CN207944110U - A kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture - Google Patents
A kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture Download PDFInfo
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- CN207944110U CN207944110U CN201820138285.9U CN201820138285U CN207944110U CN 207944110 U CN207944110 U CN 207944110U CN 201820138285 U CN201820138285 U CN 201820138285U CN 207944110 U CN207944110 U CN 207944110U
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Abstract
A kind of ultrasonic sedimentation combination perfusion system for zooblast perfusion culture, including retort, cell separator, ultrasonic power controller, peristaltic pump, automatic controller and gas-liquid separator；The retort is connected by being equipped with the liquid suction pipe of peristaltic pump with culture medium；The gas-liquid separator is mounted in retort, and inlet is provided in the middle part of the gas-liquid separator；The bottom of gas-liquid separator is provided with liquid outlet, and liquid outlet is connected with retort；Outer circulation mouth is provided with below gas-liquid separator, outer circulation mouth is connected by liquid suction pipe with cell separator, cell separator is connected by being equipped with the liquid suction pipe of peristaltic pump with flow container is received, and the cell in gas-liquid separator in culture solution, which is drawn under the action of peristaltic pump in cell separator, carries out retention processing；The cell separator is connected to ultrasonic power controller signals, the peristaltic pump with automatic controller signal communication.The utility model can improve cell retention effect, improve production efficiency.
Description
Technical field
The utility model is related to field of cell culture, and in particular to a kind of ultrasound-for zooblast perfusion culture is heavy
Drop combines perfusion system.
Background technology
Kind with zooblast producer gene engineering drug is more and more, and the especially appearance of engineered antibody, yield is
As " bottleneck " of production.And ultrasound retention can be used as a kind of effective cell retention mode in currently available technology, but it is existing
There is the still ventilation culture of the cultural method generally use in technology, since still ventilating mode is limited to still snorkel and its set
Wound packages is set, and can not be further amplified so that project industrialization is limited, needs to solve by new ventilating mode and sedimentation device
Produce Industrialization, if but be passed through gas in culture, bubble can enter in the resonator in cell separator, can be tight
Ghost image rings cell retention effect so that the quality of production and efficiency cannot all improve.
Utility model content
In view of the deficiencies of the prior art, the purpose of the utility model is to provide a kind of for zooblast perfusion culture
Ultrasound-sedimentation combination perfusion system, on the one hand, can effectively avoid gas from entering in the resonator of cell separator, improve retention
Effect.On the other hand, by the settlement action of gas-liquid separator, part cell settlement can be detached, another part cell is with outer
It is recycled into cell separator, by ultrasonic Separation, the two combines and production efficiency is greatly improved.
To achieve the above object, the utility model adopts the following technical solution：
A kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture, including the separation of retort, cell
Device, ultrasonic power controller, peristaltic pump, automatic controller and gas-liquid separator；The retort is by being equipped with peristaltic pump
Liquid suction pipe is connected with culture medium；The gas-liquid separator is mounted in retort, is provided in the middle part of the gas-liquid separator
The inlet of inlet, gas-liquid separator is maintained under the liquid level of retort；The bottom of gas-liquid separator is provided with liquid outlet,
Liquid outlet is connected with retort；Outer circulation mouth is provided with below gas-liquid separator, outer circulation mouth passes through liquid suction pipe and cell
Separator is connected, and cell separator is connected by being equipped with the liquid suction pipe of peristaltic pump with flow container is received, and is trained in gas-liquid separator
Cell in nutrient solution, which is drawn under the action of peristaltic pump in cell separator, carries out retention processing；The cell separator with it is super
Acoustical power controller signals be connected to, the peristaltic pump with automatic controller signal communication.
Further, the cell separator offers circulation port, and the circulation port is by being equipped with the drawing liquid of peristaltic pump
It manages and is connected at the top of gas-liquid separator.
Further, switch folder, the switch folder and automatic controller are installed at the circulation port of the cell separator
It is connected.
Further, the cell separator is further opened with air inlet, is passed through cooling gas toward air inlet, is detached for cell
Device reduces operating temperature.
Further, blender, the blender and the motor at the top of retort are installed in the retort
It is connected, motor drives blender to be stirred to culture solution in retort.
The beneficial effects of the utility model are：
It avoids bubble from entering cell separator using gas-liquid separator, effectively prevents bubble and enter being total to for cell separator
To influence rejection effect in tymapanum；The automation control for realizing whole system by automatic controller simultaneously improves production effect
Rate；Further more, by the settlement action of gas-liquid separator, part cell settlement can be detached, another part cell with outer circulation into
Enter cell separator, by ultrasonic Separation, the two, which combines, is greatly improved retention efficiency, improves cell density, improves production efficiency.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
Reference numeral：1, retort；2, cell separator；3, ultrasonic power controller；4, peristaltic pump；5, it automatically controls
Device；6, gas-liquid separator；601, inlet；602, liquid outlet；603, outer circulation mouth；7, culture medium；8, flow container is received.
Specific implementation mode
In the following, in conjunction with attached drawing and specific implementation mode, the utility model is described further：
As shown in Figure 1, a kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture, including retort
1, cell separator 2, ultrasonic power controller 3, peristaltic pump 4, automatic controller 5 and gas-liquid separator 6；
The ventilation in the retort 1 by pipeline, the retort 1 is by being equipped with the liquid suction pipe and culture medium 7 of peristaltic pump 4
Be connected, by culture medium 7 culture solution and cell lead in retort 1.
The gas-liquid separator 6 is mounted in retort 1, and the middle part of the gas-liquid separator 6 is provided with inlet 601,
Culture solution, which is entered by inlet 601 in gas-liquid separator 6, carries out gas-liquid separation, and the inlet 601 of gas-liquid separator 6 is maintained at
Under the liquid level of retort 1；The bottom of gas-liquid separator 6 is provided with liquid outlet 602, and liquid outlet 602 is connected with retort 1,
The inlet 601 and liquid outlet 602 are disposed as upward incline structure.Since gas-liquid separator 6 has settlement action, cell
Since gravity is settled after gas-liquid separator 6, part cell settlement can be detached.
The lower section of gas-liquid separator 6 is provided with outer circulation mouth 603, and outer circulation mouth 603 passes through liquid suction pipe and cell separator 2
It is connected, cell separator 2 is connected by being equipped with the liquid suction pipe of peristaltic pump 4 with flow container 8 is received, after receiving the collection retention of flow container 8
Cell, the cell in gas-liquid separator 6 in culture solution is drawn into cell separator 2 under the action of peristaltic pump 4 and passes through ultrasound
Separation carries out retention processing, and two kinds of sedimentation retention methods can effectively improve retention efficiency, improve cell density, improve production effect
Rate；And gas-liquid separator 6 removes the gas in culture solution, due to the effect of gas-liquid separator 6, into cell separator 2
Bubble is not present in culture solution, bubble can be effectively prevented and entered in the resonator of cell separator 2, to improve retention effect
Fruit.
The cell separator 2 can improve cell retention with 3 signal communication of ultrasonic power controller using ultrasonic Separation
Effect；Whole peristaltic pumps 4 among the above with 5 signal communication of automatic controller, automatic controller 5 control peristaltic pump 4 switch
State, automatic controller 5 can individually control the working condition of some peristaltic pump 4, can also control the work of multiple peristaltic pumps 4 simultaneously
Situation improves the degree of automation；The cell separator 2 offers circulation port, and the circulation port is by being equipped with peristaltic pump 4
Liquid suction pipe is connected with the top of gas-liquid separator 6, can remaining culture solution in cell separator 2 be poured into gas-liquid separation again
It is settled again in device 6, improves sedimentation quality.
Switch folder is installed at the circulation port of the cell separator 2, the switch folder is connected with automatic controller 5,
The on off state of the control switch folder of automatic controller 5, when needing again to be extracted the culture solution in cell separator 2,
The opening of switch folder is controlled by automatic controller 5, and controls peristaltic pump 4 and the culture solution in cell separator 2 is extracted into gas-liquid
Sedimentation is re-started in separator 6.
The cell separator 2 is further opened with air inlet, is passed through cooling gas toward air inlet, is reduced for cell separator 2
Operating temperature prevents cell separator 2 from overheating, influences the normal work of cell separator 2.
Blender is installed in the retort 1, the blender is connected with the motor mounted on 1 top of retort,
Motor drives blender to be stirred to culture solution in retort 1, avoids cell precipitation in the tank bottom of retort 1, influences to sink
The amount of degrading.
It will be apparent to those skilled in the art that technical solution that can be as described above and design, make various other
Corresponding change and deformation, and all these changes and deformation should all belong to the protection of the utility model claims
Within the scope of.
Claims (5)
1. a kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture, which is characterized in that including retort,
Cell separator, ultrasonic power controller, peristaltic pump, automatic controller and gas-liquid separator；The retort is by being equipped with
The liquid suction pipe of peristaltic pump is connected with culture medium；The gas-liquid separator is mounted in retort, in the gas-liquid separator
Portion is provided with inlet, and the inlet of gas-liquid separator is maintained under the liquid level of retort；The bottom of gas-liquid separator is arranged
There are liquid outlet, liquid outlet to be connected with retort；Outer circulation mouth is provided with below gas-liquid separator, outer circulation mouth passes through drawing liquid
Pipe is connected with cell separator, and cell separator is connected by being equipped with the liquid suction pipe of peristaltic pump with flow container is received, gas-liquid point
It is drawn into cell separator under the action of peristaltic pump from the cell in culture solution in device and carries out retention processing；The cell point
Be connected to ultrasonic power controller signals from device, the peristaltic pump with automatic controller signal communication.
2. ultrasound-sedimentation combination perfusion system according to claim 1 for zooblast perfusion culture, feature exists
Circulation port is offered in, the cell separator, and the circulation port is by being equipped with the liquid suction pipe and gas-liquid separator of peristaltic pump
Top be connected.
3. ultrasound-sedimentation combination perfusion system according to claim 2 for zooblast perfusion culture, feature exists
In being equipped with switch folder at the circulation port of the cell separator, the switch is pressed from both sides to be connected with automatic controller.
4. ultrasound-sedimentation combination perfusion system according to claim 2 or 3 for zooblast perfusion culture, special
Sign is that the cell separator is further opened with air inlet, and cooling gas is passed through toward air inlet, and work is reduced for cell separator
Temperature.
5. ultrasound-sedimentation combination perfusion system according to claim 1 for zooblast perfusion culture, feature exists
In being equipped with blender in the retort, the blender is connected with the motor at the top of retort, and motor drives
Blender is stirred culture solution in retort.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820138285.9U CN207944110U (en) | 2018-01-27 | 2018-01-27 | A kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820138285.9U CN207944110U (en) | 2018-01-27 | 2018-01-27 | A kind of ultrasound-sedimentation combination perfusion system for zooblast perfusion culture |
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| CN207944110U true CN207944110U (en) | 2018-10-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114599775A (en) * | 2019-11-07 | 2022-06-07 | 默克专利股份公司 | Methods and systems for performing perfusion cell culture |
-
2018
- 2018-01-27 CN CN201820138285.9U patent/CN207944110U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114599775A (en) * | 2019-11-07 | 2022-06-07 | 默克专利股份公司 | Methods and systems for performing perfusion cell culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: An ultrasonic sedimentation combined perfusion system for animal cell perfusion culture Effective date of registration: 20210901 Granted publication date: 20181009 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: GUANGZHOU RECOMGEN BIOTECH Co.,Ltd. Registration number: Y2021980008729 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| CP03 | Change of name, title or address |
Address after: 510000 No.1, jinfengyuan Road, Guangzhou Economic and Technological Development Zone, Guangdong Province Patentee after: Shijiazhuang Pharmaceutical Group mingfule Pharmaceutical (Guangzhou) Co.,Ltd. Address before: 510530 No. 1, jinfengyuan Road, Guangzhou Economic and Technological Development Zone, Guangdong Province Patentee before: GUANGZHOU RECOMGEN BIOTECH CO.,LTD. |
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| CP03 | Change of name, title or address | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220926 Granted publication date: 20181009 Pledgee: Guangzhou Caold financing Company limited by guarantee Pledgor: GUANGZHOU RECOMGEN BIOTECH CO.,LTD. Registration number: Y2021980008729 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right |