CN111530285A - Filtering and collecting device suitable for large-scale production of biological products - Google Patents
Filtering and collecting device suitable for large-scale production of biological products Download PDFInfo
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- CN111530285A CN111530285A CN202010451960.5A CN202010451960A CN111530285A CN 111530285 A CN111530285 A CN 111530285A CN 202010451960 A CN202010451960 A CN 202010451960A CN 111530285 A CN111530285 A CN 111530285A
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- tank body
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
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Abstract
The invention discloses a filtering and collecting device suitable for large-scale production of biological products, which comprises an upper tank body and a lower tank body, wherein the upper tank body is connected with the lower tank body through a filtering and supporting structure, the left side and the right side of a waste liquid port are respectively provided with a first standby port and a culture medium filling port, two liquid level sensors are arranged in the upper tank body, the left side and the right side of a cell liquid outlet are respectively provided with a second standby port and a cell liquid inlet, the upper tank body is internally provided with a first sealing part, the lower tank body is internally provided with a second sealing part, a first filter membrane supporting structure is arranged in the first sealing part, a second filter membrane supporting structure is arranged in the second sealing part, a unique replacement filter membrane structure is adopted, pressure filtering is applied, a flushing process ensures high-efficiency replacement rate and high cell harvesting rate and reduces cell damage in the production process, and the special sealing and supporting structure ensures; the modular design, with low costs, do benefit to the batch replication, production.
Description
Technical Field
The invention relates to the technical field of filtering and collecting devices, in particular to a filtering and collecting device suitable for large-scale production of biological products.
Background
In the bioreactor production process, the filtration of waste liquid, the replacement of culture medium, and the enrichment and harvest of cell products are key links. Efficient waste filtration, media replacement, cell enrichment and harvesting, and material safety are critical to bioreactor system performance. The traditional ATF enrichment module has the defects of repeated use, high cost, cell enrichment and low harvesting efficiency.
Disclosure of Invention
The invention aims to provide a filtering and collecting device suitable for large-scale production of biological products, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the filtering and collecting device comprises an upper tank body and a lower tank body, wherein the upper tank body is connected with the lower tank body through a filtering and supporting structure, a waste liquid port is formed in the center of the top end of the upper tank body, a first standby port and a culture medium filling port are formed in the left side and the right side of the waste liquid port respectively, two liquid level sensors are arranged in the upper tank body, a cell liquid outlet is formed in the center of the bottom of the lower tank body, a second standby port and a cell liquid inlet are formed in the left side and the right side of the cell liquid outlet respectively, a first sealing part is formed in the upper tank body, a second sealing part is formed in the lower tank body, a first filter membrane supporting structure is arranged in the first sealing part, and a second filter membrane supporting structure is arranged in the second sealing part.
Further, the upper tank body and the lower tank body are sealed by the connecting flanges of the upper tank body and the lower tank body,
furthermore, the upper tank body and the lower tank body are sealed and made of biocompatible materials.
Furthermore, two level sensor is upper and lower setting in last jar of body.
Furthermore, the first filter membrane support structure and the second filter membrane support structure are both located at the filtering and support structure.
Further, the whole filtering and collecting device adopts GMP compliant materials.
The invention has the beneficial effects that: the upper tank body structure and the lower tank body structure are connected through flanges, so that the modular production and installation are facilitated;
the upper tank body and the lower tank body are sealed by sealing and made of biocompatible materials, so that the safety is ensured;
the filter membrane with proper material and pore size is selected, so that the filtration and recovery efficiency is effectively ensured, and the filter membrane support structure ensures that the filter membrane is not damaged by pressure;
in the cleaning process of the cell filter membrane, a culture medium is pumped into a culture medium filling port, a cell sap outlet sucks cell sap, the pressure of an upper tank body and a lower tank body is adjusted to ensure the cleaning effect, and two liquid level sensors are arranged on the tank bodies and used for monitoring the height limit of a liquid level to ensure the safety of the system;
in the cell filtering, replacing and harvesting processes, cells are pumped into a cell fluid inlet and a cell fluid outlet, a culture medium is pumped into a culture medium filling port, a waste liquid outlet discharges waste liquid, the filtration, replacement and harvesting of cell fluid are realized, the speed and the efficiency are ensured by adjusting the pressure of an upper tank body and a lower tank body simultaneously, and two liquid level sensors are arranged on the tank body and used for monitoring the height limit of a liquid level to ensure the safety of a system.
Drawings
FIG. 1 is a front view of a filtration and collection device suitable for large-scale production of biological products according to the present invention;
FIG. 2 is a top view of a modular flange connection design of a filtration and collection device suitable for large-scale production of biological products according to the present invention;
FIG. 3 is a diagram of the filtering support structure of the upper tank of the filtering and collecting device suitable for mass production of biological products according to the present invention;
fig. 4 is a structure diagram of a lower tank body filtering support structure of the filtering and collecting device suitable for large-scale production of biological products.
The reference numerals are explained below:
in the figure: 1. feeding the tank body; 2. a tank body is arranged; 3. a filtration and support structure; 4. a cell sap inlet; 5. a cell sap outlet; 6. a second spare port; 7. a liquid level sensor; 8. a first spare port; 9. a waste liquid port; 10. a culture medium filling port; 11. the upper tank body and the lower tank body are connected with flanges; 12. a first seal; 13. a first filter membrane support structure; 14. a second seal; 15. second filter membrane supporting structure
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Several embodiments of the invention are presented in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-4, the present invention provides a technical solution:
a filtering and collecting device suitable for large-scale production of biological products, which comprises an upper tank body 1 and a lower tank body 2, the upper tank body 1 and the lower tank body 2 are connected through a filtering and supporting structure 3, a waste liquid port 9 is arranged at the center of the top end of the upper tank body 1, the left side and the right side of the waste liquid port 9 are respectively provided with a first standby port 8 and a culture medium filling port 10, two liquid level sensors 7 are arranged in the upper tank body 1, a cell sap outlet 5 is arranged at the center of the bottom of the lower tank body 2, the left side and the right side of the cell sap outlet 5 are respectively provided with a second standby port 6 and a cell sap inlet 4, a first sealing position 12 is arranged in the upper tank body 1, a second sealing position 14 is arranged in the lower tank body 2, a first filter membrane supporting structure 13 is arranged inside the first sealing part 12, and a second filter membrane supporting structure 14 is arranged inside the second sealing part 14.
In order to further improve the use function of the filtering and collecting device suitable for the scale production of biological products, the upper tank body 1 and the lower tank body 2 are sealed by the upper tank body connecting flange 11 and the lower tank body connecting flange 11,
in order to further improve the use function of the filtering and collecting device suitable for large-scale production of biological products, the upper tank body 1 and the lower tank body 2 are sealed and made of biocompatible materials.
In order to further improve the use function of the filtering and collecting device suitable for large-scale production of biological products, the two liquid level sensors 7 are arranged in the upper tank body 1 from top to bottom.
In order to further improve the function of the filtration and collection device suitable for large-scale production of biological products, the first filter membrane support structure 14 and the second filter membrane support structure 15 are both located at the filtration and support structure 3.
In order to further improve the use function of the filtering and collecting device suitable for large-scale production of biological products, the filtering and collecting device integrally adopts GMP (good manufacturing practice) compliant materials.
The working process is as follows:
from the cytosol inlet, the cell solution to be filtered or enriched is added to the upper sensor level. The waste liquid enters a tank waste liquid storage area on the filtering membrane, and the effective cell product solution is intercepted by the filtering membrane in a tank product collection area below the filtering membrane; the waste liquid in the culture process is discharged into a waste liquid tank through an upper waste liquid port. When the liquid level is lowered to the lower sensor, the liquid waste is stopped to be discharged, and a new culture medium is filled. Pumping new culture medium into the filtering/enriching unit to the upper sensor position to effectively resuspend the cell product; the cell culture solution and the newly supplemented culture medium pass through the cell sap outlet and reenter the cell culture main circulation.
In conclusion, the upper tank body structure and the lower tank body structure are connected through the flange, so that the modular production and installation are facilitated;
the upper tank body and the lower tank body are sealed by sealing and made of biocompatible materials, so that the safety is ensured;
the filter membrane with proper material and pore size is selected, so that the filtration and recovery efficiency is effectively ensured, and the filter membrane support structure ensures that the filter membrane is not damaged by pressure;
in the cleaning process of the cell filter membrane, a culture medium is pumped into a culture medium filling port, a cell sap outlet sucks cell sap, the pressure of an upper tank body and a lower tank body is adjusted to ensure the cleaning effect, and two liquid level sensors are arranged on the tank bodies and used for monitoring the height limit of a liquid level to ensure the safety of the system;
in the cell filtering, replacing and harvesting processes, cells are pumped into a cell fluid inlet and a cell fluid outlet, a culture medium is pumped into a culture medium filling port, a waste liquid outlet discharges waste liquid, the filtration, replacement and harvesting of cell fluid are realized, the speed and the efficiency are ensured by adjusting the pressure of an upper tank body and a lower tank body simultaneously, and two liquid level sensors are arranged on the tank body and used for monitoring the height limit of a liquid level to ensure the safety of a system.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. The utility model provides a filter collection device suitable for biological product scale production, includes jar body (1) and lower jar of body (2), its characterized in that: the upper tank body (1) and the lower tank body (2) are connected through a filtering and supporting structure (3), a waste liquid port (9) is arranged at the center of the top end of the upper tank body (1), a first standby port (8) and a culture medium filling port (10) are respectively arranged at the left side and the right side of the waste liquid port (9), two liquid level sensors (7) are arranged in the upper tank body (1), a cell sap outlet (5) is arranged at the center of the bottom of the lower tank body (2), the left side and the right side of the cell sap outlet (5) are respectively provided with a second standby port (6) and a cell sap inlet (4), a first sealing position (12) is arranged in the upper tank body (1), a second sealing position (14) is arranged in the lower tank body (2), a first filter membrane supporting structure (13) is arranged in the first sealing part (12), and a second filter membrane supporting structure (14) is arranged inside the second sealing part (14).
2. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the upper tank body (1) and the lower tank body (2) are sealed through an upper tank body connecting flange and a lower tank body connecting flange (11).
3. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the upper tank body (1) and the lower tank body (2) are sealed and made of biocompatible materials.
4. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the two liquid level sensors (7) are arranged in the upper tank body (1) from top to bottom.
5. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the first filter membrane support structure (14) and the second filter membrane support structure (15) are both located at the filtration and support structure (3).
6. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the whole filtering and collecting device adopts GMP compliant materials.
7. The filtration and collection device suitable for large-scale production of biological products according to claim 1, wherein: the first seal (12) and the second seal (14) are located at the filter support structure (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010451960.5A CN111530285A (en) | 2020-05-26 | 2020-05-26 | Filtering and collecting device suitable for large-scale production of biological products |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010451960.5A CN111530285A (en) | 2020-05-26 | 2020-05-26 | Filtering and collecting device suitable for large-scale production of biological products |
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| CN111530285A true CN111530285A (en) | 2020-08-14 |
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| CN202010451960.5A Pending CN111530285A (en) | 2020-05-26 | 2020-05-26 | Filtering and collecting device suitable for large-scale production of biological products |
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Application publication date: 20200814 |
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