CN105532261A

CN105532261A - Method for shortening strain production cycle of Dictyophora indusiata

Info

Publication number: CN105532261A
Application number: CN201510935596.9A
Authority: CN
Inventors: 廖明武
Original assignee: Guizhou Qiankang Ecological Technology Agriculture Development Co Ltd
Current assignee: Guizhou Qiankang Ecological Technology Agriculture Development Co Ltd
Priority date: 2015-12-15
Filing date: 2015-12-15
Publication date: 2016-05-04

Abstract

The invention discloses a method for shortening a strain production cycle of Dictyophora indusiata. The method includes the following steps: 1, allowing piled materials to be fermented to be served as a nutritive material; 2, putting the fermented nutritive material into a fungus bag; 3, putting strains into the upper, center, lower positions of the fungus bag; and 4, culturing the strains in a culture room. Through the method, the strain production cycle and the hypha growth time can be effectively shortened, and the strain production cycle is reduced from 1.5 years for a conventional method to 6 months; according to the conventional method, a nutritive material needs high temperature steam before fermentation and the yield of the strains is 70%, and the yield of the strains of the provided method can be increased over 95%; the strain production cost can be saved; through the method, drug harm and ultraviolet radiation harm to a human body due to sterilizing operation can be reduced, and the health of an strain production operator can be effectively protected.

Description

A kind of method shortening the dictyophora phalloidea fungus preparing seeds cycle

Technical field

The present invention relates to a kind of method shortening the dictyophora phalloidea fungus preparing seeds cycle.

Background technology

It is carry out high-temperature steam sterilization with vial charging that prior art produces dictyophora phalloidea bacterial classification method, after cooling, under the environmental condition closed, uses mechanical filter in pharmaceutical aerosol sterilization or ozone sterilization, air, accesses in bottle in aseptic area by bacterial classification; Each link must be carried out under aseptic technique, and aseptic technique requires very strict, careless slightly, miscellaneous bacteria will be brought to infect and suffer a loss.

Said method working procedure is very complicated, and workload is large especially, the equipment related to and drug variety many, bacterial classification production cost is high, and after inoculation, cultural hypha growth period is slow, and dictyophora phalloidea bacterium needs time a year and a half from producing the whole cycle of first class inoculum to three-class strain; Because mycelial growth is slow, incubation time is long, cause bacteria infection probability high, at the bottom of in bottle, bacterial classification lower portion mycelia is not covered with bottle, the bacterial classification on top just starts aging, and even atrophy, reduces strain quality to some extent, bacterial classification yield rate, generally about 70%, seriously affects and constrains the raising of Dictyophora rubrovalvata cultivation yield.

Summary of the invention

For the deficiencies in the prior art part, the invention provides a kind of method shortening the dictyophora phalloidea fungus preparing seeds cycle, to guarantee the production of hybrid seeds cycle effectively shortening dictyophora phalloidea bacterium, and improve bacterial classification yield rate.

The technical solution used in the present invention: a kind of method shortening the dictyophora phalloidea fungus preparing seeds cycle, comprises following operating procedure:

1 presses quality proportioning at the indoor employing following component of Edible Fungi, and after stirring, windrow fermentation is fuel, and in windrow fermentation process, the temperature of 1-20 days is 60-65 DEG C,

Dry wood chip 65-75 corncob 8-12 rice bran 13-17 land plaster 0.8-1 vegetable seed oil cake 0.8-1.2 chicken manure 0.8-1.2 running water 65-72;

The above-mentioned fuel fermented is loaded bacterium pocket by 2, and each bacterium pocket is loaded onto and stated bacterium material 0.5-0.6 kilogram;

In 3 dress bacterium pocket processes, under bacterium pocket, in, on position multiple spot access bacterial classification, frap bacterium pocket mouth, and timely toothpick accesses bacterial classification place at each bacterium Bag Material makes a call to an air-vent, bacterium pocket proceeds to culturing room and carries out cultural hypha;

4 in culturing room, in piling bin lower floor bacterium pocket fallen down to pile up and put 8, in piling bin middle level, bacterium pocket is fallen down to pile up and put 5, fall down to pile up by bacterium pocket on piling bin upper strata and put 5, the temperature of culturing room is 20-22 DEG C, and air humidity requires between 65%-70%, watered with sprayer every 1-2 days, culturing room keeps lightproof ventilated, and within 50-60 days, mycelia covers with bacterium pocket, can provide and produce cultivation kind.

Adopt said method effectively to shorten production of hybrid seeds cycle and mycelial growth time, the production of hybrid seeds cycle shortens to 6 months by original a year and a half; Fuel originally needs high temperature to steam before fermentation, and bacterial classification yield rate is 70%, and the bacterial classification yield rate of this method brings up to more than 95%.

Embodiment

Border of the county, Shibing is in the Yunnan-Guizhou Plateau second step to the excessive area of the East Hillside Plain first step, and whole physical features northwest is higher, and the southeast is lower, mean sea level about 800 meters, and belong to monsoon moist climate region, subtropics, warmer climate is moistening.Be suitable for dictyophora phalloidea bacterial classification to plant.

1 stirs windrow fermentation for fuel at the indoor employing following component of Edible Fungi, and in windrow fermentation process, the temperature of 1-7 days is 65-75 DEG C, and the temperature of 8-18 days is 45-55 DEG C;

65 kilograms, dry wood chip 65 kg corn core 10 kilograms of rice brans 15 kilograms of land plasters 0.8 kilogram of vegetable seed oil cake 1 kilogram of chicken manure 0.8 kilogram of running water;

The above-mentioned fuel fermented is loaded bacterium pocket by 2, and each bacterium pocket is loaded onto and stated bacterium material 0.5-0.7 kilogram;

In 3 each bacterium pocket bagging process, under bacterium pocket, in, on position multiple spot access bacterial classification, frap bacterium pocket mouth, and timely toothpick accesses bacterial classification place at each bacterium pocket makes a call to an air-vent, then bacterium pocket proceeds to culturing room and carries out cultural hypha;

Bacterium pocket kind is evenly stacked by 4 on the piling bin of culturing room, in process due to packaging inoculation, the probio in composts or fertilisers of cultivating also in continuation survival, carries out the Growth and reproduction of self, so still can produce certain heat in bacterium pocket stacking process, it is thick that bacterium pocket should not stack Tai Gaotai; In piling bin lower floor bacterium pocket fallen down to pile up and puts 8, in piling bin middle level, bacterium pocket is fallen down to pile up and put 5, on piling bin upper strata, bacterium pocket is fallen down to pile up and put 5, with fungi-proofing pocket temperature too high more than 30 degree time burn dictyophora phalloidea mycelia to death; The temperature of culturing room is 20-22 DEG C; Air humidity requires, between 65%-70%, to water with sprayer every 1-2 days, and culturing room keeps lightproof ventilated, and within 60 days, mycelia covers with bacterium pocket, can provide and produce cultivation kind.

Claims

1. shorten the method in dictyophora phalloidea fungus preparing seeds cycle, it is characterized in that comprising following operating procedure:

1) press quality proportioning at the indoor employing following component of Edible Fungi, after stirring, windrow fermentation is fuel, and in windrow fermentation process, the temperature of 1-20 days is 60-65 DEG C,

2) the above-mentioned fuel fermented is loaded bacterium pocket, each bacterium pocket is loaded onto and is stated bacterium material 0.5-0.6 kilogram;

3) fill in bacterium pocket process, under bacterium pocket, in, on position multiple spot access bacterial classification, frap bacterium pocket mouth, and timely toothpick accesses bacterial classification place at each bacterium Bag Material makes a call to an air-vent, bacterium pocket proceeds to culturing room and carries out cultural hypha;

4) in culturing room, in piling bin lower floor bacterium pocket fallen down to pile up and put 8, in piling bin middle level, bacterium pocket is fallen down to pile up and put 5, fall down to pile up by bacterium pocket on piling bin upper strata and put 5, the temperature of culturing room is 20-22 DEG C, and air humidity requires between 65%-70%, watered with sprayer every 1-2 days, culturing room keeps lightproof ventilated, and within 50-60 days, mycelia covers with bacterium pocket, can provide and produce cultivation kind.