CN204814273U - Microcirculation of microscope mesenterium is observed with carrying thing device - Google Patents
Microcirculation of microscope mesenterium is observed with carrying thing device Download PDFInfo
- Publication number
- CN204814273U CN204814273U CN201520538021.9U CN201520538021U CN204814273U CN 204814273 U CN204814273 U CN 204814273U CN 201520538021 U CN201520538021 U CN 201520538021U CN 204814273 U CN204814273 U CN 204814273U
- Authority
- CN
- China
- Prior art keywords
- observation
- microscope
- area
- arranges
- observation area
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model provides a microcirculation of microscope mesenterium is observed with carrying thing device, including perfusion box, fixed plate and temperature control device. The utility model discloses small, constant temperature and humidity, be convenient for keep the mesenterium sample clean, can firmly fix experimental animals and just closely cooperate with the microscope.
Description
Technical field
This utility model belongs to medical microscopic viewing instrument field, is specifically related to a kind of microscope Mesentery microcirculation observation carrying apparatus.
Background technology
Microcirculation district is the place that mass exchange is directly carried out in blood and tissue fluid, and its blood circumstance can be observed by means of microscope.Organizing of mesentery position is thin, is suitable for easypro contracting and the blood circumstance of directly observing microcirculation district blood vessel.
The constant temperature perfusion device volume that existing Mesentery microcirculation is observed is larger, match with the microcirculation microscope of selling in the market, because volume is greatly not easily laid, can not at common biological microscope, especially the high-end microscope such as Bu Laide multinomial projection microscope system uses, have impact on the fineness of Microcirculatory Observations.The microcirculation microscope resolution of selling in the market is less, function singleness, is mainly used in the gross examination of microcirculation form, fluidised form and surrounding shape body, can not carries out more deep, careful observational study to microcirculation cytorheology.Cloth Rider multinomial projection microscope system and part special biological microscope resolution high, the former has the multiple optical function such as difference, dark ground, bright field, polarisation, Midst density bright field, continuous videos amplification, except to except the conventional microcirculation inspections such as microcirculation form, fluidised form, ambient state, also the rheological behaviour of the erythrocyte in microcirculation cytorheology, platelet, leukocyte, blood plasma is observed and quantitative analysis, but in prior art, also do not occur the device of the animal intestine mesentery Microcirculatory Observations supporting with it.
Chinese patent " rat mesenteria microcirculation observation constant-temperature perfusion device " (patent No. ZL2005201441671) provides a kind of constant temperature perfusion device for observation experiment, but this device observes site can not realize constant temperature and humidity, and this device is provided with the structures such as sliding door, device overall volume is excessive, not easily be placed on microscopic examination platform, connection that can not be closely firm with microscope.
Utility model content
This utility model provides that a kind of volume is little, constant temperature and humidity, be convenient to keep mesentery sample clean, can fixedly secure to connect closely between laboratory animal, parts and with microscope close-fitting Mesentery microcirculation observation carrying apparatus.
The technical scheme that this utility model solves the employing of its technical problem is: a kind of microscope Mesentery microcirculation observation carrying apparatus, comprises perfusion box, fixing head and temperature control equipment;
Described perfusion box comprises observation area, the thermal treatment zone and clear area, arranges Microcirculatory Observations platform in described observation area, and described Microcirculatory Observations platform higher than the base plate place plane of observation area and printing opacity, arranges temperature-sensing probe in described observation area; Arrange electrothermal tube in the described thermal treatment zone, the described thermal treatment zone is communicated with observation area;
The bolt described fixing head being arranged more than one screw and coordinate with described screw, also arranges the observation draw-in groove of holding in observation platform outer;
Described temperature control equipment is connected by wire with electrothermal tube and temperature-sensing probe.
Preferably, connectivity slot or intercommunicating pore are set between the thermal treatment zone and observation area.
Preferably, described perfusion box also comprises the fixed area be arranged on outside it, and the outer of described fixed area arranges the holddown groove mated with microscope carrier sample slice clamper fixing threaded hole.
Preferably, the opposite edge that draw-in groove observed by described fixing head arranges the fixed card slot mated with microscope handel.
Preferably, described Microcirculatory Observations platform is fixedly connected with the base plate of observation area by joint pin, and described joint pin is ground glass material.
Preferably, described perfusion box arranges temperature controlled region, and electrothermal tube is connected with temperature controller through temperature controlled region with the wire of temperature-sensing probe, and avoiding the junction point of wire and electrothermal tube and temperature probe to expose affects service life in atmosphere.
The model that temperature control equipment described in the utility model selects Kai Hua Intelligent Instrument Watch Factory to produce is TDA8002 type temperature controller.
Described observation platform is the good glass of light transmission or resin, for the light source through microscopic examination hole.
The beneficial effects of the utility model are:
(1) electrothermal tube, temperature-sensing probe and temperature control equipment is utilized to keep the temperature and humidity in perfusion device constant;
(2) volume is little, is conveniently placed on microscopical observation platform, is easy to mobile, flexible operation;
(3) the observation draw-in groove on fixing head is realized and being connected of perfusion box in the outer of joint pin by holding, and connect and closely stablize, fixing head covers the evaporation that can reduce solution in perfusion box above perfusion box;
(4) Microcirculatory Observations platform is transparent light-transmitting materials, and joint pin is frosted material, and the light in microscopic examination hole effectively can pass through Microcirculatory Observations platform, and joint pin reduces loss of light source, improves the definition observed;
(5) perfusion box and fixing head all arrange the groove with microscopes, with microscope stable connection;
(6) clear area provides the storage space of cleanout fluid, conveniently in experimentation, draws clean solution from clear area, and cleaning mesentery tissue, improves the definition of tissue;
(7) when being communicated with intercommunicating pore between the thermal treatment zone and observation area, intercommunicating pore can filter the foreign material of observation area, prevents it from entering the thermal treatment zone, affects the normal work of heating element heater.
This utility model microscope Mesentery microcirculation observation carrying apparatus, volume is little, and space layout utilizes rationally, can effectively reach constant temperature and humidity effect, be specially adapted to microscopic examination animal Mesentery microcirculation, have a good application prospect.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1;
Fig. 2 is the structural representation of embodiment 1 perfusion box;
Fig. 3 is the structural representation of embodiment 1 fixing head;
Fig. 4 is the structural representation of embodiment 2 perfusion box;
Fig. 5 is the structural representation of embodiment 3 perfusion box.
In accompanying drawing, 1 perfusion box, 2 fixing heads, 3 temperature control equipments, 4 observation areas, 5 thermals treatment zone, 6 clear areas, 7 Microcirculatory Observations platforms, 8 temperature-sensing probes, 9 electrothermal tubes, 10 screws, 11 observe draw-in groove, 12 fixed areas, 13 holddown grooves, 14 fixed card slots, 15 joint pins, 16 bolts, 17 connectivity slots, 18 temperature controlled region, 19 intercommunicating pores.
Detailed description of the invention
For enabling above-mentioned purpose of the present utility model, feature and advantage become apparent more, below in conjunction with the drawings and specific embodiments in this utility model embodiment, technical scheme in this utility model embodiment is clearly and completely described, obviously, described embodiment is only this utility model part embodiment, instead of whole embodiments.Based on the embodiment in this utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of this utility model protection.
embodiment 1
As Figure 1-3, embodiment 1 comprises perfusion box 1, fixing head 2 and temperature control equipment 3;
Described perfusion box 1 comprises observation area 4, the thermal treatment zone 5, clear area 6, fixed area 12 and temperature controlled region 18, and described fixed area 12 is arranged on the side of the thermal treatment zone 5, and clear area 6 and fixed area 12 are arranged on the opposite side of the thermal treatment zone 5;
Temperature-sensing probe 8 and Microcirculatory Observations platform 7 are set in described observation area 4, described Microcirculatory Observations platform 7 is higher than observation area 4 place plane and printing opacity, described Microcirculatory Observations platform 7 is fixedly connected with by the base plate of joint pin 15 with observation area 4, and described joint pin 15 is ground glass material; Arrange electrothermal tube 9 in the described thermal treatment zone 5, the described thermal treatment zone 5 is communicated with observation area 4, arranges connectivity slot 17 between the thermal treatment zone 5 and observation area 4; The outer of described fixed area 12 arranges the holddown groove 13 mated with microscope carrier sample slice clamper fixing threaded hole.
The bolt 16 described fixing head 2 being arranged more than one screw 10 and coordinate with described screw 10, described fixing head 2 side is provided with the observation draw-in groove 11 of holding in Microcirculatory Observations platform 7 outer, opposite side arranges the fixed card slot 14 mated with microscope handel, the shape of described observation draw-in groove 11 and size and joint pin 15 outer shape, consistent size;
Described temperature control equipment 3 is connected by wire with electrothermal tube 9 and temperature-sensing probe 8, and the model that described temperature control equipment 3 selects Kai Hua Intelligent Instrument Watch Factory to produce is TDA8002 type temperature controller.
Described Microcirculatory Observations platform 7 is the good glass of light transmission, for the light source through microscopic examination hole.
Electrothermal tube 9 is connected with temperature controller through temperature controlled region 18 with the wire of temperature-sensing probe 8, and avoiding the junction point of wire and electrothermal tube 9 and temperature probe 8 to expose affects service life in atmosphere.
Embodiment 1, for microexamination animal Mesentery microcirculation, is specially adapted to the Microcirculatory Observations that the multinomial microscopic system of Bu Laide carries out.During use, be placed on by perfusion box 1 on microscopical object stage, the holddown groove 13 of fixed area 12 is fastened in outside microscope carrier sample slice clamper fixing threaded hole, Microcirculatory Observations platform 7 aligming microscope objective lens.In the thermal treatment zone 5, observation area 4 and clear area 6, add normal saline, open temperature control equipment 3, electrothermal tube 9 pairs of normal saline heat.
Laboratory animal is fixed on fixing head 2, with fixing band, animal is bound, and utilized by fixing band the screw 10 on fixing head 2 and bolt 16 to fix.Be placed on by fixing head 2 above perfusion box 1, fixed card slot 14 is stuck on microscope handel, observes draw-in groove 11 holding in joint pin 15 outer.
Be placed on by the mesentery of laboratory animal on Microcirculatory Observations platform 7, clean with the normal saline flushing of clear area 6, the normal saline of the thermal treatment zone 5 and observation area 4 provides the environment of constant temperature and humidity for mesentery tissue, keeps the concordance inside and outside organizer and stability.Microcirculatory Observations platform 7 is the good light-transmitting materials of light transmission, is convenient to microscope light source and passes, and improves the definition observed.
embodiment 2
As shown in Figure 4, composition graphs 1-3, the fixed area 12 of embodiment 1 perfusion box 1 and the thermal treatment zone 5 in the same side of observation area 4, the fixed area 12 of embodiment 2 with the thermal treatment zone 5 respectively in the relative both sides of observation area 4; The fixing head 2 of embodiment 1 is provided with fixed card slot 14, and embodiment 2 does not arrange fixed card slot 14.
Embodiment 2 be applicable to laboratory animal volume larger time, fixing head 2 is not easily placed between microscope objective and the object stage of handel.During use, be placed on by perfusion box 1 on microscopical object stage, the holddown groove 13 of fixed area 12 is fastened in microscope carrier sample slice clamper fixing threaded hole coupling outer, Microcirculatory Observations platform 7 aligming microscope objective lens; Fixing head 2 is placed on above the thermal treatment zone 5, observes draw-in groove 11 holding in joint pin 15 outer.
Other structures are identical with embodiment 1 with operational approach.
Embodiment 2 fixing, 2 to be arranged on perfusion box 1 and outside microscope, for large-area fixing head 2 provides sufficient space, can to place volume comparatively big experiment animal.
embodiment 3
As shown in Figure 5, composition graphs 1-3, embodiment 3 as different from Example 1 embodiment 3 arranges the connectivity slot 17 of intercommunicating pore 19 alternate embodiment 1 between the thermal treatment zone 5 and observation area 4, concrete, select sieve plate, embodiment 3 can filter the impurity of observation area 4, prevents the impurity inflow thermal treatment zone 5 from affecting electrothermal tube 9 and normally works.
Claims (5)
1. a microscope Mesentery microcirculation observation carrying apparatus, is characterized in that, comprises perfusion box (1), fixing head (2) and temperature control equipment (3);
Described perfusion box (1) comprises observation area (4), the thermal treatment zone (5) and clear area (6), Microcirculatory Observations platform (7) is set in described observation area (4), described Microcirculatory Observations platform (7) higher than the base plate place plane of observation area (4) and printing opacity, arranges temperature-sensing probe (8) in described observation area (4); Arrange electrothermal tube (9) in the described thermal treatment zone (5), the described thermal treatment zone (5) are communicated with observation area (4);
The bolt (16) described fixing head (2) being arranged more than one screw (10) and coordinate with described screw, also arranges the observation draw-in groove (11) of holding in observation platform (4) outer;
Described temperature control equipment (3) is connected by wire with electrothermal tube (9) and temperature-sensing probe (8).
2. carrying apparatus according to claim 1, is characterized in that, arranges connectivity slot (17) or intercommunicating pore (19) between the thermal treatment zone (5) and observation area (4).
3. carrying apparatus according to claim 1, is characterized in that, described perfusion box (1) also comprises the fixed area (12) be arranged on outside it, and the outer of described fixed area (12) arranges the holddown groove (13) be connected with microscope carrier.
4. carrying apparatus according to claim 1, is characterized in that, the opposite edge that draw-in groove (11) observed by described fixing head (2) arranges the fixed card slot (14) mated with microscope handel.
5. carrying apparatus according to claim 1, is characterized in that, described Microcirculatory Observations platform (7) is fixedly connected with by the base plate of joint pin (15) with observation area (4), and described joint pin (15) is ground glass material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520538021.9U CN204814273U (en) | 2015-07-23 | 2015-07-23 | Microcirculation of microscope mesenterium is observed with carrying thing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520538021.9U CN204814273U (en) | 2015-07-23 | 2015-07-23 | Microcirculation of microscope mesenterium is observed with carrying thing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204814273U true CN204814273U (en) | 2015-12-02 |
Family
ID=54671923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520538021.9U Active CN204814273U (en) | 2015-07-23 | 2015-07-23 | Microcirculation of microscope mesenterium is observed with carrying thing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204814273U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112034609A (en) * | 2020-11-04 | 2020-12-04 | 成都泰盟软件有限公司 | Novel animal mesentery microcirculation observation device |
-
2015
- 2015-07-23 CN CN201520538021.9U patent/CN204814273U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112034609A (en) * | 2020-11-04 | 2020-12-04 | 成都泰盟软件有限公司 | Novel animal mesentery microcirculation observation device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20210261903A1 (en) | Cell culture incubators with integrated imaging systems | |
| RU2010134422A (en) | METHOD AND DEVICE FOR ANALYSIS OF PARTICLES IN A LIQUID SAMPLE | |
| WO2022216957A1 (en) | Automated incubator with robotic transport | |
| CN103642688B (en) | For monitoring and/or cultivating the equipment, system and method for microscopic objects | |
| CN108474022A (en) | To contain the device and method of the matrix volume imaging of three-dimensional nucleic acid | |
| Gualda et al. | Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy | |
| KR101721349B1 (en) | Sampling assembly, microscope module, and microscope apparatus | |
| CN103196719B (en) | Used in transmission electron microscope micro-nano particle sample carries net storage facility and sample preparation methods | |
| Sun et al. | Needle-compatible single fiber bundle image guide reflectance endoscope | |
| US20120140056A1 (en) | Sample imaging system and method for transmitting an image of cells or tissues located in a culturing space to data prcessing means | |
| US20150045259A1 (en) | High-throughput platform for in-vivo sub-cellular screens on vertebrate larvae | |
| Lin et al. | Autonomous system for cross-organ investigation of ethanol-induced acute response in behaving larval zebrafish | |
| CN100575930C (en) | The micro-fluorescence spectrum measuring apparatus of fluid inclusion | |
| CN204814273U (en) | Microcirculation of microscope mesenterium is observed with carrying thing device | |
| CN216082488U (en) | Fold line light path device capable of switching double irradiation channels through multi-power objective lens | |
| CN104921838A (en) | Constant temperature and humidity perfusion device for observation of mesentery microcirculation and application of constant temperature and humidity perfusion device | |
| CN204839817U (en) | Mesenterium microcirculation is observed with constant temperature and humidity perfusion device | |
| Pampaloni et al. | Light sheet-based fluorescence microscopy (LSFM) reduces phototoxic effects and provides new means for the modern life sciences | |
| Yuan et al. | A flexible chamber for time-lapse live-cell imaging with stimulated raman scattering microscopy | |
| KR20160037718A (en) | Vehicle for inspecting circulating tumor cells in blood | |
| Pampaloni et al. | A Small Guide on How to Mount a Sample in a Light‐Sheet Microscope | |
| DE202013100660U1 (en) | Device for oxygen measurement | |
| Paiè et al. | Dual-color on-chip light sheet microscopy of drosophila embryos | |
| US20240151691A1 (en) | Method and apparatus for illuminating and imaging organoids and spheroids | |
| US20230303959A1 (en) | Automated apparatus and method for in vitro fertilization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |