CN211005419U - Automatic liquid changing culture dish for embryo - Google Patents
Automatic liquid changing culture dish for embryo Download PDFInfo
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- CN211005419U CN211005419U CN201921267820.1U CN201921267820U CN211005419U CN 211005419 U CN211005419 U CN 211005419U CN 201921267820 U CN201921267820 U CN 201921267820U CN 211005419 U CN211005419 U CN 211005419U
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Abstract
The utility model discloses an embryo automatic liquid changing culture dish, which comprises at least one group of culture units, wherein each culture unit comprises a liquid injection pool, a culture pool and a waste liquid cavity which are sequentially communicated; the upper part of the liquid injection pool is communicated with a liquid inlet flow channel, and the bottom of the liquid injection pool is communicated with the bottom of the culture pool through an overflow channel; the bottom of the culture pond is provided with at least one embryo micropore, and the bottom of the embryo micropore is communicated to the waste liquid cavity through a waste liquid flow channel. The automatic liquid changing culture dish for the embryos can realize complete liquid changing, and effectively meet the material requirements of the embryos in each development stage; the utility model discloses an automatic liquid changing has avoided the artifical intervention operation repeatedly to the destruction of embryo culture environment and embryo itself injury, can improve embryo culture's stability and blastocyst quality, effectively increases clinical pregnancy rate and live productivity.
Description
Technical Field
The utility model relates to a cell culture field, in particular to automatic liquid culture dish that trades of embryo.
Background
Maintaining a suitable and stable environment for embryo culture system is one of the key factors influencing the result of in vitro fertilization-embryo transfer (IVF-ET). The embryo culture solution is an essential important substance in the process of making a test-tube infant. In the fertilization stage, the sperm and the ovum are combined in vitro to form a fertilized ovum, and then the fertilized ovum is placed into a culture solution for culture, so that the uterus environment can be simulated, and the embryo can grow well. The embryo culture solution not only plays an important role in embryo development, but also has a certain influence on the development of offspring after the embryo is implanted, and has become one of the hot spots for the attention and research in the field of the global Assisted Reproductive Technology (ART).
The common components of human embryo culture solution mainly comprise: (1) water accounts for 99 percent of the total components of the culture solution, and the culture solution is strictly sterilized purified water; (2) inorganic ions, such as calcium ions, magnesium ions and the like, are beneficial to the densification of embryos; (3) energy substrates, which are designed mainly according to the environments of the energy substrates in the oviduct and uterine cavity, such as glucose, pyruvic acid, amino acid and the like; (4) hormones and growth factors, such as luteinizing hormone, follicle stimulating hormone, epidermal growth factor and insulin-like growth factor, can improve the in vitro maturation rate and development potential of oocytes; (5) the protein, currently most added, is Human Serum Albumin (HSA), which has the functions of stabilizing and protecting embryo cell membranes, maintaining stable colloid osmotic pressure, preventing embryos from attaching to embryo culture dishes, and the like; (6) vitamins, regulate cell metabolism, prevent the influence of poor culture conditions on embryo development.
In the process of in vitro culture of embryos, embryos at different stages need to be replaced by different culture solutions, and in a current clinical adopted sequential culture solution system, a semen receiving solution, a cleavage solution and a blastocyst culture solution are designed according to natural changes of the environment in an oviduct, so the cleavage solution and the blastocyst solution need to be replaced respectively on the first day and the third day after oocyte fertilization. The components of the cleavage liquid can meet the nutritional requirements of the embryo on the first to third days, and various components of the blastocyst culture liquid can better meet the material requirements of blastocyst development.
When the culture solution is replaced by a doctor at present, the embryo culture dish which is being cultured needs to be taken out from the incubator every time, the culture solution is sucked by the artificial liquid sucker on the workbench, and then new culture solution is added, so that the operation not only enables the embryo to be separated from the original relatively stable culture environment, but also the embryo itself is easily damaged by artificial liquid sucking intervention action.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to the not enough among the above-mentioned prior art, provide an automatic liquid culture dish that trades of embryo. The problems of damage of embryo culture environment, damage of embryos and the like caused by manual liquid changing operation at present are solved, so that the stability of embryo culture and the quality of blastula are improved, and the clinical conception rate and the survival rate are increased.
In order to solve the technical problem, the utility model discloses a technical scheme is: an embryo automatic liquid changing culture dish comprises at least one group of culture units, wherein each culture unit comprises a liquid injection pool, a culture pool and a waste liquid cavity which are sequentially communicated;
the upper part of the liquid injection pool is communicated with a liquid inlet flow channel, and the bottom of the liquid injection pool is communicated with the bottom of the culture pool through an overflow channel;
the bottom of the culture pond is provided with at least one embryo micropore, and the bottom of the embryo micropore is communicated to the waste liquid cavity through a waste liquid flow channel.
Preferably, the top of the liquid injection pool is provided with a vent hole.
Preferably, an air exhaust port is arranged at the communication part of the waste liquid cavity and the outside.
Preferably, the culture unit is arranged inside the culture dish body, a liquid inlet is formed at the side part of the culture dish body at the inlet end of the liquid inlet flow channel, and the tail end of the liquid inlet flow channel is communicated with the side wall of the upper part of the liquid injection pool.
Preferably, all the liquid inlet flow channels are arranged in the same plane and horizontally, and the liquid inlet flow channels are the same in length from the liquid inlet to the liquid injection pool through the bending layout.
Preferably, the diameter and the depth of each embryo micropore are 0.4-0.5 mm, and each embryo micropore only supports and accommodates a single embryo.
Preferably, the waste liquid flow channel is arranged in a horizontal plane close to the bottom of the embryo micropore, and the inner diameter of the waste liquid flow channel is not more than 0.1 mm.
Preferably, the waste liquid channel comprises a main channel and two branch channels, the inlets of the two branch channels are communicated with the bottoms of the embryo micropores, the tail ends of the two branch channels are converged to the main channel, and the tail end of the main channel is communicated to the waste liquid cavity;
the tail end of the main flow channel is higher than the bottom surface of the waste liquid cavity.
Preferably, the layout and length of the waste fluid flow path from all the embryo wells to the waste chamber are consistent.
Preferably, the two branch flow channels are symmetrically distributed on two sides of the embryo micropore.
The utility model has the advantages that: the automatic liquid changing culture dish for the embryos can realize complete liquid changing, and effectively meet the material requirements of the embryos in each development stage; the utility model discloses an automatic liquid changing has avoided the artifical intervention operation repeatedly to the destruction of embryo culture environment and embryo itself injury, can improve embryo culture's stability and blastocyst quality, effectively increases clinical pregnancy rate and live productivity.
Drawings
FIG. 1 is a perspective view of the automatic liquid-changing embryo culture dish of the present invention;
FIG. 2 is a perspective view of the automatic embryo liquid-changing culture dish according to the present invention;
FIG. 3 is a schematic view of the external structure of the automatic liquid-changing embryo culture dish of the present invention;
FIG. 4 is a side view of the automatic embryo liquid-changing culture dish of the present invention;
fig. 5 is a schematic structural diagram of the waste liquid channel of the present invention.
Description of reference numerals:
1-culture dish body; 2-culture unit; 3-liquid injection pool; 4-culture pond; 5-waste liquid chamber; 6-liquid inlet flow channel; 7-an overflow channel; 8-embryo micropores; 9-waste liquid flow channel; 30-a vent hole; 50-an air extraction opening; 60-liquid inlet; 90-a main runner; 91-branch flow channel; 10-culture solution; 11-oil film.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1-5, the automatic embryo liquid changing culture dish of the present embodiment includes a culture dish body 1, at least one set of culture units 2 is disposed inside the culture dish body 1, and each set of culture units 2 includes a liquid injection tank 3, a culture tank 4 and a waste liquid chamber 5 which are sequentially communicated;
wherein, the upper part of the liquid injection pool 3 is communicated with a liquid inlet flow channel 6, and the bottom of the liquid injection pool is communicated with the bottom of the culture pool 4 through an overflow channel 7;
the bottom of the culture pond 4 is provided with at least one embryo micropore 8, and the bottom of the embryo micropore 8 is communicated to the waste liquid cavity 5 through a waste liquid flow channel 9.
The top of the liquid injection pool 3 is provided with a vent hole 30, so that gas in the liquid inlet flow channel 6 can be discharged in the liquid inlet process, and the surface air pressure of the liquid injection pool 3 and the surface air pressure of the culture pool 4 are balanced.
The inlet end of the liquid inlet flow channel 6 forms a liquid inlet 60 at the side part of the culture dish body 1, and the tail end of the liquid inlet flow channel 6 is communicated with the side wall at the upper part of the liquid injection pool 3. The culture solution reaches the inner side wall of the upper part of the liquid injection pool 3 through the liquid inlet flow channel 6, and through slow supply, the culture solution can be attached to the inner wall of the liquid injection pool 3 and slowly flows down to the liquid level without causing bubbles.
All the liquid inlet flow channels 6 are horizontally arranged in the same plane, and the lengths of all the liquid inlet flow channels 6 from the liquid inlet 60 to the liquid injection pool 3 are the same through the bending layout. The newly changed culture solution of the culture dish is supplied by a multi-channel micro pump or a drip pump through the liquid inlet 60 and enters the liquid inlet flow channel 6, and the lengths of the liquid inlet flow channels 6 are the same, so that the supply flow speed and the volume of the culture solution of each flow channel can be kept consistent.
Overflow channel 7 has been seted up to the bottom of notes liquid pond 3, communicates with each other with culture pond 4 bottom, and when new culture solution injected notes liquid pond 3, the liquid level of notes liquid pond 3 can be raised gradually, appears certain difference in height with culture pond 4 liquid level, and under the effect of gravity and outside atmospheric pressure, new culture solution can flow to culture pond 4 in through overflow channel 7 in the notes liquid pond 3.
The culture pond 4 contains culture solution, the period is long during embryo culture, and in order to prevent the culture solution from volatilizing, a mineral oil film 11 is required to cover the upper layer when in use.
The diameter and the depth of each embryo micropore 8 are both 0.4-0.5 mm, and each embryo micropore 8 only supports and holds a single embryo.
The waste liquid flow channel 9 is arranged in a horizontal plane close to the bottom of the embryo micropore 8, and the inner diameter of the waste liquid flow channel 9 is not more than 0.1 mm. In contrast, the diameter of the embryos is typically about 0.2mm to 0.3mm, which ensures that the embryos do not flow into the waste channel 9.
The bottom surface of the waste liquid cavity 5 is lower than the outlet of the waste liquid flow channel 9, and the total volume from the outlet of the waste liquid flow channel 9 to the height range of the bottom surface of the waste liquid cavity 5 is used as the maximum allowable waste liquid storage amount of the culture dish.
The waste liquid channel 9 includes a main channel 90 and two branch channels 91, the inlets of the two branch channels 91 are communicated with the bottom of the embryo micropore 8, and the two branch channels 91 are distributed at a certain angle, preferably 180 ° (i.e. symmetrically distributed at two sides of the embryo micropore 8). The ends of the two flow channels 91 converge to the main flow channel 90, and the end of the main flow channel 90 communicates with the waste liquid chamber 5. The layout and length of all embryo wells 8 to the waste channel 9 of the waste chamber 5 remains the same. The two branch flow channels 91 are arranged, so that the problem that a single inlet is easily blocked by the embryo to cause difficulty in discharging waste liquid can be effectively solved, and the problem that the embryo is damaged by negative pressure increase caused by blocking under more serious conditions can be solved.
An air pumping hole 50 is arranged at the communicating part of the waste liquid cavity 5 and the outside. The culture medium discharging device is used for connecting a negative pressure pump, the air pressure in the waste liquid cavity 5 is obviously lower than the external air pressure under the air exhaust action of the negative pressure pump, the culture medium in the culture pond 4 is discharged into the waste liquid cavity 5 from the branch flow channel 91 at the bottom of the micropore through the waste liquid flow channel 9 under the action of pressure difference, and the air pressure in the space of the waste liquid cavity 5 is uniform, and the layout and the length of each waste liquid flow channel 9 are the same, so that the difference of the waste liquid flow discharged from each embryo micropore 8 is small, and the consistency of the waste liquid discharge is good.
Wherein, the material of culture dish can adopt glass, quartzy and macromolecular polymer etc. or its combined material to constitute, and conventional method is adopted to the manufacturing process of miniflow channel part (especially waste liquid runner 9), like the bonding method of similar micro-fluidic chip, and other structure parts can adopt 3D to print or injection moulding, the utility model discloses do not improve here.
The utility model discloses an automatic liquid culture dish that trades of embryo, it is similar with the preparation flow of conventional culture dish before the use, need artifical interpolation culture solution, get rid of the visible bubble in the culture solution, cover anti-volatilization mineral oil film 11, leading-in embryo, wherein need stop up bleeding hole 50 when adding the culture solution to avoid the culture solution too much to flow into in the waste liquid runner 9, or gas in the waste liquid runner 9 overflows from the micropore and arouses the bubble. Finally, the prepared culture dish is placed in a matched time difference imaging culture box or a special embryo culture box with a real-time monitoring system, and then the liquid inlet 60 and the air extraction opening 50 of the culture dish are respectively connected with corresponding external gas-liquid pipeline ports to finish the operation.
In one embodiment, the utility model discloses an automatic liquid process of trading of embryo's automatic liquid culture dish does: the negative pressure pump is connected with the air extraction opening 50, firstly, the negative pressure pump is controlled to enable the waste liquid cavity 5 to form a uniform and proper negative pressure space, partial culture liquid in the culture pond 4 is forced to be discharged into the waste liquid cavity 5 from the bottom of the embryo micropore 8 through the waste liquid flow channel 9, then, the multichannel micropump or the drip pump is controlled to supply new culture liquid, the culture liquid reaches the liquid injection pond 3 through the liquid inlet flow channel 6, the culture liquid is attached to the inner wall of the liquid injection pond 3 and slowly flows down to the liquid level, the new culture liquid flows into the culture pond 4 through the overflow channel 7 under the action of gravity along with the lifting of the liquid level, the concentration of the new culture liquid can be gradually increased, and the more thorough liquid changing effect can be completed through repeated cycles.
While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields where the invention is suitable, and further modifications may readily be made by those skilled in the art, and the invention is therefore not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. An embryo automatic liquid changing culture dish is characterized by comprising at least one group of culture units, wherein each culture unit comprises a liquid injection pool, a culture pool and a waste liquid cavity which are sequentially communicated;
the upper part of the liquid injection pool is communicated with a liquid inlet flow channel, and the bottom of the liquid injection pool is communicated with the bottom of the culture pool through an overflow channel;
the bottom of the culture pond is provided with at least one embryo micropore, and the bottom of the embryo micropore is communicated to the waste liquid cavity through a waste liquid flow channel.
2. The automatic liquid change culture dish of embryo according to claim 1, wherein, the top of annotating the liquid pond and having seted up the air vent.
3. The automatic liquid-changing culture dish for embryos of claim 1, wherein an air pumping port is arranged at the communication part of the waste liquid cavity and the outside.
4. The automatic liquid-changing culture dish for the embryos according to claim 1, wherein the culture unit is arranged inside a culture dish body, a liquid inlet is formed at the side part of the culture dish body at the inlet end of the liquid inlet flow channel, and the tail end of the liquid inlet flow channel is communicated with the side wall of the upper part of the liquid injection pool.
5. The automatic liquid changing culture dish for embryos according to claim 4, wherein all the liquid inlet flow channels are horizontally arranged in the same plane, and the lengths of the liquid inlet flow channels from the liquid inlet to the liquid injection pool are the same through a bending layout.
6. The automatic embryo liquid changing culture dish according to claim 1, wherein the diameter and the depth of each embryo micropore are 0.4-0.5 mm, and each embryo micropore only supports and holds a single embryo.
7. The automatic liquid changing culture dish for embryos of claim 1, wherein the waste liquid channel is arranged in a horizontal plane near the bottoms of the micropores of the embryos, and the inner diameter of the waste liquid channel is not more than 0.1 mm.
8. The automatic liquid changing culture dish for embryos according to claim 7, wherein the waste liquid channel comprises a main channel and two branch channels, the inlets of the two branch channels are communicated with the bottoms of the embryo micropores, the tail ends of the two branch channels are converged to the main channel, and the tail end of the main channel is communicated to the waste liquid cavity;
the tail end of the main flow channel is higher than the bottom surface of the waste liquid cavity.
9. The automatic embryo changing dish according to claim 8, wherein the layout and length of the waste liquid flow path from all the embryo micropores to the waste liquid chamber are consistent.
10. The automatic liquid changing culture dish for embryo according to claim 9, wherein the two branch flow paths are symmetrically distributed on two sides of the embryo micropore.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921267820.1U CN211005419U (en) | 2019-08-07 | 2019-08-07 | Automatic liquid changing culture dish for embryo |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921267820.1U CN211005419U (en) | 2019-08-07 | 2019-08-07 | Automatic liquid changing culture dish for embryo |
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| CN211005419U true CN211005419U (en) | 2020-07-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408540A (en) * | 2019-08-07 | 2019-11-05 | 中国科学院苏州生物医学工程技术研究所 | Embryo's changing liquid automatically culture dish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408540A (en) * | 2019-08-07 | 2019-11-05 | 中国科学院苏州生物医学工程技术研究所 | Embryo's changing liquid automatically culture dish |
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