CN204455070U - A kind of cell fluid mechanics experimental apparatus - Google Patents
A kind of cell fluid mechanics experimental apparatus Download PDFInfo
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- CN204455070U CN204455070U CN201420709792.5U CN201420709792U CN204455070U CN 204455070 U CN204455070 U CN 204455070U CN 201420709792 U CN201420709792 U CN 201420709792U CN 204455070 U CN204455070 U CN 204455070U
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- silicone rubber
- rubber tube
- flow cavity
- storage tank
- liquid storage
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Abstract
A kind of cell fluid mechanics experimental apparatus, containing liquid storage tank, peristaltic pump, silicone rubber tube, T-shaped flow cavity, pressure-detecting device, flow control valve; This device connects in liquid storage tank by one end of silicone rubber tube, and the other end of silicone rubber tube is connected to the upper end entrance of T-shaped flow cavity through peristaltic pump; The left outlet of T-shaped flow cavity and right exit are provided with two tensimeters and are connected conducting through silicone rubber tube with liquid storage tank with flow control valve; Above-mentioned liquid storage tank, peristaltic pump, T-shaped flow cavity, tensimeter, flow control valve all connect and compose entirety by silicone rubber tube; When this peristaltic pump is started working, the nutrient solution in liquid storage tank is pumped, flow into T-shaped flow cavity through silicone rubber tube, just can form shock action; Surging force size is controlled, the T-shaped flow cavity internal pressure of gauge measurement by the rotating speed of peristaltic pump and the flow velocity of Flow-rate adjustment valve regulation nutrient solution.
Description
one, technical field
the utility model relates to a kind of cell fluid mechanics experimental apparatus, belongs to biomechanics field, for studying the reaction of cell to lateral fluid force of vitro culture.
two, background technology
Cell biomechanics is an important component part in field of tissue engineering technology, and cyto-mechanics experimental technique is then the basis of cyto-mechanics research.
Foreign scholar Michael P. Szymanski, Eleni Metaxa, Hui Meng, and John Kolega is at Ann Biomed Eng. 2008 October; 36 (10): 1681 – 1689. deliver the article being entitled as Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation, in literary composition, describe a kind of cell fluid mechanics experimental apparatus of T-shaped flow cavity.It is mainly used for studying the reaction of cell under hydromeehanics stimulates, as the change of endotheliocyte under different shearing stress effect in its form, motion and function etc. near simulation arteries crotch.This cell fluid mechanics device has some to be unfavorable for testing smoothly, the problem of research fast is further exactly: 1, in crowd the pressure value of Different Individual not etc., in its body, the fluid impact environment of generation is not different yet; And it is also different at the fluid impact environment at the vascular bifurcation place of same individual different sites; But in whole experimentation, it is 250mL/min and 500mL/min two shock environments that this device can only simulate flow velocity, in this and body, fluid impact environment is far apart really.2, whole device is in the process of impacting, and it is unknown whether the shock environment in flow cavity is stablized; This uncertain factor will produce tremendous influence to whole experimental result.3, this device simulates a hydromechanical environment by change flow (by increasing or reducing influx) and geometry (by increasing or reduce height or the width of inlet/outlet passage).If change a mechanical environment research, then need to adjust the structure of T-shaped flow cavity.This process not only makes complexity but also can increase workload and the cost of experiment.
three, summary of the invention
The purpose of this utility model provides a kind of cell fluid mechanics experimental apparatus, is characterized in that this device is conducive to experiment and carries out smoothly, fast, to ensure after T-shaped flow cavity homeostasis and reinforcing that cell continues to cultivate and pollution-free.
A kind of cell fluid mechanics experimental apparatus, containing liquid storage tank, peristaltic pump, silicone rubber tube, T-shaped flow cavity, pressure-detecting device, flow control valve; This device connects in liquid storage tank by one end of silicone rubber tube, and the other end of silicone rubber tube is connected to the upper end entrance of T-shaped flow cavity through peristaltic pump; The left outlet of T-shaped flow cavity and right exit are provided with two tensimeters and are connected conducting through silicone rubber tube with liquid storage tank with flow control valve; Above-mentioned liquid storage tank, peristaltic pump, T-shaped flow cavity, tensimeter, flow control valve all connect and compose entirety by silicone rubber tube; When this peristaltic pump is started working, the nutrient solution in liquid storage tank is pumped, flow into T-shaped flow cavity through silicone rubber tube, just can form shock action; Surging force size is controlled, gauge measurement T-shaped flow cavity internal pressure (its numerical value can be converted into corresponding value of shearing afterwards by Fluid Mechanics Computation analogue simulation) by the rotating speed of peristaltic pump and the flow velocity of Flow-rate adjustment valve regulation nutrient solution.
Described T-shaped flow cavity is the geometry block manufactured by polycarbonate material, and it has left side upper plate, right side upper plate, lower plate to form; The left outlet of described T-shaped flow cavity and right exit are provided with two described tensimeters with described flow control valve and are connected conducting with the described silicone rubber tube of described liquid storage tank; Described upper end entrance is communicated with described liquid storage tank by described silicone rubber tube equally; In described T-shaped flow cavity, have a T-shaped groove, described T-shaped groove surrounds into stream room, is provided with sealing groove, puts silicone rubber O-ring in sealing groove outside described T-shaped groove; Be provided with a slide glass groove in described lower plate, the cover glass of 22*50mm can be held; Three the geometry blocks forming described T-shaped flow cavity are respectively had eight bolt slots and are connected into the entirety of a described T-shaped flow cavity by stainless steel bolt, are provided with pad between stainless steel bolt and three geometry blocks.
The utility model tool has the following advantages:
1. aseptically process is simple, silicone rubber tube and silicone rubber O-ring are immersed in autoclave sterilization after in distilled water, because T-shaped flow cavity can use distilled water (dual anti-containing 100U/ml) to clean rear uviolizing 1 hour, autoclave sterilization after liquid storage tank is clean.Cell after such guarantee reinforcing continues to cultivate and pollution-free.
Can allow in flow cavity by regulating the rotating speed of the flow control valve outside T-shaped flow cavity and peristaltic pump and form different shock environments, operation is simple and feasible for this.The not environment of fluid impact at same area vascular bifurcation place or the fluid impact environment at the vascular bifurcation place of same individual different sites in consubstantiality can be simulated.Like this can not only be consistent with hydrodynamics environment real in body, and more fully can meet the needs of experiment, decrease workload and the cost of experiment.
In the process of whole impact, the tensimeter outside T-shaped flow cavity can shock environment in Real-Time Monitoring flow cavity.This not only plays a part very crucial to setting up a stable hydrodynamics environment but also whole experimental result can also be made to have more cogency.
Whole augmenter small volume, comprising peristaltic pump can put into cell culture incubator, and liquid storage tank opening communicates with air, ensure that the CO in substratum
2stable.
four, accompanying drawing explanation
Fig. 1 is cell fluid mechanics experimental apparatus schematic diagram.
1 liquid storage tank, 2 peristaltic pumps, 3 silicone rubber tubes, 4T type flow cavity, 5 tensimeters, 6 flow control valves
Fig. 2 is T-shaped flow cavity structural representation.
End entrance on 7, upper plate on the left of in the of 18, upper plate on the right side of in the of 8,19 left outlets, 9 right outlets, 10 stainless steel bolts, 11 pads, 12 sealing grooves, 13 silicone rubber O-rings, 14 stream rooms, 15 slide glass grooves, 16 lower plates, 17 bolt slots
five, specific embodiments
Below by embodiment, the utility model is specifically described, but the restriction to the utility model protection domain can not be interpreted as.
Embodiment
A kind of cell fluid mechanics experimental apparatus, as shown in Fig. 1 ~ 2, containing liquid storage tank 1, peristaltic pump 2, silicone rubber tube 3, T-shaped flow cavity 4, pressure-detecting device, flow control valve 6; This device connects in liquid storage tank 1 by one end of silicone rubber tube 3, and the other end of silicone rubber tube 3 is connected to the upper end entrance 7 of T-shaped flow cavity 4 through peristaltic pump 2; The left outlet 19 of T-shaped flow cavity 4 and right outlet 9 place are provided with two tensimeters 5 and are connected conducting through silicone rubber tube 3 with liquid storage tank 1 with flow control valve 6; Above-mentioned liquid storage tank 1, peristaltic pump 2, T-shaped flow cavity 4, tensimeter 5, flow control valve 6 all connect and compose entirety by silicone rubber tube 3.When this peristaltic pump 2 is started working, the nutrient solution in liquid storage tank 1 is pumped, flow into T-shaped flow cavity 4 through silicone rubber tube 3, just can form shock action.Regulated the flow velocity of nutrient solution by the rotating speed of peristaltic pump 2 and flow control valve 6 and controlled surging force size, T-shaped flow cavity 4 internal pressure (its numerical value can be converted into corresponding value of shearing afterwards by Fluid Mechanics Computation analogue simulation) measured by tensimeter 5.
Optimal way: as shown in Figure 2, described T-shaped flow cavity 4 is the geometry blocks manufactured by polycarbonate material, and it has left side upper plate 18, right side upper plate 8, lower plate 16 to form; The left outlet 19 of described T-shaped flow cavity 4 and right outlet 9 place are provided with two described tensimeters 5 with described flow control valve 6 and are connected conducting with the described silicone rubber tube 3 of described liquid storage tank 1; Described upper end entrance 7 is communicated with described liquid storage tank 1 by described silicone rubber tube 3 equally; In described T-shaped flow cavity 4, have a T-shaped groove, described T-shaped groove surrounds into stream room 14, is provided with sealing groove 12, puts silicone rubber O-ring 13 in sealing groove 12 outside described T-shaped groove; Be provided with a slide glass groove 15 in described lower plate 16, the cover glass of 22*50mm can be held; Three the geometry blocks forming described T-shaped flow cavity 4 are respectively had eight bolt slots 17 and are connected into the entirety of a described T-shaped flow cavity 4 by stainless steel bolt 10, are provided with pad 11 between stainless steel bolt 10 and three geometry blocks.
Claims (2)
1. a cell fluid mechanics experimental apparatus, containing liquid storage tank (1), peristaltic pump (2), silicone rubber tube (3), T-shaped flow cavity (4), pressure-detecting device, flow control valve (6); It is characterized in that: this device connects in liquid storage tank (1) by one end of silicone rubber tube (3), the other end of silicone rubber tube (3) is connected to the upper end entrance (7) of T-shaped flow cavity (4) through peristaltic pump (2); The left outlet (19) of T-shaped flow cavity (4) and right outlet (9) place are provided with two tensimeters (5) and are connected conducting through silicone rubber tube (3) with liquid storage tank (1) with flow control valve (6); Above-mentioned liquid storage tank (1), peristaltic pump (2), T-shaped flow cavity (4), tensimeter (5), flow control valve (6) all pass through silicone rubber tube (3) and connect and compose entirety.
2. a kind of cell fluid mechanics experimental apparatus as claimed in claim 1, is characterized in that: described T-shaped flow cavity (4) is the geometry block manufactured by polycarbonate material, and it has left side upper plate (18), right side upper plate (8), lower plate (16) to form; The left outlet (19) of described T-shaped flow cavity (4) and right outlet (9) place are provided with two described tensimeters (5) with described flow control valve (6) and are connected conducting with described liquid storage tank (1) described silicone rubber tube (3); Described upper end entrance (7) is communicated with described liquid storage tank (1) by described silicone rubber tube (3) equally; In described T-shaped flow cavity (4), there is a T-shaped groove, outside described T-shaped groove, be provided with sealing groove (12), in sealing groove (12), put silicone rubber O-ring (13); Be provided with a slide glass groove (15) in described lower plate (16), the cover glass of 22*50mm can be held; Three the geometry blocks forming described T-shaped flow cavity (4) are respectively had eight bolt slots (17) and are connected into the entirety of a described T-shaped flow cavity (4) by stainless steel bolt (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420709792.5U CN204455070U (en) | 2014-11-24 | 2014-11-24 | A kind of cell fluid mechanics experimental apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420709792.5U CN204455070U (en) | 2014-11-24 | 2014-11-24 | A kind of cell fluid mechanics experimental apparatus |
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| Publication Number | Publication Date |
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| CN204455070U true CN204455070U (en) | 2015-07-08 |
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| CN201420709792.5U Expired - Fee Related CN204455070U (en) | 2014-11-24 | 2014-11-24 | A kind of cell fluid mechanics experimental apparatus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154316A (en) * | 2015-09-30 | 2015-12-16 | 重庆大学 | Blood vessel-like running cavity |
| CN105277559A (en) * | 2015-09-28 | 2016-01-27 | 北京大学 | Microscopic imaging apparatus for pressure-adjustable flow field |
| CN105296326A (en) * | 2015-09-30 | 2016-02-03 | 重庆大学 | Vascular fluid platform with independent chamber |
-
2014
- 2014-11-24 CN CN201420709792.5U patent/CN204455070U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277559A (en) * | 2015-09-28 | 2016-01-27 | 北京大学 | Microscopic imaging apparatus for pressure-adjustable flow field |
| CN105277559B (en) * | 2015-09-28 | 2019-05-31 | 北京大学 | Microscopic imaging device and the device used with microscopes |
| CN105154316A (en) * | 2015-09-30 | 2015-12-16 | 重庆大学 | Blood vessel-like running cavity |
| CN105296326A (en) * | 2015-09-30 | 2016-02-03 | 重庆大学 | Vascular fluid platform with independent chamber |
| CN105154316B (en) * | 2015-09-30 | 2017-04-12 | 重庆大学 | Blood vessel-like running cavity |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150708 Termination date: 20151124 |