CN213266493U

CN213266493U - Sampling device

Info

Publication number: CN213266493U
Application number: CN202021755794.XU
Authority: CN
Inventors: 范琳琳; 杨朝晖; 任涛; 张明华; 何佳琦; 唐秀丽; 胡鹏; 李向阳; 刘幸; 李鑫; 樊溢; 钱伟
Original assignee: Beijing Minhai Biotechnology Co ltd
Current assignee: Beijing Minhai Biotechnology Co ltd
Priority date: 2020-08-20
Filing date: 2020-08-20
Publication date: 2021-05-25
Anticipated expiration: 2030-08-20

Abstract

The utility model provides a sampling device, which controls the sampling volume from a fermentation tank to an intermediate sampler, then distributes the sampling volume to a selected sampling tube through a third flexible tube and a switch part on the third flexible tube, and seals the third flexible tube by the switch part after taking a certain amount of liquid; after sampling, discharging the residual liquid into a liquid storage device through a second flexible pipe to finish primary sampling; the sampling of multiple batches can be completed by repeating the steps, so that the sampling volume is controlled, and in the sampling process of multiple batches, the chuck is kept to be closed with the sampling port of the fermentation tank, so that the phenomenon that the sampling port of the fermentation tank is exposed in the air due to the traditional sampling of multiple batches is avoided, and the risk of pollution to the culture of the fermentation tank is reduced.

Description

Sampling device

Technical Field

The utility model relates to a microbial cultivation field specifically is a sampling device that when microbial cultivation, carries out the sample to the fermentation cylinder culture medium.

Background

In real life, there are many microorganisms such as fungi, bacteria and viruses, which play a very important role although they are small in size and invisible to the naked eye. Microbial fermentation refers to a process of converting a raw material into a product required by a human being through a specific metabolic pathway by using a microorganism under an appropriate condition. With the development of scientific research, microbial fermentation engineering has been widely applied to various fields of human life and plays an important role, such as pharmaceutical industry, food industry, energy industry, chemical industry, agriculture and environmental protection.

In the fermentation process, it is necessary to detect the cell concentration and observe the growth state of the cells. Therefore, we need to take multiple batches from the fermentation system for testing. In industrial production, the fermentation system used is large in volume and cannot be used for sampling in a biological safety cabinet like small-volume culture. If the sample is taken directly from the sampling port of the fermenter, the sampling volume is not easy to control, and the contamination of the culture is easily caused during the repeated sampling of many batches.

To facilitate the sampling of multiple batches from a large fermentation system and to reduce contamination of the culture during sampling, a device is provided that allows for the sampling of multiple batches of culture.

Disclosure of Invention

An object of the utility model is to provide a sampling device, include:

the liquid sampler comprises a chuck 1 used for clamping with a liquid outlet, and an intermediate sampler 3 connected with the chuck 1 through a first flexible pipe 2, wherein the intermediate sampler 3 is used for preliminarily storing and distributing liquid taken out from the liquid outlet;

a liquid reservoir 4 communicating with the intermediate sampler 3 through a second flexible tube 41, the liquid reservoir 4 including a first breather 42 for keeping a pressure difference inside and outside the liquid reservoir 4 balanced;

the intermediate sampler 3 includes: a chamber 31 for preliminarily storing the liquid taken out from the liquid discharge port, and a guide tube 33 connected to the first flexible tube 2 and introduced into the inside of the chamber 31; a plurality of sampling interfaces 32 arranged around the cavity 31, wherein the sampling interfaces 32 are connected with a sampling tube 38 or a second respirator 36 or a plug 37 through a third flexible tube 35; a drain pipe 34 disposed opposite to the guide pipe 33, the drain pipe 34 being used for discharging the liquid after sampling;

and the switch piece is used for controlling the opening or closing of the first flexible pipe 2, the second flexible pipe 41 and the third flexible pipe 35.

The sampling device controls the sampling volume from the fermentation tank to the intermediate sampler 3, then distributes the sampling volume to the selected sampling tube 38 through the third flexible tube 35 and the switch piece on the third flexible tube, and closes the third flexible tube 35 by the switch piece after taking a certain amount of liquid; after sampling is finished, discharging the residual liquid into the liquid storage device 4 through the second flexible pipe 41 to finish the first sampling; the sampling of multiple batches can be accomplished to the repetition above-mentioned step to reach control sample volume, in the sampling process of multiple batches, chuck 1 keeps airtight with the fermentation cylinder sample connection, has avoided traditional multiple batches to sample and has made the fermentation cylinder sample connection expose in the air, has reduced the risk to fermentation cylinder culture pollution.

The rigid parts are connected by flexible tubing to provide a flexible connection between them, and the sampler 3 can be tilted to allow fluid to flow into a selected sampling port 32 as fluid is dispensed into the selected sampling port 32. The sampling interface 32 which needs to be sampled is connected with the sampling tube 38, and the sampling interface 32 which does not need to be sampled is connected with the plug 37 and closed.

Preferably, the middle sampler 3 is a cylinder with arc tops at two ends;

the guide pipe 33 is arranged at the top of the intermediate sampler 3, and the drain pipe 34 is arranged at the bottom of the intermediate sampler 3;

the sampling ports 32 are distributed in the middle upper portion of the intermediate sampler 3.

By this distribution, the liquid taken out of the fermenter is allowed to flow by its own weight directly from the guide tube 33 to the bottom of the intermediate sampler 3, preventing uncontrolled inflow into the sampling connection 32.

Preferably, the second breather 36 is connected to the sampling port 32 near the top.

With such an arrangement, the breather of the intermediate sampler 3 can be arranged at the top of the intermediate sampler 3 as much as possible, and the sampling interface 32 where the breather is located is prevented from being splashed by liquid.

Preferably, the liquid reservoir 4 comprises a cover body 43 for sealing, the cover body 43 is provided with a first breather 42 in sealed communication with the liquid reservoir 4, and a connecting pipe 44 in sealed communication with the liquid reservoir 4, and the connecting pipe 44 is used for connecting with a second flexible pipe 41.

Preferably, the first flexible tube 2, the second flexible tube 41, and the third flexible tube 35 are silicone tubes; the liquid storage device 4 is a reagent bottle; the switch piece is a hemostatic forceps.

It should be understood that the above components may be selected according to the situation of a specific laboratory, the common flexible tube may be a silicone tube, the liquid reservoir 4 may be a reagent bottle with a suitable volume, and the connection tube 44 may be a glass tube, stainless steel, ceramic, or other suitable plastic materials.

Drawings

Fig. 1 is a schematic view of a sampling device in embodiment 1 of the present invention.

The device comprises a chuck 1, a chuck 2, a first flexible pipe, a middle sampler 3, a cavity 31, a sampling interface 32, a guide pipe 33, a liquid discharge pipe 34, a third flexible pipe 35, a second breather 36, a choke plug 37, a sampling pipe 38, a liquid storage device 4, a second flexible pipe 41, a first breather 42, a cover 43 and a connecting pipe 44.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present invention, the present invention will be further described in detail with reference to the following detailed description.

Example 1:

as shown in fig. 1, the present embodiment provides a sampling device, including: the chuck 1 is used for being clamped with a liquid outlet of the fermentation tank, and the chuck 1 can be made of stainless steel;

an intermediate sampler 3 connected to the cartridge 1 through a first flexible tube 2, the intermediate sampler 3 being for preliminarily storing and distributing the liquid taken out from the liquid discharge port;

and a switch member (not shown) for controlling the first flexible tube 2, the second flexible tube 41 and the third flexible tube 35 to be opened or closed. It should be understood that other than flexible tubing, other tubing may be made of glass.

Preferably, the middle sampler 3 is a cylinder with arc tops at two ends;

It should be understood that the above components can be selected according to specific situations, common flexible tubes can be selected from silicone tubes, the liquid reservoir 4 can be selected from a reagent bottle with a suitable volume, the connection tube 44 can be selected from a glass tube, stainless steel, ceramic or other suitable plastic materials, the selection of the above components and materials is adapted to sterilization conditions, and the volumes of the test tubes and the containers can be selected according to actual situations, so as to match the application environment and ensure the tightness and reliability of connection.

The sampling device comprises the following steps:

(1) sealing and completely connecting all parts of the sampling device; the third flexible tube 35 (silicone tube) connected with the sampling tube 38 and the second flexible tube 41 are all closed by hemostatic forceps; the fermentation tank and the sampling device are connected by a stainless steel chuck 1.

(2) After opening the sampling port valve of the fermentation tank, the culture solution enters the sampling device, and the sampling port valve of the fermentation tank is closed approximately to the sampling amount.

(3) The hemostat on the selected sampling tube 38 is opened, the intermediate sampler 3 is tilted to make the culture solution enter the selected sampling tube 38 from the sampling interface 32, then the third flexible tube 35 (silicone tube) where the selected sampling tube 38 is located is clamped by a hemostat, and the first sampling is completed by cutting between the two hemostats by scissors. And repeating the steps to finish sampling of multiple samples.

(4) The hemostat on the second flexible tube 41 (silicone tube) is opened and excess culture is drained into the reservoir 4 (reagent bottle).

And (4) repeating the steps (2), (3) and (4) to complete sampling of multiple batches.

Claims

1. A sampling device, comprising:

a chuck used for being clamped with the liquid outlet,

An intermediate sampler connected to the cartridge through a first flexible tube, the intermediate sampler for preliminarily storing and dispensing the liquid taken out from the liquid discharge port;

a reservoir in communication with the intermediate sampler via a second flexible tube, the reservoir including a first breather for maintaining a pressure differential balance inside and outside the reservoir;

the intermediate sampler includes: the cavity is used for preliminarily storing the liquid taken out from the liquid outlet, and the guide pipe is connected with the first flexible pipe and is guided into the cavity; the sampling interfaces are arranged around the cavity and are connected with the sampling tube, the second respirator or the plug through a third flexible tube; the liquid discharge pipe is arranged opposite to the guide pipe and used for discharging liquid after sampling is finished;

and the switch piece is used for controlling the first flexible pipe, the second flexible pipe and the third flexible pipe to be opened or closed.

2. The sampling device of claim 1, wherein the intermediate sampler is a cylinder with two curved ends;

the guide pipe is arranged at the top of the intermediate sampler, and the liquid discharge pipe is arranged at the bottom of the intermediate sampler;

the sampling interfaces are distributed on the middle upper part of the intermediate sampler.

3. The sampling device of claim 1, wherein the second breather is connected to the sampling interface near the top.

4. The sampling device of claim 1, wherein the reservoir comprises a cap for sealing, the cap having a first breather in sealed communication with the reservoir, and a connecting tube in sealed communication with the reservoir for connection to a second flexible tube.

5. The sampling device according to any one of claims 1 to 4, wherein said first flexible tube, said second flexible tube, said third flexible tube are silicone tubing; the liquid storage device is a reagent bottle; the switch piece is a hemostatic forceps.