CN210085434U

CN210085434U - Airlift bioreactor

Info

Publication number: CN210085434U
Application number: CN201822130624.1U
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-12-19
Filing date: 2018-12-19
Publication date: 2020-02-18
Anticipated expiration: 2028-12-19

Abstract

The utility model provides an airlift bioreactor, which belongs to the field of microbial reaction equipment and comprises a tank body, a reactor body and a reactor body, wherein the tank body is internally used for biological reaction; at least two towards the wall pipe, set up and be in the mouth of a jar below of body, towards the wall pipe and be the loop configuration, have the orientation on its pipe wall a plurality of jet orifices of internal wall of jar, towards the wall pipe and include: the first wall punching pipe is an air pipe and is used for being communicated with sterile air; the second wall punching pipe is a liquid pipe and is used for being communicated with sterile water or reaction liquid; the utility model discloses an airlift bioreactor, when carrying out the jar wall and wasing, first dashing the wall pipe and keeping higher frequency with the mode of pulse and clear the wall, the second dashes the wall pipe and keeps lower frequency with the mode of pulse and clear the wall to make the reaction mass who glues hanging on the internal wall of jar can always by effectual clean up, guaranteed reaction mass's survival rate, improved the purity of reaction product.

Description

Airlift bioreactor

Technical Field

The utility model relates to a microbial reaction equipment field, concretely relates to airlift bioreactor.

Background

The bioreactor is a device for providing a sterile, proper-temperature, well-nourished and sufficient oxygen supply growth environment for bacteria, fungi, plant cells, animal cells and the like, wherein the airlift bioreactor is one of reactors in the field of plant cell culture and is applied to the plant cell culture or fermentation industry.

The airlift bioreactor is a new reactor developed on the basis of the traditional bubble tower, and the principle of the airlift bioreactor is to utilize the jet kinetic energy of reaction gas and the circulating flow of liquid to stir reaction materials, and the airlift bioreactor is formed by adding a guide cylinder into the traditional bubble tower. When gas enters the central guide cylinder through the gas distributor, the density of fluid in the pipe is lower than that of fluid outside the pipe, and under the action of static pressure difference and momentum of the entering gas, liquid carries bubbles to form circulating flow in the reactor, so that good gas-liquid mixing is achieved. Therefore, compared with other bioreactors, the bioreactor has stronger capacity of resisting the pollution of the foreign bacteria, more uniform fluidity, simple structure of the reactor, no leakage point and sanitary dead angle of reaction liquid and low operation cost.

In order to prevent the reaction liquid from turning over, the conventional airlift bioreactor usually has a wall cleaner for spraying sterile air or sterile water toward the inner wall of the tank in the form of high-voltage pulses to flush the reaction liquid and the reaction material stuck on the inner wall of the tank into the tank and prevent the reaction material from wall-forming necrosis, in order to prevent the reaction liquid from turning over and hanging the reaction material on the inner wall of the tank to cause wall-forming.

Because in the reaction, can often glue some reaction material of hanging on the inner wall of airlift bioreactor, consequently, need carry out the washing of higher frequency to jar internal wall, however, if adopt aseptic water to clear up, in order to keep jar internal thing liquid balanced, can't realize the long-term or short-term many times clear up jar internal wall, and if adopt aseptic air to clear up, again because pressure restriction, can't guarantee to effectively clear up all reaction material of hanging on jar internal wall completely, consequently, no matter what kind of medium cleans up, can't avoid, some reaction material still can bond on the inner wall, cause the death after long-time, will pollute the internal reaction product of jar.

SUMMERY OF THE UTILITY MODEL

Therefore, the to-be-solved technical problem of the utility model lies in overcoming the airlift bioreactor among the prior art, can't carry out the effective clearance of higher frequency to jar internal wall, leads to partial reaction material to become the dead defect of wall easily to provide one kind and can adopt higher frequency to clear the wall, and guarantee to effectively clear away the reaction material who glues the hanging on jar internal wall, avoid reaction material to leave the airlift bioreactor that leads to death after the reaction liquid for a long time.

In order to solve the above technical problem, the utility model provides an airlift bioreactor, include:

the tank body is internally used for biological reaction;

at least two towards the wall pipe, set up and be in the mouth of a jar below of body, towards the wall pipe and be the loop configuration, have the orientation on its pipe wall a plurality of jet orifices of internal wall of jar, towards the wall pipe and include:

the first wall punching pipe is an air pipe and is used for being communicated with sterile air;

and the second wall flushing pipe is a liquid pipe and is used for being communicated with sterile water or reaction liquid.

Preferably, the aperture of the jet hole is 1-2 mm.

Preferably, the central line of the jet hole and the inner wall of the tank body form an included angle of 15-30 degrees downwards horizontally.

Preferably, the longitudinal section of the injection hole is a sector with an outward opening.

As a preferred scheme, the pipe diameter of the wall punching pipe is 10-18 mm.

Preferably, the diameter of the ring formed by the wall punching pipe is smaller than the inner diameter of the tank body by 100-200 cm.

Preferably, the distance between the wall punching pipe and the upper opening of the tank body is 60-120 cm.

Preferably, the outer wall of the tank body is provided with a jacket.

As the preferred scheme, be equipped with the lateral wall window on the outer wall of jar body, the lateral wall window passes through transparent material's board and seals.

Preferably, a top cover window is arranged on a tank cover arranged on the tank body upper cover and is sealed by a turnover gland.

Preferably, the interval between the plurality of punched wall pipes is 10 cm.

Preferably, the injection holes are distributed uniformly over the annular structure formed by the die wall tube.

Preferably, the distance between the central lines of the plurality of jet holes is 6-15 cm.

The technical scheme of the utility model, have following advantage:

1. the utility model provides an airlift bioreactor, two at least towards the wall pipe of arranging about the upper port department of the jar body has, wherein first towards the wall pipe is used for the aseptic air of intercommunication, the second towards the wall pipe and is used for intercommunication aseptic water or reaction liquid, when carrying out jar wall washing, first towards the wall pipe keeps the higher frequency with the pulsed mode and clears up the wall, the second towards the wall pipe keeps the lower frequency with the pulsed mode and clears up the wall, thereby make the reaction mass who glues hanging on the internal wall of jar can always by effectual clean up, the survival rate of reaction mass has been guaranteed, the purity of reaction product has been improved.

2. The utility model provides an air-lift bioreactor, jet orifice have less aperture, make from jet orifice spun air or water have higher pressure to can improve the clearance effect to jar internal wall.

3. The utility model provides an airlift bioreactor, the longitudinal section of jet orifice is opening sector outwards, can enlarge and wash the area, increases and washes the coverage rate of medium to jar internal wall, improves the validity of clearing up.

4. The utility model provides an airlift bioreactor, the central line of jet orifice set up to become the downward contained angle of 15 ~ 30 of level with the inner wall of the jar body, make from the downthehole spun flushing medium of jet orifice, can be faster contact the wall built-up reaction material to effectual reaction material is cleared up.

5. The utility model provides an airlift bioreactor, towards the wall pipe in the wall-cleaning operation process, each other noninterference, through applying soft impact force to the reaction material of wall built-up, make reaction material be washed back to jar body in, continue to react.

6. The utility model provides an airlift bioreactor, the cover that presss from both sides that establishes on the outer wall of the jar body for keep the internal reaction temperature of jar, improve the reaction of reactor.

7. The utility model provides an airlift bioreactor, the lateral wall window that sets up on the outer wall of the jar body seals through transparent material such as glass board for can observe the wall built-up phenomenon of jar internal portion at any time, thereby in time clear up the reaction material who joins the wall through manual.

8. The utility model provides an airlift bioreactor sets up the top cap window on the cover, can open and close through the upset gland for more convenient interpolation of carrying out reaction material opens repeatedly of cover when avoiding adding reaction material, has guaranteed the stability of jar internal reaction condition.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a front view of the present invention.

Fig. 2 is a front view of the installation position of the wall punching pipe in the tank body.

Description of reference numerals:

1. a tank body; 2. a can lid; 3. a jacket; 4. discharging the air pipe; 5. discharging the material pipe; 6. a first wall punching pipe; 7. a second wall punching pipe; 8. a top cover window; 9. a gland; 10. a sidewall window.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.

Example 1

The airlift bioreactor provided in this embodiment, as shown in fig. 1 and fig. 2, includes: a tank body 1, a tank cover 2 and a wall punching pipe.

The biological reaction is used for in the jar body 1, is equipped with on the outer wall of jar body 1 and presss from both sides cover 3, is equipped with lateral wall window 10 on the outer wall of jar body 1, lateral wall window 10 seals through transparent glass board. The tank body 1 is also provided with an air release pipe 4 and a material release pipe 5, the air release pipe 4 is used for maintaining the air pressure in the tank body 1, and the material release pipe 5 is used for discharging reaction products.

The tank cover 2 is covered at the upper opening of the tank body 1, the tank cover 2 is provided with a top cover window 8, and the top cover window 8 is sealed by a turnover gland 9.

The wall-punching tube has two: the first wall pipe 6 and the second wall pipe 7 of dashing, wherein the first wall pipe 6 of dashing is the air pipe for with aseptic air intercommunication, the second wall pipe 7 of dashing is the liquid pipe, is used for with aseptic water or reaction liquid intercommunication. The two wall punching pipes are of annular structures and are arranged below the upper opening of the tank body 1, a plurality of injection holes facing the inner wall of the tank body 1 are formed in the pipe walls of the wall punching pipes, the injection holes are uniformly distributed on the annular structure formed by the wall punching pipes, the distance between the center lines of the injection holes is 6-15 cm, the aperture of each injection hole is 1-2 mm, and the center line of each injection hole and the inner wall of the tank body 1 form an included angle of 15-30 degrees downwards horizontally; the pipe diameter of the wall punching pipe is 10-18 mm, the diameter of the ring formed by the wall punching pipe is smaller than the inner diameter of the tank body 1 by 100-200 cm, the distance between the wall punching pipe and the upper opening of the tank body 1 is 60-120 cm, and the interval between the wall punching pipes is 10 cm.

Application method

When wall cleaning is carried out, clean air with the pressure of 0.2MPa is introduced into the first wall flushing pipe 6, the inner wall of the tank body is cleaned by injecting air through the electromagnetic valve with high-frequency pulses, clean water with the pressure of 0.2MPa is introduced into the second wall flushing pipe 7, the inner wall of the tank body is cleaned by spraying water through the electromagnetic valve with low-frequency pulses, wherein the frequency of injecting air and spraying water can be adjusted according to the actual cleaning condition.

In addition, the electromagnetic valve for controlling the second wall punching pipe 7 to spray water can be manually controlled according to the field situation, so that the wall can be cleaned by manually controlling the water spraying when necessary.

Meanwhile, reaction liquid can be introduced into the second wall flushing pipe 7 and used for supplementing the reaction liquid in the tank body.

In an alternative embodiment, the longitudinal section of the injection hole is a sector with an outward opening.

As an alternative, a plurality of said injection holes may be distributed non-uniformly over the annular structure formed by the die wall tube.

As an alternative embodiment, the distance between the center lines of the injection holes may be set to be out of the range of 6-15 cm according to the field situation.

As an alternative embodiment, a communication pipe is provided between the first wall punching pipe 6 and the second wall punching pipe 7 outside the tank 1, and a switching valve is provided on the communication pipe, by which a medium passing through the first wall punching pipe 6 and the second wall punching pipe 7 can be changed.

As an alternative embodiment, the punched wall tubes may be provided in three or more groups.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.

Claims

1. An airlift bioreactor, comprising:

a tank body (1) for carrying out a biological reaction therein;

at least two towards the wall pipe, set up the mouth of a river below of the jar body (1), it is the loop configuration to dash the wall pipe, has the orientation on its pipe wall a plurality of jet orifices of the internal wall of the jar body (1), it includes to dash the wall pipe:

the first wall punching pipe (6) is an air pipe and is used for being communicated with sterile air;

and the second wall punching pipe (7) is a liquid pipe and is used for being communicated with sterile water or reaction liquid.

2. An airlift bioreactor as set forth in claim 1 wherein said jet orifice has a diameter of 1 to 2 mm.

3. An airlift bioreactor as set forth in claim 2 wherein the centerline of said jet orifice is at an angle of 15-30 ° downward from the horizontal to the inner wall of said tank (1).

4. An airlift bioreactor as set forth in claim 1 wherein said jet orifices have a longitudinal cross-section in the form of a sector having an outwardly opening face.

5. An airlift bioreactor as set forth in claim 1 wherein said impact wall tube has a tube diameter of 10-18 mm.

6. An airlift bioreactor as claimed in claim 5 wherein the diameter of the annulus formed by the impact wall tube is less than the internal diameter of the tank (1) by 100-200 cm.

7. An airlift bioreactor as claimed in claim 5 wherein said impact wall tube is 60-120 cm from the upper mouth of said tank (1).

8. Airlift bioreactor according to claim 1, wherein the outer wall of the tank (1) is provided with a jacket (3).

9. Airlift bioreactor according to claim 1, characterized in that the outer wall of the tank (1) is provided with a side window (10), the side window (10) being closed by a plate of transparent material.

10. Airlift bioreactor according to claim 1, characterized in that the tank lid (2) provided on the tank body (1) is provided with a lid window (8), said lid window (8) being closed by a reversible gland (9).