CN206109422U - Living cells piece -rate system - Google Patents
Living cells piece -rate system Download PDFInfo
- Publication number
- CN206109422U CN206109422U CN201621074283.5U CN201621074283U CN206109422U CN 206109422 U CN206109422 U CN 206109422U CN 201621074283 U CN201621074283 U CN 201621074283U CN 206109422 U CN206109422 U CN 206109422U
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- China
- Prior art keywords
- container
- fluid pump
- living cells
- doughnut
- rate system
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Abstract
The utility model relates to a living cells piece -rate system, a living cells piece -rate system, including the reactor, hollow fiber slipstream filter, the booster, first container, the second container, first fluid pump and second fluid pump, the reactor passes through the water inlet intercommunication of booster and hollow fiber slipstream filter one end, the water inlet and the first container intercommunication of the hollow fiber slipstream filter other end, be through structure between first container and the second container, it promotes the formula barrier film to install fluid pressure between first container and the second container, first fluid pump and second fluid pump pass through pipe connection to second container. The utility model discloses the easy -to -use control of system stability reduces the contaminated risk of cell culture stoste, improves the separation efficiency of cell.
Description
Technical field
This utility model belongs to the detached technical field of biological cell, more particularly to a kind of living cells piece-rate system.
Background technology
During bio-pharmaceuticals, the ingredient in cell is in less, the most economical life of relative scale with high concentration
Cultivated in thing reactor.Now, the perfusion system for being used must have cell retention function, to cut as much as possible
Stay the useful medium in bioreactor.Preferably cell retention system should both have good perfusion ability, have again
There is very high cell retention ability, should not also affect the activity of cell during cell retention as much as possible, can be in most short stop
The cell of retention is fed back in cultivation reactor in time.
Generally there is the disadvantages such as stability of control system is not high, cell culture stock solution is contaminated in existing living cells piece-rate system
End, and the inefficiency of cell separation, reduce the output of cell pharmaceutical product.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of living cells piece-rate system, the easy-to-use control of system stability
System, reduces the contaminated risk of cell culture stock solution, improves the separation efficiency of cell.
This utility model solves the technical scheme that its technical problem adopted and is to provide a kind of living cells piece-rate system, including
Reactor, doughnut tangential flow filter, supercharger, the first container, second container, first fluid pump and second fluid pump,
The reactor is connected by supercharger with the water inlet of doughnut tangential flow filter one end, the doughnut slipstream
The water inlet of the filter other end and the first reservoir, are through structure between first container and second container, the
Fluid pressure driving isolating membrane is installed, the first fluid pump and second fluid pump pass through between one container and second container
Pipeline is connected to second container.
A kind of preferred embodiment as this utility model, the inside of the doughnut tangential flow filter is by thin
Born of the same parents' retention fibrous membrane forms cell culture stock solution circulation passage, and the cavity on the outside of the cell retention fibrous membrane is provided with some waste liquids
Outlet.
Used as this utility model another preferred embodiment, the doughnut tangential flow filter is vertically
Arrange, first container is located at the surface of second container, the isolating membrane is horizontally disposed in the first container and the
Between two containers.
Used as this utility model another preferred embodiment, the first fluid pump and second fluid pump pass through common
Pipeline connect with second container.
Beneficial effect
In this utility model, the power source of system is provided by fluid pump, can more the pressure of accuracy-control system,
The parameter such as time and flow, it is so as to realize the stability of system and easily controllable；System is controlled using liquid, is reduced
Isolation membrane damage inside container, reduces the contaminated risk of cell culture stock solution；By drawing liquid operating mode with pressurization operating mode it
Between change the time difference of presence, there is provided one instantaneous to filter inside and outside instantaneous differential pressure, then coordinates the pressure effect of supercharger, improves
Cell retention detached effect, so as to be conducive to improve production efficiency.
Description of the drawings
Fig. 1 system equipment attachment structure figures of the present utility model.
Specific embodiment
With reference to specific embodiment, this utility model is expanded on further.It should be understood that these embodiments are merely to illustrate this
Utility model rather than restriction scope of the present utility model.In addition, it is to be understood that in the content for having read this utility model instruction
Afterwards, those skilled in the art can make various changes or modifications to this utility model, and these equivalent form of values equally fall within this Shen
Please appended claims limited range.
A kind of living cells piece-rate system as shown in Figure 1, including reactor, doughnut tangential flow filter, supercharger,
First container, second container, first fluid pump and second fluid pump.
Doughnut tangential flow filter is vertically arranged, and reactor is tangentially flow through by supercharger with doughnut
The water inlet connection of filter upper end, the water inlet and the first reservoir of doughnut tangential flow filter lower end.Doughnut
The inside of tangential flow filter forms cell culture stock solution circulation passage by cell retention fibrous membrane, and cell culture stock solution is in Jing
Carry out cell while crossing the circulation passage to dam separation, the cavity on the outside of cell retention fibrous membrane is provided with some waste liquids and discharges
Mouthful, the waste liquid for filtering is discharged outside doughnut tangential flow filter.
First container is located at the surface of second container, is through structure between the first container and second container, and first
Fluid pressure driving isolating membrane is horizontally installed between container and second container.First fluid pump and second fluid pump are by pipe
Road is connected to second container, and first fluid pump and second fluid pump are connected by common pipeline with second container, reduces pipeline
Laying.
One kind is provided below carries out the detached method of living cells using above-mentioned living cells piece-rate system, comprises the following steps：
(1) drawing liquid operating mode：Start first fluid pump, drawing liquid, drawing liquid time are carried out to second container by first fluid pump
8～12s is controlled to, is advisable with being set to 10s.Isolating membrane compresses in the presence of second container negative pressure to second container, so as to
So that the first container forms negative pressure, the cell culture stock solution in reactor enters into first by doughnut tangential flow filter
In container, now supercharger is opened into atmospheric pressure state.
(2) pressurization operating mode：Terminate drawing liquid, stop first fluid pump, start second fluid pump, by second fluid pump to the
Two containers are pressurizeed, and pressing time is controlled to 2～4s, are advisable with being set to 3s.Effect of the isolating membrane in second container malleation
Lower to compress to the first container, the cell culture stock solution inside the first container is by doughnut tangential flow filter and in supercharging
It is back in the presence of device in reactor, now supercharger work so that doughnut tangential flow filter is to supercharger end shape
Into certain pressure.
(3) by first fluid pump and the mutual switch operating repeat step (1) of second fluid pump and step (2), cell is made
Culture stock solution iterates through doughnut tangential flow filter, and under the pressurization of conduit pressurizer, realizes that cell dams
Function is so as to reaching detached effect.
The system provides the power source of system by fluid pump, can the more pressure of accuracy-control system, time and stream
The parameters such as amount, it is so as to realize the stability of system and easily controllable.System is controlled using liquid, is reduced inside container
Isolation membrane damage, reduce the contaminated risk of cell culture stock solution.Deposited by changing between drawing liquid operating mode and pressurization operating mode
Time difference, there is provided one instantaneous to filter inside and outside instantaneous differential pressure, then coordinates the pressure effect of supercharger, improves cell and cuts
Detached effect is stayed, so as to be conducive to improve production efficiency.
Claims (4)
1. a kind of living cells piece-rate system, including reactor, doughnut tangential flow filter, supercharger, the first container, second
Container, first fluid pump and second fluid pump, it is characterised in that：The reactor is tangentially flow through by supercharger with doughnut
The water inlet connection of filter one end, the water inlet and the first reservoir of the doughnut tangential flow filter other end, institute
It is through structure to state between the first container and second container, and Fluid pressure promotion is provided between the first container and second container
Formula isolating membrane, the first fluid pump and second fluid pump are connected to second container by pipeline.
2. a kind of living cells piece-rate system according to claim 1, it is characterised in that：The doughnut tangential flow filtration
The inside of device forms cell culture stock solution circulation passage, the chamber on the outside of the cell retention fibrous membrane by cell retention fibrous membrane
Body is provided with some waste liquid outlets.
3. a kind of living cells piece-rate system according to claim 1, it is characterised in that：The doughnut tangential flow filtration
Device is vertically arranged, first container be located at second container surface, the isolating membrane it is horizontally disposed in
Between first container and second container.
4. a kind of living cells piece-rate system according to claim 1, it is characterised in that：The first fluid pump and second
Body pump is connected by common pipeline with second container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621074283.5U CN206109422U (en) | 2016-09-22 | 2016-09-22 | Living cells piece -rate system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621074283.5U CN206109422U (en) | 2016-09-22 | 2016-09-22 | Living cells piece -rate system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206109422U true CN206109422U (en) | 2017-04-19 |
Family
ID=58521589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621074283.5U Expired - Fee Related CN206109422U (en) | 2016-09-22 | 2016-09-22 | Living cells piece -rate system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206109422U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434341A (en) * | 2016-09-22 | 2017-02-22 | 上海拜高乐生物技术有限公司 | Living cell separating system and separating method adopting same |
-
2016
- 2016-09-22 CN CN201621074283.5U patent/CN206109422U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434341A (en) * | 2016-09-22 | 2017-02-22 | 上海拜高乐生物技术有限公司 | Living cell separating system and separating method adopting same |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170419 Termination date: 20210922 |