CN109006170A - A kind of bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria - Google Patents
A kind of bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria Download PDFInfo
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- CN109006170A CN109006170A CN201810997482.0A CN201810997482A CN109006170A CN 109006170 A CN109006170 A CN 109006170A CN 201810997482 A CN201810997482 A CN 201810997482A CN 109006170 A CN109006170 A CN 109006170A
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- bacterium bag
- acanthopore
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention provides a kind of bacterium bag production methods of Dictyophora rubrovalvata fast growing bacteria, this method is inoculated with using multiple spot and corresponding adjusting and optimizing culture base-material, corresponding control fermentation temperature and humidity, this method multiple spot while bacterium germination, increase germination point, bacterium bag bacterium germination speed is fast, bacterium germination shifts to an earlier date 20-30 days, and pollution is greatly reduced with method inoculation of the invention, pollution rate can reduce by 20% or so, and present invention mycelia growth by way of acanthopore is more healthy and stronger, and mycelia vigor is good, be conducive to the guarantee of later fruiting, fruiting can increase by 10% yield.
Description
Technical field
The invention belongs to technical field of agricultural cultivation, and in particular to a kind of bacterium bag production side of Dictyophora rubrovalvata fast growing bacteria
Method.
Background technique
Dictyophora phalloidea also known as bamboo fungus, bamboo ginseng, celestial being's umbrella, grenadine mushroom etc. belong to Holobasidiomycetidae, Gasteromycete, ghost in classification
Mesh, Phallaceae, dictyophora phalloidea category.According to records, which has 11 kinds at present, wherein can be edible have Dictyophora rubrovalvata, Cultivation of Dictyophora,
Short-skirted veiled lady and Dictyophora echino-volvata Zane, hyoscine have Dictyophora mulicolor Bork.et Br..Currently, can large-scale planting, with higher economic value
Dictyophora phalloidea type mainly has 3 kinds, is Cultivation of Dictyophora (D.indusiata), Dictyophora echino-volvata Zane (D.echino-volvata) and red respectively
It holds in the palm dictyophora phalloidea (D.rubrovolvata)
Dictyophora phalloidea is that one kind is full of nutrition, fragrant and sweet taste is dense, crisp agreeable to the taste edible mushroom." being eaten altogether with meat, delicious anti-corrosion ", tool
" color, taste, perfume (or spice), shape " four is exhausted, and wherein Dictyophora rubrovalvata is even more breath faint scent, and stem is plump, the thick eye of indusium arteries and veins is big tender and crisp and well-known,
Has effects that the help of similar ginseng simultaneously.
As research discovery dictyophora phalloidea of the people to dictyophora phalloidea is a kind of saprophytic bacteria, symbiosis is not present with the root system of bamboo class,
Saprogenesis is sought just with the deadwood and rotten leaf of bamboo plant, theoretic breakthrough has pushed the development of bamboo shoots industry, at present bamboo
The planting technology of sweet-smelling grass mainly switchs to substituting stuff cultivation, is obtained with agricultural and sideline products such as sawdust, cotton seed hulls, corncobs to plant dictyophora phalloidea
Development.But due to the biological characteristics mycelia slow growth of dictyophora phalloidea, Dictyophora rubrovalvata is relatively slow in numerous dictyophora phalloidea kinds again
Kind, how to allow Dictyophora rubrovalvata fast growing bacteria to guarantee its bacterium bag quality of production, institute again so we must study in production
It is exactly to be studied on this basis with invention, by multiple spot inoculation method, porous bacterium germination shortens the bacterium germination of bacterium bag
Time and cell age.
Summary of the invention
To overcome defect of the existing technology, the present invention provides a kind of bacterium bag production sides of Dictyophora rubrovalvata fast growing bacteria
Method, this method is inoculated with using multiple spot and corresponding adjusting and optimizing culture base-material, corresponding to control fermentation temperature and humidity, this method multiple spot
Bacterium germination simultaneously, increases germination point, and bacterium bag bacterium germination speed is fast, and bacterium germination shifts to an earlier date 20-30 days, and is inoculated with greatly with method of the invention
Reduce pollution greatly, pollution rate can reduce by 20% or so, and present invention mycelia by way of acanthopore grows more healthy and stronger, bacterium
Silk vigor is good, is conducive to the guarantee of later fruiting, fruiting can increase by 10% yield.
Specifically, the present invention provides a kind of bacterium bag production methods of Dictyophora rubrovalvata fast growing bacteria, comprising the following steps:
(1) prepared by compost: by weight percentage, formula are as follows: weed tree sawdust 60-80%, rice bran and person's wheat bran at least one
Plant 10-30%, corn flour 3-5%, bean cake powder and soybean powder at least one 2-3%, white sugar 0.5-1%, gypsum 0.5-1%, stone
Ash and precipitated calcium carbonate at least one 0.5-1%, ph value 6.0-7.0 calculate each object according to dress packet quantity according to above-mentioned formula
The weight of material, weighs up, and carries out mixing spice, and the spice time is 40-60min, moisture content 60-65%；
(2) it prepares solid bacterium bag: ingredient described in step (1) being prepared and starts to pack after stirring evenly, bag
Son uses resistance to high bag, is sealed after the completion of pack with edible fungus sealing machine and is tightened and tying cannot prick compost, finally existed
According to pricking an osculum with the needle of thick 0.5-1mm at cultivating bag tail end 3-5cm and be stained with non-woven rubberized fabric to ventilative；
(3) it sterilizes: step (2) being filled into the neatly packed pusher of sack-finished- bacterium bag and enters in autoclave to sterilize, sterilizing knot
The bacterium bag push-in cooling chamber of beam is cooled down, and being cooled to bacterium bag temperature can be inoculated at 25 DEG C.
(4) sterilized bacterium bag in step (3) is successively passed to degree of purification is the sterile of 1000 grades or more clean levels
In room, is wiped one time and is carried out disinfection in bacterium bag with antiseptic gauze, then successively beat 3-5 hole with the perforation rod of sanitized,
Punching distance is 6-10cm, and the diameter of perforation rod is 2-3cm, and perforation rod length is 10-15cm；
(5) bacterium bag having openning hole in step (4) is inoculated with, the strain bag disinfected is all removed, by strain point
Inoculation hole is filled at tapered strain；
(6) the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness again is 1-2 thick polyethylene plastic bags,
And tied with bundling machine, prevent strain from infecting.
(7) bacterium bag that the inoculation in step (6) finishes gently is transmitted and carries out dark culturing, temperature 22- into culturing room
26 DEG C, humidity 60-75%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 5-8cm when can carry out first time acanthopore, pierce
Hole syringe needle thickness is 2-3mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2-2.5cm, cannot prick mycelia
Otherwise the compost not grown to is easy microbiological contamination；
(9) a complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room, oxygen fills after bacterium bag acanthopore at this time
Foot, rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 20-24 DEG C, prevents fever from burning bacterium.After acanthopore
Rapidly, acanthopore method carries out an acanthopore again in same step (7) after cultivating in a fungus bag 20-30 days for mycelia growth, guarantees red support
Oxygen demand in dictyophora phalloidea growth and development process.
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into culturing room and notices that the temperature for adjusting culturing room is
20-23 DEG C, prevent mycelia fever from burning bacterium, band mycelia all covers with bacterium bag after-ripening and can be not only used for fruiting in 5-10 days.
Step (1) compost preparation described above: by weight percentage, formula are as follows: weed tree sawdust 70%, rice bran and person
Wheat bran at least one 20%, corn flour 4%, bean cake powder and soybean powder at least one 3%, white sugar 1%, gypsum %, lime and light
Matter calcium carbonate at least one 1%, ph value 6.0-7.0 calculate the weight of each material according to dress packet quantity according to above-mentioned formula, claim
It is good, mixing spice is carried out, the spice time is 50min, moisture content 63%；
Step (2) described above prepares solid bacterium bag: the high temperature resistant bag is refractory polyethylene or polypropylene
Bag.
It is 1000 grades or more that sterilized bacterium bag in step (2) is successively passed to degree of purification by step (4) described above
It in the desinfection chamber of clean level, is wiped one time and is carried out disinfection in bacterium bag with antiseptic gauze, then use the perforation rod of sanitized
4 holes are successively made a call to, punching distance is 8cm, and the diameter of perforation rod is 2.5cm, and perforation rod length is 12cm；
It is 1-2 thick that the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness by step (6) described above again
Polyethylene plastic bag, and tied with bundling machine, prevent strain from infecting.
The bacterium bag that inoculation in step (6) finishes gently is transmitted and carries out dark into culturing room by step (7) described above
Culture, temperature are 24 DEG C, humidity 68%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 7cm when can carry out first time acanthopore, acanthopore
Syringe needle thickness is 2-3mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2-2.5cm, cannot prick mycelia not
Otherwise the compost grown to is easy microbiological contamination；
A complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room by step (9) described above, at this time bacterium bag
Oxygen is sufficient after acanthopore, and rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 22 DEG C, prevents fever from burning
Bacterium, the mycelia growth after acanthopore rapidly, once pierced again in same step (7) after cultivating in a fungus bag 20-30 days by acanthopore method
Hole guarantees the oxygen demand in Dictyophora rubrovalvata growth and development process.
The bacterium bag that secondary acanthopore finishes in step (9) is put by step (10) described above to be paid attention to adjusting training in culturing room
The temperature for supporting room is 22 DEG C, prevents mycelia fever from burning bacterium, and band mycelia all covers with bacterium bag after-ripening and can be not only used for fruiting in 5-10 days.
The invention has the benefit that
The shortcomings that the present invention overcomes original vaccination ways to be a little inoculated with, and bacterium germination point is few, cell age difference, pervious one bag of bacterium
Packet cell age can be difference 2-3 months, with method multiple spot of the invention while bacterium germination, increase germination point, bacterium bag bacterium germination speed is fast, hair
Bacterium shifts to an earlier date 20-30 days, while cell age reduces 2-3 months, only differs 1-2 months, and is subtracted significantly with method inoculation of the invention
Pollution is lacked, pollution rate can reduce by 20% or so, and present invention mycelia growth by way of acanthopore is more healthy and stronger, and mycelia is living
Power is good, is conducive to the guarantee of later fruiting, and fruiting can increase by 10% yield.
Specific embodiment
To be further described further to beneficial effects of the present invention, the present invention is further elaborated in conjunction with the embodiments,
It should be understood that cited embodiment and test example of the invention is intended to illustrate advantageous effect of the invention, but the present invention
Protection scope be not restricted to this.
A kind of bacterium bag production method of the Dictyophora rubrovalvata fast growing bacteria of embodiment 1
(1) prepared by compost: by weight percentage, formula are as follows: weed tree sawdust 60%, rice bran and person's wheat bran are at least one
30%, corn flour 4%, bean cake powder and soybean powder at least one 3%, white sugar 1%, gypsum 1%, lime and precipitated calcium carbonate are at least
One kind 1%, ph value 6.0-7.0 calculates the weight of each material according to dress packet quantity according to above-mentioned formula, weighs up, mixed
Spice, spice time are 40min, moisture content 60%；
(2) it prepares solid bacterium bag: ingredient described in step (1) being prepared and starts to pack after stirring evenly, bag
Son uses resistance to high bag, is sealed after the completion of pack with edible fungus sealing machine and is tightened and tying cannot prick compost, finally existed
According to pricking an osculum with the needle of thick 0.5mm at cultivating bag tail end 3cm and be stained with non-woven rubberized fabric to ventilative；
(3) it sterilizes: step (2) being filled into the neatly packed pusher of sack-finished- bacterium bag and enters in autoclave to sterilize, sterilizing knot
The bacterium bag push-in cooling chamber of beam is cooled down, and being cooled to bacterium bag temperature can be inoculated at 25 DEG C；
(4) sterilized bacterium bag in step (3) is successively passed to degree of purification is the sterile of 1000 grades or more clean levels
It in room, is wiped one time and is carried out disinfection in bacterium bag with antiseptic gauze, then successively made a call to 3 holes with the perforation rod of sanitized, beat
Hole distance is 6cm, and the diameter of perforation rod is 2cm, and perforation rod length is 10cm；
(5) bacterium bag having openning hole in step (4) is inoculated with, the strain bag disinfected is all removed, by strain point
Inoculation hole is filled at tapered strain；
(6) the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness again is 1-2 thick polyethylene plastic bags,
And tied with bundling machine, prevent strain from infecting.
(7) bacterium bag that the inoculation in step (6) finishes gently is transmitted and carries out dark culturing, temperature 22 into culturing room
DEG C, humidity 60%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 5cm when can carry out first time acanthopore, acanthopore
Syringe needle thickness is 2mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2cm, cannot prick mycelia and not grow to
Compost, otherwise be easy microbiological contamination；
(9) a complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room, oxygen fills after bacterium bag acanthopore at this time
Foot, rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 20 DEG C, prevents fever from burning bacterium.Mycelia after acanthopore
Rapidly, acanthopore method carries out an acanthopore, guarantee Dictyophora rubrovalvata again in same step (7) after cultivating in a fungus bag 20-30 days for growth
Oxygen demand in growth and development process.
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into and notices that the temperature for adjusting culturing room is 20 in culturing room
DEG C, prevent mycelia fever from burning bacterium, band mycelia all covers with bacterium bag after-ripening and can be not only used for fruiting in 5-10 days, relative to conventional method,
Fruiting yield increases by 9.6%.
A kind of bacterium bag production method of the Dictyophora rubrovalvata fast growing bacteria of embodiment 2
(1) prepared by compost: by weight percentage, formula are as follows: weed tree sawdust 70%, rice bran and person's wheat bran are at least one
20%, corn flour 4%, bean cake powder and soybean powder at least one 3%, white sugar 1%, gypsum %, lime and precipitated calcium carbonate are at least
One kind 1%, ph value 6.0-7.0 calculates the weight of each material according to dress packet quantity according to above-mentioned formula, weighs up, mixed
Spice, spice time are 50min, moisture content 63%；
(2) it prepares solid bacterium bag: ingredient described in step (1) being prepared and starts to pack after stirring evenly, bag
Son uses resistance to high bag, is sealed after the completion of pack with edible fungus sealing machine and is tightened and tying cannot prick compost, finally existed
According to pricking an osculum with the needle of thick 0.8mm at cultivating bag tail end 4cm and be stained with non-woven rubberized fabric to ventilative；
(3) it sterilizes: step (2) being filled into the neatly packed pusher of sack-finished- bacterium bag and enters in autoclave to sterilize, sterilizing knot
The bacterium bag push-in cooling chamber of beam is cooled down, and being cooled to bacterium bag temperature can be inoculated at 25 DEG C.
(4) sterilized bacterium bag in step (3) is successively passed to degree of purification is the sterile of 1000 grades or more clean levels
It in room, is wiped one time and is carried out disinfection in bacterium bag with antiseptic gauze, then successively made a call to 4 holes with the perforation rod of sanitized, beat
Hole distance is 8cm, and the diameter of perforation rod is 2.5cm, and perforation rod length is 12cm；
(5) bacterium bag having openning hole in step (4) is inoculated with, the strain bag disinfected is all removed, by strain point
Inoculation hole is filled at tapered strain；
(6) the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness again is 1-2 thick polyethylene plastic bags,
And tied with bundling machine, prevent strain from infecting.
(7) bacterium bag that the inoculation in step (6) finishes gently is transmitted and carries out dark culturing, temperature 24 into culturing room
DEG C, humidity 68%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 7cm when can carry out first time acanthopore, acanthopore
Syringe needle thickness is 2.5mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2-2.5cm, cannot prick mycelia not
Otherwise the compost grown to is easy microbiological contamination；
(9) a complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room, oxygen fills after bacterium bag acanthopore at this time
Foot, rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 22 DEG C, prevents fever from burning bacterium.Mycelia after acanthopore
Rapidly, acanthopore method carries out an acanthopore, guarantee Dictyophora rubrovalvata again in same step (7) after cultivating in a fungus bag 20-30 days for growth
Oxygen demand in growth and development process.
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into and notices that the temperature for adjusting culturing room is 22 in culturing room
DEG C, prevent mycelia fever from burning bacterium, band mycelia all covers with bacterium bag after-ripening and can be not only used within 5-10 days fruiting, the more former routine of fruiting yield
Method improves 11.3%.
The bacterium bag production method of 3 Dictyophora rubrovalvata fast growing bacteria of embodiment
(1) prepared by compost: by weight percentage, formula are as follows: weed tree sawdust 80%, rice bran and person's wheat bran are at least one
10%, corn flour 5%, bean cake powder and soybean powder at least one 3%, white sugar 0.5%, gypsum 0.5%, lime and precipitated calcium carbonate
At least one 1%, ph value 6.0-7.0 calculates the weight of each material according to dress packet quantity according to above-mentioned formula, weighs up, carries out
Spice is mixed, the spice time is 60min, moisture content 65%；
(2) it prepares solid bacterium bag: ingredient described in step (1) being prepared and starts to pack after stirring evenly, bag
Son uses resistance to high bag, is sealed after the completion of pack with edible fungus sealing machine and is tightened and tying cannot prick compost, finally existed
According to pricking an osculum with the needle of thick 0.5-1mm at cultivating bag tail end 5cm and be stained with non-woven rubberized fabric to ventilative；
(3) it sterilizes: step (2) being filled into the neatly packed pusher of sack-finished- bacterium bag and enters in autoclave to sterilize, sterilizing knot
The bacterium bag push-in cooling chamber of beam is cooled down, and being cooled to bacterium bag temperature can be inoculated at 25 DEG C.
(4) sterilized bacterium bag in step (3) is successively passed to degree of purification is the sterile of 1000 grades or more clean levels
It in room, is wiped one time and is carried out disinfection in bacterium bag with antiseptic gauze, then successively made a call to 5 holes with the perforation rod of sanitized, beat
Hole distance is 10cm, and the diameter of perforation rod is 3cm, and perforation rod length is 15cm；
(5) bacterium bag having openning hole in step (4) is inoculated with, the strain bag disinfected is all removed, by strain point
Inoculation hole is filled at tapered strain；
(6) the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness again is 1-2 thick polyethylene plastic bags,
And tied with bundling machine, prevent strain from infecting.
(7) bacterium bag that the inoculation in step (6) finishes gently is transmitted and carries out dark culturing, temperature 26 into culturing room
DEG C, humidity 75%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 8cm when can carry out first time acanthopore, acanthopore
Syringe needle thickness is 3mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2.5cm, cannot prick mycelia and not grow
Otherwise compost extremely is easy microbiological contamination；
(9) a complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room, oxygen fills after bacterium bag acanthopore at this time
Foot, rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 24 DEG C, prevents fever from burning bacterium.Mycelia after acanthopore
Rapidly, acanthopore method carries out an acanthopore, guarantee Dictyophora rubrovalvata again in same step (7) after cultivating in a fungus bag 20-30 days for growth
Oxygen demand in growth and development process.
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into and notices that the temperature for adjusting culturing room is 23 in culturing room
DEG C, prevent mycelia fever from burning bacterium, band mycelia all covers with bacterium bag after-ripening and can be not only used within 5-10 days fruiting, the more former routine of fruiting yield
Method improves 10.2%.
Claims (9)
1. a kind of bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria, which comprises the following steps:
(1) prepared by compost: by weight percentage, formula are as follows: weed tree sawdust 60-80%, rice bran and person's wheat bran at least one 10-
30%, corn flour 3-5%, bean cake powder and soybean powder at least one 2-3%, white sugar 0.5-1%, gypsum 0.5-1%, lime and light
Matter calcium carbonate at least one 0.5-1%, ph value 6.0-7.0 calculates the weight of each material according to above-mentioned formula according to dress packet quantity
Amount, weighs up, and carries out mixing spice, and the spice time is 40-60min, moisture content 60-65%；
(2) it prepares solid bacterium bag: ingredient described in step (1) being prepared and starts to pack after stirring evenly, sack is adopted
It is sealed with resistance to high bag, after the completion of pack with edible fungus sealing machine and is tightened and tying cannot prick compost, finally according to cultivation
An osculum is pricked with the needle of thick 0.5-1mm at training bag tail end 3-5cm and is stained with non-woven rubberized fabric to ventilative；
(3) it sterilizes: step (2) being filled into the neatly packed pusher of sack-finished- bacterium bag and enters in autoclave to sterilize, sterilize end
Bacterium bag push-in cooling chamber is cooled down, and being cooled to bacterium bag temperature can be inoculated at 25 DEG C；
(4) sterilized bacterium bag in step (3) is successively passed in the desinfection chamber that degree of purification is 1000 grades or more clean levels,
Wiped one time and carried out disinfection in bacterium bag with antiseptic gauze, then successively beat 3-5 hole with the perforation rod of sanitized, punching away from
It is 2-3cm from the diameter for 6-10cm, perforation rod, perforation rod length is 10-15cm；
(5) bacterium bag having openning hole in step (4) is inoculated with, the strain bag disinfected is all removed, strain is divided into be in
The strain of taper fills in inoculation hole；
(6) the bacteria stick outside that inoculation finishes in step (5) is covered a layer thickness again is 1-2 thick polyethylene plastic bags, is used in combination
Bundling machine ties, and prevents strain from infecting；
(7) bacterium bag that the inoculation in step (6) finishes gently being transmitted and carries out dark culturing into culturing room, temperature is 22-26 DEG C,
Humidity is 60-75%, gas concentration lwevel < 3000ppm；
(8) by bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 5-8cm when can carry out first time acanthopore, pricker
Head thickness is 2-3mm, and acanthopore distance 1cm within the mycelia edge locates, and acanthopore depth is 2-2.5cm, cannot prick mycelia and not give birth to
Long compost extremely, is otherwise easy microbiological contamination；
(9) a complete bacterium bag of acanthopore in step (8) being put into hot stage culturing room, oxygen is sufficient after bacterium bag acanthopore at this time,
Rapidly, fever is obvious for mycelia growth, notices that the temperature of culturing room is controlled at 20-24 DEG C, prevents fever from burning bacterium.Mycelia after acanthopore
Rapidly, acanthopore method carries out an acanthopore, guarantee Dictyophora rubrovalvata again in same step (7) after cultivating in a fungus bag 20-30 days for growth
Oxygen demand in growth and development process；
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into and notices that the temperature for adjusting culturing room is 20-23 in culturing room
DEG C, prevent mycelia fever from burning bacterium, band mycelia all covers with bacterium bag after-ripening and can be not only used for fruiting in 5-10 days.
2. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (1) training
Nutriment preparation: by weight percentage, formula are as follows: weed tree sawdust 70%, rice bran and person's wheat bran at least one 20%, corn flour 4%,
Bean cake powder and soybean powder at least one 3%, white sugar 1%, gypsum %, lime and precipitated calcium carbonate at least one 1%, ph value 6.0-
7.0, the weight of each material is calculated according to dress packet quantity according to above-mentioned formula, weighs up, carries out mixing spice, the spice time is
50min, moisture content 63%.
3. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (2) system
Standby solid bacterium bag: the high temperature resistant bag is refractory polyethylene or Polypropylene Bag.
4. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (4) will
Sterilized bacterium bag is successively passed in the desinfection chamber that degree of purification is 1000 grades or more clean levels in step (2), with disinfection yarn
Cloth is wiped one time in bacterium bag and is carried out disinfection, and then successively makes a call to 4 holes with the perforation rod of sanitized, and punching distance is 8cm, is beaten
The diameter of hole stick is 2.5cm, and perforation rod length is 12cm.
5. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (6) will
Covering a layer thickness outside the bacteria stick that inoculation finishes in step (5) again is 1-2 thick polyethylene plastic bags, and is pricked with bundling machine
It is good, prevent strain from infecting.
6. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (7) will
The bacterium bag that inoculation in step (6) finishes, which is gently transmitted, carries out dark culturing into culturing room, and temperature is 24 DEG C, humidity 68%,
Gas concentration lwevel < 3000ppm.
7. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (8) is
By bacterium germination 20-30 days in step (7), when the diameter of mycelia it is long to 7cm when can carry out first time acanthopore, acanthopore syringe needle thickness is 2-
3mm, for acanthopore distance within the mycelia edge at 1cm, acanthopore depth is 2-2.5cm, cannot prick the culture that mycelia does not grow to
Otherwise material is easy microbiological contamination.
8. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: step (9) is
A complete bacterium bag of acanthopore in step (8) is put into hot stage culturing room, oxygen is sufficient after bacterium bag acanthopore at this time, mycelia growth
Rapidly, fever obviously, notices that the temperature of culturing room is controlled at 22 DEG C, prevents fever from burning bacterium, and the mycelia growth after acanthopore is rapid,
Acanthopore method carries out an acanthopore again in same step (7) after cultivating in a fungus bag 20-30 days, guarantees Dictyophora rubrovalvata growth and development process
In oxygen demand.
9. the bacterium bag production method of Dictyophora rubrovalvata fast growing bacteria according to claim 1, it is characterised in that: the step
(10) bacterium bag that secondary acanthopore finishes in step (9) is put into and notices that the temperature for adjusting culturing room is 22 DEG C in culturing room, prevented
Bacterium is burnt in mycelia fever, and band mycelia all covers with bacterium bag after-ripening and can be not only used for fruiting in 5-10 days.
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| CN111788992A (en) * | 2020-08-18 | 2020-10-20 | 湖南和平生物科技有限公司 | Production method of Dictyophora rubrovalvata |
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