CN220999633U - Anaerobic culture solution preparation taking device - Google Patents

Abstract

The utility model discloses an anaerobic culture solution preparation and taking device which comprises a gas storage tank, a culture tank and a material taking mechanism, wherein the culture tank comprises a gas mixing chamber arranged above a partition plate, a preparation chamber arranged below the partition plate, a universal fan arranged above the inside of the gas mixing chamber, a first air pump arranged on the partition plate, a plurality of culture bins arranged at the bottom end of the preparation chamber, a connecting pipe fixedly connected below the partition plate, and a pipeline system fixedly connected with a gas outlet of the first air pump, the pipeline system is connected with the culture bins and a gas inlet of a second air pump arranged at the side of the inner wall of the culture tank, the material taking mechanism comprises a plurality of support frames fixedly connected to the surface of the connecting pipe, a compression mechanism arranged at the lower ends of the support frames, a third air pump is arranged at the upper end of the gas storage tank and connected to the gas mixing chamber through a connecting gas pipe, and a control panel is arranged at the upper end of the culture tank; through automatic control and closed design, the uniformity of gas mixing and the accuracy of culture solution taking are ensured, and the risks of external pollution and accidental injury are reduced.

Description

Anaerobic culture solution preparation taking device

Technical Field

The utility model relates to the technical field of anaerobic bacteria culture, in particular to a device for preparing and taking anaerobic culture solution.

Background

Anaerobic microorganisms are a class of microorganisms that survive and reproduce in the absence of oxygen or hypoxia. Since their living activities are independent of oxygen, they have been widely used in various fields of medicine, agriculture, food, biotechnology, etc. In order to study such microorganisms, scientists need to provide them with a culture condition that mimics their natural living environment, which requires the preparation of specific anaerobic culture solutions.

The existing anaerobic culture solution has some defects in the preparation process: in the laboratory, anaerobic culture fluid preparation is often dependent on manual operations, and oxygen concentration assessment is often based on personal experience, resulting in inefficiency and error-prone judgment. To avoid cross-contamination between different anaerobic culture fluids, laboratories typically culture only a single sample, which cannot meet the need for simultaneous culture of multiple samples. When single samples are produced in single batches, the associated equipment is often exposed to the external environment, which not only increases the risk of contamination, but also is vulnerable to damage from accidental collisions. In addition, in order to cope with specific requirements of various anaerobic culture solutions, a laboratory often uses mixed gases with different proportions to perform oxygen removal on the anaerobic culture solutions, but due to the different mixing proportions of the gases, the concentration proportions of the mixed gases injected into a plurality of culture solutions are uneven, so that the efficiency of experiments or production is affected. In addition, in the traditional anaerobic culture solution taking process, excessive taking can lead to waste, or the culture solution is easy to be polluted because of opening the culture container.

Therefore, the utility model provides a device for preparing and taking anaerobic culture solution.

Disclosure of utility model

In order to solve the technical problems, the utility model designs a device for preparing and taking anaerobic culture solution, which solves the problems in the background technology.

In order to achieve the technical effects, the utility model is realized by the following technical scheme: an anaerobic culture solution preparation and taking device comprises an air storage tank, an incubator and a material taking mechanism.

The incubator comprises a gas mixing chamber arranged above the partition plate, a preparation chamber arranged below the partition plate, a universal fan arranged above the inside of the gas mixing chamber, a first air pump arranged on the partition plate, a plurality of culture bins arranged at the bottom end of the preparation chamber, a connecting pipe fixedly connected below the partition plate, and a pipeline system fixedly connected with an air outlet of the first air pump, wherein the pipeline system is connected with the culture bins and an air inlet of a second air pump arranged on the side of the inner wall of the incubator; the material taking mechanism comprises a plurality of supporting frames fixedly connected to the surface of the connecting pipe and a compression mechanism arranged at the lower ends of the supporting frames; the upper end of the gas storage tank is provided with a third air pump, and the air outlet of the third air pump is connected to the gas mixing chamber through a connecting air pipe; the incubator upper end is equipped with control panel, and the lower extreme is equipped with a plurality of fluid-discharge tubes.

Further, the box shell of the incubator is divided into an upper box shell and a lower box seat, and the upper box shell and the lower box seat are connected in a detachable mode.

Further, a plurality of gas bins are arranged in the gas storage box; the air bins are fixedly connected with a connecting sleeve arranged on the air inlet of the third air pump through a third air pipe; the third air pipe is provided with a first electromagnetic valve and a flow regulator.

Further, the pipeline system comprises a collecting pipe fixedly connected to the air outlet of the first air pump, a plurality of first air pipes fixedly connected to the bottom end of the collecting pipe and connected to the culture bin, and a second air pipe fixedly connected to the side of the collecting pipe and connected to the air inlet of the second air pump; the collecting pipe is provided with a second electromagnetic valve; a third electromagnetic valve is arranged on each first air pipe; and a fourth electromagnetic valve is arranged on the second air pipe.

Further, the support frame is in a right-angle structure; the compression mechanism comprises an electric telescopic rod fixedly connected to the lower end of the support frame, and the lower end of the electric telescopic rod penetrates into the culture bin and is fixedly connected with the pressing plate; the pressing plate is in sliding connection in the culture bin.

Further, the upper end of the culture bin is detachably provided with a sealing bin cover.

Further, the air outlet of the second air pump is fixedly connected with an exhaust pipe, and the exhaust pipe extends out of the incubator; the exhaust pipe is provided with a first vacuum gauge, and the outermost end of the exhaust pipe is provided with a fifth electromagnetic valve.

Further, a second vacuum gauge is arranged on one side of the air mixing chamber.

Further, a plurality of fluid-discharge pipes are connected to the culture bin, a pressure sensor is arranged on each fluid-discharge pipe, and a sixth electromagnetic valve is arranged at the outer end of each fluid-discharge pipe.

Further, the control panel is electrically connected with the universal fan, the first air pump, the second air pump, the third air pump, the flow regulator, the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve, the fourth electromagnetic valve, the first vacuum meter, the fifth electromagnetic valve, the pressure sensor, the sixth electromagnetic valve and the second vacuum meter; the control panel is internally provided with a control unit and a timer, and the outer surface of the control panel is provided with a touch screen panel.

The beneficial effects of the utility model are as follows:

1. In the utility model, a plurality of gas bins and related components such as the air sucking pump, the electromagnetic valve, the vacuum gauge and the like ensure that the gas can be timely adjusted in parameters and injected according to the preset proportion and flow, and simultaneously ensure the effective discharge of residual gas and ensure the accuracy and stability of the culture process; through control panel, the user can the automatic control gas mixing with get the process of liquid, greatly reduced manual operation's not enough and error. Particularly, the taking mechanism is used, so that the taking of the culture solution is more accurate, and the waste caused by excessive taking and the risk of pollution of the culture solution caused by opening the container are avoided;

2. In the gas mixing chamber, the rotation of the universal fan helps the mixed gases with different proportions to be fully and uniformly mixed in a closed space, so that the gas with the same concentration proportion can be filled in each culture bin, and an ideal environment is created for subsequent anaerobic culture;

3. The incubator provides a closed environment for the culture solution and is isolated from the external environment, so that the pollution risk caused by external factors is greatly reduced; and also provides protection for the culture fluid so that the culture fluid is not easily damaged by accidental collision, dumping or external force.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of the overall structure of the present utility model;

FIG. 2 is a top view of the present utility model;

FIG. 3 is a schematic view of the utility model taken along line A-A of FIG. 2;

FIG. 4 is an enlarged schematic view of the structure of FIG. 3A in accordance with the present utility model;

FIG. 5 is a front view of the present utility model;

FIG. 6 is a schematic view of the utility model taken at B-B in FIG. 5;

in the drawings, the list of components represented by the various numbers is as follows:

1. A gas storage tank; 11. a third air pump; 111. connecting sleeves; 12. connecting an air pipe; 13. a gas bin; 14. a third air pipe; 15. a first electromagnetic valve; 16. a flow regulator; 2. an incubator; 201. an upper case; 202. a lower box seat; 21. a gas mixing chamber; 211. a second vacuum gauge; 22. a preparation chamber; 23. a universal fan; 24. a first air pump; 25. a culture bin; 26. a connecting pipe; 27. a pipeline system; 271. a collecting pipe; 2711. a second electromagnetic valve; 272. a first air tube; 2721. a third electromagnetic valve; 273; a second air pipe; 2731. a fourth electromagnetic valve; 28. a second air pump; 281. an exhaust pipe; 2811. a first vacuum gauge; 2812. a fifth electromagnetic valve; 3. a material taking mechanism; 31. a support frame; 32. a compression mechanism; 321. an electric telescopic rod; 322. a pressing plate; 4. a partition plate; 5. a control panel; 6. a liquid discharge pipe; 61. a pressure sensor; 62. and a sixth electromagnetic valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1

Referring to fig. 1 to 6, an anaerobic culture solution preparing and taking device includes a gas storage tank 1, an incubator 2 and a material taking mechanism 3, and is characterized in that: the incubator 2 comprises a gas mixing chamber 21 arranged above the partition plate 4, a preparation chamber 22 arranged below the partition plate 4, a universal fan 23 arranged above the inside of the gas mixing chamber 21, a first air pump 24 arranged on the partition plate 4, a plurality of culture bins 25 arranged at the bottom end of the preparation chamber 22, a connecting pipe 26 fixedly connected below the partition plate 4, a pipeline system 27 fixedly connected with the air outlet of the first air pump 24, wherein the pipeline system 27 is connected with the culture bins 25 and the air inlet of a second air pump 28 arranged at the side of the inner wall of the incubator 2; the material taking mechanism 3 comprises a plurality of supporting frames 31 fixedly connected to the surface of the connecting pipe 26 and a compression mechanism 32 arranged at the lower ends of the supporting frames 31; the upper end of the gas storage tank 1 is provided with a third air pump 11, and the air outlet of the third air pump 11 is connected to a gas mixing chamber 21 through a connecting air pipe 12; the upper end of the incubator 2 is provided with a control panel 5, and the lower end is provided with a plurality of liquid discharge pipes 6.

The incubator 2 and the culture bin 25 are made of transparent materials, so that the condition of the culture solution can be conveniently observed. The box shell of the incubator 2 is divided into an upper box shell 201 and a lower box seat 202, the upper box shell 201 and the lower box seat 202 are connected in a detachable mode, the culture solution is conveniently placed in the culture bin 25, and other mechanisms inside the incubator 2 are conveniently cleaned. The upper case 201 and the lower case 202 can be detachably connected by a fastening mechanism, a screw mechanism or the like.

The inside of the gas storage tank 1 is provided with a plurality of gas bins 13. The gas chamber 13 may be filled with various oxygen-discharging gases such as carbon dioxide, hydrogen, nitrogen, argon, etc. The plurality of air bins 13 are fixedly connected with a connecting sleeve 111 arranged on the air inlet of the third air pump 11 through a third air pipe 14. The third air pipe 14 is provided with a first electromagnetic valve 15 and a flow regulator 16. The flow regulator 16 is a gas flow regulator, and can control the third pump 11 to pump out a specific amount of gas. The third air pipe 14 is provided with a first electromagnetic valve 15. The first solenoid valve 15 can select a desired gas under the control of the control panel.

The pipeline system 27 comprises a collecting pipe 271 fixedly connected with the air outlet of the first air pump 24, a plurality of first air pipes 272 fixedly connected with the bottom end of the collecting pipe 271 and connected with the culture bin 25, and a second air pipe 273 fixedly connected with the side of the collecting pipe 271 and connected with the air inlet of the second air pump 28; the collecting pipe 271 is provided with a second electromagnetic valve 2711; the first air pipes 272 are provided with third electromagnetic valves 2721; a fourth solenoid valve 2731 is provided on the second air tube 273.

The supporting frame 31 is in a right-angle structure; the compression mechanism 32 comprises an electric telescopic rod 321 fixedly connected to the lower end of the support frame, and the lower end of the electric telescopic rod 321 penetrates into the culture bin 25 and is fixedly connected with a pressing plate 322; the pressing plate 322 is slidably connected inside the culturing chamber 25.

The upper end of the culture bin 25 is detachably provided with a sealing bin cover. The sealed bin cover can prevent the culture solution in the culture bin 25 from splashing and prevent the environment outside the bin from polluting the culture solution.

The air outlet of the second air pump 28 is fixedly connected with an air exhaust pipe 281, and the air exhaust pipe 281 extends out of the incubator 2; the exhaust pipe 281 is provided with a first vacuum gauge 2811, and the outermost end of the exhaust pipe 281 is provided with a fifth electromagnetic valve 2812.

A second vacuum gauge 211 is provided on one side of the mixing chamber 21.

A plurality of liquid discharge pipes 6 are connected to the culture bin 25, a pressure sensor 61 is arranged on the liquid discharge pipe 6, and a sixth electromagnetic valve 62 is arranged at the outer end of the liquid discharge pipe 6.

The control panel 5 is electrically connected with the universal fan 23, the first air pump 24, the second air pump 28, the third air pump 11, the flow regulator 16, the first electromagnetic valve 15, the second electromagnetic valve 2711, the third electromagnetic valve 2721, the fourth electromagnetic valve 2731, the first vacuum gauge 2811, the fifth electromagnetic valve 2812, the pressure sensor 61, the sixth electromagnetic valve 62 and the second vacuum gauge 211; the control panel 5 is internally provided with a control unit and a timer, and the outer surface is provided with a touch screen panel. The timer and the touch screen panel are electrically connected to the control unit.

Example 2

The operation process and principle of the utility model are as follows:

The user may put the same culture medium into the culture chambers 25 for a large amount of culture, or put different culture mediums into a plurality of culture chambers 25. The touch screen panel of the control panel 5 is opened and the main interface of the system is entered. And setting parameters such as the required gas proportion, flow and the like on the touch screen. If a timed incubation is desired, the start and end times of the incubation can be set by a timer function. After the user confirms the setting, the control panel is operated to send an instruction to the third air pump 11, and one gas (carbon dioxide, hydrogen, nitrogen, or the like) is selected from the air tank 1 to inflate the culture solution in the culture bin 25. Under the control of the control panel 5, the first electromagnetic valve 15, the second electromagnetic valve 2711 and the third electromagnetic valve 2721 are opened, the fourth electromagnetic valve 2731 is closed, gas is conveyed into the gas mixing chamber 21 through the connecting gas pipe 12 and directly pumped out by the first air pump 24 through the collecting pipe 271 and the first gas pipe 272 to enter the culture chamber 25, then the air discharging function is selected by operating the control panel 5, the first electromagnetic valve 15, the second electromagnetic valve 2711 and the third electromagnetic valve 2721 are closed, the fourth electromagnetic valve 2731 and the fifth electromagnetic valve 2812 are opened, and the second air pump 28 pumps out the gas in the culture chamber 25. Such a gas charging and discharging process is performed 2 to 3 times to discharge the oxygen in the culture chamber 25, and then it is determined whether the culture chamber 25 and the gas mixing chamber 21 reach a vacuum state by observing the first vacuum gauge 2811 and the second vacuum gauge 211. If the same anaerobic culture solution is prepared, the same gas or the same mixed gas is used for oxygen discharge according to the requirement. If the anaerobic culture liquid is prepared for a plurality of anaerobic culture liquids, the corresponding gas can be selected by controlling the switch between the electromagnetic valves so as to realize the preparation of different anaerobic culture liquids.

After the oxygen is exhausted, various gases (e.g., 80% ammonia, 10% hydrogen, and 10% carbon dioxide) are then extracted at a predetermined ratio. The mixed gas is supplied to the gas mixing chamber 21 through the connecting gas pipe 12. In the air mixing chamber, the universal fan 23 is automatically started and can be set at a timing as required to perform omnibearing blowing so as to accelerate the movement among gas molecules and ensure that the gases with different proportions are fully mixed.

The mixed gas is injected into the plurality of culture bins 25 in the preparation chamber 22 via the first air pump 24. The culture solution is cultured in the mixed gas environment of injection.

After the completion of the culture, the culture medium may be stored in the culture room 25 without oxygen. The user can automatically take the culture solution according to preset parameters. The user clicks the function of taking the culture solution on the touch screen, and selects the taking amount, and the system automatically starts the taking mechanism 3. The electric telescopic rod 321 of the compression mechanism 32 drives the pressing plate 322 to move downward, pressure is applied to the culture solution in the culture bin, the culture solution does not flow out when the culture solution is pressurized due to the existence of gas in the culture solution, and when the electric telescopic rod 321 compresses the gas and contacts the liquid surface, the sixth electromagnetic valve 62 is opened at this time to allow the culture solution to flow out from the liquid discharge pipe 6 when the threshold value set by the pressure sensor 61 is reached. The threshold value set by the pressure sensor 61 needs to be determined experimentally to ensure that the culture medium flows out only after the gas is fully compressed.

The utility model ensures the uniformity of gas mixing and the accuracy of culture solution taking through automatic control and closed design, and reduces the risks of external pollution and accidental injury.

Claims (10)

1. The utility model provides a device is taken in anaerobic culture liquid preparation, includes gas receiver (1), incubator (2) and extracting mechanism (3), its characterized in that: the incubator (2) comprises a gas mixing chamber (21) arranged above the partition plate (4) and a preparation chamber (22) arranged below the partition plate (4), a universal fan (23) arranged above the inside of the gas mixing chamber (21), a first air pump (24) arranged on the partition plate (4), a plurality of culture bins (25) arranged at the bottom end of the preparation chamber (22), connecting pipes (26) fixedly connected under the partition plate (4), and a pipeline system (27) fixedly connected with the air outlet of the first air pump (24), wherein the pipeline system (27) is connected with the air inlets of the culture bins (25) and a second air pump (28) arranged at the side of the inner wall of the incubator (2); the material taking mechanism (3) comprises a plurality of supporting frames (31) fixedly connected to the surface of the connecting pipe (26) and a compression mechanism (32) arranged at the lower ends of the supporting frames (31); the upper end of the gas storage tank (1) is provided with a third air pump (11), and the air outlet of the third air pump (11) is connected to the gas mixing chamber (21) through a connecting air pipe (12); the upper end of the incubator (2) is provided with a control panel (5), and the lower end of the incubator is provided with a plurality of liquid discharge pipes (6).

2. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that the casing of the incubator (2) is divided into an upper casing (201) and a lower casing (202), and the upper casing (201) and the lower casing (202) are detachably connected.

3. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that a plurality of gas bins (13) are arranged inside the gas storage tank (1); the plurality of air bins (13) are fixedly connected with a connecting sleeve (111) arranged on the air inlet of the third air pump (11) through a third air pipe (14); the third air pipe (14) is provided with a first electromagnetic valve (15) and a flow regulator (16).

4. The anaerobic culture fluid preparation and taking device according to claim 1, wherein the pipeline system (27) comprises a collecting pipe (271) fixedly connected with an air outlet of the first air pump (24), a plurality of first air pipes (272) fixedly connected with the bottom end of the collecting pipe (271) and connected with the culture bin (25), and a second air pipe (273) fixedly connected with the side of the collecting pipe (271) and connected with an air inlet of the second air pump (28); a second electromagnetic valve (2711) is arranged on the collecting pipe (271); the first air pipes (272) are provided with third electromagnetic valves (2721); a fourth electromagnetic valve (2731) is arranged on the second air pipe (273).

5. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that said support (31) is of right-angle construction; the compression mechanism (32) comprises an electric telescopic rod (321) fixedly connected to the lower end of the support frame, and the lower end of the electric telescopic rod (321) penetrates into the culture bin (25) and is fixedly connected with the pressing plate (322); the pressing plate (322) is in sliding connection with the inside of the culture bin (25).

6. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that the upper end of the culture bin (25) is detachably provided with a sealing bin cover.

7. The anaerobic culture fluid preparation and taking device according to claim 1, wherein the air outlet of the second air pump (28) is fixedly connected with an air exhaust pipe (281) and the air exhaust pipe (281) extends out of the incubator (2); the exhaust pipe (281) is provided with a first vacuum gauge (2811), and the outermost end of the exhaust pipe (281) is provided with a fifth electromagnetic valve (2812).

8. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that a second vacuum gauge (211) is arranged on one side of the air mixing chamber (21).

9. Anaerobic culture fluid preparation and taking device according to claim 1, characterized in that a plurality of said fluid-discharge pipes (6) are connected to the culture bin (25), a pressure sensor (61) is provided on said fluid-discharge pipe (6), and a sixth electromagnetic valve (62) is provided at the outer end of the fluid-discharge pipe (6).

10. The anaerobic culture fluid preparation and taking device according to claim 1, wherein the control panel (5) is electrically connected with a universal fan (23), a first air pump (24), a second air pump (28), a third air pump (11), a flow regulator (16), a first electromagnetic valve (15), a second electromagnetic valve (2711), a third electromagnetic valve (2721), a fourth electromagnetic valve (2731), a first vacuum gauge (2811), a fifth electromagnetic valve (2812), a pressure sensor (61), a sixth electromagnetic valve (62) and a second vacuum gauge (211); the control panel (5) is internally provided with a control unit and a timer, and the outer surface of the control panel is provided with a touch screen panel.