CN113058672B - Detachable pipettor with filtering device and manufacturing method thereof - Google Patents
Detachable pipettor with filtering device and manufacturing method thereof Download PDFInfo
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- CN113058672B CN113058672B CN202110404763.2A CN202110404763A CN113058672B CN 113058672 B CN113058672 B CN 113058672B CN 202110404763 A CN202110404763 A CN 202110404763A CN 113058672 B CN113058672 B CN 113058672B
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Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
- B01D35/02—Filters adapted for location in special places, e.g. pipe-lines, pumps, stop-cocks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
- B01D46/0027—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions
- B01D46/0028—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions provided with antibacterial or antifungal means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
- B01D46/54—Particle separators, e.g. dust precipitators, using ultra-fine filter sheets or diaphragms
- B01D46/543—Particle separators, e.g. dust precipitators, using ultra-fine filter sheets or diaphragms using membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/26—Drying gases or vapours
- B01D53/28—Selection of materials for use as drying agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/025—Reverse osmosis; Hyperfiltration
Abstract
The invention discloses a detachable liquid transfer device with a filtering device, which comprises a liquid transfer device main body, a connecting piece, the filtering device and a liquid suction nozzle, wherein the liquid transfer device main body is connected with the liquid suction nozzle through the connecting piece; meanwhile, a manufacturing method of the detachable pipettor with the filtering device is also disclosed. According to the invention, through the improvement of the filter element and the pipette structure, the pollution to the tail end of the connecting part of the pipette main body and the pipette nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the component by a user and the possible damage to the whole pipettor caused by the cleaning process are avoided.
Description
Technical Field
The invention relates to a detachable pipettor with a filtering device and a manufacturing method thereof, belonging to the technical field of biological, chemical and medical instruments.
Background
In the fields of biology, chemistry and medicine, a liquid transfer device is a common tool for quantitatively sucking various liquids, and when the liquid is sucked and discharged, if the action is fast, and the liquid surface tension is added, small liquid bubbles may be formed at the upper end of a liquid suction nozzle, which is close to the tail end of a connecting part of a liquid transfer device main body and the liquid suction nozzle. After the small bubbles are broken, the end of the connection part between the pipette body and the pipette nozzle may be contaminated. In this case, even if the plastic pipette tip is frequently replaced, contamination of the subsequent pipetting operation is likely to occur.
After the sample is added, the button is detached according to the liquid suction nozzle, and the liquid suction nozzle is discarded. If the release button is released too early, the residual liquid in the pipette tip may be sucked back to the end of the connection member between the pipette body and the pipette tip. Particularly, in the experiments related to molecular biology, even if only a very small amount of DNA sample aerosol is adsorbed at the end of the connecting part between the pipette main body and the pipette nozzle due to the above-mentioned back-suction effect, the experiment result may be significantly deviated due to the strong amplification effect of PCR during the sample application operation of PCR.
The existing filtering device is only one layer or two layers of filter membranes which are made of a single artificial polymeric material, when the existing filtering device faces different solution or suspension samples encountered in use due to physicochemical characteristics and thickness, the artificial polymeric material from a single raw material is difficult to adapt to the requirements of the samples with different properties and biological characteristics, and the aspects of antibiosis, biological pollution resistance and the like can not completely meet the requirements of accurate liquid transfer in molecular biology and medical science.
Due to the pollution of various organic and inorganic particles and aerosol in the air, the liquid transfer operation is an important part of molecular biology and medical science laboratory operation which needs to be repeated and carried out for a long time, and particularly in gene information research experiments relating to human beings or other organisms, tiny biological pollution of liquid transfer samples can cause great probability of test result errors. The single filter element structure, although achieving the purpose of replacing and avoiding serious microbial contamination, does not achieve the purpose of preventing DNA, RNA and protein and micro-particle contamination in biological specimens completely.
Disclosure of Invention
The invention aims to provide a detachable pipettor with a filtering device and a manufacturing method of the detachable pipettor with the filtering device, and the invention fundamentally avoids the pollution to the tail end of a connecting part of a pipettor main body and a liquid suction nozzle and the influence on an experimental result by improving the structure of a filter element and the pipettor; the untimely and nonstandard cleaning of the pipette by a user and the possible damage to the whole pipette caused by the cleaning process are avoided.
In order to solve the technical problems, the invention adopts the following technical scheme: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body, a connecting piece, the filtering device and a liquid suction nozzle, wherein the liquid transfer device main body is connected with the liquid suction nozzle through the connecting piece; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the component by a user and the possible damage to the whole pipettor caused by the cleaning process are avoided.
The connector comprises an outer sleeve and a pipette, the outer sleeve is sleeved on the outer surface of the pipette, the tail end of the pipette protrudes out of the tail end of the outer sleeve, a dustproof cover is arranged on the tail end of the outer sleeve, and one end of the pipette protruding out of the tail end of the outer sleeve is arranged in the dustproof cover; in a biological laboratory or other location, the dust cap prevents contamination of the tip of the pipette from airborne particulates and aerosols when the pipette is temporarily not in use for a short period of time.
The detachable pipette with the filtering device comprises a pipe section A, a pipe section B and a pipe section C, wherein the tail end of the pipe section A is detachably connected with the top end of the pipe section B, the inner diameter of the tail end of the pipe section A is larger than the outer diameter of the top end of the pipe section B, the pipe section C is arranged at the tail end of the pipe section B and is connected with a pipette nozzle, a limiting ring is arranged on the inner surface of the tail end of the pipe section A, an A sealing ring is arranged between the top end of the pipe section B and the limiting ring, the diameter of the A sealing ring is the same as that of the limiting ring, the inner diameter of the A sealing ring is the same as that of the top end of the pipe section B, the filtering device is arranged on the inner surface of the tail end of the pipe section B, the inner surface of the pipette nozzle is detachably connected with the outer surface of the pipe section C, the pipette has detachable and replaceable functions, when the pipette is used for a long time and the pipe section B and the pipe section C are seriously polluted, the replacement of the entire pipette is avoided, use cost is reduced, and the A sealing washer is arranged between A pipeline section and B pipeline section, makes the connection of A pipeline section and B pipeline section inseparabler, prevents the liquid seepage, and the spacing ring provides supporting platform for the A sealing washer.
According to the detachable pipette with the filtering device, the inner surface of the tail end of the pipe section A is provided with the internal thread, the outer surface of the top end of the pipe section B is provided with the external thread corresponding to the internal thread, and the internal thread is in threaded connection with the external thread.
According to the detachable pipette with the filtering device, the inner surface of the tail end of the pipe section A is provided with the plurality of grooves, the outer surface of the top end of the pipe section B is provided with the convex rings corresponding to the grooves, and the grooves are clamped with the convex rings.
According to the detachable pipettor with the filtering device, the inner surface of the tail end of the pipe section A is provided with the magnet ring A, the top end of the pipe section B is provided with the magnet ring B, the magnet ring A is connected with the magnet ring B in an adsorption mode, the diameter of the magnet ring A is the same as that of the limiting ring, the outer diameter of the magnet ring B is smaller than that of the magnet ring A, the inner diameter of the magnet ring B is the same as that of the magnet ring A, the outer surface of the top end of the pipe section B is provided with the sealing ring B, and the sealing ring B is arranged between the inner surface of the tail end of the pipe section A and the outer surface of the top end of the pipe section B; the sealing ring B tightly connects the inner surface of the tail end of the pipe section A with the outer surface of the top end of the pipe section B, so that liquid leakage is prevented.
The filter device comprises a transparent polymer shell, the transparent polymer shell is of a hollow structure and is made of polypropylene or polyether sulfone materials, a polymer filter membrane layer, a sterilization membrane layer, a granular desiccant layer, an indication layer and a reverse osmosis layer are sequentially arranged in an inner cavity of the transparent polymer shell from bottom to top, and the polymer filter membrane layer, the sterilization membrane layer, the granular desiccant layer, the indication layer and the reverse osmosis layer are equidistantly arranged in the inner cavity of the transparent polymer shell at intervals; the pollution of biological origin at the tail end of the suction tube and the influence on the experimental result caused by single structure and imperfect function of the filter element are avoided fundamentally; meanwhile, because the inner cavity of the filtering device is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is realized, so that a user can be reminded of replacing the pipette and the filtering device in time, the situation that the filtering device is invalid and continues to be used is avoided, and the waste is avoided; the filtering device has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization and bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding various known pollutions to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the part after using the filtering device for a long time, and the possible damage and corrosion to the whole pipettor, in a molecular biology experiment, like simultaneously operating a plurality of biological gene extract samples which are possible to be polluted mutually, the pipettor can be replaced for a plurality of times in the operation process, thereby completely avoiding cross pollution, avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, ensuring that the pipetting operation is more accurate and controllable, ensuring that the experimental result, particularly the quantitative PCR experimental result related to genome research, is more accurate and reliable, and the texture of the transparent polymer shell is hard and transparent, the color of the indicating layer is convenient to change, and the purpose of observation is achieved.
In the above detachable pipette with a filter device, the polymer filter membrane layer is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyethersulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilizing film layer comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular drying agent layer comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indicating layer comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer comprises an acetate fiber membrane or an aromatic polyamide (hydrazide) membrane; the polymer filter membrane layer is stacked in multiple layers by adopting non-toxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer achieves the aim of efficiently filtering organic pollutants such as micro dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; silver ions are coated on the sterilization film layer, active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfhydryl on bacterial cell membranes; the active silver ions interfere an electronic transmission system of bacterial respiration to kill bacteria, and the sterilization film layer achieves the aim of killing bacteria leaked from the first filtering film layer; the granular drying agent layer achieves the aim of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the filtering device of the pipettor to the maximum extent, and prolongs the service life of the pipettor; once water enters the indicating layer, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette with the filtering device immediately, and the user is prevented from judging whether to replace the pipette with the filtering device by personal assumption; the reverse osmosis layer achieves the purpose of isolating heavy metal ions, colloid, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and environment.
In the detachable pipette with the filtering device, the pore diameters of the polyvinylidene fluoride membrane, the polyethylene terephthalate membrane, the polyether sulfone modified microporous membrane, the polytetrafluoroethylene membrane, the mixed cellulose ester membrane or the polycarbonate microporous membrane are all 0.4-0.45 μm, the pore diameter of the polypropylene membrane is 40-50 μm, the pore diameter of the borosilicate glass fiber efficient filter membrane is 0.22-0.45 μm, the diameter of the cobalt-free allochroic silica gel dry particles is 0.5-1mm, the thickness of the granular drying agent layer is 3-5mm, the thickness of the indicating layer is 2-3mm, and the pore diameter of the acetate fiber membrane or the aromatic polyamide (hydrazide) membrane is 0.5-10 nm.
A manufacturing method of a detachable pipettor with a filtering device comprises the following steps:
s1, manufacturing a filtering device in a closed environment with the temperature of 10-25 ℃ and the humidity of 50-75% under atmospheric pressure, cutting any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane with the membrane aperture of 0.22-0.45 mu m to a wafer with the inner diameter of a transparent polymer shell, stacking to manufacture a polymer filter membrane layer, wherein the polymer filter membrane layer is fixedly arranged at a position 1-2mm away from a bottom opening of the transparent polymer shell; then taking a polyethylene terephthalate film, cutting the polyethylene terephthalate film into a circular sheet with the inner diameter of the transparent polymer shell, coating silver ions on two surfaces of the polyethylene terephthalate film circular sheet to manufacture a sterilization film layer, and fixedly installing the sterilization film layer above the polymer filter film layer; then taking four polypropylene films, cutting the four polypropylene films into a circular sheet with the inner diameter of the transparent polymer shell, fixedly installing the first polypropylene film above the sterilization film layer, paving cobalt-free allochroic silica gel dry particles with the size of 0.5-1mm on the first polypropylene film, leveling, paving the cobalt-free allochroic silica gel dry particles with the thickness of 3-5mm, fixedly installing the second polypropylene film on the leveled cobalt-free allochroic silica gel dry particles, and manufacturing into a granular drying agent layer; fixedly installing a third polypropylene film above the granular desiccant layer, paving anhydrous copper sulfate powder with the thickness of 1-2mm on the third polypropylene film, leveling, and fixedly installing a fourth polypropylene film on the leveled anhydrous copper sulfate powder to manufacture an indication layer; taking an acetate fiber membrane or an aromatic polyamide (hydrazide) membrane, cutting the acetate fiber membrane or the aromatic polyamide (hydrazide) membrane into a round sheet with the inner diameter of the transparent polymer shell, manufacturing a reverse osmosis layer, and fixedly installing the reverse osmosis layer above the indication layer;
s2, sterilizing the manufactured filter device under the irradiation of gamma rays;
s3, installing the filter device which is sterilized on the inner surface of the tail end of the pipe section B;
s4, connecting the pipe section B provided with the filtering device with the pipe section A to form a pipette;
s5, connecting the pipette with the pipette main body;
and S6, sleeving the outer sleeve on the outer surface of the pipette and connecting the outer sleeve with the pipette main body.
Compared with the prior art, the liquid suction device comprises a liquid suction device main body, a connecting piece, a filtering device and a liquid suction nozzle, wherein the liquid suction nozzle main body is connected with the liquid suction nozzle through the connecting piece, the filtering device is arranged in an inner cavity of the connecting piece, the filtering device is arranged at a position close to the connecting piece and the liquid suction nozzle, a liquid suction nozzle dismounting button is arranged on the liquid suction nozzle main body, the connecting piece comprises a plurality of sections of pipe sections which are detachably connected, the filtering device is a transparent composite biological safety filter element device, the filtering device comprises a transparent polymer shell, and a polymer filter membrane layer, a sterilization membrane layer, a granular drying agent layer, an indicating layer and a reverse osmosis layer are sequentially arranged in the inner cavity of the transparent polymer shell from bottom to top, the liquid suction tube has detachable and replaceable functions, when the liquid suction device is used for a long time, and a B pipe section and a C pipe section are seriously polluted, the replacement of the whole liquid suction device is avoided, the use cost is reduced, the polymer filter membrane layer in the transparent polymer shell is stacked in a multi-layer mode by adopting non-toxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer achieves the purpose of efficiently filtering organic pollutants such as micro-dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; silver ions are coated on the bactericidal membrane layer, and active silver ions Ag2+ have high oxidation-reduction potential and react with sulfydryl on the bacterial cell membrane to destroy the biochemical reaction of bacterial protein; the active silver ions interfere an electronic transmission system of bacterial respiration to kill bacteria, and the sterilization film layer achieves the aim of killing bacteria leaked from the first filtering film layer; the granular drying agent layer achieves the aim of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the filtering device of the pipettor to the maximum extent, and prolongs the service life of the pipettor; once water enters the indicating layer, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette with the filtering device immediately, and the user is prevented from judging whether to replace the pipette with the filtering device by personal assumption; the reverse osmosis layer achieves the purpose of isolating heavy metal ions, colloid, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and the environment as a last barrier, so that the pollution to the tail end of the connecting part of the liquid suction nozzle and the main body of the liquid transfer device and the influence on an experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the component by a user and the possible damage to the whole pipettor caused by the cleaning process are avoided.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view showing the construction of an outer sleeve, a pipette and a dust cap in the present invention;
FIG. 3 is a schematic structural view of a stop collar, an A-ring, internal threads and external threads of the present invention;
FIG. 4 is a schematic structural view of a stop collar, a seal ring A, a groove and a convex ring of the present invention;
FIG. 5 is a schematic structural view of a stop collar, a seal ring A, a magnet ring B and a seal ring B of the present invention;
fig. 6 is a schematic view of the structure of the filter device of the present invention.
Reference numerals are as follows: 1-pipette body, 2-connecting piece, 3-filtering device, 4-pipette tip, 5-pipette tip discharge button, 6-outer sleeve, 7-pipette tip, 8-A tube section, 9-B tube section, 10-C tube section, 11-spacing ring, 12-A sealing ring, 13-internal thread, 14-external thread, 15-groove, 16-convex ring, 17-A magnet ring, 18-B magnet ring, 19-B sealing ring, 20-transparent polymer shell, 21-polymer filtering film layer, 22-sterilization film layer, 23-granular desiccant layer, 24-indication layer, 25-reverse osmosis layer and 26-dustproof cover.
The invention is further described with reference to the following figures and detailed description.
Detailed Description
Example 1 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on an experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the component by a user and the possible damage to the whole pipettor caused by the cleaning process are avoided.
Example 2 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on an experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents contamination of the tip of the pipette 7 by airborne fine particles and aerosols when the pipette is temporarily not in use for a short period of time.
Example 3 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, the filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, avoided the replacement to whole pipettor, reduced use cost, A sealing washer 12 is arranged between A pipeline section 8 and B pipeline section 9, makes the connection of A pipeline section 8 and B pipeline section 9 inseparabler, prevents the liquid seepage, and spacing ring 11 provides supporting platform for A sealing washer 12.
Example 4 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipette is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with an internal thread 13, the outer surface of the top end of the pipe section B9 is provided with an external thread 14 corresponding to the internal thread 13, and the internal thread 13 is in threaded connection with the external thread 14.
Example 5 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with a plurality of grooves 15, the outer surface of the top end of the pipe section B9 is provided with convex rings 16 corresponding to the grooves 15, and the grooves 15 are clamped with the convex rings 16.
Example 6 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with a magnet ring A17, the top end of the pipe section B9 is provided with a magnet ring B18, the magnet ring A17 is connected with the magnet ring B18 in an adsorption mode, the diameter of the magnet ring A17 is the same as that of the limiting ring 11, the outer diameter of the magnet ring B18 is smaller than that of the magnet ring A17, the inner diameter of the magnet ring B18 is the same as that of the magnet ring A17, the outer surface of the top end of the pipe section B9 is provided with a seal ring B19, and the seal ring B19 is arranged between the inner surface of the tail end of the pipe section A8 and the outer surface of the top end of the pipe section B9; the B sealing ring 19 tightly connects the inner surface of the tail end of the A pipe section 8 with the outer surface of the top end of the B pipe section 9, and liquid leakage is prevented.
Example 7 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filtering film layer 21, a sterilization film layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filtering film layer 21, the sterilization film layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are equidistantly arranged in the inner cavity of the transparent polymer shell 20 at intervals; the pollution of biological origin at the tail end of the suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are avoided fundamentally; meanwhile, because the inner cavity of the filtering device 3 is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is achieved, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the situation that the filtering device 3 is invalid and continues to be used is avoided, and meanwhile, waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization and bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding various known pollutions to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using for a long time, and the possible damage and corrosion to the whole pipettor, in a molecular biology experiment, like simultaneously operating a plurality of biological gene extract samples which are possible to be polluted mutually, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross pollution, avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, ensuring that the pipetting operation is more accurate and controllable, ensuring that the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the texture of the transparent polymer shell 20 is hard and transparent, the indicator layer 24 is readily colored for viewing purposes.
Example 8 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has detachable and replaceable functions, when the pipette tip is used for a long time and the B pipe section 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipette is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filtering film layer 21, a sterilization film layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filtering film layer 21, the sterilization film layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are equidistantly arranged in the inner cavity of the transparent polymer shell 20 at intervals; the biological pollution to the tail end of the liquid suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are fundamentally avoided; meanwhile, because the inner cavity of the filtering device 3 is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is achieved, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the situation that the filtering device 3 is invalid and continues to be used is avoided, and meanwhile, waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization and bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding various known pollutions to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using for a long time, and the possible damage and corrosion to the whole pipettor, in a molecular biology experiment, like simultaneously operating a plurality of biological gene extract samples which are possible to be polluted mutually, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross pollution, avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, ensuring that the pipetting operation is more accurate and controllable, ensuring that the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the texture of the transparent polymer shell 20 is hard and transparent, the color of the indicating layer 24 is convenient to change, so that the purpose of observation is achieved; the polymer filter membrane layer 21 is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer 22 comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular desiccant layer 23 comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indication layer 24 comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer 25 includes an acetate fiber film or an aromatic polyamide (hydrazide) film; the polymer filter membrane layer 21 is formed by stacking a plurality of nontoxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer 21 achieves the aim of efficiently filtering organic pollutants such as micro-dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; the bactericidal membrane layer 22 is coated with silver ions, the active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfydryl on the bacterial cell membrane; the active silver ions interfere the electronic transmission system of the bacteria breathing to kill the bacteria, and the sterilization film layer 22 achieves the purpose of killing the bacteria leaking from the first filtration film layer; the granular drying agent layer 23 achieves the purpose of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the pipette filtering device 3 to the maximum extent, and prolongs the service life of the pipette 7 of the pipette; once moisture enters the indicating layer 24, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette 7 with the filtering device 3 immediately, and the user is prevented from judging whether to replace the pipette 7 with the filtering device 3 by personal assumption; the reverse osmosis layer 25 serves the purpose of isolating heavy metal ions, colloids, viruses and biological macromolecules, and serves as the last barrier to isolate all proteins, RNA and DNA in the liquid and the environment.
Example 9 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, the filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a liquid suction pipe 7, the outer sleeve 6 is sleeved on the outer surface of the liquid suction pipe 7, the tail end of the liquid suction pipe 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the liquid suction pipe 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents the tip of the pipette 7 from being contaminated by fine particles and aerosols in the air when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filter membrane layer 21, a sterilization membrane layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filter membrane layer 21, the sterilization membrane layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are arranged in the inner cavity of the transparent polymer shell 20 at equal intervals; the biological pollution to the tail end of the liquid suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are fundamentally avoided; meanwhile, because the inner cavity of the filtering device 3 is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is achieved, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the situation that the filtering device 3 is invalid and continues to be used is avoided, and meanwhile, waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization, bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding all known pollution to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using the filtering device for a long time, and possibly causing damage and corrosion to the whole pipettor, in a molecular biology experiment, as simultaneously operating a plurality of possibly mutually polluted biological gene extract samples, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross contamination, and avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, so that the pipetting operation is more accurate and controllable, the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the transparent polymer shell 20 is hard and transparent in texture, the color of the indicating layer 24 is convenient to change, so that the purpose of observation is achieved; the polymer filter membrane layer 21 is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer 22 comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular desiccant layer 23 comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indication layer 24 comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer 25 includes an acetate fiber film or an aromatic polyamide (hydrazide) film; the polymer filter membrane layer 21 is formed by stacking a plurality of nontoxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer 21 achieves the aim of efficiently filtering organic pollutants such as micro-dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; the bactericidal membrane layer 22 is coated with silver ions, the active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfydryl on the bacterial cell membrane; the bacteria are killed by the electronic transmission system of the active silver ion interference bacteria respiration, and the sterilization film layer 22 achieves the purpose of killing the bacteria leaked from the first filtering film layer; the granular drying agent layer 23 achieves the purpose of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the pipette filtering device 3 to the maximum extent, and prolongs the service life of the pipette 7 of the pipette; once moisture enters the indicating layer 24, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette 7 with the filtering device 3 immediately, and the user is prevented from judging whether to replace the pipette 7 with the filtering device 3 by personal assumption; the reverse osmosis layer 25 achieves the purpose of isolating heavy metal ions, colloids, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and environment as a last barrier; the pore diameters of the polyvinylidene fluoride membrane, the polyethylene glycol terephthalate membrane, the polyether sulfone modified microporous membrane, the polytetrafluoroethylene membrane, the mixed cellulose ester membrane or the polycarbonate microporous membrane are all 0.4-0.45 mu m, the pore diameter of the polypropylene membrane is 40-50 mu m, the pore diameter of the borosilicate glass fiber high-efficiency filter membrane is 0.22-0.45 mu m, the diameter of the cobalt-free allochroic silica gel dry particles is 0.5-1mm, the thickness of the granular drying agent layer 23 is 3-5mm, the thickness of the indicating layer 24 is 2-3mm, and the pore diameter of the acetate fiber membrane or the aromatic polyamide (hydrazide) membrane is 0.5-10 nm.
Example 10 of the present invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents contamination of the tip of the pipette 7 by airborne fine particles and aerosols when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with an internal thread 13, the outer surface of the top end of the pipe section B9 is provided with an external thread 14 corresponding to the internal thread 13, and the internal thread 13 is in threaded connection with the external thread 14; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filtering film layer 21, a sterilization film layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filtering film layer 21, the sterilization film layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are equidistantly arranged in the inner cavity of the transparent polymer shell 20 at intervals; the biological pollution to the tail end of the liquid suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are fundamentally avoided; meanwhile, because the inner cavity of the filtering device 3 is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is achieved, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the situation that the filtering device 3 is invalid and continues to be used is avoided, and meanwhile, waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization, bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding all known pollution to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using the filtering device for a long time, and possibly causing damage and corrosion to the whole pipettor, in a molecular biology experiment, as simultaneously operating a plurality of possibly mutually polluted biological gene extract samples, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross contamination, and avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, so that the pipetting operation is more accurate and controllable, the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the transparent polymer shell 20 is hard and transparent in texture, the color of the indicating layer 24 is convenient to change, so that the purpose of observation is achieved; the polymer filter membrane layer 21 is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer 22 comprises a polyethylene terephthalate film coated with silver ions; the granular desiccant layer 23 comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indication layer 24 comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer 25 comprises a cellulose acetate membrane or an aromatic polyamide (hydrazide) membrane; the polymer filter membrane layer 21 is formed by stacking a plurality of nontoxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer 21 achieves the aim of efficiently filtering micro dust, aerosol and organic pollutants of microorganisms, mainly bacteria and large particles in air; the bactericidal membrane layer 22 is coated with silver ions, the active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfydryl on the bacterial cell membrane; the bacteria are killed by the electronic transmission system of the active silver ion interference bacteria respiration, and the sterilization film layer 22 achieves the purpose of killing the bacteria leaked from the first filtering film layer; the granular drying agent layer 23 achieves the purpose of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the pipette filtering device 3 to the maximum extent, and prolongs the service life of the pipette 7 of the pipette; once the indication layer 24 has moisture entering into the layer, anhydrous copper sulfate hydration reaction is immediately carried out to generate blue copper sulfate pentahydrate, so that a user is indicated to immediately replace the pipette 7 with the filtering device 3, and the situation that the user judges whether to replace the pipette 7 with the filtering device 3 or not by personal assumption is avoided; the reverse osmosis layer 25 achieves the purpose of isolating heavy metal ions, colloids, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and environment as a last barrier; the pore diameters of the polyvinylidene fluoride membrane, the polyethylene glycol terephthalate membrane, the polyether sulfone modified microporous membrane, the polytetrafluoroethylene membrane, the mixed cellulose ester membrane or the polycarbonate microporous membrane are all 0.4-0.45 mu m, the pore diameter of the polypropylene membrane is 40-50 mu m, the pore diameter of the borosilicate glass fiber high-efficiency filter membrane is 0.22-0.45 mu m, the diameter of the cobalt-free allochroic silica gel dry particles is 0.5-1mm, the thickness of the granular drying agent layer 23 is 3-5mm, the thickness of the indicating layer 24 is 2-3mm, and the pore diameter of the acetate fiber membrane or the aromatic polyamide (hydrazide) membrane is 0.5-10 nm.
Example 11 of the invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a pipette 7, the outer sleeve 6 is sleeved on the outer surface of the pipette 7, the tail end of the pipette 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the pipette 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents contamination of the tip of the pipette 7 by airborne fine particles and aerosols when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with a plurality of grooves 15, the outer surface of the top end of the pipe section B9 is provided with a convex ring 16 corresponding to the grooves 15, and the grooves 15 are clamped with the convex ring 16; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filter membrane layer 21, a sterilization membrane layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filter membrane layer 21, the sterilization membrane layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are arranged in the inner cavity of the transparent polymer shell 20 at equal intervals; the biological pollution to the tail end of the liquid suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are fundamentally avoided; meanwhile, because the inner cavity of the filtering device 3 is provided with the multilayer and multiple-effect transparent composite biological safety filter element, the color-changing indicating function is achieved, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the situation that the filtering device 3 is invalid and continues to be used is avoided, and meanwhile, waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization, bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding all known pollution to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using the filtering device for a long time, and possibly causing damage and corrosion to the whole pipettor, in a molecular biology experiment, as simultaneously operating a plurality of possibly mutually polluted biological gene extract samples, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross contamination, and avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, so that the pipetting operation is more accurate and controllable, the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the transparent polymer shell 20 is hard and transparent in texture, the color of the indicating layer 24 is convenient to change, so that the purpose of observation is achieved; the polymer filter membrane layer 21 is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer 22 comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular desiccant layer 23 comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indication layer 24 comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer 25 includes an acetate fiber film or an aromatic polyamide (hydrazide) film; the polymer filter membrane layer 21 is formed by stacking a plurality of nontoxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer 21 achieves the aim of efficiently filtering organic pollutants such as micro-dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; the bactericidal membrane layer 22 is coated with silver ions, the active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfydryl on the bacterial cell membrane; the bacteria are killed by the electronic transmission system of the active silver ion interference bacteria respiration, and the sterilization film layer 22 achieves the purpose of killing the bacteria leaked from the first filtering film layer; the granular drying agent layer 23 achieves the purpose of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the pipette filtering device 3 to the maximum extent, and prolongs the service life of the pipette 7 of the pipette; once moisture enters the indicating layer 24, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette 7 with the filtering device 3 immediately, and the user is prevented from judging whether to replace the pipette 7 with the filtering device 3 by personal assumption; the reverse osmosis layer 25 achieves the purpose of isolating heavy metal ions, colloids, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and environment as a last barrier; the pore diameters of the polyvinylidene fluoride membrane, the polyethylene glycol terephthalate membrane, the polyether sulfone modified microporous membrane, the polytetrafluoroethylene membrane, the mixed cellulose ester membrane or the polycarbonate microporous membrane are all 0.4-0.45 mu m, the pore diameter of the polypropylene membrane is 40-50 mu m, the pore diameter of the borosilicate glass fiber high-efficiency filter membrane is 0.22-0.45 mu m, the diameter of the cobalt-free allochroic silica gel dry particles is 0.5-1mm, the thickness of the granular drying agent layer 23 is 3-5mm, the thickness of the indicating layer 24 is 2-3mm, and the pore diameter of the acetate fiber membrane or the aromatic polyamide (hydrazide) membrane is 0.5-10 nm.
Example 12 of the present invention: a detachable liquid transfer device with a filtering device comprises a liquid transfer device main body 1, a connecting piece 2, a filtering device 3 and a liquid suction nozzle 4, wherein the liquid transfer device main body 1 is connected with the liquid suction nozzle 4 through the connecting piece 2, the filtering device 3 is arranged in an inner cavity of the connecting piece 2, the filtering device 3 is arranged at a position close to the connecting piece 2 and the liquid suction nozzle 4, a liquid suction nozzle dismounting button 5 is arranged on the liquid transfer device main body 1, the connecting piece 2 comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device 3 is a transparent composite biological safety filter element device; the pollution to the tail end of the connecting part of the liquid transfer device main body and the liquid suction nozzle and the influence on the experimental result are fundamentally avoided; the untimely and nonstandard cleaning of the part by a user and the possible damage to the whole pipettor in the cleaning process are avoided; the connecting piece 2 comprises an outer sleeve 6 and a liquid suction pipe 7, the outer sleeve 6 is sleeved on the outer surface of the liquid suction pipe 7, the tail end of the liquid suction pipe 7 protrudes out of the tail end of the outer sleeve 6, a dustproof cover 26 is arranged on the tail end of the outer sleeve 6, and one end, protruding out of the tail end of the outer sleeve 6, of the liquid suction pipe 7 is arranged in the dustproof cover 26; in a biological laboratory or other location, the dust cap 26 prevents contamination of the tip of the pipette 7 by airborne fine particles and aerosols when the pipette is temporarily not in use for a short period of time; the pipette 7 comprises an A pipe section 8, a B pipe section 9 and a C pipe section 10, wherein the tail end of the A pipe section 8 is detachably connected with the top end of the B pipe section 9, the inner diameter of the tail end of the A pipe section 8 is larger than the outer diameter of the top end of the B pipe section 9, the C pipe section 10 is arranged at the tail end of the B pipe section 9 and is connected with the pipette tip 4, a limiting ring 11 is arranged on the inner surface of the tail end of the A pipe section 8, an A sealing ring 12 is arranged between the top end of the B pipe section 9 and the limiting ring 11, the diameter of the A sealing ring 12 is the same as that of the limiting ring 11, the inner diameter of the A sealing ring 12 is the same as that of the top end of the B pipe section 9, the filtering device 3 is arranged on the inner surface of the tail end of the B pipe section 9, the inner surface of the pipette tip 4 is detachably connected with the outer surface of the C pipe section 10, and the pipette 7 has the detachable and replaceable functions, when the pipette tip 9 and the C pipe section 10 are seriously polluted, the replacement of the whole pipettor is avoided, the use cost is reduced, the A sealing ring 12 is arranged between the A pipe section 8 and the B pipe section 9, so that the connection between the A pipe section 8 and the B pipe section 9 is tighter, the liquid leakage is prevented, and the limiting ring 11 provides a supporting platform for the A sealing ring 12; the inner surface of the tail end of the pipe section A8 is provided with a magnet ring A17, the top end of the pipe section B9 is provided with a magnet ring B18, the magnet ring A17 is connected with the magnet ring B18 in an adsorption mode, the diameter of the magnet ring A17 is the same as that of the limiting ring 11, the outer diameter of the magnet ring B18 is smaller than that of the magnet ring A17, the inner diameter of the magnet ring B18 is the same as that of the magnet ring A17, the outer surface of the top end of the pipe section B9 is provided with a seal ring B19, and the seal ring B19 is arranged between the inner surface of the tail end of the pipe section A8 and the outer surface of the top end of the pipe section B9; the sealing ring B19 tightly connects the inner surface of the tail end of the pipe section A8 with the outer surface of the top end of the pipe section B9, so as to prevent liquid leakage; the filtering device 3 comprises a transparent polymer shell 20, the transparent polymer shell 20 is of a hollow structure, the transparent polymer shell 20 is made of polypropylene or polyether sulfone materials, a polymer filtering film layer 21, a sterilization film layer 22, a granular drying agent layer 23, an indication layer 24 and a reverse osmosis layer 25 are sequentially arranged in an inner cavity of the transparent polymer shell 20 from bottom to top, and the polymer filtering film layer 21, the sterilization film layer 22, the granular drying agent layer 23, the indication layer 24 and the reverse osmosis layer 25 are equidistantly arranged in the inner cavity of the transparent polymer shell 20 at intervals; the biological pollution to the tail end of the liquid suction pipe 7 and the influence on the experimental result caused by single structure and imperfect function of the filter element are fundamentally avoided; meanwhile, because the inner cavity of the filtering device 3 is provided with the multi-layer and multi-effect transparent composite biological safety filter element, the color-changing indicating function is realized, so that a user can be reminded to replace the pipette 7 and the filtering device 3 in time, the condition that the filtering device 3 is invalid and continues to be used is avoided, and the waste is avoided; the filtering device 3 has the functions of physical isolation, tiny dust prevention (including PM2.5), sterilization, bacteriostasis, pollution degree indication and accurate biological pollution prevention, thereby avoiding all known pollution to the maximum extent, simultaneously avoiding the condition that a user carries out non-standardized self-cleaning on the component after using the filtering device for a long time, and possibly causing damage and corrosion to the whole pipettor, in a molecular biology experiment, as simultaneously operating a plurality of possibly mutually polluted biological gene extract samples, the pipettor 7 can be replaced for a plurality of times in the operation process, thereby completely avoiding cross contamination, and avoiding systematic measurement deviation caused by simultaneously using a plurality of pipettors, so that the pipetting operation is more accurate and controllable, the experiment result, particularly the quantitative PCR experiment result related to genome research is more accurate and reliable, and the transparent polymer shell 20 is hard and transparent in texture, the color of the indicating layer 24 is convenient to change, so that the purpose of observation is achieved; the polymer filter membrane layer 21 is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer 22 comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular desiccant layer 23 comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indication layer 24 comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer 25 includes an acetate fiber film or an aromatic polyamide (hydrazide) film; the polymer filter membrane layer 21 is formed by stacking a plurality of nontoxic, harmless and environment-friendly artificial high polymer materials, so that the performance index of the polymer filter membrane layer 21 can reach the coverage aperture range of 0.22-0.45 mu m, the filtering effect can reach 99.9 percent, and the polymer filter membrane layer 21 achieves the aim of efficiently filtering organic pollutants such as micro-dust, aerosol and microorganisms in the air, mainly bacteria and large-particle organisms; the bactericidal membrane layer 22 is coated with silver ions, the active silver ions Ag2+ have high oxidation-reduction potential, and the biochemical reaction of bacterial protein is destroyed through the reaction with sulfhydryl on bacterial cell membranes; the bacteria are killed by the electronic transmission system of the active silver ion interference bacteria respiration, and the sterilization film layer 22 achieves the purpose of killing the bacteria leaked from the first filtering film layer; the granular drying agent layer 23 achieves the purpose of removing tiny moisture particles in liquid or air as far as possible, simultaneously reduces the adverse effect of any acid-base corrosive liquid on the part above the pipette filtering device 3 to the maximum extent, and prolongs the service life of the pipette 7 of the pipette; once moisture enters the indicating layer 24, anhydrous copper sulfate hydration reaction occurs immediately to generate blue copper sulfate pentahydrate, so that a user is instructed to replace the pipette 7 with the filtering device 3 immediately, and the user is prevented from judging whether to replace the pipette 7 with the filtering device 3 by personal assumption; the reverse osmosis layer 25 achieves the purpose of isolating heavy metal ions, colloids, viruses and biomacromolecules, and the layer film plays a role of isolating all proteins, RNA and DNA in liquid and environment as a last barrier; the membrane pore diameters of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber efficient filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane in the polymer filter membrane layer 21 are all 0.22-0.45 mu m, the membrane pore diameter of the polyethylene terephthalate membrane in the sterilization membrane layer 22 is xx-xxnm, the diameter of cobalt-free allochroic silica gel drying particles in the granular drying agent layer 23 is 0.5-1mm, the thickness of the granular drying agent layer 23 is 3-5mm, the thickness of the indication layer 24 is xx-xxmm, and the membrane pore diameter of an acetate fiber membrane or an aromatic polyamide (hydrazide) membrane is 0.5-10 nm.
A manufacturing method of a detachable pipettor with a filtering device comprises the following steps:
s1, manufacturing a filtering device 3 in a closed environment with the temperature of 10-25 ℃ and the humidity of 50-75% under atmospheric pressure, cutting any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane with the membrane aperture of 0.22-0.45 mu m to a circular sheet with the inner diameter of the transparent polymer shell 20, stacking the circular sheets to manufacture a polymer filter membrane layer 21, wherein the polymer filter membrane layer 21 is fixedly arranged at a position 1-2mm away from the bottom opening of the transparent polymer shell 20; then taking a polyethylene terephthalate film, cutting the polyethylene terephthalate film into a circular sheet with the inner diameter of the transparent polymer shell 20, coating silver ions on two surfaces of the polyethylene terephthalate film circular sheet to manufacture a sterilization film layer 22, and fixedly installing the sterilization film layer 22 above the polymer filter film layer 21; then taking four polypropylene films, cutting the four polypropylene films into a circular sheet with the inner diameter of the transparent polymer shell 20, fixedly installing the first polypropylene film above the sterilization film layer 22, paving cobalt-free allochroic silica gel dry particles with the size of 0.5-1mm on the first polypropylene film, leveling, paving the cobalt-free allochroic silica gel dry particles with the thickness of 3-5mm, fixedly installing the second polypropylene film on the leveled cobalt-free allochroic silica gel dry particles, and manufacturing into a granular desiccant layer 23; fixedly mounting a third polypropylene film above the granular desiccant layer 23, laying anhydrous copper sulfate powder with the thickness of 1-2mm on the third polypropylene film and leveling, and fixedly mounting a fourth polypropylene film on the leveled anhydrous copper sulfate powder to manufacture an indication layer 24; taking an acetate fiber film or an aromatic polyamide (hydrazide) film, cutting the acetate fiber film or the aromatic polyamide (hydrazide) film to a circular sheet with the inner diameter of the transparent polymer shell 20 to manufacture a reverse osmosis layer 25, and fixedly installing the reverse osmosis layer 25 above the indication layer 24;
s2, sterilizing the filter device 3 under the irradiation of gamma rays;
s3, installing the filter device 3 which is sterilized on the inner surface of the tail end of the B pipe section 9;
s4, connecting the pipe section B9 provided with the filtering device 3 with the pipe section A8 to form a pipette 7;
s5, connecting the pipette 7 to the pipette main body 1;
s6, the outer sleeve 6 is fitted around the outer surface of the pipette 7 and connected to the pipette main body 1.
The working principle of one embodiment of the invention is as follows: when the invention works, the pipe section A8 is connected with the pipe section B9, the installation of the pipette 6 is completed, the pipette 6 which is installed is placed in the outer sleeve 5, the pipe section C10 protrudes out of the tail end opening of the outer sleeve 5, the dust cap 26 is removed, the pipette nozzle 4 is installed on the outer surface of the pipe section C10, then, the liquid transferring operation is carried out, after the liquid transferring operation is finished, the color of the transparent polymer shell 20 in the filtering device 3 is checked, after the checking is finished, whether the pipe section B9 needs to be replaced or not is judged according to the color change, if the transparent polymer shell 20 is blue, indicating that the section B9 needs to be replaced, if the transparent polymer shell 20 is not blue, indicating that the section B9 can continue to be used, and, finally, the pipette tip release button 5 is pressed to make the pipette tip 4 fall off from the outer surface of the C pipe section 10, and the dust cap 26 is sleeved on the tail end of the outer sleeve 6.
Claims (8)
1. The detachable pipette with the filtering device is characterized by comprising a pipette main body (1), a connecting piece (2), the filtering device (3) and a liquid suction nozzle (4), wherein the pipette main body (1) is connected with the liquid suction nozzle (4) through the connecting piece (2), the filtering device (3) is arranged in an inner cavity of the connecting piece (2), the filtering device (3) is arranged at a position close to the connecting piece (2) and connected with the liquid suction nozzle (4), a liquid suction nozzle unloading button (5) is arranged on the pipette main body (1), the connecting piece (2) comprises a plurality of sections of pipe sections which are detachably connected, and the filtering device (3) is a transparent composite biological safety filter element device; the filtering device (3) comprises a transparent polymer shell (20), the transparent polymer shell (20) is of a hollow structure, the transparent polymer shell (20) is made of polypropylene or polyether sulfone materials, a polymer filter membrane layer (21), a sterilization membrane layer (22), a granular drying agent layer (23), an indication layer (24) and a reverse osmosis layer (25) are sequentially arranged in an inner cavity of the transparent polymer shell (20) from bottom to top, and the polymer filter membrane layer (21), the sterilization membrane layer (22), the granular drying agent layer (23), the indication layer (24) and the reverse osmosis layer (25) are arranged in the inner cavity of the transparent polymer shell (20) at equal intervals; the polymer filter membrane layer (21) is formed by stacking any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane; the sterilization film layer (22) comprises a polyethylene terephthalate film, and silver ions are coated on the polyethylene terephthalate film; the granular drying agent layer (23) comprises two polypropylene films, and cobalt-free allochroic silica gel drying granules are arranged between the two polypropylene films; the indicating layer (24) comprises two polypropylene films, and anhydrous copper sulfate powder is arranged between the two polypropylene films; the reverse osmosis layer (25) comprises an acetate fiber film or an aromatic polyamide film or an aromatic polyhydrazide film.
2. The detachable pipette with the filtering device according to claim 1, wherein the connecting member (2) comprises an outer sleeve (6) and a pipette (7), the outer sleeve (6) is sleeved on the outer surface of the pipette (7), the end of the pipette (7) protrudes out of the end of the outer sleeve (6), a dust cap (26) is arranged on the end of the outer sleeve (6), and one end of the pipette (7) protruding out of the end of the outer sleeve (6) is arranged in the dust cap (26).
3. The detachable pipette with the filtering device according to claim 2, wherein the pipette (7) comprises an A pipe section (8), a B pipe section (9) and a C pipe section (10), the tail end of the A pipe section (8) is detachably connected with the top end of the B pipe section (9), the inner diameter of the tail end of the A pipe section (8) is larger than the outer diameter of the top end of the B pipe section (9), the C pipe section (10) is arranged at the tail end of the B pipe section (9) and is connected with the pipette nozzle (4), the inner surface of the tail end of the A pipe section (8) is provided with a limiting ring (11), an A sealing ring (12) is arranged between the top end of the B pipe section (9) and the limiting ring (11), the diameter of the A sealing ring (12) is the same as that of the limiting ring (11), the inner diameter of the A sealing ring (12) is the same as that of the top end of the B pipe section (9), and the filtering device (3) is arranged at the inner surface of the tail end of the B pipe section (9), the inner surface of the liquid suction nozzle (4) is detachably connected with the outer surface of the C pipe section (10).
4. The detachable pipette with the filter device according to claim 3, wherein the inner surface of the end of the tube segment A (8) is provided with an internal thread (13), the outer surface of the top end of the tube segment B (9) is provided with an external thread (14) corresponding to the internal thread (13), and the internal thread (13) is in threaded connection with the external thread (14).
5. The detachable pipette with the filtering device according to claim 3, wherein the inner surface of the end of the tube segment A (8) is provided with a plurality of grooves (15), the outer surface of the top end of the tube segment B (9) is provided with a convex ring (16) corresponding to the grooves (15), and the grooves (15) are clamped with the convex ring (16).
6. The detachable pipette with the filtering device according to claim 3, wherein an A magnet ring (17) is arranged on the inner surface of the tail end of the A pipe section (8), a B magnet ring (18) is arranged on the top end of the B pipe section (9), the A magnet ring (17) is connected with the B magnet ring (18) in an adsorption mode, the diameter of the A magnet ring (17) is the same as that of the limiting ring (11), the outer diameter of the B magnet ring (18) is smaller than that of the A magnet ring (17), the inner diameter of the B magnet ring (18) is the same as that of the A magnet ring (17), a B sealing ring (19) is arranged on the outer surface of the top end of the B pipe section (9), and the B sealing ring (19) is arranged between the inner surface of the tail end of the A pipe section (8) and the outer surface of the top end of the B pipe section (9).
7. The detachable pipette with the filtering device according to claim 1, wherein the pore diameters of the polyvinylidene fluoride membrane, the polyethylene terephthalate membrane, the polyethersulfone modified microporous membrane, the polytetrafluoroethylene membrane, the mixed cellulose ester membrane or the polycarbonate microporous membrane are all 0.4-0.45 μm, the pore diameter of the polypropylene membrane is 40-50 μm, the pore diameter of the borosilicate glass fiber high-efficiency filter membrane is 0.22-0.45 μm, the diameter of the cobalt-free allochroic silica gel dry particles is 0.5-1mm, the thickness of the granular desiccant layer (23) is 3-5mm, the thickness of the indication layer (24) is 2-3mm, and the pore diameter of the acetate fiber membrane or the aromatic polyamide membrane or the aromatic polyhydrazide membrane is 0.5-10 nm.
8. A manufacturing method of a detachable pipettor with a filtering device is characterized by comprising the following steps:
s1, manufacturing a filtering device (3) in a closed environment with the temperature of 10-25 ℃ and the humidity of 50-75% under atmospheric pressure, cutting any two or more than two of a polyvinylidene fluoride membrane, a polypropylene membrane, a polyethylene terephthalate membrane, a borosilicate glass fiber high-efficiency filter membrane, a polyether sulfone modified microporous membrane, a polytetrafluoroethylene membrane, a mixed cellulose ester membrane or a polycarbonate microporous membrane into a circular sheet with the inner diameter of a transparent polymer shell (20), stacking the circular sheets to manufacture a polymer filter membrane layer (21), wherein the polymer filter membrane layer (21) is fixedly arranged at a position 1-2mm away from a bottom opening of the transparent polymer shell (20); then taking a polyethylene terephthalate film, cutting the polyethylene terephthalate film into a circular sheet with the inner diameter of the transparent polymer shell (20), coating silver ions on two surfaces of the polyethylene terephthalate film circular sheet to manufacture a sterilization film layer (22), and fixedly installing the sterilization film layer (22) above the polymer filter film layer (21); then taking four polypropylene films, cutting the four polypropylene films into a circular sheet with the inner diameter of the transparent polymer shell (20), fixedly installing the first polypropylene film above the sterilization film layer (22), paving cobalt-free allochroic silica gel dry particles with the size of 0.5-1mm on the first polypropylene film, leveling, paving the cobalt-free allochroic silica gel dry particles with the thickness of 3-5mm, fixedly installing the second polypropylene film on the leveled cobalt-free allochroic silica gel dry particles, and manufacturing into a granular drying agent layer (23); fixedly installing a third polypropylene film above the granular desiccant layer (23), paving anhydrous copper sulfate powder with the thickness of 1-2mm on the third polypropylene film, leveling, and fixedly installing a fourth polypropylene film on the leveled anhydrous copper sulfate powder to manufacture an indication layer (24); cutting an acetate fiber film or an aromatic polyamide film or an aromatic polyhydrazide film to a round sheet with the inner diameter of the transparent polymer shell (20) to manufacture a reverse osmosis layer (25), and fixedly installing the reverse osmosis layer (25) above the indication layer (24);
s2, sterilizing the filter device (3) under the irradiation of gamma rays;
s3, installing the filter device (3) which is sterilized on the inner surface of the tail end of the tube section B (9);
s4, connecting the pipe section B (9) provided with the filtering device (3) with the pipe section A (8) to form a liquid suction pipe (7);
s5, connecting the pipette (7) with the pipette main body (1);
and S6, sleeving the outer sleeve (6) on the outer surface of the pipette (7) and connecting the outer sleeve with the pipette main body (1).
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| CN202110404763.2A CN113058672B (en) | 2021-04-15 | 2021-04-15 | Detachable pipettor with filtering device and manufacturing method thereof |
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| CN202110404763.2A CN113058672B (en) | 2021-04-15 | 2021-04-15 | Detachable pipettor with filtering device and manufacturing method thereof |
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| CA1066674A (en) * | 1977-02-14 | 1979-11-20 | Gelman Instrument Company | Mouth filter for use with pipettes |
| US5058441A (en) * | 1989-12-18 | 1991-10-22 | The United States Of America As Represented By The Department Of Health And Human Services | Safety pipette and adaptor tip |
| US6123905A (en) * | 1997-01-17 | 2000-09-26 | Matrix Technologies Corporation | Pipettor including an indicator and method of use |
| US5851491A (en) * | 1997-06-13 | 1998-12-22 | Labcon, North America | Pipette tip and filter for accurate sampling and prevention of contamination |
| US7497994B2 (en) * | 1998-02-24 | 2009-03-03 | Khushroo Gandhi | Microfluidic devices and systems incorporating cover layers |
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| US7279134B2 (en) * | 2002-09-17 | 2007-10-09 | Intel Corporation | Microfluidic devices with porous membranes for molecular sieving, metering, and separations |
| US7231839B2 (en) * | 2003-08-11 | 2007-06-19 | The Board Of Trustees Of The Leland Stanford Junior University | Electroosmotic micropumps with applications to fluid dispensing and field sampling |
| DE102005005437A1 (en) * | 2005-02-05 | 2006-08-10 | Eppendorf Ag | Filter pipette tip |
| WO2007015048A1 (en) * | 2005-07-30 | 2007-02-08 | Otv Sa | Control apparatus for a liquid dispense system |
| US20070086924A1 (en) * | 2005-10-18 | 2007-04-19 | Drummond Scientific Company | Pipette with contamination indicator |
| US20080199361A1 (en) * | 2007-02-16 | 2008-08-21 | Francis Gomes | Hand-held pipetting device |
| WO2010093998A2 (en) * | 2009-02-14 | 2010-08-19 | Diffinity Genomics, Inc. | System and methods for purifying biological materials |
| DK2633914T4 (en) * | 2012-03-02 | 2020-06-08 | Eppendorf Ag | Pipetting device and method of manufacture thereof |
| CN202803267U (en) * | 2012-06-25 | 2013-03-20 | 谭俊 | Pipettor |
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