CN205933846U - Cell in vitro culture ware - Google Patents
Cell in vitro culture ware Download PDFInfo
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- CN205933846U CN205933846U CN201620927212.9U CN201620927212U CN205933846U CN 205933846 U CN205933846 U CN 205933846U CN 201620927212 U CN201620927212 U CN 201620927212U CN 205933846 U CN205933846 U CN 205933846U
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Abstract
The utility model discloses a cell in vitro culture ware, including base and a plurality of cultivation household utensils, each is trained household utensils and stacks gradually, trains household utensils and includes dish body, vertical cylinder and annotate the liquid pipe, and the equal opening in the upper and lower both ends of vertical cylinder, a swinging head pass all vertical cylinder, and the swinging head can rotate with vertical cylinder relatively, and sealing contact between swinging head and the vertical cylinder, is equipped with the displacement tank on the swinging head lateral wall, in cultivation household utensils: vertical cylinder is last be equipped with with the equal liquid hole of the inside intercommunication of dish body, downthehole cell filtration net and the equal liquid check valve of being equipped with of equal liquid, equal liquid check valve is in between cell filtration net and the swinging head, the accessible orientation of equal liquid check valve be by in the dish body outside dish body, annotate liquid pipe lower extreme and dish body is inside feeds through. The beneficial effects of the utility model are that: be applicable to cell culture and survey, compare, the reasonable and stable in structure of spatial layout, operation process is comparatively convenient, and regulation uniformity in the process of the test is good, the accuracy is high.
Description
Technical field
This utility model belongs to technical field of cell culture, more particularly, to a kind of cell injuring model device.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, and the nutrient matrix for maintaining cell growth is referred to as culture medium.Culture medium can be divided into by its physical state
Fluid medium and solid medium.Cell culture technology, in addition to being applied to produce, is more for testing at present（As right
Group sample parameter is measured, and compares and study）, and test in the more commonly used to be fluid medium.Additionally, it is thin
Born of the same parents' culture needs to use cell culture system, and the cell culture that common are under culture dish, incubator etc., general environment can be straight
Connect and use culture dish, if desired for some special environments（As energising, heating etc.）, then special incubator can be used.Exist at present
Carry out cell culture and during Comparability test when although common culture apparatuses can play for culture medium provide carry
The function in space, but at aspects such as space layout reasonability, integrated operation convenience, regulating effect concordance, be still short of.
Utility model content
This utility model is to overcome deficiency of the prior art, there is provided one kind be applied to cell culture and mensure,
Compare, rationally and Stability Analysis of Structures, operating process is more convenient, and the regulation concordance in process of the test is good, accuracy for space layout
High incubator.
To achieve these goals, this utility model employs the following technical solutions：
A kind of cell injuring model device, including the culture vessel of base and some upper end open, each culture vessel by down to
On stack gradually, culture vessel contact base of bottom, described base is provided with fixing vertical rod, culture vessel lateral wall
It is provided with the retaining collar being adapted to fixing vertical rod, retaining collar is set in fixing vertical rod, and culture vessel includes ware body, setting
Column on bottom in ware body and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, the equal opening of column upper/lower terminal,
Through all columns, described rotation pipe and column can relatively rotate the rotation pipe of one vertical arrangement, and rotate pipe lateral wall and
In sealing contact between column medial wall, rotate pipe lateral wall and be provided with and run through the sump pit rotating pipe upper/lower terminal；
In a culture vessel：Column is provided with the equal liquid hole connecting with portion in ware body, is provided with cell mistake in equal liquid hole
Filter screen, liquid injection pipe lower end is connected with portion in ware body；
Described liquid injection pipe upper end is provided with the lid with the cooperation of fluid injection pipe screw thread, rotates and is arranged with for by the top on pipe
The vessel cover that the upper end open of one culture vessel seals；
Described column includes cylinder and orifice plate, and cylinder lateral wall is provided with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made up of mutually symmetrical with upper half plate and lower half plate, upper half plate and lower half
Plate is formed in one structure, and equal liquid hole is located on upper half plate.
Preferably, described base includes matrix and some support columns being arranged on below matrix, matrix is provided with and runs through
The bottom through hole on the upper and lower surface of matrix, rotates pipe lower end and stretches in the through hole of bottom, be provided with below the through hole of bottom opening up connect fluid cylinder,
It is provided with the bottom bracing plate being connected with least one support column, bottom bracing plate is provided with and connects the cylinder groove that fluid cylinder bottom is adapted to below matrix,
Connect fluid cylinder bottom to be in a groove.
Preferably, being provided with core pipe and some horizontally disposed diaphragm plates in described rotation pipe, core pipe lower end is stretched into bottom and is led to
In the hole, core pipe is arranged with support ring, and support ring is higher than core pipe bottom and support ring is in the through hole of bottom, is provided with and props up in the through hole of bottom
Support seat, support base top is provided with seat slot and the annular groove being adapted to rotation pipe lower end, and support ring bottom contacts seat slot bottom land, rotates pipe
Lower end is stretched in annular groove and is rotated bottom of the tube contact annular groove bottom land, rotates pipe and is rotatably assorted with support base, core pipe passes through support base
And core pipe is rotatably assorted with support base, diaphragm plate is set in core pipe, diaphragm plate cross section be in annular, rotate pipe, core pipe and
Each diaphragm plate is all coaxial, and core pipe can relatively rotate with diaphragm plate, in sealing contact between core pipe and diaphragm plate, and diaphragm plate is managed with rotating
Between sealing fix；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and tabula
Plate corresponds, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts diaphragm plate
Top surface；
Rotate in pipe and between adjacent two pieces of diaphragm plates, form quantitative chamber, if rotating pipe to be provided with main to fluid apertures, quantitative chamber
Correspond to fluid apertures with pipe, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：On column
It is provided with cylinder to fluid apertures, quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe is all in justify with cylinder to fluid apertures to fluid apertures
Cylindricality, and the pipe interconnecting is coaxial to fluid apertures with cylinder to fluid apertures, cylinder is given higher than cylinder to fluid apertures axis horizontal, quantitative chamber lowest part
Fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
Preferably, in corresponding quantitative chamber with culture vessel：Quantitative intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule sets
Have for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end is connected with tune amount liquid capsule, feed pipe is by being in quantitative intracavity
Hard tube section and the outer segment being in outside quantitative chamber are constituted；
Base be provided with some for the confession fluid cylinder to feed pipe feed flow, include cylinder body and cylinder body slipper seal for fluid cylinder
Cooperation feed flow piston and be connected and be in the piston rod above feed flow piston, the slidably direction of feed flow piston with feed flow piston
For above-below direction, supply in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity, above feed flow piston in cylinder body
Space is to adjust chamber；
Sap cavity is corresponded with feed pipe, and the feed pipe other end is connected with corresponding sap cavity, and feed pipe passes through and rotates tube side
Wall and rotation pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
Preferably, described base is provided with the adjustment seat fixing with base, below adjustment seat, it is provided with horizontally disposed tune
Section plate, piston rod passes through adjustable plate, and piston rod is provided with upper holder block and lower drawing block, and adjustment seat is provided with vertically to arrange and pass through and adjusts
The homophony pole of section seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block bottom
Face contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, and main regulation masthead portion is provided with the master fixing with homophony pole
Adjusting knob.
Preferably, described homophony pole is provided with the instruction disk being in above adjustment seat, instruction disk is same with homophony pole
Axle, adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction Pan Cebi.
Preferably, described rotate the sealing depression bar being provided with vertical arrangement in pipe, sealing depression bar pass through all diaphragm plates and
All threaded between sealing depression bar and all diaphragm plates, sealing depression bar is provided with some pressure strips for compressing hole sealing plate,
Pressure strip is corresponded with hole sealing plate, and pressure strip is in the top of corresponding hole sealing plate.
The beneficial effects of the utility model are：It is applied to cell culture and mensure, comparison, space layout is reasonable and structure is steady
Fixed, operating process is more convenient, and the regulation concordance in process of the test is good, accuracy is high.
Brief description
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of this utility model orifice plate；
Fig. 3 is the enlarged drawing at A in Fig. 1；
Fig. 4 is the enlarged drawing at B in Fig. 1；
Fig. 5 is the enlarged drawing at C in Fig. 1；
Fig. 6 is the sectional view that this utility model rotates at pipe.
In figure：Base 1, fixing vertical rod 11, connect fluid cylinder 12, bottom bracing plate 13, support base 14, matrix 1a, support column 1b, bottom lead to
Hole 1c, culture vessel 2, retaining collar 21, equal liquid hole 22, lid 23, vessel cover 24, orifice plate 25, cylinder to fluid apertures 26, ware body 2a,
Column 2b, liquid injection pipe 2c, rotation pipe 3, sump pit 31, diaphragm plate 32, company's lumen pore 321, pipe are to fluid apertures 33, tune amount liquid capsule 34, confession
Liquid funnel 35, quantitative chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, for fluid cylinder 5, cylinder body 5a, feed flow piston 5b, piston rod 5c,
Sap cavity 5d, regulation chamber 5e, adjustment seat 6, adjustable plate 61, upper holder block 611, lower drawing block 612, homophony pole 62, main regulation knob
621st, instruction disk 622, indicating arm 63, sealing depression bar 7, pressure strip 71.
Specific embodiment
With reference to the accompanying drawings and detailed description this utility model is further described.
In embodiment as shown in Figures 1 to 6, a kind of cell injuring model device, including base 1 and some upper end open
Culture vessel 2, each culture vessel stacks gradually from the bottom to top, and a culture vessel contact base of bottom, on described base
It is provided with fixing vertical rod 11, culture vessel lateral wall is provided with the retaining collar 21 being adapted to fixing vertical rod, and retaining collar is set in
In fixing vertical rod, culture vessel include ware body 2a, the column 2b being arranged on bottom in ware body and be arranged on ware body sidewall and
The liquid injection pipe 2c being in tilted layout, the equal opening of column upper/lower terminal, a rotation pipe 3 vertically arranged passes through all columns, described
Rotate pipe can relatively rotate with column, and rotate in sealing contact between pipe lateral wall and column medial wall, rotate on pipe lateral wall
It is provided with and runs through the sump pit 31 rotating pipe upper/lower terminal, in a culture vessel：Column is provided with and is connected with portion in ware body
Equal liquid hole 22, is provided with cell filtering net in equal liquid hole, liquid injection pipe lower end is connected with portion in ware body.The cross section of fixing vertical rod is in square
Shape.Retaining collar is made to be inserted in when before test, from the bottom to top each culture vessel can be stacked gradually placement, and place fixing vertical
Bar, so that column is covered outer to rotating pipe（The structure rotating in pipe and rotation pipe can pre-set）, so that each culture vessel
Can align, the culture vessel of top just covers the culture vessel of lower section.Then liquid feeding is carried out to each liquid injection pipe（Add liquid
Body culture medium or culture fluid）, deliberately need not control liquid volume added during liquid feeding, as long as liquid level lid crosses equal liquid hole.All liquid feeding is complete
Cheng Hou, turn rotates pipe, allows sump pit to connect with each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid hole stream
Go out, in culture vessel, remaining liquid measure reaches unanimously, subsequently turn can rotate pipe, allow sump pit leave equal liquid hole（It is made not connect
Logical）, so just it has been rapidly completed liquid feeding.
It is pointed out that treating that cultured cells can just insert culture vessel before adding culture fluid（Need to wait to cultivate
Cell of short duration can leave culture fluid and non-inactivation or death）, now due to there being the presence of cell filtering net, cell will not flow out
Culture vessel；Can also add in culture fluid, equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process）Add afterwards and treat cultured cells；Also the culture fluid that directly band can be needed cultured cells is directly injected into training
Foster vessel（Test under the premise of being somebody's turn to do should not consider the impact of initial cell quantity, and due to having the presence of cell filtering net, cell
Will not flow out culture vessel）.Specifically can be selected according to test objective, demand.
Described liquid injection pipe upper end is provided with the lid 23 with the cooperation of fluid injection pipe screw thread, rotates and is arranged with for by the top on pipe
A culture vessel the vessel cover 24 that seals of upper end open.Lid can block and rotate pipe and extraneous connection, vessel cover
A culture vessel of the top and extraneous connection can be blocked, remaining culture vessel is then by a nearest training above itself
Foster vessel block and extraneous connection.Such that it is able to ensure that each culture vessel separated with the external world in cell culture, process of the test,
Thus the test under the premise of allowing this utility model to be applicable to be hedged off from the outer world.
Described base includes matrix 1a and some support column 1b being arranged on below matrix, and matrix is provided with and runs through matrix
The bottom through hole 1c on upper and lower surface, rotates pipe lower end and stretches in the through hole of bottom, be provided with below the through hole of bottom opening up connect fluid cylinder 12,
It is provided with the bottom bracing plate 13 being connected with least one support column, bottom bracing plate is provided with and connects the cylinder that fluid cylinder bottom is adapted to below matrix
Groove, connects fluid cylinder bottom and is in a groove.The liquid discharged from sump pit can be reclaimed easily（Fall into and connect fluid cylinder）, wave can be avoided
Take and avoid polluting to external world.
Described column includes cylinder and orifice plate 25, and cylinder lateral wall is provided with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made up of mutually symmetrical with upper half plate and lower half plate, upper half plate and lower half
Plate is formed in one structure, and equal liquid hole is located on upper half plate.In addition to equal liquid hole, orifice plate is laterally zygomorphic structure, consequently, it is possible to
One piece of orifice plate can be achieved with the Liquid level of two kinds of differing heights, i.e. by orifice plate with upper half plate upper and lower half plate under in the form of
Snap in side opening, then through equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）
Afterwards, in culture vessel, liquid level is consistent with equal liquid hole lowest part（Now equal liquid hole is in more than in the middle part of orifice plate）；And by orifice plate
With upper half plate under, lower half plate snap in side opening in upper form, then liquid level and equal liquid hole lowest part in final culture vessel
Unanimously（Now equal liquid hole is in below in the middle part of orifice plate）.Furthermore, it is possible to configure many set orifice plates, between each set orifice plate, only has equal liquid hole
Highly different, so can be achieved with the quick control of various different initial liquid measures in culture vessel（Needed which liquid level, just
A set of orifice plate corresponding with this liquid level is snapped in advance and is used in the side opening of each culture vessel）.
It is provided with core pipe 4 and some horizontally disposed diaphragm plates 32, core pipe lower end is stretched in the through hole of bottom, core in described rotation pipe
Support ring 41 is arranged with pipe, support ring is higher than core pipe bottom and support ring is in the through hole of bottom, is provided with support base in the through hole of bottom
14, support base top is provided with seat slot and the annular groove being adapted to rotation pipe lower end, and support ring bottom contacts seat slot bottom land, rotates under pipe
End is stretched in annular groove and is rotated bottom of the tube contact annular groove bottom land, rotates pipe and is rotatably assorted with support base, core pipe through support base and
Core pipe is rotatably assorted with support base, and diaphragm plate is set in core pipe, and diaphragm plate cross section is in annular, rotates pipe, core pipe and each
Diaphragm plate is all coaxial, core pipe can relatively rotate with diaphragm plate, in sealing contact between core pipe and diaphragm plate, diaphragm plate with rotate pipe
Between sealing fix；
Diaphragm plate is provided with even lumen pore 321, and core pipe is provided with some hole sealing plates 42 for company of sealing lumen pore, hole sealing plate and
Diaphragm plate corresponds, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and the contact of hole sealing plate bottom surface is horizontal
Dividing plate top surface；
Rotate in pipe and between adjacent two pieces of diaphragm plates, form quantitative chamber 3a, if rotating pipe to be provided with main to fluid apertures 33, fixed
Amount chamber and pipe correspond to fluid apertures, and quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：Perpendicular
Cylinder is provided with cylinder to fluid apertures 26, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is to fluid apertures and cylinder to fluid apertures
All in cylinder, and the pipe interconnecting is coaxial to fluid apertures with cylinder to fluid apertures, and, to fluid apertures axis horizontal, quantitative chamber lowest part is high for cylinder
In cylinder to fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part；
Rotate pipe top to be provided with and rotate the feed flow funnel 35 that pipe screw thread is connected.During each structure installation, cooperation, if
Feed flow funnel can interfere, then can first unload feed flow funnel, after the completion of to be assembled, operation, put in back feed flow funnel, and continue
Continue and carry out subsequent operation.
When needs carry out mid-term liquid feeding simultaneously to each culture vessel（New culture fluid, Ke Yiyu is added in incubation
Initial incubation liquid species, proportioning identical it is also possible to different from initial incubation liquid species, proportioning）When, for each ware body, liquid
The time difference that body adds is less, and during final mensure, the interference of suffered exopathogenic factor is also less（It is preferably capable of being simultaneously introduced culture
Liquid）.But unless allowed many people to carry out liquid feeding to each culture vessel simultaneously, and height while property to be ensured, can be only achieved more
Preferably effect, and in practical operation, the feasibility of this mode is low.And in this programme, first turn rotates pipe, allow pipe
Stagger between fluid apertures with corresponding cylinder to fluid apertures（Do not connect）, then allow rotation manage motionless, first turn core pipe（If rotate pipe with
Between column, frictional force is sufficiently large, then can direct turn core pipe, now rotating pipe will not be driven；If rotate rubbing between pipe and column
Wiping power is limited, then can pinch rotation pipe on the other hand, and another handss carry out turn core pipe；This utility model is in design, preferentially previous
Plant situation）, drive hole sealing plate to leave even lumen pore（Allow the shrouding not company's of sealing lumen pore）, then can carry out liquid feeding from rotating pipe top,
Culture fluid through each even lumen pore, and finally can be full of all of quantitative chamber, then proceedes to allow rotation manage motionless, turn core pipe,
Make hole sealing plate seal even lumen pore, separate between now each quantitative chamber, and each quantitative intracavity liquid measure is consistent.Then can turn rotate
Pipe, drives core pipe to rotate together by diaphragm plate（It is fixing between diaphragm plate and rotation pipe, although between core pipe and diaphragm plate
Can relatively rotate, but due in sealing contact between core pipe and diaphragm plate, therefore between core pipe and diaphragm plate, necessarily have mutual compression
Power and frictional force, so if will relatively rotating, need the external force applying to allow them to relatively rotate.And only turn core pipe when,
In the presence of frictional force, diaphragm plate can rotate together with core pipe, thus rotate pipe and core pipe between relative position not
Become）, allow pipe to connect between fluid apertures with corresponding cylinder to fluid apertures, now each quantitative chamber can be same to portion in corresponding ware body
When, equivalent liquid feeding, can thoroughly eliminate during middle liquid feeding the interference such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent because
Element.Additionally, this programme except can be used for mid-term liquid feeding it can also be used to initially when liquid feeding.And after having support base, from discharge opeing
The liquid that groove is discharged can first reach support base top, then outwards flows down.
It is noted that when sump pit is connected with equal liquid hole, pipe is staggered between fluid apertures with corresponding cylinder to fluid apertures；Manage to
To between fluid apertures, during connection, sump pit is staggered fluid apertures with equal liquid hole with corresponding cylinder.And pass through equal hydraulic control amount（I.e. mentioned by equal
Fluid apertures carries out discharge opeing so that the consistent process of liquid measure in each ware body）Afterwards, carry out the behaviour of liquid feeding in using quantitative chamber to ware body
During work（When turn rotates pipe）It is impossible to allow sump pit to connect again with equal liquid hole, leakage at sump pit otherwise can be led to
（In ware body, liquid flows out from equal liquid hole, sump pit again）, specific constructive form and mode of operation have many kinds, in this measure two
Example,
Example 1：In the circumference rotating pipe, sump pit, equal liquid hole, pipe are to fluid apertures, cylinder to fluid apertures along up time pointing needle successively
Arrangement.All hydraulic control amounts（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath is clockwise to alignment equal liquid hole with rotating pipe（During being somebody's turn to do, pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）, all
After the completion of hydraulic control amount, sump pit is continued to rotate clockwise and is staggered with equal liquid hole, now pipe to fluid apertures and corresponding cylinder to fluid apertures according to
So being not reaching to connected state, if subsequently continuing turn clockwise to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures；
Example 2：In the circumference rotating pipe, equal liquid hole, sump pit, pipe are to fluid apertures, cylinder to fluid apertures along clockwise direction successively
Arrangement, equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath rotates counterclockwise to alignment equal liquid hole with rotating pipe（During being somebody's turn to do, pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）, all
After the completion of hydraulic control amount, sump pit is rotated clockwise and is staggered with equal liquid hole, and now pipe is not still had to fluid apertures with corresponding cylinder to fluid apertures
Having and reach connected state, if subsequently continuing turn clockwise to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber with culture vessel：Quantitative intracavity is provided with tune amount liquid capsule 34, tune amount liquid capsule be provided with for
To the feed pipe of tune amount liquid capsule feed flow, feed pipe one end is connected with tune amount liquid capsule, and feed pipe is by the hard tube section being in quantitative intracavity
And the outer segment being in outside quantitative chamber is constituted；
Base be provided with some for the confession fluid cylinder 5 to feed pipe feed flow, for fluid cylinder include cylinder body 5a and cylinder body slide close
The feed flow piston 5b of envelope cooperation and be connected and be in the piston rod 5c above feed flow piston with feed flow piston, the sliding of feed flow piston
Dynamic direction is above-below direction, supplies in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity 5d, and in cylinder body, feed flow is lived
The space of side is to adjust chamber 5e beyond the Great Wall；
Sap cavity is corresponded with feed pipe, and the feed pipe other end is connected with corresponding sap cavity, and feed pipe passes through and rotates tube side
Wall and rotation pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
Described base is provided with the adjustment seat 6 fixing with base, is provided with horizontally disposed adjustable plate 61 below adjustment seat, lives
Stopper rod passes through adjustable plate, and piston rod is provided with upper holder block 611 and lower drawing block 612, and adjustment seat is provided with vertical arrangement and through tune
The homophony pole 62 of section seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, and main regulation masthead portion is provided with fixing with homophony pole
Main regulation knob 621.
Described homophony pole is provided with the instruction disk 622 being in above adjustment seat, and instruction disk is coaxial with homophony pole, adjusts
Seat is provided with the indicating arm 63 of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction Pan Cebi.
As it was previously stated, this utility model can carry out equivalent, simultaneously liquid feeding in each ware body.And in this programme, also may be used
To regulate and control to liquid volume added.At the beginning, tune amount liquid capsule is vacuum, if now specified rate chamber is full of, liquid volume added is exactly quantitative chamber
Actual volume.When needing to control liquid volume added, can be with turn homophony pole（Main regulation knob）, make homophony pole upwards or to
Lower movement, taking move down as a example：The upper holder block that now homophony pole passes through on homophony pole drives adjustable plate vertically to move down（On
Briquetting rotates, and adjustable plate does not turn）, adjustable plate pushes down on each feed flow piston by each lower drawing block on piston rod, in each sap cavity
Liquid（Can be water or other liquid, as long as in stable condition, the structures such as the pipeline passing through will not be damaged）Note
Enter corresponding tune amount liquid capsule, now, the actual volume in quantitative chamber（Actual volume refers to accommodate the volume of culture fluid）Become
Little, and the numerical value specifically diminishing, can read from indicating arm（Scale groove on indicating arm can mark real with quantitative chamber
Numeral corresponding to the volume of border, the occurrence of numeral, when manufacturing and designing, can be converted with measured in advance.Can do on instruction disk
Go out labelling, be easy to the reading directly being alignd come mark-sense according to labelling, or, instruction disk itself can also come as labelling
Use, be such as used as labelling using instruction disk top surface or bottom surface）.The actual volume in quantitative chamber can be diminished by said process, from
And control liquid volume added（So that it is reduced）；Conversely, allowing homophony pole to move up, then the liquid in tune amount liquid capsule can be extracted out, from
And can control liquid volume added（So that it is increased）.It is noted that feed pipe by the hard tube section being in quantitative intracavity and is in quantitative chamber
Outer outer segment is constituted, and wherein outer segment can not be perfect rigidity, to guarantee that diaphragm plate, core pipe etc. being capable of normal rotation（Core
Pipe is connected with outer segment, and when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity）, outer segment
The pipe of the type such as sebific duct, oil cylinder petroleum pipeline can be selected.
It is provided with the sealing depression bar 7 of vertical arrangement, sealing depression bar passes through all diaphragm plates and seals depression bar in described rotation pipe
All threaded between all diaphragm plates, sealing depression bar is provided with some pressure strips 71 for compressing hole sealing plate, pressure strip
Correspond with hole sealing plate, pressure strip is in the top of corresponding hole sealing plate.As it was previously stated, filling it up with liquid, Ran Houfeng in rotation pipe
Orifice plate seals after connecting lumen pore, separates between each quantitative chamber.But, in order to allow hole sealing plate will the thorough company's of sealing lumen pore, hole sealing plate
Mutual extruding and one piece of diaphragm plate of lower contact between is necessarily larger.After being used for multiple times, easily lead to because wearing and tearing larger
Sealing is not enough.And in this programme, hole sealing plate seals after connecting lumen pore, depression bar can be sealed with turn, drive pressure strip to move down and press
Tightly corresponding hole sealing plate, improves sealing further, ensures the cut-off effect between each quantitative chamber.And have sealing depression bar, pressure
After tight plate, during fabrication, too tight without pressure between hole sealing plate and diaphragm plate（Sealing can be protected by pushing of pressure strip
Barrier）, such that it is able to effectively reducing abrasion, increasing the service life.It is noted that after having sealing depression bar, pressure strip, turning allowing
Dynamic pipe is motionless and turn core pipe before, pressure strip should be allowed to be not located at compressing the state of corresponding hole sealing plate, " turning motionless " otherwise occurs
Situation.
Claims (6)
1. a kind of cell injuring model device, is characterized in that, including the culture vessel of base and some upper end open, each culture vessel
Stack gradually from the bottom to top, a culture vessel contact base of bottom, described base is provided with fixing vertical rod, culture vessel
Lateral wall is provided with the retaining collar being adapted to fixing vertical rod, and retaining collar is set in fixing vertical rod, and culture vessel includes ware
Body, the column being arranged on bottom in ware body and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, column upper/lower terminal
All openings, through all columns, described rotation pipe and column can relatively rotate a rotation pipe vertically arranged, and it is outer to rotate pipe
In sealing contact between side wall and column medial wall, rotate pipe lateral wall and be provided with and run through the sump pit rotating pipe upper/lower terminal；
In a culture vessel：Column is provided with the equal liquid hole connecting with portion in ware body, is provided with cell filtering net in equal liquid hole,
Liquid injection pipe lower end is connected with portion in ware body；
Described liquid injection pipe upper end is provided with the lid with the cooperation of fluid injection pipe screw thread, rotates and is arranged with for by one of the top on pipe
The vessel cover that the upper end open of culture vessel seals；
Described column includes cylinder and orifice plate, and cylinder lateral wall is provided with side opening, on a column：Orifice plate is stuck in side opening, hole
In sealing contact between plate and side opening hole wall, orifice plate is made up of mutually symmetrical with upper half plate and lower half plate, and upper half plate with lower half plate is
Integrated formed structure, equal liquid hole is located on upper half plate.
2. a kind of cell injuring model device according to claim 1, is characterized in that, described base includes matrix and some sets
Put the support column below matrix, matrix is provided with the bottom through hole running through the upper and lower surface of matrix, rotate pipe lower end and stretch into bottom through hole
Interior, it is provided with the opening up fluid cylinder that connects below the through hole of bottom, below matrix, be provided with the bottom bracing plate being connected with least one support column, bottom
Fagging is provided with and connects the cylinder groove that fluid cylinder bottom is adapted to, and connects fluid cylinder bottom and is in a groove.
3. a kind of cell injuring model device according to claim 1, is characterized in that, if described rotation pipe in be provided with core pipe and
Dry horizontally disposed diaphragm plate, core pipe lower end is stretched in the through hole of bottom, and core pipe is arranged with support ring, and support ring is higher than core pipe bottom
And support ring is in the through hole of bottom, in the through hole of bottom, it is provided with support base, support base top is provided with seat slot and is adapted to rotating pipe lower end
Annular groove, support ring bottom contacts seat slot bottom land, rotates pipe lower end and stretch in annular groove and rotate bottom of the tube contact annular groove bottom land, turn
Dynamic pipe is rotatably assorted with support base, and core pipe passes through support base and core pipe is rotatably assorted with support base, and diaphragm plate is set in core pipe,
Diaphragm plate cross section is in annular, and rotation pipe, core pipe and each diaphragm plate are all coaxial, and core pipe can relatively rotate with diaphragm plate, core pipe
In sealing contact and diaphragm plate between, sealing between diaphragm plate and rotation pipe is fixed；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts tabula top of the plate
Face；
Rotate in pipe and form quantitative chamber between adjacent two pieces of diaphragm plates, if rotating pipe to be provided with main to fluid apertures, quantitative chamber and pipe
Correspond to fluid apertures, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：Column is provided with
Fluid apertures given by cylinder, and quantitative chamber is passed through corresponding pipe and is communicated to cylinder to fluid apertures to fluid apertures, pipe to fluid apertures with cylinder to fluid apertures all in cylinder,
And the pipe that interconnects is coaxial to fluid apertures with cylinder to fluid apertures, to fluid apertures axis horizontal, quantitative chamber lowest part is higher than cylinder to fluid apertures to cylinder
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
4. a kind of cell injuring model device according to claim 3, is characterized in that, in corresponding quantitative chamber and culture vessel
In：Quantitative intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule is provided with for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end with
Tune amount liquid capsule connects, and feed pipe is made up of the hard tube section being in quantitative intracavity and the outer segment being in outside quantitative chamber；
Base be provided with some for the confession fluid cylinder to feed pipe feed flow, include cylinder body and cylinder body slipper seal cooperation for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, supplies in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity, the space above feed flow piston in cylinder body
For adjusting chamber；
Sap cavity and feed pipe correspond, and the feed pipe other end connect with corresponding sap cavity, feed pipe through rotation tube side wall and
Rotate pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
5. a kind of cell injuring model device according to claim 4, is characterized in that, described base is provided with to be fixed with base
Adjustment seat, be provided with horizontally disposed adjustable plate below adjustment seat, piston rod pass through adjustable plate, piston rod be provided with upper holder block and
Lower drawing block, adjustment seat is provided with the vertical homophony pole arranged and pass through adjustment seat, and homophony pole is threadeded with adjustment seat, main
Adjusting rod is provided with upper holder block and lower drawing block, and the contact of upper holder block bottom surface adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface,
Main regulation masthead portion is provided with the main regulation knob fixing with homophony pole.
6. a kind of cell injuring model device according to claim 5, is characterized in that, described homophony pole is provided with and is in tune
Instruction disk above section seat, instruction disk is coaxial with homophony pole, and adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertical
Arrangement and indicating arm contact instruction Pan Cebi.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620927212.9U CN205933846U (en) | 2016-08-22 | 2016-08-22 | Cell in vitro culture ware |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620927212.9U CN205933846U (en) | 2016-08-22 | 2016-08-22 | Cell in vitro culture ware |
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| CN205933846U true CN205933846U (en) | 2017-02-08 |
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| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843A (en) * | 2016-08-22 | 2016-12-07 | 王铁苗 | A kind of multisample culture apparatus |
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| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843A (en) * | 2016-08-22 | 2016-12-07 | 王铁苗 | A kind of multisample culture apparatus |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
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| CN106244457B (en) * | 2016-08-22 | 2018-08-03 | 日照安维信生物科技有限公司 | A kind of experiment culture carrier structure |
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