CN106190843A - A kind of multisample culture apparatus - Google Patents
A kind of multisample culture apparatus Download PDFInfo
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- CN106190843A CN106190843A CN201610701445.1A CN201610701445A CN106190843A CN 106190843 A CN106190843 A CN 106190843A CN 201610701445 A CN201610701445 A CN 201610701445A CN 106190843 A CN106190843 A CN 106190843A
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- pipe
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- culture vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Abstract
The invention discloses a kind of multisample culture apparatus, including base and some culture vessels, each culture vessel stacks, and culture vessel includes that ware body, column, projection and liquid injection pipe, ware body top end face are provided with ware inverted draw cut, base top is provided with base inverted draw cut, the projection of one culture vessel of bottom is in base inverted draw cut, and one rotates pipe and can relatively rotate with column through all columns, rotation pipe, rotate and be in sealing contact between pipe lateral wall and column medial wall, rotate pipe lateral wall and be provided with sump pit；Column is provided with the equal liquid hole connected with portion in ware body, is provided with cell filtration net and equal liquid check valve in equal liquid hole, and liquid injection pipe lower end connects with portion in ware body；In the ware inverted draw cut of the projection culture vessel at the bottom of top culture vessel.The invention has the beneficial effects as follows: be applicable to cell cultivate and measure, comparison, rationally and Stability Analysis of Structures, operating process is the most convenient, and the regulation concordance in process of the test is good, accuracy is high for space layout.
Description
Technical field
The invention belongs to technical field of cell culture, particularly relate to a kind of multisample culture apparatus.
Background technology
Cell is cultivated and is also cell clone technology, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, is referred to as culture medium for the nutrient matrix maintaining cell to grow.Culture medium can be divided into by its physical state
Fluid medium and solid medium.Cell culture technology, in addition to being applied to produce, is the most more for testing (as right
Group sample parameter be measured, and compare and study), and test in the more commonly used to be fluid medium.Additionally, it is thin
Born of the same parents cultivate to be needed to use cell culture system, and common are the cell cultivation under culture dish, incubator etc., general environment can be straight
Connect use culture dish, if desired for some special environments (such as energising, heating etc.), then can use special incubator.Exist at present
Carry out cell cultivation and during Comparability test time, although common culture apparatus can play into culture medium provide carrying
The function in space, but at aspects such as space layout reasonability, integrated operation convenience, regulating effect concordance, be short of.
Summary of the invention
The present invention is to overcome deficiency of the prior art, it is provided that one is applicable to cell and cultivates and mensuration, comparison,
Rationally and Stability Analysis of Structures, operating process is the most convenient, the training that regulation concordance is good, accuracy is high in process of the test for space layout
Support device.
To achieve these goals, the present invention is by the following technical solutions:
A kind of multisample culture apparatus, including base and the culture vessel of some upper end open, each culture vessel depends on from the bottom to top
Secondary stacking, a culture vessel contact base of bottom, described culture vessel includes ware body, is arranged on the interior bottom of ware body
Column, the projection being arranged on the external bottom of ware and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, projection cross section
Rectangular, that ware body top end face is provided with and projection is adaptive ware inverted draw cut, the base inverted draw cut that base top is provided with and projection is adaptive, under
The projection of one culture vessel of side is in base inverted draw cut, and the equal opening of column upper/lower terminal, the rotation pipe of a vertical layout is worn
Crossing all columns, described rotation pipe can relatively rotate with column, and is in sealing contact between rotation pipe lateral wall and column medial wall,
Rotate pipe lateral wall and be provided with the sump pit running through rotation pipe upper/lower terminal；
In a culture vessel: be provided with the equal liquid hole connected with portion in ware body on column, be provided with cell filtration net in equal liquid hole,
Liquid injection pipe lower end connects with portion in ware body, and ware body sidewall is provided with the tune amount transverse tube connected with portion in ware body, and tune amount transverse tube sets
Having the tune amount post running through tune two pipe ends of amount transverse tube, tune amount post is threadeded with tune amount transverse tube, and tune amount post is same with tune amount transverse tube
Axle, tune amount post the inner is in ware body, and tune amount post outer end is in outside tune amount transverse tube, and tune amount post sidewall is provided with baseline trough and is in
Alignment mark rod outside tune amount transverse tube, tune amount transverse tube is provided with baseline rod；
In adjacent two culture vessels: in the ware inverted draw cut of the projection of top culture vessel culture vessel at the bottom；
Described column includes cylinder and orifice plate, and cylinder lateral wall is provided with side opening, on a column: orifice plate is stuck in side opening, hole
Being in sealing contact between plate and side opening hole wall, orifice plate is made up of with lower half plate mutually symmetrical with upper half plate, and upper half plate with lower half plate is
Integrated formed structure, equal liquid hole is positioned on upper half plate；
Described liquid injection pipe upper end is provided with and the lid of liquid injection pipe threaded engagement, rotates and is arranged with for by the one of the top on pipe
The vessel cover that the upper end open of culture vessel seals.
As preferably, described base includes matrix and some support columns being arranged on below matrix, and matrix is provided with and runs through
The end through hole on the upper and lower surface of matrix, rotates pipe lower end and stretches in end through hole, is provided with and opening up connects fluid cylinder below end through hole,
Being provided with the bottom bracing plate being connected with at least one support column below matrix, bottom bracing plate is provided with and connects cylinder groove adaptive bottom fluid cylinder,
Connect and be in bottom fluid cylinder in a groove.
As preferably, being provided with core pipe and some horizontally disposed diaphragm plates in described rotation pipe, it is logical that the end, is stretched in core pipe lower end
In hole, core pipe being arranged with support ring, support ring is higher than bottom core pipe and support ring is in end through hole, is provided with and props up in end through hole
Support seat, supports seat top and is provided with seat slot and the seat apical ring groove adaptive with rotating pipe lower end, contact seat slot bottom land, turn bottom support ring
Contact seat apical ring groove bottom land in an apical ring groove is stretched in dynamic pipe lower end and bottom rotation pipe, rotate pipe and be rotatably assorted with supporting seat, core
Pipe is rotatably assorted through supporting seat and core pipe with support seat, and diaphragm plate is set on core pipe, and diaphragm plate cross section is annular, turns
Dynamic pipe, core pipe and each diaphragm plate are all coaxial, and core pipe can relatively rotate with diaphragm plate, is in sealing contact between core pipe and diaphragm plate, horizontal
Seal fixing between dividing plate and rotation pipe；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, and contact diaphragm plate top, hole sealing plate bottom surface
Face；
Rotate and form quantitative chamber between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole, quantitative chamber and pipe
To fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: be provided with on column
Fluid apertures given by cylinder, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in cylinder,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder to fluid apertures
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
As preferably, in corresponding quantitative chamber with culture vessel: quantitatively intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule sets
Having for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end connects with tune amount liquid capsule, and feed pipe is by being in quantitative intracavity
Hard tube section and the outer segment being in outside quantitative chamber are constituted；
Base is provided with some for the confession fluid cylinder of feed pipe feed flow, including that cylinder body coordinates with cylinder body slipper seal for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, at one in fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity, space above feed flow piston in cylinder body
For regulation chamber；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
As preferably, described base is provided with the adjustment seat fixing with base, is provided with horizontally disposed tune below adjustment seat
Joint plate, piston rod passes adjustable plate, and piston rod is provided with upper holder block and lower drawing block, and adjustment seat is provided with vertically arranges and through adjusting
Saving the homophony pole of seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, at the bottom of upper holder block
Face contact adjustable plate end face, lower drawing block end face contact adjustable plate bottom surface, main regulation masthead portion is provided with the master fixing with homophony pole
Adjusting knob.
As preferably, described homophony pole is provided with the instruction dish being in above adjustment seat, and instruction dish is same with homophony pole
Axle, adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction dish sidewall.
As preferably, in described rotation pipe, be provided with the sealing depression bar of vertically layout, seal depression bar through all diaphragm plates and
Seal depression bar all to threaded between all diaphragm plates, seal depression bar and be provided with some pressure strips for compressing hole sealing plate,
Pressure strip and hole sealing plate one_to_one corresponding, pressure strip is in the top of corresponding hole sealing plate.
The invention has the beneficial effects as follows: be applicable to cell cultivate and measure, comparison, space layout rationally and Stability Analysis of Structures,
Operating process is the most convenient, and the regulation concordance in process of the test is good, accuracy is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of orifice plate of the present invention；
Fig. 3 is the enlarged drawing in Fig. 1 at A；
Fig. 4 is the enlarged drawing in Fig. 1 at B；
Fig. 5 is the enlarged drawing in Fig. 1 at C；
Fig. 6 is the enlarged drawing in Fig. 1 at D；
Fig. 7 is the sectional view that the present invention rotates at pipe.
In figure: base 1, connect fluid cylinder 12, bottom bracing plate 13, support seat 14, matrix 1a, support column 1b, end through hole 1c, incubator
Ware 2, cylinder are to fluid apertures 21, equal liquid hole 22, lid 23, vessel cover 24, orifice plate 25, tune amount transverse tube 26, tune amount post 27, alignment mark rod
28, baseline rod 29, ware body 2a, column 2b, projection 2c, liquid injection pipe 2d, rotation pipe 3, sump pit 31, diaphragm plate 32, even lumen pore
321, pipe is to fluid apertures 33, tune amount liquid capsule 34, feed flow funnel 35, quantitative chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, confession fluid cylinder
5, cylinder body 5a, feed flow piston 5b, piston rod 5c, sap cavity 5d, regulation chamber 5e, adjustment seat 6, adjustable plate 61, upper holder block 611, drop-down
Block 612, homophony pole 62, main regulation knob 621, instruction dish 622, indicating arm 63, sealing depression bar 7, pressure strip 71.
Detailed description of the invention
The present invention will be further described with detailed description of the invention below in conjunction with the accompanying drawings.
In embodiment as shown in Figures 1 to 7, a kind of multisample culture apparatus, including base 1 and some upper end open
Culture vessel 2, each culture vessel stacks gradually from the bottom to top, a culture vessel contact base of bottom, described incubator
Ware includes ware body 2a, the column 2b, the projection 2c being arranged on the external bottom of ware that are arranged on the interior bottom of ware body and is arranged on ware
On body sidewall and the liquid injection pipe 2d that is in tilted layout, projection cross section is rectangular, the ware that ware body top end face is provided with and projection is adaptive
Inverted draw cut, base top is provided with the base inverted draw cut adaptive with projection, and the projection of a culture vessel of bottom is in base inverted draw cut, perpendicular
The cylinder equal opening of upper/lower terminal, the rotation pipe 3 of a vertical layout is through all columns, and described rotation pipe can relative turn with column
Dynamic, and be in sealing contact between rotation pipe lateral wall and column medial wall, rotation pipe lateral wall is provided with runs through rotation pipe upper and lower two
The sump pit 31 of end；
In a culture vessel: be provided with the equal liquid hole 22 connected with portion in ware body on column, be provided with cell filtration in equal liquid hole
Net, liquid injection pipe lower end connects with portion in ware body, and ware body sidewall is provided with the tune amount transverse tube 26 connected with portion in ware body, tune amount transverse tube
Being provided with the tune amount post 27 running through tune two pipe ends of amount transverse tube, tune amount post is threadeded with tune amount transverse tube, and tune amount post and tune amount
Transverse tube is coaxial, and tune amount post the inner is in ware body, and tune amount post outer end is in outside tune amount transverse tube, and tune amount post sidewall is provided with baseline trough
And it being in the alignment mark rod 28 outside tune amount transverse tube, tune amount transverse tube is provided with baseline rod 29；
In adjacent two culture vessels: in the ware inverted draw cut of the projection of top culture vessel culture vessel at the bottom.
Before test, each culture vessel can be stacked gradually placement from the bottom to top, and when placing, make a training of bottom
The projection supporting vessel embeds in base inverted draw cut, make the projection of top culture vessel embed below culture vessel ware inverted draw cut in, make to erect
Jacket casing is to rotating pipe outer (rotation pipe and the structure rotated in pipe can pre-set), so that each culture vessel can be alignd
And fixing, the culture vessel of top just covers the culture vessel of lower section.Then each liquid injection pipe is carried out liquid feeding and (adds liquid
Culture medium or culture fluid), without deliberately controlling liquid volume added during liquid feeding, as long as liquid level lid crosses equal liquid hole.All liquid feeding completes
After, turn rotates pipe, allows sump pit connect with each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid hole stream
Going out, in culture vessel, remaining liquid measure reaches consistent, turn can rotate pipe subsequently, allow sump pit leave equal liquid hole and (make it not connect
Logical), the most just it is rapidly completed liquid feeding.And want to regulate in ware body if liquid measure, it is also possible to realized by turn tune amount post,
And mode is the most various: as, before liquid feeding, tune can be measured post inwardly (outward) turn certain distance, the most whole liquid feeding walks
Suddenly the liquid measure finally stayed in ware body (after the process utilizing equal liquid hole and sump pit to carry out discharge opeing terminates) after completing can reduce and (increase
Add)；And for example, after liquid feeding, sump pit is when connecting with equal liquid hole, tune can be measured post inside turn certain distance, continue to discharge
A part of liquid in ware body, thus allow the liquid measure stayed in ware body reduce.In above-mentioned regulative mode, the latter is more commonly used, this
It is because after once liquid feeding completes, is relatively time-consuming with the mode discharge opeing again drawn, topple over, and operation is the most pretty troublesome, but
Discharge opeing, regulation is carried out by aforementioned manner, the most very convenient.And the fixing quantity mode of tune amount post displacement is the most
Sample, gives one example: in the present embodiment, at the beginning, can allow alignment mark rod align with baseline rod, baseline trough remote with tune amount transverse tube
Align from one end of ware body, can be set as reference position herein and (baseline trough can also be allowed to be aligned in other setting position, depending on making
Depending on the considering when demand of used time, design), after liquid feeding, sump pit is when connecting with equal liquid hole, the often inside turn one of tune amount post
Circle, alignment mark rod will turn one circle again align with baseline rod that (mark rod that aligns, the baseline rod person of being convenient to operation are carried out
Observe and regulation), due to tune amount post inside turn one enclose after, stretch into the volume in ware body be definite value (this value design time just
Have determined that), so the liquid measure discharged also is definite value.During additionally, cultivate, test, tend not to be that all tune fluid columns are all used
Arrive, or each adjust fluid column turn distance can difference, this be in order to adapt to different sample (variety classes cell may needs not
Same initial incubation liquid measure) or quickly provide different culture environment (to come with the identical or different cultivation liquid measure of cell types
Carry out cultivating and subsequent measurements, Comparability test) and the structure that designs.It is noted that alignment mark rod is preferably provided at ware body
Outward, because being once arranged in ware body, alignment mark rod easily produces impact to liquid level when rotating, and degree of regulation can be by certain
Impact, and it is unfavorable for that observation (if culture vessel is opaque, then can cause not observing, if culture vessel is transparent, see
Examine also not as align mark rod be arranged on ware external time convenient).
It is pointed out that cell to be cultivated can just be inserted culture vessel before adding culture fluid and (be needed to wait to cultivate
Cell of short duration can leave culture fluid and non-inactivation or death), now owing to there being the existence of cell filtration net, cell will not flow out
Culture vessel；Can also add at culture fluid, equal hydraulic control amount (i.e. carries out discharge opeing mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process) add cell to be cultivated afterwards；The culture fluid that band also can directly need to be cultivated cell is directly injected into training
(test under this premise should not consider the impact of initial cell quantity, and owing to having the existence of cell filtration net, cell to support vessel
Will not flow out culture vessel).Specifically can select according to test objective, demand.
Described liquid injection pipe upper end is provided with the lid 23 with liquid injection pipe threaded engagement, rotates and is arranged with for by the top on pipe
The vessel cover 24 that seals of the upper end open of a culture vessel.Lid can block and rotate pipe and extraneous connection, vessel cover
Can block a culture vessel of the top and extraneous connection, remaining culture vessel is then by a training nearest above self
Support vessel to block and extraneous connection.Such that it is able to ensure that each culture vessel separates with the external world in cell cultivation, process of the test,
Thus allow the present invention be applicable to the test being hedged off from the outer world under premise.
Described base includes matrix 1a and some support column 1b being arranged on below matrix, and matrix is provided with and runs through matrix
The end through hole 1c on upper and lower surface, rotates pipe lower end and stretches in end through hole, is provided with and opening up connects fluid cylinder 12 below end through hole,
Being provided with the bottom bracing plate 13 being connected with at least one support column below matrix, bottom bracing plate is provided with and connects cylinder adaptive bottom fluid cylinder
Groove, connects and is in bottom fluid cylinder in a groove.The liquid discharged from sump pit can be reclaimed (fall into and connect fluid cylinder) easily, can avoid wave
Take and avoid polluting to external world.
Described column includes cylinder and orifice plate 25, and cylinder lateral wall is provided with side opening, on a column: orifice plate is stuck in side opening
In, it being in sealing contact between orifice plate and side opening hole wall, orifice plate is made up of with lower half plate mutually symmetrical with upper half plate, upper half plate and lower half
Plate is formed in one structure, and equal liquid hole is positioned on upper half plate.In addition to equal liquid hole, orifice plate is laterally zygomorphic structure, consequently, it is possible to
One piece of orifice plate can be achieved with the Liquid level of two kinds of differing heights, i.e. by orifice plate with upper half plate upper and lower half plate under form
Snap in side opening, then through equal hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent)
After, liquid level consistent with equal liquid hole lowest part (now equal liquid hole is in more than in the middle part of orifice plate) in culture vessel；And by orifice plate
With upper half plate under, lower half plate snap in side opening in upper form, then liquid level and equal liquid hole lowest part in final culture vessel
Unanimously (now equal liquid hole is in the middle part of orifice plate following).Furthermore, it is possible to orifice plate is overlapped in configuration more, between each set orifice plate, only has equal liquid hole
Highly different, in so can be achieved with culture vessel, which liquid level is the quick control of the initial liquid measure of various differences (need, just
Use in a set of orifice plate corresponding with this liquid level is snapped in advance the side opening of each culture vessel).
Being provided with core pipe 4 and some horizontally disposed diaphragm plates 32 in described rotation pipe, core pipe lower end is stretched in end through hole, core
Being arranged with support ring 41 on pipe, support ring is higher than bottom core pipe and support ring is in end through hole, is provided with support seat in end through hole
14, support seat top and be provided with seat slot and the seat apical ring groove adaptive with rotating pipe lower end, contact seat slot bottom land bottom support ring, rotate
Contact seat apical ring groove bottom land in an apical ring groove is stretched in pipe lower end and bottom rotation pipe, rotate pipe and be rotatably assorted with supporting seat, core pipe
Being rotatably assorted through supporting seat and core pipe with supporting seat, diaphragm plate is set on core pipe, and diaphragm plate cross section is annular, rotates
Pipe, core pipe and each diaphragm plate are all coaxial, and core pipe can relatively rotate with diaphragm plate, is in sealing contact, tabula between core pipe and diaphragm plate
Seal fixing between plate and rotation pipe；
Diaphragm plate is provided with even lumen pore 321, and core pipe is provided with some hole sealing plates 42 for the company's of sealing lumen pore, hole sealing plate and tabula
Plate one_to_one corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, hole sealing plate bottom surface contact diaphragm plate
End face；
Rotate and form quantitative chamber 3a between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole 33, quantitative chamber
With pipe to fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: on column
Being provided with cylinder to fluid apertures 21, quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in
Cylinder, and the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder
To fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part；
Rotate pipe top be provided with and rotate the feed flow funnel 35 that pipe is threadeded.During the installation of each structure, cooperation, if feed flow
Funnel can interfere, then can first unload feed flow funnel, to be assembled, operated after, put in back feed flow funnel, and continue into
Row subsequent operation.
When needing to carry out each culture vessel mid-term while, liquid feeding (adds new culture fluid, Ke Yiyu in incubation
Initial incubation liquid kind, proportioning are identical, it is also possible to different from initial incubation liquid kind, proportioning) time, for each ware body, liquid
The time difference that body adds is the least, and exopathogenic factor interference suffered during final mensuration is the least (to be preferably capable of being simultaneously introduced cultivation
Liquid).But unless allow many people simultaneously to each culture vessel to carry out property while liquid feeding, and height to be ensured, can be only achieved more
Preferably effect, and in practical operation, the feasibility of this mode is on the low side.And in this programme, first turn rotates pipe, allow pipe
To fluid apertures with corresponding cylinder to stagger between fluid apertures (not connecting), then allow rotation pipe motionless, first turn core pipe (if rotate pipe with
Between column, frictional force is sufficiently large, then can direct turn core pipe, now rotating pipe will not be driven；Rub between pipe and column if rotating
Wiping power is limited, then can pinch rotation pipe on the other hand, another hands carrys out turn core pipe；The present invention design time, preferential former feelings
Shape), drive hole sealing plate leave even lumen pore (allow shrouding not company of sealing lumen pore), then can carry out liquid feeding from rotating pipe top, cultivation
Liquid through each even lumen pore, and can finally be full of all of quantitative chamber, then proceedes to make rotation pipe motionless, and turn core pipe makes envelope
Orifice plate seals even lumen pore, cuts off, and each quantitative intracavity liquid measure is consistent between the most each quantitative chamber.Then turn pipe can be rotated, logical
Cross diaphragm plate drive core pipe rotate together (diaphragm plate and to rotate between pipe be fixing, although can phase between core pipe and diaphragm plate
To rotation, but owing to being in sealing contact between core pipe and diaphragm plate, therefore between core pipe and diaphragm plate, necessarily there is the power of compression mutually
And frictional force, so if relatively rotating, need applying allow they external force in relative rotation.And only turn core pipe time, rubbing
Under the effect of wiping power, diaphragm plate can rotate together with core pipe, so rotating relative invariant position between Guan Yuxin pipe), allow
Pipe connects between fluid apertures to fluid apertures and corresponding cylinder, the most each quantitative chamber can give in corresponding ware body portion simultaneously, equivalent
Liquid feeding, can thoroughly eliminate the interference factors such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent during middle liquid feeding.This
Outward, this programme except can be used for liquid feeding in mid-term it can also be used to initially time liquid feeding.And after having had support seat, arrange from sump pit
The liquid gone out can first reach to support seat top, the most outwards flows down.
During it is noted that sump pit connects with equal liquid hole, pipe staggers between fluid apertures with corresponding cylinder to fluid apertures；Pipe is given
Fluid apertures is with corresponding cylinder to when connecting between fluid apertures, and sump pit staggers with equal liquid hole.And through equal hydraulic control amount (i.e. mentioned by all
Fluid apertures carries out discharge opeing so that process that in each ware body, liquid measure is consistent) after, in utilizing quantitative chamber to ware body, carry out the behaviour of liquid feeding
During work (when turn rotates pipe), it is impossible to allow sump pit again connect with equal liquid hole, otherwise can cause leakage at sump pit
(in ware body, liquid flows out from equal liquid hole, sump pit again), specific constructive form and mode of operation have a variety of, this measure two
Example,
Example 1: in the circumference rotating pipe, sump pit, equal liquid hole, pipe are arranged along up time pointing needle to fluid apertures successively to fluid apertures, cylinder.
All during hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent) operation, sump pit with
Rotation pipe and be clockwise to the equal liquid hole (during Gai, pipe connect to fluid apertures with corresponding cylinder to fluid apertures) that aligns, equal hydraulic control amount
After completing, sump pit continues to rotate clockwise and stagger with equal liquid hole, and now pipe does not still have to fluid apertures with corresponding cylinder to fluid apertures
Reaching connected state, if continuing turn clockwise subsequently to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures；
Example 2: in the circumference rotating pipe, equal liquid hole, sump pit, pipe are arranged to fluid apertures the most successively to fluid apertures, cylinder,
All during hydraulic control amount (i.e. carry out discharge opeing mentioned by equal liquid hole so that process that in each ware body, liquid measure is consistent) operation, sump pit with
Rotation pipe to rotate counterclockwise to alignment equal liquid hole (during Gai, pipe connect to fluid apertures with corresponding cylinder to fluid apertures), equal hydraulic control amount
After completing, sump pit rotates clockwise and staggers with equal liquid hole, and now pipe is still not reaching to fluid apertures with corresponding cylinder to fluid apertures
Connected state, if continuing turn clockwise subsequently to rotate pipe, can allow pipe connect to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber with culture vessel: quantitatively intracavity is provided with tune amount liquid capsule 34, tune amount liquid capsule be provided with for
To the feed pipe of tune amount liquid capsule feed flow, feed pipe one end connects with tune amount liquid capsule, and feed pipe is by the hard tube section being in quantitative intracavity
And the outer segment being in outside quantitative chamber is constituted；
Base is provided with some for the confession fluid cylinder 5 of feed pipe feed flow, including that cylinder body 5a joins with cylinder body slipper seal for fluid cylinder
The feed flow piston 5b that closes and be connected with feed flow piston and be in the piston rod 5c above feed flow piston, feed flow piston slidably just
To for above-below direction, in one for fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity 5d, in cylinder body on feed flow piston
The space of side is regulation chamber 5e；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
Described base is provided with the adjustment seat 6 fixing with base, is provided with horizontally disposed adjustable plate 61 below adjustment seat, lives
Stopper rod passes adjustable plate, and piston rod is provided with upper holder block 611 and lower drawing block 612, and adjustment seat is provided with vertically arranges and through adjusting
Saving the homophony pole 62 of seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjustable plate end face, lower drawing block end face contact adjustable plate bottom surface, main regulation masthead portion is provided with fixing with homophony pole
Main regulation knob 621.
Described homophony pole is provided with the instruction dish 622 being in above adjustment seat, and instruction dish is coaxial with homophony pole, regulation
Seat is provided with the indicating arm 63 of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction dish sidewall.
As it was previously stated, the present invention can carry out equivalent, simultaneously liquid feeding in each ware body.And in this programme, it is also possible to right
Liquid volume added regulates and controls.At the beginning, tune amount liquid capsule is vacuum, if now specified rate chamber is full of, then liquid volume added is exactly the reality in quantitative chamber
Border volume.When needs control liquid volume added, homophony pole can be made to move up or down with turn homophony pole (main regulation knob)
Dynamic, as a example by moving down: now homophony pole drives adjustable plate vertically to move down (upper holder block by the upper holder block on homophony pole
Rotating, adjustable plate does not turns), adjustable plate pushes down on each feed flow piston, the liquid in each sap cavity by each lower drawing block on piston rod
Body (can be water or other liquid, if in stable condition, the structures such as the pipeline of process will not be damaged) inject right
The tune amount liquid capsule answered, now, the actual volume (actual volume refers to accommodate the volume of culture fluid) in quantitative chamber diminishes,
And the numerical value specifically diminished, can read from indicating arm (the scale groove on indicating arm can mark actual with quantitative chamber hold
Long-pending corresponding numeral, the occurrence of numeral, when manufacturing and designing, can convert with measured in advance.Bid can be done on instruction dish
Note, it is simple to directly carry out, according to labelling, the reading that mark-sense is alignd, or, instruction dish can also make as labelling itself
With, as utilized instruction dish end face or bottom surface to be used as labelling).The actual volume in quantitative chamber can be diminished by said process, thus
Control liquid volume added (making it reduce)；Otherwise, allow homophony pole move up, then tune can be measured the liquid in liquid capsule and extract out, thus
Liquid volume added (making it increase) can be controlled.It is noted that feed pipe is by being in the hard tube section of quantitative intracavity and being in outside quantitative chamber
Outer segment constitute, wherein outer segment can not be perfect rigidity, to guarantee that diaphragm plate, core pipe etc. can normal rotation (core pipes
Being connected with outer segment, when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity), outer segment can
To select the pipe of the type such as sebific duct, oil cylinder petroleum pipeline.
It is provided with the sealing depression bar 7 of vertically layout in described rotation pipe, seals depression bar and through all diaphragm plates and seal depression bar
All threaded with between all diaphragm plates, seal depression bar and be provided with some pressure strips 71 for compressing hole sealing plate, pressure strip
With hole sealing plate one_to_one corresponding, pressure strip is in the top of corresponding hole sealing plate.As it was previously stated, fill it up with liquid in rotation pipe, then seal
Orifice plate seals after connecting lumen pore, cuts off between each quantitative chamber.But, in order to allow hole sealing plate want can the thoroughly company's of sealing lumen pore, hole sealing plate
And the extruding mutually between one piece of diaphragm plate of lower contact is inevitable bigger.After being used for multiple times, easily cause because abrasion is relatively big
Seal deficiency.And in this programme, hole sealing plate seals after connecting lumen pore, it is also possible to turn seals depression bar, pressure strip is driven to move down and press
Tight corresponding hole sealing plate, improves sealing further, ensures the partition effect between each quantitative chamber.And had sealing depression bar, pressure
After tight plate, during fabrication, need not press between hole sealing plate and diaphragm plate too tight (sealing can be protected by the pressure down of pressure strip
Barrier), such that it is able to effectively reduce abrasion, increase the service life.It is noted that after having had sealing depression bar, pressure strip, turn allowing
Dynamic pipe is motionless and before turn core pipe, pressure strip should be allowed to be not located at compressing the state of corresponding hole sealing plate, otherwise there will be " turning motionless "
Situation.
Claims (7)
1. a multisample culture apparatus, is characterized in that, including base and the culture vessel of some upper end open, each culture vessel
Stacking gradually from the bottom to top, a culture vessel contact base of bottom, described culture vessel includes ware body, is arranged on ware body
Column on inner bottom part, the projection being arranged on the external bottom of ware and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout,
Projection cross section is rectangular, and ware body top end face is provided with the ware inverted draw cut adaptive with projection, and base top is provided with adaptive with projection
Base inverted draw cut, the projection of a culture vessel of bottom is in base inverted draw cut, the equal opening of column upper/lower terminal, a vertical cloth
The rotation pipe put can relatively rotate through all columns, described rotation pipe with column, and rotates pipe lateral wall and column medial wall
Between be in sealing contact, rotate pipe lateral wall be provided with runs through rotate pipe upper/lower terminal sump pit；
In a culture vessel: be provided with the equal liquid hole connected with portion in ware body on column, be provided with cell filtration net in equal liquid hole,
Liquid injection pipe lower end connects with portion in ware body, and ware body sidewall is provided with the tune amount transverse tube connected with portion in ware body, and tune amount transverse tube sets
Having the tune amount post running through tune two pipe ends of amount transverse tube, tune amount post is threadeded with tune amount transverse tube, and tune amount post is same with tune amount transverse tube
Axle, tune amount post the inner is in ware body, and tune amount post outer end is in outside tune amount transverse tube, and tune amount post sidewall is provided with baseline trough and is in
Alignment mark rod outside tune amount transverse tube, tune amount transverse tube is provided with baseline rod；
In adjacent two culture vessels: in the ware inverted draw cut of the projection of top culture vessel culture vessel at the bottom；
Described column includes cylinder and orifice plate, and cylinder lateral wall is provided with side opening, on a column: orifice plate is stuck in side opening, hole
Being in sealing contact between plate and side opening hole wall, orifice plate is made up of with lower half plate mutually symmetrical with upper half plate, and upper half plate with lower half plate is
Integrated formed structure, equal liquid hole is positioned on upper half plate；
Described liquid injection pipe upper end is provided with and the lid of liquid injection pipe threaded engagement, rotates and is arranged with for by the one of the top on pipe
The vessel cover that the upper end open of culture vessel seals.
A kind of multisample culture apparatus the most according to claim 1, is characterized in that, described base includes matrix and some sets
Putting the support column below matrix, matrix is provided with the end through hole running through the upper and lower surface of matrix, rotates pipe lower end and stretches into end through hole
In, it is provided with the opening up fluid cylinder that connects below end through hole, below matrix, is provided with the bottom bracing plate being connected with at least one support column, the end
Fagging is provided with and connects cylinder groove adaptive bottom fluid cylinder, connects and is in bottom fluid cylinder in a groove.
A kind of multisample culture apparatus the most according to claim 1, is characterized in that, if described rotation pipe is provided with core pipe and
Dry horizontally disposed diaphragm plate, core pipe lower end is stretched in end through hole, and core pipe is arranged with support ring, and support ring is higher than bottom core pipe
And support ring is in end through hole, in end through hole, it is provided with support seat, supports seat top and be provided with seat slot and adaptive with rotating pipe lower end
Seat apical ring groove, contact seat slot bottom land bottom support ring, rotate pipe lower end and stretch in an apical ring groove and rotate and bottom pipe, contact seat
Apical ring groove bottom land, rotates pipe and is rotatably assorted with supporting seat, and core pipe is rotatably assorted through support seat and core pipe with supporting seat, diaphragm plate
Being set on core pipe, diaphragm plate cross section is annular, rotates pipe, core pipe and each diaphragm plate all coaxial, and core pipe and diaphragm plate can
Relatively rotate, be in sealing contact between core pipe and diaphragm plate, seal fixing between diaphragm plate and rotation pipe；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate: hole sealing plate is in above diaphragm plate, and contact diaphragm plate top, hole sealing plate bottom surface
Face；
Rotate and form quantitative chamber between the interior adjacent two pieces of diaphragm plates of pipe, if rotating pipe to be provided with main feeding hole, quantitative chamber and pipe
To fluid apertures one_to_one corresponding, quantitative chamber and culture vessel one_to_one corresponding, in corresponding quantitative chamber with culture vessel: be provided with on column
Fluid apertures given by cylinder, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe to fluid apertures and cylinder to fluid apertures all in cylinder,
And the pipe interconnected is coaxial to fluid apertures with cylinder to fluid apertures, cylinder feeding axially bored line level, quantitative chamber lowest part is higher than cylinder to fluid apertures
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
A kind of multisample culture apparatus the most according to claim 3, is characterized in that, in corresponding quantitative chamber and culture vessel
In: quantitatively intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule is provided with for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end with
Tune amount liquid capsule connects, and feed pipe is made up of the hard tube section being in quantitative intracavity and the outer segment that is in outside quantitative chamber；
Base is provided with some for the confession fluid cylinder of feed pipe feed flow, including that cylinder body coordinates with cylinder body slipper seal for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, at one in fluid cylinder: in cylinder body, the space below feed flow piston is sap cavity, space above feed flow piston in cylinder body
For regulation chamber；
Sap cavity and feed pipe one_to_one corresponding, the feed pipe other end connects with corresponding sap cavity, feed pipe pass rotation tube side wall and
Rotate pipe lower end, seal fixing between feed pipe and rotation tube side wall.
A kind of multisample culture apparatus the most according to claim 4, is characterized in that, described base is provided with to be fixed with base
Adjustment seat, be provided with horizontally disposed adjustable plate below adjustment seat, piston rod pass adjustable plate, piston rod be provided with upper holder block and
Lower drawing block, adjustment seat is provided with the homophony pole vertically arranging and passing adjustment seat, and homophony pole is threadeded with adjustment seat, main
Adjusting rod is provided with upper holder block and lower drawing block, upper holder block bottom surface contact adjustable plate end face, and lower drawing block end face contacts adjustable plate bottom surface,
Main regulation masthead portion is provided with the main regulation knob fixing with homophony pole.
A kind of multisample culture apparatus the most according to claim 5, is characterized in that, described homophony pole is provided with and is in tune
Instruction dish above joint seat, instruction dish is coaxial with homophony pole, and adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertical
Arrange and indicating arm contact instruction dish sidewall.
7. according to a kind of multisample culture apparatus described in Claims 2 or 3 or 4, it is characterized in that, be provided with perpendicular in described rotation pipe
The straight sealing depression bar arranged, seals depression bar and all threadeds with all diaphragm plates through all diaphragm plates and sealing depression bar,
Sealing depression bar and be provided with some pressure strips for compressing hole sealing plate, pressure strip and hole sealing plate one_to_one corresponding, pressure strip is in right
Answer the top of hole sealing plate.
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| CN201610701445.1A CN106190843B (en) | 2016-08-22 | 2016-08-22 | A kind of multisample culture apparatus |
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| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
| CN107711304A (en) * | 2017-10-31 | 2018-02-23 | 四川明峰农业开发有限公司 | One plant stereo cultivation apparatus |
| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
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| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
| CN106222090B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment assistant carrier |
| CN106467893B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment auxiliary device |
| CN106244457B (en) * | 2016-08-22 | 2018-08-03 | 日照安维信生物科技有限公司 | A kind of experiment culture carrier structure |
| CN106047703B (en) * | 2016-08-22 | 2018-08-10 | 青岛爱博生物科技有限公司 | A kind of test sample culture apparatus |
| CN107711304A (en) * | 2017-10-31 | 2018-02-23 | 四川明峰农业开发有限公司 | One plant stereo cultivation apparatus |
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