rejuvenated cell using a pinctada martensii gold-labial pearl culture according to claim 1, wherein:
arranging the pearl culturing shell selected in the step 1) on a conveying belt of an X-ray machine, and sorting the pearl culturing shell containing one pearl, two pearls and no pearl into a first containing device, a second containing device and a third containing device by an inspector when the pearl culturing shell passes through the X-ray machine;
anaesthetizing pearl-cultivating shells with pearls left in the first containing device and the second containing device, plugging the opening after the muscle is relaxed, sucking the pearls in the pearl-cultivating shells in the first containing device by a first operator by using a special vacuum pearl sucker, finishing the wound by using a flat needle to close the wound, and then dripping anti-inflammatory liquid medicine to the wound for disinfection treatment;
the second operator sucks out the pearls in the pearl-breeding shells in the second containing device by using a special vacuum pearl sucker, finishes the wound by using a flat needle to be closed, and then drips the anti-inflammatory liquid medicine on the wound for disinfection treatment.
5. The method for producing a pearl with a shell and a rejuvenated cell using a pinctada martensii gold-labial pearl culture according to claim 1, wherein:
in the steps 3) and 4), the shell-attached pearl model is a model with various shapes required by the market, a third operator takes out a pearl-culturing shell pasting model after the pearl is taken out by the first operator, a fourth operator takes out the pearl-culturing shell pasting model after the pearl is taken out by the second operator, a fifth operator pastes the nucleus-removed shell model contained in the third containing device, and the model is pasted by using special glue or double-sided adhesive.
6. The method for producing a pearl with a shell and a rejuvenated cell using a pinctada martensii gold-labial pearl culture according to claim 1, wherein:
the Pinctada martensii golden lip line is also called Pinctada martensii golden lip shellfish and refers to Pinctada martensii shells with golden pearl layers.
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| CN112931329A (en) * | 2021-02-23 | 2021-06-11 | 广东海洋大学 | Method for cultivating golden yellow free nucleated pearls |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426273Y (en) * | 2000-06-05 | 2001-04-11 | 李文钜 | Pearl nucleus detecting device for pearl shell |
| CN1513304A (en) * | 2003-08-18 | 2004-07-21 | 谢绍河 | Method for culturing pearls with speical shape and without kernels |
| CN102106267A (en) * | 2010-12-02 | 2011-06-29 | 广东海洋大学 | Cultivating method of hieroglyphic blister freshwater pearl |
| CN102511431A (en) * | 2012-01-10 | 2012-06-27 | 上海海洋大学 | Method for inducing hyriopsis cumingii to produce blister pearls and round pearls |
| CN104686415A (en) * | 2015-02-12 | 2015-06-10 | 南通奥斯特鞋业有限公司 | Full-nacrum nucleated pearl and quickly cultivation method thereof |
| CN104957066A (en) * | 2015-07-09 | 2015-10-07 | 海南大学 | One-body-three-use method for cultivating three types of pearls by means of pteria penguin |
| CN107014838A (en) * | 2017-04-01 | 2017-08-04 | 浙江科技学院 | The detector of pearl state in precious freshwater mussel is checked based on X-ray |
| CN110031486A (en) * | 2019-04-18 | 2019-07-19 | 广东海洋大学 | A kind of method of quick identification seawater and fresh water pearl powder |
| US20200015459A1 (en) * | 2018-07-10 | 2020-01-16 | Mercenaria LLC | Cultured bivalve mollusk pearls and methods of preparation therefor |
| CN111264429A (en) * | 2020-03-24 | 2020-06-12 | 谢郁 | Method for breeding 3D baroque pearls |
-
2020
- 2020-08-07 CN CN202010788017.3A patent/CN111771784A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426273Y (en) * | 2000-06-05 | 2001-04-11 | 李文钜 | Pearl nucleus detecting device for pearl shell |
| CN1513304A (en) * | 2003-08-18 | 2004-07-21 | 谢绍河 | Method for culturing pearls with speical shape and without kernels |
| CN102106267A (en) * | 2010-12-02 | 2011-06-29 | 广东海洋大学 | Cultivating method of hieroglyphic blister freshwater pearl |
| CN102511431A (en) * | 2012-01-10 | 2012-06-27 | 上海海洋大学 | Method for inducing hyriopsis cumingii to produce blister pearls and round pearls |
| CN104686415A (en) * | 2015-02-12 | 2015-06-10 | 南通奥斯特鞋业有限公司 | Full-nacrum nucleated pearl and quickly cultivation method thereof |
| CN104957066A (en) * | 2015-07-09 | 2015-10-07 | 海南大学 | One-body-three-use method for cultivating three types of pearls by means of pteria penguin |
| CN107014838A (en) * | 2017-04-01 | 2017-08-04 | 浙江科技学院 | The detector of pearl state in precious freshwater mussel is checked based on X-ray |
| US20200015459A1 (en) * | 2018-07-10 | 2020-01-16 | Mercenaria LLC | Cultured bivalve mollusk pearls and methods of preparation therefor |
| CN110031486A (en) * | 2019-04-18 | 2019-07-19 | 广东海洋大学 | A kind of method of quick identification seawater and fresh water pearl powder |
| CN111264429A (en) * | 2020-03-24 | 2020-06-12 | 谢郁 | Method for breeding 3D baroque pearls |
Non-Patent Citations (3)
| Title |
|---|
| 中国大百科全书总编辑委员会《农业》编辑委员会 等: "《中国大百科全书 农业 1-2》", 30 September 1990, 中国大百科全书出版社 * |
| 包章泰: "《珠宝鉴赏投资指南》", 30 September 2014, 中国书店 * |
| 崔文元 等: "《珠宝玉石学GAC教程》", 31 May 2006, 地质出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931329A (en) * | 2021-02-23 | 2021-06-11 | 广东海洋大学 | Method for cultivating golden yellow free nucleated pearls |
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