CN115530157A - Isolated myocardial tissue perfusion device - Google Patents
Isolated myocardial tissue perfusion device Download PDFInfo
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- CN115530157A CN115530157A CN202211374413.7A CN202211374413A CN115530157A CN 115530157 A CN115530157 A CN 115530157A CN 202211374413 A CN202211374413 A CN 202211374413A CN 115530157 A CN115530157 A CN 115530157A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
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- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The invention belongs to the technical field of isolated tissue experimental devices, and particularly discloses an isolated myocardial tissue perfusion device which comprises a perfusion device and a heating mechanism, wherein the heating mechanism comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, a cavity formed by the outer wall and the inner wall is a heating cavity, the outer wall is provided with a water inlet and a water outlet, and the heating cavity is communicated with the outside of the container through the water inlet and the water outlet; the filling mechanism comprises a heating pipe arranged between the inner wall and the outer wall, and the heating pipe is of a spiral structure; the invention provides an in-vitro myocardial tissue perfusion device which is convenient to operate and accurate in experiment.
Description
Technical Field
The invention belongs to the technical field of isolated tissue experimental devices, and particularly relates to an isolated myocardial tissue perfusion device.
Background
The isolated myocardial tissue perfusion device is a set of system which is matched with an isolated heart perfusion experiment. During experiments, the animal heart is taken out, connected to a specific perfusion device, perfused by perfusate, the control of nerves and body fluid is eliminated, the ventricular pressure, the arterial blood pressure and the electrocardiosignals are recorded by matching with special analysis software, various physiological parameters are automatically analyzed, and the device is used for the researches such as the change of the cardiac function and the hemodynamics under the condition of pathophysiology and is widely applied to the physiological, pathophysiology and pharmacological researches; the existing experimental device has more parts and is complex as a whole, and the perfusate is easy to contain gas.
Disclosure of Invention
The invention aims to provide an in-vitro myocardial tissue perfusion device which is convenient to operate and accurate in experiment.
Based on the purpose, the invention adopts the following technical scheme:
an in-vitro myocardial tissue perfusion device comprises a perfusion mechanism and a heating mechanism, wherein the heating mechanism comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, a cavity formed by the outer wall and the inner wall is a heating cavity, a water inlet and a water outlet are formed in the outer wall, and the heating cavity is communicated with the outside of the container through the water inlet and the water outlet; the filling mechanism comprises a heating pipe arranged between the inner wall and the outer wall, and the heating pipe is of a spiral structure.
Further, a liquid inlet communicated with the bottom end opening of the heating pipe is arranged on the outer wall; an exhaust port is arranged at the top of the outer wall, an exhaust pipe is connected between the exhaust port and the opening at the top end of the heating pipe, the exhaust port is connected with the top of the exhaust pipe, the outside of the container is communicated with the exhaust pipe, and gas at the top of the exhaust pipe is exhausted; the sub-unit connection of blast pipe has the liquid outlet, and the liquid outlet setting is on the inner wall, and the liquid outlet communicates blast pipe bottom and container in with.
Further, the filling mechanism comprises a steering pipe, and the steering pipe is arranged in the heating container; the steering tube comprises a transition arc section arranged at the bottom of the heating container, and the transition arc section is arranged between the inner wall and the outer wall; the steering pipe also comprises a liquid inlet straight section and a liquid outlet straight section which are respectively connected with the two ends of the transition arc section; the end of the liquid inlet straight section, which is far away from the transition arc section, is connected with the liquid outlet, the end of the liquid outlet straight section, which is far away from the transition arc section, is provided with a filling interface, the filling interface is arranged at the opening at the top of the heating container, and the liquid outlet straight section is arranged in the heating container and clings to the inner wall.
Furthermore, the device also comprises a negative pressure hose which is connected with a negative pressure pump.
Furthermore, the upper part of the heating container is provided with a wire protection pipe, one end of the wire protection pipe is positioned outside the heating container, and the other end of the wire protection pipe is arranged on the inner wall and communicated with the inside of the heating container.
Further, the water inlet and the water outlet are connected with a circulating water bath heating mechanism; the liquid inlet is connected with a perfusion liquid conveying device.
Furthermore, the heating container, the heating pipe, the steering pipe and the lead protection pipe are all made of transparent glass materials, and the water inlet, the water outlet, the liquid inlet and the liquid outlet are all of tubular structures made of transparent glass materials.
Compared with the prior art, the invention has the following beneficial effects:
1. the heating container top end opening can be used as an experiment platform for placing isolated myocardial tissues, and perfusate can directly flow into the heating container from the top end opening after perfusate is perfused into the isolated myocardial tissues, so that the heating container is convenient to clean. The heating container is arranged to be a double-wall structure to form the heating cavity, and the spiral heating pipe is placed in the heating cavity, so that the heating container can heat the perfusate in the heating pipe. The heating container has the functions of an experiment platform and a heating perfusate, so that the isolated myocardial tissue perfusion device is simpler and is convenient for experiment operation.
2. In the prior art, after the perfusate is perfused into the isolated myocardial tissue, the gas in the perfusate can be perfused into the isolated myocardial tissue, and the gas can influence the accuracy of the experimental result. The exhaust pipe is connected with the opening at the top end of the heating pipe, so that redundant gas in the heated liquid medicine can be exhausted, the gas perfusion into isolated myocardial tissues is reduced, and the experimental result is more accurate.
3. The invention is provided with the steering pipe, and the exhausted perfusate is heated again, so that the temperature of the exhausted perfusate is prevented from being reduced. Simultaneously the drain pipe not with fill interface lug connection, use to turn to the pipe and can set up the interface that fills in heating container open-top's edge with the liquid outlet after filling interface connection, avoid filling the interface and influence placing of separation cardiac muscle tissue.
4. Set up negative pressure hose and negative pressure pump, can take out the perfusate that flows out to heating container from separation cardiac muscle tissue when the experiment, avoid the perfusate that flows to influence the experiment, make the experimentation more stable.
5. The lead protective tube is arranged, and leads for measuring electric signals can penetrate through the lead protective tube to be connected with the isolated myocardial tissues from the heating container.
6. The circulating water bath heating mechanism can keep the temperature in the heating cavity stable, and further keep the temperature of the perfusate stable. The transparent glass material is convenient for observing the experimental process.
Drawings
FIG. 1 is a schematic view of embodiment 1 of the present invention;
fig. 2 is an isometric view of the internal structure of a heating chamber in accordance with embodiment 1 of the present invention;
fig. 3 is a front view of the internal structure of the heating chamber according to embodiment 1 of the present invention;
FIG. 4 is a schematic view showing a use state of the negative pressure tube in embodiment 1 of the present invention;
fig. 5 is a schematic view of a steering tube according to embodiment 1 of the present invention.
In the figure: the device comprises a heating container 1, an inner wall 2, an outer wall 3, a water inlet 4, a water outlet 5, a heating pipe 6, a liquid inlet 7, an exhaust port 8, an exhaust pipe 9, a liquid outlet 10, a transition arc section 11, a liquid inlet straight section 12, a liquid outlet straight section 13, a filling interface 14, a negative pressure hose 15, a negative pressure pump 16, a lead protecting pipe 17 and an adapter pipe 18.
Detailed Description
Example 1
An in-vitro myocardial tissue perfusion device is shown in figures 1-5 and comprises a perfusion device and a heating mechanism, wherein the heating mechanism comprises a heating container 1 with an opening at the top end, as shown in figures 1-2, the heating container 1 is vertically arranged in a cylindrical shape, the heating container 1 is in a double-layer structure comprising an inner wall 2 and an outer wall 3, a cavity formed by the outer wall 3 and the inner wall 2 is a heating cavity, the outer wall 3 is provided with a horizontal water inlet 4 and a horizontal water outlet 5, the water inlet 4 is arranged at the lower part of the outer wall 3, the water outlet 5 is arranged at the upper part of the outer wall 3, and the heating cavity is communicated with the outside of the container through the water inlet 4 and the water outlet 5; the filling mechanism comprises a heating pipe 6 arranged between the inner wall 2 and the outer wall 3, and the heating pipe 6 is of a spiral structure coaxial with the heating container 1.
As shown in fig. 2-3, a liquid inlet 7 communicated with the bottom opening of the heating pipe 6 is arranged at the lower part of the outer wall 3, and the liquid inlet 7 is horizontally arranged; a vertical exhaust port 8 is formed in the top of the outer wall 3, the exhaust port 8 is located above the heating pipe 6, a vertical exhaust pipe 9 is connected between the exhaust port 8 and an opening in the top end of the heating pipe 6, the exhaust port 8 is connected with the top short end of the exhaust pipe 9, the outside of the container is communicated with the exhaust pipe 9 through the exhaust port 8, and gas at the top of the exhaust pipe 9 is exhausted; the lower part of the exhaust pipe 9 is connected with a horizontal liquid outlet 10, the liquid outlet 10 is arranged on the inner wall 2, and the liquid outlet 10 penetrates through the inner wall 2 to communicate the bottom of the exhaust pipe 9 with the inside of the container.
As shown in fig. 2-4, the pouring mechanism comprises a diverting tube disposed inside the heating container 1; the steering tube comprises a transition arc section 11 arranged at the bottom of the heating container 1, the transition arc section 11 is coaxial with the heating tube 6, the transition arc section 11 is arranged between the inner wall 2 and the outer wall 3, and the outer diameter of the transition arc section 11 is slightly smaller than the inner diameter of the inner wall 2; the steering pipe also comprises a liquid inlet straight section 12 and a liquid outlet straight section 13 which are respectively connected with the two ends of the transition arc section 11; the liquid inlet straight section 12 and the liquid outlet straight section 13 are both vertically arranged. The bottom end of the liquid inlet straight section 12 is connected with the transition arc section 11, and the top end is connected with the liquid outlet 10 through the adapter tube 18; the bottom end of the liquid outlet straight section 13 is connected with the transition arc section 11, the top end of the liquid outlet straight section is connected with a vertical filling interface 14, the filling interface 14 is arranged at the edge of the top opening of the heating container 1, and the liquid outlet straight section 13 is arranged in the heating container 1 and clings to the inner wall 2. The top end of the perfusion connector 14 is lower than the top end of the exhaust port 8, so that perfusion liquid is prevented from flowing out of the top of the exhaust pipe 9. The upper portion of heating container 1 is provided with wire protective tube 17, and wire protective tube 17 one end is located outside heating container 1, and the other end sets up on inner wall 2 and communicates in with heating container 1.
As shown in fig. 5, the isolated myocardial tissue perfusion apparatus further includes a negative pressure hose 15, and a negative pressure pump 16 is connected to the negative pressure hose 15. The water inlet 4 and the water outlet 5 are connected with a circulating water bath heating mechanism, and the circulating water bath heating mechanism, the water inlet 4 and the water outlet 5 form a closed loop; the liquid inlet 7 is connected with a perfusion liquid conveying device, and the perfusion liquid conveying device is communicated with the heating pipe 6 through the liquid inlet 7. The circulating water bath heating mechanism and the perfusate conveying device are both in the prior art and are not shown in the figure. The heating container 1, the heating pipe 6, the steering pipe and the lead protective pipe 17 are all made of transparent glass materials, and the water inlet 4, the water outlet 5, the liquid inlet 7 and the liquid outlet 10 are all of tubular structures made of transparent glass materials.
Example 2
This embodiment is an isolated myocardial tissue perfusion device using the following method, comprising the steps of:
step 1, heating a heating cavity; and starting the circulating water bath heating mechanism, continuously pumping hot water into the heating cavity from the water inlet 4, and simultaneously enabling the hot water to flow out of the water outlet 5 to the circulating water bath heating mechanism for reheating so as to keep the temperature in the heating cavity stable.
Step 2, perfusing perfusate; the negative pressure tube is placed at the bottom of the heating container 1, the isolated myocardial tissue connected with the perfusion head is fixed at the opening at the top end of the heating container 1, the perfusion head is connected with the perfusion interface 14, and the measuring instrument is connected to the isolated myocardial tissue. The perfusion fluid conveying device is started, the perfusion fluid is added into the heating pipe 6 through the liquid inlet 7 by the perfusion fluid conveying device, flows through the heating pipe 6, the exhaust pipe 9, the liquid outlet 10, the adapter pipe 18, the liquid inlet straight section 12, the transition arc section 11, the liquid outlet straight section 13, the perfusion interface 14 and the perfusion head in sequence to the isolated myocardial tissue, and then flows out of the isolated myocardial tissue to the heating container 1. The experimental data are measured while the negative pressure pump 16 is activated to pump out the perfusion fluid flowing out into the heating reservoir 1.
Claims (7)
1. An in-vitro myocardial tissue perfusion device comprises a perfusion mechanism and a heating mechanism, and is characterized in that the heating mechanism comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, and a water inlet and a water outlet are formed in the outer wall; the filling mechanism comprises a heating pipe arranged between the inner wall and the outer wall, and the heating pipe is of a spiral structure.
2. The isolated myocardial tissue perfusion apparatus according to claim 1, wherein the outer wall is provided with a liquid inlet communicated with a bottom end opening of the heating tube; the top of outer wall is provided with the gas vent, be connected with the blast pipe between the top end opening of gas vent and heating pipe, the sub-unit connection of blast pipe has the liquid outlet, the liquid outlet sets up on the inner wall.
3. The isolated myocardial tissue perfusion apparatus of claim 2, wherein the perfusion mechanism includes a steering tube; the steering tube comprises a transition arc section arranged at the bottom of the heating container, and the transition arc section is arranged between the inner wall and the outer wall; the steering pipe also comprises a liquid inlet straight section and a liquid outlet straight section which are respectively connected with the two ends of the transition arc section; the one end that transition segmental arc was kept away from to the straight section of feed liquor is connected with the liquid outlet, the one end that transition segmental arc was kept away from to the straight section of play liquid is provided with fills the interface.
4. The isolated myocardial tissue perfusion apparatus of claim 3, further comprising a negative pressure hose to which a negative pressure pump is connected.
5. The isolated myocardial tissue perfusion apparatus of claim 3, wherein the heating container is provided at an upper portion thereof with a wire guard tube, one end of the wire guard tube is located outside the heating container, and the other end of the wire guard tube is disposed on the inner wall and communicates with the inside of the heating container.
6. The isolated myocardial tissue perfusion apparatus of claim 3, wherein the water inlet and outlet are connected to a circulating water bath heating mechanism; the liquid inlet is connected with a perfusion liquid conveying device.
7. The isolated myocardial tissue perfusion apparatus of claim 3, wherein the heating vessel, the heating tube, the steering tube, and the lead guard tube are made of transparent glass material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211374413.7A CN115530157B (en) | 2022-11-03 | 2022-11-03 | Isolated myocardial tissue perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211374413.7A CN115530157B (en) | 2022-11-03 | 2022-11-03 | Isolated myocardial tissue perfusion device |
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| Publication Number | Publication Date |
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| CN115530157A true CN115530157A (en) | 2022-12-30 |
| CN115530157B CN115530157B (en) | 2023-04-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211374413.7A Active CN115530157B (en) | 2022-11-03 | 2022-11-03 | Isolated myocardial tissue perfusion device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190355B1 (en) * | 1992-01-10 | 2001-02-20 | Scimed Life Systems, Inc. | Heated perfusion balloon for reduction of restenosis |
| CN1374138A (en) * | 2002-04-17 | 2002-10-16 | 广元市四一○医院 | Hot perfusion chemotherapeutic apparatus |
| CN204193282U (en) * | 2014-07-11 | 2015-03-11 | 常丽英 | A kind of control by chemotherapy device |
| CN211611281U (en) * | 2019-12-20 | 2020-10-02 | 郑州大学第一附属医院 | Abdominal cavity hot perfusion chemotherapy device |
| CN214758844U (en) * | 2021-02-01 | 2021-11-19 | 无锡市人民医院 | Separated small animal isolated organ perfusion container and assembly |
| CN217219788U (en) * | 2022-04-01 | 2022-08-19 | 深圳市医思美科技有限公司 | Hot filling heating device |
-
2022
- 2022-11-03 CN CN202211374413.7A patent/CN115530157B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190355B1 (en) * | 1992-01-10 | 2001-02-20 | Scimed Life Systems, Inc. | Heated perfusion balloon for reduction of restenosis |
| CN1374138A (en) * | 2002-04-17 | 2002-10-16 | 广元市四一○医院 | Hot perfusion chemotherapeutic apparatus |
| CN204193282U (en) * | 2014-07-11 | 2015-03-11 | 常丽英 | A kind of control by chemotherapy device |
| CN211611281U (en) * | 2019-12-20 | 2020-10-02 | 郑州大学第一附属医院 | Abdominal cavity hot perfusion chemotherapy device |
| CN214758844U (en) * | 2021-02-01 | 2021-11-19 | 无锡市人民医院 | Separated small animal isolated organ perfusion container and assembly |
| CN217219788U (en) * | 2022-04-01 | 2022-08-19 | 深圳市医思美科技有限公司 | Hot filling heating device |
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| CN115530157B (en) | 2023-04-14 |
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