CN214758844U - Separated small animal isolated organ perfusion container and assembly - Google Patents
Separated small animal isolated organ perfusion container and assembly Download PDFInfo
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- CN214758844U CN214758844U CN202120281682.3U CN202120281682U CN214758844U CN 214758844 U CN214758844 U CN 214758844U CN 202120281682 U CN202120281682 U CN 202120281682U CN 214758844 U CN214758844 U CN 214758844U
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Abstract
The utility model provides a disconnect-type toy isolated organ perfusion container and subassembly, its characterized in that: comprises a perfusion container body and a bypass perfusion structure; wherein the filling container body is in a cup shape with an opening part; the opening part is detachably provided with an object stage; the bypass perfusion structure comprises a bypass perfusion liquid inlet and a bypass perfusion liquid outlet; the bypass perfusate inlet and the bypass perfusate outlet are both communicated with the inner wall of the container of the perfusion container body. The perfusion container can improve the perfusion efficiency.
Description
Technical Field
The utility model relates to an experimental facilities specifically, relates to a disconnect-type toy isolated organ perfusion container and subassembly.
Background
In medical research, perfusion technology is widely applied, and experimental animals can obtain good morphological observation results and better preserve brain, kidney, heart and other organs through perfusion. Researchers generally adopt a cardiac puncture method for perfusion, tissues obtained through perfusion are prepared into paraffin sections, frozen sections and the like, and the method is further applied to the technical field of experiments such as immunohistochemistry. A large number of researches prove that the quality of the perfusion result has great influence on the experimental result.
The perfusion device often has a great influence on the experimental efficiency and the result. The existing perfusion device often has the problem that the experimental result is poor due to poor heat preservation effect on tissues/organs of an experiment in a perfusion stage.
And at present, perfusion equipment for small animals is not available, and perfusion is usually performed by adopting large organ perfusion equipment in the existing perfusion experiment process, but the equipment has huge size and complex system, the cost of each use is not less than 2 thousands, and when a complete experiment process needs to be completed, a large amount of money and energy consumption are caused.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the above-mentioned defect, provide an integrated, simple and easy toy trachea perfusion device.
The utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that: comprises a perfusion container body and a bypass perfusion structure;
wherein the filling container body is in a cup shape with an opening part;
the opening part is detachably provided with an objective table;
the bypass perfusion structure comprises a bypass perfusion liquid inlet and a bypass perfusion liquid outlet;
the bypass perfusate inlet and the bypass perfusate outlet are both communicated with the inner wall of the container of the perfusion container body.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the bypass perfusate inlet is arranged above the bypass perfusate outlet.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the bypass perfusate inlet is communicated with the bypass perfusate outlet through a pipeline;
the pipeline is also connected with a purification mechanism or a multi-way mechanism.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the pouring container body comprises a pouring container inner frame body and a pouring container outer frame body;
an insulating layer is arranged between the inner frame body and the outer frame body of the perfusion container.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the device also comprises a heat preservation liquid inlet and a heat preservation liquid outlet;
the heat preservation liquid inlet and the heat preservation liquid outlet are communicated with the outer frame body of the pouring container and communicated with the heat preservation layer.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the pouring container body is also provided with a pouring liquid output structure;
the perfusate output structure is communicated with the inner wall of the container of the perfusion container body.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the perfusate output structure is also connected with a purification mechanism.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the opening part of the pouring container body is provided with a positioning structure;
the positioning structure is matched with the bottom of the object stage.
Further, the utility model provides a pair of disconnect-type toy isolated organ perfusion container, its characterized in that still lies in: the bypass perfusate inlet is provided with a valve;
and/or
The bypass perfusate outlet is provided with a valve;
and/or
The perfusate output structure is provided with a valve.
In addition, the invention also provides a separated small animal isolated organ perfusion assembly, which is characterized in that: comprises the separated small animal isolated organ perfusion container, the object stage and/or the purification mechanism.
Utility model's effect and effect:
the utility model discloses an equipment size is less, and through the bypass structure, has realized purifying and the effect of renewal the perfusate. In addition, be different from traditional integral type filling device, the utility model discloses a filling device be the disconnect-type structure, promptly, filling container and objective table are separable structure, and such structure is in the in-process of using, can be through moving objective table (generally for the funnel structure) to observing the velocity of flow of its perfusate in equipment such as graduated flask.
Drawings
FIG. 1 is a schematic structural diagram of a perfusion container for isolated small animal isolated organs provided by an embodiment;
FIG. 2 is a schematic structural diagram of a perfusion container for isolated small animal isolated organs provided by an embodiment;
FIG. 3 is a schematic structural diagram of a perfusion container for isolated small animal isolated organs provided by an embodiment;
fig. 4 is a schematic structural diagram of the object stage according to the embodiment.
Detailed Description
As shown in fig. 1-3, the present embodiment provides a separate small animal isolated organ perfusion container 100, which includes a perfusion container body and a bypass perfusion structure;
the filling container body is in a cup shape with an opening part;
in order to realize the effect of carrying out specified temperature heat preservation on the pouring environment in the pouring container body, the pouring container body is provided with a heat preservation structure;
the specific form of the heat insulation structure can be as follows:
the perfusion container body consists of a perfusion container inner frame body 120 and a perfusion container outer frame body 110;
an insulating layer is arranged between the inner frame body and the outer frame body of the pouring container;
this mode that heat preservation was realized to heat preservation can be for burying mode such as heater strip underground in the heat preservation and realizing the bulk heating of heat preservation, also can be for the structure as shown in the figure:
namely, the upper part of the filling container body is provided with a heat preservation liquid outlet 111 which can run through the outer frame body of the filling container;
the lower part of the perfusion container body is provided with a heat preservation liquid inlet 112 which can run through the outer frame body of the perfusion container;
the heat preservation liquid outlet 111 and the heat preservation liquid inlet 112 are both communicated with the heat preservation layer, and the effects of inputting and outputting liquid into and out of the heat preservation layer can be achieved.
Generally, in order to achieve the effect of stably inputting the insulating liquid, the insulating liquid outlet 111 and the insulating liquid inlet 112 are respectively communicated with a liquid circulation heating pump or similar fixed temperature liquid conveying device.
In order to realize the updating and replacing effects of the perfusate, the bypass perfusion structure comprises a bypass perfusate inlet 121 and a bypass perfusate outlet 123;
the bypass perfusate inlet 121 is disposed at the upper portion of the perfusion container body, and penetrates through the inner wall of the container (i.e., the inner frame body of the perfusion container) of the perfusion container body, so that the perfusion liquid can be delivered to the perfusion space in the perfusion container body;
the bypass perfusate outlet 123 is disposed at the lower portion of the perfusion container body, and penetrates through the inner wall of the perfusion container body (i.e., the inner frame body of the perfusion container), so that the perfusate in the perfusion container body can be discharged;
in the use process, the bypass perfusate inlet 121 is communicated with the bypass perfusate outlet 123 through a perfusion tube, so that the internal circulation of the perfusate is realized.
In order to realize the effect of updating the perfusion liquid for internal circulation, the perfusion tube is connected with a purifying device, so that the effect of returning the perfusion liquid after updating is realized.
In order to achieve the effect of replacing all or part of the perfusion fluid for internal circulation, a multi-way device can be connected to the perfusion tube, and the effect of returning new perfusion fluid can be achieved through the multi-way device.
The perfusion container body is also provided with a perfusion liquid output port 122;
the perfusion liquid output port 122 is located at a lower portion of the perfusion container body, and penetrates through an inner wall of the container (i.e., a perfusion container inner frame body) of the perfusion container body, so that the perfusion liquid in the perfusion container body can be discharged.
In order to realize the recycling of the perfusate, the bypass perfusate inlet 121, the bypass perfusate outlet 123 and the perfusate outlet 122 can be communicated with a purifying device, so that the discharged liquid is purified and then reused through a perfusion end or a bypass perfusate inlet.
The specific using process is as follows:
s1, placing an isolated organ on a semi-permeable membrane of an objective table 200 (shown in figure 4);
s2, placing the object stage 200 on the filling container 100;
s3, water is fed from a water inlet 112, water is discharged from a water outlet 111, and the temperature of the perfusate in the container is adjusted and controlled by an external heater;
s4, pumping the perfusate out of the perfusate outlet 122, and enabling the perfusate to flow out of the organ and enter the container 100;
s5, when the bypass is needed, the perfusate is pumped out from the perfusate bypass outlet 123 and enters the container from the perfusion pressure bypass inlet 121.
Claims (10)
1. A separated small animal isolated organ perfusion container is characterized in that: comprises a perfusion container body and a bypass perfusion structure;
wherein the filling container body is in a cup shape with an opening part;
the opening part is detachably provided with an object stage;
the bypass perfusion structure comprises a bypass perfusion liquid inlet and a bypass perfusion liquid outlet;
the bypass perfusate inlet and the bypass perfusate outlet are both communicated with the inner wall of the container of the perfusion container body.
2. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the bypass perfusate inlet is arranged above the bypass perfusate outlet.
3. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the bypass perfusate inlet is communicated with the bypass perfusate outlet through a pipeline;
and the pipeline is also connected with a purification mechanism or a multi-way mechanism.
4. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the pouring container body comprises a pouring container inner frame body and a pouring container outer frame body;
and an insulating layer is arranged between the inner frame body of the perfusion container and the outer frame body of the perfusion container.
5. The isolated small animal isolated organ perfusion container of claim 4, wherein:
the device also comprises a heat preservation liquid inlet and a heat preservation liquid outlet;
the heat preservation liquid inlet and the heat preservation liquid outlet are communicated with the outer frame body of the pouring container and communicated with the heat preservation layer.
6. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the pouring container body is also provided with a pouring liquid output structure;
the perfusate output structure is communicated with the inner wall of the container of the perfusion container body.
7. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the bypass perfusate outlet and the perfusate output structure are at the same horizontal height.
8. The isolated small animal isolated organ perfusion container of claim 1, wherein:
the opening part of the pouring container body is provided with a positioning structure;
the positioning structure is matched with the bottom of the object stage.
9. The isolated small animal isolated organ perfusion container of claim 6, wherein:
the bypass perfusate inlet is provided with a valve;
and/or
The bypass perfusate outlet is provided with a valve;
and/or
The perfusate output structure is provided with a valve.
10. A disconnect-type toy isolated organ perfusion subassembly which characterized in that:
comprising the isolated small animal isolated organ perfusion container, stage, and/or decontamination mechanism of any one of claims 1-9.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115530157A (en) * | 2022-11-03 | 2022-12-30 | 河南中医药大学第一附属医院 | Isolated myocardial tissue perfusion device |
CN115777688A (en) * | 2022-11-03 | 2023-03-14 | 河南中医药大学第一附属医院 | Isolated myocardial tissue perfusion experiment system |
CN115812693A (en) * | 2022-11-03 | 2023-03-21 | 河南中医药大学第一附属医院 | Myocardial tissue perfusion experiment processing device |
-
2021
- 2021-02-01 CN CN202120281682.3U patent/CN214758844U/en active Active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115530157A (en) * | 2022-11-03 | 2022-12-30 | 河南中医药大学第一附属医院 | Isolated myocardial tissue perfusion device |
CN115777688A (en) * | 2022-11-03 | 2023-03-14 | 河南中医药大学第一附属医院 | Isolated myocardial tissue perfusion experiment system |
CN115812693A (en) * | 2022-11-03 | 2023-03-21 | 河南中医药大学第一附属医院 | Myocardial tissue perfusion experiment processing device |
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