CN107897169B - Isolated organ oxygenation perfusion device - Google Patents
Isolated organ oxygenation perfusion device Download PDFInfo
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- CN107897169B CN107897169B CN201711072699.2A CN201711072699A CN107897169B CN 107897169 B CN107897169 B CN 107897169B CN 201711072699 A CN201711072699 A CN 201711072699A CN 107897169 B CN107897169 B CN 107897169B
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- water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
Abstract
The invention discloses an isolated organ oxygenation perfusion device, which belongs to the field of isolated organ culture equipment and comprises a first water bathtub, wherein a container and an oxygenator are arranged in the first water bathtub, the oxygenator comprises a closed shell, a gas exchange tube is arranged in the shell, a gas input port is arranged at the lower end of the shell, a gas output port is arranged at the upper end of the shell, the lower end of the container is communicated with the lower end of the shell through a first connecting tube, the upper end of the shell is communicated with a second water bath cylinder through a second connecting tube, the shell comprises a gas exchange tube, the first connecting tube and the second connecting tube are communicated through the gas exchange tube, the container is provided with a pressurizing device, the gas exchange tube is silica gel, the wall thickness of the gas exchange tube is not more than 0.3mm, the inner diameter of the gas exchange tube is not less than 1mm, and the pressurizing device is of a reciprocating volume. The invention has simple and reasonable design, can ensure the effective gas exchange of the perfusate in the container, and has the function of not damaging the perfusate in the container.
Description
Technical Field
The invention relates to the field of isolated organ culture equipment, in particular to an isolated organ oxygenation perfusion device.
Background
The culture of the isolated organ requires that the isolated organ is perfused with a perfusate, and a certain amount of oxygen is contained in the perfusate; in the prior art, a bubbling type or membrane type oxygenator is adopted to add oxygen to the perfusate, but the bubbling type oxygenator can cause the perfusate to generate tiny bubbles, has the risk of forming air embolism, and cannot be used for oxygenation of the perfusate containing components such as protein, fatty acid and the like; while membrane oxygenator devices are complex and expensive; in addition, in the prior art, the perfusion liquid channel is single, and when the perfusion of different perfusion liquids needs to be carried out in stages, a plurality of devices are often needed, so that the operation is complicated, the efficiency is low, the burden of workers is increased, and the error rate of operation is increased.
Disclosure of Invention
The invention aims to provide an isolated organ oxygenation perfusion device which is used for solving the problems that micro bubbles are generated by the existing oxygenation perfusion device to form air bolts, and the membrane type oxygenator is complex in structure and high in cost.
In order to achieve the above purpose, the invention adopts the following technical scheme: the utility model provides an isolated organ oxygenation perfusion device, includes first water bathtub, be equipped with container, oxygenator in the first water bathtub, oxygenator includes confined casing, be equipped with the gas exchange pipe in the casing, the casing lower extreme is equipped with gas input port, and the casing upper end is equipped with gas output port, the lower extreme of container communicates with the casing lower extreme through first connecting pipe, and the casing upper end communicates with the second water bath jar through the second connecting pipe, including the gas exchange pipe in the casing, first connecting pipe, second connecting pipe pass through the gas exchange pipe intercommunication, the container is equipped with pressurizing device.
Preferably, the lower extreme of casing is equipped with liquid input interface, and liquid input interface is equipped with liquid output interface through first connecting pipe and container intercommunication, and the casing upper end, liquid output port is through second connecting pipe and second water bath jar intercommunication, liquid input interface is connected to the lower extreme of gas exchange tube, and liquid output interface is connected to the upper end of gas exchange tube.
Preferably, the gas exchange tube is silica gel, the wall thickness of the gas exchange tube is not more than 0.3mm, and the inner diameter of the gas exchange tube is not less than 1mm.
Preferably, the second connecting pipe is provided with an exhaust valve and a flow sensor.
Preferably, the second water bath is of a double-layer structure, the second water bath comprises an inner layer and an outer layer, an interlayer is arranged between the inner layer and the outer layer, the interlayer is communicated with the first water bath tank, and the second connecting pipe is communicated with the inner layer of the second water bath.
Preferably, the inner layer of the second water bathtub is connected with a recovery hydraulic cylinder, the second water bathtub is communicated with the upper part of the recovery hydraulic cylinder, a valve is arranged between the second water bathtub and the recovery hydraulic cylinder, and the valve is a manual adjusting valve.
Further, a measuring cylinder is further arranged between the valve and the second water bath cylinder, the upper portion of the measuring cylinder is communicated with the inner layer of the second water bath cylinder, and the lower portion of the measuring cylinder is communicated with the recycling liquid cylinder through the valve.
Further, the recovery hydraulic cylinder is communicated with the container, and the recovery hydraulic cylinder is provided with a pressurizing device.
Preferably, the pressurizing device is a pump.
Preferably, the pump is a reciprocating volumetric pump.
Further, the pressurizing device is a piston pump, and the piston pumps are arranged on the first connecting pipe and the third connecting pipe;
of course, the pressurizing device may be a diaphragm pump, and the diaphragm pump is disposed on the first connecting pipe and the third connecting pipe.
Preferably, the pressurizing device is a piston metering pump or a diaphragm metering pump.
Preferably, a pressure monitoring device is arranged on the shell of the oxygenator, and the gas output port is connected with the pressure monitoring device.
Further, the pressure monitoring device is a pressure gauge.
Further, the pressure monitoring device is a water tank, and the free end of the gas output port extends into the lower part of the water surface of the water tank.
Preferably, the number of the containers, the first connecting pipes and the gas exchange pipes is at least two, and the number of the containers, the number of the first connecting pipes and the number of the gas exchange pipes are the same.
Preferably, a filter is arranged on the first connecting pipe.
The beneficial effects of the invention are as follows:
1. the gas exchange tube made of silica gel has the functions of water resistance and ventilation, avoids the damage of bubbles to components of the perfusate in the process of gas exchange of the perfusate, and has the advantage of low price compared with a gas exchange membrane in a membrane type oxygenator;
2. the arrangement of the perfusion liquid inside and the gas outside the gas exchange tube has the effect of enabling the gas exchange to be more sufficient;
3. the pressurizing device is a reciprocating displacement pump, so that the perfusate in the container is prevented from being damaged by the mechanical rotary element in the pressurizing process;
4. the third connecting pipe and the pressurizing device connected with the recovery hydraulic cylinder can be used for circulating operation when single perfusate is used, so that the amount of the required perfusate can be reduced, the preparation cost of the perfusate is saved, and the possibility is provided for simulating a closed circulation perfusion mode under physiological conditions;
5. the device has the capability of using various perfusates, can reduce the use cost, and can realize the switching and the perfusion of various perfusates or the combined perfusion of different proportions.
Drawings
FIG. 1 is a schematic diagram of a structure of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a second embodiment of the present invention;
FIG. 3 is a schematic diagram of a third embodiment of the present invention;
fig. 4 is a schematic structural diagram of a fourth embodiment of the present invention.
In the figure: 1-first water bathtub, 2-container, 3-oxygenator, 4-gas exchange pipe, 5-first connecting pipe, 6-gas input port, 7-gas output port, 8-second connecting pipe, 9-discharge valve, 10-second water bathtub, 11-recovery hydraulic cylinder, 12-pressurizing device, 13-flow sensor, 14-valve, 15-third connecting pipe, 16-measuring cylinder.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the present invention more apparent.
Embodiment one:
as shown in fig. 1, an isolated organ oxygenation perfusion apparatus comprises a first water bathtub 1, wherein a container 2 and an oxygenator 3 are arranged in the first water bathtub 1, the oxygenator 3 comprises a shell, a gas exchange tube 4 is arranged in the shell, a gas input port 6 is arranged at the bottom of the shell, one end of the gas input port 6 is communicated with the bottom of the shell, the other end of the gas input port 6 penetrates through the bottom of the shell and the bottom of the first water bathtub 1, and a gas output port 7 is arranged at the top of the shell; the lower end of the shell is provided with a liquid input interface which is communicated with the container 2 through a first connecting pipe 5, the upper end of the shell is provided with a liquid output interface which is communicated with a second water bathtub 10 through a second connecting pipe 8;
the lower end of the gas exchange tube 4 is connected with a liquid input interface, and the upper end of the gas exchange tube 4 is connected with a liquid output interface; one end of the first connecting pipe 5 passes through the bottom of the first water bathtub 1 and the bottom of the container 2 to be communicated with the container 2, and the other end of the first connecting pipe 5 passes through the bottom of the first water bathtub 1 and the bottom of the shell to be communicated with the liquid input interface; one end of the second connecting pipe 8 penetrates through the side wall of the first water bathtub 1 and the side wall of the upper part of the shell to be communicated with the liquid output interface, and the container 2 is provided with a pressurizing device 12.
The gas exchange tube 4 is a silicone tube with the wall thickness not exceeding 0.3mm and the inner diameter not less than 1 mm;
the second connecting pipe 8 is provided with an exhaust valve 9 and a flow sensor 13, the exhaust valve 9 is communicated with the second connecting pipe 8 through a branch pipe, and the exhaust valve 9 is used for releasing gas in the second connecting pipe 8 and preventing the gas from entering tissues to form a gas plug;
of course, the second connecting pipe 8 may be connected with a ball valve or a manual adjusting valve for exhausting.
The first connecting pipe 5 is provided with a filter which can filter out dregs in the perfusate.
The second water bathtub 10 is of a double-layer structure, the second water bathtub 10 comprises an inner layer and an outer layer, an interlayer is arranged between the inner layer and the outer layer, the interlayer is communicated with the first water bathtub 1, the second connecting pipe 8 is communicated with the inner layer of the second water bathtub 10, and the second connecting pipe 8 is communicated with the upper part of the second water bathtub 10;
the inner layer of the second water bathtub 10 is connected with a recovery hydraulic cylinder 11, the second water bathtub 10 is positioned above the recovery hydraulic cylinder 11, the upper part of the second water bathtub 10 is connected with the top of the recovery hydraulic cylinder 11 through a valve 14, and the valve 14 is a manual regulating valve; a measuring cylinder 16 is arranged between the second water bathtub 10 and the valve 14, the upper part of the measuring cylinder 16 is communicated with the inner layer of the second water bathtub 10, and the lower part of the measuring cylinder 16 is communicated with the top of the recovery hydraulic cylinder 14 through the valve 14;
when the valve 14 is closed, the injection speed of the perfusate in the second water bath tank 10 can be obtained by calculating the volume increase of the perfusate in the measuring cylinder 16 within a certain time; when valve 14 is opened, the perfusate flows into recovery cylinder 11.
A pressure monitoring device is arranged on the shell of the oxygenator 3, and a gas output port 7 is connected with the pressure monitoring device;
the pressure monitoring device is a water tank, and the free end of the gas output port 7 extends below the water surface of the water tank; whether gas is present in the oxygenator 3 is determined by observing bubbles under the water surface in the water tank.
The pressurizing device 12 is a pump, and the pump is a reciprocating displacement pump, and the reciprocating displacement pump moves left and right in the pump body through the movable element so as to generate circulating positive pressure and negative pressure in the pump body to convey liquid, thereby having the effect of small damage to perfusate.
Specifically, the pressurizing device 12 is a piston pump, and meanwhile, in order to calculate and adjust the usage amount of the perfusate, the pressurizing device 12 is a piston type metering pump, the piston type metering pump is installed on the first connecting pipe 5 and is used for pumping the perfusate of the container 2 into the second water bathtub 10, and the piston type metering pump generates circulating negative pressure and positive pressure to convey the liquid through the left and right movement of the piston, so that culture substances in the perfusate cannot be damaged due to the rotation of a machine;
or the pressurizing device 12 is a diaphragm pump, and meanwhile, in order to calculate and adjust the usage amount of the perfusate, the pressurizing device 12 is a diaphragm type metering pump which is arranged on the first connecting pipe 5 and is used for pumping the perfusate of the container 2 into the second water bathtub 10, and meanwhile, the diaphragm type metering pump can pump in and out liquid through a diaphragm, so that culture substances in the perfusate cannot be damaged due to the rotation of a mechanical element;
of course, the pressurizing means 12 may also be a peristaltic pump.
When the novel oxygen generator is used, the first water bathtub 1 is started to enable the water temperature of the first water bathtub 1 and the water temperature of the second water bathtub communicated with the first water bathtub 1 to reach the experimental temperature, meanwhile, oxygen is introduced into the gas input port 6, and after bubbles in a water tank on the shell of the oxygen generator 3 emerge, a period of time is waited for to enable the oxygen generator 3 to be filled with oxygen; starting the pressurizing device 12 again, and enabling the perfusate contained in the container 2 to enter the gas exchange tube 4 through the first connecting tube 5 under the action of the pressurizing device 12, wherein the perfusate in the gas exchange tube 4 flows while oxygen in the shell enters the gas exchange tube 4 to complete gas exchange due to the waterproof air permeability of the silica gel thin-wall tube, so that the oxygen content in the perfusate is increased; the exhaust valve 9 is opened again to exhaust the air in the second connection pipe 8, and the perfusate finally flows to the second bathtub 10 to perfuse the tissue in the second bathtub 10.
Embodiment two:
as shown in fig. 2, it is different from the first embodiment in that: the recovery hydraulic cylinder 11 is communicated with the container 2, the recovery hydraulic cylinder 11 is provided with a pressurizing device 12, and the pressurizing device 12 is used for sending the perfusion liquid in the recovery hydraulic cylinder 11 back into the container 2 for recycling; the recovery hydraulic cylinder 11 and the container 2 are respectively connected with a pressurizing device 12, and the two pressurizing devices 12 are respectively arranged on the first connecting pipe 5 and the third connecting pipe 15.
In use, the perfusate flowing out of the container 2 continuously flows into the second water bathtub 10 after being oxygenated, but the capacity of the second water bathtub 10 is limited, meanwhile, in order to reduce the cost, the used perfusate is re-sent into the container 2 by the pressurizing device 12 after flowing out of the second water bathtub 10 into the recycling liquid cylinder 11, and the preheating and oxygenation processes are performed again, so that the purpose of recycling is achieved, the total use amount of the perfusate can be saved, and the cost is reduced.
Embodiment III:
as shown in fig. 3, it is different from the first embodiment in that: the container 2 and the first connecting pipes 5 are all provided with three mutually independent gas exchange pipes 4, three liquid input interfaces are arranged at the lower end of the shell, one liquid output interface is arranged at the upper end of the shell, the three containers 2 are respectively communicated with the three gas exchange pipes 4 through the three first connecting pipes 5, the three gas exchange pipes 4 are respectively communicated with the second water bathtub 10 through the second connecting pipes 8, and the pressurizing devices 12 are respectively arranged on the three first connecting pipes 5.
In use, when different types of perfusate are needed to be used, and simultaneously the different types of perfusate cannot be simultaneously perfused to an isolated organ, the device shown in fig. 3 can be used for independently using various perfusates without affecting each other. When a certain type of perfusate is needed, the pressurizing device 12 connected with the container for containing the perfusate is opened; meanwhile, the device shown in fig. 3 also avoids the defects of high use cost, complex operation and easy error caused by the need of using multiple sets of devices in the prior art.
Embodiment four:
as shown in fig. 4, it differs from the implementation three in that: the three containers 2 are all connected with a pressurizing device 12, the pressurizing device 12 is a multi-channel pump, the three first connecting pipes 5 are respectively connected with the multi-channel pump, and specifically, the pressurizing device 12 is a three-channel peristaltic pump.
In use, the perfusate in the three containers 2 is connected to the three-way peristaltic pump through three first connecting pipes 5, respectively; this embodiment can reduce the number of pressurizing devices 12 and save costs when three perfusion solutions are to be simultaneously perfused.
Of course, the present invention is capable of other various embodiments and its several details are capable of modification and variation in light of the present invention by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. An isolated organ oxygenation perfusion apparatus, characterized in that: the novel water bath comprises a first water bath body (1), wherein a container (2) and an oxygenator (3) are arranged in the first water bath body (1), the oxygenator (3) comprises a closed shell, a gas input port (6) is arranged at the lower end of the shell, a gas output port (7) is arranged at the upper end of the shell, the lower end of the container (2) is communicated with the lower end of the shell through a first connecting pipe (5), the upper end of the shell is communicated with a second water bath body (10) through a second connecting pipe (8), a gas exchange pipe (4) is arranged in the shell, the first connecting pipe (5) and the second connecting pipe (8) are communicated through the gas exchange pipe (4), and a pressurizing device (12) is arranged on the container (2);
the second water bathtub (10) is of a double-layer structure, the second water bathtub (10) comprises an inner layer and an outer layer, an interlayer is arranged between the inner layer and the outer layer, the interlayer is communicated with the first water bathtub (1), the second connecting pipe (8) is communicated with the inner layer of the second water bathtub (10), and the second connecting pipe (8) is communicated with the upper part of the second water bathtub (10);
the inner layer of the second water bathtub (10) is connected with a recovery hydraulic cylinder (11), the second water bathtub (10) is positioned above the recovery hydraulic cylinder (11), the upper part of the second water bathtub (10) is connected with the top of the recovery hydraulic cylinder (11) through a valve (14), and the valve (14) is a manual regulating valve; a measuring cylinder (16) is arranged between the second water bathtub (10) and the valve (14), the upper part of the measuring cylinder (16) is communicated with the inner layer of the second water bathtub (10), and the lower part of the measuring cylinder (16) is communicated with the top of the recycling hydraulic cylinder (14) through the valve (14).
2. The ex vivo organ oxygenation perfusion apparatus of claim 1, wherein: the gas exchange tube (4) is silica gel, the wall thickness of the gas exchange tube (4) is not more than 0.3mm, and the inner diameter of the gas exchange tube (4) is not less than 1mm.
3. The ex vivo organ oxygenation perfusion apparatus of claim 1, wherein: an exhaust valve (9) and a flow sensor (13) are arranged on the second connecting pipe (8).
4. The ex vivo organ oxygenation perfusion apparatus of claim 1, wherein: the second water bathtub (10) is connected with a recovery hydraulic cylinder (11), and a valve (14) is arranged between the second water bathtub (10) and the recovery hydraulic cylinder (11).
5. The ex vivo organ oxygenation perfusion apparatus of claim 4, wherein: the recovery hydraulic cylinder (11) is communicated with the container (2) through a third connecting pipe (15), and the recovery hydraulic cylinder (11) is provided with a pressurizing device (12).
6. The ex vivo organ oxygenation perfusion apparatus of claim 1 or 5, wherein: the pressurizing device (12) is a reciprocating displacement pump.
7. The ex vivo organ oxygenation perfusion apparatus of claim 1, wherein: the shell of the oxygenator (3) is provided with a pressure monitoring device, a gas output port (7) is connected with the pressure monitoring device, and the pressure monitoring device is a pressure gauge.
8. The ex vivo organ oxygenation perfusion apparatus of claim 7, wherein: the pressure monitoring device is a water tank, and the free end of the gas output port (7) stretches into the lower part of the water surface of the water tank.
9. The ex vivo organ oxygenation perfusion apparatus of claim 1, wherein: the lower extreme of casing is equipped with liquid input interface, and liquid input interface is equipped with liquid output interface through first connecting pipe (5) and container (2) intercommunication, and the casing upper end is equipped with liquid output interface, and liquid output port is through second connecting pipe (8) and second bathtub (10) intercommunication, liquid input interface is connected to the lower extreme of gas exchange tube (4), and liquid output interface is connected to the upper end of gas exchange tube (4).
10. The ex vivo organ oxygenation perfusion apparatus of any of claims 1-4, wherein: the number of the containers (2), the first connecting pipes (5) and the gas exchange pipes (4) is the same.
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| CN201711072699.2A CN107897169B (en) | 2017-11-03 | 2017-11-03 | Isolated organ oxygenation perfusion device |
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| CN201711072699.2A CN107897169B (en) | 2017-11-03 | 2017-11-03 | Isolated organ oxygenation perfusion device |
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| CN107897169B true CN107897169B (en) | 2023-09-15 |
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| CN111657268A (en) * | 2020-03-31 | 2020-09-15 | 同济大学 | Small animal heart imaging perfusion hose system |
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