CN102919055A

CN102919055A - Efficient seed production method of dictyophora rubrovolvata

Info

Publication number: CN102919055A
Application number: CN2012104574379A
Authority: CN
Inventors: 杨森; 杨胜波; 吴安祥
Original assignee: KAILI SENCHENG PLANTATION
Current assignee: KAILI SENCHENG PLANTATION
Priority date: 2012-11-15
Filing date: 2012-11-15
Publication date: 2013-02-13

Abstract

The invention discloses an efficient seed production method of dictyophora rubrovolvata. The method comprises the following operating steps: 1) fermenting windrow to nourishment; 2) putting the fermented nourishment into a fungus bag; 3) inoculating strains on multiple points on the upper part, the middle part and the lower part of the fungus bag during the process for putting the fungus bag; and 4) cultivating the strains into a culture room. By adopting the method, the seed production period and the mycelial growth time are effectively reduced, and the seed production period is reduced to 5 months from one and a half years; the mycelial growth is reduced to 60 days from 180 days; the original nourishment is steamed at high temperature before fermentation, and the strain yield is 70%, the strain yield in the method is improved to be more than 98%, the seed production cost is saved, and the production cost for one kilogram of strain is reduced to 1.2 yuan from 5.6 yuan. According to the method, medicine injury and ultraviolet radioactivity injury on the human body due to sterilization operation can be reduced, and fitness of a seed production operator is effectively protected.

Description

The breeding high efficiency seed method of a kind of Dictyophora rubrovalvata bacterium

Technical field

The present invention relates to the breeding high efficiency seed method of a kind of Dictyophora rubrovalvata bacterium.

Background technology

It is to carry out high-temperature steam sterilization with the vial charging that prior art is produced Dictyophora rubrovalvata bacterial classification method, after the cooling, under the environmental condition of sealing, uses mechanical filter in pharmaceutical aerosol sterilization or ozone sterilization, the air, in aseptic area bacterial classification is accessed in the bottle; Each link must be carried out under aseptic technique, and the aseptic technique requirement is very strict, careless slightly, will bring miscellaneous bacteria to infect and suffer a loss.

The said method working procedure is very complicated, workload is large especially, and the equipment and the drug variety that relate to are many, and the bacterial classification production cost is high, the cultural hypha growth period is slow after the inoculation, and the whole cycle of Dictyophora rubrovalvata bacterium from the production first class inoculum to three-class strain needs time a year and a half; Because mycelial growth is slow, incubation time is long, cause the bacteria infection probability high, at the bottom of the position mycelia is not covered with bottle under the bacterial classification in the bottle, the bacterial classification on top just begins to wear out, even atrophy, reduces to some extent strain quality, the bacterial classification yield rate generally about 70%, seriously affects and has restricted the raising of Dictyophora rubrovalvata cultivation output.

Summary of the invention

For the deficiencies in the prior art part, the invention provides the breeding high efficiency seed method of a kind of Dictyophora rubrovalvata bacterium, guaranteeing effectively to shorten the production of hybrid seeds cycle of Dictyophora rubrovalvata bacterium, and improve the bacterial classification yield rate.

The technical solution used in the present invention: the breeding high efficiency seed method of a kind of Dictyophora rubrovalvata bacterium comprises following operating procedure:

1 in the following component of the indoor employing of Edible Fungi by the quality proportioning, the rear windrow fermentation that stirs is fuel, in the windrow fermentation process, 1-7 days temperature is 65-75 ℃, 8-18 days temperature is 45-55 ℃,

Dried wood chip 65-75 corncobs 8-12 wheat skins 13-17 land plasters 0.8-1 oil cake 0.8-1.2 chicken manures 0.8-1.2 potassium dihydrogen phosphates 0.18-0.22 magnesium sulfate 0.2-0.22 running water 65-72;

The 2 above-mentioned fuels that will the ferment bacterium pocket of packing into, each bacterium pocket is loaded onto and is stated bacterium material 0.65-0.7 kilogram;

In the 3 dress bacterium pocket processes, the bacterium pocket lower, in, upper part multiple spot access bacterial classification, frap bacterium pocket mouth, and in time access bacterial classification place with toothpick at each bacterium Bag Material and make a call to an air-vent, the bacterium pocket changes culturing room over to and carries out cultural hypha;

4 in culturing room, in piling bin lower floor the bacterium pocket is fallen down to pile up and put 7, in the piling bin middle level bacterium pocket fallen down to pile up and put 5, on the piling bin upper strata bacterium pocket fallen down to pile up and put 5, the temperature of culturing room is 20-22 ℃, and air humidity requires between the 65%-70%, watered with sprayer every 2-3 days, culturing room keeps lightproof ventilated, and mycelia was covered with the bacterium pocket in 55-62 days, can provide to produce cultivation with planting.

Adopt said method effectively to shorten production of hybrid seeds cycle and mycelial growth time, the production of hybrid seeds cycle shortens to 5 months by original a year and a half; Mycelial growth shortened to 60 days by original 180 days; Fuel originally needs high temperature to steam before fermentation; and the bacterial classification yield rate is 70%; the bacterial classification yield rate of this method is brought up to more than 98%; save simultaneously production of hybrid seeds production cost; need 5.6 yuan by one kilogram of bacterial classification production cost of original production and be reduced to 1.2 yuan; this method also reduces drug damages and the ultraviolet radiation injury that disinfecting action brings human body, protects effectively that production of hybrid seeds operating personnel's is healthy.

Embodiment

Place: the gloomy one-tenth of Kaili City plantation.

1 is fuel in the stir windrow fermentation of the following component of the indoor employing of Edible Fungi, and in the windrow fermentation process, 1-7 days temperature is 65-75 ℃, and 8-18 days temperature is 45-55 ℃;

65 kilograms in 0.2 kilogram of running water of dried 1 kilogram of chicken manure of 1 kilogram of oil cake of 15 kilograms of land plasters of 10 kilograms of wheat skins of wood chip 70 kg corn cores, 0.8 kilogram of potassium dihydrogen phosphate, 0.2 kilo sulfuric acid magnesium;

In 3 each bacterium pocket pack process, the bacterium pocket lower, in, upper part multiple spot access bacterial classification, frap bacterium pocket mouth, and in time access bacterial classification place with toothpick at each bacterium pocket and make a call to an air-vent, then the bacterium pocket changes culturing room over to and carries out cultural hypha;

4 evenly stack bacterium pocket kind on the piling bin of culturing room, because in the process of packing inoculation, the probio in the composts or fertilisers of cultivating is also continuing survival, carries out the Growth and reproduction of self, so still can produce certain heat in the bacterium pocket stacking process, it is thick that the bacterium pocket should not be stacked Tai Gaotai; In piling bin lower floor the bacterium pocket is fallen down to pile up and put 7, in the piling bin middle level bacterium pocket fallen down to pile up and put 5, on the piling bin upper strata bacterium pocket fallen down to pile up and put 5, surpass 30 and burn the dictyophora phalloidea mycelia when spending to death so that fungi-proofing pocket temperature is too high; The temperature of culturing room is 20-22 ℃; Between the air humidity requirement 65%-70%, watered with sprayer every 2-3 days, culturing room keeps lightproof ventilated, and mycelia was covered with the bacterium pocket in 60 days, can provide and produce cultivation with planting.

Claims

1. the breeding high efficiency seed method of a Dictyophora rubrovalvata bacterium is characterized in that comprising following operating procedure:

(1) press the quality proportioning in the following component of the indoor employing of Edible Fungi, the rear windrow fermentation that stirs is fuel, and in the windrow fermentation process, 1-7 days temperature is 65-75 ℃, and 8-18 days temperature is 45-55 ℃,

The above-mentioned fuel that (2) will the ferment bacterium pocket of packing into, each bacterium pocket is loaded onto and is stated bacterium material 0.65-0.7 kilogram;

(3) in the dress bacterium pocket process, the bacterium pocket lower, in, upper part multiple spot access bacterial classification, frap bacterium pocket mouth, and in time access bacterial classification place with toothpick at each bacterium Bag Material and make a call to an air-vent, the bacterium pocket changes culturing room over to and carries out cultural hypha;

(4) in culturing room, in piling bin lower floor the bacterium pocket is fallen down to pile up and put 7, in the piling bin middle level bacterium pocket fallen down to pile up and put 5, on the piling bin upper strata bacterium pocket fallen down to pile up and put 5, the temperature of culturing room is 20-22 ℃, and air humidity requires between the 65%-70%, watered with sprayer every 2-3 days, culturing room keeps lightproof ventilated, and mycelia was covered with the bacterium pocket in 55-62 days, can provide to produce cultivation with planting.