CN209010537U - Cell culture apparatus - Google Patents
Cell culture apparatus Download PDFInfo
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- CN209010537U CN209010537U CN201821554831.3U CN201821554831U CN209010537U CN 209010537 U CN209010537 U CN 209010537U CN 201821554831 U CN201821554831 U CN 201821554831U CN 209010537 U CN209010537 U CN 209010537U
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- cell culture
- culture apparatus
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- groove
- face
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Abstract
The utility model relates to a kind of cell culture apparatus, which includes glass slide and separating device, and multiple cell culture faces are formed on the upper surface of glass slide, and the edge in each cell culture face is respectively formed as edge groove；Separating device has multiple coaming plates interconnected, multiple coaming plates and multiple edge grooves correspond, the inside that the lower end of coaming plate is inserted into edge groove carries out enclosing to cell culture face, and is provided with seal member between the lower end of coaming plate and the inner wall of edge groove.Compared with cell climbing sheet culturing plate in the prior art, cell culture apparatus provided by the utility model has the advantages that convenient for disassembly and assembly, durable, easy cleaning disinfection.
Description
Technical field
The utility model relates to glass slide fields, more particularly, to a kind of cell culture apparatus.
Background technique
Cell cultivation in the prior art is mostly carried out by cell climbing sheet, and cell climbing sheet is exactly that slide is allowed to be immersed in cell training
It supports in base, cell is grown on slide, it is mainly used for histology, immunohistochemistry, frozen section, cell smear etc., but
Cell climbing sheet must be put into glass slide progress by subsequent experimental when observing, operation is more many and diverse, and misoperation also will cause cell
Creep plate pollution.
More mature scheme is a kind of adhesive type cell climbing sheet culturing plate in the prior art, i.e., viscous on the surface of glass slide
The orifice plate with open-work is pasted, cell is cultivated by the region that open-work and glass slide are formed, but when being bonded assembling, adhesive surface disappears
Poison is more troublesome, and is easily infected with impurity, and cell cultivation is caused to pollute, and adhesive surface use after a certain period of time will viscosity mistake
Effect, influences normal use.
The information disclosed in the background technology section is intended only to deepen the understanding to the utility model general background technology,
And it is not construed as recognizing or implying in any form that the information constitutes the prior art known in those skilled in the art.
Utility model content
The utility model is to propose in view of the above problems, and its purpose is to provide a kind of cell culture apparatus.
The cell culture apparatus have the advantages that it is convenient for disassembly and assembly, can Reusability, easy cleaning disinfection.
To realize that the purpose of this utility model adopts the following technical scheme that:
The utility model of first technical solution is a kind of cell culture apparatus, which includes glass slide and divide
Every device, multiple cell culture faces, the rim portion in each cell culture face are formed on the upper surface of the glass slide
Be not formed as, from the upper surface of the glass slide to edge groove made of the recess of the lower surface direction of the glass slide；
The separating device has multiple coaming plates interconnected, and multiple coaming plates and multiple edge grooves are one by one
Corresponding, in one group of mutual corresponding coaming plate and the edge groove: it is recessed that the lower end of the coaming plate is inserted into the edge
The inside of slot, to carry out enclosing to the cell culture face, also, in the lower end of the coaming plate and the edge groove
Seal member is provided between wall.
In addition, the cell culture apparatus of the second technical solution, on the basis of the cell culture apparatus of the first technical solution, institute
The cell culture face of stating is formed as rectangle or diamond shape.
In addition, the cell culture apparatus of third technical solution, more on the basis of the cell culture apparatus of the second technical solution
A edge groove is by a plurality of first straight line type groove being parallel to each other and a plurality of second straight line type groove structure being parallel to each other
At the angle between the extending direction of the first straight line type groove and the extending direction of the second straight line type groove is not equal to
Zero.
In addition, the cell culture apparatus of the 4th technical solution, on the basis of the cell culture apparatus of the second technical solution, respectively
The shape in a cell culture face, area are identical.
In addition, the cell culture apparatus of the 5th technical solution, on the basis of the cell culture apparatus of the first technical solution, institute
Stating seal member is spiral-lock in the U-shaped silicagel pad of the lower end of the coaming plate.
In addition, the cell culture apparatus of the 6th technical solution, on the basis of the cell culture apparatus of the first technical solution,
Poly-D-lysine coating is also coated on the cell culture face.
In addition, the cell culture apparatus of the 7th technical solution, on the basis of the cell culture apparatus of the first technical solution, institute
The bottom wall for stating the side wall and the edge groove of edge groove is mutually perpendicular to, and the lower end of the coaming plate is inserted vertically into the edge
The inside of groove.
In addition, the cell culture apparatus of the 8th technical solution, on the basis of the cell culture apparatus of the first technical solution, institute
Stating separating device also has the cover board for the upper end that can be covered in the coaming plate.
In addition, the cell culture apparatus of the 9th technical solution, on the basis of the cell culture apparatus of the 8th technical solution, institute
Cover board is stated to be made of transparent polypropylene.
In addition, the cell culture apparatus of the tenth technical solution, on the basis of the cell culture apparatus of the first technical solution, institute
Separating device is stated to be made up of polypropylene.
In conjunction with above technical scheme, the utility model bring beneficial effect is analyzed as follows:
The utility model provides a kind of cell culture apparatus, which includes glass slide and separating device,
Multiple cell culture faces are formed on the upper surface of glass slide, the edge in each cell culture face is respectively formed as, by load glass
Edge groove made of the upper surface of piece is recessed to the lower surface direction of glass slide；
Separating device has multiple coaming plates interconnected, and multiple coaming plates and multiple edge grooves correspond, at one group
In mutual corresponding coaming plate and edge groove: the lower end of coaming plate is inserted into the inside of edge groove, thus to cell culture face into
Row enclosing, also, seal member is provided between the lower end of coaming plate and the inner wall of groove.
In use, separating device to be plugged in above-mentioned edge groove first, the coaming plate of separating device is by cell culture face
Enclosing forms cell culturing space, and the clipping room of lower end sealing part the part sealed separation device and edge groove of coaming plate is arranged in
Gap prevents the cell culture fluid for instilling cell culturing space from the leakage of installation gap or cross contamination, when cell is trained in cell
Support look unfamiliar it is long after the completion of, separating device is removed from edge groove, then by the cell on the cell culture face for being grown in glass slide
After simple process, directly covered observation experiment can be carried out.
Compared with adhesive type cell climbing sheet culturing plate in the prior art, cell culture apparatus tool provided by the utility model
Have it is convenient for disassembly and assembly, can Reusability, easy cleaning disinfection the advantages of.
Other feature and advantage of the utility model will illustrate in the following description, also, partly from specification
In become apparent, or understood and implementing the utility model.The purpose of this utility model and other advantages are illustrating
Structure specifically noted by book, claims and attached drawing is achieved and obtained.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the overall structure diagram of the specific embodiment of cell culture apparatus provided by the utility model；
Fig. 2 is the assembly partial enlarged view of the separating device and seal member in Fig. 1.
Icon: 100- glass slide；110- edge groove；200- separating device；210- cover board；300- seal member.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
The concrete meaning of language in the present invention.
Below according to the structure of cell culture apparatus provided by the utility model, its specific embodiment is illustrated.
Fig. 1 is the overall structure diagram of the specific embodiment of cell culture apparatus provided by the utility model；Fig. 2 is figure
The assembly partial enlarged view of separating device and seal member in 1.
As depicted in figs. 1 and 2, a kind of cell culture apparatus is present embodiments provided, which includes carrying glass
Piece 100 and separating device 200, are formed with multiple cell culture faces on the upper surface of glass slide 100.
The edge in each cell culture face is respectively formed as, from the upper surface of glass slide 100 to the lower surface of glass slide 100
Edge groove 110 made of the recess of direction；Separating device 200 has multiple coaming plates interconnected, multiple coaming plates and multiple sides
Portion's groove 110 corresponds, and in one group of mutual corresponding coaming plate and edge groove 110: it is recessed that the lower end of coaming plate is inserted into edge
The inside of slot 110, to carry out enclosing to cell culture face, also, between the lower end of coaming plate and the inner wall of edge groove 110
It is provided with seal member 300.
Further, cell culture face is formed as rectangle or diamond shape.
Further, multiple edge grooves 110 are by a plurality of first straight line type groove being parallel to each other and a plurality of mutually flat
Capable second straight line type groove is constituted, between the extending direction of first straight line type groove and the extending direction of second straight line type groove
Angle be not equal to zero.
For example, the extending direction of first straight line type groove and second is directly in the case where cell culture face is formed as rectangle
Angle between the extending direction of line style groove is 90 degree；In the case where cell culture face is formed as diamond shape, first straight line type
Angle between the extending direction of groove and the extending direction of second straight line type groove is any degree between 0 degree to 90 degree,
For example, taking the angle between the extending direction of first straight line type groove and the extending direction of second straight line type groove is 30 degree.
In addition, the shape in each cell culture face, area are identical.
In addition, seal member 300 is spiral-lock in the U-shaped silicagel pad of the lower end of coaming plate.
In addition, being also coated with poly-D-lysine coating on cell culture face.
In addition, the side wall of edge groove 110 and the bottom wall of edge groove 110 are mutually perpendicular to, the lower end of coaming plate is inserted vertically into
To the inside of edge groove 110.
In addition, separating device 200 also has the cover board 210 for the upper end that can be covered in coaming plate.
Further, cover board 210 is made of transparent polypropylene.
In addition, separating device 200 is made up of polypropylene.
The specific structure of the utility model is illustrated above, illustrates its usage mode below.
Separating device when in use, is plugged in above-mentioned edge groove by above-mentioned cell culture apparatus first, separates dress
Cell culture face enclosing is formed cell culturing space by the coaming plate set, and the lower end sealing part part sealed separation dress of coaming plate is arranged in
The installation gap with edge groove is set, prevent the cell culture fluid for instilling cell culturing space from the leakage of installation gap or is intersected
Pollution.When cell is after the completion of cell culture looks unfamiliar long, separating device is removed from edge groove, and glass slide will be grown in
After cell simple process on cell culture face, can directly covered carry out observation experiment, have it is convenient for disassembly and assembly, can be anti-
The advantages of multiple use, easy cleaning sterilize.
In addition, cell culture face is formed as rectangle or diamond shape in above-mentioned specific embodiment.Cell culture face is set
It is set to rectangle or diamond shape, can reduce the processing and manufacturing difficulty that the edge groove of cell culture face edge is set, reduces production
Cost of manufacture.
In addition, multiple edge grooves are recessed by a plurality of first straight line type being parallel to each other in above-mentioned specific embodiment
Slot and a plurality of second straight line type groove being parallel to each other are constituted, the extending direction and second straight line type groove of first straight line type groove
Extending direction between angle be not equal to zero.
First straight line type groove and second straight line type groove the upper surface of glass slide can be divided to be formed it is multiple adjacent
Rectangle or diamond shape cell culture face improve the upper surface utilization rate to glass slide.
In addition, the shape in each cell culture face, area are identical in above-mentioned specific embodiment.By cell culture
Forming shape, the identical rectangle of area or diamond shape is arranged in face, and cell cultivation can be carried out on a cell cultivation device to according to the facts
It tests.For example, when experimental cell cultivates the influence situation of concentration versus cell growth of liquid, the cell culture of same shape, area
Face is it is avoided that cell grows influence of this variable of area to experimental result.
In addition, seal member is spiral-lock in the U-shaped silicagel pad of the lower end of coaming plate in above-mentioned specific embodiment.
As shown in Figure 2: U-shaped silicagel pad spiral-lock is fixed on the lower end of the coaming plate of separating device, and U-shaped silicagel pad can be in assembly
When separating device, the bottom surface and side of edge groove are abutted, improves leakproofness.
In addition, being also coated with poly-D-lysine coating on cell culture face in above-mentioned specific embodiment.Poly
Lysine coating can be improved the ability that cell culture is attached in face of the bonding of cell, promote cell raw on cell culture face
It is long.
In addition, the bottom wall of the side wall and edge groove of edge groove is mutually perpendicular in above-mentioned specific embodiment, enclose
The lower end of plate is inserted vertically into the inside of edge groove.Such structure being capable of easy disassembly coaming plate.
In addition, separating device also has the cover board for the upper end that can be covered in coaming plate in above-mentioned specific embodiment.
Cover board can be covered in the upper end of coaming plate during cell cultivation, form closed cell cultivation space, outer ring when preventing from cultivating
Border pollutes cell culture fluid.
In addition, cover board is made of transparent polypropylene in above-mentioned specific embodiment.Transparent polypropylene has excellent
The transparency and glossiness can facilitate growing state when observation cell cultivation, and polypropylene chemical property is stablized, manufactured
The disinfection of cover board resistant against high temperatures or the medicinal alcohol disinfection of 75% concentration.
In addition, separating device is made up of polypropylene in above-mentioned specific embodiment.Polypropylene chemical property is stablized,
With good toughness and elasticity, when inserting separating device, coaming plate and edge groove can be arranged to be interference fitted, improve and divide
Every device mounting stability, same polypropylene chemical property is stablized, and manufactured separating device resistant against high temperatures sterilize or 75% is dense
The medicinal alcohol of degree sterilizes.
The structure of specific embodiment of the utility model is illustrated above, but not limited to this.
For example, cell culture face is formed as rectangle or diamond shape in above-mentioned specific embodiment.
But not limited to this, and cell culture face can also be not configured to rectangle or diamond shape, is changed to be arranged to round or other shapes
Shape, the function of cell is cultivated in same achievable cell culture face, still, according to the structure of specific embodiment, by cell culture
Face is set as rectangle or diamond shape, can reach the processing and manufacturing difficulty for reducing edge groove and the beneficial effect for manufacturing cost
Fruit.
In addition, edge groove includes a plurality of first straight line type groove being parallel to each other in above-mentioned specific embodiment
With a plurality of second straight line type groove being parallel to each other.
But not limited to this, and above-mentioned edge groove is also possible to be formed by mutually independent multiple grooves, for example, working as cell
Culture face had not only had rectangle culture face, but also in the case where diamond shape culture face, groove is not limited to above-mentioned first straight line type
The structure of groove and second straight line type groove forms cell culture sky as long as can be realized and surround with edge groove and coaming plate
Between function.But according to the structure of specific embodiment, above-mentioned first straight line type groove and second straight line type are set
The upper surface of glass slide can be divided to form multiple adjacent rectangles or diamond shape cell culture face, improved to glass slide by groove
Upper surface utilization rate.
In addition, the shape in cell culture face, area are identical in above-mentioned specific embodiment.
But not limited to this, can not also limit shape, the area in cell culture face, when cell culture face had both had rectangle training
The face of supporting, and in the case where diamond shape culture face, the shape in each culture face is different from area；Alternatively, each cell training is arranged
It is identical to support face shape, for example, being rectangle, but the area between each cell culture face is different, cell culture equally can be achieved
The shape in cell culture face, area still according to the structure of specific embodiment, are arranged to phase by the function that cell is cultivated in face
Same rectangle or diamond shape, can carry out cell cultivation control experiment on a cell cultivation device, improve the utility model production
Product use function.
In addition, seal member is spiral-lock in the U-shaped silicagel pad of the lower end of coaming plate in above-mentioned specific embodiment.
But not limited to this, can also set seal member to be sprayed at applying with certain thickness rubber for coaming plate lower end
Layer, same achievable seal member is by the sealing function in the installation gap of separating device and edge groove, but rubber coating uses
It after a period of time, has and wears to a certain degree, reduce the sealing performance of seal member, in contrast, according to specific embodiment party
Seal member is set U-shaped silicagel pad by the structure of formula, can be improved the wear-resistant of seal member, improves the utility model and produces
The service life of product.
In addition, being also coated with poly-D-lysine coating on cell culture face in above-mentioned specific embodiment.
But not limited to this, and poly-D-lysine coating can not also be arranged on cell culture face, equally achievable cell training
Cultivate the function of cell in the face of supporting.But according to the structure of specific embodiment, poly-D-lysine is set on cell culture face and is applied
Layer, the ability that cell culture is attached in face of the bonding of cell that improves can be reached, what promotion cell was grown on cell culture face has
Beneficial effect.
In addition, the bottom wall of the side wall and edge groove of edge groove is mutually perpendicular in above-mentioned specific embodiment, enclose
The lower end of plate is inserted vertically into the inside of edge groove.
But not limited to this, can also set out of plumb for the side wall of edge groove and edge groove bottom wall, equally can be real
Existing edge groove is loaded the function of coaming plate.It is but according to the structure of specific embodiment, the side wall of edge groove and edge is recessed
Groove bottom wall is arranged vertically, and can reach the beneficial effect of easy disassembly coaming plate.
In addition, separating device also has the cover board for the upper end that can be covered in coaming plate in above-mentioned specific embodiment.
But not limited to this, and separating device can also be not provided with cover board, and equally achievable separating device is to cell cultivation face point
The function of cutting.But according to the structure of specific embodiment, separating device is provided with cover board, can reach outer ring when preventing from cultivating
The beneficial effect that border pollutes cell culture fluid.
In addition, cover board is made of transparent polypropylene in above-mentioned specific embodiment.
But not limited to this, and cover board can also be made of other materials, such as traditional translucent polypropylene, transparent glass,
The function in the closed cell cultivation space of same achievable cover board, still, according to the structure of specific embodiment, by cover board by saturating
Bright polypropylene is made, and can reach growing state when facilitating observation cell cultivation and the beneficial effect of disinfection easy to clean.
In addition, separating device is made up of polypropylene in above-mentioned specific embodiment.
But not limited to this, and separating device can also be made of other materials, such as glass, polystyrene, equally can be real
The function that existing separating device divides cell cultivation face.But according to the structure of specific embodiment, by separating device by poly- third
Alkene is made, and since polypropylene has good toughness and elasticity, can be arranged to be interference fitted by coaming plate and edge groove, improve and divide
While device mounting stability, disinfection also easy to clean.
In addition, the cell culture apparatus of the utility model, can be composed of, equally the various structures of above embodiment
Above-mentioned effect can be played.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (10)
1. a kind of cell culture apparatus, it is characterised in that: including glass slide and separating device,
Multiple cell culture faces are formed on the upper surface of the glass slide, the edge in each cell culture face distinguishes shape
Become, from the upper surface of the glass slide to edge groove made of the recess of the lower surface direction of the glass slide；
The separating device has multiple coaming plates interconnected, and multiple coaming plates and multiple edge grooves one are a pair of
It answers,
In one group of mutual corresponding coaming plate and the edge groove:
The lower end of the coaming plate is inserted into the inside of the edge groove, so that enclosing is carried out to the cell culture face, also,
Seal member is provided between the lower end of the coaming plate and the inner wall of the edge groove.
2. cell culture apparatus according to claim 1, it is characterised in that: the cell culture face is formed as rectangle or water chestnut
Shape.
3. cell culture apparatus according to claim 2, it is characterised in that: multiple edge grooves are by a plurality of mutually flat
Capable first straight line type groove and a plurality of second straight line type groove being parallel to each other are constituted, the extension of the first straight line type groove
Angle between direction and the extending direction of the second straight line type groove is not equal to zero.
4. cell culture apparatus according to claim 2, it is characterised in that: the shape in each cell culture face, face
Product is identical.
5. cell culture apparatus according to claim 1, it is characterised in that: the seal member is spiral-lock in the coaming plate
Lower end U-shaped silicagel pad.
6. cell culture apparatus according to claim 1, it is characterised in that: be also coated on the cell culture face more
Polylysine coating.
7. cell culture apparatus according to claim 1, it is characterised in that: the side wall of the edge groove and the edge
The bottom wall of groove is mutually perpendicular to, and the lower end of the coaming plate is inserted vertically into the inside of the edge groove.
8. cell culture apparatus according to claim 1, it is characterised in that: the separating device also has and can be covered in
The cover board of the upper end of the coaming plate.
9. cell culture apparatus according to claim 8, it is characterised in that: the cover board is made of transparent polypropylene.
10. cell culture apparatus according to claim 1, it is characterised in that:
The separating device is made up of polypropylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821554831.3U CN209010537U (en) | 2018-09-21 | 2018-09-21 | Cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821554831.3U CN209010537U (en) | 2018-09-21 | 2018-09-21 | Cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209010537U true CN209010537U (en) | 2019-06-21 |
Family
ID=66833973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821554831.3U Expired - Fee Related CN209010537U (en) | 2018-09-21 | 2018-09-21 | Cell culture apparatus |
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| Country | Link |
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| CN (1) | CN209010537U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115960814A (en) * | 2022-11-28 | 2023-04-14 | 江苏汇达医疗器械有限公司 | Multi-cavity cell culture slide and preparation process thereof |
-
2018
- 2018-09-21 CN CN201821554831.3U patent/CN209010537U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115960814A (en) * | 2022-11-28 | 2023-04-14 | 江苏汇达医疗器械有限公司 | Multi-cavity cell culture slide and preparation process thereof |
| CN115960814B (en) * | 2022-11-28 | 2023-10-31 | 江苏汇达医疗器械有限公司 | Multichamber cell culture slide and preparation process thereof |
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Granted publication date: 20190621 Termination date: 20210921 |