CN210596028U

CN210596028U - Automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium

Info

Publication number: CN210596028U
Application number: CN201921211804.0U
Authority: CN
Inventors: 宋丽霞; 马立军; 阿古耶赫; 刘伟; 杨慧
Original assignee: Inner Mongolia Yinhong Stem Cell Life Science And Technology Investment Co ltd
Current assignee: Inner Mongolia Yinhong Stem Cell Life Science And Technology Investment Co ltd
Priority date: 2019-07-29
Filing date: 2019-07-29
Publication date: 2020-05-22
Anticipated expiration: 2029-07-29

Abstract

The utility model discloses an automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium, it includes nitrogen gas storage tank, filter, goes up level sensor, lower level sensor, pressure sensor, alarm device and controller. Has the advantages that: the utility model has simple structure, easy realization and automatic control; the pollution to the filter and the workbench is avoided, the waste of culture medium is avoided, and the cost for culturing stem cells is reduced; the amount of the culture medium in each culture bottle can be accurately controlled, and the effect of culturing stem cells is better; the filtering efficiency is improved, and the filtering cost is reduced; further ensuring better effect of stem cell culture.

Description

Automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium

The technical field is as follows:

the utility model relates to an automatic filling system, in particular to an automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium.

Background art:

since the twenty-first century, the important role of cell therapy in clinical therapy has become increasingly clear, and in particular stem cells are more considered to be ideal seed cells for cell therapy and tissue engineering due to their self-renewing, multipotent differentiation potential. For example, umbilical cord mesenchymal stem cells can be used for treating autoimmune diseases and regenerative medicine, however, the source of the stem cells is limited, the culture operation is complex, and the culture effect is influenced by slight change of the growth environment; in the process of cell culture, it is a daily work to fill a large amount of cell culture bottles with culture media respectively; at present, a culture medium is added into a cell culture bottle, the culture medium is poured into a filter firstly, then nitrogen is introduced into the filter, the filtration is carried out under the action of gas pressure, the filtered culture medium is conveyed into the culture bottle from a liquid outlet rubber tube at the bottom of the filter, when the culture medium added into the previous culture bottle is changed into the next culture bottle, the liquid outlet rubber tube needs to be pinched by hand, then a liquid outlet of the liquid outlet rubber tube is placed into the next culture bottle, the pinched liquid outlet rubber tube is loosened to add the culture medium, and the process is repeated until each culture bottle needed to be used is added; the following problems exist with the above approach: 1. because the shell of the filter is made of opaque stainless steel and the liquid inlet of the shell is small, the change of the liquid level in the filter cannot be seen clearly when the culture medium is poured into the filter from the liquid inlet every time, the culture medium is easy to overflow from the liquid inlet, the outer wall of the filter and a workbench are polluted, the culture medium is wasted, and the cost for culturing stem cells is increased; 2. the manual pinching mode is adopted, so that the situations that the culture medium leaks and wastes and pollutes a workbench easily occur when culture bottles are replaced, and the effect of culturing stem cells can be influenced because the amount of the culture medium in each culture bottle cannot be accurately controlled manually; 3. the existing filter is only provided with a filter disc on a support disc, in order to ensure the filtering effect, three layers of 0.22 mu m filter membranes are paved on the filter disc, and the laying of 3 layers of 0.22 mu m filter membranes ensures the filtering effect, but reduces the filtering efficiency and increases the filtering cost; 4. the existing filter is covered with a shell above the filter membrane, a sealing ring is arranged at the contact position of the shell and the filter membrane, the shell and a fixed disc are fixed through bolts, when the bolts are loosened, a culture medium can flow out from a gap between a sealing strip and the filter membrane, so that the waste of the culture medium and the pollution of a workbench are caused; when the bolt was screwed up when tighter, the sealing strip can crush the filter membrane, can lead to the bacterium in the culture medium can't be filtered will flow to the blake bottle in, and then lead to the effect variation of cultivateing stem cell.

The utility model has the following contents:

an object of the utility model is to provide a simple structure has improved the filter effect, and has realized automatic control's an automatic filling system that is used for preparing umbilical cord mesenchymal stem cell culture medium.

The utility model discloses by following technical scheme implement: an automatic filling system for preparing an umbilical cord mesenchymal stem cell culture medium comprises a nitrogen storage tank, a filter, an upper liquid level sensor, a lower liquid level sensor, a pressure sensor, an alarm device and a controller; the air outlet of the nitrogen storage tank is communicated with the air inlet of the filter through a pipeline; an electromagnetic valve is arranged on a pipeline between the nitrogen storage tank and the filter; the upper liquid level sensor and the lower liquid level sensor are respectively arranged above and below the inner wall of the shell of the filter, and the pressure sensor is arranged on the inner wall of the shell; a liquid outlet pipe is arranged at the liquid outlet of the filter, and a control valve is arranged on the liquid outlet pipe; the signal output ends of the upper liquid level sensor, the lower liquid level sensor and the pressure sensor are connected with the signal input end of the controller through signals; and the signal output end of the controller is respectively connected with the signal input ends of the alarm device, the electromagnetic valve and the control valve through signals.

Further, the filter comprises a supporting plate, at least 3 supporting legs, a first filter disc, the shell and a limiting ring; at least 3 support legs are fixed at the bottom of the support plate, the first filter plate which is coaxially arranged is placed above the support plate, the edge of the first filter plate is sealed with the support plate through a first seal ring, a first groove is formed in one surface of the support plate, which corresponds to the first filter plate, and a liquid outlet is formed in the closed surface of the first groove; a plurality of liquid outlet holes are formed in the first filter disc; a first filter membrane is laid above the first filter disc; the shell is covered above the first filter membrane, the top of the shell is respectively provided with the air inlet and the liquid inlet, the liquid inlet is covered with the sealing cover, and the edge of the bottom of the shell is provided with a plurality of bolts which are arranged along the circumferential direction of the shell and fixedly connected with the supporting disk; a second sealing ring is arranged at the contact position of the shell and the first filter membrane; a limiting ring is fixedly arranged at the bottom of the shell outside the second sealing ring; the bottom of the limiting ring is in contact with the supporting disc.

Further, the pore size of the first filter membrane is 0.22 μm.

Further, a second filter disc is arranged between the first filter membrane and the shell; the edge of the second filter disc is sealed with the first filter membrane through a third seal ring, a second groove is formed in the second filter disc on the inner side of the third seal ring, and the opening surface of the second groove is opposite to the first filter membrane; the closed end of the second groove is provided with a plurality of through holes; a second filter membrane is laid on one surface of the second filter disc opposite to the shell; the second sealing ring is arranged at the contact position of the shell and the second filter membrane.

Further, the pore size of the second filter membrane is 0.45 μm.

The utility model has the advantages that: 1. the utility model has simple structure, easy realization and automatic control; when the liquid level of the culture medium poured into the filter reaches the upper liquid level sensor, the upper liquid level sensor sends a signal to the controller, the controller controls the alarm device to give an alarm, and the worker stops pouring the culture medium into the filter, so that the phenomenon that the culture medium overflows from the liquid inlet due to unclear liquid level is avoided, the filter and a workbench are prevented from being polluted, the waste of the culture medium is avoided, and the cost for culturing the dry cells is reduced; 2. the controller controls the control valve to open and close at fixed time, so that the situations of medium leakage and waste and workbench pollution are avoided, the amount of the medium in each culture bottle can be accurately controlled, and the effect of culturing stem cells is better; 3. the second filter membrane of 0.45 mu m is paved on the second filter disc above the first filter membrane of 0.22 mu m, thereby ensuring the filtering effect, improving the filtering efficiency and reducing the filtering cost. 4. The bottom of the shell is additionally provided with the limiting ring, the height of the limiting ring is the distance between the bottom of the shell and the supporting disk when the bolt is screwed right (namely, the culture medium is not leaked, and the first filter membrane or the second filter membrane is not crushed), so that the second filter membrane and the first filter membrane are effectively guaranteed to be just pressed tightly by the second sealing ring and the third sealing ring, the situation that the bolt is screwed loose and the sealing performance is poor is avoided, and the situation that the bolt is screwed tight and the first filter membrane or/and the second filter membrane are crushed is also avoided; and then improved the installation effectiveness of filter, guaranteed the filtration quality of culture medium simultaneously, further guaranteed the effect of cultivateing stem cell preferred.

Description of the drawings:

in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic view of the overall structure of the embodiment of the present invention.

Fig. 2 is a partially enlarged schematic view of a portion a in fig. 1.

The device comprises a nitrogen storage tank 1, a filter 2, a supporting plate 201, a first groove 201.1, a liquid outlet 201.2, a supporting leg 202, a first filter disc 203, a liquid outlet hole 203.1, a shell 204, an air inlet 204.1, a liquid inlet 204.2, a limiting ring 205, a first sealing ring 206, a first filter membrane 207, a sealing cover 208, a bolt 209, a second filter disc 210, a second groove 210.1, a through hole 210.2, a third sealing ring 211, a second filter membrane 212, a second sealing ring 213, an upper liquid level sensor 3, a lower liquid level sensor 4, a pressure sensor 5, an alarm device 6, a controller 7, an electromagnetic valve 8, a liquid outlet pipe 9 and a control valve 10.

The specific implementation mode is as follows:

as shown in fig. 1-2, an automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium comprises a nitrogen storage tank 1, a filter 2, an upper liquid level sensor 3, a lower liquid level sensor 4, a pressure sensor 5, an alarm device 6 and a controller 7; the air outlet of the nitrogen storage tank 1 is communicated with the air inlet 204.1 of the filter 2 through a pipeline; an electromagnetic valve 8 is arranged on a pipeline between the nitrogen storage tank 1 and the filter 2; an upper liquid level sensor 3 and a lower liquid level sensor 4 are respectively arranged above and below the inner wall of the shell 204 of the filter 2, and a pressure sensor 5 is arranged on the inner wall of the shell 204; a liquid outlet pipe 9 is arranged at the liquid outlet 201.2 of the filter 2, and a control valve 10 is arranged on the liquid outlet pipe 9; the signal output ends of the upper liquid level sensor 3, the lower liquid level sensor 4 and the pressure sensor 5 are connected with the signal input end of the controller 7 through signals; the signal output ends of the controller 7 are respectively connected with the signal input ends of the alarm device 6, the electromagnetic valve 8 and the control valve 10 through signals; the upper liquid level sensor 3, the lower liquid level sensor 4, the pressure sensor 5, the controller 7 and the alarm device 6 are all conventional devices.

The filter 2 comprises a support plate 201, at least 3 support legs 202, a first filter plate 203, a shell 204 and a limit ring 205; at least 3 supporting legs 202 are fixed at the bottom of a supporting plate 201, a first filter disc 203 which is coaxially arranged is placed above the supporting plate 201, the edge of the first filter disc 203 is sealed with the supporting plate 201 through a first sealing ring 206, a first groove 201.1 is formed in one surface, corresponding to the first filter disc 203, of the supporting plate 201, and a liquid outlet 201.2 is formed in the closed surface of the first groove 201.1; a plurality of liquid outlet holes 203.1 are arranged on the first filter disc 203; a first filter membrane 207 is laid above the first filter disc 203, and the aperture of the first filter membrane 207 is 0.22 μm; a shell 204 is covered above the first filter membrane 207, the top of the shell 204 is respectively provided with an air inlet 204.1 and a liquid inlet 204.2, the liquid inlet 204.2 is covered with a sealing cover 208, and the bottom edge of the shell 204 is provided with a plurality of bolts 209 which are fixedly connected with the support plate 201 and are arranged along the circumferential direction; a second filter disc 210 is arranged between the first filter membrane 207 and the shell 204; the edge of the second filter disc 210 is sealed with the first filter membrane 207 through a third sealing ring 211, a second groove 210.1 is formed in the second filter disc 210 on the inner side of the third sealing ring 211, and the opening surface of the second groove 210.1 is opposite to the first filter membrane 207; the closed end of the second groove 210.1 is provided with a plurality of through holes 210.2; a second filter membrane 212 is laid on one surface of the second filter disc 210 opposite to the shell 204, and the aperture of the second filter membrane 212 is 0.45 μm, so that the filter effect is ensured, the filter efficiency is improved, and the filter cost is reduced; a second sealing ring 213 is arranged at the contact part of the shell 204 and the second filter membrane 212; a limiting ring 205 is fixedly arranged at the bottom of the shell 204 outside the second sealing ring 213; the bottom of the limiting ring 205 is in contact with the support plate 201; the height of the limiting ring 205 is the distance between the bottom of the shell 204 and the support plate 201 when the bolt 209 is screwed right (i.e. the culture medium is not leaked, and the first filter membrane 207 or the second filter membrane 212 is not crushed), so that the second seal ring 213 and the third seal ring 211 are effectively ensured to just compress the second filter membrane 212 and the first filter membrane 207, and the first filter membrane 207 or/and the second filter membrane 212 cannot be crushed when the bolt 209 is screwed loose or the bolt 209 is screwed tight; and then improved the installation effectiveness of filter 2, guaranteed the filtration quality of culture medium simultaneously, further guaranteed the effect of cultivateing stem cell preferred.

The working process is as follows: the culture medium is poured into the filter 2 manually, when the liquid level of the culture medium poured into the filter 2 reaches the upper liquid level sensor 3, the upper liquid level sensor 3 sends a signal to the controller 7, the controller 7 controls the alarm device 6 to give an alarm, and workers stop pouring the culture medium into the filter 2, so that the phenomenon that the culture medium overflows from the liquid inlet 204.2 due to unclear liquid level is avoided, the filter 2 and a workbench are prevented from being polluted, the culture medium is prevented from being wasted, and the cost for culturing dry cells is reduced; then the alarm device 6 is closed, the sealing cover 208 is covered on the liquid inlet 204.2 of the filter 2, the electromagnetic valve 8 is opened through the control controller 7, nitrogen in the nitrogen storage tank 1 is introduced into the filter 2, the pressure sensor 5 transmits a detected pressure value signal to the controller 7 at any time, when the detected signal reaches a signal set on the controller 7, the controller 7 controls the control valve 10 to be opened, the culture medium sequentially passes through the second filter membrane 212 and the first filter membrane 207 under the action of gas pressure, and finally is discharged into the culture bottle from the liquid outlet pipe 9; after a certain time interval, the controller 7 controls the control valve 10 to close, then the time is delayed for 2-3s, the outlet of the liquid outlet pipe 9 is placed into a new culture bottle, after the time is delayed for 2-3s, the controller 7 controls the control valve 10 to open again, and the culture medium is added into the corresponding culture bottle again; the situations of medium leakage and waste and workbench pollution are avoided, the amount of the medium in each culture bottle can be accurately controlled, and the good effect of culturing stem cells is ensured; when the liquid level of the culture medium in the filter 2 is reduced below the lower liquid level sensor 4, the lower liquid level sensor 4 sends a signal to the controller 7, the controller 7 controls the electromagnetic valve 8 to be closed, and after a certain time delay, the controller 7 controls the valve 10 to be closed, so that the pressure relief in the filter 2 is realized; the utility model discloses simple structure easily realizes to automated control has been realized.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An automatic filling system for preparing an umbilical cord mesenchymal stem cell culture medium is characterized by comprising a nitrogen storage tank, a filter, an upper liquid level sensor, a lower liquid level sensor, a pressure sensor, an alarm device and a controller; the air outlet of the nitrogen storage tank is communicated with the air inlet of the filter through a pipeline; an electromagnetic valve is arranged on a pipeline between the nitrogen storage tank and the filter; the upper liquid level sensor and the lower liquid level sensor are respectively arranged above and below the inner wall of the shell of the filter, and the pressure sensor is arranged on the inner wall of the shell; a liquid outlet pipe is arranged at the liquid outlet of the filter, and a control valve is arranged on the liquid outlet pipe; the signal output ends of the upper liquid level sensor, the lower liquid level sensor and the pressure sensor are connected with the signal input end of the controller through signals; and the signal output end of the controller is respectively connected with the signal input ends of the alarm device, the electromagnetic valve and the control valve through signals.

2. The automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium of claim 1, wherein the filter comprises a support plate, at least 3 support legs, a first filter plate, the housing and a stop collar; at least 3 support legs are fixed at the bottom of the support plate, the first filter plate which is coaxially arranged is placed above the support plate, the edge of the first filter plate is sealed with the support plate through a first seal ring, a first groove is formed in one surface of the support plate, which corresponds to the first filter plate, and a liquid outlet is formed in the closed surface of the first groove; a plurality of liquid outlet holes are formed in the first filter disc; a first filter membrane is laid above the first filter disc; the shell is covered above the first filter membrane, the top of the shell is respectively provided with the air inlet and the liquid inlet, the liquid inlet is covered with the sealing cover, and the edge of the bottom of the shell is provided with a plurality of bolts which are arranged along the circumferential direction of the shell and fixedly connected with the supporting disk; a second sealing ring is arranged at the contact position of the shell and the first filter membrane; a limiting ring is fixedly arranged at the bottom of the shell outside the second sealing ring; the bottom of the limiting ring is in contact with the supporting disc.

3. The automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium of claim 2, wherein the pore size of the first filter is 0.22 μm.

4. The automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium according to claim 2 or 3, characterized in that a second filter disc is arranged between the first filter membrane and the shell; the edge of the second filter disc is sealed with the first filter membrane through a third seal ring, a second groove is formed in the second filter disc on the inner side of the third seal ring, and the opening surface of the second groove is opposite to the first filter membrane; the closed end of the second groove is provided with a plurality of through holes; a second filter membrane is laid on one surface of the second filter disc opposite to the shell; the second sealing ring is arranged at the contact position of the shell and the second filter membrane.

5. The automatic filling system for preparing umbilical cord mesenchymal stem cell culture medium of claim 4, wherein the pore size of the second filter is 0.45 μm.