CN219342154U - Culture dish and culture device for solid culture medium - Google Patents
Culture dish and culture device for solid culture medium Download PDFInfo
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- CN219342154U CN219342154U CN202223399312.3U CN202223399312U CN219342154U CN 219342154 U CN219342154 U CN 219342154U CN 202223399312 U CN202223399312 U CN 202223399312U CN 219342154 U CN219342154 U CN 219342154U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The utility model discloses a culture dish for a solid culture medium and a culture device, comprising a cuboid culture dish body; at least two layers of culture layers are arranged in the culture dish body, each layer of culture layer is provided with a culture base, an overflow plate and overflow holes, each layer of culture layer divides the culture base and the overflow holes into left-right distribution structures through the overflow plate, and the overflow holes of the upper layer of culture layer are vertically arranged above the culture base of the lower layer of culture layer; the overflow plates are used for limiting the thickness of the culture medium on the corresponding culture bases; the top side of the culture dish body is provided with a breathing device; the culture dish body is provided with a liquid conveying port. The culture dish for the solid culture medium is characterized in that a plurality of culture layers are sequentially arranged in one culture dish body from top to bottom, so that the culture can be performed simultaneously, the occupied area is small, the culture quantity is large, and the required operators are greatly reduced.
Description
Technical Field
The utility model relates to a culture dish for a solid culture medium and a culture device.
Background
The culture dish is a commonly used laboratory vessel for microorganism or cell culture, and consists of a circular or rectangular bottom and a lid. The culture dish is mainly made of organic plastics and glass. Among them, a glass-made culture dish is generally used for plant material, microorganism culture, and adherent culture of animal cells. Most of plastic culture dishes are disposable, are suitable for laboratory inoculation, streaking and bacteria separation, and can also be used for culturing plant materials.
In the laboratory, when bacteria, microorganisms and the like are cultured by using a culture dish, a liquid culture medium is generally used for culturing, and the liquid culture medium culturing method mainly comprises liquid shaking culture or liquid submerged fermentation culture. However, for bacteria with special structures outside cell walls such as pili, a solid culture medium is generally adopted for culture so as to avoid the adoption of liquid shaking culture or liquid submerged fermentation culture, and the pili are not tightly connected and are easy to fall off too much, thereby influencing experiments. When the solid culture medium is used for culturing, the solid culture medium is melted and then the bottom of the plate is fully paved, and then bacteria are horizontally placed for static culture for a set time.
When the existing culture dish adopts a solid culture medium to culture special bacteria, the quantity of the cultured unit bacteria is small because the bottom area of the culture dish is small. If the culture dish is opened and then the inoculation or collection work is completed within a specified time during pilot test and pilot test, a large number of people are required to operate simultaneously, a plurality of clean operation tables are occupied simultaneously, the labor intensity is high, and the probability of cross contamination is increased.
Therefore, research and development of a culture dish for a solid culture medium can be used for mass inoculation, and the culture dish is small in occupied area and small in occupied operators, can greatly save resources for bacterial culture in a laboratory, and is very significant.
Disclosure of Invention
The utility model aims to provide a culture dish for a solid culture medium and a culture device.
In order to achieve the above purpose, the technical scheme of the utility model is as follows: a culture dish for a solid culture medium comprises a cuboid culture dish body; at least two layers of culture layers are arranged in the culture dish body, each layer of culture layer is provided with a culture base, an overflow plate and overflow holes, each layer of culture layer divides the culture base and the overflow holes into left-right distribution structures through the overflow plate, and the overflow holes of the upper layer of culture layers are vertically arranged above the culture bases of the lower layer of culture layers; the overflow plates are used for limiting the thickness of the culture medium on the corresponding culture bases; the top side of the culture dish body is provided with a breathing device; the culture dish body is provided with a liquid conveying port, and the liquid conveying port is arranged at the front edge and the rear edge near the top of the culture dish body and is positioned above the first-layer culture base.
In each layer of culture layer, the periphery of the culture base is enclosed by the inner wall of the culture dish body and the overflow plate to form a periphery-sealed space. After the culture medium conveyed to the culture base at the upper layer overflows the overflow plate, the culture medium flows from the overflow hole to the culture base at the lower layer, and sequentially overflows layer by layer to spread the upper layer of the culture medium layer by layer.
The culture dish for the solid culture medium is mainly used for laboratory experiments of small tests and pilot tests. When the culture medium is used, the solid culture medium is heated and stirred into a liquid state, then the sterilized liquid culture medium is conveyed into the culture dish from the liquid conveying port, overflows to the second layer after the culture medium overflows the first layer of culture base along with the increase of conveying capacity, and then overflows layer by layer in sequence, and the culture medium with fixed thickness is formed on each layer of culture base. After the culture medium on the culture base is solidified, the bacterial liquid is conveyed into the culture dish through the liquid conveying port, and the bacterial liquid is paved on the solid culture medium layer by layer in sequence in the same way, and then the culture dish is placed under the culture condition for culturing for a specified time. After the culture is completed, the culture dish is gently shaken back and forth to separate bacteria from the culture medium, then the front side or the rear side of the culture dish is placed upside down, the liquid conveying port is arranged at or near the lowest point by tilting the culture dish, and bacterial liquid is discharged from the liquid conveying port, so that the bacterial culture operation is completed.
In the present utility model, the culture layer in the dish is 2-10 layers, preferably 3-5 layers, and is selected according to the need and the convenience of operation. The material of the culture dish is any one of organic plastic and glass material. The culture dish can be used as a disposable dish when organic plastic is selected, and can be used as a reusable dish when glass is selected. When selecting the glass culture dish, the glass culture dish can be heated under the heating condition when being recovered, so that the solid culture medium is melted and then discharged from the liquid conveying port.
According to the culture dish for the solid culture medium, preferably, the bottom of the culture dish body is provided with scales for measuring the thickness of the culture medium at the bottom of the culture dish body.
The bottom of the culture dish body is used as the last layer of culture layer, a culture medium with a certain thickness can be paved, and the scale is used for measuring the thickness of the culture medium at the bottom of the culture dish body.
According to the culture dish for the solid culture medium, preferably, the top side of the culture dish body is provided with the opening, the opening is provided with the sealing cover, and the breathing device is arranged on the sealing cover.
In the utility model, the design of the sealing cover can ensure the sealing condition during culture and provide an operation space during cleaning and other operations.
According to the culture dish for the solid culture medium, preferably, the top of each overflow plate is provided with a baffle plate, and the baffle plates are arranged to extend towards the inside of the corresponding culture base.
In the utility model, the fixed culture medium can be further fixed on the culture base by arranging the baffle plate, so that the solid culture medium is prevented from falling off from the culture base when the culture dish is shaken. Preferably, the baffle extends into the culture base for a length of 0.3-1cm.
According to the culture dish for the solid culture medium, preferably, the breathing device is a membrane breathing valve.
In addition, the utility model also provides a culture device, which comprises the culture dish for the solid culture medium.
According to the culture device, preferably, the culture device further comprises a first liquid storage tank, a second liquid storage tank, a third liquid storage tank and a power transmission device, wherein the first liquid storage tank is provided with a heating and stirring device; the outlets of the first liquid storage tank and the second liquid storage tank are connected with the liquid conveying port on the culture dish through the power conveying device; the inlet of the third liquid storage tank is connected with the liquid conveying port through a power conveying device; the power conveying device is arranged to be capable of conveying materials in the first liquid storage tank and the second liquid storage tank into the culture dish respectively and conveying materials in the culture dish into the third liquid storage tank.
In the utility model, a sterilizing device is also arranged in the first liquid storage tank. The sterilization device is used for sterilizing the solid culture medium in the first liquid storage tank.
The culture device of the utility model adopts a first liquid storage tank and a second liquid storage tank to store culture medium and bacterial liquid respectively. Heating and melting the solid culture medium into liquid through the first liquid storage tank, conveying the liquid culture medium to the culture dish through the power conveying device, spreading the liquid culture medium layer by layer on the culture base according to the method, conveying the bacterial liquid in the second liquid storage tank into the culture dish through the power conveying device after solidification, spreading the bacterial liquid layer by layer, and then culturing for a certain time under the culture condition. After the culture is completed, the culture dish is gently shaken back and forth to separate bacteria from the culture medium, then the front side or the rear side of the culture dish is placed upside down, the culture dish is inclined to enable the liquid conveying port to be placed at or near the lowest point, and bacterial liquid is conveyed from the liquid conveying port to the third liquid storage tank through the power conveying device, so that the bacterial culture operation is completed. In the utility model, the power transmission device can be a plurality of transmission pumps or a peristaltic pump with a bidirectional transmission function.
In the utility model, the pipelines between each liquid storage tank and the power transmission device and between the power device and the culture dish can be connected by adopting hoses, and the hoses can be connected by adopting a tee joint with a male port and a female port. Hose clamps are arranged on the hoses at the outlet of each liquid storage tank and the hoses connected with the liquid conveying ports. The device has the advantages of simple structure, low cost and convenient operation, and is suitable for laboratory pilot-scale and pilot-scale use.
According to the culture device, preferably, a plurality of culture dishes are connected in parallel with the power transmission device.
According to the utility model, a plurality of culture dishes are connected in parallel as required, so that the number of one-time culture can be increased, and the working efficiency is improved.
According to one culture device of the utility model, preferably, the power transmission device is a peristaltic pump with a backflow mode.
In the utility model, the peristaltic pump with the bidirectional conveying function can be used as an input pump of a culture dish or an output pump of the culture dish.
The beneficial effects of the utility model are as follows:
the culture dish for the solid culture medium is characterized in that a plurality of culture layers are sequentially arranged in one culture dish body from top to bottom, so that the culture can be performed simultaneously, the occupied area is small, the culture quantity is large, and the required operators are greatly reduced.
Furthermore, as a better technical scheme of the utility model, the utility model also provides a culture device which has the advantages of simple structure, low cost and convenient operation, and is suitable for laboratory experiments and pilot tests.
Drawings
FIG. 1 is a schematic diagram of a culture apparatus according to the present utility model.
FIG. 2 is a schematic diagram showing the structure of a culture dish for a solid medium according to the present utility model.
FIG. 3 is a schematic diagram showing the structure of a culture dish for a solid medium according to the present utility model.
Wherein: 1-culture dish, 2-third hose clamp, 3-peristaltic pump, 4-first hose clamp, 5-second hose clamp, 6-first liquid storage pot, 7-second liquid storage pot, 8-fourth hose clamp, 9-third liquid storage pot, 11-culture dish body, 12-sealed lid, 13-culture base, 14-overflow plate, 15-membrane breather valve, 16-baffle, 17-scale, 18-liquid delivery mouth.
Detailed Description
The technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings.
Example 1
As shown in fig. 2, a culture dish 1 for a solid medium of the present utility model comprises a rectangular parallelepiped culture dish body 11; three layers of culture layers are arranged in the culture dish body 11, each layer of culture layer is provided with a culture base 13, an overflow plate 14 and overflow holes, each layer of culture layer divides the culture base 13 and the overflow holes into a left-right distribution structure through the overflow plate 14, and the overflow holes of the upper layer of culture layer are vertically arranged above the culture base 13 of the lower layer of culture layer; the overflow plates 14 serve to define the thickness of the culture medium on the respective culture base 13;
the bottom of the culture dish body 11 is provided with a scale 17 for measuring the thickness of the culture medium at the bottom of the culture dish body 11; the top side of the culture dish body 11 is provided with an opening, a sealing cover 12 is arranged at the opening, a liquid conveying port 18 is arranged at the top side of the sealing cover 12, which is close to the front edge of the top of the culture dish body 11, and the liquid conveying port 18 is arranged above the first-layer culture base 13; the sealing cover 12 is also provided with a membrane breather valve 15.
Example 2
As shown in fig. 3, in this embodiment, the other structures are the same as those of embodiment 1 except for the following structures:
the front side of the sealing cover 12, which is close to the front edge of the top of the culture dish body 11, is provided with a liquid conveying port 18, the top of the overflow plate 14 is provided with a baffle plate 16, and the baffle plate 16 extends towards the inside of the corresponding culture base 13.
Example 3
As shown in fig. 1, in this embodiment, the other structures are the same as in embodiment 1 except for the following structures:
a culture device comprising at least three culture dishes 1 according to embodiments 1 or 2, a first liquid storage tank 6, a second liquid storage tank 7, a third liquid storage tank 9 and a peristaltic pump 3 with a backflow mode, wherein the first liquid storage tank 6 is provided with a heating stirring device and a sterilizing device; the outlets of the first liquid storage tank 6 and the second liquid storage tank 7 are connected with the inlet of the peristaltic pump 3 through hoses, and a first hose clamp 4 and a second hose clamp 5 are respectively arranged on the hoses connected with the outlets of the first liquid storage tank 6 and the second liquid storage tank 7; the outlet of the peristaltic pump 3 is respectively connected with the liquid conveying ports 18 of the three culture dishes 1 through hoses to form a parallel connection of the culture dishes 1, and a third hose clamp 2 is arranged on the hose at each liquid conveying port 18; the inlet of the third liquid storage tank 9 is connected with the inlet of the peristaltic pump 3 (outlet in a backflow mode) through a hose, and a fourth hose clamp 8 is arranged on the hose at the inlet of the third liquid storage tank 9; peristaltic pump 3 can respectively carry the material in first liquid storage pot 6 and the second liquid storage pot 7 to in the culture dish 1 through liquid delivery port 18, and can carry the material in the culture dish 1 to in the third liquid storage pot 9 through liquid delivery port 18 under the backward flow mode.
The described embodiments are only some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Claims (9)
1. The culture dish for the solid culture medium is characterized by comprising a cuboid culture dish body; at least two layers of culture layers are arranged in the culture dish body, each layer of culture layer is provided with a culture base, an overflow plate and overflow holes, each layer of culture layer divides the culture base and the overflow holes into left-right distribution structures through the overflow plate, and the overflow holes of the upper layer of culture layers are vertically arranged above the culture bases of the lower layer of culture layers; the overflow plates are used for limiting the thickness of the culture medium on the corresponding culture bases; the top side of the culture dish body is provided with a breathing device; the culture dish body is provided with a liquid conveying port, and the liquid conveying port is arranged at the front edge and the rear edge near the top of the culture dish body and is positioned above the first-layer culture base.
2. The culture dish for the solid culture medium according to claim 1, wherein the bottom of the culture dish body is provided with scales for measuring the thickness of the culture medium at the bottom of the culture dish body.
3. The culture dish for the solid culture medium according to claim 1, wherein an opening is formed in the top side of the culture dish body, a sealing cover is arranged at the opening, and the breathing device is arranged on the sealing cover.
4. A culture dish for solid media according to claim 1, wherein the top of each overflow plate is provided with a baffle plate arranged to extend inwardly of the respective culture foot.
5. A culture dish for solid media according to claim 1, wherein the breathing means is a membrane breathing valve.
6. A culture apparatus, comprising a culture dish for a solid medium according to any one of claims 1 to 5.
7. The culture device according to claim 6, further comprising a first liquid storage tank, a second liquid storage tank, a third liquid storage tank and a power transmission device, wherein the first liquid storage tank is provided with a heating and stirring device; the outlets of the first liquid storage tank and the second liquid storage tank are connected with the liquid conveying port on the culture dish through the power conveying device; the inlet of the third liquid storage tank is connected with the liquid conveying port through a power conveying device; the power conveying device is arranged to be capable of conveying materials in the first liquid storage tank and the second liquid storage tank into the culture dish respectively and conveying materials in the culture dish into the third liquid storage tank.
8. A culture apparatus according to claim 7, wherein a plurality of the culture dishes are connected in parallel with the power transmission device.
9. The culture apparatus of claim 7, wherein the power transmission means is a peristaltic pump with a bi-directional transmission function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223399312.3U CN219342154U (en) | 2022-12-19 | 2022-12-19 | Culture dish and culture device for solid culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223399312.3U CN219342154U (en) | 2022-12-19 | 2022-12-19 | Culture dish and culture device for solid culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219342154U true CN219342154U (en) | 2023-07-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223399312.3U Active CN219342154U (en) | 2022-12-19 | 2022-12-19 | Culture dish and culture device for solid culture medium |
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| Country | Link |
|---|---|
| CN (1) | CN219342154U (en) |
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2022
- 2022-12-19 CN CN202223399312.3U patent/CN219342154U/en active Active
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