CN205933844U - Multilayer formula cell breeding device - Google Patents
Multilayer formula cell breeding device Download PDFInfo
- Publication number
- CN205933844U CN205933844U CN201620929362.3U CN201620929362U CN205933844U CN 205933844 U CN205933844 U CN 205933844U CN 201620929362 U CN201620929362 U CN 201620929362U CN 205933844 U CN205933844 U CN 205933844U
- Authority
- CN
- China
- Prior art keywords
- pipe
- cylinder
- plate
- hole
- fluid apertures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a multilayer formula cell breeding device, including base and a plurality of cultivation household utensils, each is trained household utensils and stacks gradually, trains household utensils and includes dish body, vertical cylinder and annotate the liquid pipe, and the equal opening in the upper and lower both ends of vertical cylinder, a swinging head pass all vertical cylinder, and the swinging head can rotate with vertical cylinder relatively, and sealing contact between swinging head and the vertical cylinder, is equipped with the displacement tank on the swinging head lateral wall, in cultivation household utensils: vertical cylinder is last be equipped with with the equal liquid hole of the inside intercommunication of dish body, downthehole cell filtration net and the equal liquid check valve of being equipped with of equal liquid, equal liquid check valve is in between cell filtration net and the swinging head, the accessible orientation of equal liquid check valve be by in the dish body outside dish body, annotate liquid pipe lower extreme and dish body is inside feeds through. The beneficial effects of the utility model are that: be applicable to cell culture and survey, compare, the reasonable and stable in structure of spatial layout, operation process is comparatively convenient, and regulation uniformity in the process of the test is good, the accuracy is high.
Description
Technical field
This utility model belongs to technical field of cell culture, more particularly, to a kind of multiple field cell culture device.
Background technology
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology.The life of cell
Length needs nutrient environment, and the nutrient matrix for maintaining cell growth is referred to as culture medium.Culture medium can be divided into by its physical state
Fluid medium and solid medium.Cell culture technology, in addition to being applied to produce, is more for testing at present（As right
Group sample parameter is measured, and compares, analyzes and study）, and test in the more commonly used to be fluid medium.This
Outward, cell culture needs to use cell culture system, common are the cell culture under culture dish, incubator etc., general environment
Can be directly using culture dish, if desired for some special environments（As energising, heating etc.）, then special incubator can be used.
At present carry out cell culture and during Comparability test when although common culture apparatuses can play and carry for culture medium
For the function of bearing space, but at aspects such as space layout reasonability, integrated operation convenience, regulating effect concordance, still have
It is short of.
Utility model content
This utility model is to overcome deficiency of the prior art, there is provided one kind be applied to cell culture, mensure and
Analyse and compare, rationally and Stability Analysis of Structures, operating process is more convenient, and the regulation concordance in process of the test is good, accurate for space layout
The really high breeding apparatus of property.
To achieve these goals, this utility model employs the following technical solutions：
A kind of multiple field cell culture device, including the culture vessel of base and some upper end open, each culture vessel by
Under supreme stack gradually, culture vessel contact base of bottom, described base is provided with fixing vertical rod, outside culture vessel
The retaining collar that side wall is provided with and fixing vertical rod is adapted to, retaining collar is set in fixing vertical rod, culture vessel include ware body,
The column being arranged on bottom in ware body and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, column upper/lower terminal is equal
Opening, through all columns, described rotation pipe and column can relatively rotate a rotation pipe vertically arranged, and rotate outside pipe
In sealing contact between wall and column medial wall, rotate pipe lateral wall and be provided with and run through the sump pit rotating pipe upper/lower terminal, one
In individual culture vessel：Column is provided with the equal liquid hole connecting with portion in ware body, is provided with cell filtering net, under liquid injection pipe in equal liquid hole
End is connected with portion in ware body.
Preferably, described liquid injection pipe upper end be provided with fluid injection pipe screw thread cooperation lid, rotate pipe on be arranged with for
The vessel cover that the upper end open of one culture vessel of the top is sealed.
Preferably, described base includes matrix and some support columns being arranged on below matrix, matrix is provided with and runs through
The bottom through hole on the upper and lower surface of matrix, rotates pipe lower end and stretches in the through hole of bottom, be provided with below the through hole of bottom opening up connect fluid cylinder,
It is provided with the bottom bracing plate being connected with least one support column, bottom bracing plate is provided with and connects the cylinder groove that fluid cylinder bottom is adapted to below matrix,
Connect fluid cylinder bottom to be in a groove.
Preferably, being provided with core pipe and some horizontally disposed diaphragm plates in described rotation pipe, core pipe lower end is stretched into bottom and is led to
In the hole, core pipe is arranged with support ring, and support ring is higher than core pipe bottom and support ring is in the through hole of bottom, is provided with and props up in the through hole of bottom
Support seat, support base top is provided with seat slot and the annular groove being adapted to rotation pipe lower end, and support ring bottom contacts seat slot bottom land, rotates pipe
Lower end is stretched in annular groove and is rotated bottom of the tube contact annular groove bottom land, rotates pipe and is rotatably assorted with support base, core pipe passes through support base
And core pipe is rotatably assorted with support base, diaphragm plate is set in core pipe, diaphragm plate cross section be in annular, rotate pipe, core pipe and
Each diaphragm plate is all coaxial, and core pipe can relatively rotate with diaphragm plate, in sealing contact between core pipe and diaphragm plate, and diaphragm plate is managed with rotating
Between sealing fix；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and tabula
Plate corresponds, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts diaphragm plate
Top surface；
Rotate in pipe and between adjacent two pieces of diaphragm plates, form quantitative chamber, if rotating pipe to be provided with main to fluid apertures, quantitative chamber
Correspond to fluid apertures with pipe, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：On column
It is provided with cylinder to fluid apertures, quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, pipe is all in justify with cylinder to fluid apertures to fluid apertures
Cylindricality, and the pipe interconnecting is coaxial to fluid apertures with cylinder to fluid apertures, cylinder is given higher than cylinder to fluid apertures axis horizontal, quantitative chamber lowest part
Fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
Preferably, in corresponding quantitative chamber with culture vessel：Quantitative intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule sets
Have for the feed pipe to tune amount liquid capsule feed flow, feed pipe one end is connected with tune amount liquid capsule, feed pipe is by being in quantitative intracavity
Hard tube section and the outer segment being in outside quantitative chamber are constituted；
Base be provided with some for the confession fluid cylinder to feed pipe feed flow, include cylinder body and cylinder body slipper seal for fluid cylinder
Cooperation feed flow piston and be connected and be in the piston rod above feed flow piston, the slidably direction of feed flow piston with feed flow piston
For above-below direction, supply in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity, above feed flow piston in cylinder body
Space is to adjust chamber；
Sap cavity is corresponded with feed pipe, and the feed pipe other end is connected with corresponding sap cavity, and feed pipe passes through and rotates tube side
Wall and rotation pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
Preferably, described base is provided with the adjustment seat fixing with base, below adjustment seat, it is provided with horizontally disposed tune
Section plate, piston rod passes through adjustable plate, and piston rod is provided with upper holder block and lower drawing block, and adjustment seat is provided with vertically to arrange and pass through and adjusts
The homophony pole of section seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block bottom
Face contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, and main regulation masthead portion is provided with the master fixing with homophony pole
Adjusting knob.
Preferably, described homophony pole is provided with the instruction disk being in above adjustment seat, instruction disk is same with homophony pole
Axle, adjustment seat is provided with the indicating arm of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction Pan Cebi.
Preferably, described rotate the sealing depression bar being provided with vertical arrangement in pipe, sealing depression bar pass through all diaphragm plates and
All threaded between sealing depression bar and all diaphragm plates, sealing depression bar is provided with some pressure strips for compressing hole sealing plate,
Pressure strip is corresponded with hole sealing plate, and pressure strip is in the top of corresponding hole sealing plate.
The beneficial effects of the utility model are：It is applied to cell culture and mensure, comparison, space layout is reasonable and structure is steady
Fixed, operating process is more convenient, and the regulation concordance in process of the test is good, accuracy is high.
Brief description
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of this utility model orifice plate；
Fig. 3 is the enlarged drawing at A in Fig. 1；
Fig. 4 is the enlarged drawing at B in Fig. 1；
Fig. 5 is the enlarged drawing at C in Fig. 1；
Fig. 6 is the sectional view that this utility model rotates at pipe.
In figure：Base 1, fixing vertical rod 11, connect fluid cylinder 12, bottom bracing plate 13, support base 14, matrix 1a, support column 1b, bottom lead to
Hole 1c, culture vessel 2, retaining collar 21, equal liquid hole 22, lid 23, vessel cover 24, orifice plate 25, cylinder to fluid apertures 26, ware body 2a,
Column 2b, liquid injection pipe 2c, rotate pipe 3, sump pit 31, diaphragm plate 32, even lumen pore 321, pipe be to fluid apertures 33, tune amount liquid capsule 34, fixed
Measure chamber 3a, core pipe 4, support ring 41, hole sealing plate 42, supply fluid cylinder 5, cylinder body 5a, feed flow piston 5b, piston rod 5c, sap cavity 5d, regulation
Chamber 5e, adjustment seat 6, adjustable plate 61, upper holder block 611, lower drawing block 612, homophony pole 62, main regulation knob 621, instruction disk 622,
Indicating arm 63, sealing depression bar 7, pressure strip 71.
Specific embodiment
With reference to the accompanying drawings and detailed description this utility model is further described.
In embodiment as shown in Figures 1 to 6, a kind of multiple field cell culture device, open including base 1 and some upper ends
The culture vessel 2 of mouth, each culture vessel stacks gradually from the bottom to top, a culture vessel contact base of bottom, described bottom
Seat is provided with fixing vertical rod 11, and culture vessel lateral wall is provided with the retaining collar 21 being adapted to fixing vertical rod, fixed cover ring set
It is located in fixing vertical rod, culture vessel includes ware body 2a, the column 2b being arranged on bottom in ware body and is arranged on ware body sidewall
Liquid injection pipe 2c that is upper and being in tilted layout, the equal opening of column upper/lower terminal, a rotation pipe 3 vertically arranged passes through all columns,
The described pipe that rotates can relatively rotate with column, and rotates in sealing contact between pipe lateral wall and column medial wall, rotates outside pipe
Wall is provided with and runs through the sump pit 31 rotating pipe upper/lower terminal, in a culture vessel：Column is provided with portion in ware body even
Logical equal liquid hole 22, is provided with cell filtering net in equal liquid hole, liquid injection pipe lower end is connected with portion in ware body.
Retaining collar is made to be inserted in when before test, from the bottom to top each culture vessel can be stacked gradually placement, and place solid
Determine vertical rod, so that column is covered to outside rotation pipe（The structure rotating in pipe and rotation pipe can pre-set）, so that each cultivate
Vessel can align, and the culture vessel of top just covers the culture vessel of lower section.Then liquid feeding is carried out to each liquid injection pipe（Plus
Enter fluid medium or culture fluid）, deliberately need not control liquid volume added during liquid feeding, as long as liquid level lid crosses equal liquid hole.All add
After the completion of liquid, turn rotates pipe, allows sump pit to connect with each equal liquid hole, so that the liquid in each culture vessel can be from equal liquid
Hole is flowed out, and in culture vessel, remaining liquid measure reaches unanimously, subsequently turn can rotate pipe, allow sump pit leave equal liquid hole（Make it
Do not connect）, so just it has been rapidly completed liquid feeding.
It is pointed out that treating that cultured cells can just insert culture vessel before adding culture fluid（Need to wait to cultivate
Cell of short duration can leave culture fluid and non-inactivation or death）, now due to there being the presence of cell filtering net, cell will not flow out
Culture vessel；Can also add in culture fluid, equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that liquid in each ware body
Measure consistent process）Add afterwards and treat cultured cells；Also the culture fluid that directly band can be needed cultured cells is directly injected into training
Foster vessel（Test under the premise of being somebody's turn to do should not consider the impact of initial cell quantity, and due to having the presence of cell filtering net, cell
Will not flow out culture vessel）.Specifically can be selected according to test objective, demand.
Described liquid injection pipe upper end is provided with the lid 23 with the cooperation of fluid injection pipe screw thread, rotates and is arranged with for by the top on pipe
A culture vessel the vessel cover 24 that seals of upper end open.Lid can block and rotate pipe and extraneous connection, vessel cover
A culture vessel of the top and extraneous connection can be blocked, remaining culture vessel is then by a nearest training above itself
Foster vessel block and extraneous connection.Such that it is able to ensure that each culture vessel separated with the external world in cell culture, process of the test,
Thus the test under the premise of allowing this utility model to be applicable to be hedged off from the outer world.
Described base includes matrix 1a and some support column 1b being arranged on below matrix, and matrix is provided with and runs through matrix
The bottom through hole 1c on upper and lower surface, rotates pipe lower end and stretches in the through hole of bottom, be provided with below the through hole of bottom opening up connect fluid cylinder 12,
It is provided with the bottom bracing plate 13 being connected with least one support column, bottom bracing plate is provided with and connects the cylinder that fluid cylinder bottom is adapted to below matrix
Groove, connects fluid cylinder bottom and is in a groove.The liquid discharged from sump pit can be reclaimed easily（Fall into and connect fluid cylinder）, wave can be avoided
Take and avoid polluting to external world.
Described column includes cylinder and orifice plate 25, and cylinder lateral wall is provided with side opening, on a column：Orifice plate is stuck in side opening
Interior, in sealing contact between orifice plate and side opening hole wall, orifice plate is made up of mutually symmetrical with upper half plate and lower half plate, upper half plate and lower half
Plate is formed in one structure, and equal liquid hole is located on upper half plate.In addition to equal liquid hole, orifice plate is laterally zygomorphic structure, consequently, it is possible to
One piece of orifice plate can be achieved with the Liquid level of two kinds of differing heights, i.e. by orifice plate with upper half plate upper and lower half plate under in the form of
Snap in side opening, then through equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）
Afterwards, in culture vessel, liquid level is consistent with equal liquid hole lowest part（Now equal liquid hole is in more than in the middle part of orifice plate）；And by orifice plate
With upper half plate under, lower half plate snap in side opening in upper form, then liquid level and equal liquid hole lowest part in final culture vessel
Unanimously（Now equal liquid hole is in below in the middle part of orifice plate）.Furthermore, it is possible to configure many set orifice plates, between each set orifice plate, only has equal liquid hole
Highly different, so can be achieved with the quick control of various different initial liquid measures in culture vessel（Needed which liquid level, just
A set of orifice plate corresponding with this liquid level is snapped in advance and is used in the side opening of each culture vessel）.
It is provided with core pipe 4 and some horizontally disposed diaphragm plates 32, core pipe lower end is stretched in the through hole of bottom, core in described rotation pipe
Support ring 41 is arranged with pipe, support ring is higher than core pipe bottom and support ring is in the through hole of bottom, is provided with support base in the through hole of bottom
14, support base top is provided with seat slot and the annular groove being adapted to rotation pipe lower end, and support ring bottom contacts seat slot bottom land, rotates under pipe
End is stretched in annular groove and is rotated bottom of the tube contact annular groove bottom land, rotates pipe and is rotatably assorted with support base, core pipe through support base and
Core pipe is rotatably assorted with support base, and diaphragm plate is set in core pipe, and diaphragm plate cross section is in annular, rotates pipe, core pipe and each
Diaphragm plate is all coaxial, core pipe can relatively rotate with diaphragm plate, in sealing contact between core pipe and diaphragm plate, diaphragm plate with rotate pipe
Between sealing fix；
Diaphragm plate is provided with even lumen pore 321, and core pipe is provided with some hole sealing plates 42 for company of sealing lumen pore, hole sealing plate and
Diaphragm plate corresponds, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and the contact of hole sealing plate bottom surface is horizontal
Dividing plate top surface；
Rotate in pipe and between adjacent two pieces of diaphragm plates, form quantitative chamber 3a, if rotating pipe to be provided with main to fluid apertures 33, fixed
Amount chamber and pipe correspond to fluid apertures, and quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：Perpendicular
Cylinder is provided with cylinder to fluid apertures 26, and quantitative chamber is communicated to cylinder to fluid apertures by corresponding pipe to fluid apertures, and pipe is to fluid apertures and cylinder to fluid apertures
All in cylinder, and the pipe interconnecting is coaxial to fluid apertures with cylinder to fluid apertures, and, to fluid apertures axis horizontal, quantitative chamber lowest part is high for cylinder
In cylinder to fluid apertures lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
When needs carry out mid-term liquid feeding simultaneously to each culture vessel（New culture fluid, Ke Yiyu is added in incubation
Initial incubation liquid species, proportioning identical it is also possible to different from initial incubation liquid species, proportioning）When, for each ware body, liquid
The time difference that body adds is less, and during final mensure, the interference of suffered exopathogenic factor is also less（It is preferably capable of being simultaneously introduced culture
Liquid）.But unless allowed many people to carry out liquid feeding to each culture vessel simultaneously, and height while property to be ensured, can be only achieved more
Preferably effect, and in practical operation, the feasibility of this mode is low.And in this programme, first turn rotates pipe, allow pipe
Stagger between fluid apertures with corresponding cylinder to fluid apertures（Do not connect）, then allow rotation manage motionless, first turn core pipe（If rotate pipe with
Between column, frictional force is sufficiently large, then can direct turn core pipe, now rotating pipe will not be driven；If rotate rubbing between pipe and column
Wiping power is limited, then can pinch rotation pipe on the other hand, and another handss carry out turn core pipe；This utility model is in design, preferentially previous
Plant situation）, drive hole sealing plate to leave even lumen pore（Allow the shrouding not company's of sealing lumen pore）, then can carry out liquid feeding from rotating pipe top,
Culture fluid through each even lumen pore, and finally can be full of all of quantitative chamber, then proceedes to allow rotation manage motionless, turn core pipe,
Make hole sealing plate seal even lumen pore, separate between now each quantitative chamber, and each quantitative intracavity liquid measure is consistent.Then can turn rotate
Pipe, drives core pipe to rotate together by diaphragm plate（It is fixing between diaphragm plate and rotation pipe, although between core pipe and diaphragm plate
Can relatively rotate, but due in sealing contact between core pipe and diaphragm plate, therefore between core pipe and diaphragm plate, necessarily have mutual compression
Power and frictional force, so if will relatively rotating, need the external force applying to allow them to relatively rotate.And only turn core pipe when,
In the presence of frictional force, diaphragm plate can rotate together with core pipe, thus rotate pipe and core pipe between relative position not
Become）, allow pipe to connect between fluid apertures with corresponding cylinder to fluid apertures, now each quantitative chamber can be same to portion in corresponding ware body
When, equivalent liquid feeding, can thoroughly eliminate during middle liquid feeding the interference such as the liquid feeding time started is inconsistent, liquid feeding duration is inconsistent because
Element.Additionally, this programme except can be used for mid-term liquid feeding it can also be used to initially when liquid feeding.And after having support base, from discharge opeing
The liquid that groove is discharged can first reach support base top, then outwards flows down.
It is noted that when sump pit is connected with equal liquid hole, pipe is staggered between fluid apertures with corresponding cylinder to fluid apertures；Manage to
To between fluid apertures, during connection, sump pit is staggered fluid apertures with equal liquid hole with corresponding cylinder.And pass through equal hydraulic control amount（I.e. mentioned by equal
Fluid apertures carries out discharge opeing so that the consistent process of liquid measure in each ware body）Afterwards, carry out the behaviour of liquid feeding in using quantitative chamber to ware body
During work（When turn rotates pipe）It is impossible to allow sump pit to connect again with equal liquid hole, leakage at sump pit otherwise can be led to
（In ware body, liquid flows out from equal liquid hole, sump pit again）, specific constructive form and mode of operation have many kinds, in this measure two
Example,
Example 1：In the circumference rotating pipe, sump pit, equal liquid hole, pipe are to fluid apertures, cylinder to fluid apertures along up time pointing needle successively
Arrangement.All hydraulic control amounts（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath is clockwise to alignment equal liquid hole with rotating pipe（During being somebody's turn to do, pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）, all
After the completion of hydraulic control amount, sump pit is continued to rotate clockwise and is staggered with equal liquid hole, now pipe to fluid apertures and corresponding cylinder to fluid apertures according to
So being not reaching to connected state, if subsequently continuing turn clockwise to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures；
Example 2：In the circumference rotating pipe, equal liquid hole, sump pit, pipe are to fluid apertures, cylinder to fluid apertures along clockwise direction successively
Arrangement, equal hydraulic control amount（Carry out discharge opeing mentioned by equal liquid hole so that the consistent process of liquid measure in each ware body）During operation, row
Liquid bath rotates counterclockwise to alignment equal liquid hole with rotating pipe（During being somebody's turn to do, pipe is not connected to fluid apertures with corresponding cylinder to fluid apertures）, all
After the completion of hydraulic control amount, sump pit is rotated clockwise and is staggered with equal liquid hole, and now pipe is not still had to fluid apertures with corresponding cylinder to fluid apertures
Having and reach connected state, if subsequently continuing turn clockwise to rotate pipe, pipe can be allowed to connect to fluid apertures with corresponding cylinder to fluid apertures.
In corresponding quantitative chamber with culture vessel：Quantitative intracavity is provided with tune amount liquid capsule 34, tune amount liquid capsule be provided with for
To the feed pipe of tune amount liquid capsule feed flow, feed pipe one end is connected with tune amount liquid capsule, and feed pipe is by the hard tube section being in quantitative intracavity
And the outer segment being in outside quantitative chamber is constituted；
Base be provided with some for the confession fluid cylinder 5 to feed pipe feed flow, for fluid cylinder include cylinder body 5a and cylinder body slide close
The feed flow piston 5b of envelope cooperation and be connected and be in the piston rod 5c above feed flow piston with feed flow piston, the sliding of feed flow piston
Dynamic direction is above-below direction, supplies in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity 5d, and in cylinder body, feed flow is lived
The space of side is to adjust chamber 5e beyond the Great Wall；
Sap cavity is corresponded with feed pipe, and the feed pipe other end is connected with corresponding sap cavity, and feed pipe passes through and rotates tube side
Wall and rotation pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
Described base is provided with the adjustment seat 6 fixing with base, is provided with horizontally disposed adjustable plate 61 below adjustment seat, lives
Stopper rod passes through adjustable plate, and piston rod is provided with upper holder block 611 and lower drawing block 612, and adjustment seat is provided with vertical arrangement and through tune
The homophony pole 62 of section seat, homophony pole is threadeded with adjustment seat, and homophony pole is provided with upper holder block and lower drawing block, upper holder block
Bottom surface contact adjusts plate top surface, and lower drawing block top surface contacts adjustable plate bottom surface, and main regulation masthead portion is provided with fixing with homophony pole
Main regulation knob 621.
Described homophony pole is provided with the instruction disk 622 being in above adjustment seat, and instruction disk is coaxial with homophony pole, adjusts
Seat is provided with the indicating arm 63 of groove with a scale, and indicating arm is vertically arranged and indicating arm contact instruction Pan Cebi.
As it was previously stated, this utility model can carry out equivalent, simultaneously liquid feeding in each ware body.And in this programme, also may be used
To regulate and control to liquid volume added.At the beginning, tune amount liquid capsule is vacuum, if now specified rate chamber is full of, liquid volume added is exactly quantitative chamber
Actual volume.When needing to control liquid volume added, can be with turn homophony pole（Main regulation knob）, make homophony pole upwards or to
Lower movement, taking move down as a example：The upper holder block that now homophony pole passes through on homophony pole drives adjustable plate vertically to move down（On
Briquetting rotates, and adjustable plate does not turn）, adjustable plate pushes down on each feed flow piston by each lower drawing block on piston rod, in each sap cavity
Liquid（Can be water or other liquid, as long as in stable condition, the structures such as the pipeline passing through will not be damaged）Note
Enter corresponding tune amount liquid capsule, now, the actual volume in quantitative chamber（Actual volume refers to accommodate the volume of culture fluid）Become
Little, and the numerical value specifically diminishing, can read from indicating arm（Scale groove on indicating arm can mark real with quantitative chamber
Numeral corresponding to the volume of border, the occurrence of numeral, when manufacturing and designing, can be converted with measured in advance.Can do on instruction disk
Go out labelling, be easy to the reading directly being alignd come mark-sense according to labelling, or, instruction disk itself can also come as labelling
Use, be such as used as labelling using instruction disk top surface or bottom surface）.The actual volume in quantitative chamber can be diminished by said process, from
And control liquid volume added（So that it is reduced）；Conversely, allowing homophony pole to move up, then the liquid in tune amount liquid capsule can be extracted out, from
And can control liquid volume added（So that it is increased）.It is noted that feed pipe by the hard tube section being in quantitative intracavity and is in quantitative chamber
Outer outer segment is constituted, and wherein outer segment can not be perfect rigidity, to guarantee that diaphragm plate, core pipe etc. being capable of normal rotation（Core
Pipe is connected with outer segment, and when rotating core pipe, diaphragm plate, outer segment is also required for certain locomotivity）, outer segment
The pipe of the type such as sebific duct, oil cylinder petroleum pipeline can be selected.
It is provided with the sealing depression bar 7 of vertical arrangement, sealing depression bar passes through all diaphragm plates and seals depression bar in described rotation pipe
All threaded between all diaphragm plates, sealing depression bar is provided with some pressure strips 71 for compressing hole sealing plate, pressure strip
Correspond with hole sealing plate, pressure strip is in the top of corresponding hole sealing plate.As it was previously stated, filling it up with liquid, Ran Houfeng in rotation pipe
Orifice plate seals after connecting lumen pore, separates between each quantitative chamber.But, in order to allow hole sealing plate will the thorough company's of sealing lumen pore, hole sealing plate
Mutual extruding and one piece of diaphragm plate of lower contact between is necessarily larger.After being used for multiple times, easily lead to because wearing and tearing larger
Sealing is not enough.And in this programme, hole sealing plate seals after connecting lumen pore, depression bar can be sealed with turn, drive pressure strip to move down and press
Tightly corresponding hole sealing plate, improves sealing further, ensures the cut-off effect between each quantitative chamber.And have sealing depression bar, pressure
After tight plate, during fabrication, too tight without pressure between hole sealing plate and diaphragm plate（Sealing can be protected by pushing of pressure strip
Barrier）, such that it is able to effectively reducing abrasion, increasing the service life.It is noted that after having sealing depression bar, pressure strip, turning allowing
Dynamic pipe is motionless and turn core pipe before, pressure strip should be allowed to be not located at compressing the state of corresponding hole sealing plate, " turning motionless " otherwise occurs
Situation.
Claims (7)
1. a kind of multiple field cell culture device, is characterized in that, including the culture vessel of base and some upper end open, each culture
Vessel stack gradually from the bottom to top, a culture vessel contact base of bottom, and described base is provided with fixing vertical rod, culture
Vessel lateral wall is provided with the retaining collar being adapted to fixing vertical rod, and retaining collar is set in fixing vertical rod, culture vessel bag
Include ware body, the column being arranged on bottom in ware body and the liquid injection pipe being arranged on ware body sidewall and being in tilted layout, column is upper and lower
The equal opening in two ends, through all columns, described rotation pipe and column can relatively rotate a rotation pipe vertically arranged, and rotate
In sealing contact between pipe lateral wall and column medial wall, rotate pipe lateral wall and be provided with and run through the discharge opeing rotating pipe upper/lower terminal
Groove, in a culture vessel：Column is provided with the equal liquid hole connecting with portion in ware body, is provided with cell filtering net in equal liquid hole,
Liquid injection pipe lower end is connected with portion in ware body.
2. a kind of multiple field cell culture device according to claim 1, is characterized in that, if described base include matrix and
The dry support column being arranged on below matrix, matrix is provided with the bottom through hole running through the upper and lower surface of matrix, rotates pipe lower end and stretches into bottom
In through hole, it is provided with the opening up fluid cylinder that connects below the through hole of bottom, below matrix, is provided with the bottom bracing being connected with least one support column
Plate, bottom bracing plate is provided with and connects the cylinder groove that fluid cylinder bottom is adapted to, and connects fluid cylinder bottom and is in a groove.
3. a kind of multiple field cell culture device according to claim 1, is characterized in that, is provided with core pipe in described rotation pipe
And some horizontally disposed diaphragm plates, core pipe lower end is stretched in the through hole of bottom, and core pipe is arranged with support ring, and support ring is higher than core pipe
Bottom and support ring is in the through hole of bottom, is provided with support base in the through hole of bottom, support base top be provided with seat slot and with rotate pipe lower end
The annular groove of adaptation, support ring bottom contacts seat slot bottom land, rotates pipe lower end and stretches in annular groove and rotate bottom of the tube contact annular groove groove
Bottom, is rotated pipe and is rotatably assorted with support base, and core pipe passes through support base and core pipe is rotatably assorted with support base, and diaphragm plate is set in core
Guan Shang, diaphragm plate cross section is in annular, and rotation pipe, core pipe and each diaphragm plate are all coaxial, and core pipe can relatively rotate with diaphragm plate,
In sealing contact between core pipe and diaphragm plate, sealing between diaphragm plate and rotation pipe is fixed；
Diaphragm plate is provided with even lumen pore, and core pipe is provided with some hole sealing plates for the company's of sealing lumen pore, hole sealing plate and diaphragm plate one
One is corresponding, in corresponding hole sealing plate with diaphragm plate：Hole sealing plate is in above diaphragm plate, and hole sealing plate bottom surface contacts tabula top of the plate
Face；
Rotate in pipe and form quantitative chamber between adjacent two pieces of diaphragm plates, if rotating pipe to be provided with main to fluid apertures, quantitative chamber and pipe
Correspond to fluid apertures, quantitative chamber is corresponded with culture vessel, in corresponding quantitative chamber with culture vessel：Column is provided with
Fluid apertures given by cylinder, and quantitative chamber is passed through corresponding pipe and is communicated to cylinder to fluid apertures to fluid apertures, pipe to fluid apertures with cylinder to fluid apertures all in cylinder,
And the pipe that interconnects is coaxial to fluid apertures with cylinder to fluid apertures, to fluid apertures axis horizontal, quantitative chamber lowest part is higher than cylinder to fluid apertures to cylinder
Lowest part, cylinder is higher than equal liquid hole highest point to fluid apertures lowest part.
4. a kind of multiple field cell culture device according to claim 3, is characterized in that, in corresponding quantitative chamber and culture
In vessel：Quantitative intracavity is provided with tune amount liquid capsule, and tune amount liquid capsule is provided with for the feed pipe to tune amount liquid capsule feed flow, feed pipe one
End is connected with tune amount liquid capsule, and feed pipe is made up of the hard tube section being in quantitative intracavity and the outer segment being in outside quantitative chamber；
Base be provided with some for the confession fluid cylinder to feed pipe feed flow, include cylinder body and cylinder body slipper seal cooperation for fluid cylinder
Feed flow piston and be connected and be in the piston rod above feed flow piston with feed flow piston, the slidably direction of feed flow piston is upper
Lower direction, supplies in fluid cylinder at one：Space below feed flow piston in cylinder body is sap cavity, the space above feed flow piston in cylinder body
For adjusting chamber；
Sap cavity and feed pipe correspond, and the feed pipe other end connect with corresponding sap cavity, feed pipe through rotation tube side wall and
Rotate pipe lower end, sealing between feed pipe and rotation tube side wall is fixed.
5. a kind of multiple field cell culture device according to claim 4, is characterized in that, described base is provided with and base
Fixing adjustment seat, is provided with horizontally disposed adjustable plate below adjustment seat, piston rod passes through adjustable plate, and piston rod is provided with pressure
Block and lower drawing block, adjustment seat is provided with the vertical homophony pole arranged and pass through adjustment seat, and homophony pole is with adjustment seat screw thread even
Connect, homophony pole is provided with upper holder block and lower drawing block, the contact of upper holder block bottom surface adjusts plate top surface, and lower drawing block top surface contacts adjustable plate
Bottom surface, main regulation masthead portion is provided with the main regulation knob fixing with homophony pole.
6. a kind of multiple field cell culture device according to claim 5, is characterized in that, described homophony pole is provided with place
Instruction disk above adjustment seat, instruction disk is coaxial with homophony pole, and adjustment seat is provided with the indicating arm of groove with a scale, indicating arm
Vertically arrangement and indicating arm contact instruction Pan Cebi.
7. a kind of multiple field cell culture device according to claim 3 or 4 or 5, is characterized in that, sets in described rotation pipe
There is the sealing depression bar of vertically arrangement, all screw thread connects sealing depression bar through all diaphragm plates and sealing depression bar and all diaphragm plates
Connect, sealing depression bar is provided with some pressure strips for compressing hole sealing plate, pressure strip is corresponded with hole sealing plate, and pressure strip is in
The top of corresponding hole sealing plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620929362.3U CN205933844U (en) | 2016-08-22 | 2016-08-22 | Multilayer formula cell breeding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620929362.3U CN205933844U (en) | 2016-08-22 | 2016-08-22 | Multilayer formula cell breeding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205933844U true CN205933844U (en) | 2017-02-08 |
Family
ID=57952101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620929362.3U Active CN205933844U (en) | 2016-08-22 | 2016-08-22 | Multilayer formula cell breeding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205933844U (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843A (en) * | 2016-08-22 | 2016-12-07 | 王铁苗 | A kind of multisample culture apparatus |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
-
2016
- 2016-08-22 CN CN201620929362.3U patent/CN205933844U/en active Active
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047703A (en) * | 2016-08-22 | 2016-10-26 | 王铁苗 | Test sample culture apparatus |
| CN106190843A (en) * | 2016-08-22 | 2016-12-07 | 王铁苗 | A kind of multisample culture apparatus |
| CN106222087A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of sample culture carrier |
| CN106222090A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test assistant carrier |
| CN106222089A (en) * | 2016-08-22 | 2016-12-14 | 王铁苗 | A kind of test auxiliary culture apparatus |
| CN106244457A (en) * | 2016-08-22 | 2016-12-21 | 王铁苗 | Culture carrier structure is used in a kind of test |
| CN106282017A (en) * | 2016-08-22 | 2017-01-04 | 王铁苗 | A kind of integrated form sample culture apparatus |
| CN106467893A (en) * | 2016-08-22 | 2017-03-01 | 王铁苗 | A kind of experiment auxiliary device |
| CN106190843B (en) * | 2016-08-22 | 2018-06-15 | 刘力伟 | A kind of multisample culture apparatus |
| CN106467893B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment auxiliary device |
| CN106222090B (en) * | 2016-08-22 | 2018-07-24 | 王铁苗 | A kind of experiment assistant carrier |
| CN106244457B (en) * | 2016-08-22 | 2018-08-03 | 日照安维信生物科技有限公司 | A kind of experiment culture carrier structure |
| CN106047703B (en) * | 2016-08-22 | 2018-08-10 | 青岛爱博生物科技有限公司 | A kind of test sample culture apparatus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205933844U (en) | Multilayer formula cell breeding device | |
| CN205974549U (en) | Cell culture device | |
| CN205933846U (en) | Cell in vitro culture ware | |
| CN205907270U (en) | Cultivate carrier structure | |
| CN106190839A (en) | A kind of cell culture system that can be used for Comparability test | |
| CN106244453B (en) | A kind of cell culture device for cultivating, measuring and analyzing | |
| CN106222087B (en) | A kind of sample culture carrier | |
| CN106244457A (en) | Culture carrier structure is used in a kind of test | |
| CN106467893A (en) | A kind of experiment auxiliary device | |
| CN106119110A (en) | A kind of culture carrier structure | |
| CN106222090B (en) | A kind of experiment assistant carrier | |
| CN106222089B (en) | A kind of experiment auxiliary culture apparatus | |
| CN106190843A (en) | A kind of multisample culture apparatus | |
| CN106282017B (en) | A kind of integrated form sample culture apparatus | |
| CN204907394U (en) | Movable drips irrigation device | |
| CN208684977U (en) | A kind of multi-layer cellular culture bottle | |
| CN106047703B (en) | A kind of test sample culture apparatus | |
| CN208869585U (en) | A kind of fruit wine preparation fruit juice activated yeast tank | |
| CN209173913U (en) | Can quantitative liquid-filling reactor tank | |
| CN105039150A (en) | Airlift fermentor with sieve plates | |
| CN206525296U (en) | A kind of cultivation apparatus without soil | |
| CN205933793U (en) | Culture medium ration partial shipment ware | |
| CN218353519U (en) | Water supplementing device for seedling raising of pakchoi | |
| CN215648626U (en) | Quantitative watering device | |
| CN208940482U (en) | A kind of seedling incubator convenient for operation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 311200, Zhejiang province Hangzhou city Xiaoshan District Xiaoshan science and technology city B block 3 floor Rong Ze biology Patentee after: Hangzhou Rongze Biotechnology Group Co.,Ltd. Address before: 311200, Zhejiang province Hangzhou city Xiaoshan District Xiaoshan science and technology city B block 3 floor Rong Ze biology Patentee before: HANGZHOU RONGZE BIOTECHNOLOGY Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |