CN109220542A - A kind of production method of Dictyophora rubrovalvata blanket type bacterium bed - Google Patents

A kind of production method of Dictyophora rubrovalvata blanket type bacterium bed Download PDF

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CN109220542A
CN109220542A CN201810996744.1A CN201810996744A CN109220542A CN 109220542 A CN109220542 A CN 109220542A CN 201810996744 A CN201810996744 A CN 201810996744A CN 109220542 A CN109220542 A CN 109220542A
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bacterium bed
blanket type
dictyophora rubrovalvata
type bacterium
strain
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CN109220542B (en
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庄继文
陈翠翠
巩玉辉
方晔
肖忠建
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Guizhou Golden Frog Dashan Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

The present invention provides a kind of production method of Dictyophora rubrovalvata blanket type bacterium bed, this method uses blanket type bacterium bed fermentation method, overcomes the mode of current Dictyophora rubrovalvata bacterium bag production, and significantly economical production material, entire production cost reduce 10%-20%;Sandwich-type vaccination ways through the invention, bacterium germination is fast, and cell age is uniform, and the 30-40 days bacterium germination time of Dictyophora rubrovalvata can send out bacterium bed full, can be used to fruiting use, is particularly suitable for promoting the use of.

Description

A kind of production method of Dictyophora rubrovalvata blanket type bacterium bed
Technical field
The invention belongs to technical field of agricultural cultivation, and in particular to a kind of production method of Dictyophora rubrovalvata blanket type bacterium bed.
Background technique
Dictyophora phalloidea also known as bamboo fungus, bamboo ginseng, celestial being's umbrella, grenadine mushroom etc. belong to Holobasidiomycetidae, Gasteromycete, ghost in classification Mesh, Phallaceae, dictyophora phalloidea category.According to records, which has 11 kinds at present, wherein can be edible have Dictyophora rubrovalvata, Cultivation of Dictyophora, Short-skirted veiled lady and Dictyophora echino-volvata Zane, hyoscine have Dictyophora mulicolor Bork.et Br..Currently, can large-scale planting, with higher economic value Dictyophora phalloidea type mainly has 3 kinds, is Cultivation of Dictyophora (D.indus iata), Dictyophora echino-volvata Zane (D.echino-volvata) and red respectively It holds in the palm dictyophora phalloidea (D.rubrovolvata)
Dictyophora phalloidea is that one kind is full of nutrition, fragrant and sweet taste is dense, crisp agreeable to the taste edible mushroom." being eaten altogether with meat, delicious anti-corrosion ", tool " color, taste, perfume (or spice), shape " four is exhausted, and wherein Dictyophora rubrovalvata is even more breath faint scent, and stem is plump, the thick eye of indusium arteries and veins is big tender and crisp and well-known, Has effects that the help of similar ginseng simultaneously.
As research discovery dictyophora phalloidea of the people to dictyophora phalloidea is a kind of saprophytic bacteria, symbiosis is not present with the root system of bamboo class, Saprogenesis is sought just with the deadwood and rotten leaf of bamboo plant, theoretic breakthrough has pushed the development of bamboo shoots industry, at present bamboo The planting technology of sweet-smelling grass mainly switchs to substituting stuff cultivation, is obtained with agricultural and sideline products such as sawdust, cotton seed hulls, corncobs to plant dictyophora phalloidea Development.For how easy production dictyophora phalloidea strain is particularly important, the present invention is directed to the strain production method of dictyophora phalloidea, utilizes The biological characteristics of edible mushroom and dictyophora phalloidea formulate a kind of blanket type bacterium germination method, so that the production of Dictyophora rubrovalvata strain and later period Plantation can be more effective and rapid.
Summary of the invention
Specifically, the present invention provides a kind of production methods of Dictyophora rubrovalvata blanket type bacterium bed, comprising the following steps:
(1) blanket type bacterium bed raw material disposal, by weight percentage, by 50-60% weed tree sawdust, 10-20% cotton seed hulls, 10-30% rice bran and wheat bran are at least one, 3-5% corn flour, and 2-3% bean cake powder and soybean powder are at least one, and 0.5-1% is white Sugar, 0.5-1% gypsum, 0.5-1% lime calculate the weight of each material according to dress packet quantity according to above-mentioned formula, weigh up, into Row mixes spice, and adjusting pH value is 6.0-7.0, starts to carry out after stirring evenly to add water, makes water content 70-76%, adjusts PH is 9-10;
(2) it builds heap fermentation: uniformly mixed raw material being piled into wide 1.0-1.5m, the material heap of high 1.0-1.2m is used after building heap Diameter is that the wooden stick of 5-8cm pricks upper vent hole in material heap, and every 30-35cm inserts a hole, be between Kong Yukong it is Chinese character pin-shaped, in favor of Aerobic fementation carries out overlay film moisturizing, and turning is carried out when material heap reaches 60-70 DEG C, and whole fermentation process is 7-10 days, the high warm season Section turning turning in 1-2 days 1 time, low temperature season turning in 2-3 days 1 time, whole turning 3-5 times after fermentation unplugs material heap, Cooling, exhaust gas to be discharged are shakeout, the production of blanket type bacterium bed can be carried out when material temperature is down to 20 DEG C or so.
(3) production of blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is by strain With bacterium bed matrix made of compost, third layer is PE leaching membrane paper;The size of bacterium bed is but not limited to this wide 90-120cm, length 2-3m;
(4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 90-120cm in carrier chain upper berth, in non-woven fabrics The Dictyophora rubrovalvata strain of upper berth 1-1.5cm thickness, and strain is flattened with stainless steel platen machine, then repave 2- in the bacterium bed of strain The fermentation material of 3cm thickness, and flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1-1.5cm thickness that tiles again on the compost of pressing is used in combination Trigger squeeze compacting, flattens;
(5) one layer of PE leaching membrane paper will be covered above the bacterium bed flattened in step (4);, and the bacterium that will be made with beamer Bed, which all rolls, is put into culturing room's progress bacterium germination;
(6) culture room temperature is set as 22-26 DEG C, humidity 60-70%, and gas concentration lwevel < 5000ppm is cultivated herein Lower culture can form Dictyophora rubrovalvata blanket type bacterium bed for 30-40 days, can be used to soil fruiting after bacterium germination.
More specifically, described step (1) the blanket type bacterium bed raw material disposal, by weight percentage, by 55% weedtree Bits, 15% cotton seed hulls, 20% rice bran and wheat bran are at least one, 5% corn flour, and 2.5% bean cake powder and soybean powder are at least one, 1% white sugar, 1% gypsum, 0.5% lime calculate the weight of each material according to dress packet quantity according to above-mentioned formula, weigh up, into It goes and mixes spice, adjusting pH value is 65, starts to carry out after stirring evenly to add water, makes water content 73%, and adjusting PH is 9.5;
The step (2) builds heap fermentation: uniformly mixed raw material is piled wide 1.0-1.5m, the material of high 1.0-1.2m Heap is built after heap and pricks upper vent hole in material heap with the wooden stick that diameter is 5-8cm, and it is in product between Kong Yukong that every 30-35cm, which inserts a hole, Font carries out overlay film moisturizing in favor of aerobic fementation, and turning is carried out when material heap reaches 65 DEG C, and whole fermentation process is 8 days, After fermentation, material heap is pulled out for high temperature season turning turning in 1-2 days 1 time, low temperature season turning in 2-3 days 1 time, whole turning 4 times It opens, shakeouts cooling, exhaust gas to be discharged, the production of blanket type bacterium bed can be carried out when material temperature is down to 20 DEG C or so.
The production of described (3) the blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is The bacterium bed matrix made of strain and compost, third layer are ventilative PE film;The size of bacterium bed is width 100cm, length 2.5m.
The step (4) is according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 100cm in carrier chain upper berth, The Dictyophora rubrovalvata strain of non-woven fabrics upper berth 1.2cm thickness, and strain is flattened with stainless steel platen machine, then in the bacterium bed of strain again The fermentation material of 2.5cm thickness is spread, and is flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1.2cm thickness that tiles again on the compost of pressing is simultaneously It is compacted, is flattened with trigger squeeze;The PE leaching membrane paper for finally repaving a slice width 100cm plays moisturizing, ventilative effect;And use spool Machine is spooled, and is put into culturing room;
Described step (6) the culture room temperature is set as 23-25 DEG C, humidity 63-67%, and gas concentration lwevel < 4000ppm, Dictyophora rubrovalvata blanket type bacterium bed can be formed for 30-40 days by cultivating under this culture, can be used to cover after bacterium germination Native fruiting.
Described step (6) the culture room temperature is set as 24 DEG C, humidity 65%, gas concentration lwevel < 3000ppm, herein Culture lower culture can form Dictyophora rubrovalvata blanket type bacterium bed in 30-40 days, can be used to soil fruiting after bacterium germination.
The invention has the benefit that
The present invention overcomes the mode of current Dictyophora rubrovalvata bacterium bag production, significantly economical production material, and uses Pasteur The mode of biofermentation is sterilized to sterilize, traditional normal pressure or high pressure sterilization mode is changed, saves bio-fuel And the labour cost of sterilizing, entire production cost reduce 10%-20%;Sandwich-type vaccination ways through the invention, bacterium germination is fast, bacterium Age is uniform, while reducing the bacterium germination time of strain, needs 3-5 months using bacterium bag cultivation mode bacterium germination, and red with the present invention The 30-40 days bacterium germination time of support dictyophora phalloidea can send out bacterium bed full, can be used to fruiting and use.And the material that the present invention uses is all It is degradable biological environment-friendly materials, when fruiting entirely spreads bacterium bed out, soil fruiting, time saving and energy saving less manpower.And The cell age of strain is consistent, so the time of fruiting than more consistent, is conducive to management of producing mushroom and the harvesting in later period.
Detailed description of the invention
1 Dictyophora rubrovalvata blanket type bacterium bed schematic diagram of attached drawing
Specific embodiment
To be further described further to beneficial effects of the present invention, the present invention is further elaborated in conjunction with the embodiments, It should be understood that cited embodiment and test example of the invention is intended to illustrate advantageous effect of the invention, but the present invention Protection scope be not restricted to this.
A kind of production method of the Dictyophora rubrovalvata blanket type bacterium bed of embodiment 1
(1) blanket type bacterium bed raw material disposal, by weight percentage, by 50% weed tree sawdust, 10% cotton seed hulls, 30% meter Chaff and wheat bran are at least one, and 5% corn flour, 3% bean cake powder and soybean powder are at least one, 0.5% white sugar, 0.5% gypsum, 1% stone Ash calculates the weight of each material according to dress packet quantity according to above-mentioned formula, weighs up, carries out mixing spice, and adjusting pH value is 6., Start to carry out after stirring evenly to add water, make water content 70%, adjusting PH is 9;
(2) it builds heap fermentation: uniformly mixed raw material is piled into wide 1.0m, the material heap of high 1.0m, building after heap with diameter is 5cm Wooden stick prick upper vent hole in material heap, every 30cm inserts a hole, in Chinese character pin-shaped between Kong Yukong, in favor of aerobic fementation, carries out Overlay film moisturizing carries out turning when material heap reaches 60 DEG C, and whole fermentation process is 7 days, whole turning 3 times, after fermentation, will Material heap unplugs, and shakeouts cooling, exhaust gas to be discharged, the production of blanket type bacterium bed can be carried out when material temperature is down to 20 DEG C or so.
(3) production of blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is by strain With bacterium bed matrix made of compost, third layer is PE leaching membrane paper;The size of bacterium bed is width 90cm, length 2m;
(4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 90cm in carrier chain upper berth, on non-woven fabrics upper berth The Dictyophora rubrovalvata strain of 1cm thickness, and strain is flattened with stainless steel platen machine, then the hair of 2cm thickness is repaved in the bacterium bed of strain Ferment material, and flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1cm thickness that tiles again on the compost of pressing is simultaneously compacted with trigger squeeze, pressure It is flat;
(5) one layer of PE leaching membrane paper, and the bacterium bed that will be made with beamer will be covered above the bacterium bed flattened in step (4) It all rolls and is put into culturing room's progress bacterium germination;
(6) culture room temperature is set as 22 DEG C, and humidity 60%, gas concentration lwevel < 5000ppm is cultivated under this culture Dictyophora rubrovalvata blanket type bacterium bed can be formed within 30 days, can be used to soil fruiting after bacterium germination.
A kind of production method of the Dictyophora rubrovalvata blanket type bacterium bed of embodiment 2
(1) blanket type bacterium bed raw material disposal, by weight percentage, by 55% weed tree sawdust, 15% cotton seed hulls, 20% meter Chaff and wheat bran are at least one, 5% corn flour, and 2.5% bean cake powder and soybean powder are at least one, 1% white sugar, 1% gypsum, and 0.5% Lime calculates the weight of each material according to dress packet quantity according to above-mentioned formula, weighs up, carries out mixing spice, adjusts pH value and is 65, start to carry out after stirring evenly to add water, make water content 73%, adjusting PH is 9.5;
(2) it builds heap fermentation: uniformly mixed raw material is piled into wide 1.2m, the material heap of high 1.1m, building after heap with diameter is 6cm Wooden stick prick upper vent hole in material heap, every 32cm inserts a hole, in Chinese character pin-shaped between Kong Yukong, in favor of aerobic fementation, carries out Overlay film moisturizing carries out turning when material heap reaches 65 DEG C, and whole fermentation process is 8 days, whole turning 4 times, after fermentation, will Material heap unplugs, and shakeouts cooling, exhaust gas to be discharged, the production of blanket type bacterium bed can be carried out when material temperature is down to 20 DEG C or so.
(3) production of blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is by strain With bacterium bed matrix made of compost, third layer is PE leaching membrane paper;The size of bacterium bed is but not limited to this wide 100cm, length 2.5m;
(4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 100cm in carrier chain upper berth, on non-woven fabrics upper berth The Dictyophora rubrovalvata strain of 1.2cm thickness, and strain is flattened with stainless steel platen machine, then 2.5cm thickness is repaved in the bacterium bed of strain Fermentation material, and flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1-1.5cm thickness that tiles again on the compost of pressing simultaneously uses trigger squeeze Compacting flattens;
(5) one layer of PE leaching membrane paper, and the bacterium bed that will be made with beamer will be covered above the bacterium bed flattened in step (4) It all rolls and is put into culturing room's progress bacterium germination;
(6) culture room temperature is set as 24 DEG C, and humidity 65%, gas concentration lwevel < 4000ppm is cultivated under this culture Dictyophora rubrovalvata blanket type bacterium bed can be formed within 35 days, can be used to soil fruiting after bacterium germination.
A kind of production method of the Dictyophora rubrovalvata blanket type bacterium bed of embodiment 3
(1) blanket type bacterium bed raw material disposal, by weight percentage, by 60% weed tree sawdust, 20% cotton seed hulls, 10% meter Chaff and wheat bran at least one, 4% corn flour, 3% bean cake powder and soybean powder at least one, 1% white sugar, 1% gypsum, 1% lime, The weight for calculating each material according to dress packet quantity according to above-mentioned formula, weighs up, carries out mixing spice, and adjusting pH value is 7.0, stirs Start to carry out after mixing uniformly to add water, make water content 70-76%, adjusting PH is 10;
(2) it builds heap fermentation: uniformly mixed raw material is piled into wide 1.5m, the material heap of high 1.2m, building after heap with diameter is 8cm Wooden stick prick upper vent hole in material heap, every 35cm inserts a hole, in Chinese character pin-shaped between Kong Yukong, in favor of aerobic fementation, carries out Overlay film moisturizing carries out turning when material heap reaches 70 DEG C, and whole fermentation process is 10 days, whole turning 5 times, after fermentation, Material heap is unpluged, cooling, exhaust gas to be discharged is shakeout, the production of blanket type bacterium bed can be carried out when material temperature is down to 20 DEG C or so.
(3) production of blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is by strain With bacterium bed matrix made of compost, third layer is PE leaching membrane paper;The size of bacterium bed is but not limited to this wide 120cm, length 3m;
(4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 120cm in carrier chain upper berth, on non-woven fabrics upper berth The Dictyophora rubrovalvata strain of 1.5cm thickness, and strain is flattened with stainless steel platen machine, then 3cm thickness is repaved in the bacterium bed of strain Fermentation material, and being flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1.5cm thickness that tiles again on the compost of pressing and with trigger squeeze pressure It is real, it flattens;
(5) one layer of PE leaching membrane paper, and the bacterium bed that will be made with beamer will be covered above the bacterium bed flattened in step (4) It all rolls and is put into culturing room's progress bacterium germination;
(6) culture room temperature is set as 26 DEG C, and humidity 70%, gas concentration lwevel < 3000ppm is cultivated under this culture Dictyophora rubrovalvata blanket type bacterium bed can be formed within 40 days, can be used to soil fruiting after bacterium germination.

Claims (7)

1. a kind of production method of Dictyophora rubrovalvata blanket type bacterium bed, which comprises the following steps:
(1) blanket type bacterium bed raw material disposal, by weight percentage, by 50-60% weed tree sawdust, 10-20% cotton seed hulls, 10- 30% rice bran and wheat bran are at least one, 3-5% corn flour, and 2-3% bean cake powder and soybean powder are at least one, 0.5-1% white sugar, 0.5-1% gypsum, 0.5-1% lime calculate the weight of each material according to dress packet quantity according to above-mentioned formula, weigh up, carries out Spice is mixed, adjusting pH value is 6.0-7.0, starts to carry out after stirring evenly to add water, makes water content 70-76%, adjusts PH For 9-10;
(2) it builds heap fermentation: uniformly mixed raw material being piled into wide 1.0-1.5m, the material heap of high 1.0-1.2m uses diameter after building heap Upper vent hole is pricked in material heap for the wooden stick of 5-8cm, and every 30-35cm inserts a hole, in Chinese character pin-shaped between Kong Yukong, in favor of ventilation Fermentation carries out overlay film moisturizing, and turning is carried out when material heap reaches 60-70 DEG C, and whole fermentation process is 7-10 days, and high temperature season turns over Heap turning in 1-2 days 1 time, low temperature season turning in 2-3 days 1 time, whole turning 3-5 times after fermentation unplugs material heap, shakeouts Cooling, exhaust gas to be discharged can carry out the production of blanket type bacterium bed when material temperature is down to 20 DEG C or so.
(3) production of blanket type bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is by strain and training Bacterium bed matrix made of nutriment, third layer are PE leaching membrane paper;
(4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 90-120cm in carrier chain upper berth, on non-woven fabrics upper berth The Dictyophora rubrovalvata strain of 1-1.5cm thickness, and strain is flattened with stainless steel platen machine, then repave 2-3cm in the bacterium bed of strain Thick fermentation material, and flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1-1.5cm thickness that tiles again on the compost of pressing simultaneously uses pressing plate Machine compacting, flattens;
(5) one layer of PE leaching membrane paper will be covered above the middle bacterium bed flattened of step (4), and with beamer that the bacterium bed made is whole It rolls and is put into culturing room's progress bacterium germination;
(6) culture room temperature is set as 22-26 DEG C, humidity 60-70%, and gas concentration lwevel < 5000ppm is trained under this culture Dictyophora rubrovalvata blanket type bacterium bed can be formed by supporting 30-40 days, can be used to soil fruiting after bacterium germination.
2. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: the step (1) blanket type bacterium bed raw material disposal, by weight percentage, by 55% weed tree sawdust, 15% cotton seed hulls, 20% rice bran and wheat bran At least one, 5% corn flour, 2.5% bean cake powder and soybean powder are at least one, 1% white sugar, 1% gypsum, 0.5% lime, according to Above-mentioned formula calculates the weight of each material according to dress packet quantity, weighs up, and carries out mixing spice, and adjusting pH value is 65, and stirring is equal Start to carry out after even to add water, make water content 73%, adjusting PH is 9.5.
3. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: the step (2) it builds heap fermentation: uniformly mixed raw material is piled into wide 1.0-1.5m, the material heap of high 1.0-1.2m, building after heap with diameter is 5- The wooden stick of 8cm pricks upper vent hole in material heap, and every 30-35cm inserts a hole, in Chinese character pin-shaped between Kong Yukong, in favor of ventilation hair Ferment carries out overlay film moisturizing, and turning is carried out when material heap reaches 65 DEG C, and whole fermentation process is 8 days, high temperature season turning 1-2 days Turning 1 time, after fermentation, material heap is unpluged, cooling is shakeout, wait arrange for low temperature season turning in 2-3 days 1 time, whole turning 4 times Exhaust gas out can carry out the production of blanket type bacterium bed when material temperature is down to 20 DEG C or so.
4. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: (3) The production of blanket bacterium bed: by up of three-layer, the bottom is the non-woven fabrics to have sterilized, and the second layer is made of strain and compost Bacterium bed matrix, third layer are PE leaching membrane paper;The size of bacterium bed is width 100cm, length 2.5m.
5. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: the step (4) according to the blanket type bacterium bed in step (3), in the non-woven fabrics of the wide 100cm in carrier chain upper berth, on non-woven fabrics upper berth, 1.2cm is thick Dictyophora rubrovalvata strain, and strain is flattened with stainless steel platen machine, then the fermentation of 2.5cm thickness is repaved in the bacterium bed of strain Material, and flattened with pressing plate, the Dictyophora rubrovalvata strain for the 1.2cm thickness that tiles again on the compost of pressing is simultaneously compacted with trigger squeeze, pressure It is flat;The PE leaching membrane paper for finally repaving a slice width 100cm plays moisturizing, ventilative effect;And spooled with beamer, it is put into In culturing room.
6. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: the step (6) culture room temperature is set as 23-25 DEG C, humidity 63-67%, and gas concentration lwevel < 4000ppm cultivates 30- under this culture Dictyophora rubrovalvata blanket type bacterium bed can be formed within 40 days, can be used to soil fruiting after bacterium germination.
7. the production method of Dictyophora rubrovalvata blanket type bacterium bed according to claim 1, it is characterised in that: the step (6) culture room temperature is set as 24 DEG C, humidity 65%, gas concentration lwevel < 3000ppm, cultivates 30-40 days under this culture and is Dictyophora rubrovalvata blanket type bacterium bed can be formed, can be used to soil fruiting after bacterium germination.
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CN112640730A (en) * 2021-01-26 2021-04-13 贵州丰源现代农业有限公司 High-yield dictyophora rubrovolvata fungus bag and preparation method and application thereof
CN112931048A (en) * 2021-03-02 2021-06-11 贵州丰源现代农业有限公司 Dictyophora rubrovalvata fungus bag and cultivation method of Dictyophora rubrovalvata

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