CN201251576Y - Isolated cell perfusion device for a microscope carrier - Google Patents
Isolated cell perfusion device for a microscope carrier Download PDFInfo
- Publication number
- CN201251576Y CN201251576Y CNU2008200303413U CN200820030341U CN201251576Y CN 201251576 Y CN201251576 Y CN 201251576Y CN U2008200303413 U CNU2008200303413 U CN U2008200303413U CN 200820030341 U CN200820030341 U CN 200820030341U CN 201251576 Y CN201251576 Y CN 201251576Y
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- Prior art keywords
- major trough
- perfusate
- drain pipe
- perfusion device
- pipe
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an isolated cell perfusion device for a microscope carrier which comprises a base and is characterized in that the centre of the upper side of the base is provided with a major trough and a covered channel arranged at the bottom of the major through; the upper part of the manor trough is provided with a drain pipe which is communicated with a negative pressure pipe and at least a liquid inlet pipe; the covered channel is communicated with a negative draught tube. In the utility model, as the major trough has smaller volume, cell perfusion process can be finished in short time by using little perfusate, thus saving perfusate and medicine; a multitrack liquid inlet pipe is adopted, which is convenient for replacing multiple medicine perfusate in experiment, thus reducing inaccuracy error of experimental results, increasing accuracy rate and success ratio of experiment, saving experimental expenses and reducing experimental cost while increasing work efficiency and performance quality.
Description
Technical field
The utility model relates to a kind of preclinical medicine experimental provision, particularly relates to a kind of isolated cells perfusion device that is used on the microscope stage.
Background technology
At present in the preclinical medicine field, in the patch-clamp record experiment for ion channel, perfusion at isolated cells is to adopt plasticine double dish to be fixed on the inverted microscope objective table temporarily, shortcoming be improper owing to putting, put reasons such as shakiness, cause displacement easily, cause electrode and cell detachment, influenced normally carrying out of experiment.In addition, because the volume of Tissue Culture Dish is bigger, at the cell perfusion with in changing dressings, the perfusate volume that needs and the amount of medicine are bigger, especially to some valuable medicine, can cause waste; Also have, single perfusion pipeline is used for the perfusion experiment of multiple medicine, can only be by repeatedly changing the perfusate container and repeatedly moving instrument adjustment, be consuming time inconvenient again like this, increased a lot of workloads virtually, sometimes even may cause that experiment is interrupted, failure, make accuracy rate, the success ratio of experimental result all be difficult to be guaranteed.
Summary of the invention
The purpose of this utility model provides a kind of cell perfusion device that is used on the inverted microscope objective table, the perfusion and the electrophysiology experiment of isolated cells can be carried out, existing interim double dish can be solved because of problems such as fixing difficulty, replacing perfusate inconvenience and perfusate wastes.
For reaching above purpose, the utility model takes following technical scheme to be achieved:
A kind of isolated cells perfusion device that is used on the microscope stage comprises pedestal, it is characterized in that, the middle part is provided with the undercut of major trough and major trough bottom above the described pedestal; Described major trough top is provided with drain pipe, negative pressure pipe of this drain pipe UNICOM and at least one feed tube; Negative-pressure drainage tube of described undercut UNICOM.
In the such scheme, described major trough one side is provided with overflow groove, and this overflow groove end enters waste liquid bottle by a spout hole that connects the pedestal top and bottom with unnecessary liquid at least.Described drain pipe, feed tube and negative pressure pipe are arranged vertically, and drain pipe and feed tube are arranged dorsad.
The beneficial effects of the utility model: (1) perfusion device is fixed on the objective table of inverted microscope by mounting hole, has avoided scratching in the experiment, the cell that causes of changing dressings to move; (2) the major trough volume is less, just can finish the cell perfusing course at short notice with less perfusate, saves perfusate and medicine; (3) multiple tracks feed tube is convenient to be convenient for changing multiple drug perfusion liquid in the experiment, so the utility model is improving work efficiency and work quality simultaneously, owing to reduce the error of experimental result, improve accuracy rate, the success ratio of experiment, saved experimental expenses, reduced experimental cost.
Description of drawings
Fig. 1 is a front elevation of the present utility model.
Fig. 2 is that the A-A of Fig. 1 is to cut-open view.
Among Fig. 1 to Fig. 2: 1, major trough; 2, spout hole; 3, pedestal; 4, mounting hole; 5, undercut; 6, negative-pressure drainage tube; 7, flexible pipe; 8, overflow groove; 9, negative pressure pipe; 10, drain pipe; 11, feed tube; 12, base.
Embodiment
Below in conjunction with drawings and Examples the utility model is described in further detail.
As shown in Figure 1 and Figure 2, a kind of isolated cells perfusion device that is used on the microscope stage comprises pedestal 3, and the middle part is provided with major trough 1, the undercut 5 of major trough bottom and the overflow groove 8 of major trough one side above the pedestal; The major trough top is provided with drain pipe 10, negative pressure pipe of this drain pipe UNICOM 9 and at least one feed tube 11, and present embodiment is provided with four feed tubes; Drain pipe 10, negative pressure pipe 9 and feed tube 11 are fixed on the pedestal of major trough 1 one sides by a base 12; Drain pipe 10, feed tube 11 is arranged vertically with negative pressure pipe 9, and drain pipe and feed tube are arranged dorsad.Negative-pressure drainage tube 6 of undercut 5 UNICOMs of major trough bottom, this negative-pressure drainage tube is fixed on the pedestal of major trough 1 opposite side by screw.Overflow groove 8 terminal two forks that divide can enter waste liquid bottle with unnecessary liquid by two spout holes 2 that connect the pedestal top and bottom.
Principle of work of the present utility model is: pedestal 3 is fixed in the objective table of inverted microscope by the mounting hole 4 around it, single cell suspension or the glass cover slide of climbing full cell are positioned in the major trough 1, the cell perfusate flows into by feed tube 11, and drain pipe 10 flows out to major trough 1; Negative pressure pipe 9 is connected by flexible pipe 7 with first syringe, can take ducted air away with negative pressure for feed tube 11 by first syringe and helps the liquid inflow.Various drug perfusion liquid can be changed easily by multiple tracks feed tube 11, and multiple drug perfusion liquid also can pour into to major trough 1 by these multiple tracks medicine feed tube 11 merging.Negative-pressure drainage tube 6 is connected by flexible pipe 7 with second syringe, helping liquid by the controlled imbibition of second syringe flows into, simultaneously major trough 1 perfusate from undercut 5 from the negative-pressure drainage tube sucking-off, thereby realize the extracellular fluid perfusion, too much liquid can enter waste liquid bottle from the spout hole 2 of overflow groove 8 ends.
Claims (3)
1, a kind of isolated cells perfusion device that is used on the microscope stage comprises pedestal, it is characterized in that, the middle part is provided with the undercut of major trough and major trough bottom above the described pedestal; Described major trough top is provided with drain pipe, negative pressure pipe of this drain pipe UNICOM and at least one feed tube; Negative-pressure drainage tube of described undercut UNICOM.
2, the isolated cells perfusion device that is used on the microscope stage according to claim 1, it is characterized in that, described major trough one side is provided with overflow groove, and this overflow groove end enters waste liquid bottle by a spout hole that connects the pedestal top and bottom with unnecessary liquid at least.
3, the isolated cells perfusion device that is used on the microscope stage according to claim 1 and 2 is characterized in that, described drain pipe, feed tube and negative pressure pipe are arranged vertically, and drain pipe and feed tube are arranged dorsad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200303413U CN201251576Y (en) | 2008-09-19 | 2008-09-19 | Isolated cell perfusion device for a microscope carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200303413U CN201251576Y (en) | 2008-09-19 | 2008-09-19 | Isolated cell perfusion device for a microscope carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201251576Y true CN201251576Y (en) | 2009-06-03 |
Family
ID=40747248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200303413U Expired - Fee Related CN201251576Y (en) | 2008-09-19 | 2008-09-19 | Isolated cell perfusion device for a microscope carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201251576Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103536277A (en) * | 2013-10-30 | 2014-01-29 | 中南民族大学 | Automated ultra-weak light imaging system and operation method |
| WO2020118727A1 (en) * | 2018-12-14 | 2020-06-18 | 中国科学院深圳先进技术研究院 | Cellular recording chamber device and cellular recording method |
| CN111708161A (en) * | 2020-06-08 | 2020-09-25 | 南通大学 | A antifouling device of microscope objective table for physiology experiment |
-
2008
- 2008-09-19 CN CNU2008200303413U patent/CN201251576Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103536277A (en) * | 2013-10-30 | 2014-01-29 | 中南民族大学 | Automated ultra-weak light imaging system and operation method |
| WO2020118727A1 (en) * | 2018-12-14 | 2020-06-18 | 中国科学院深圳先进技术研究院 | Cellular recording chamber device and cellular recording method |
| CN111708161A (en) * | 2020-06-08 | 2020-09-25 | 南通大学 | A antifouling device of microscope objective table for physiology experiment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090603 Termination date: 20091019 |