CN211532517U

CN211532517U - Organ preservation device

Info

Publication number: CN211532517U
Application number: CN201922325104.0U
Authority: CN
Inventors: 董明清; 陈键; 王璞; 刘凯峰
Original assignee: XI'AN DINGHUA ELECTRONICS CO LTD
Current assignee: XI'AN DINGHUA ELECTRONICS CO LTD
Priority date: 2019-12-22
Filing date: 2019-12-22
Publication date: 2020-09-22
Anticipated expiration: 2029-12-22

Abstract

The application provides an organ preservation device, and belongs to the technical field of organ preservation. The organ preservation apparatus includes: the device comprises a transfer tank, a recovery tank, a liquid supplementing assembly, a gas-liquid mixing assembly, a filling assembly, a backflow assembly and a box body; the liquid supplementing assembly is started to supplement nutrient solution to the transfer tank; the gas supplementing assembly is started to supplement nutrient gas to the gas-liquid input end of the gas-liquid mixing assembly; the perfusion assembly is started to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing assembly and perfuse the gas-liquid output by the gas-liquid output end of the gas-liquid mixing assembly into the artery of the organ; the backflow component is started to backflow the liquid in the recovery tank to the transfer tank. The application solves the problem that the preservation time of the organ is to be further improved. The present application is for preserving organs.

Description

Organ preservation device

Technical Field

The application relates to the technical field of organ preservation, in particular to an organ preservation device.

Background

In medical research and clinical procedures involving organs, organs are used and certain preservation techniques are used to preserve the organs.

For example, in the case of organ transplantation, after the donor organ is harvested, the donor organ is preserved to ensure its integrity and health. Currently, organs are generally preserved using cryopreservation techniques or super-cold preservation techniques, which preserve the organ by lowering the temperature of the organ in a manner that reduces the viability of the organ.

However, when such techniques are used for organ preservation, ice crystal damage may be caused to the organ, and organ function damage may be caused by long-term preservation, so that it is not suitable for long-term preservation of the organ, and the preservation time of the current organ preservation techniques needs to be further improved.

SUMMERY OF THE UTILITY MODEL

The application provides an organ preservation device, can solve the problem that the preservation time of organ preservation technique in the correlation technique needs further promotion, technical scheme is as follows:

there is provided an organ preservation apparatus comprising: the device comprises a transfer tank, a recovery tank, a liquid supplementing assembly, a gas-liquid mixing assembly, a filling assembly, a backflow assembly and a box body;

the transfer tank with retrieve the jar and all be located the box, it is used for placing the organ to retrieve the jar, fluid infusion subassembly with transfer tank intercommunication, the gas-liquid input of gas-liquid mixture subassembly with tonifying qi subassembly the transfer tank all communicates, the gas-liquid output of gas-liquid mixture subassembly with the perfusion module intercommunication, the perfusion module still with the artery intercommunication of organ.

Optionally, the organ preservation apparatus further comprises: the transfer liquid level monitoring assembly is fixed on the side wall of the transfer tank.

Optionally, the organ preservation apparatus further comprises: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank and a waste liquid discharging component,

the recovery tank is internally fixedly provided with a mesh enclosure, the mesh enclosure is used for bearing the organs, the nutrient solution monitoring assembly is located in the transfer tank, the recovery liquid level monitoring assembly is fixed on the side wall of the recovery tank, and the waste liquid discharge assembly is communicated with the recovery tank and the waste liquid tank.

Optionally, the nutrient gas comprises oxygen, the organ preservation apparatus further comprising: and the liquid-gas analyzer is communicated with the gas-liquid output end of the gas-liquid mixing component.

Optionally, the box includes: outer container, inner box and heating member, the outer container parcel the inner box, the heating member is located the outer container with between the inner box, be provided with water bath and temperature element in the inner box, the transit jar with retrieve the jar and all place the water bath is internal.

Optionally, the organ preservation apparatus further comprises: the gas-liquid input end of the gas-liquid mixing component is communicated with the transfer tank through the liquid conveying pipe;

the fluid infusion subassembly includes: the first peristaltic pump is communicated with the nutrient solution source through the first liquid extracting pipe and communicated with the transfer tank through the liquid supplementing pipe;

the tonifying qi subassembly includes: the device comprises an air pump, a first flow valve, a second flow valve, a third flow valve, a first air exhaust pipe, a second air exhaust pipe, a third air exhaust pipe, an air inlet pipe, an air supply pipe, an oxygen source and a carbon dioxide source; the air pump is communicated with the first flow valve, the second flow valve and the third flow valve through the air inlet pipe and is communicated with a gas-liquid input end of the gas-liquid mixing assembly through the air supplementing pipe; the first flow valve is communicated with the outside air through the first air exhaust pipe, and the second flow valve is communicated with the oxygen source through the second air exhaust pipe; the third flow valve is communicated with the carbon dioxide source through the third exhaust pipe;

the perfusion assembly comprises: the second peristaltic pump is communicated with the gas-liquid output end of the gas-liquid mixing component through the second liquid extracting pipe, and the second peristaltic pump is communicated with the artery of the organ through the perfusion pipe;

the reflow assembly includes: the third peristaltic pump passes through the third liquid suction pipe with retrieve the jar intercommunication, and through return the liquid pipe with the transfer jar intercommunication.

Optionally, the transit level monitoring assembly includes: the transfer high-order monitoring component is attached in the transfer high-order department of the lateral wall of transfer jar, the transfer low-order monitoring component is attached in the transfer low-order department of the lateral wall of transfer jar.

Optionally, the recovery level monitoring assembly comprises: the recovery high-level monitoring element is attached to the recovery high-level position of the side wall of the recovery tank, and the recovery low-level monitoring element is attached to the recovery low-level position of the side wall of the recovery tank;

the waste liquid discharge assembly includes: the fourth peristaltic pump is communicated with the recovery tank through the fourth liquid pumping pipe and communicated with the waste liquid tank through the liquid discharge pipe.

Optionally, the organ preservation apparatus further comprises: and the gas-liquid output end of the gas-liquid mixing assembly is communicated with the liquid-gas analyzer through the liquid outlet pipe.

Optionally, the third liquid extraction pipe is communicated with the recovery tank at the bottom of the recovery tank, and the liquid return pipe is communicated with the transfer tank at the bottom of the transfer tank.

The beneficial effect that technical scheme that this application provided brought is: in the organ preservation device provided by the embodiment of the utility model, the liquid supplementing component can supplement nutrient solution to the transfer tank, and the gas supplementing component can supplement nutrient gas to the gas-liquid input end of the gas-liquid mixing component, so that after the liquid in the transfer tank is pumped into the gas-liquid input end of the gas-liquid mixing component by the perfusion component, gas-liquid of mixed nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and the gas-liquid is perfused to the organ, so that the organ can perform physiological activities; and, the perfusion module perfuses the organ after a period of time, the nutrient solution can be followed the venous drainage of organ, collect organ exhaust nutrient solution at the recovery jar after, the backward flow subassembly can be with the liquid backward flow of recovery jar in to the transfer jar, make the nutrient solution that the perfusion module can last in with the transfer jar pump the gas-liquid mixed group subassembly, and perfuse the gas-liquid of gas-liquid mixed subassembly's gas-liquid output to the organ, make the organ can carry out physiological motion in longer time span, be favorable to improving the time of preserving the organ.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.

Drawings

In order to illustrate the embodiments of the present invention more clearly, the drawings that are needed in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the description below are only some embodiments of the present invention, and that other drawings can be obtained by those skilled in the art without inventive work.

Fig. 1 is a schematic structural diagram of an organ preservation apparatus according to an embodiment of the present invention;

fig. 2 is a schematic structural view of another organ preservation apparatus according to an embodiment of the present invention;

fig. 3 is a schematic structural view of another organ preservation apparatus according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention;

fig. 7 is a flowchart of an organ preservation method according to an embodiment of the present invention;

fig. 8 is a flow chart of another organ preservation method according to an embodiment of the present invention;

fig. 9 is a flowchart of another organ preservation method according to an embodiment of the present invention.

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and together with the description, serve to explain the principles of the application.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

Fig. 1 is a schematic view of an organ preservation apparatus according to an embodiment of the present invention, as shown in fig. 1, the organ preservation apparatus includes: the device comprises a transfer tank 01, a recovery tank 02, a liquid supplementing assembly 03, a gas supplementing assembly 04, a gas-liquid mixing assembly 05, a filling assembly 06, a backflow assembly 07 and a box body 08. The recycling tank 02 is used for placing an organ 09, the liquid supplementing assembly 03 is communicated with the recycling tank 01, a gas-liquid input end (temporarily not marked in figure 1) of the gas-liquid mixing assembly 05 is communicated with the gas supplementing assembly 04 and the recycling tank 01, a gas-liquid output end (temporarily not marked in figure 1) of the gas-liquid mixing assembly 05 is communicated with the perfusion assembly 06, the perfusion assembly 06 is also communicated with an artery (not shown in figure 1) of the organ 09, and the backflow assembly 07 is communicated with the recycling tank 02 and the recycling tank 01.

In summary, in the organ preservation apparatus provided by the embodiment of the present invention, the fluid infusion assembly can supply nutrient fluid to the transfer tank, and the air supplement assembly can supply nutrient gas to the gas-liquid input end of the gas-liquid mixing assembly, so that after the liquid in the transfer tank is pumped into the gas-liquid input end of the gas-liquid mixing assembly by the perfusion assembly, gas-liquid of mixed nutrient fluid and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing assembly, and the gas-liquid is perfused into the organ, so that the organ can perform physiological activities; and, the perfusion module perfuses the organ after a period of time, the nutrient solution can be followed the venous drainage of organ, collect organ exhaust nutrient solution at the recovery jar after, the backward flow subassembly can be with the liquid backward flow of recovery jar in to the transfer jar, make the nutrient solution that the perfusion module can last in with the transfer jar pump the gas-liquid mixed group subassembly, and perfuse the gas-liquid of gas-liquid mixed subassembly's gas-liquid output to the organ, make the organ can carry out physiological motion in longer time span, be favorable to improving the time of preserving the organ.

Optionally, the organ preservation apparatus may further comprise a controller (not shown in fig. 1), which may control the operation of the fluid replacement assembly 03 to supply the nutrient solution to the transfer tank 01; the controller can also control the gas supplementing assembly 04 to be started so as to supplement nutrient gas to the gas-liquid input end of the gas-liquid mixing assembly 05, and control the perfusion assembly 06 to be started so as to pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing assembly 05 and perfuse the gas and liquid output by the gas-liquid output end of the gas-liquid mixing assembly 05 into the artery of the organ 09; the controller may also control the reflux assembly 07 to activate to reflux the liquid in the recovery tank 02 to the transfer tank 01.

It should be noted that, the gas-liquid input end and the gas-liquid output end of the gas-liquid mixing component are communicated, after the gas supplementing component supplies nutrient gas to the gas-liquid input end of the gas-liquid mixing component, because the perfusion component is communicated with the gas-liquid output end of the gas-liquid mixing component, after the perfusion component is started, the gas-liquid output end of the gas-liquid mixing component can generate negative pressure, so that the nutrient solution in the transfer tank is pumped into the input end of the gas-liquid mixing component, so that the nutrient solution and the nutrient gas are mixed in the gas-liquid mixing component (namely, the nutrient gas is dissolved in the nutrient solution), and the nutrient solution is output from the output end of the gas-liquid mixing component, so that the perfusion.

Fig. 2 is a schematic view of another organ preservation apparatus according to an embodiment of the present invention, as shown in fig. 2, the organ preservation apparatus may further include: the liquid level monitoring assembly 10 is fixed on the side wall of the transit tank 01.

Alternatively, the controller may control the level monitoring assembly 10 to monitor the level of the liquid in the transfer tank 01.

The controller can control the air replenishing component 04 and the filling component 06 to start after the liquid level of the liquid in the transfer tank 01 reaches the transfer low level S2. Thus, the situation that the filling assembly 06 cannot pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing assembly 05 due to the fact that the liquid level of the liquid in the transfer tank 01 is too low can be avoided.

It should be noted that the filling rate of the filling assembly 06 is smaller than the fluid replacement rate of the fluid replacement assembly 03.

The controller may control the fluid replacement assembly 03 to pause when the level of the fluid in the transfer tank 01 reaches the transfer high level S1. Therefore, the liquid in the transfer tank 01 can be prevented from overflowing in the process of replenishing the liquid replenishing assembly 03 into the transfer tank 01.

The controller may also adjust the rate at which the return assembly 07 returns the liquid in the recovery tank 02 to the transfer tank 01 such that the level of the liquid in the transfer tank 01 is below the intermediate high level S1 and above the intermediate low level S2 when the level of the liquid in the transfer tank 01 reaches the intermediate high level S1 or below the intermediate low level S2 during operation of the return assembly 07.

Illustratively, during operation of the backflow assembly 07, when the level of the liquid in the transfer tank 01 reaches the transfer high level S1, the controller may control the backflow assembly 07 to decrease the rate of the liquid in the recovery tank 02 flowing back to the transfer tank 01, so that the level of the liquid in the transfer tank 01 gradually decreases until the level of the liquid in the transfer tank 01 is lower than the transfer high level S1; when the liquid level in the transfer tank 01 is lower than the transfer low level S2, the controller may increase the rate at which the backflow module 07 returns the liquid in the recovery tank 02 to the transfer tank 01, so that the liquid level in the transfer tank 01 gradually increases until the liquid level in the transfer tank 01 is higher than the transfer low level S2.

So, can avoid overflowing because of the liquid level of liquid is too high in the transfer jar 01 simultaneously to and can avoid leading to pouring into the subassembly 06 can't be with the liquid pump income gas-liquid mixture subassembly 05 in the transfer jar 01 because of the liquid level of liquid is too low in the transfer jar 01, further promoted the continuation of supplying with the nutrient solution to gas-liquid mixture subassembly 05, thereby further promoted the continuation that pours subassembly 06 and pours organ 09, be favorable to further improving the length of time of preserving the organ.

Alternatively, the transit liquid level monitoring assembly 10 may include a transit high level monitoring element 101 and a transit low level monitoring element 102, wherein the transit high level monitoring element 101 is attached to a transit high level of the sidewall of the transit tank 01, and the transit low level monitoring element 102 is attached to a transit low level of the sidewall of the transit tank 01. The controller can control the transit high-level monitoring element 101 and the transit low-level monitoring element 102 to monitor whether liquid exists at the respective positions, when the transit high-level monitoring element 101 and the transit low-level monitoring element 102 monitor that the liquid exists at the respective positions, the liquid level of the liquid in the transit tank 01 reaches the transit high level S1, when the transit high-level monitoring element 101 and the transit low-level monitoring element 102 monitor that the respective positions do not have the liquid, the liquid level of the liquid in the transit tank 01 is lower than the transit low level S2, and when the transit high-level monitoring element 101 monitors that the positions do not have the liquid and the low-level monitoring element 112 monitors that the positions do have the liquid, the liquid level of the liquid in the transit tank 01 is between the transit high level S1 and the transit low level S2.

Alternatively, the middle high level monitoring element 101 and the middle low level monitoring element 102 may be external liquid level switches (e.g., capacitive liquid level switches using the sensing capacitance to determine whether the liquid level point is reached or not, or ultrasonic liquid level switches using the echo signal strength to determine whether the liquid level point is reached or not).

Fig. 3 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention, as shown in fig. 3, the organ preservation apparatus may further include: nutrient solution monitoring components 11, recovery liquid level monitoring components 12, waste liquid jar 13 and waste liquid discharge assembly 14, wherein, recovery tank 02 internal fixation is provided with screen panel W1, and this screen panel W1 is used for bearing organ 09, and nutrient solution monitoring components 11 is located transfer tank 01, and recovery liquid level monitoring components 12 fixes the lateral wall at recovery tank 02, and waste liquid discharge assembly 14 intercommunication recovery tank 02 and waste liquid jar 13.

Alternatively, the controller may control the recovery level monitoring assembly 12 to monitor the level of liquid in the recovery tank 02.

During operation of the reflux assembly 07, when the level of the liquid in the recovery tank 02 reaches the recovery high level S3, the controller may adjust the rate at which the reflux assembly 07 refluxes the liquid in the recovery tank 02 to the transfer tank 01 until the level of the liquid in the transfer tank 01 is below the recovery high level S3.

Thus, overflow due to an excessively high liquid level in the recovery tank 02 can be avoided. Need explain, the controller is according to retrieving the specific process of the liquid backward flow of jar 02 in with the recovery jar of the liquid reflux subassembly 07 in the jar 02 to the speed of transfer jar 01 in the jar 02, can refer to the process of the liquid backward flow of jar 02 in will retrieving the liquid of jar 02 to the speed of transfer jar 01 according to the liquid reflux subassembly 07 in the transfer jar 01 of above-mentioned controller, the embodiment of the utility model is not repeated here.

The controller can also control the nutrient solution monitoring component 11 to monitor the concentration of the given nutrient substance in the liquid in the transfer tank 01, and control the backflow component 07 to pause and the waste liquid discharge component 14 to start when the concentration of the given nutrient substance in the liquid in the transfer tank 01 is lower than the given concentration so as to discharge the liquid in the recovery tank 01 into the waste liquid tank 13.

It should be noted that, when the concentration of the given nutrient in the liquid in the relay tank 01 is lower than the given concentration, it is indicated that the given nutrient in the liquid circulating between the relay tank 01 and the recovery tank 02 is about to be consumed by the organ 09, the given nutrient in the liquid in the relay tank 01 is to be replenished, and in order to facilitate the subsequent replenishment of a new nutrient solution in the relay tank 01, the liquid in the recovery tank 02 may be first drained into the waste liquid tank 13.

The controller may also control the waste liquid discharge assembly 14 to be suspended when the liquid level of the liquid in the relay tank 01 is lower than the relay low level S2 and the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4 during the operation of the waste liquid discharge assembly 14. In the height direction of the recovery tank 02, the height of the recovery high position S3 and the height of the recovery low position S4 are both lower than the height of the mesh enclosure W1.

In addition, during the operation of the waste liquid discharge assembly 14, since the operation of the perfusion assembly 06 is not stopped temporarily, the liquid in the transfer tank 01 is consumed continuously until the liquid level of the liquid in the transfer tank 01 is lowered to be lower than the transfer low level S2, and when the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2 and the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4, it is indicated that the liquid in the transfer tank 01 and the liquid in the recovery tank 02 are substantially drained, so that a new nutrient solution can be supplied to the transfer tank 01 in the next step.

The controller can control the liquid supplementing assembly 03 to start, and control the liquid supplementing assembly 03 to pause and control the backflow assembly 07 to start when the liquid level of the liquid in the transfer tank 01 reaches the transfer high level S1.

Thus, most of the liquid in the relay tank 01 and the recovery tank 02 can be drained in time before the given nutrient in the liquid circulating between the relay tank 01 and the recovery tank 02 is almost consumed by the organ 09, and new nutrient solution can be replenished to the relay tank 01 in time, so that sufficient nutrient can be continuously supplied to the organ 01, and the duration of preserving the organ 09 can be further prolonged.

Alternatively, the recovery liquid level monitoring assembly 12 may include a recovery high level monitoring element 121 and a recovery low level monitoring element 122, the recovery high level monitoring element 121 is attached to the recovery high level of the sidewall of the recovery tank 02, and the recovery low level monitoring element 122 is attached to the recovery low level of the sidewall of the recovery tank 02. It should be noted that, the specific process of the high-low level is retrieved in the monitoring of controller control recovery high-level monitoring element 121 and recovery low-level monitoring element 122 can refer to the monitoring process of above-mentioned controller control transfer high-level monitoring element 101 and transfer low-level monitoring element 102, the embodiment of the utility model is not repeated herein.

Alternatively, the given nutrient may be glucose, and the given concentration may be any concentration ranging between 3.9 millimoles per liter and 6.1 millimoles per liter, such as 3.9 millimoles per liter, 4.5 millimoles per liter, or 5 millimoles per liter, and so forth. Alternatively, the given substance may be potassium ion and the given concentration may be any concentration ranging between 3.5 millimoles per liter and 5.5 millimoles per liter, such as 3.5 millimoles per liter, 4.0 millimoles per liter, or 4.5 millimoles per liter, and so on. Alternatively, the given substance may also be sodium ions, and the given concentration may be any concentration ranging between 135 millimoles per liter and 155 millimoles per liter, such as 135 millimoles per liter, 140 millimoles per liter, or 145 millimoles per liter, and so forth.

Optionally, the nutrient monitoring assembly 11 may include: at least one given nutrient monitoring electrode capable of monitoring a given nutrient concentration, the at least one nutrient monitoring electrode may comprise: glucose monitoring electrode, potassium ion monitoring electrode and sodium ion monitoring electrode.

Optionally, the nutrient gas may include oxygen.

Fig. 4 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention, as shown in fig. 4, the organ preservation apparatus further includes: the liquid-gas analyzer 15 is communicated with the gas-liquid output end of the gas-liquid mixing component 05, and the liquid-gas analyzer 15 is communicated with the gas-liquid output end of the gas-liquid mixing component 05.

Optionally, the controller may also control the gas-liquid input end of the gas supplementing assembly 04 gas-liquid mixing assembly 05 to supplement oxygen during the operation of the gas supplementing assembly 04.

The controller may also control the liquid-gas analyzer 15 to monitor the oxygen partial pressure of the gas and liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05.

When the oxygen partial pressure of the gas and the liquid output by the gas-liquid output end of the gas-liquid mixing component 05 exceeds a given oxygen partial pressure range, the rate of supplying the oxygen gas to the gas-liquid input end of the gas-liquid mixing component 05 or the concentration of the supplied oxygen gas by the gas supply component 04 is adjusted until the gas-liquid oxygen partial pressure output by the gas-liquid output end of the gas-liquid mixing component 05 is restored to be within the given oxygen partial pressure range.

For example, when the oxygen partial pressure of the gas and liquid output from the gas and liquid output end of the gas and liquid mixing module 05 is higher than the given oxygen partial pressure range, the controller may decrease the oxygen supply rate of the gas supply module 04 to the gas and liquid mixing module 05 or decrease the concentration of the supplied oxygen, so as to decrease the oxygen partial pressure of the gas and liquid output from the gas and liquid output end of the gas and liquid mixing module 05 until the oxygen partial pressure of the gas and liquid is restored to the given oxygen partial pressure range; when the oxygen partial pressure of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component 05 is lower than the given oxygen partial pressure range, the controller can increase the oxygen supply rate of the gas supply component 04 to the gas and liquid mixing component 05 or increase the concentration of the supplied oxygen, so that the oxygen partial pressure of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component 05 is increased until the oxygen partial pressure of the gas and liquid is restored to the given oxygen partial pressure range.

Thus, the gas-liquid output by the gas-liquid output end of the gas-liquid mixing component 05 can be always kept in or near the given oxygen partial pressure range, so that the organ 09 is prevented from oxygen deficiency due to too low oxygen partial pressure of the gas-liquid filled into the organ 09 or organ oxygen poisoning due to too high oxygen partial pressure of the gas-liquid filled into the organ 09 is avoided, and the duration of organ preservation is further prolonged.

The oxygen partial pressure of the liquid in which oxygen is dissolved refers to the pressure generated by the oxygen dissolved in the liquid, and the oxygen partial pressure can reflect the oxygen carrying capacity of the liquid, that is, the higher the oxygen partial pressure is, the stronger the oxygen carrying capacity of the liquid is, and the lower the oxygen partial pressure is, the weaker the oxygen carrying capacity of the liquid is.

Alternatively, the given oxygen partial pressure may range from 300 mm mercury to 500 mm mercury. The millimeter mercury column is a millimeter mercury column, and means a unit of the pressure value directly expressed by a millimeter number of the height of the mercury column.

Optionally, the nutrient gas may also include carbon dioxide.

With continued reference to fig. 4, during the operation of the gas make-up assembly 04, the controller may control the gas make-up assembly 04 to make up carbon dioxide to the input end of the gas-liquid mixing assembly 05.

The controller can control the analysis of the gas-liquid 16 to monitor the pH value of the gas-liquid output by the gas-liquid output end of the gas-liquid mixing component 05.

When the ph value of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component 05 exceeds a given ph value range, the controller can adjust the rate of supplying carbon dioxide to the gas and liquid input end of the gas and liquid mixing component 05 by the gas supply component 04 until the ph value of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component is restored to be within the given ph value range.

For example, when the ph of the gas-liquid output end of the gas-liquid mixing assembly 05 is higher than the given ph range, the controller may increase the rate at which the gas supplementing assembly 04 supplements carbon dioxide to the gas-liquid input end of the gas-liquid mixing assembly 05, so as to gradually increase the concentration of the carbon dioxide dissolved in the nutrient solution, so as to gradually increase the acidity of the nutrient solution (i.e., the gas-liquid output end of the gas-liquid mixing assembly 05) in which the carbon dioxide is dissolved, and gradually decrease the ph until the ph of the gas-liquid returns to the given ph range. When the ph value of the nutrient solution dissolved with carbon dioxide is lower than a given ph value range, the controller can reduce the rate of supplying carbon dioxide to the gas-liquid input end of the gas-liquid mixing assembly 05 by the supplying assembly 05, so that the concentration of the carbon dioxide dissolved in the nutrient solution is gradually reduced, the alkalinity of the nutrient solution dissolved with carbon dioxide (namely, the gas and liquid output by the gas-liquid output end of the gas-liquid mixing assembly 05) is gradually increased, and the ph value gradually rises until the ph value of the gas-liquid is restored to the given ph value range.

It should be noted that, in the embodiment of the present invention, the pH of the gas-liquid refers to the hydrogen ion concentration index (i.e., pH value), and the higher the pH is, the stronger the alkalinity is, the weaker the acidity is, and the lower the pH is, the stronger the acidity is, and the weaker the alkalinity is.

It should be noted that, in the process that the perfusion module perfuses the gas and liquid output by the gas-liquid output end of the gas-liquid mixing module 05 into the artery of the organ 09, part of the gas and liquid is brought into the gas-liquid analyzer 15, so that the subsequent gas-liquid analyzer can analyze the oxygen partial pressure and the ph value of the gas and liquid.

Alternatively, the controller may control the liquid-gas analyzer to analyze the oxygen partial pressure and the ph of the gas at intervals of a given time period in the process of controlling the liquid-gas analyzer to monitor the oxygen partial pressure and the ph of the gas. For example, the given time period may be any time period ranging from 30 minutes to 40 minutes, such as 30 minutes, 33 minutes, 35 minutes, 37 minutes, or the like.

Fig. 5 is a schematic structural diagram of another organ preservation apparatus according to an embodiment of the present invention, and as shown in fig. 5, the box body may include: the device comprises an outer box 081, an inner box 082 and a heating element 083, wherein the outer box 081 wraps the inner box 082, the heating element 083 is positioned between the outer box 081 and the inner box 082, a water bath 16 and a temperature measuring element 17 are arranged in the inner box 082, and a transfer tank 01 and a recovery tank 02 are both placed in the water bath 16.

Alternatively, the controller may control the temperature measuring element 17 to monitor the temperature in the water bath 16, and after the temperature in the water bath 16 exceeds a given temperature range, adjust the heating power of the heating member 083 to restore the temperature in the water bath 16 to the given temperature range.

For example, when the temperature measuring element 17 detects that the temperature in the water bath 16 is higher than the given temperature range, the controller may decrease the heating power of the heating element 083 to gradually decrease the temperature in the water bath 16 until the temperature in the water bath 16 returns to the given temperature range; when the temperature measuring element 17 detects that the temperature in the water bath 16 is lower than the given temperature range, the controller may increase the heating power of the heating member 083 to gradually increase the temperature in the water bath 16 until the temperature in the water bath 16 returns to the given temperature range.

With such a structure, on one hand, because the water bath 16 is filled with a liquid medium, the transfer tank 01 and the recovery tank 02 are in contact with the liquid medium, and because the liquidity of the liquid medium is strong, the transfer tank 01 and the recovery tank 02 are heated uniformly, the temperature change processes in the transfer tank 01 and the recovery tank 02 are uniform, the temperature change of the liquid perfused to the organ 09 is uniform, the stability of the physiological environment of the organ 09 is high, and the preservation duration of the organ 09 is further prolonged; on the other hand, the box body is of a double-layer box body structure, and the heating element is positioned between the inner box body and the outer box body, so that the temperature is not easy to dissipate, and the heat preservation effect of the box body is better.

Alternatively, the given temperature range may be between 36.5 degrees celsius and 37.5 degrees celsius.

Optionally, the housing may include a plurality of heating members 083 and the plurality of heating members 083 may be affixed to the walls of the inner housing 082. Illustratively, the housing may include three heating members 083, and the three heating members 083 may be distributed on the bottom wall and the two side walls of the inner housing 082, respectively.

Fig. 6 is a schematic view of another organ preservation apparatus according to an embodiment of the present invention, as shown in fig. 6, the fluid infusion assembly may include: the first peristaltic pump 031, nutrient solution source 032, first suction tube 033 and fluid infusion pipe 034, wherein the first peristaltic pump 031 is connected with the nutrient solution source 032 through the first suction tube 033, and is connected with the transit tank 01 through the fluid infusion pipe 034.

Optionally, the controller may control the first peristaltic pump 031 to be activated to pump the nutrient solution from the nutrient solution source 032 into the transfer tank 01 during the process of controlling the solution feeding assembly to feed the nutrient solution into the transfer tank 01, and may control the first peristaltic pump 031 to pause to control the solution feeding assembly to pause when the level of the liquid in the transfer tank 01 reaches the transfer high level S1.

Alternatively, the nutrient solution in the nutrient solution source 032 can be a cell culture medium, such as 1640/DMEM medium or DMEM medium, and it should be noted that 1640/DMEM medium and DMEM medium are cell culture media which are well-established in the related art.

Optionally, the gas supply assembly may include: the device comprises an air pump 041, a first flow valve 042, a second flow valve 043, a third flow valve 044, a first air suction pipe 045, a second air suction pipe 046, a third air suction pipe 047, an air inlet pipe 048, an air supplementing pipe 049, an oxygen source N1 and a carbon dioxide source N2. The air pump 041 is communicated with the first flow valve 042, the second flow valve 043 and the third flow valve 053 through an air inlet pipe 048 and is communicated with an air-liquid input end of the air-liquid mixing component 05 through an air supplementing pipe 058; the first flow valve 042 is communicated with an oxygen source N1 through a first air exhaust pipe 045, and the second flow valve 043 is communicated with the outside air through a second air exhaust pipe 046; the third flow valve 044 is connected to a carbon dioxide source N2 through a third extraction tube 047.

The oxygen source N1 stores high-concentration oxygen, which is much higher than the oxygen in the air; the carbon dioxide source N2 stores high concentration carbon dioxide having a concentration much higher than that of carbon dioxide in the air.

The controller may control the first flow valve 042 and/or the second flow valve 043 to open and control the air pump 041 to pump oxygen from the oxygen source N1 and/or the outside air to the gas-liquid input end of the gas-liquid mixing module 05 in the process of controlling the gas supplementing module to supplement oxygen to the gas-liquid input end of the gas-liquid mixing module 05.

Additionally, the controller may adjust the rate of oxygen supply to the gas-liquid input of the gas-liquid mixing assembly 05 by adjusting the flow rate of the first flow valve 042 and/or the second flow valve 043. The controller may adjust a flow rate difference between the flow rate of the first flow valve 042 and the flow rate of the second flow valve 043 to adjust the concentration of the oxygen gas supplied to the gas-liquid input end of the gas-liquid mixing assembly 05; for example, the controller may increase the flow rate of the first flow valve 042 and decrease the flow rate of the second flow valve 043 to increase the concentration of the oxygen supplied from the air pump 041 to the air-liquid mixing assembly 05, and may decrease the flow rate of the first flow valve 042 and increase the flow rate of the second flow valve 043 to decrease the concentration of the oxygen supplied from the air pump 041 to the air-liquid mixing assembly 05.

The controller may control the third flow valve 044 to open and control the air pump 041 to pump the carbon dioxide in the carbon dioxide source X2 to the gas-liquid input end of the gas-liquid mixing assembly 05 in the process of controlling the gas supplementing assembly to supplement the carbon dioxide to the gas-liquid input end of the gas-liquid mixing assembly 05. Additionally, the controller may also regulate the rate of carbon dioxide supply to the gas-liquid input of the gas-liquid mixing assembly 05 by regulating the flow rate of the third flow valve 044.

Optionally, the organ preservation apparatus may further comprise: the liquid conveying pipe 18 is arranged, and the gas-liquid input end of the gas-liquid mixing component 05 is communicated with the transfer tank 01 through the liquid conveying pipe 18.

Optionally, the perfusion assembly may comprise: the second peristaltic pump 061, the second liquid pumping pipe 062 and the perfusion pipe 063, wherein the second peristaltic pump 061 is communicated with the gas-liquid output end of the gas-liquid mixing component 05 through the second liquid pumping pipe 062, and the second peristaltic pump 061 is communicated with the artery of the organ 09 through the perfusion pipe 063.

The controller can control the second peristaltic pump 061 to pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing component 05, and the liquid output by the gas-liquid output end of the gas-liquid mixing component 05 is poured into the artery of the organ 09.

Optionally, the reflow assembly may include: a third peristaltic pump 071, a third liquid pumping pipe 072 and a liquid return pipe 073, wherein the third peristaltic pump 071 can be communicated with the recovery tank 02 through the third liquid pumping pipe 072 and communicated with the transfer tank 01 through the liquid return pipe 073.

Alternatively, the third liquid extracting pipe 072 is communicated with the recovery tank 02 at the bottom of the recovery tank 02, and the liquid returning pipe 073 is communicated with the transfer tank 01 at the bottom of the transfer tank 01.

The controller may control the third peristaltic pump 071 to pump the liquid collected in the recovery tank 02 into the transfer tank 01 during the operation of the reflux assembly, may control the third peristaltic pump 071 to pump the liquid collected in the recovery tank 02 into the transfer tank 01 by adjusting the rate at which the third peristaltic pump 071 pumps the liquid collected in the recovery tank 02 into the transfer tank 01 when the liquid level of the liquid in the transfer tank 01 reaches the transfer high position S1 or is lower than the transfer low position S2, so that the liquid level of the liquid in the transfer tank 01 is lower than the transfer high position S1 and higher than the transfer low position S2, and may control the third peristaltic pump 071 to pause when the nutrient monitoring assembly 11 monitors that the concentration of a given nutrient in the liquid in the transfer tank 01 is lower than a given concentration.

Alternatively, the waste liquid discharge assembly may include: a fourth peristaltic pump 141, a fourth liquid drawing pipe 142 and a liquid discharge pipe 143, wherein the fourth peristaltic pump 141 is communicated with the recovery tank 02 through the fourth liquid drawing pipe 142 and is communicated with the waste liquid tank 13 through the liquid discharge pipe 143.

Optionally, a fourth extractor tube 142 communicates with the recovery tank 02 at the bottom of the recovery tank 02.

The controller may control the fourth peristaltic pump 141 to discharge the liquid in the recovery tank 02 into the waste liquid tank 13 in the course of controlling the operation of the waste liquid discharge assembly, and control the fourth peristaltic pump 141 to be suspended when the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2 and the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4.

Optionally, the organ preservation apparatus may further comprise: the liquid outlet pipe 19, and the gas-liquid output end of the gas-liquid mixing component 05 can be communicated with the gas-liquid analyzer 15 through the liquid outlet pipe 19.

Optionally, the organ preservation apparatus may further comprise a buzzer (not shown in fig. 6). During the operation of the filling assembly, when the liquid level of the liquid in the transfer tank 01 is higher than the transfer high level S1 or lower than the transfer low level S2, when the liquid level of the liquid in the recovery tank 02 is higher than the recovery high level S3 or lower than the recovery low level S4, or when the temperature in the water bath 16 exceeds a given temperature range, the controller can control the buzzer to alarm to remind the staff and avoid accidents.

Alternatively, the gas-liquid mixing assembly 05 may be a membrane lung. It should be noted that membrane lung is also called as extracorporeal membrane oxygenation, which can function as an artificial heart lung and can dissolve nutrient gases such as oxygen and carbon dioxide into nutrient solution.

Alternatively, in the height direction of the transfer pot 01, the distance from the transfer high position S1 to the opening of the transfer pot 02 and the distance from the transfer low position S2 to the opening of the infusion tube 18 may be both the first given distance, and the height of the transfer high position S1 is lower than the opening of the transfer pot 01 and the height of the transfer low position S2 is higher than the opening of the infusion tube 18. Alternatively, the first given distance may be any length between 3 mm and 10 mm, for example: 3 mm, 5 mm, 7 mm, 9 mm or 10 mm.

Alternatively, in the height direction of the recovery tank 02, the distance from the recovery high position S3 to the mesh enclosure W1 and the distance from the recovery low position S4 to the fourth liquid pumping pipe 142 may be a second given distance, and the height of the recovery low position S4 is higher than the nozzle of the fourth liquid pumping pipe 142. Alternatively, the second given distance may be any length between 3 mm and 10 mm, for example: 3 mm, 5 mm, 7 mm, 9 mm or 10 mm.

It should be noted that, in fig. 1 to fig. 6, only each component in the organ preservation apparatus is taken as an example and is independent from the box, and optionally, each component may also be integrated on the box, for example, the peristaltic pump in the air supply component, the fluid infusion component, the perfusion component and the waste fluid discharge component may be integrated on the inner wall of the box, and the pipe connected to the peristaltic pump may extend into the inner wall of the box, which is not limited by the embodiment of the present invention.

Alternatively, organ 09 may be an internal organ in the human body, such as the liver, spleen, lung, kidney, heart, or small intestine.

It should be noted that, in the embodiment of the present invention, the controller of the organ preservation apparatus can be connected to and communicate with each component (each peristaltic pump, each flow valve, air pump, each liquid level monitoring element, each heating element, temperature measuring element, liquid-gas analyzer, nutrient solution monitoring component) which needs to be controlled by the controller through a signal connection line. Alternatively, the controller may also perform communication (e.g., wireless communication) in other manners, which is not limited in the embodiment of the present invention.

Fig. 7 is a flow chart of an organ preservation method according to an embodiment of the present invention, which may be used in a controller of any of the above-mentioned organ preservation apparatuses, and the organ preservation method may include:

and 701, controlling the liquid supplementing assembly to start so as to supplement nutrient solution to the transfer tank.

And step 702, controlling the gas supplementing assembly to start so as to supplement nutrient gas to the gas-liquid input end of the gas-liquid mixing assembly.

And 703, controlling the start of the perfusion assembly to pump the liquid in the transfer tank into a gas-liquid input end of the gas-liquid mixing assembly and perfuse the gas-liquid output by a gas-liquid output end of the gas-liquid mixing assembly into an artery of the organ.

And 704, controlling the backflow component to start so as to enable the liquid in the recovery tank to flow back to the transfer tank.

In summary, in the organ preservation method provided by the embodiment of the present invention, after the control solution infusion assembly supplies the nutrient solution to the transfer tank, the control solution infusion assembly supplies the nutrient gas to the gas-liquid input end of the gas-liquid mixing assembly, so that after the perfusion assembly pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing assembly, the gas-liquid output end of the gas-liquid mixing assembly generates the gas-liquid mixture of the nutrient solution and the nutrient gas, and perfuses the gas-liquid mixture into the organ, so that the organ can perform physiological activities; and, after control perfusion assembly perfused the organ a period, nutrient solution can be followed the venous drainage of organ, collect organ exhaust nutrient solution at the recovery jar after, the controller control backward flow subassembly is with the liquid backward flow of recovery jar in to the transfer jar, make that perfusion assembly can last with the nutrient solution in the transfer jar pump gas-liquid mixed group subassembly, and perfuse the gas-liquid of gas-liquid mixed subassembly's gas-liquid output to the organ, make the organ can carry out physiological activity in longer time span, be favorable to improving the time of preserving the organ.

Fig. 8 is a flow chart of another organ preservation method according to an embodiment of the present invention, which can be used for the controller of the above-mentioned organ preservation apparatus including the transit liquid level monitoring assembly, and the organ preservation method can include:

step 801, controlling the liquid supplementing assembly to start so as to supplement nutrient solution to the transfer tank.

And step 802, controlling the transfer liquid level monitoring assembly to monitor the liquid level of the liquid in the transfer tank.

And 803, controlling the air supplement component and the filling component to start after the liquid level of the liquid in the transfer tank reaches the transfer low level.

It should be noted that the filling rate of the filling assembly is smaller than the fluid replacement rate of the fluid replacement assembly.

And step 804, controlling the liquid supplementing assembly to pause when the liquid level of the liquid in the transfer tank reaches a transfer high level.

And 805, controlling the backflow component to start so as to enable the liquid in the recovery tank to flow back to the transfer tank.

And 806, when the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjusting the speed of the backflow component for enabling the liquid in the recovery tank to flow back to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the transfer high level and higher than the transfer low level.

Fig. 9 is a flowchart of another organ preservation method according to an embodiment of the present invention, in which the organ preservation method can be applied to the above-mentioned controller including the nutrient solution monitoring component, the recovery liquid level monitoring component, the waste liquid tank, and the waste liquid discharging component, and the recovery tank is fixedly disposed in the organ preservation device with a mesh enclosure, and the organ preservation method can include:

and step 901, controlling the liquid supplementing assembly to start so as to supplement nutrient solution to the transfer tank.

And step 902, controlling the transfer liquid level monitoring assembly to monitor the liquid level of the liquid in the transfer tank.

And step 903, controlling the air replenishing assembly and the filling assembly to start after the liquid level of the liquid in the transfer tank reaches a transfer low level.

And 904, controlling the liquid supplementing assembly to pause when the liquid level of the liquid in the transfer tank reaches a transfer high level.

And 905, controlling the backflow component to start so as to enable the liquid in the recovery tank to flow back to the transfer tank.

And 906, when the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjusting the speed of the backflow component for enabling the liquid in the recovery tank to flow back to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the transfer high level and higher than the transfer low level.

Step 907, controlling the recovery liquid level monitoring assembly to monitor the liquid level of the liquid in the recovery tank.

And 908, when the liquid level of the liquid in the recovery tank reaches the recovery high level, adjusting the speed of the backflow component for enabling the liquid in the recovery tank to flow back to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the recovery high level.

Step 909, control the nutrient monitoring component to monitor the concentration of a given nutrient in the liquid in the transfer pot.

And 910, when the concentration of the given nutrient substances in the liquid in the transfer tank is lower than the given concentration, controlling the backflow component to pause, and controlling the waste liquid discharge component to start so as to discharge the liquid in the recovery tank into a waste liquid tank.

And 911, controlling the waste liquid discharge assembly to pause when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level.

It should be noted that the height of the position of the recovery high position and the height of the position of the recovery low position are both lower than the height of the position of the mesh enclosure.

And 912, controlling the liquid supplementing assembly to start, and controlling the liquid supplementing assembly to pause and the backflow assembly to start when the liquid level of the liquid in the transfer tank reaches a transfer high level.

Alternatively, in any of the

above steps

703, 803 and 903, the controller in the organ preservation apparatus may control the gas supply module to supply oxygen to the gas-liquid input end of the gas-liquid mixing module.

Optionally, in any organ preservation method above, the controller may control the liquid-gas analyzer to monitor an oxygen partial pressure of gas and liquid output by a gas-liquid output end of the gas-liquid mixing assembly; when the oxygen partial pressure of the gas and the liquid output by the gas-liquid output end of the gas-liquid mixing component exceeds a given oxygen partial pressure range, adjusting the rate of supplying oxygen or the concentration of the supplied oxygen to the gas-liquid input end of the gas-liquid mixing component by the gas supply component until the oxygen partial pressure of the gas and the liquid output by the gas-liquid output end of the gas-liquid mixing component is restored to be within the given oxygen partial pressure range.

Alternatively, in any of the

above steps

703, 803 and 903, the controller in the organ preservation apparatus may control the gas supply module to supply carbon dioxide to the gas-liquid input end of the gas-liquid mixing module.

Optionally, in any organ preservation method, the controller may control the liquid-gas analyzer to monitor a ph value of the gas-liquid output by the gas-liquid output end of the gas-liquid mixing assembly; when the pH value of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component exceeds a given pH value range, the rate of supplying carbon dioxide to the gas and liquid input end of the gas and liquid mixing component by the gas supplementing component is adjusted until the pH value of the gas and liquid output by the gas and liquid output end of the gas and liquid mixing component is restored to be within the given pH value range.

Alternatively, the controller may control the liquid-gas analyzer to analyze the oxygen partial pressure and the ph of the gas at intervals of a given time period in the process of controlling the liquid-gas analyzer to monitor the oxygen partial pressure and the ph of the gas.

Optionally, in any of the above-mentioned organ preservation methods, when the organ preservation apparatus includes a water bath, a temperature measuring element, and a heating element, the organ preservation method may further include: and controlling the temperature measuring element to monitor the temperature in the water bath, and adjusting the heating power of the heating element after the temperature in the water bath exceeds a given temperature range so as to restore the temperature in the water bath to the given temperature range.

Optionally, when the fluid replacement assembly in the organ preservation apparatus comprises a first peristaltic pump, a nutrient solution source, a first suction tube and a fluid replacement tube, in any one of the

steps

701, 801, 901 and 912, the controller may control the first peristaltic pump to be activated to pump nutrient solution from the nutrient solution source into the organ preservation apparatus; in step 804 or step 904, the controller may control the first peristaltic pump to pause when the level of liquid in the transfer tank reaches the transfer high level.

Optionally, when the air supply assembly in the organ preservation apparatus includes an air pump, a first flow valve, a second flow valve, a third flow valve, a first air pumping pipe, a second air pumping pipe, a third air pumping pipe, an air inlet pipe, an air supplement pipe, an oxygen source, and a carbon dioxide source, in any one of the

above steps

702, 803, and 903, the controller may control the first flow valve and/or the second flow valve to open, and control the air pump to pump the outside air and/or the oxygen in the oxygen source to the air-liquid input end of the air-liquid mixing assembly; in addition, the controller can control the third flow valve to open and control the air pump to pump carbon dioxide in the carbon dioxide source to the gas-liquid input end of the gas-liquid mixing assembly. In addition, the controller may adjust a rate of supplying air to the gas-liquid input of the gas-liquid mixing assembly by adjusting a flow rate of the first flow valve and/or a flow rate of the second flow valve, and may adjust a rate of supplying carbon dioxide to the gas-liquid input of the gas-liquid mixing assembly by adjusting a flow rate of the third flow valve.

Optionally, the perfusion assembly in the organ preservation apparatus comprises: when the second peristaltic pump, the second liquid pumping tube and the perfusion tube are used, in any one of the

steps

703, 803 and 903, the controller may control the second peristaltic pump to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing assembly.

Alternatively, when the reflux assembly in the organ preservation apparatus includes a third peristaltic pump, a third aspiration tube, and a reflux tube, the controller may control the third peristaltic pump to pump the fluid collected in the recovery tank into the transfer tank in any of the

above steps

704, 805, and 905. In step 806 or step 908, the controller may pump the collected liquid from the recycling into the transfer tank by adjusting the rate at which the third peristaltic pump pumps the collected liquid into the transfer tank such that the liquid level in the transfer tank is below the high level of the transfer tank and above the low level of the transfer tank. In step 910, the third peristaltic pump is controlled to pause when the nutrient monitoring assembly detects that the concentration of the given nutrient in the liquid in the transfer tank is lower than the given concentration.

Alternatively, when the waste liquid discharging assembly in the organ preservation apparatus comprises a fourth peristaltic pump, a fourth liquid suction pipe and a liquid discharge pipe, in step 910, the controller may control the fourth peristaltic pump to discharge the liquid in the recovery tank into the waste liquid tank; in the step 911, when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the fourth peristaltic pump is controlled to pause.

It should be noted that the embodiment of the present invention provides an apparatus embodiment, which can be mutually referred to with a corresponding method embodiment, and the embodiment of the present invention does not limit this. The embodiment of the utility model provides a proper adjustment can be carried out to the precedence of method embodiment step, and the step also can carry out corresponding increase and decrease according to the condition, and any technical personnel who is familiar with this technical field are in the utility model discloses a within the technical scope, can think about the method that changes easily, all should cover within the protection scope of the utility model, consequently no longer describe repeatedly.

Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.

It will be understood that the invention is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof. The scope of the present invention is limited only by the appended claims.

Claims

1. An organ preservation device, comprising: the device comprises a transfer tank, a recovery tank, a liquid supplementing assembly, a gas-liquid mixing assembly, a filling assembly, a backflow assembly and a box body;

2. The organ preservation apparatus according to claim 1, further comprising: the transfer liquid level monitoring assembly is fixed on the side wall of the transfer tank.

3. The organ preservation apparatus according to claim 2, further comprising: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank and a waste liquid discharging component,

4. The organ preservation apparatus according to any one of claims 1 to 3, further comprising: and the liquid-gas analyzer is communicated with the gas-liquid output end of the gas-liquid mixing component.

5. The organ preservation apparatus according to any one of claims 1 to 3, wherein the case comprises: outer container, inner box and heating member, the outer container parcel the inner box, the heating member is located the outer container with between the inner box, be provided with water bath and temperature element in the inner box, the transit jar with retrieve the jar and all place the water bath is internal.

6. The organ preservation apparatus according to any one of claims 1 to 3, further comprising: the gas-liquid input end of the gas-liquid mixing component is communicated with the transfer tank through the liquid conveying pipe;

7. The organ preservation apparatus according to claim 2 or 3, wherein the transit level monitoring assembly comprises: the transfer high-order monitoring component is attached in the transfer high-order department of the lateral wall of transfer jar, the transfer low-order monitoring component is attached in the transfer low-order department of the lateral wall of transfer jar.

8. The organ preservation apparatus according to claim 3, wherein the fluid level retrieval monitoring assembly comprises: the recovery high-level monitoring element is attached to the recovery high-level position of the side wall of the recovery tank, and the recovery low-level monitoring element is attached to the recovery low-level position of the side wall of the recovery tank;

9. The organ preservation apparatus according to claim 4, further comprising: and the gas-liquid output end of the gas-liquid mixing assembly is communicated with the liquid-gas analyzer through the liquid outlet pipe.

10. The organ preservation apparatus according to claim 6, wherein the third draw tube communicates with the recovery tank at a bottom thereof, and the return tube communicates with the transfer tank at a bottom thereof.