CN201330249Y - Parallel board flow chamber perfusion system - Google Patents
Parallel board flow chamber perfusion system Download PDFInfo
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- CN201330249Y CN201330249Y CN 200920001501 CN200920001501U CN201330249Y CN 201330249 Y CN201330249 Y CN 201330249Y CN 200920001501 CN200920001501 CN 200920001501 CN 200920001501 U CN200920001501 U CN 200920001501U CN 201330249 Y CN201330249 Y CN 201330249Y
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- flow cell
- parallel plate
- cell
- plate flow
- upper flat
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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Abstract
The utility model provides a parallel board flow chamber perfusion system which comprises a gas cylinder, a buffer bottle, a parallel board flow chamber and a peristaltic pump that are connected with the pipe, wherein the buffer bottle is placed in a water bath. The parallel board flow chamber is placed on a heating cushion that is controlled by a temperature controller. An air valve is installed on the connecting pipe between the gas cylinder and the buffer bottle. According to the utility model, the stabilization of air current is controlled through an air valve. Two modes of the water bath and the heating cushion are adopted for controlling the temperature and heating the buffer bottle and the parallel board flow chamber. The validity of temperature controlling can be guaranteed. The peristaltic pump is adopted and the pulsation under the physiologic state can be simulated. The parallel board flow chamber perfusion system of the utility model can be widely used for real-time recording and observing the effect of different flowing forms to the cell, and can be used as an experimental device in the rheology researching.
Description
Technical field:
The utility model belongs to the experimental installation field, is specifically related to a kind of experimental installation of studying cell response.
Background technology:
The parallel plate flow cell is called for short stream chamber (flow chamber), is one of device the most frequently used in the Research on The Rheology.Based on the perfusion system of parallel plate flow cell, be called for short the stream chamber system, in of the reaction of research cultured vascular endothelial cells, be widely used to shearing stress.Studies show that the stream chamber system can also be used to study the pathogenesis of inflammation or tumour.Such as, leukocytic the oozing out and migration of application such as Nakhaei-Nejad M stream chamber system research.Yoo PS etc. use the lymphatic metastasis of stream chamber system research colorectal carcinoma.
The dull and stereotyped up and down slit that forms is passed through in existing stream chamber, and flat board generally has projection or groove up and down, forms embedded model, and is very high to the requirement of working accuracy, the very difficult homogeneity that guarantees slit height.The stream chamber system is made up of stream chamber, circulation power feeding mechanism, heating unit and gas supply device.Adopt water-bath that surge flask is heated, the unification of temperature and stable in the very difficult assurance system.Adopt the direct supply gas of gas cylinder, be difficult to guarantee the constant of air-flow.
Summary of the invention:
The purpose of this utility model is to provide a kind of can the simulation under the physiological status to pulse, and effectively controls the parallel plate flow cell perfusion system of air-flow and temperature.
Parallel plate flow cell perfusion system of the present utility model, be connected with pipeline with peristaltic pump by gas cylinder, surge flask, parallel plate flow cell successively, wherein, surge flask places in the water bath, and the parallel plate flow cell places on the heating cushion of being controlled by temperature regulator.
Wherein, on gas cylinder and surge flask connecting pipeline, establish an air valve.
Wherein, the gas cylinder pipeline feeds under the liquid level of surge flask bottom, and another pipeline that stretches into liquid level is communicated with the liquid-inlet pipe of parallel plate flow cell, and the drain pipe of parallel plate flow cell is communicated with peristaltic pump, and another pipeline of peristaltic pump feeds on the interior liquid level of surge flask.
In the above parallel plate flow cell perfusion system, described parallel plate flow cell is provided with upper flat plate and the following flat board that is parallel to each other, be provided with packing ring along the edge therebetween, installing cell climbing sheet in the packing ring, leave the space between cell climbing sheet and the upper flat plate, establish the fluid inlet and the liquid outlet that feed this space on the upper flat plate.
The gap adds small washer between described cell climbing sheet and the upper flat plate.Cell climbing sheet can be multi-disc, and described small washer is added in gap between top layer creep plate and the upper flat plate.
In the above parallel plate flow cell perfusion system, described parallel plate flow cell is provided with upper flat plate and the following flat board that is parallel to each other, establish cell climbing sheet on the following flat board, establish packing ring between cell climbing sheet and the upper flat plate, establish the fluid inlet and the liquid outlet that feed the packing ring internal pore on the upper flat plate.
Adopt above technical scheme, parallel plate flow cell perfusion system of the present utility model, the installing air valve can be controlled the stable of air-flow; Adopt water-bath and heating cushion dual mode controlled temperature, respectively surge flask and parallel plate flow cell are heated, can guarantee temperature controlled validity; Adopt peristaltic pump, can simulate the pulsation under the physiological status; Multi-form parallel plate flow cell can satisfy various reaction system needs flexibly.The whole system collaborative work, but real time record and the influence of observing different liquid form pair cells can be used as the experimental installation in the Research on The Rheology.
Description of drawings:
Fig. 1 is the utility model parallel plate flow cell perfusion system pie graph;
Fig. 2 is the schematic cross-section of a kind of parallel plate cell in the parallel plate flow cell perfusion system;
Fig. 3 is the schematic cross-section of another kind of parallel plate cell in the parallel plate flow cell perfusion system;
Fig. 4 is a parallel plate cell upper surface synoptic diagram in the parallel plate flow cell perfusion system.
Embodiment:
Referring to shown in Figure 1, the utility model parallel plate flow cell perfusion system is made up of gas cylinder 1, air valve 2, water bath 3, surge flask 4, temperature regulator 5, heating cushion 6, parallel plate flow cell 7, peristaltic pump 9 and connection line 8 etc.
Wherein, surge flask 4 places in the water bath 3, air valve 2 is located at and is used to control the stable of air-flow on gas cylinder 1 pipeline, gas cylinder 1 pipeline feeds surge flask 4 bottoms, surge flask 4 is communicated with the liquid-inlet pipe 71 of parallel plate flow cell 7 by a pipeline that stretches into liquid level (perfusate), the drain pipe 72 of parallel plate flow cell 7 is communicated with peristaltic pump 9, and peristaltic pump 9 another pipelines feed on the surge flask 4 interior liquid levels; Parallel plate flow cell 7 places on the heating cushion 6, and heating cushion 6 is connected with temperature regulator 5 by circuit, by temperature regulator 5 controls.
In the system, the gas in the gas cylinder 1 is CO
2(5%) and the mixed gas of air (95%).Air valve 2 can be controlled the stable of air-flow.Adopt water bath 3 and heating cushion 6 dual mode controlled temperature, respectively surge flask 4 and parallel plate flow cell 7 are heated, can guarantee temperature controlled validity.Adopt peristaltic pump 9, can simulate the pulsation under the physiological status.
Parallel plate flow cell 7 can have two kinds of structures in the utility model, as shown in Figure 2 a kind of, between the upper flat plate 11 that is parallel to each other and following dull and stereotyped 12, be provided with packing ring 13, installing cell climbing sheet 14 in the packing ring 13, leave the space between cell climbing sheet 14 and the upper flat plate 11 and form cell, establish fluid inlet 71 and liquid outlet 72 feeding cells (as shown in Figure 4) on the upper flat plate 11.
Another kind of parallel plate flow cell 7 structures as shown in Figure 3, upper flat plate 11 and following dull and stereotyped 12 is parallel to each other, establish cell climbing sheet 14 on following dull and stereotyped 12, establish packing ring 13 between cell climbing sheet 14 and the upper flat plate 11, packing ring 13 internal pores form cell, establish fluid inlet 71 and liquid outlet 72 feeding cells (as shown in Figure 4) on the upper flat plate 11.
In the utility model, if cell climbing sheet 14 has only a slice, can select to be positioned in the packing ring 13 or the below dual mode, no matter which kind of mode can both prevent fluid seepage; If cell climbing sheet 14 more than a slice, can only be selected to be positioned in the packing ring 13.Floating for preventing to place the cell climbing sheet of packing ring 13 inside, can adopt thickness small washer 15 (referring to Fig. 2) the fixed cell creep plate 14 identical with parallel plate flow cell height.
In the utility model, the material of upper and lower flat board is generally polycarbonate, synthetic glass or stainless steel.The polycarbonate transparent degree is good, and is wear-resisting, can high temperature, autoclave sterilization, but be difficult for pasting.Synthetic glass is pasted easily, but non-refractory, high pressure can adopt the uv irradiating sterilization.Stainless steel hardness is big, can help dull and stereotyped up and down effective the fixing of realizing.Select a certain or two kinds of materials according to sterilization, needs fixing and that observe.
The material of packing ring can be selected silica gel or poly-tetrafluoro thin slice, selects different thickness as required.Silica gel sheet sealing effectiveness is better, but elasticity is bigger, causes highly inhomogeneous in the process of fixed flow chamber easily.Poly-tetrafluoro sheet can be realized sealing, can avoid highly inhomogeneous again.
The utility model can be used in following several respects:
1. real time record and observe of the effect of different liquid forms such as laminar flow, turbulent flow to red corpuscle, thrombocyte, white corpuscle, endotheliocyte or tumour cell etc.;
2. study form, function and the biological characteristics variation in the flow field of various cells, for inquiring into the pathogenesis service of cardiovascular disorder, inflammation and tumour etc.
Claims (7)
1, a kind of parallel plate flow cell perfusion system, it is characterized in that, be connected with pipeline with peristaltic pump by gas cylinder, surge flask, parallel plate flow cell successively, wherein, surge flask places in the water bath, and the parallel plate flow cell places on the heating cushion of being controlled by temperature regulator.
2, according to the described parallel plate flow cell of claim 1 perfusion system, it is characterized in that, on gas cylinder and surge flask connecting pipeline, establish an air valve.
3, according to the described parallel plate flow cell of claim 1 perfusion system, it is characterized in that, the gas cylinder pipeline feeds under the liquid level of surge flask bottom, another pipeline that stretches into liquid level is communicated with the liquid-inlet pipe of parallel plate flow cell, the drain pipe of parallel plate flow cell is communicated with peristaltic pump, and another pipeline of peristaltic pump feeds on the interior liquid level of surge flask.
4, according to claim 1 or 2 or 3 described parallel plate flow cell perfusion systems, it is characterized in that, described parallel plate flow cell is provided with upper flat plate and the following flat board that is parallel to each other, be provided with packing ring along the edge therebetween, installing cell climbing sheet in the packing ring, leave the space between cell climbing sheet and the upper flat plate, establish the fluid inlet and the liquid outlet that feed this space on the upper flat plate.
5, according to the described parallel plate flow cell of claim 4 perfusion system, it is characterized in that the gap adds small washer between described cell climbing sheet and the upper flat plate.
6, according to claim 1 or 2 or 3 described parallel plate flow cell perfusion systems, it is characterized in that, described parallel plate flow cell is provided with upper flat plate and the following flat board that is parallel to each other, establish cell climbing sheet on the following flat board, establish packing ring between cell climbing sheet and the upper flat plate, establish the fluid inlet and the liquid outlet that feed the packing ring internal pore on the upper flat plate.
According to the described parallel plate flow cell of claim 5 perfusion system, it is characterized in that 7, cell climbing sheet is a multi-disc, described small washer is added in gap between top layer creep plate and the upper flat plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920001501 CN201330249Y (en) | 2009-01-05 | 2009-01-05 | Parallel board flow chamber perfusion system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920001501 CN201330249Y (en) | 2009-01-05 | 2009-01-05 | Parallel board flow chamber perfusion system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201330249Y true CN201330249Y (en) | 2009-10-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200920001501 Expired - Fee Related CN201330249Y (en) | 2009-01-05 | 2009-01-05 | Parallel board flow chamber perfusion system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628307A (en) * | 2018-11-07 | 2019-04-16 | 南昌市第医院 | The experimental facilities and method of hydrodynamics environment in a kind of analogue body |
| CN110583626A (en) * | 2019-09-26 | 2019-12-20 | 四川大学华西医院 | Multichannel constant-temperature perfusion system and application method thereof |
-
2009
- 2009-01-05 CN CN 200920001501 patent/CN201330249Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628307A (en) * | 2018-11-07 | 2019-04-16 | 南昌市第医院 | The experimental facilities and method of hydrodynamics environment in a kind of analogue body |
| CN110583626A (en) * | 2019-09-26 | 2019-12-20 | 四川大学华西医院 | Multichannel constant-temperature perfusion system and application method thereof |
| CN110583626B (en) * | 2019-09-26 | 2021-07-30 | 四川大学华西医院 | Multichannel constant-temperature perfusion system and application method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091021 Termination date: 20140105 |