CN202440517U - Three-dimensional stress cell culture device - Google Patents
Three-dimensional stress cell culture device Download PDFInfo
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- CN202440517U CN202440517U CN 201220005555 CN201220005555U CN202440517U CN 202440517 U CN202440517 U CN 202440517U CN 201220005555 CN201220005555 CN 201220005555 CN 201220005555 U CN201220005555 U CN 201220005555U CN 202440517 U CN202440517 U CN 202440517U
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The utility model relates to a three-dimensional stress cell culture device. The three-dimensional stress cell culture device comprises a cardiac pump, a compliance part, a cell culture device, a back load, a liquid storage tank, a constant temperature device and the like, wherein the cardiac pump is used for generating a periodic pulsating flow, the output end is connected with the compliance part and then divided into two lines, one line is connected with the liquid storage tank and the constant temperature device through a branch and the back load of the branch, and the other line is connected with the liquid storage tank and the constant temperature device through the cell culture device. The three-dimensional stress cell culture device disclosed by the utility model can culture endothelial cells in the pulsating flow simulating a blood flow, simultaneously provide mechanical stimulation of circumferential stress, positive stress and tangential stress for the endothelial cells, relatively independently regulate the stress and provide a culture means for culturing the endothelial cells with tolerance to a hemodynamic factor.
Description
Technical field
The utility model relates to a kind of culture apparatus of vascular endothelial cell, especially a kind of simulation endotheliocyte in pulsating flow surrounding, and in hoop stress, shearing stress, the culture apparatus under the normal stress synergy.
Background technology
Vascular endothelial cell is the basis of the various physiological and pathological reactions of blood vessel, and utilizing culture endothelial cells is the important method of research endotheliocyte biology and various diseases relation.
The endotheliocyte of static cultivation method does not bear the effect of blood flow shearing stress, and the long more differentiation capability of isolated time is poor more, in subsequent experimental, is difficult to tolerate the haemodynamics environment and cell detachment takes place.Parallel flat flow chamber and strain chamber can be studied shearing stress and the blood vessel hoop stress influence to endothelial cell morphology and function of flowing respectively; But also there is not device can be provided at the normal stress that the body endotheliocyte receives, shearing stress and hoop stress simultaneously in blood vessel at present.
Summary of the invention
The utility model is that a kind of three dimensional stress cell culture apparatus will be provided; Endotheliocyte is cultivated in pulsating flow; This device can provide the hoop stress that endotheliocyte receives, shearing stress, normal stress simultaneously; And relatively independent adjusting, for culture endothelial cells provides laboratory facilities in order to research endothelial cell morphology function.
To achieve these goals, the utility model adopts technical scheme to be: a kind of three dimensional stress cell culture apparatus comprises heart pump; Compliance members, branch, branch's back loading; Cell culture apparatus, back loading, reservoir and thermostat; Simulated blood vessel is characterized in: cell culture apparatus inside comprises one section simulated blood vessel, the outside connection ratio of cell culture apparatus pressure regulation constant-current stabilizer; Heart pump produces and to divide two roads after periodically pulsing stream output terminal connects compliance members: leading up to branch is connected reservoir and thermostat with the branch back loading, and another road is passed through cell culture apparatus and is connected reservoir and thermostat with back loading.
Ratio pressure regulation constant-current stabilizer is by air, gas-holder, and proportional pressure valve, the gas tank pressure transmitter is formed, and the air air outlet connects gas-holder and proportional pressure valve, is connected with the gas tank pressure transmitter on the gas-holder.
Cell culture apparatus liquid flows into, outflow end is separately installed with the pressure transmitter near, far away that is used for measure analog blood vessel near-end and remote pressure, and is placed on measuring point near, far away respectively.
Be equipped with on the pipeline between back loading and reservoir and the thermostat and be used to measure the magnetic flow meter of liquid flow through the simulated blood vessel flow.
The simulated blood vessel that carries endotheliocyte in the cell culture apparatus is internal diameter 4～5 mm, the silicone tube of length 80 mm.
The beneficial effect of the utility model is:
1. vascular endothelial cell is cultivated under pulsating flow;
2. the normal stress that receives simultaneously of endotheliocyte, shearing stress, the effect of hoop stress, these power can relatively independently be regulated.
Therefore; The utility model is cultivated endotheliocyte in pulsating flow; Simultaneously can simulate hoop stress, shearing stress, the normal stress that endotheliocyte receives, and can be relatively independently regulated, for culture endothelial cells provides laboratory facilities in order to study its morphological function.
Description of drawings
Fig. 1 is the structured flowchart of the utility model;
Fig. 2 is the cell culture apparatus structural representation of the utility model.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described.
As shown in Figure 1, the three dimensional stress cell culture apparatus of the utility model comprises heart pump 1, compliance members 2, branch 3, branch's back loading 4, cell culture apparatus 5 etc.Heart pump 1 produces periodically pulsing stream output terminal and connects compliance members 2 backs and divide two roads: leading up to branch 3 is connected reservoir and thermostat 15 with branch back loading 4, and another road is passed through cell culture apparatus 5 and is connected reservoir and thermostat 15 with back loading 13.
As shown in Figure 2, cell culture apparatus 5 inside comprise one section simulated blood vessel 6.Simulated blood vessel 6 outside connection ratio pressure regulation constant-current stabilizers 16.Simulated blood vessel 6 liquid flow into, outflow end is separately installed with the pressure transmitter 11,12 near, far away that is used for measure analog blood vessel 6 near-ends and remote pressure, and are placed on measuring point near, far away respectively.Simulated blood vessel 6 connects reservoir and thermostat 15 through back loading 13.Between back loading 13 and reservoir and the thermostat 15 magnetic flow meter 14 is housed.
Ratio pressure regulation constant-current stabilizer 16 comprises air 7, gas-holder 8, proportional pressure valve 9, gas tank pressure transmitter 10.Air 7 air outlets connect gas-holder 8 and proportional pressure valve 9, are connected with gas tank pressure transmitter 10 on the gas-holder 8.
Heart pump 1 can periodically be penetrated blood by anthropomorphic dummy's systemic heart; Compliance members 2 simulation arterial elasticity chamber functions are penetrated the intermittence of pulsation core pump 1 at blood and are become the successive pulsating flow, and branch 3 is used to regulate the liquid flow rate size of another branch road; Branch's back loading 4 and the Peripheral resistance of back loading 13 simulation human capillary vessels to artery; Cell culture apparatus 5 inside have comprised one section simulated blood vessel 6, and the outside of cell culture apparatus 5 is connecting air 7 and is being cell culture apparatus 5 air feed, gas-holder 8 steady air flows; Proportional pressure valve 9 control cell culture apparatuses 5 internal pressures, pressure transmitter 10 is measured gas-holder 8 internal pressures.Pressure transmitter 11,12 is placed in the liquid inflow and outflow end of cell culture apparatus 5 respectively, and magnetic flow meter 14 is measured the flow of liquid flow through simulated blood vessel, and reservoir and thermostat 15 provide constant temp for flowing fluid.
The simulated blood vessel 6 that the cell culture apparatus of three dimensional stress cell culture apparatus carries endotheliocyte is internal diameter 4～5 mm, the silicone tube of length 80 mm.Closely, measuring point far away is placed pressure transmitter 11,12 near, far away, measure analog blood vessel 6 near-ends and remote pressure.
Be placed in the cell culture apparatus 5 behind the plantation endotheliocyte in the simulated blood vessel 6; Heart pump 1 produces periodically pulsing stream, and liquid divides two branch roads, flow through a branch 3 and a back loading 4; Another flow through cell culture apparatus 5 and back loading 13, last branch road plays the effect of shunting.When the liquid increase of the branch 3 that flows through, the liquid flow rate of the simulated blood vessel 6 of flowing through reduces, and endotheliocyte receives shearing stress and reduces.
Air 7 inflations, gas is through gas-holder 8 voltage stabilizings, and gas-holder 8 top placement force transmitters 10 are simulated blood vessel 6 outer wall pressure in order to measure a jar internal pressure; Gas stream is through proportional pressure valve 9; The final cell culture apparatus 5 that gets into changes simulated blood vessel 6 outer wall pressure, thereby regulates the hoop stress that endotheliocyte receives; Outer wall pressure is big more, and hoop stress is more little.When gas-holder 8 internal pressures arrive 30 kpa, air 7 stops air feed; When cell culture apparatus 5 internal gas pressures less than preset value, the gas passing ratio pressure valve 9 of storage in the gas-holder 8 continue to its air feed; When cell culture apparatus 5 internal gas pressures greater than preset value, gas-holder 8 stops air feed, proportional pressure valve 9 is opened and is discharged gases to realize decompression.
Closely, pressure transmitter 11,12 far away respectively the pressure of measure analog blood vessel 6 inflow and outflow ends be the normal stress of near-end and far-end, this observed value can also calculate the hoop stress that endotheliocyte receives respectively at simulated blood vessel 6 near-ends and far-end.Thereby magnetic flow meter 14 is measured the liquid flow rate of the simulated blood vessel 6 of flowing through and is calculated shearing stress.Increase back loading 13 and promptly increase Peripheral resistance, thereby increase the normal stress that endotheliocyte receives.
Claims (5)
1. a three dimensional stress cell culture apparatus comprises heart pump (1), compliance members (2); Branch (3), branch's back loading (4), cell culture apparatus (5); Simulated blood vessel (6); Back loading (13), reservoir and thermostat (15) is characterized in that: said cell culture apparatus (5) inside comprises one section simulated blood vessel (6); Cell culture apparatus (5) outside connection ratio pressure regulation constant-current stabilizer (16); Said heart pump (1) produces periodically pulsing stream output terminal and connects compliance members (2) back and divide two roads: leading up to branch (3) is connected reservoir and thermostat (15) with branch's back loading (4), another road is passed through cell culture apparatus (5) and is connected reservoir and thermostat (15) with back loading (13).
2. three dimensional stress cell culture apparatus according to claim 1; It is characterized in that: said ratio pressure regulation constant-current stabilizer (16) is by air (7); Gas-holder (8), proportional pressure valve (9), gas tank pressure transmitter (10) is formed; Air (7) air outlet connects gas-holder (8) and proportional pressure valve (9), is connected with gas tank pressure transmitter (10) on the gas-holder (8).
3. three dimensional stress cell culture apparatus according to claim 1; It is characterized in that: said cell culture apparatus (5) liquid flows into, outflow end is separately installed with the pressure transmitter (11,12) near, far away that is used for measure analog blood vessel (6) near-end and remote pressure, and is placed on measuring point near, far away respectively.
4. three dimensional stress cell culture apparatus according to claim 1 is characterized in that: be equipped with on the pipeline between said back loading (13) and reservoir and the thermostat (15) and be used to measure liquid flow through simulated blood vessel flow magnetic flow meter (14).
5. three dimensional stress cell culture apparatus according to claim 1 is characterized in that: said simulated blood vessel (6) is internal diameter 4～5 mm, the silicone tube of length 80 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220005555 CN202440517U (en) | 2012-01-09 | 2012-01-09 | Three-dimensional stress cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220005555 CN202440517U (en) | 2012-01-09 | 2012-01-09 | Three-dimensional stress cell culture device |
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| CN202440517U true CN202440517U (en) | 2012-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220005555 Expired - Fee Related CN202440517U (en) | 2012-01-09 | 2012-01-09 | Three-dimensional stress cell culture device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952307A (en) * | 2014-04-29 | 2014-07-30 | 中国人民解放军第四军医大学 | In-vitro stress culture device for blood vessel, culture system and culture method |
| CN107974406A (en) * | 2017-12-01 | 2018-05-01 | 西安交通大学 | Intravascular stent is degraded and fatigue property test bioreactor and its test method |
| CN108130274A (en) * | 2017-08-18 | 2018-06-08 | 上海健康医学院 | A kind of cell culture apparatus and its method under pressure and single direction strain correlation |
-
2012
- 2012-01-09 CN CN 201220005555 patent/CN202440517U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103952307A (en) * | 2014-04-29 | 2014-07-30 | 中国人民解放军第四军医大学 | In-vitro stress culture device for blood vessel, culture system and culture method |
| CN103952307B (en) * | 2014-04-29 | 2016-01-20 | 中国人民解放军第四军医大学 | A kind of vascular bodies external stress culture apparatus and culture systems and cultural method |
| CN108130274A (en) * | 2017-08-18 | 2018-06-08 | 上海健康医学院 | A kind of cell culture apparatus and its method under pressure and single direction strain correlation |
| CN108130274B (en) * | 2017-08-18 | 2024-01-23 | 上海健康医学院 | Cell culture device and method under association effect of pressure and unidirectional strain |
| CN107974406A (en) * | 2017-12-01 | 2018-05-01 | 西安交通大学 | Intravascular stent is degraded and fatigue property test bioreactor and its test method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120919 Termination date: 20130109 |
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| CF01 | Termination of patent right due to non-payment of annual fee |