CN216303867U

CN216303867U - Cell continuous perfusion device based on living cell experiment

Info

Publication number: CN216303867U
Application number: CN202121900898.XU
Authority: CN
Inventors: 魏源长; 熊军宾
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-14
Filing date: 2021-08-14
Publication date: 2022-04-15
Anticipated expiration: 2031-08-14

Abstract

The utility model discloses a cell continuous perfusion device based on a living body cell experiment, which comprises a bottom plate and an operation table fixedly arranged on the upper surface of the bottom plate, wherein the upper surface of the operation table is fixedly connected with a base, a perfusion bottle is arranged inside the base, a peristaltic pump is arranged on the upper surface of the operation table, a placing groove is formed in the upper surface of the operation table, a constant temperature device is arranged on one side of the placing groove, a placing frame is arranged inside the placing groove, a culture shell is arranged at the top of the placing frame, and conveying pipes are respectively arranged between the perfusion bottle and the peristaltic pump and between the peristaltic pump and the culture shell. According to the utility model, the internal culture solution of the perfusion bottle is pumped into the culture tank from the bottom of the culture tank through the peristaltic pump, and then the cell waste liquid is discharged into the waste liquid collecting bottle from the overflow tank along the three-way pipe in an overflow mode, so that continuous perfusion of cells is realized, and the problems of overfilling and overflow of the perfusion tank or evacuation of the perfusion tank are effectively solved.

Description

Cell continuous perfusion device based on living cell experiment

Technical Field

The utility model relates to the technical field of cell perfusion culture, in particular to a cell continuous perfusion device based on a living cell experiment.

Background

The perfusion culture is that after cells and a culture medium are added into a reactor together, in the processes of cell growth and product formation, part of conditioned medium is continuously taken out, and simultaneously, a new culture medium is continuously poured.

However, the current perfusion system is set as a speed control system for liquid input and a suction control system for liquid output, and the two systems are not properly matched, which may cause the phenomenon of overflowing of the perfusion tank or evacuation of the perfusion tank.

SUMMERY OF THE UTILITY MODEL

The present invention aims to provide a continuous cell perfusion apparatus based on living cell experiments to solve the problems of the background art.

In order to achieve the purpose, the utility model provides the following technical scheme: a cell continuous perfusion device based on a living body cell experiment comprises a bottom plate and an operation table fixedly installed on the upper surface of the bottom plate, wherein the upper surface of the operation table is fixedly connected with a base, a perfusion bottle is arranged inside the base, a peristaltic pump is installed on the upper surface of the operation table, a placing groove is formed in the upper surface of the operation table, a constant temperature device is installed on one side of the placing groove, a placing frame is arranged inside the placing groove, a culture shell is arranged at the top of the placing frame, conveying pipes are installed between the perfusion bottle and the peristaltic pump and between the peristaltic pump and the culture shell, a culture groove is formed in the culture shell, a liquid inlet pipe is arranged on one side of the bottom of the culture groove, filter membranes are arranged at the end part of the liquid inlet pipe and the top of the culture groove, an overflow groove is arranged on one side of the culture groove, and a three-way pipe is installed at the bottom of the overflow groove, two output ends of the three-way pipe are respectively provided with a control valve, a waste liquid collecting bottle is arranged in the placing rack, and one output end of the three-way pipe is inserted in the waste liquid collecting bottle.

Preferably, the two sides of the bottom plate are fixedly connected with supporting plates, and the bottom of each supporting plate is provided with a protection pad.

Preferably, one side of the operating platform is movably connected with a placing drawer, and a plurality of partition plates are arranged inside the placing drawer.

Preferably, the top of the culture shell is provided with a top cover, and the middle part of the top cover is provided with a transparent observation window.

Preferably, the inside of rack is provided with the cavity, the bottom of cavity is provided with the slipmat.

Preferably, the top of the chamber is provided with a through hole, and the three-way pipe penetrates through the inside of the through hole.

Compared with the prior art, the utility model has the beneficial effects that:

according to the utility model, the culture solution in the perfusion bottle is pumped into the culture tank from the bottom of the culture tank through the peristaltic pump, and then the cell waste liquid is discharged into the waste liquid collecting bottle from the overflow tank along the three-way pipe in an overflow mode, so that the continuous perfusion of cells is realized, the problem that the perfusion tank is overfilled and overflows or the perfusion tank is evacuated is effectively solved, and the replacement operation of the waste liquid collecting bottle is facilitated through the three-way pipe and the two control valves, the waste liquid can be effectively prevented from spilling during the replacement, and the normal work of the whole continuous perfusion system is not influenced.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of the connection structure of the culture shell and the waste liquid collecting bottle according to the present invention;

fig. 3 is a schematic perspective view of the rack of the present invention.

In the figure: 1. a base plate; 2. an operation table; 3. a base; 4. filling a flow bottle; 5. a peristaltic pump; 6. a placement groove; 7. a thermostatic device; 8. placing a rack; 9. culturing the shell; 10. a delivery pipe; 11. a culture tank; 12. a liquid inlet pipe; 13. filtering the membrane; 14. an overflow trough; 15. a three-way pipe; 16. a control valve; 17. a waste liquid collecting bottle; 18. a support plate; 19. a pad; 20. placing a drawer; 21. a top cover; 22. a transparent viewing window; 23. a non-slip mat; 24. and a through hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, an embodiment of the present invention is shown: a cell continuous perfusion device based on a living body cell experiment comprises a bottom plate 1 and an operation table 2 fixedly installed on the upper surface of the bottom plate 1, wherein the upper surface of the operation table 2 is fixedly connected with a base 3, a perfusion bottle 4 is arranged inside the base 3, a peristaltic pump 5 is installed on the upper surface of the operation table 2 and provides conveying power, a placing groove 6 is formed in the upper surface of the operation table 2, a constant temperature device 7 is installed on one side of the placing groove 6, the constant temperature device 7 is reserved on the operation table 2 and can be conveniently used when needed, a placing frame 8 is arranged inside the placing groove 6, a culture shell 9 is arranged on the top of the placing frame 8, conveying pipes 10 are respectively installed between the perfusion bottle 4 and the peristaltic pump 5 as well as between the peristaltic pump 5 and the culture shell 9, a culture tank 11 is formed inside the culture shell 9, a liquid inlet pipe 12 is arranged on one side of the bottom of the culture tank 11, filter membranes 13 are respectively arranged at the end part of the liquid inlet pipe 12 and the top of the culture tank 11, through the setting of filter membrane 13, prevent that the cultured cell from running off along with the culture solution together, one side of the culture trough 11 of filter membrane 13 is provided with overflow launder 14, three-way pipe 15 is installed to overflow launder 14's bottom, two outputs of three-way pipe 15 all are provided with control valve 16, rack 8's inside is provided with waste liquid receiving flask 17, one of them output of three-way pipe 15 is pegged graft in waste liquid receiving flask 17's inside, go into the inside to culture trough 11 from culture trough 11's bottom pump with the inside culture solution of perfusion bottle 4 through peristaltic pump 5, then discharge the inside to waste liquid receiving flask 17 along three-way pipe 15 with the cell waste liquid from overflow launder 14 through the mode of overflow, the continuous perfusion to the cell has been realized.

Furthermore, both sides of the bottom plate 1 are fixedly connected with supporting plates 18, and the bottom of the supporting plate 18 is provided with a protection pad 19, so that the supporting function is achieved.

Further, one side swing joint of operation panel 2 has places drawer 20, and the internally mounted who places drawer 20 has a plurality of baffle, places drawer 20 and is used for placing auxiliary equipment etc. does benefit to get and puts, and the baffle is favorable to equipment, article classification to place.

Further, the top of the culture shell 9 is provided with a top cover 21, and the middle part of the top cover 21 is provided with a transparent observation window 22, which is convenient for observation.

Further, the inside of rack 8 is provided with the cavity, and the bottom of cavity is provided with slipmat 23 for the condition of empting is difficult to appear in the waste liquid receiving flask 17 of placing in the cavity inside.

Furthermore, a through hole 24 is formed at the top of the chamber, and the tee pipe 15 penetrates through the through hole 24.

The working principle is as follows: when the perfusion bottle is used, the culture solution in the perfusion bottle 4 is pumped into the culture tank 11 from the bottom of the culture tank 11 through the peristaltic pump 5, and then the cell waste liquid is discharged into the waste liquid collecting bottle 17 from the overflow groove 14 in an overflow mode along the three-way pipe 15, so that continuous perfusion of cells is realized; moreover, when the waste liquid collecting bottle 17 needs to be replaced, the empty waste liquid collecting bottle 17 is connected with the output end of the three-way pipe 15 in the idle state, the control valve 16 on the output end is opened, then the control valve 16 on the output end of the originally used three-way pipe 15 is closed, the waste liquid collecting bottle 17 filled with waste liquid is taken down, and the corresponding control valve 16 is closed, so that the waste liquid collecting bottle 17 is replaced.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a cell continuous perfusion device based on live body cell experiment, includes bottom plate (1) and fixed mounting in operation panel (2) of bottom plate (1) upper surface which characterized in that: the upper surface of the operating platform (2) is fixedly connected with a base (3), an infusion bottle (4) is arranged inside the base (3), a peristaltic pump (5) is arranged on the upper surface of the operating platform (2), a placing groove (6) is formed in the upper surface of the operating platform (2), a constant temperature device (7) is arranged on one side of the placing groove (6), a placing rack (8) is arranged inside the placing groove (6), a culture shell (9) is arranged at the top of the placing rack (8), conveying pipes (10) are arranged between the infusion bottle (4) and the peristaltic pump (5) and between the peristaltic pump (5) and the culture shell (9), a culture groove (11) is formed in the culture shell (9), a liquid inlet pipe (12) is arranged on one side of the bottom of the culture groove (11), filter membranes (13) are arranged at the end part of the liquid inlet pipe (12) and the top of the culture groove (11), an overflow groove (14) is formed in one side of the culture tank (11), a three-way pipe (15) is installed at the bottom of the overflow groove (14), control valves (16) are arranged at two output ends of the three-way pipe (15), a waste liquid collecting bottle (17) is arranged inside the placing frame (8), and one output end of the three-way pipe (15) is inserted into the waste liquid collecting bottle (17).

2. The continuous cell perfusion device according to claim 1, wherein the continuous cell perfusion device comprises: the supporting plates (18) are fixedly connected to two sides of the bottom plate (1), and protection pads (19) are arranged at the bottoms of the supporting plates (18).

3. The continuous cell perfusion device according to claim 1, wherein the continuous cell perfusion device comprises: one side swing joint of operation panel (2) has places drawer (20), just place the internally mounted of drawer (20) and have a plurality of baffle.

4. The continuous cell perfusion device according to claim 1, wherein the continuous cell perfusion device comprises: the top of the culture shell (9) is provided with a top cover (21), and the middle part of the top cover (21) is provided with a transparent observation window (22).

5. The continuous cell perfusion device according to claim 1, wherein the continuous cell perfusion device comprises: the inside of rack (8) is provided with the cavity, the bottom of cavity is provided with slipmat (23).

6. A continuous perfusion device for cells based on living body cell experiment as claimed in claim 5, wherein: the top of the cavity is provided with a through hole (24), and the three-way pipe (15) penetrates through the inside of the through hole (24).