CN110037881A - A kind of stage apparatus for the observation of mouse cremaster - Google Patents

A kind of stage apparatus for the observation of mouse cremaster Download PDF

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Publication number
CN110037881A
CN110037881A CN201910405351.3A CN201910405351A CN110037881A CN 110037881 A CN110037881 A CN 110037881A CN 201910405351 A CN201910405351 A CN 201910405351A CN 110037881 A CN110037881 A CN 110037881A
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CN
China
Prior art keywords
observation
cremaster
chamber
support frame
fixed column
Prior art date
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Pending
Application number
CN201910405351.3A
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Chinese (zh)
Inventor
张艳达
王家美
梁春
丁茹
贺治青
张治宇
厉娜
陈慧
张欣栋
张刘洋
顾宁越
徐峰
伍锋
丛晓亮
赵健
吴宗贵
杨本钊
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Shanghai Changzheng Hospital
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Shanghai Changzheng Hospital
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Publication date
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Priority to CN201910405351.3A priority Critical patent/CN110037881A/en
Publication of CN110037881A publication Critical patent/CN110037881A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61GTRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
    • A61G13/00Operating tables; Auxiliary appliances therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61GTRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
    • A61G13/00Operating tables; Auxiliary appliances therefor
    • A61G13/10Parts, details or accessories

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Accommodation For Nursing Or Treatment Tables (AREA)

Abstract

The present invention relates to a kind of stage apparatus for the observation of mouse cremaster, front tooth fixed column is arranged in one end of support plate, other end setting observation chamber, multiple cremaster fixed columns are arranged in the surrounding for observing chamber, cremaster fixing line is fixed in cremaster fixed column, force snesor is set at least one cremaster fixed column, and force snesor connects cremaster fixing line, fixes front tooth fixing line in front tooth fixed column;Air inlet and air outlet are respectively set in the side of observation chamber, top is upper cover plate, upper cover plate is connect by hinge with observation chamber, support frame is respectively set in observation chamber up and down, the fixed optical mirror slip of support frame and heater, reserved chamber is set between support frame and support plate, observes the side setting inlet and liquid outlet of chamber.The present invention is applicable to a variety of microcirculation monitorings, is particularly suitable for a variety of demand integration of narrow space, facilitates observation, prevent observation space conflict, operating space insufficient.

Description

A kind of stage apparatus for the observation of mouse cremaster
Technical field
A variety of microcirculations are applicable to the present invention relates to medical experimental equipment technical field, especially one kind to monitor, especially It is suitable for a variety of demands of narrow space to integrate, facilitates observation, prevents observation space conflict, operating space insufficient for mouse The stage apparatus of cremaster observation.
Background technique
Cremaster: dissection name.One of be combined into outside cremaster system spermatic cord, master is made of fascicula.Muscle bundle borrows connective group Knit connection.Muscle bundle comes from oblique and musculus trasversus abdomins, with spermatic cord through annulus inguinalis subcutaneus, wraps spermatic cord downwards, testis and attached Testis.
Cremaster is the emphasis studied now as the intravital microcirculation watch window similar with skin of back, mesenterium One of, but existing cremaster Microcirculation observation platform is mostly that laboratory is built according to respective demand at present, and lacking one can It integrates a variety of physiologic signal monitorings while being able to satisfy the comprehensive platform of mechanics and a variety of optical observations, simultaneously as optical observation is empty Between it is narrow, how to integrate a variety of detection means on limited hardware platform, meet laboratory increasingly narrow space requirement It is one of current observation platform urgent problem to be solved.The platform of existing observation mouse cremaster is mostly that laboratory is respectively built, Distinct device different hardware mixing, lack a unified referential common platform, cause the relevant technologies develop and carry out compared with It is slow.A more comprehensive observation platform is needed, holds physiology monitoring, pharmaceutical intervention, mechanics and optical evaluation and is integrated, Help to realize the extensive development and research application of Mouse Cremaster Muscle Preparation For Microcirculatory assessment.
It needs one kind to be applicable to a variety of microcirculations monitoring, be particularly suitable for a variety of demands integration of narrow space, conveniently The stage apparatus observed, prevent observation space conflict, operating space insufficient for the observation of mouse cremaster.
Summary of the invention
It is applicable to that a variety of microcirculations monitoring, to be particularly suitable for narrow space a variety of the object of the present invention is to provide one kind Demand integration, the platform dress for facilitating observation, preventing observation space conflict, operating space insufficient for the observation of mouse cremaster It sets.
A kind of stage apparatus for the observation of mouse cremaster, comprising:
Front tooth fixed column is arranged in support plate, one end of the support plate, and other end setting observation chamber is described to observe the four of chamber Multiple cremaster fixed columns are set in week, fix cremaster fixing line in the cremaster fixed column, at least one cremaster is fixed Force snesor is set on column, and force snesor connects cremaster fixing line, fixes front tooth fixing line in the front tooth fixed column;
Air inlet and air outlet are respectively set in the side of the observation chamber, and top is upper cover plate, and the upper cover plate passes through hinge Chain is connect with observation chamber, and support frame, the fixed optical mirror slip of support frame as described above and heater, institute is respectively set in the observation chamber up and down It states and reserved chamber is set between support frame and support plate, the side setting inlet and liquid outlet of the observation chamber.
Light transmitting sheet is set between support frame as described above.
The front tooth fixed column and cremaster fixed column are respectively Nut column.
The observation chamber is closed barrel-shaped.
The air inlet and air outlet are between support frame.
Support frame as described above is ring-type, is vacantly observing intracavitary portion.
Sealing ring is set between the optical mirror slip and support frame.
The heater is located at the lower section of support frame.
The position of the inlet is higher than the position of liquid outlet.
Front tooth fixed column is arranged in one end of support plate of the present invention, and chamber is observed in other end setting, and the surrounding setting for observing chamber is more A cremaster fixed column fixes cremaster fixing line in cremaster fixed column, and power is arranged at least one cremaster fixed column and passes Sensor, force snesor connect cremaster fixing line, fix front tooth fixing line in front tooth fixed column;The side of observation chamber is respectively set Air inlet and air outlet, top are upper cover plate, and upper cover plate is connect by hinge with observation chamber, and support is respectively set in observation chamber up and down Frame, the fixed optical mirror slip of support frame and heater, are arranged reserved chamber between support frame and support plate, observe chamber side be arranged into Liquid mouth and liquid outlet.Integral platform combines together to be multi-functional, reduces different monitoring modulars and is additionally superimposed caused observation sky Between conflict and operating space it is insufficient the problems such as;The physiological functional moni-toring of observation animal can be realized simultaneously, exogenous drugs intervention, mentioned The information of a variety of observation signals such as testis muscular strength and microcirculation optics exports;By heating module cyclic annular under observation platform and The design of hot-air convection system, the interference of aqueous vapor when utmostly reducing optical observation microcirculating state, while being microcirculation Long-term observation corresponding isoperibol is provided, in conjunction with cremaster surface thin glass sheet realize with the part of external environment every From, interference of the ambient temperature for Microcirculatory Observations, while the integration of related liquid instillation drainage system are reduced, it can be utmostly It is provided for cremaster and meets the states such as physiological hydrodynamic lubrication and metabolin export.The present invention is applicable to a variety of microcirculation prisons It surveys, is particularly suitable for a variety of demand integration of narrow space, facilitates observation, prevent observation space conflict, operating space insufficient.
Detailed description of the invention
Fig. 1 is top view of the invention;
Fig. 2 is the cross-sectional view of present invention observation chamber;
In figure: 1, support plate, 2, front tooth fixed column, 3, front tooth fixing line, 4, cremaster fixed column, 5, cremaster fixes Line, 6, force snesor, 7, observation chamber, 8, air inlet, 9, gas outlet, 10, support frame, 11, optical mirror slip, 12, heater, 13, Reserved chamber, 14, hinge, 15, inlet, 16, liquid outlet, 17, upper cover plate, 18, light transmitting sheet.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
A kind of stage apparatus for the observation of mouse cremaster, comprising: one end setting front tooth of support plate 1, support plate 1 is solid Fixed column 2, the other end, which is arranged, observes chamber 7, the multiple cremaster fixed columns 4 of surrounding setting of observation chamber 7, solid in cremaster fixed column 4 Determine cremaster fixing line 5, force snesor 6 is set at least one cremaster fixed column 4, force snesor 6 connects cremaster and fixes Line 5 fixes front tooth fixing line 3 in front tooth fixed column 2;Air inlet 8 and gas outlet 9 is respectively set in the side of observation chamber 7, and top is Upper cover plate 17, upper cover plate 17 are connect by hinge 14 with observation chamber 7, and support frame 10, support frame 10 is respectively set in about 7 chamber of observation Reserved chamber 13, the side setting of observation chamber 7 is arranged in fixed optical mirror slip 11 and heater 12 between support frame 10 and support plate 1 Inlet 15 and liquid outlet 16.
Light transmitting sheet 18 is set between support frame 10.Front tooth fixed column 2 and cremaster fixed column 4 are respectively Nut column.Observation Chamber 7 is closed barrel-shaped.Air inlet 8 and gas outlet 9 are between support frame 10.Support frame 10 is ring-type, is vacantly being observed Inside chamber 7.Sealing ring is set between optical mirror slip 11 and support frame 10.Heater 12 is located at the lower section of support frame 10.Inlet 15 position is higher than the position of liquid outlet 16.
Multiple front tooth fixed columns 2, other end setting observation chamber 7, the surrounding setting of observation chamber 7 is arranged in one end of support plate 1 Multiple cremaster fixed columns 4 are fixed cremaster fixing line 5 in cremaster fixed column 4, are set at least one cremaster fixed column 4 Force snesor 6 is set, force snesor 6 connects cremaster fixing line 5.Experimental animal is fixed in support plate 1, cremaster fixed column 4 Cooperate with cremaster fixing line 5, the cremaster of experimental animal is fixed on inside observation chamber 7, force snesor 6 is for detecting fixation Power, and being adjusted fixes cremaster convenient for adjusting suitable pulling force.Front tooth fixed column 2 and front tooth fixing line 3 are for fixing Experimental animal prevents its movement, influences experiment process.
Air inlet 8 and gas outlet 9, the side setting inlet 15 of observation chamber 7 and out liquid is respectively set in the side of observation chamber 7 Mouth 16 simulates actual occasion, improves the accuracy of experiment to observation 7 air inlet of chamber and feed liquor.Top is upper cover plate 17, upper cover Plate 17 by hinge 14 with observe chamber 7 connect, facilitate and be put into cremaster, cremaster recloses after being put into, provide one it is closed Space, improve the constant temperature effect of experimental situation.Support frame 10, the fixed optical frames of support frame 10 is respectively set in about 7 chamber of observation Reserved chamber 13 is arranged in piece 11 and heater 12 between support frame 10 and support plate 1, facilitate upper and lower part place optical lens into Row observation, efficiently uses space.
Light transmitting sheet 18 is set between support frame 10, places cremaster on the support frame 10 of lower part, covers light transmitting sheet on it 18, observing effect is provided.Front tooth fixed column 2 and cremaster fixed column 4 are respectively Nut column, tightening force can be adjusted. Observe chamber 7 be it is closed barrel-shaped, facilitate the folding of upper cover plate 17.
Air inlet 8 and gas outlet 9 efficiently use the hot gas of entrance between support frame 10, improve constant temperature effect.Support Frame 10 is ring-type, vacantly inside observation chamber 7, shortens observation space, effectively improves space utilization rate.Optical mirror slip 11 and support Sealing ring is set between frame 10, prevents the gentle body outflow of water.Heater 12 is located at the lower section of support frame 10, facilitates and is heated, Improve heat transfer effect.The position of inlet 15 is higher than the position of liquid outlet 16, prevents liquid countercurrent.
Integral platform combines together to be multi-functional, reduces different monitoring modulars and is additionally superimposed caused observation space conflict The problems such as insufficient with operating space;Physiological functional moni-toring, the exogenous drugs intervention, cremaster of observation animal can be realized simultaneously The information export of a variety of observation signals such as power detection and cremaster Microcirculation observation;Pass through heating module cyclic annular under observation platform And the design of hot-air convection system, the interference of aqueous vapor when utmostly reducing optical observation microcirculating state, while being micro- The long-term observation of circulation provides corresponding isoperibol, realizes the part with external environment in conjunction with the thin glass sheet on cremaster surface Isolation reduces interference of the ambient temperature for Microcirculatory Observations, while the integration of related liquid instillation drainage system, can maximum journey Degree provides for cremaster meets the states such as physiological hydrodynamic lubrication and metabolin export.
Back part of animal contact portion, which can be directly printed on Supporting Media by circuit board, realizes a variety of physiological signal (hearts Electricity, breathing etc.) direct monitoring, reduce Legacy Status under connect interference of the various conducting wires to observation platform;Integral platform can connect With gas anesthesia, perhaps ventilator Intubaction device to realize mouse gas anesthesia or opens the prison of the microcirculation function under chest state It surveys;Cremaster lower support structure is reserved with the space of inverted microscope camera lens, by reducing the optical medium of whole optical path kind, with Realize optical imaging effect that is relatively sharp and less interfering;
Optical observation window is three layers of design for disassembly, and centre is the cremaster tissue being observed, and upper and lower level is replaceable High light transmittance thin slice, the optical medium of different-thickness difference optical characteristics can be selected as needed, it is each utmostly to meet The demand of kind different experiments.
Integral platform combines together to be multi-functional, reduces different monitoring modulars and is additionally superimposed caused observation space conflict The problems such as insufficient with operating space;Physiological functional moni-toring, the exogenous drugs intervention, cremaster power of observation animal can be realized simultaneously It learns and the information of a variety of observation signals such as microcirculation optics exports;Pass through heating module cyclic annular under observation platform and hot-air The design of contracurrent system, the interference of aqueous vapor when utmostly reducing optical observation microcirculating state, while being the long-term of microcirculation Observation provides corresponding isoperibol, is isolated in conjunction with the thin glass sheet realization on cremaster surface with the part of external environment, reduces Interference of the ambient temperature for Microcirculatory Observations, while the integration of related liquid instillation drainage system utmostly can mention testis Flesh offer meets the states such as physiological hydrodynamic lubrication and metabolin export;
Back part of animal contact portion, which can be directly printed on Supporting Media by circuit board, realizes a variety of physiological signal (hearts Electricity, breathing etc.) direct monitoring, reduce Legacy Status under connect interference of the various conducting wires to observation platform;Integral platform can connect With gas anesthesia, perhaps ventilator Intubaction device to realize mouse gas anesthesia or opens the prison of the microcirculation function under chest state It surveys;Cremaster lower support structure is reserved with the space of inverted microscope camera lens, by reducing the optical medium of whole optical path kind, with Realize optical imaging effect that is relatively sharp and less interfering;Optical observation window is three layers of design for disassembly, and centre is to be seen The cremaster tissue of survey, upper and lower level are replaceable high light transmittance thin slice, can select different-thickness difference optics special as needed The optical medium of property, utmostly to meet the needs of various different experiments.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe originals of the invention Reason, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these changes and improvements It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle It is fixed.

Claims (9)

1. a kind of stage apparatus for the observation of mouse cremaster characterized by comprising
Front tooth fixed column (2) are arranged in one end of support plate (1), the support plate (1), other end setting observation chamber (7), the sight Multiple cremaster fixed columns (4) are arranged in the surrounding for examining chamber (7), fix cremaster fixing line on the cremaster fixed column (4) (5), force snesor (6) being set at least one cremaster fixed column (4), force snesor (6) connects cremaster fixing line (5), Front tooth fixing line (3) are fixed on the front tooth fixed column (2);
Air inlet (8) and gas outlet (9) is respectively set in the side of observation chamber (7), and top is upper cover plate (17), the upper cover Plate (17) is connect by hinge (14) with observation chamber (7), and support frame (10), the branch is respectively set in the observation chamber (7) up and down Reserved chamber is arranged in support (10) fixed optical mirror slip (11) and heater (12) between support frame as described above (10) and support plate (1) (13), side setting inlet (15) of observation chamber (7) and liquid outlet (16).
2. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the support Light transmitting sheet (18) are set between frame (10).
3. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the front tooth Fixed column (2) and cremaster fixed column (4) are respectively Nut column.
4. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the observation Chamber (7) is closed barrel-shaped.
5. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the air inlet Mouth (8) and gas outlet (9) is located between support frame (10).
6. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the support Frame (10) is ring-type, vacantly internal in observation chamber (7).
7. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the optics Sealing ring is set between eyeglass (11) and support frame (10).
8. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the heating Device (12) is located at the lower section of support frame (10).
9. a kind of stage apparatus for the observation of mouse cremaster according to claim 1, which is characterized in that the feed liquor The position of mouth (15) is higher than the position of liquid outlet (16).
CN201910405351.3A 2019-05-16 2019-05-16 A kind of stage apparatus for the observation of mouse cremaster Pending CN110037881A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910405351.3A CN110037881A (en) 2019-05-16 2019-05-16 A kind of stage apparatus for the observation of mouse cremaster

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Application Number Priority Date Filing Date Title
CN201910405351.3A CN110037881A (en) 2019-05-16 2019-05-16 A kind of stage apparatus for the observation of mouse cremaster

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001133392A (en) * 1999-11-08 2001-05-18 Okabe Co Ltd Measuring method of fixing force for anchor material
JP2006275796A (en) * 2005-03-29 2006-10-12 National Institute Of Advanced Industrial & Technology Fastener and its use
WO2007036318A1 (en) * 2005-09-23 2007-04-05 Universitätsklinikum Freiburg Osteosynthesis aid
US20100145154A1 (en) * 2008-08-28 2010-06-10 Bayer Healthcare Llc Rodent Cremaster Preparation Device for Intravital Microscopy
KR20110001309U (en) * 2009-07-30 2011-02-09 한국화학융합시험연구원 Thermostatic device
CN104921838A (en) * 2015-07-23 2015-09-23 河北医科大学第一医院 Constant temperature and humidity perfusion device for observation of mesentery microcirculation and application of constant temperature and humidity perfusion device
CN108498204A (en) * 2018-05-09 2018-09-07 首都医科大学宣武医院 A kind of animal spinal cord living imaging Orientation observation device and its application method
CN210056629U (en) * 2019-05-16 2020-02-14 上海长征医院 Platform device for observing mouse cremaster muscle

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001133392A (en) * 1999-11-08 2001-05-18 Okabe Co Ltd Measuring method of fixing force for anchor material
JP2006275796A (en) * 2005-03-29 2006-10-12 National Institute Of Advanced Industrial & Technology Fastener and its use
WO2007036318A1 (en) * 2005-09-23 2007-04-05 Universitätsklinikum Freiburg Osteosynthesis aid
US20100145154A1 (en) * 2008-08-28 2010-06-10 Bayer Healthcare Llc Rodent Cremaster Preparation Device for Intravital Microscopy
KR20110001309U (en) * 2009-07-30 2011-02-09 한국화학융합시험연구원 Thermostatic device
CN104921838A (en) * 2015-07-23 2015-09-23 河北医科大学第一医院 Constant temperature and humidity perfusion device for observation of mesentery microcirculation and application of constant temperature and humidity perfusion device
CN108498204A (en) * 2018-05-09 2018-09-07 首都医科大学宣武医院 A kind of animal spinal cord living imaging Orientation observation device and its application method
CN210056629U (en) * 2019-05-16 2020-02-14 上海长征医院 Platform device for observing mouse cremaster muscle

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