CN207210446U - A kind of fluid flow damper applied to cell culture chamber - Google Patents
A kind of fluid flow damper applied to cell culture chamber Download PDFInfo
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- CN207210446U CN207210446U CN201720630758.2U CN201720630758U CN207210446U CN 207210446 U CN207210446 U CN 207210446U CN 201720630758 U CN201720630758 U CN 201720630758U CN 207210446 U CN207210446 U CN 207210446U
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- flow
- flow damper
- fluid
- port connector
- damper housing
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Abstract
A kind of fluid flow damper applied to cell culture chamber, the flow damper is provided with the fluid intake (1) that bore is 10mm, bore is 6mm fluid issuing (2) and the flow damper housing (3) of PVC material, described flow damper housing (3) inner bay is provided with three layers of supporting plate (4), through hole (5) is offered on supporting plate (4), described supporting plate (4) if on be provided with the unhurried current plate (6) that dry thickness is 3mm, described fluid intake (1) place is provided with inlet port connector (7), inlet port connector (7) is threadedly attached to the fixed seat (11) on flow damper housing (3), reinforcement (12) is provided with the outside of described fixed seat (11), described fluid issuing (2) place is provided with outlet port connector (8), liquid level detector (14) is additionally provided with described flow damper.The flow damper can effectively buffer the rate of flow of fluid and flow pumped out from peristaltic pump, be easy to flow control valve fluid flow and flow velocity to be finely adjusted, and lift the reliability of its simulation.
Description
Technical field
It the utility model is related to a kind of fluid flow damper applied to cell culture chamber.
Background technology
The research of cell micro-environment and cell interaction has become the study hotspot of cell biology.Structure is thin
The hydrodynamics and biological chemical environment of born of the same parents in vitro (in vitro) growth be Study of Fluid mechanics and biological chemical environment with it is thin
The premise of born of the same parents' interaction.Highly much smaller than horizontal and vertical physical dimension and size is in the flat of micron, millimeter or centimetres
Row flat flow chamber is one of Main Means for building cell fluid mechanics and biological chemical environment, particularly thin in biomethanics
Born of the same parents' biomethanics is widely used in being worked with power biological study.However, the parallel flat flowing that people are usually used
Chamber is fixed by multiple screws, and installation and removal process is complicated, causes lengthen experimental period, the defects of cell is easily damaged.Compel
It is essential and wants a kind of attaching/detaching process to reduce cellular damage and the novel parallel sheet flow of energy online observation conveniently, as far as possible
Chamber device.
Mechanical environment is an essential condition of cell development, hydrodynamic shear be a kind of wherein important mechanics because
Element.Fluid shearing method is that research cell is in shearing masterpiece using shear stress caused by nutrient solution flowing to cell surface
With lower form, stick changing rule with function etc..According to the difference for making fluid produce the type of flow, fluid shearing method can divide
For：Parallel flat flow chamber (parallel plate f1owchamber, PPFC), cone-plate formula flow cavity, the flowing of the plate of plate one
Chamber, cylindrical tube and radial flow arrangement etc..Wherein, parallel flat flow chamber is easy in microscope carrier due to flow chamber small volume
Upper Real Time Observation, display and record, and installation operation is easy quick, the advantages that easily realizing Automated condtrol, flowed in research
Body shearing force is very wide on purposes in terms of cytosis influence.For example, vascular endothelial cell, Gegenbaur's cell are made in fluid shear stress
There is application with waiting in research.
Be made up of dynamical system, flow cavity device and observation system three parts one of parallel flat flow chamber system follows
Loop back path system.The driving force of steady or transient is provided by dynamical system, drives nutrient solution to flow in systems, due to culture
The viscous effect of liquid, the cell for making to be adhered to flow chamber bottom are passed through microscopic observation by constant or pulsation shearing force
And image capture system, the changing rule of observation or detection cell under shear stress.In parallel flat flow chamber system
In, flow cavity device is the core of whole system.At present, in organizational project flow cavity device flow chamber, mainly
By very thin between two blocks of parallel-plates of two pieces of parallel flat boards (i.e. upper flat plate, lower flat board) of water white transparency and one piece
The silica gel sealing ring composition of sealing function, make upper flat plate, lower flat board and silica gel sealing by way of screw or use vacuumize
Circle is closely attached together, so as to surround the very big square-section flow chamber of a ratio of width to height jointly.The two of upper flat plate
Side has aperture or slit, and circulation fluid flows into and out from this two opening；And lower flat board is then the carrier of cell.When following
When ring liquid flows in this chamber, more uniform hydrodynamic shear can be produced, but in actual applications, simulates the stream of intravital blood
Body under the pump of peristaltic pump penetrates power inject upper flat plate in aperture when due to flow control valve effect hysteresis quality, often in fluid
Enter that fashionable impulsive force is uncontrollable, and then influence the regulation and control to shearing force.
Therefore, it is necessary to be improved to existing equipment, the fluid that peristaltic pump pumps out further is buffered, allow
Rate of flow of fluid, flow become uniform, and then it is further finely tuned by flow control valve, so as to ensure that shearing force is simulated
Reliability.
The content of the invention
In order to overcome the above-mentioned deficiencies of the prior art, the utility model proposes a kind of fluid applied to cell culture chamber
Flow damper, the flow damper can effectively buffer the rate of flow of fluid and flow pumped out from peristaltic pump, be easy to flow control valve convection body stream
Amount and flow velocity are finely adjusted, and lift the reliability of its simulation.
In order to achieve the above object, the utility model proposes following technical scheme：
A kind of described fluid flow damper applied to cell culture chamber, the flow damper are provided with the fluid that bore is 10mm
The flow damper housing (3) of entrance (1), the fluid issuing (2) that bore is 6mm and PVC material, described flow damper housing (3)
Inner bay is provided with three layers of supporting plate (4), and through hole (5) is offered on supporting plate (4), described supporting plate (4) if on be provided with dry thickness and be
3mm unhurried current plate (6), described fluid intake (1) place are provided with inlet port connector (7), and inlet port connector (7) is connected through a screw thread
Fixed seat (11) on to flow damper housing (3), described fixed seat (11) outside are provided with reinforcement (12), and described fluid goes out
Mouth (2) place is provided with outlet port connector (8), and liquid level detector (14) is additionally provided with described flow damper.
Further, the junction of described fixed seat (11) and flow damper housing (3) is provided with the collection that two bores are 3mm
Press mouth (9), flow damper housing (3) it is corresponding with collection pressure mouth (9) be oblique angle for 60 degree of horn-like spout (10), described goes out
The junction of mouth connector (8) and flow damper housing (3) is provided with the funnel-form that oblique angle is 60 degree and exports (13).
Further, described unhurried current plate (6) is cellular that it is some for 1mm through hole to which is provided with aperture.
The beneficial effects of the utility model：The utility model proposes a kind of fluid unhurried current applied to cell culture chamber
Device, the flow damper are located between peristaltic pump and flow control valve, which is provided with cellular unhurried current plate, can effectively be reduced due to wriggling
Pump pumps out the temporary impact of fluid so that by the rate of flow of fluid and flow held stationary of flow control valve, greatly promotes fluid
The reliability of environmental simulation.
Brief description of the drawings
Fig. 1 is existing cell culture room environmental overall structure diagram；
Fig. 2 is the utility model fluid entirety control structure schematic diagram；
Fig. 3 is the structural representation of the utility model flow damper；
Fig. 4 is the cellular unhurried current chip architecture schematic diagram of the utility model flow damper.
Embodiment
In order that the purpose of this utility model, technical scheme and beneficial effect are clearer, below in conjunction with accompanying drawing 1-4,
Preferred embodiment of the present utility model is described in detail, to facilitate the technical staff to understand.
As in Figure 2-4, in Fig. 2, peristaltic pump 16 is connected on flow damper 17, and flow damper 17 is connected to flow control valve
18, a kind of described fluid flow damper applied to cell culture chamber, the flow damper is provided with the fluid intake that bore is 10mm
1st, bore is 6mm fluid issuing 2 and the flow damper housing 3 of PVC material, and the described inner bay of flow damper housing 3 is provided with three
Layer supporting plate 4, offers through hole 5 on supporting plate 4, if being provided with the unhurried current plate 6 that dry thickness is 3mm, described stream on described supporting plate 4
Inlet port connector 7 is provided with body entrance 1, inlet port connector 7 is threadedly attached to the fixed seat 11 on flow damper housing 3, institute
The outside of fixed seat 11 stated is provided with reinforcement 12, is provided with outlet port connector 8 at described fluid issuing 2, on described flow damper
It is additionally provided with liquid level detector 14.The junction of described fixed seat 11 and flow damper housing 3 is provided with the collection pressure that two bores are 3mm
Mouthfuls 9, flow damper housing 3 it is corresponding with collection pressure mouth 9 be oblique angle for 60 degree of horn-like spout 10, described outlet port connector 8
The funnel-form outlet 13 that oblique angle is 60 degree is provided with the junction of flow damper housing 3.Described unhurried current plate 6 is cellular, thereon
It is some provided with the through hole that aperture is 1mm.
The course of work of the present utility model：The utility model proposes a kind of fluid unhurried current applied to cell culture chamber
Device, the flow damper are located between peristaltic pump and flow control valve, and the fluid that peristaltic pump pumps out is introduced at flow damper fluid intake
Fixed seat carry out collection pressure, be then ejected into from collection pressure mouth by horn-like spout on unhurried current plate, unhurried current plate be it is cellular, its
Effectively fluid flow and flow velocity it can enter row buffering and homogenize, and fluid in flow damper can conveniently be monitored by liquid level detector
Liquid level so that the fluid energy assured uniform of fluid issuing outflow, so that the rate of flow of fluid and flow that pass through flow control valve
Held stationary, greatly promote the reliability of fluid environment simulation.
Finally illustrate, preferred embodiment above is only unrestricted to illustrate the technical solution of the utility model, to the greatest extent
The utility model has been described in detail by above preferred embodiment for pipe, but those skilled in the art should manage
Solution, can make various changes, without departing from the utility model claims book institute to it in the form and details
The scope of restriction.
Claims (3)
- A kind of 1. fluid flow damper applied to cell culture chamber, it is characterised in that：It is 10mm's that the flow damper, which is provided with bore, The flow damper housing (3) of fluid intake (1), the fluid issuing (2) that bore is 6mm and PVC material, described flow damper housing (3) inner bay is provided with three layers of supporting plate (4), and through hole (5) is offered on supporting plate (4), described supporting plate (4) if on be provided with dry thickness For 3mm unhurried current plate (6), described fluid intake (1) place is provided with inlet port connector (7), and inlet port connector (7) is connected by screw thread The fixed seat (11) being connected on flow damper housing (3), described fixed seat (11) outside are provided with reinforcement (12), described fluid Outlet (2) place is provided with outlet port connector (8), and liquid level detector (14) is additionally provided with described flow damper.
- A kind of 2. fluid flow damper applied to cell culture chamber according to claim 1, it is characterised in that：Described The junction of fixed seat (11) and flow damper housing (3) is provided with the collection that two bores are 3mm and presses mouth (9), flow damper housing (3) with Collection pressure mouth (9) it is corresponding be that oblique angle is 60 degree of horn-like spout (10), described outlet port connector (8) and flow damper housing (3) junction is provided with the funnel-form that oblique angle is 60 degree and exports (13).
- A kind of 3. fluid flow damper applied to cell culture chamber according to claim 2, it is characterised in that：Described The plate (6) that flows slowly is cellular, and it is some to which is provided with the through hole that aperture is 1mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720630758.2U CN207210446U (en) | 2017-06-01 | 2017-06-01 | A kind of fluid flow damper applied to cell culture chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720630758.2U CN207210446U (en) | 2017-06-01 | 2017-06-01 | A kind of fluid flow damper applied to cell culture chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207210446U true CN207210446U (en) | 2018-04-10 |
Family
ID=61811310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720630758.2U Expired - Fee Related CN207210446U (en) | 2017-06-01 | 2017-06-01 | A kind of fluid flow damper applied to cell culture chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207210446U (en) |
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2017
- 2017-06-01 CN CN201720630758.2U patent/CN207210446U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180410 Termination date: 20190601 |