CN213357615U - Water-sealed anti-pollution stem cell amplification device - Google Patents
Water-sealed anti-pollution stem cell amplification device Download PDFInfo
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- CN213357615U CN213357615U CN202022109121.3U CN202022109121U CN213357615U CN 213357615 U CN213357615 U CN 213357615U CN 202022109121 U CN202022109121 U CN 202022109121U CN 213357615 U CN213357615 U CN 213357615U
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- water
- water seal
- stem cell
- pollution
- end cover
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 33
- 230000003321 amplification Effects 0.000 title claims abstract description 10
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 58
- 238000007789 sealing Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 7
- 230000010261 cell growth Effects 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000010030 laminating Methods 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 19
- 210000004027 cell Anatomy 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 239000011148 porous material Substances 0.000 abstract description 4
- 230000001771 impaired effect Effects 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 210000003014 totipotent stem cell Anatomy 0.000 description 1
- 210000002444 unipotent stem cell Anatomy 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an anti-pollution stem cell amplification device of water seal relates to biological cell technical field, including the blake bottle, the upper end of blake bottle is provided with the end cover, and the end cover passes through screw thread and blake bottle fixed connection, is provided with sealed the pad between end cover and the blake bottle, and the middle part through connection of end cover has the hollow tube, hollow tube and end cover fixed connection, and the lower extreme of hollow tube is provided with the water seal piece, and the water seal groove has been seted up to the upper surface of water seal piece, the lower extreme of hollow tube and the bottom inner wall fixed connection in water seal groove. The utility model discloses a water seal tank has been seted up at the upper surface of water seal block, the bottom inner wall in water seal tank and the lower extreme fixed connection of hollow tube, the intercommunicating pore has been seted up to the inside wall lower extreme of hollow tube, the annular plate has been cup jointed in the lower extreme outside of hollow tube, the nutrient solution that the annular plate inner chamber was stored can play the water seal effect to the inner chamber of intercommunicating pore to avoid the outside air to get into the inner chamber of blake bottle, and then avoided the stem cell to receive the pollution of bacterium in the air.
Description
Technical Field
The utility model relates to a biological cell technical field, in particular to anti-pollution stem cell amplification device of water seal.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells. Stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, are called as universal cells in medical science, and are expanded: separating and purifying autologous, allogeneic or xenogeneic stem cells, and culturing the stem cells under aseptic conditions to increase the number of the stem cells.
At present, when a nutrient solution is filled into the existing stem cell amplification device, a bottle cap is usually required to be opened, bacteria in the air enter the bottle while the bacteria enter the bottle, and stem cells are easily polluted, so that the amplification of the stem cells is easily influenced. Therefore, it is necessary to develop a water-sealed and contamination-proof stem cell expansion device to solve the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an anti-pollution stem cell amplification device of water seal to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a water seal anti-pollution stem cell amplification device comprises a culture bottle, wherein an end cover is arranged at the upper end of the culture bottle and fixedly connected with the culture bottle through threads, a sealing gasket is arranged between the end cover and the culture bottle, a hollow pipe is connected to the middle of the end cover in a penetrating manner and fixedly connected with the end cover, a water seal block is arranged at the lower end of the hollow pipe, a water seal groove is formed in the upper surface of the water seal block, the lower end of the hollow pipe is fixedly connected with the bottom inner wall of the water seal groove, a communication hole is formed in the lower end of the inner side wall of the hollow pipe, an annular plate is sleeved on the outer side of the lower end of the hollow pipe and fixedly connected with the bottom inner wall of the water seal groove, flow guide holes are formed in the periphery of the bottom inner wall of the water seal groove, an annular flow guide plate is fixedly connected to the edge of the lower, the upper end of the guide rod is positioned in the inner cavity of the annular guide plate, and the upper end surface of the guide rod is in fit connection with the lower end surface of the water seal block.
Preferably, the communication holes are provided in plurality, and the communication holes are distributed in an annular array.
Preferably, the flow guide holes are arranged in a plurality, and the flow guide holes are distributed in an annular array.
Preferably, the lateral wall of water seal piece and the lateral wall of annular guide plate all incline to set up, a plurality of deceleration tanks that are equidistant vertical distribution are seted up to the lateral wall annular of water conservancy diversion pole.
Preferably, the upper end of the hollow tube is fixedly connected with a funnel, and a plug is arranged at an opening at the upper end of the funnel.
Preferably, one end of the outer side wall of the culture bottle is communicated with a liquid outlet pipe, and a ball valve is arranged in the middle of the liquid outlet pipe.
The utility model discloses a technological effect and advantage:
1. the utility model discloses a set up the water seal groove on the upper surface of water seal block, the bottom inner wall of water seal groove and the lower extreme fixed connection of hollow tube, the inside wall lower extreme of hollow tube has seted up the intercommunicating pore, the annular plate has been cup jointed in the lower extreme outside of hollow tube, the nutrient solution that the annular plate inner chamber was stored can play the water seal effect to the inner chamber of intercommunicating pore to avoid the outside air to get into the inner chamber of blake bottle, and then avoided the stem cell to receive the pollution of bacterium in the air;
2. the utility model discloses a fixed surface is connected with annular guide plate under the water seal piece, and annular guide plate slope sets up, and the deceleration tank has been seted up to the lateral wall of water conservancy diversion pole, and the nutrient solution flows along the lateral wall of water conservancy diversion pole under annular guide plate guide effect, and the deceleration tank can slow down the flow of nutrient solution to avoid the too big impact stem cell of the nutrient solution velocity of flow and lead to the stem cell impaired.
Drawings
Fig. 1 is a schematic view of the overall structure section of the present invention.
Fig. 2 is a schematic sectional view of the water seal block of the present invention.
Fig. 3 is the utility model discloses hollow tube structure cuts open the schematic diagram that cuts open in bow.
In the figure: 1. a culture bottle; 2. an end cap; 3. a hollow tube; 4. a water sealing block; 5. water sealing the tank; 6. a communicating hole; 7. an annular plate; 8. a flow guide hole; 9. an annular baffle; 10. a guide rod; 11. a deceleration tank; 12. a funnel; 13. a plug; 14. a liquid outlet pipe; 15. a ball valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a water seal anti-pollution stem cell amplification device as shown in figures 1-3, as shown in figure 1, comprising a culture bottle 1, an end cover 2 is arranged at the upper end of the culture bottle 1, the end cover 2 is fixedly connected with the culture bottle 1 through screw threads, a sealing gasket is arranged between the end cover 2 and the culture bottle 1 and used for improving the sealing property between the end cover 2 and the culture bottle 1, a hollow pipe 3 is connected in the middle of the end cover 2 in a penetrating way, the hollow pipe 3 is fixedly connected with the end cover 2, a water sealing block 4 is arranged at the lower end of the hollow pipe 3, a funnel 12 is fixedly connected at the upper end of the hollow pipe 3, a plug 13 is arranged at the upper end opening of the funnel 12 and used for plugging the funnel 12, a liquid outlet pipe 14 is communicated with one end of the outer side wall of the culture bottle 1, a ball valve 15 is arranged at the middle part of the liquid outlet pipe, ball valve 15 is used to control the opening and closing of outlet pipe 14.
As shown in fig. 2 and 3, a water seal groove 5 is formed on the upper surface of a water seal block 4, the lower end of a hollow tube 3 is fixedly connected with the bottom end inner wall of the water seal groove 5, a plurality of communication holes 6 are formed in the lower end of the inner side wall of the hollow tube 3, the communication holes 6 are distributed in an annular array, the communication holes 6 are used for communicating the inner cavity of the hollow tube 3 with the water seal groove 5, an annular plate 7 is sleeved on the outer side of the lower end of the hollow tube 3, the annular plate 7 is fixedly connected with the bottom end inner wall of the water seal groove 5, the inner cavity of the hollow tube 3, the communication holes 6 and the inner cavity of the annular plate 7 form a communicating vessel, so that nutrient solution in the inner cavity of the annular plate 7 can form water seal to the communication holes 6, even if the upper end of the hollow tube 3 is communicated with the outside, bacteria in the outside air can not enter the inner cavity of the culture bottle 1, thereby effectively preventing, the plurality of guide holes 8 are arranged, the plurality of guide holes 8 are distributed in an annular array, the lower end face edge of the water sealing block 4 is fixedly connected with an annular guide plate 9, the outer side wall of the water sealing block 4 and the outer side wall of the annular guide plate 9 are obliquely arranged, the middle part of the lower end inner wall of the culture bottle 1 is fixedly connected with a guide rod 10, the upper end of the guide rod 10 is positioned in the inner cavity of the annular guide plate 9, the upper end face of the guide rod 10 is in fit connection with the lower end face of the water sealing block 4, nutrient solution in the inner cavity of the water sealing groove 5 drips through the guide holes 8, the annular guide plate 9 plays a guide role in guiding the nutrient solution, so that the nutrient solution dripping from the inner cavity of the guide holes 8 can flow along the outer side wall of the guide rod 10, the outer side wall of the guide rod 10 is annularly provided with a plurality of deceleration grooves 11 which are vertically distributed at, thereby avoiding the stem cells from being damaged due to the impact of the nutrient solution on the stem cells.
The utility model discloses the theory of operation:
when the device is used, the plug 13 is opened, nutrient solution is added into the hollow tube 3 from the funnel 12, the nutrient solution enters the inner cavity of the annular plate 7 through the communicating hole 6, when the nutrient solution overflows the upper end of the annular plate 7, the nutrient solution drips from the inner cavity of the water seal groove 5 through the guide hole 8, and the annular guide plate 9 guides the nutrient solution dripped in the guide hole 8, so that the nutrient solution flows along the outer side wall of the guide rod 10;
the reduction tank 11 can buffer and slow down the nutrient solution that the water conservancy diversion pole 10 lateral wall flows to avoided the too fast stem cell of assaulting the culture flask 1 inner chamber of nutrient solution velocity of flow and leaded to the stem cell impaired.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (6)
1. The utility model provides an anti-pollution stem cell amplification device of water seal, includes blake bottle (1), its characterized in that: the upper end of the culture bottle (1) is provided with an end cover (2), the end cover (2) is fixedly connected with the culture bottle (1) through threads, a sealing gasket is arranged between the end cover (2) and the culture bottle (1), the middle part of the end cover (2) is connected with a hollow pipe (3) in a penetrating way, the hollow pipe (3) is fixedly connected with the end cover (2), the lower end of the hollow pipe (3) is provided with a water sealing block (4), a water sealing groove (5) is formed in the upper surface of the water sealing block (4), the lower end of the hollow pipe (3) is fixedly connected with the bottom inner wall of the water sealing groove (5), a communication hole (6) is formed in the lower end of the inner side wall of the hollow pipe (3), an annular plate (7) is sleeved on the outer side of the lower end of the hollow pipe (3), the annular plate (7) is fixedly connected with the bottom inner wall of the water sealing groove (5), and a flow, the lower terminal surface edge fixedly connected with annular guide plate (9) of water seal piece (4), the lower extreme inner wall middle part fixedly connected with water conservancy diversion pole (10) of blake bottle (1), the upper end of water conservancy diversion pole (10) is located the inner chamber of annular guide plate (9), the up end of water conservancy diversion pole (10) and the lower terminal surface laminating of water seal piece (4) are connected.
2. The water-sealed anti-pollution stem cell expansion device according to claim 1, wherein: the communication holes (6) are arranged in a plurality, and the communication holes (6) are distributed in an annular array.
3. The water-sealed anti-pollution stem cell expansion device according to claim 2, wherein: the flow guide holes (8) are arranged in a plurality, and the flow guide holes (8) are distributed in an annular array.
4. The water-sealed anti-pollution stem cell expansion device according to claim 3, wherein: the lateral wall of water seal block (4) and the lateral wall of annular guide plate (9) all incline to set up, a plurality of deceleration grooves (11) that are equidistant vertical distribution are seted up to the lateral wall annular of water conservancy diversion pole (10).
5. The water-sealed anti-pollution stem cell expansion device according to claim 4, wherein: the upper end fixedly connected with funnel (12) of hollow tube (3), the upper end opening part of funnel (12) is provided with end cap (13).
6. The water-sealed anti-pollution stem cell expansion device according to claim 5, wherein: one end of the outer side wall of the culture bottle (1) is communicated with a liquid outlet pipe (14), and a ball valve (15) is arranged in the middle of the liquid outlet pipe (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022109121.3U CN213357615U (en) | 2020-09-23 | 2020-09-23 | Water-sealed anti-pollution stem cell amplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022109121.3U CN213357615U (en) | 2020-09-23 | 2020-09-23 | Water-sealed anti-pollution stem cell amplification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213357615U true CN213357615U (en) | 2021-06-04 |
Family
ID=76154881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022109121.3U Active CN213357615U (en) | 2020-09-23 | 2020-09-23 | Water-sealed anti-pollution stem cell amplification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213357615U (en) |
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2020
- 2020-09-23 CN CN202022109121.3U patent/CN213357615U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A water sealed anti pollution stem cell amplification device Effective date of registration: 20211224 Granted publication date: 20210604 Pledgee: Bohai Bank Co.,Ltd. Haikou Branch Pledgor: Hainan Susheng Biotechnology Co.,Ltd. Registration number: Y2021980016373 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231009 Granted publication date: 20210604 Pledgee: Bohai Bank Co.,Ltd. Haikou Branch Pledgor: Hainan Susheng Biotechnology Co.,Ltd. Registration number: Y2021980016373 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A water sealed and anti pollution stem cell amplification device Effective date of registration: 20240102 Granted publication date: 20210604 Pledgee: CITIC Bank Haikou branch Pledgor: Hainan Susheng Biotechnology Co.,Ltd. Registration number: Y2023980075689 |