CN217499268U

CN217499268U - Be used for cell culture bag to divide bag, fluid infusion device

Info

Publication number: CN217499268U
Application number: CN202221538832.5U
Authority: CN
Inventors: 黄国军; 陶其强; 王娅莎; 范金富
Original assignee: Hainan Qiyan Stem Cell Anti Aging Hospital Co ltd
Current assignee: Hainan Qiyan Stem Cell Anti Aging Hospital Co ltd
Priority date: 2022-06-20
Filing date: 2022-06-20
Publication date: 2022-09-27
Anticipated expiration: 2032-06-20

Abstract

The utility model discloses a bag separating and liquid supplementing device for cell culture bags, which relates to the technical field of biological experiment appliances and comprises a liquid storage tank, wherein the bottom end of the liquid storage tank is connected with a supporting chassis, the top end of the liquid storage tank is connected with a first culture bag, the outer surface of the bottom end of the liquid storage tank is connected with a second culture bag, the device can clearly observe the volume of the culture liquid injected into the first culture bag in the tank body through the matching of the first culture bag and the second culture bag with scale marks positioned on the outer surface of the tank body, thereby being capable of accurately controlling the volume of liquid separation and liquid supplementation in the second culture bag, ensuring that the two culture bags can obtain the culture liquid with equal volume, improving the accuracy of liquid separation, ensuring that the culture medium in the tank body can automatically flow into the second culture bag along with the gravity, and keeping the joint of the second liquid separation pipe and the second culture bag away from the table board of a biological safety cabinet, reduce external pollution's risk, improve work efficiency.

Description

Be used for cell culture bag to divide bag, fluid infusion device

Technical Field

The utility model relates to a biological assay utensil technical field specifically is a be used for cell culture bag to divide bag, fluid infusion device.

Background

Nowadays, cell culture bags are commonly used in the field of cell biology experiments, especially when the cells need to be cultured in a large amount in a sterile environment.

In the process of culturing cells, in order to rapidly amplify and culture a large quantity of high-quality cells, an operator needs to perform liquid supplementing operation on the cells cultured in the cell culture bag for many times, and corresponding nutrient substances are continuously supplemented; at present, a common operation method in the operation of cell amplification culture fluid infusion in a biological laboratory is to use a 50ml syringe bracket to support a 50ml syringe tube body, and then insert a 50ml syringe liquid outlet into a culture bag tube mouth to play a role in communication, so that the operation has the risk of separation and falling, and the separation and falling can cause cell culture failure; meanwhile, the volume of the fluid infusion is difficult to accurately grasp by a 50ml syringe, so that the total volume of the fluid infusion is not uniform, the adjustment of the cell culture density is influenced, and the probability that the number of cells cannot be greatly expanded is greatly increased; meanwhile, because the capacity of the 50ml syringe is small, the operator needs to pour the culture medium for multiple times to replenish the liquid, and the frequent pouring operation can not only make the arm of the operator limp, but also increase the risk of pollution.

Based on this, the utility model designs a be used for cell culture bag to divide bag, fluid infusion device to solve the above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

The utility model provides a not enough to prior art, the utility model provides a be used for cell culture bag to divide bag, fluid infusion device has solved artifical manual fluid infusion, has divided the numerous and diverse problem that the pollution risk is big of liquid operation.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: a bag separating and fluid supplementing device for a cell culture bag comprises a fluid reservoir, wherein the bottom end of the fluid reservoir is connected with a supporting chassis, the top end of the fluid reservoir is connected with a first culture bag, and the outer surface of the bottom end of the fluid reservoir is connected with a second culture bag;

the liquid storage pot includes a jar body, the top of the jar body is connected with the bottle lid, the top of bottle lid is provided with first branch liquid pipe, the surface of jar body bottom is provided with the second and divides liquid pipe.

As a further technical scheme of the utility model, the one end of first cultivation bag is connected with first connecting pipe, the one end of first connecting pipe is connected with first liquid distribution pipe, the surface of first connecting pipe is provided with first ooff valve, first cultivation bag is connected with the jar body through first connecting pipe and first liquid distribution pipe.

As a further technical scheme of the utility model, the one end of bag is cultivateed to the second is connected with the second connecting pipe, the one end of second connecting pipe is connected with the second and divides the liquid pipe, the surface of second connecting pipe is provided with the second ooff valve, the bag is cultivateed to the second is connected with the jar body through second connecting pipe and second branch liquid pipe.

As a further technical scheme of the utility model, jar body and bottle lid are the glass material, the surface of jar body is provided with a plurality of scale marks, connecting thread has been seted up to the surface on jar body top, the inside wall and the connecting thread correspondence of bottle lid are seted up there is the internal thread, the internal thread and the connecting thread looks adaptation of bottle lid.

As a further technical scheme of the utility model, the bottom of first branch liquid pipe is linked together with the inside of the jar body, the one end of second branch liquid pipe is linked together with the inside of the jar body, the second divides the liquid pipe to be the inclination installation, just the interior bottom surface of the jar body is the inclined plane, the connector looks parallel and level that is located jar internal portion of the interior bottom surface of the jar body and second branch liquid pipe.

As a further technical scheme of the utility model, the internal diameter size of first minute liquid pipe and the internal diameter size looks adaptation of first connecting pipe, the internal diameter size of second minute liquid pipe and the internal diameter size looks adaptation of second connecting pipe.

Advantageous effects

The utility model provides a bag separating and liquid supplementing device for cell culture bags. Compared with the prior art, the method has the following beneficial effects:

1. the utility model provides a be used for cell culture bag to divide the bag, the fluid infusion device, first culture bag is through first connecting pipe and first ooff valve and first minute liquid pipe and jar body coupling, and the second culture bag divides the liquid pipe through second connecting pipe and second ooff valve and second and is connected with jar body, the scale mark that is located jar external surface can clearly observe how much of the volume of the culture solution that jar internal portion's first culture bag poured into, thereby can accurate control divide the culture solution of the interior target volume of second culture bag, guarantee that two culture bags can obtain the culture solution of impartial volume, improve the accurate nature of dividing the liquid.

2. The utility model provides a mesa that is used for cell culture bag to divide bag, fluid infusion device installs in external surface of the tank body, the second divides the liquid pipe through the slope, and the second divides the liquid pipe to be located the internal portion of jar inclined plane looks parallel and level of the internal portion bottom surface of connector jar for the internal culture medium of jar can be along with gravity flows in the second culture bag automatically, and the second divides the liquid pipe to keep away from biological safety cabinet with the junction of second culture bag, reduces the risk of external pollution, improves work efficiency.

3. The utility model provides a be used for cell culture bag to divide bag, fluid infusion device, can make things convenient for opening and adding fresh culture solution of bottle lid between the connecting thread on stock solution jar top and the bottle lid, and the second culture bag divides the liquid pipe to be connected with jar body through second connecting pipe and second ooff valve and second, the scale mark that is located jar external surface can clearly observe how much of the volume that jar internal fresh culture medium of portion poured into to can accurate control replenish the culture solution to the interior target volume of second culture bag, improve the accurate nature of fluid infusion.

Drawings

FIG. 1 is a schematic structural view of a bag separating and replenishing device for cell culture bags;

FIG. 2 is an exploded view of a fluid reservoir for a bag-separating and fluid-replenishing device of a cell culture bag;

FIG. 3 is a left side view of a fluid reservoir for a bag-splitting, fluid infusion device for cell culture bags;

FIG. 4 is a sectional view A-A of FIG. 3, showing a fluid replacement device for a cell culture bag.

In the figure: 1. a liquid storage tank; 11. a tank body; 12. a bottle cap; 13. connecting threads; 14. a first liquid dividing pipe; 15. a second liquid dividing pipe; 16. scale lines; 2. a support chassis; 3. a first culture bag; 31. a first connecting pipe; 32. a first on-off valve; 4. a second culture bag; 41. a second connecting pipe; 42. and a second on-off valve.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a solution for separating and replenishing cell culture bags: the utility model provides a be used for cell culture bag to divide bag, fluid infusion device, includes liquid storage pot 1, and the bottom of liquid storage pot 1 is connected with supports chassis 2, and the top of liquid storage pot 1 is connected with first culture bag 3, and the surface of 1 bottom of liquid storage pot is connected with second culture bag 4, and liquid storage pot 1 includes jar body 11, and the top of jar body 11 is connected with bottle lid 12, and the top of bottle lid 12 is provided with first branch liquid pipe 14, and the surface of 11 bottoms of jar body is provided with second branch liquid pipe 15.

As shown in FIG. 1, a first connection tube 31 is connected to one end of a first culture bag 3, one end of the first connection tube 31 is connected to a first liquid distribution tube 14, a first switching valve 32 is provided on the outer surface of the first connection tube 31, the first culture bag 3 is connected to a tank 11 through the first connection tube 31 and the first liquid distribution tube 14, a second connection tube 41 is connected to one end of a second culture bag 4, one end of the second connection tube 41 is connected to a second liquid distribution tube 15, a second switching valve 42 is provided on the outer surface of the second connection tube 41, the second culture bag 4 is connected to the tank 11 through the second connection tube 41 and the second liquid distribution tube 15, the inner diameter of the first liquid distribution tube 14 is matched to the inner diameter of the first connection tube 31, the inner diameter of the second liquid distribution tube 15 is matched to the inner diameter of the second connection tube 41, it should be noted that the first culture bag 3 is controlled to distribute liquid to the inside of the tank 11 through the first connection tube 31 and the first switching valve 32 connected thereto, and the liquid is separated into the second culture bag 4 by the control of the tank 11, the second liquid separating tube 15, the second connecting tube 41 and the second on-off valve 42, so that the culture liquid in the first culture bag 3 and the culture liquid in the second culture bag 4 can be uniformly obtained.

As shown in fig. 2, the tank body 11 and the bottle cap 12 are made of glass, the outer surface of the tank body 11 is provided with a plurality of scale marks 16, the outer surface of the top end of the tank body 11 is provided with a connecting thread 13, the inner side wall of the bottle cap 12 is provided with an inner thread corresponding to the connecting thread 13, the inner thread of the bottle cap 12 is matched with the connecting thread 13, and it needs to be explained that the bottle cap 12 connected with the top end of the tank body 11 through the connecting thread 13 is convenient to disassemble and assemble, so that the fresh culture solution can be supplemented into the tank body 11, and the volume change of the liquid supplementation and the liquid separation of the tank body 11 can be clearly observed through the plurality of scale marks 16 on the outer surface of the tank body 11, so that the amount of the liquid supplementation and the liquid separation can be accurately controlled.

As shown in fig. 3-4, the bottom end of the first liquid distribution pipe 14 is connected to the inside of the tank 11, one end of the second liquid distribution pipe 15 is connected to the inside of the tank 11, the second liquid distribution pipe 15 is installed at an inclined angle, and the inner bottom surface of the tank 11 is an inclined surface, and the inner bottom surface of the tank 11 is flush with the connection port of the second liquid distribution pipe 15 located inside the tank 11, it should be noted that the cooperation between the second liquid distribution pipe 15 installed at an inclined angle and the inclined bottom surface inside the tank 11 can enable the culture medium in the tank 11 to naturally flow into the second culture bag 4 along with gravity, and the distance difference between the second liquid distribution pipe 15 and the inner bottom surface of the tank 11 can avoid the connection port of the second culture bag 4 from flatly placing on the table of the biosafety cabinet, thereby reducing the risk of contamination.

The utility model discloses a theory of operation: when the culture tank is used, the first connecting pipe 31 of the first culture bag 3 is connected to the connecting port of the first liquid dividing pipe 14, the first switch valve 32 is opened, so that the liquid in the first culture bag 3 flows into the tank body 11 along the first connecting pipe 31 and the first liquid dividing pipe 14, the amount of the culture solution divided in the first culture bag 3 can be observed through the scale mark 16 on the outer surface of the tank body 11, then, the first switch valve 32 is closed, and the second switch valve 42 is opened, so that the culture medium in the tank body 11 flows into the second culture bag 4 along the second liquid dividing pipe 15 and the second connecting pipe 41 along with gravity, so that the precise liquid division of the culture solution in the first culture bag 3 and the second culture bag 4 can be completed, and the two culture bags can obtain equal culture solution;

the bottle cap 12 at the top end of the tank 11 is unscrewed along the connecting thread 13, so that fresh culture solution can be injected into the tank 11, the amount of the injected culture solution can be clearly known through the scale marks 16 on the outer surface of the tank 11, and after sufficient fresh culture solution is added into the tank 11, the second switch valve 42 is opened, so that the culture medium in the tank 11 flows into the second culture bag 4 along the second liquid distribution pipe 15 and the second connecting pipe 41 along with gravity, and the solution supplement of the culture solution in the second culture bag 4 can be completed.

Claims

1. The bag separating and fluid supplementing device for the cell culture bags comprises a fluid storage tank (1) and is characterized in that the bottom end of the fluid storage tank (1) is connected with a supporting chassis (2), the top end of the fluid storage tank (1) is connected with a first culture bag (3), and the outer surface of the bottom end of the fluid storage tank (1) is connected with a second culture bag (4);

the liquid storage pot (1) comprises a pot body (11), the top end of the pot body (11) is connected with a bottle cap (12), the top end of the bottle cap (12) is provided with a first liquid distribution pipe (14), and the outer surface of the bottom end of the pot body (11) is provided with a second liquid distribution pipe (15).

2. The bag splitting and feeding device for the cell culture bag according to claim 1, wherein a first connection pipe (31) is connected to one end of the first culture bag (3), one end of the first connection pipe (31) is connected to the first liquid distribution pipe (14), a first switch valve (32) is arranged on the outer surface of the first connection pipe (31), and the first culture bag (3) is connected to the tank (11) through the first connection pipe (31) and the first liquid distribution pipe (14).

3. The bag dividing and feeding device for the cell culture bag according to claim 1, wherein a second connecting pipe (41) is connected to one end of the second culture bag (4), one end of the second connecting pipe (41) is connected to the second branch pipe (15), a second switch valve (42) is disposed on the outer surface of the second connecting pipe (41), and the second culture bag (4) is connected to the tank (11) through the second connecting pipe (41) and the second branch pipe (15).

4. The bag separating and fluid supplementing device for the cell culture bags according to claim 1, wherein the tank body (11) and the bottle cap (12) are made of glass, a plurality of scale marks (16) are arranged on the outer surface of the tank body (11), a connecting thread (13) is arranged on the outer surface of the top end of the tank body (11), an inner thread is correspondingly arranged on the inner side wall of the bottle cap (12) and the connecting thread (13), and the inner thread of the bottle cap (12) is matched with the connecting thread (13).

5. The bag separating and feeding device for the cell culture bag according to claim 1, wherein the bottom end of the first liquid separating pipe (14) is communicated with the inside of the tank body (11), one end of the second liquid separating pipe (15) is communicated with the inside of the tank body (11), the second liquid separating pipe (15) is installed at an inclined angle, the inner bottom surface of the tank body (11) is an inclined surface, and the inner bottom surface of the tank body (11) is flush with a connecting port of the second liquid separating pipe (15) inside the tank body (11).

6. The bag-splitting and fluid-supplementing device for a cell culture bag according to claim 3, wherein the inner diameter of the first branch pipe (14) is matched with the inner diameter of the first connecting pipe (31), and the inner diameter of the second branch pipe (15) is matched with the inner diameter of the second connecting pipe (41).