CN201459140U - Parallel-plate flowing small chamber with tiny ridges - Google Patents
Parallel-plate flowing small chamber with tiny ridges Download PDFInfo
- Publication number
- CN201459140U CN201459140U CN 200920110521 CN200920110521U CN201459140U CN 201459140 U CN201459140 U CN 201459140U CN 200920110521 CN200920110521 CN 200920110521 CN 200920110521 U CN200920110521 U CN 200920110521U CN 201459140 U CN201459140 U CN 201459140U
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- parallel
- cell
- plate
- parallel plate
- ridge
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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Abstract
The utility model relates to a parallel-plate flowing small chamber with tiny ridges which comprises an upper parallel plate and a lower parallel plate which are parallel, and a gasket arranged between the upper and the lower parallel plates, wherein the upper parallel plate is provided with a liquid inlet and a liquid outlet, wherein a cell attachment plate is installed in the region of the gasket on the lower parallel plate, gaps are left between the cell attachment plate and the upper parallel plate to form a small chamber, the surface of the cell attachment plate is provided with a plurality of rows of tiny ridges which are mutually parallel and are arranged with intervals, the tiny ridges are mutually parallel, and are arranged with intervals, the cross section of the tiny ridge is in a trapezoid, and the height of the tiny ridge is 40 mum, and the distance between the two tiny ridges is 100 mum. Through arranging the tiny ridges for regulating the flowing state in the small chamber, the partial turbulence is avoided forming in the small chamber, and the parallel-plate flowing small chamber can be widely applied in irrigation systems for researching vessel esoderma cells cultured in vitro to shearing force, and can be used as an experiment device in rheologic researches.
Description
Technical field:
The utility model belongs to the experimental installation field, is specifically related to a kind of experimental installation parallel plate flow cell of studying cell response.
Background technology:
The parallel plate flow cell, be called for short stream chamber (flow chamber), it is one of device the most frequently used in the Research on The Rheology, generally be assembled in the perfusion system of research cultured vascular endothelial cells to shearing stress and use, the stream chamber system can also be used to study the pathogenesis of inflammation or tumour, such as: leukocytic the oozing out and migration of application such as Nakhaei-Neiad M stream chamber system research, Yoo PS etc. use the lymphatic metastasis of stream chamber system research colorectal carcinoma.
The dull and stereotyped up and down slit that forms is passed through in existing stream chamber, and flat board generally has projection or groove up and down, forms embedded model, and is very high to the requirement of working accuracy, the very difficult homogeneity that guarantees slit height.Existing improved stream chamber, flat board needn't be established projection or groove up and down, establish packing ring at dull and stereotyped edge up and down, the installing cell climbing sheet has formed a kind of stream chamber of novel structure in the packing ring, and the parallel plate flow cell that this is multi-form can satisfy various reaction system needs flexibly, blemish in an otherwise perfect thing be, cultured cells during adherent growth, lack of alignment at random can occur on parallel plate, arrange along the blood vessel major axis at body with endotheliocyte not conform to.In addition, form turbulent flow in the part easily at cell climbing sheet interface microcell in the parallel plate design.These problems are all brought some uncertainties to experimental result.
Summary of the invention:
The purpose of this utility model is to provide a kind of endotheliocyte major axis streamwise ordered arrangement that can make to realize simulating the real microscale fluid environment of organism, thereby and can obtain a large amount of purpose cells and improve the drawback that some micro device culturing cell amount pettiness bring, and can avoid forming the parallel plate flow cell that local microcell turbulent has little ridge.
Parallel plate flow cell of the present utility model, be provided with the upper flat plate and the following flat board that are parallel to each other, be provided with packing ring along the edge therebetween, the dull and stereotyped down installing cell climbing sheet of going up in the packing ring, cell climbing sheet is provided with little ridge that number row parallel distance is arranged, leave the space between little ridge of cell climbing sheet and the upper flat plate, establish the fluid inlet and the liquid outlet that feed this space on the upper flat plate.
Wherein, the cross section of described little ridge is trapezoidal.
The height of described little ridge is 40 μ m.
Spacing is 100 μ m between described two little ridges.
In the described parallel plate flow cell, but cell climbing sheet multi-disc level stacks.
In the described parallel plate flow cell, the gap adds small washer between cell climbing sheet and the upper flat plate.
When being provided with a plurality of cell climbing sheet, described small washer is added in gap between top layer cell climbing sheet and the upper flat plate.
Adopt above technical scheme, parallel plate flow cell of the present utility model, owing to adopt the prominent cell climbing sheet of band ridge, capillary endothelium is being arranged in the substrate between two ridges by flow direction, can make endotheliocyte major axis streamwise ordered arrangement realize simulating the real microscale fluid environment of organism, and apply the shearing of flowing, obtain a large amount of purpose cells.Parallel plate flow cell of the present utility model both can realize simulating the microscale fluid environment, can avoid local turbulence to form by the flow state in little ridge standard cell again.
Description of drawings:
Fig. 1 is the utility model parallel plate flow cell schematic cross-section.
Fig. 2 is the microcosmic stereographic map of cell climbing sheet in the parallel plate flow cell.
Fig. 3 is a parallel plate flow cell upper surface synoptic diagram.
Embodiment:
Referring to shown in Figure 1, the utility model parallel plate flow cell comprises the upper flat plate 11 and following dull and stereotyped 12 that is parallel to each other, between upper and lower flat board, be provided with packing ring 13, install cell climbing sheet 14 in dull and stereotyped 12 upper gaskets, 13 inner compartments down, leave the space between cell climbing sheet 14 and the upper flat plate 11 and form cell, establish fluid inlet 17 and liquid outlet 18 feeding cells (as shown in Figure 3) on the upper flat plate 11.
In conjunction with shown in Figure 2, cell climbing sheet 14 surfaces are provided with upwards little ridge 15 of protuberance of number row, this slightly ridge 15 spacing that is parallel to each other arrange, little ridge 15 cross sections are preferably trapezoidal (up-small and down-big) or analogous shape, the height of little ridge is preferably 40 μ m, and spacing is preferably 100 μ m between two little ridges 15.The utility model is by being provided with the flow state of little ridge in can the standard cell, avoids that local turbulence forms in the cell.
In the utility model, cell climbing sheet 14 can be monolithic, and is floating for preventing to place the cell climbing sheet of packing ring 13 inside, can adopt the thickness small washer 16 fixed cell creep plates 14 identical with parallel plate flow cell height; Cell climbing sheet 14 also can be established multi-disc, and every level stacks, and this moment, small washer 16 was placed between top layer cell climbing sheet 14 and the upper flat plate 11.
In the utility model, the material of upper and lower flat board is generally polycarbonate, synthetic glass or stainless steel.The polycarbonate transparent degree is good, and is wear-resisting, can high temperature, autoclave sterilization, but be difficult for pasting.Synthetic glass is pasted easily, but non-refractory, high pressure can adopt the uv irradiating sterilization.Stainless steel hardness is big, can help dull and stereotyped up and down effective the fixing of realizing.Select a certain or two kinds of materials according to sterilization, needs fixing and that observe.
The material of packing ring can be selected silica gel or poly-tetrafluoro thin slice, selects different thickness as required.Silica gel sheet sealing effectiveness is better, but elasticity is bigger, causes highly inhomogeneous in the process of fixed flow chamber easily.Poly-tetrafluoro sheet can be realized sealing, can avoid highly inhomogeneous again.
Claims (7)
1. parallel plate flow cell with little ridge, comprise the upper flat plate that is parallel to each other, the following dull and stereotyped and packing ring between upper and lower flat board, establish fluid inlet and liquid outlet on the upper flat plate, it is characterized in that, install cell climbing sheet in the dull and stereotyped upper gasket inner compartment down, leave the space between cell climbing sheet and the upper flat plate and form cell, the cell climbing sheet surface is provided with the little ridge to upper process that several capable spacings that are parallel to each other are arranged.
2. according to the described parallel plate flow cell of claim 1, it is characterized in that the cross section of described little ridge is up-small and down-big trapezoidal with little ridge.
3. according to the described parallel plate flow cell of claim 1, it is characterized in that the height of described little ridge is 40 μ m with little ridge.
4. according to the described parallel plate flow cell of claim 1, it is characterized in that spacing is 100 μ m between described two little ridges with little ridge.
5. according to the arbitrary described parallel plate flow cell of claim 1 to 4, it is characterized in that the gap adds small washer between described cell climbing sheet and the upper flat plate with little ridge.
6. according to the arbitrary described parallel plate flow cell of claim 1 to 4, it is characterized in that described cell climbing sheet is the multi-disc that level stacks with little ridge.
7. according to the described parallel plate flow cell of claim 6, it is characterized in that the gap adds small washer between top layer creep plate and upper flat plate with little ridge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920110521 CN201459140U (en) | 2009-07-27 | 2009-07-27 | Parallel-plate flowing small chamber with tiny ridges |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920110521 CN201459140U (en) | 2009-07-27 | 2009-07-27 | Parallel-plate flowing small chamber with tiny ridges |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201459140U true CN201459140U (en) | 2010-05-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200920110521 Expired - Lifetime CN201459140U (en) | 2009-07-27 | 2009-07-27 | Parallel-plate flowing small chamber with tiny ridges |
Country Status (1)
| Country | Link |
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| CN (1) | CN201459140U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880628A (en) * | 2010-07-14 | 2010-11-10 | 中国人民解放军第四军医大学 | Cell culture dish for constant-volume perfusion |
| CN117305102A (en) * | 2023-11-10 | 2023-12-29 | 中南大学 | Acoustic flow control device for sorting outer vesicles in plasma sample and use method thereof |
-
2009
- 2009-07-27 CN CN 200920110521 patent/CN201459140U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880628A (en) * | 2010-07-14 | 2010-11-10 | 中国人民解放军第四军医大学 | Cell culture dish for constant-volume perfusion |
| CN101880628B (en) * | 2010-07-14 | 2012-05-30 | 中国人民解放军第四军医大学 | Cell culture dish for constant-volume perfusion |
| CN117305102A (en) * | 2023-11-10 | 2023-12-29 | 中南大学 | Acoustic flow control device for sorting outer vesicles in plasma sample and use method thereof |
| CN117305102B (en) * | 2023-11-10 | 2024-05-14 | 中南大学 | Acoustic flow control device for sorting outer vesicles in plasma sample and use method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100512 |