CN213803834U

CN213803834U - Culture perfusion device

Info

Publication number: CN213803834U
Application number: CN202022279308.8U
Authority: CN
Inventors: 王莉娟
Original assignee: Institute of Zoology of CAS
Current assignee: Institute of Zoology of CAS
Priority date: 2020-10-14
Filing date: 2020-10-14
Publication date: 2021-07-27
Anticipated expiration: 2030-10-14

Abstract

The utility model relates to a culture perfusion device, which comprises a perfusion cover capable of replacing a dish cover on a culture dish; the perfusion cover comprises a cover body, a through cavity formed in the center of the cover body, a liquid inlet perfusion tube and a liquid outlet perfusion tube, wherein the liquid inlet perfusion tube and the liquid outlet perfusion tube are arranged on the cover body in a penetrating mode, the upper ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube penetrate out of the top of the cover body, and the lower ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the bottom of the cover body. The utility model discloses well feed liquor perfusion pipe and play liquid perfusion pipe accessible peristaltic pump respectively with the perfusion bottle, the waste liquid bottle is connected, the culture dish that has the perfusion lid can be fixed on the objective table under the microscope, and then can realize the perfusion of culture, trade liquid and operation such as micro-imaging, whole device simple structure, the operation is convenient easy going, the perfusion lid can be designed according to the specification of culture dish, can use with the cooperation of current experimental facilities, compare in current perfusion equipment, the cost is low, easily popularize and apply.

Description

Culture perfusion device

Technical Field

The utility model relates to a culture perfusion device belongs to biological cultivation field.

Background

When the existing cell perfusion equipment is used, cells need to be planted in a special vessel, and the special vessel is matched with matched instrument equipment for use, so that the perfusion of the cells can be completed, the perfusion equipment needs to be purchased in a complete set, the cost of the commercially available complete perfusion equipment is generally higher, and the cost of cell culture perfusion research is greatly increased; in addition, the existing complete perfusion equipment can only be used for cell perfusion, has single function and cannot meet the requirements of cell culture in various aspects.

Disclosure of Invention

To the above problem, the utility model aims at providing a culture perfusion device, the device can use with equipment cooperation such as current culture dish, microscope, realizes the perfusion of culture, trades liquid, operation such as medicine stimulation, has advantages such as simple operation, with low costs, overcomes current perfusion equipment defect.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a culture perfusion device comprises a perfusion cover capable of replacing a dish cover on a culture dish; the perfusion cover comprises a cover body, a through cavity formed in the center of the cover body, a liquid inlet perfusion tube and a liquid outlet perfusion tube, wherein the liquid inlet perfusion tube and the liquid outlet perfusion tube are arranged on the cover body in a penetrating mode, the upper ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube penetrate out of the top of the cover body, and the lower ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the bottom of the cover body.

In some embodiments, the cover body comprises a first annular cover and a second annular cover arranged at the bottom of the outer side wall of the first annular cover, the inner cavity of the first annular cover is the through cavity, and the liquid inlet perfusion tube and the liquid outlet perfusion tube are symmetrically arranged on the first annular cover in a penetrating way around the central axis of the cover body; the upper ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the top of the first annular cover, and the lower ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the bottom of the first annular cover.

In some embodiments, the bottom surface of the lid is formed with a circular groove that fits the upper peripheral edge of the culture dish.

In some embodiments, two screw bodies are symmetrically arranged on the cover body and relative to the central axis of the cover body, and the lower ends of the screw bodies can be in threaded fit on the object stage of the microscope.

In some embodiments, the device further comprises a perfusion bottle and a waste liquid bottle, wherein the liquid inlet perfusion tube is connected with the perfusion bottle through a pump, and the liquid outlet perfusion tube is connected with the waste liquid bottle through a pump.

In some embodiments, at least a part of the pipe section of the liquid inlet perfusion pipe penetrating out of the bottom of the cover body is a semicircular pipe, and the lower end of the liquid outlet perfusion pipe is a flat round port.

In some embodiments, the perfusion cover further comprises a circular insert removably inserted within the through-cavity of the perfusion cover, the circular insert having a 0.5mm glass slide mounted in the center thereof.

The utility model discloses owing to take above technical scheme, it has following advantage: the utility model provides a culture perfusion device, including the perfusion lid that can replace the ware lid on the culture dish, the perfusion is covered and is worn to establish feed liquor perfusion pipe and goes out liquid perfusion pipe, feed liquor perfusion pipe and play liquid perfusion pipe accessible peristaltic pump respectively with the perfusion bottle, the waste liquid bottle is connected, the culture dish that has the perfusion lid can be fixed on the objective table under the microscope, and then can realize the perfusion of culture in microscopic formation of image, operations such as trade liquid, whole device simple structure, the operation is convenient easy, the perfusion lid can design according to the specification of culture dish, can use with current experimental facilities cooperation, compare in current perfusion equipment, the cost is low, easily popularization and application.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic sectional structure of the present invention;

fig. 3 is a schematic structural diagram of the circular insert of the present invention.

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended as limitations on the scope of the invention, but are merely illustrative of the true spirit of the technical solution of the invention.

As shown in fig. 1, the utility model provides a culture perfusion device, which comprises a perfusion cover 1, wherein the perfusion cover 1 can replace a dish cover on a culture dish, so that the culture can be placed in the culture dish for perfusion, liquid change, drug stimulation and other operations; specifically, the perfusion cover 1 includes a cover body 11, a through cavity 12 formed in the center of the cover body 11, a liquid inlet perfusion tube 13 and a liquid outlet perfusion tube 14 penetrating the cover body 11, wherein the upper ends of the liquid inlet perfusion tube 13 and the liquid outlet perfusion tube 14 penetrate the top of the cover body 11, and the lower ends of the liquid inlet perfusion tube 13 and the liquid outlet perfusion tube 14 penetrate the bottom of the cover body 11.

When the utility model is used, the perfusion cover 1 is used for replacing the dish cover on the culture dish, the cover body 11 is arranged at the top of the culture dish, the lower ends of the liquid inlet perfusion tube 13 and the liquid outlet perfusion tube 14 respectively extend into the culture dish, wherein the liquid inlet perfusion tube 13 is connected with a perfusion bottle through a pump (such as a peristaltic pump), and the liquid outlet perfusion tube 14 is connected with a waste liquid bottle through a pump (such as a peristaltic pump), so that the culture in the culture dish can be conveniently perfused or the liquid can be changed; in addition, the culture dish with the perfusion cover 1 can be fixed on the objective table of a microscope, and the through cavity 2 on the perfusion cover 1 is positioned right below the microscope, so that the microscopic imaging is carried out while perfusion is carried out, and the imaging effect is ensured.

In the above embodiment, preferably, as shown in fig. 2, the bottom surface of the lid 11 is formed with a circular groove 15 that fits the upper peripheral edge of the culture dish, and when mounted, the upper peripheral edge of the culture dish is inserted into the circular groove 15, thereby preventing the lid 11 from moving radially on the culture dish.

In the above embodiment, preferably, in order to facilitate fixing the perfusion cover 1 to the stage of the microscope, two screw bodies 16 are provided on the cover body 11 and symmetrically arranged about the central axis thereof, and the lower ends of the two screw bodies 16 are screwed to the stage.

In the above embodiment, as shown in fig. 1, the cover body 11 includes a first annular cover 111 and a second annular cover 112 disposed at the bottom of the outer side wall of the first annular cover 111, the inner cavity of the first annular cover 111 is the through cavity 12, the liquid inlet filling pipe 13 and the liquid outlet filling pipe 14 are symmetrically disposed on the first annular cover 111 about the central axis of the cover body 11, the upper ends of the liquid inlet filling pipe 13 and the liquid outlet filling pipe 14 both extend out of the top of the first annular cover 111, the lower ends thereof both extend out of the bottom of the first annular cover 111, and the two screw bodies 16 are symmetrically disposed on the second annular cover 112 about the central axis of the cover body 11.

In the above embodiment, preferably, the first annular cover 111 and the second annular cover 112 are integrally formed.

In the above embodiment, it is preferable that at least a part of the pipe section of the inlet perfusion pipe 13 penetrating the bottom of the cover 11 is a semicircular pipe as shown in fig. 2, so that air gently flows through the inlet perfusion pipe 13 from the side when the liquid is fed, bubbles generated when the liquid is fed are reduced, a zero-bubble culture medium is formed, and the lower end of the outlet perfusion pipe 14 is a flat round port.

In the above embodiment, preferably, as shown in fig. 3, the perfusion cover 1 further includes a circular insert 17, the circular insert 17 is movably inserted into the through cavity 12 of the perfusion cover 1, and a 0.5mm glass slide 18 is embedded in the center of the circular insert 17, so that on one hand, the perfusion cover 1 is hermetically arranged on the culture dish, which can avoid the contamination of the culture in the culture dish, and on the other hand, the manual medicine adding can be realized without uncovering the perfusion cover, and furthermore, the glass slide 18 has good light transmittance, which does not affect the effect of the microscopic imaging.

The present invention has been described only with reference to the above embodiments, and the structure, arrangement position and connection of the components may be changed. On the basis of the technical scheme of the utility model, the all sides according to the utility model discloses the principle is all not excluded to the improvement that individual part goes on or the transform of equivalence the utility model discloses a protection scope is outside.

Claims

1. A culture perfusion device, characterized by: the perfusion cover comprises a dish cover capable of replacing a culture dish; the perfusion cover comprises a cover body, a through cavity formed in the center of the cover body, a liquid inlet perfusion tube and a liquid outlet perfusion tube, wherein the liquid inlet perfusion tube and the liquid outlet perfusion tube are arranged on the cover body in a penetrating mode, the upper ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube penetrate out of the top of the cover body, and the lower ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the bottom of the cover body.

2. The culture perfusion device of claim 1, wherein: the cover body comprises a first annular cover and a second annular cover arranged at the bottom of the outer side wall of the first annular cover, the inner cavity of the first annular cover is the through cavity, and the liquid inlet perfusion tube and the liquid outlet perfusion tube are symmetrically arranged on the first annular cover in a penetrating manner around the central axis of the cover body; the upper ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the top of the first annular cover, and the lower ends of the liquid inlet perfusion tube and the liquid outlet perfusion tube extend out of the bottom of the first annular cover.

3. Culture perfusion device according to claim 1 or 2, characterized in that: the bottom surface of the cover body is provided with a circular groove matched with the upper periphery of the culture dish.

4. Culture perfusion device according to claim 1 or 2, characterized in that: two screw cylinders are symmetrically arranged on the cover body and around the central axis of the cover body, and the lower ends of the screw cylinders can be in threaded fit with an objective table of a microscope.

5. The culture perfusion device of claim 1, wherein: the liquid inlet perfusion tube is connected with the perfusion bottle through a pump, and the liquid outlet perfusion tube is connected with the waste liquid bottle through a pump.

6. The culture perfusion device of claim 1, wherein: at least part of the pipe section of the liquid inlet perfusion pipe penetrating out of the bottom of the cover body is a semicircular pipe, and the lower end of the liquid outlet perfusion pipe is a flat round port.

7. The culture perfusion device of claim 1, wherein: the perfusion cover also comprises a circular plug-in unit which is movably inserted into the through cavity of the perfusion cover, and a 0.5mm glass slide is embedded in the center of the circular plug-in unit.