CN208684977U - A kind of multi-layer cellular culture bottle - Google Patents
A kind of multi-layer cellular culture bottle Download PDFInfo
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- CN208684977U CN208684977U CN201820807597.4U CN201820807597U CN208684977U CN 208684977 U CN208684977 U CN 208684977U CN 201820807597 U CN201820807597 U CN 201820807597U CN 208684977 U CN208684977 U CN 208684977U
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- culturing room
- culture
- culture bottle
- diversion pipe
- bottleneck
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Abstract
The utility model discloses a kind of multi-layer cellular culture bottles, including culture bottle, placing groove, culturing room one, culturing room two, culture bottleneck, diversion pipe, sealing plate, through-hole, pulling block, pull rod, knob, drainage tube, inflow pipe.The utility model has the beneficial effects that the utility model stacks culturing room one and multiple culturing room two in vertical direction, make there are multiple culturing room in a culture bottle, culture solution containing cell is drained into multiple culturing room two by culturing room one, it does not need pipette and passes through each culturing room, reduce the operating time, reduce pollution risk, reduce the operating time, reduce occupied space, pulling block is provided in culturing room one and culturing room two, pulling block can block culture bottleneck, it avoids mixing, pulling block can pull, situation is flowed into control culture solution outflow containing cell, it can be according to practical control.
Description
Technical field
The utility model relates to a kind of Tissue Culture Flask, specially a kind of multi-layer cellular culture bottle belongs to cell training
Support technical field.
Background technique
Cell culture is the core of biotechnology, and Tissue Culture Flask is the usual means of cell culture, because it can
To cultivate adherent cell type, non-adherent cells type can also be cultivated.Conventional flask has a culturing room, provide compared with
Small culture volume and culture surface product, and biggish space is occupied, lead to the inefficient utilization to space, and increase work
Amount and pollution risk.
Multilayer culture flask, which stacks culture layer in vertical direction, makes have multiple culturing room in a culture bottle.Culture medium can lead to
It crosses and shares Rendezvous Point pick-and-place, do not need pipette by each culturing room, so as to which so that the height of culturing room reduces, diminution is each
The space of a culturing room keeps the area of unit volume inner cell culture bigger, reduces the operating time, reduces pollution risk, solves
The problem of conventional flask.
Utility model content
The purpose of this utility model is that above-mentioned since conventional flask can only provide lesser culture medium in order to solve
Volume and culture surface product, and biggish space is occupied, lead to the inefficient utilization to space, and workload is beaten and existed dirty
Contaminate risk the problem of and a kind of multi-layer cellular culture bottle is provided.
The utility model is achieved through the following technical solutions above-mentioned purpose, a kind of multi-layer cellular culture bottle, including
Culture bottle, the culture bottle are internally provided with placing groove, and the placing groove is internally provided with culturing room one and multiple
Culturing room two not of uniform size, multiple culturing room two stack gradually setting from big to small, and the culturing room one is arranged most
Culture bottleneck, the adjacent training are provided on the side wall of the upper end of small culturing room two, the culturing room one and culturing room two
It supports and is connected with diversion pipe between bottleneck, the rear end of the diversion pipe extends to the outside of culture bottle, and the diversion pipe is horizontally disposed,
The end of the diversion pipe is fixed with sealing plate, and through-hole is provided on the sealing plate, and the diversion pipe is internally provided with drawing
Block is connected with pull rod on the outside of the pulling block, and the end of the pull rod passes through through-hole, and the end of pull rod is provided with knob, more
It is connected with vertical drainage tube between a adjacent guide pipe, is provided with inflow on the culture bottleneck in culturing room two
Pipe, the inside for flowing into pipe and being located at culturing room two.
Preferably, when not shifting culture solution containing cell, pulling block can block culture bottleneck, described to flow into the straight of pipe
Diameter is not less than the diameter of culture bottleneck, and the diameter of the pulling block is greater than the diameter for cultivating bottleneck.
Preferably, in order to pull pull rod, the amount for increasing leakproofness and control culture solution containing cell being facilitated to flow out, institute
It states pull rod to be slidably connected with sealing plate, is coated with Anti-slip cover on the side wall of the through-hole.
Preferably, in order to have buffer function when pulling pulling block, facilitate control culture solution outflow containing cell
Amount, is provided with spring, the spring is set on pull rod between the sealing plate and pulling block.
Preferably, in order to play antipollution effect, at the same facilitate record cell culture time, the culture bottle it is upper
End is provided with bottle cap, and the bottle cap is threadedly coupled with culture bottle, is provided with time regulator on the bottle cap.
Preferably, in order to be not in refluence when culture solution containing cell is flowed into each culturing room two the case where,
Simultaneously in order to which culture solution containing cell in culturing room one can be assigned in multiple culturing room two, each training is in addition conveniently checked
The inflow situation of culture solution in room two is supported, the bottom for flowing into pipe is higher than the interior bottom portion of culturing room two, the culturing room one
Depth be greater than each culturing room two depth, the smallest culturing room two of width be greater than culturing room one width.
Preferably, culture solution containing cell in culturing room one is flowed out for convenience from drainage tube, is located at culturing room one
On the drainage tube be located at the inside of diversion pipe, and the upper end of drainage tube is flushed with the inner sidewall of diversion pipe.
Preferably, in order to when outside is seen and drains into the culture solution containing cell in culturing room one in culturing room two
The case where, the culture bottle is made of transparent glass material.
Compared with prior art, the utility model has the beneficial effects that
1, the utility model stacks culturing room one and multiple culturing room two in vertical direction, makes to have in a culture bottle more
Culture solution containing cell is drained into multiple culturing room two by culturing room one, is not needed pipette and pass through by a culturing room
Each culturing room reduces the operating time, reduces pollution risk, keeps the area of unit volume inner cell culture bigger, reduces operation
Time reduces occupied space.
2, the utility model is provided with pulling block in culturing room one and culturing room two, and pulling block can block culture bottleneck,
Avoid mixing, pulling block can pull, so that controlling culture solution containing cell outflow flows into situation, can according to practical control,
Improve effective use of the conventional cell culture flasks to space.
Detailed description of the invention
Fig. 1 is the utility model overall structure diagram.
Fig. 2 is the utility model cross section structure schematic diagram.
In figure: 1, bottle cap；2, culture bottle；3, diversion pipe；4, sealing plate；5, pull rod；6, it shakes hands；7, culturing room one；8, it trains
Support room two；9, pulling block；10, pipe is flowed into；11, drainage tube；12, spring；13, bottleneck is cultivated.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Embodiment 1
It please refers to shown in Fig. 1-Fig. 2, a kind of multi-layer cellular culture bottle, including culture bottle 2, the inside of culture bottle 2 are set
It is equipped with placing groove, placing groove is internally provided with a culturing room 1 and multiple sizes of culturing room 28, multiple cultures
Room 28 stacks gradually setting from big to small, and can increase culture bottle 2 utilizes space, makes the face of unit volume inner cell culture
Product is bigger, reduces the operating time, reduces occupied space, and the upper end of the smallest culturing room 28, culturing room is arranged in culturing room 1
1 and culturing room 28 side wall on be provided with culture bottleneck 13, each culture bottleneck 13 is connected with diversion pipe 3, diversion pipe 3
Rear end extend to the outside of culture bottle 2, culture solution outflow containing cell while being flowed into culturing room 28 in culturing room 1
Interior, diversion pipe 3 is horizontally disposed, and the end of diversion pipe 3 is fixed with sealing plate 4, prevents culture solution containing cell from flowing to outside, close
Through-hole is provided on sealing plate 4, diversion pipe 3 is internally provided with pulling block 9, and the outside of pulling block 9 is connected with pull rod 5, the end of pull rod 5
The end of through-hole, and pull rod 5 is provided with knob 6, pulling block 9 is for blocking culture bottleneck 13, by pulling the control of pulling block 9 to contain
There are the culture solution discharge and influx of cell, be connected with vertical drainage tube 11 between adjacent guide pipe 3, in culturing room 28
Culture bottleneck 13 on be provided with and flow into pipe 10, flow into pipe 10 and be located at the inside of culturing room 28, can containing culturing room 1
There is the culture solution of cell to drain into multiple culturing room 28, do not need pipette by each culturing room, reduce the operating time,
Reduce pollution risk.
Embodiment 2
In addition,-Fig. 2 flows into diameter of the diameter as many as culture bottleneck 13 of pipe 10 based on the above embodiment referring to Fig.1,
The diameter of pulling block 9 is greater than the diameter of culture bottleneck 13, and the pulling block 9 when not shifting culture solution containing cell is allow to block culture
Bottleneck 13, pull rod 5 are slidably connected with sealing plate 4, Anti-slip cover are coated on the side wall of through-hole, in order to facilitate the pulling of pull rod 5, simultaneously
Increase leakproofness and facilitate the amount for controlling culture solution outflow containing cell, is provided with spring between sealing plate 4 and pulling block 9
12, spring 12 is set on pull rod 5, in order to there is buffer function when pulling pulling block 9, facilitates control culture containing cell
The amount that liquid stream goes out, the upper end of culture bottle 2 are provided with bottle cap 1, and bottle cap 1 is threadedly coupled with culture bottle 2, and having time is arranged on bottle cap 1
Logger in order to play antipollution effect, while facilitating the time of record cell culture, and the bottom for flowing into pipe 10 is higher than culture
The interior bottom portion of room 28, the depth of culturing room 1 are greater than the depth of each culturing room 28, and the smallest culturing room 28 of width is big
In the width of culturing room 1, make be not in when culture solution containing cell is flowed into each culturing room 28 refluence feelings
Condition, while in order to which culture solution containing cell in culturing room 1 can be assigned in multiple culturing room 28, in addition conveniently check
The inflow situation of culture solution in each culturing room 28, the drainage tube 11 in culturing room 1 are located at the inside of diversion pipe 3, and
The upper end of drainage tube 11 is flushed with the inner sidewall of diversion pipe 3, makes to facilitate by culture solution containing cell in culturing room 1 from drainage
Pipe 11 flows out, and culture bottle 2 is made of transparent glass material, and making to see in outside will be containing cell in culturing room 1
The case where when culture solution drains into culturing room 28.
When the utility model is used, stacking culturing room 1 and multiple culturing room 28 in vertical direction, make a culture
There are multiple culturing room in bottle 2, culture solution containing cell is drained into multiple culturing room 28 by culturing room 1, is not needed
Pipette reduces the operating time by each culturing room, reduces pollution risk, makes the area of unit volume inner cell culture more
Greatly, the operating time is reduced, reduces occupied space, is provided with pulling block 9 in culturing room 1 and culturing room 28, pulling block 9 can be with
Culture bottleneck 13 to be blocked, avoids mixing, pulling block 9 can pull, so that controlling culture solution outflow containing cell flows into situation, it can
To improve effective use of the conventional cell culture flasks 2 to space according to practical control.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (8)
1. a kind of multi-layer cellular culture bottle, which is characterized in that including culture bottle (2), the inside of the culture bottle (2) is arranged
There is a placing groove, the placing groove is internally provided with a culturing room one (7) and multiple sizes of culturing room two (8), more
A culturing room two (8) stacks gradually setting from big to small, and the culturing room one (7) is arranged in the smallest culturing room two (8)
Upper end, be provided with culture bottleneck (13), each culture on the side wall of the culturing room one (7) and culturing room two (8)
Bottleneck (13) is connected with diversion pipe (3), and the rear end of the diversion pipe (3) extends to the outside of culture bottle (2), the diversion pipe
(3) it is horizontally disposed with, the end of the diversion pipe (3) is fixed with sealing plate (4), is provided with through-hole on the sealing plate (4), described
Diversion pipe (3) is internally provided with pulling block (9), is connected with pull rod (5) on the outside of the pulling block (9), the end of the pull rod (5)
It is provided with knob (6) across the end of through-hole, and pull rod (5), is connected with vertical drainage tube between the adjacent diversion pipe (3)
(11), inflow pipe (10) is provided on the culture bottleneck (13) in culturing room two (8), the inflow pipe (10) is located at culture
The inside of room two (8).
2. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: described to flow into the straight of pipe (10)
Diameter is not less than the diameter of culture bottleneck (13), and the diameter of the pulling block (9) is greater than the diameter for cultivating bottleneck (13).
3. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: the pull rod (5) and sealing
Plate (4) is slidably connected, and is coated with Anti-slip cover on the side wall of the through-hole.
4. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: the sealing plate (4) and drawing
It is provided between block (9) spring (12), the spring (12) is set on pull rod (5).
5. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: the culture bottle (2) it is upper
End is provided with bottle cap (1), and the bottle cap (1) is threadedly coupled with culture bottle (2), is provided with time regulator on the bottle cap (1).
6. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: the bottom for flowing into pipe (10)
Portion is higher than the interior bottom portion of culturing room two (8), and the depth of the culturing room one (7) is greater than the depth of each culturing room two (8)
Degree, the smallest culturing room two (8) of width are greater than the width of culturing room one (7).
7. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: be located in culturing room one (7)
The drainage tube be located at the inside of diversion pipe (3), and the upper end of drainage tube (11) is flushed with the inner sidewall of diversion pipe (3).
8. a kind of multi-layer cellular culture bottle according to claim 1, it is characterised in that: the culture bottle (2) uses
Transparent glass material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820807597.4U CN208684977U (en) | 2018-05-26 | 2018-05-26 | A kind of multi-layer cellular culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820807597.4U CN208684977U (en) | 2018-05-26 | 2018-05-26 | A kind of multi-layer cellular culture bottle |
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| Publication Number | Publication Date |
|---|---|
| CN208684977U true CN208684977U (en) | 2019-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820807597.4U Active CN208684977U (en) | 2018-05-26 | 2018-05-26 | A kind of multi-layer cellular culture bottle |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518692A (en) * | 2020-04-30 | 2020-08-11 | 海口健康岛生物科技有限公司 | Automatic human stem cell culture instrument |
| CN112852594A (en) * | 2021-01-21 | 2021-05-28 | 刘寿明 | Cell culture device for biotechnology research |
-
2018
- 2018-05-26 CN CN201820807597.4U patent/CN208684977U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518692A (en) * | 2020-04-30 | 2020-08-11 | 海口健康岛生物科技有限公司 | Automatic human stem cell culture instrument |
| CN112852594A (en) * | 2021-01-21 | 2021-05-28 | 刘寿明 | Cell culture device for biotechnology research |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 321000 room A201, South Tower, Mei Baolong center, 639 Yongkang street, Wucheng, Jinhua, Zhejiang Patentee after: Zhejiang SDAM Stem Cell Biotechnology Co., Ltd Address before: 321000 room A201, South Tower, Mei Baolong center, 639 Yongkang street, Wucheng, Jinhua, Zhejiang Patentee before: Jinhua Si damm Stem Cell Biotechnology Co. Ltd. |