CN211921518U - Liquid exchanger for cell culture - Google Patents
Liquid exchanger for cell culture Download PDFInfo
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- CN211921518U CN211921518U CN202020209343.XU CN202020209343U CN211921518U CN 211921518 U CN211921518 U CN 211921518U CN 202020209343 U CN202020209343 U CN 202020209343U CN 211921518 U CN211921518 U CN 211921518U
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- cell culture
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Abstract
The utility model discloses a liquid exchanger for cell culture, when absorbing culture medium, the second valve is opened, the first valve is closed, and the adsorption equipment acts, so that the liquid outlet pipe absorbs the culture medium in the culture medium accommodating layer, the culture medium in the cell culture layer enters the culture medium accommodating layer through the semipermeable membrane area, flows through the liquid adding channel, enters the liquid outlet pipe, and is finally absorbed through the adsorption equipment; rinsing cells, opening the first valve by closing the second valve, enabling the extraction equipment to act, extracting buffer solution to enter the liquid adding channel, slowly permeating the buffer solution into the cell culture layer through the semipermeable membrane area, closing the first valve after covering, connecting the connecting pipe with adsorption equipment, opening the second valve, and absorbing the buffer solution; prevent to cause the pollution to the cell at the liquid changing in-process, avoid local high hydraulic pressure to cause the impact to the cell to this carries out better protection to the cell.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a liquid changing device for cell culture.
Background
Cell culture is a widely used technique in many biological laboratories, biotechnology or medical companies. Cells can be largely divided into suspension cells, which are mainly suspended in the culture medium, and adherent cells, which are more prone to adhere to the surface of the culture vessel during growth, depending on their growth state. In order to keep a good nutrition state of cells in a culture process, the culture medium needs to be replaced at regular time, the cells are rinsed by buffer solution, and then the new culture medium is replaced.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a liquid changer for cell culture aims at solving the cell among the prior art and changes the in-process of culture medium and current liquid changer can't play better guard action to the cell, easily causes the technical problem of influence to the growth state of cell.
In order to achieve the above purpose, the utility model discloses a liquid exchanger for cell culture, which comprises an incubator, a three-way pipe, a connecting pipe, a liquid outlet pipe, a first valve and a second valve, wherein the incubator is of a double-layer structure, the incubator comprises a cell culture layer and a culture medium accommodating layer, the culture medium accommodating layer is sleeved outside the cell culture layer, a liquid feeding channel is formed between the culture medium accommodating layer and the cell culture layer, a semi-permeable membrane area is arranged at the bottom of the cell culture layer, a first port of the three-way pipe is communicated with the liquid feeding channel, a second port of the three-way pipe is communicated with one end of the connecting pipe, a third port of the three-way pipe is communicated with one end of the liquid outlet pipe, the other end of the liquid outlet pipe is communicated with the liquid feeding channel, the first valve is arranged at the joint of the first port of the three-way pipe and the liquid feeding channel, the second valve is arranged at the joint of the liquid outlet pipe and the liquid feeding channel.
Wherein, a liquid exchanger for cell culture still includes water blocking spare, water blocking spare sets up the inside of liquid feeding passageway, just water blocking spare's quantity is a plurality of, adjacent two water blocking spare is followed the circulation direction of liquid feeding passageway is crisscross setting in proper order.
The cross section of each water blocking piece is arranged in a V-shaped structure, and the open end of each water blocking piece faces to the outside of the cell culture layer and the inside of the culture medium accommodating layer.
The liquid changer for cell culture further comprises a buffer plate, the buffer plate is arranged inside the liquid adding channel and located below the water retaining piece, the buffer plate is close to the joint of the liquid outlet pipe and the liquid adding channel, and an overflow channel is arranged on the buffer plate.
Wherein the width of the overflow channel is 0.5 mm-1.0 mm.
The liquid exchanger for cell culture further comprises a buffer piece, wherein the buffer piece is arranged inside the liquid feeding channel and is positioned between the buffer plate and the joint of the liquid outlet pipe and the liquid feeding channel.
Wherein, the cross section of bolster personally submits the domes setting.
Wherein, a liquid change ware for cell culture still includes the pellicle, the pellicle attaches to the pellicle is regional, just the tectorial membrane aperture of pellicle is 0.1 mu m ~ 0.9 mu m.
The beneficial effects of the utility model are embodied in: when the connecting pipe is connected with an adsorption device during culture medium suction, the second valve is opened, the first valve is closed, and the adsorption device acts to enable the liquid outlet pipe to suck the culture medium in the culture medium containing layer of the lower layer, so that the culture medium in the cell culture layer of the upper layer enters the culture medium containing layer of the lower layer through the semipermeable membrane area, flows through the liquid feeding channel, enters the liquid outlet pipe, and is finally sucked by the adsorption device through the connecting pipe; when cells are rinsed, the connecting pipe is connected with an extraction device, then the second valve is closed, the first valve is opened, buffer solution is extracted through the connecting pipe by the action of the extraction device, enters the liquid adding channel and is added into the culture medium containing layer at the lower layer, the buffer solution can slowly permeate into the cell culture layer at the upper layer through the semipermeable membrane area, after the upper layer is covered, the first valve is closed, the connecting pipe is connected with an adsorption device, the second valve is opened, and the buffer solution is sucked away through the liquid outlet pipe; the liquid in-process is being traded, trades the liquid instrument and not direct contact cell surface, can prevent to cause the pollution to the cell, can also reduce because of trading the liquid and open the pollution that the incubator brought many times for the cell, also avoids trading the liquid in-process simultaneously, and local high hydraulic pressure causes the impact to the cell to this carries out better protection to the cell.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic diagram of the liquid exchanger for cell culture according to the present invention.
FIG. 2 is a sectional view showing the structure of the liquid exchanger for cell culture according to the present invention.
100-liquid exchanger for cell culture, 10-incubator, 101-cell culture layer, 102-culture medium accommodating layer, 103-semi-permeable membrane region, 11-three-way pipe, 12-connecting pipe, 13-liquid outlet pipe, 14-first valve, 15-second valve, 16-water retaining piece, 17-buffer plate, 171-overflow channel, 18-buffer piece and 19-semi-permeable membrane.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. In addition, in the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Referring to fig. 1 and 2, the present invention provides a liquid exchanger 100 for cell culture, including an incubator 10, a three-way pipe 111, a connecting pipe 12, a liquid outlet pipe 13, a first valve 14 and a second valve 15, wherein the incubator 10 is a double-layer structure, the incubator 10 includes a cell culture layer 101 and a culture medium accommodating layer 102, the culture medium accommodating layer 102 is sleeved outside the cell culture layer 101, a liquid feeding channel is formed between the culture medium accommodating layer 102 and the cell culture layer 101, a semi-permeable membrane 19 region 103 is arranged at the bottom of the cell culture layer 101, a first port of the three-way pipe 111 is communicated with the liquid feeding channel, a second port of the three-way pipe 111 is communicated with one end of the connecting pipe 12, a third port of the three-way pipe 111 is communicated with one end of the liquid outlet pipe 13, and the other end of the liquid outlet pipe 13 is communicated with the liquid feeding channel, the first valve 14 is arranged at the joint of the first port of the three-way pipe 111 and the liquid charging channel, and the second valve 15 is arranged at the joint of the liquid outlet pipe 13 and the liquid charging channel.
In this embodiment, the incubator 10 is used for culturing suspension cells or adherent cells, and the suspension cells or the adherent cells are located in the cell culture layer 101, the second port of the three-way pipe 111 is communicated with one end of the connecting pipe 12, and the other end of the connecting pipe 12 is communicated with an adsorption device or an extraction device;
when the culture medium is sucked, by connecting the connecting pipe 12 with an adsorption device and then opening the second valve 15, closing the first valve 14, and making the liquid outlet pipe 13 suck the culture medium in the culture medium accommodating layer 102 of the lower layer by the action of the adsorption device, the culture medium in the cell culture layer 101 of the upper layer will enter the culture medium accommodating layer 102 of the lower layer through the region 103 of the semi-permeable membrane 19, flow through the liquid feeding channel, enter the liquid outlet pipe 13, and finally enter the connecting pipe 12 through the three-way pipe 111 to be sucked by the adsorption device; when cells are rinsed, the connecting pipe 12 is connected with an extraction device, then the second valve 15 is closed, the first valve 14 is opened, external buffer solution is extracted through the connecting pipe 12 by the action of the extraction device, enters the liquid adding channel through the three-way pipe 111 and is added into the culture medium accommodating layer 102 at the lower layer, the buffer solution can slowly permeate into the cell culture layer 101 at the upper layer through the area 103 of the semipermeable membrane 19 after covering the upper layer, then the first valve 14 is closed, the connecting pipe 12 is connected with an adsorption device, the second valve 15 is opened, the buffer solution is sucked into the liquid outlet pipe 13 from the liquid adding channel by the action of the adsorption device, and finally enters the connecting pipe 12 through the three-way pipe 111 and is sucked by the adsorption device;
then the second valve 15 is closed, the first valve 14 is opened, an extraction device is installed on the connecting pipe 12, the extraction device extracts new culture medium into the liquid feeding channel and then adds the new culture medium into the culture medium accommodating layer 102 at the lower layer, the new culture medium can slowly permeate into the cell culture layer 101 at the upper layer through the area 103 of the semi-permeable membrane 19 until the upper layer is covered, and then the first valve 14 is closed, thereby completing the liquid change of the cell culture. In the liquid changing process, a liquid changing instrument does not directly contact the surface of a cell, the cell can be prevented from being polluted, the pollution caused by opening the incubator 10 for the cell for many times due to liquid changing can be reduced, meanwhile, the liquid adding and liquid changing process is also avoided, the cell is easy to be washed up, the density uniformity during cell inoculation is influenced, in addition, the liquid changing process can be avoided, local hydraulic pressure causes impact on the cell, the cell is prevented from being injured, and therefore a better protection effect is achieved on the cell.
Further, a liquid changing device 100 for cell culture still includes water blocking piece 16, water blocking piece 16 sets up the inside of liquid feeding passageway, just water blocking piece 16's quantity is a plurality of, adjacent two water blocking piece 16 is followed liquid feeding passageway's circulation direction is crisscross setting in proper order.
In this embodiment, the plurality of water baffles 16 are sequentially arranged in a staggered manner along the flowing direction of the liquid feeding channel, so that a buffer solution or a new culture medium entering the liquid feeding channel through the first port of the three-way pipe 111 can slowly enter the culture medium accommodating layer 102 at a constant speed and slowly permeate into the cell culture layer 101, thereby avoiding that the buffer solution or the new culture medium is too large in impact force to cause damage to cells, being more beneficial to protecting the cells and avoiding influencing the growth state of the cells.
Further, the cross section of each water blocking member 16 is arranged in a V-shaped structure, and the open end of each water blocking member 16 faces the outside of the cell culture layer 101 and the inside of the medium accommodating layer 102.
In this embodiment, the water blocking member 16 is arranged in a V-shaped structure, and can play a better role in buffering a buffer solution or a new culture medium, so that the buffer solution or the new culture medium can enter the culture medium accommodating layer 102 and the cell culture layer 101 at a uniform speed.
Further, the liquid changer 100 for cell culture further comprises a buffer plate 17, the buffer plate 17 is disposed inside the liquid feeding channel and below the water blocking member 16, the buffer plate 17 is close to the connection between the liquid outlet pipe 13 and the liquid feeding channel, and the buffer plate 17 is provided with an overflow channel 171. The width of the overflow passage 171 is 0.5 mm to 1.0 mm.
In this embodiment, the width of the overflow channel 171 is preferably 0.8mm, and the arrangement of the buffer plate 17 and the overflow channel 171 can further play a role in buffering and stabilizing the buffer solution or new culture medium flowing into the culture medium accommodating layer 102, reduce the impact force when the buffer solution or new culture medium in the culture medium accommodating layer 102 is added, avoid damage to the cells, be more beneficial to protecting the cells, and avoid influence on the growth state of the cells, meanwhile, the suction force of the adsorption equipment and the liquid outlet pipe 13 when adsorbing the culture medium to be replaced or the buffer solution after rinsing can be dispersed, so that the culture medium to be replaced or the buffer solution after rinsing can slowly flow into the liquid outlet pipe 13 at a constant speed, then sucking out the culture medium through the adsorption device, thereby preventing the cell from being damaged due to overlarge suction force when the culture medium needing to be replaced or the buffer solution after rinsing is sucked.
Further, the liquid exchanger 100 for cell culture further comprises a buffer member 18, wherein the buffer member 18 is arranged inside the liquid feeding channel and is positioned between the buffer plate 17 and the connection part of the liquid outlet pipe 13 and the liquid feeding channel. The bumper 18 is provided with an arched structure in cross section.
In this embodiment, the buffer component 18 can enable the buffer solution or the new culture medium entering the liquid feeding channel through the first port of the three-way pipe 111 to enter the culture medium accommodating layer 102 more uniformly and slowly permeate into the cell culture layer 101, so that the buffer solution or the new culture medium is prevented from being damaged by excessive impact force, cells are protected more easily, and the growth state of the cells is prevented from being affected. And the cross section of the buffer member 18 is in an arch structure, so that the impact force generated by the buffer solution or new culture medium entering the liquid feeding channel through the first port of the three-way pipe 111 can be further reduced.
Further, the liquid changer 100 for cell culture further comprises a semipermeable membrane 19, wherein the semipermeable membrane 19 is attached to the region 103 of the semipermeable membrane 19, and the pore diameter of the membrane of the semipermeable membrane 19 is 0.1 to 0.9 μm.
In the present embodiment, the pore diameter of the membrane 19 is preferably 0.5 μm, so that it is possible to ensure that a liquid can pass through the membrane 19 but a cell cannot pass through it. Thereby realizing the liquid change operation of the cells.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (8)
1. A liquid changer for cell culture is characterized in that,
comprises an incubator, a three-way pipe, a connecting pipe, a liquid outlet pipe, a first valve and a second valve, wherein the incubator is of a double-layer structure, the culture box comprises a cell culture layer and a culture medium accommodating layer, the culture medium accommodating layer is sleeved outside the cell culture layer, a priming channel is formed between the culture medium accommodating layer and the cell culture layer, the bottom of the cell culture layer is provided with a semi-permeable membrane area, the first port of the three-way pipe is communicated with the liquid feeding channel, the second port of the three-way pipe is communicated with one end of the connecting pipe, the third port of the three-way pipe is communicated with one end of the liquid outlet pipe, the other end of the liquid outlet pipe is communicated with the liquid feeding channel, the first valve is arranged at the joint of the first port of the three-way pipe and the liquid feeding channel, and the second valve is arranged at the joint of the liquid outlet pipe and the liquid feeding channel.
2. The fluid changer for cell culture according to claim 1,
the liquid changer for cell culture further comprises a water blocking piece, wherein the water blocking piece is arranged inside the liquid feeding channel, the number of the water blocking pieces is multiple and adjacent to the water blocking pieces, and the water blocking pieces are arranged along the flowing direction of the liquid feeding channel in a staggered mode in sequence.
3. The fluid changer for cell culture according to claim 2,
every the cross section of water blocking piece is the setting of V type structure, and every the open end of water blocking piece is towards respectively the outside on cell culture layer, and the inside setting on culture medium holding layer.
4. The fluid changer for cell culture according to claim 3,
the liquid changer for cell culture further comprises a buffer plate, the buffer plate is arranged inside the liquid adding channel and located below the water retaining piece, the buffer plate is close to the joint of the liquid outlet pipe and the liquid adding channel, and an overflow channel is arranged on the buffer plate.
5. The fluid changer for cell culture according to claim 4,
the width of the overflow channel is 0.5 mm-1.0 mm.
6. The fluid changer for cell culture according to claim 5,
the liquid exchanger for cell culture further comprises a buffer piece, wherein the buffer piece is arranged inside the liquid feeding channel and is positioned between the buffer plate and the junction of the liquid outlet pipe and the liquid feeding channel.
7. The fluid changer for cell culture according to claim 6,
the cross section of the buffer piece is in an arch structure.
8. The fluid changer for cell culture according to any one of claims 1 to 7,
the liquid exchanger for cell culture further comprises a semipermeable membrane, wherein the semipermeable membrane is attached to the region of the semipermeable membrane, and the aperture of the membrane of the semipermeable membrane is 0.1-0.9 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020209343.XU CN211921518U (en) | 2020-02-20 | 2020-02-20 | Liquid exchanger for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020209343.XU CN211921518U (en) | 2020-02-20 | 2020-02-20 | Liquid exchanger for cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN211921518U true CN211921518U (en) | 2020-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020209343.XU Expired - Fee Related CN211921518U (en) | 2020-02-20 | 2020-02-20 | Liquid exchanger for cell culture |
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| CN (1) | CN211921518U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
-
2020
- 2020-02-20 CN CN202020209343.XU patent/CN211921518U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
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Granted publication date: 20201113 |