CN115812693A - Myocardial tissue perfusion experiment processing device - Google Patents
Myocardial tissue perfusion experiment processing device Download PDFInfo
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- CN115812693A CN115812693A CN202211374412.2A CN202211374412A CN115812693A CN 115812693 A CN115812693 A CN 115812693A CN 202211374412 A CN202211374412 A CN 202211374412A CN 115812693 A CN115812693 A CN 115812693A
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Abstract
The invention belongs to the technical field of in-vitro tissue experiment devices, and particularly discloses a myocardial tissue perfusion experiment processing device which comprises a perfusion device and a pre-positioning device detachably connected with the perfusion device; the filling device comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, and a cavity formed by the outer wall and the inner wall is a heating cavity; the outer wall is provided with a water inlet and a water outlet; the filling device comprises a heating pipe arranged between the inner wall and the outer wall, the heating pipe is of a spiral structure, and the heating pipe is connected with a filling interface; the pre-positioning device comprises a filling branch pipe detachably connected with the filling interface and a filling main pipe detachably connected with the filling branch pipe, one end of the filling main pipe, far away from the filling branch pipe, is connected with a liquid container, and one end of the filling branch pipe, far away from the filling main pipe, is connected with a filling head; the invention provides a myocardial tissue perfusion experiment processing device capable of being accurately inserted into a perfusion head.
Description
Technical Field
The invention belongs to the technical field of isolated tissue experimental devices, and particularly relates to a myocardial tissue perfusion experimental processing device.
Background
The isolated myocardial tissue perfusion experiment system is one system for isolated heart perfusion experiment. During experiment, animal hearts are taken out, connected to a specific prepositioning device, perfused by perfusate, the control of nerves and body fluid is eliminated, ventricular pressure, arterial blood pressure and electrocardiosignals are recorded by matching with special analysis software, various physiological parameters are automatically analyzed, and the device is used for researches such as cardiac function and hemodynamic changes under the condition of pathophysiology and is widely applied to physiological, pathophysiological and pharmacological researches. In the prior art, the perfusion head needs to be accurately inserted into the artery in the experimental operation process, but the prior art is difficult to judge whether the perfusion head is accurately inserted into the artery in advance; and the perfusion head is difficult to fix after being inserted into the artery, needs to be operated by a plurality of people, and has lower efficiency.
Disclosure of Invention
The invention aims to provide a myocardial tissue perfusion experiment processing device which can be accurately inserted into a perfusion head.
Based on the purpose, the invention adopts the following technical scheme:
a myocardial tissue perfusion experiment processing device comprises a perfusion device and a pre-positioning device detachably connected with the perfusion device; the filling device comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, and a cavity formed by the outer wall and the inner wall is a heating cavity; the outer wall is provided with a water inlet and a water outlet which both communicate the heating cavity with the outside of the container; the filling device comprises a heating pipe arranged between the inner wall and the outer wall, the heating pipe is of a spiral structure, and the heating pipe is connected with a filling interface; the pre-positioning device comprises a filling branch pipe detachably connected with the filling interface and a filling main pipe detachably connected with the filling branch pipe, one end of the filling main pipe far away from the filling branch pipe is connected with a liquid container, and one end of the filling branch pipe far away from the filling main pipe is connected with a filling head.
Further, a liquid inlet communicated with the bottom end opening of the heating pipe is arranged on the outer wall; an exhaust port is arranged at the top of the outer wall, an exhaust pipe is connected between the exhaust port and the opening at the top end of the heating pipe, the exhaust port is connected with the top of the exhaust pipe, the outside of the container is communicated with the exhaust pipe, and gas at the top of the exhaust pipe is exhausted; the lower part of the exhaust pipe is connected with a liquid outlet which is arranged on the inner wall; the filling device comprises a steering pipe, and the steering pipe is arranged in the heating container; the steering tube comprises a transition arc section arranged at the bottom of the heating container, and the transition arc section is arranged between the inner wall and the outer wall; the steering pipe also comprises a liquid inlet straight section and a liquid outlet straight section which are respectively connected with the two ends of the transition arc section; the end of the liquid inlet straight section far away from the transition arc section is connected with the liquid outlet, and the end of the liquid outlet straight section far away from the transition arc section is connected with the filling interface. The filling interface is arranged at the opening at the top of the heating container, and the liquid outlet straight section is arranged in the heating container and clings to the inner wall.
Furthermore, the perfusion device also comprises a negative pressure hose which is connected with a negative pressure pump; the upper portion of heating container is provided with the wire protective tube, and wire protective tube one end is located outside the heating container, and the other end sets up on the inner wall and communicates in with the heating container.
Further, the water inlet and the water outlet are connected with a circulating water bath heating device; the liquid inlet is connected with a perfusion liquid conveying device; the heating container, the heating pipe, the steering pipe and the lead protecting pipe are all made of transparent glass materials.
Furthermore, a vein drip cup is arranged on the perfusion main pipe, and the perfusion branch pipe and the perfusion main pipe are hoses.
Furthermore, the perfusion head comprises a connecting pipe connected in the perfusion branch pipe, and the connecting pipe is made of hard materials; one end of the connecting pipe, which is far away from the perfusion branch pipe, is connected with an inserting head, and an included angle between the inserting head and the connecting pipe is an obtuse angle; the diameter of one end of the insertion head connected with the perfusion branch pipe is larger than that of one end of the insertion head far away from the perfusion branch pipe.
Furthermore, the pre-positioning device comprises a culture dish, a support table is arranged in the culture dish, the support table is of a beveled cylindrical structure coaxial with the culture dish, and the highest point of the support table is arranged on the upper edge of the culture dish; the prepositioning device also comprises a fixing needle; the pre-positioning device comprises a clamp matched with the filling head, and the tail end of the clamp is connected with a handle.
Furthermore, a fixing device is arranged on the perfusion branch pipe, and the fixing device comprises a clamping mechanism which is detachably connected with one end of the perfusion branch pipe close to the perfusion head; the clamping mechanism comprises a threaded column, the bottom end of the threaded column is fixedly connected with a hinge ring coaxial with the threaded column, the hinge ring is fixedly connected with at least three hinge seats, each hinge seat is hinged with a shaft sleeve, and each shaft sleeve is connected with a steering structure and a clamping jaw; each steering structure comprises an inner limiting rod perpendicular to the axial direction of the shaft sleeve, one end, far away from the shaft sleeve, of each inner limiting rod is fixedly connected with an outer limiting rod perpendicular to the inner limiting rod, and each outer limiting rod is perpendicular to the axial direction of the shaft sleeve; the clamping mechanism further comprises a limiting structure matched with the steering structure.
Furthermore, the limiting structure comprises an inner limiting block matched with the inner limiting rod and an outer limiting block matched with the outer limiting rod; the inner limiting block is a round table-shaped structure with a threaded hole in the center, the inner limiting block is in threaded connection with the threaded column, the bottom end of the inner limiting block is smaller than the top end of the inner limiting block, and the inner limiting block is arranged between the inner limiting rod and the threaded column; the threaded column is fixedly connected with a threaded barrel coaxial with the threaded column, the outer limiting block is of a cylindrical structure screwed in the threaded barrel, a circular truncated cone-shaped outer limiting hole is formed in the center of the outer limiting block, and the top end of the outer limiting hole is smaller than the bottom end of the outer limiting hole; the inner limiting block, the outer limiting block and the threaded cylinder are all coaxial with the threaded column.
Furthermore, each clamping jaw comprises a telescopic rod parallel to the outer limiting rod, one end of each telescopic rod is fixedly connected with the shaft sleeve, the other end of each telescopic rod is fixedly connected with an upper clamping rod parallel to the inner limiting rod, and one end of each upper clamping rod, which is far away from the telescopic rod, is rotatably connected with a lower clamping rod; soft friction heads are uniformly distributed on each upper clamping rod and each lower clamping rod.
Compared with the prior art, the invention has the following beneficial effects:
1. the heating container top end opening can be used as an experiment platform for placing isolated myocardial tissues, and perfusate can directly flow into the heating container from the top end opening after perfusate is perfused into the isolated myocardial tissues, so that the heating container is convenient to clean. The heating container is arranged to be a double-wall structure to form a heating cavity, and the spiral heating pipe is placed in the heating cavity, so that the heating container can heat the perfusate in the heating pipe. The heating container has the functions of an experiment platform and a heating perfusate, so that the isolated myocardial tissue perfusion device is simpler and is convenient for experiment operation. The pre-positioning device can connect the perfusion head with myocardial tissue for experiment, and then perfuse the liquid in the liquid container into the myocardial tissue to confirm whether the perfusion head is inserted into the artery, and fix the perfusion head with the myocardial tissue after confirming that the perfusion head is inserted in place. The perfusion branch pipe is detached from the perfusion main pipe and then connected to the perfusion connector, so that the liquid medicine can be conveniently and accurately perfused into the artery.
2. In the prior art, after the perfusate is perfused into the isolated myocardial tissue, the gas in the perfusate can be perfused into the isolated myocardial tissue, and the gas can influence the accuracy of the experimental result. The exhaust pipe is connected with the opening at the top end of the heating pipe, so that redundant gas in the heated liquid medicine can be exhausted, the gas perfusion into isolated myocardial tissues is reduced, and the experimental result is more accurate.
3. The invention is provided with the steering pipe, and the perfusate after exhausting is heated again, so that the perfusate after exhausting is prevented from being cooled. Simultaneously the drain pipe not with fill interface lug connection, use to turn to the pipe and can set up the interface that fills in heating container open-top's edge with the liquid outlet after filling interface connection, avoid filling the interface and influence placing of separation cardiac muscle tissue.
4. Set up negative pressure hose and negative pressure pump, can take out the perfusate that flows out to heating container from separation cardiac muscle tissue when the experiment, avoid the perfusate that flows to influence the experiment, make the experimentation more stable. The lead protective tube is arranged, and leads for measuring electric signals can penetrate through the lead protective tube to be connected with the isolated myocardial tissue from the heating container. The circulating water bath heating device can keep the temperature in the heating cavity stable, and further keep the temperature of the perfusate stable. The transparent glass material is convenient for observing the experimental process.
5. The intravenous drip cup can conveniently observe whether liquid smoothly flows to the artery, and whether the filling head is inserted in place is conveniently confirmed. The hose is convenient to operate, and the perfusion head can be conveniently inserted into myocardial tissues from different angles.
6. The insertion head is in a reducing pipe shape, so that the insertion head can be inserted into an artery more conveniently. The connecting pipe is made of hard material, so that the soft filling branch pipe is not closed after being clamped by the clamp, and the filling branch pipe and the inserting head are kept stable and smooth. The inclined supporting table is arranged to facilitate liquid to flow out to the bottom of the culture dish, and the myocardial tissue experiment on the upper portion is not affected.
7. Because the insertion head is smaller and inconvenient to hold, the clamp with the handle is arranged to facilitate the holding of the handle to clamp the connecting pipe, so that the insertion head can be inserted into the artery, and the operation is facilitated. The arrangement of the included angle between the insertion head and the connecting pipe is more convenient to operate.
8. The clamping device is detachably connected with the perfusion branch pipe, so that the perfusion head can be inserted into an artery without being influenced; and after the filling head is inserted into the artery, the clamping device is connected to the filling branch pipe to clamp the myocardial tissue, the filling head is connected with the myocardial tissue together, and the loosening of the filling head is avoided.
9. The limiting structure is arranged to limit the rotation angle of the steering structure, the outer peripheral surface of the inner limiting block can be in contact with the top end of the inner limiting rod, and the minimum distance between the top end of the inner limiting rod and the threaded column is limited; the inner peripheral surface of the outer limiting block can be in contact with one end, far away from the inner limiting rod, of the outer limiting rod to limit the maximum distance between the outer limiting rod and the threaded column; the cooperation of interior stopper and outer stopper can be with turning to the structure restriction at specific angle, makes the stable centre gripping cardiac muscle tissue of clamping jaw. And the inner limiting block and the outer limiting block are both in threaded connection and can be adjusted by screwing with one hand. The clamping jaws are provided with telescopic rods, so that the positions of the upper clamping rod and the lower clamping rod can be adjusted, and the upper clamping rod and the lower clamping rod can clamp myocardial tissues at proper positions; the friction head can increase the friction force, so that the clamping of the upper clamping rod and the lower clamping rod is firmer.
Drawings
FIG. 1 is a schematic view of a perfusion apparatus according to example 1 of the present invention;
fig. 2 is an isometric view of the internal structure of a heating chamber in accordance with embodiment 1 of the present invention;
fig. 3 is a front view of the internal structure of the heating chamber according to embodiment 1 of the present invention;
FIG. 4 is a schematic view of a steering tube according to embodiment 1 of the present invention;
FIG. 5 is a schematic view showing a state of use of the negative pressure tube in embodiment 1 of the present invention;
FIG. 6 is a schematic diagram of a pre-positioning device according to embodiment 1 of the present invention;
FIG. 7 is a schematic view of a culture dish according to example 1 of the present invention;
FIG. 8 is a schematic view showing the connection of an insertion head and a perfusion manifold in example 1 of the present invention;
FIG. 9 is a schematic view of a clip according to example 1 of the present invention;
FIG. 10 is a schematic view of a fixing apparatus according to embodiment 3 of the present invention;
FIG. 11 is a sectional view of a holding mechanism according to embodiment 3 of the present invention;
FIG. 12 is a cutaway isometric view of a clamping mechanism of embodiment 3 of the present invention;
fig. 13 is a schematic view of a connecting groove in embodiment 3 of the present invention.
In the figure: the device comprises a heating container 1, an inner wall 2, an outer wall 3, a water inlet 4, a water outlet 5, a heating pipe 6, a liquid inlet 7, an exhaust port 8, an exhaust pipe 9, a liquid outlet 10, a transition arc section 11, a liquid inlet straight section 12, a liquid outlet straight section 13, a filling interface 14, a negative pressure hose 15, a negative pressure pump 16, a lead protection pipe 17, an adapter pipe 18, a filling main pipe 19, a filling branch pipe 20, a liquid container 21, a venous drip cup 22, an insertion head 23, a clamp 24, a handle 25, a connecting pipe 26, a culture dish 27, a supporting table 28, a movable joint 29, a threaded column 30, a connecting hole 31, a hinge ring 32, a shaft shoulder 33, a shaft sleeve 34, an inner limiting rod 35, an outer limiting rod 36, a connecting groove 37, a supporting cylinder 38, a connecting block 39, an inner limiting block 40, an outer limiting block 41, a threaded cylinder 42, an outer limiting hole 43, a telescopic rod 44, an upper clamping rod 45, a lower clamping rod 46 and a rotating handle 47.
Detailed Description
Example 1
A myocardial tissue perfusion experiment processing device, as shown in figures 1-9, comprises a perfusion device and a pre-positioning device detachably connected with the perfusion device; as shown in fig. 1, the perfusion device comprises a heating container 1 with an opening at the top end, the heating container 1 is a double-layer structure comprising an inner wall 2 and an outer wall 3, and a cavity formed by the outer wall 3 and the inner wall 2 is a heating cavity; a water inlet 4 and a water outlet 5 are arranged on the outer wall 3, and the heating cavity is communicated with the outside of the container through the water inlet 4 and the water outlet 5; the filling device comprises a heating pipe 6 arranged between the inner wall 2 and the outer wall 3, the heating pipe 6 is of a spiral structure, and the heating pipe 6 is connected with a filling connector 14; the pre-positioning device comprises a filling branch pipe 20 detachably connected with the filling interface 14 and a filling main pipe 19 detachably connected with the filling branch pipe 20, wherein one end, far away from the filling branch pipe 20, of the filling main pipe 19 is connected with a liquid container 21, and one end, far away from the filling main pipe 19, of the filling branch pipe 20 is connected with a filling head.
As shown in fig. 2-4, the outer wall 3 is provided with a liquid inlet 7 communicated with the bottom opening of the heating pipe 6; an exhaust port 8 is formed in the top of the outer wall 3, an exhaust pipe 9 is connected between the exhaust port 8 and the opening in the top end of the heating pipe 6, the exhaust port 8 is connected with the top of the exhaust pipe 9, the outside of the container is communicated with the exhaust pipe 9, and gas at the top of the exhaust pipe 9 is exhausted; the lower part of the exhaust pipe 9 is connected with a liquid outlet 10, and the liquid outlet 10 is arranged on the inner wall 2.
The filling device comprises a steering pipe, and the steering pipe is arranged in the heating container 1; the steering tube comprises a transition arc section 11 arranged at the bottom of the heating container 1, and the transition arc section 11 is arranged between the inner wall 2 and the outer wall 3; the steering tube also comprises a liquid inlet straight section 12 and a liquid outlet straight section 13 which are respectively connected with the two ends of the transition arc section 11; one end of the liquid inlet straight section 12 far away from the transition arc section 11 is connected with the liquid outlet 10, and one end of the liquid outlet straight section 13 far away from the transition arc section 11 is connected with the filling interface 14. The filling port 14 is arranged at the top opening of the heating container 1, and the liquid outlet straight section 13 is arranged in the heating container 1 and clings to the inner wall 2. The upper portion of heating container 1 is provided with wire protective tube 17, and wire protective tube 17 one end is located outside heating container 1, and the other end sets up on inner wall 2 and communicates in with heating container 1.
As shown in fig. 5, the perfusion apparatus further includes a negative pressure hose 15, and a negative pressure pump 16 is connected to the negative pressure hose 15. The water inlet 4 and the water outlet 5 are connected with a circulating water bath heating device; the liquid inlet 7 is connected with a perfusion liquid conveying device; the heating container 1, the heating pipe 6, the steering pipe and the lead protecting pipe 17 are all made of transparent glass materials.
As shown in FIG. 6, the main irrigation pipe 19 is provided with an intravenous drip cup 22, and the branch irrigation pipe 20 and the main irrigation pipe 19 are both flexible pipes. As shown in fig. 8, the filling head includes a connection pipe 26 connected in the filling branch pipe 20, the connection pipe 26 being made of a hard material; one end of the connecting pipe 26, which is far away from the perfusion branch pipe 20, is connected with an inserting head 23, and an included angle between the inserting head 23 and the connecting pipe 26 is an obtuse angle; the end of the insertion head 23 connected to the irrigation manifold 20 is larger in diameter than the end of the insertion head 23 remote from the irrigation manifold 20. One end of the perfusion branch pipe 20 connected with the perfusion main pipe 19 is provided with a movable joint 29, and the perfusion branch pipe 20 is respectively connected with the perfusion main pipe 19 and the perfusion interface 14 through the movable joint 29.
As shown in fig. 7, the pre-positioning device comprises a culture dish 27, a support platform 28 is arranged in the culture dish 27, the support platform 28 is a chamfered cylindrical structure coaxial with the culture dish 27, and the highest point of the support platform 28 is on the upper edge of the culture dish 27; the support platform 28 is a resin material poured into the culture dish 27. The pre-positioning device further comprises a fixing pin. As shown in fig. 9, the pre-positioning means comprises a clip 24 which is fitted to the pouring head, and a handle 25 in the form of a long rod is attached to the rear end of the clip 24.
Example 2
The embodiment is a myocardial tissue perfusion experiment processing device adopting the following experiment method, which comprises the following steps:
step 1, taking myocardial tissue experimental materials, firstly fixing myocardial tissues on a support platform 28 by using a fixing needle, clamping a clamp 24 on a perfusion branch pipe 20 outside a connecting pipe 26, inserting an insertion head 23 into an artery of the myocardial tissues by holding a handle 25 with a hand, and then perfusing liquid in a liquid container 21 into the myocardial tissues through a perfusion main pipe 19, the perfusion branch pipe 20 and a perfusion head.
Step 2, if the liquid in the drip cup drops smoothly, the insertion head 23 is proved to be inserted in place, at this time, the insertion head 23 is fixed with the myocardial tissue, and the perfusion branch pipe 20 is detached from the perfusion main pipe 19. If the drip cup is not capable of smoothly dripping the liquid, it is proved that the insertion head 23 is not inserted in place, and the insertion head 23 needs to be reinserted into the artery of the myocardial tissue until the drip cup is capable of smoothly dripping the liquid.
Step 3, heating the heating cavity; and starting the circulating water bath heating device, continuously pumping hot water into the heating cavity from the water inlet 4, and simultaneously, flowing out the hot water from the water outlet 5 to the circulating water bath heating device for reheating so as to keep the temperature in the heating cavity stable.
Step 4, perfusing perfusate; the negative pressure tube is placed at the bottom of the heating container 1, the isolated cardiac muscle tissue connected with the perfusion head is fixed at the opening at the top end of the heating container 1, the perfusion head is connected with the perfusion interface 14, and the measuring instrument is connected to the isolated cardiac muscle tissue. The perfusate conveying device is started, the perfusate conveying device adds perfusate into the heating pipe 6 from the liquid inlet 7, the perfusate flows through the heating pipe 6, the exhaust pipe 9, the liquid outlet 10, the adapter pipe 18, the liquid inlet straight section 12, the transition arc section 11, the liquid outlet straight section 13, the perfusion interface 14 and the perfusion head in the isolated myocardial tissue in sequence, and then flows out of the isolated myocardial tissue into the heating container 1. The experimental data are measured while the negative pressure pump 16 is activated to pump out the perfusion fluid flowing out into the heating container 1.
Example 3
The rest of this embodiment is the same as embodiment 1, except that, as shown in fig. 10-13, a fixing device is provided on the filling manifold 20, and the fixing device includes a clamping mechanism detachably connected to one end of the filling manifold 20 close to the filling head; one end of the filling branch pipe 20 close to the filling head is defined as a bottom end, and the other end is defined as a top end; as shown in fig. 10-12, the clamping mechanism includes a threaded column 30 sleeved on the pipe filling branch pipe 20, a connection hole 31 is formed in the center of the threaded column 30, the threaded column is sleeved on the pipe filling branch pipe 20 through the connection hole 31, a hinge ring 32 coaxial with the threaded column 30 is fixedly connected to the bottom end of the threaded column 30, the cross section of the hinge ring 32 is circular, four hinge seats are fixedly connected to the hinge ring 32, each hinge seat includes a hinge shaft and a shoulder 33 at two ends of the hinge shaft, and the axes of the hinge shaft and the shoulder 33 are perpendicular to the axis of the threaded column 30. Each hinge is hinged with a shaft sleeve 34, and the shaft sleeve 34 and the hinge shaft are coaxially arranged; each shaft sleeve 34 is connected with a steering structure and a clamping jaw; each steering structure comprises an inner limiting rod 35 perpendicular to the axial direction of the shaft sleeve 34, the inner limiting rod 35 is arranged at the top end of the shaft sleeve 34, one end, far away from the shaft sleeve 34, of each inner limiting rod 35 is fixedly connected with an outer limiting rod 36 perpendicular to the inner limiting rod 35, and each outer limiting rod 36 is perpendicular to the axial direction of the shaft sleeve 34; the clamping mechanism further comprises a limiting structure matched with the steering structure.
As shown in fig. 10 and 13, a hemispherical connecting groove 37 is formed in the inner wall 2 of the threaded column 30, one end of the filling branch pipe 20 close to the filling head is fixedly connected with a supporting cylinder 38, a connecting block 39 matched with the connecting groove 37 is connected to the supporting cylinder 38, the head end of the connecting block 39 is of a hemispherical structure, the connecting block 39 slides on the supporting cylinder 38 along the radial direction of the supporting cylinder 38, a spring is connected between the connecting block 39 and the supporting cylinder 38 along the sliding direction, so that the connecting block 39 can retract into the supporting cylinder 38 after being squeezed, and the head end of the connecting block 39 can be ejected after the pressure is removed; the connecting block 39 and the connecting groove 37 are male and female heads of the prior art, and can be used for realizing detachable connection, which is not shown in detail in the figure.
As shown in fig. 12-13, the limiting structure includes an inner limiting block 40 engaged with the inner limiting rod 35, and further includes an outer limiting block 41 engaged with the outer limiting rod 36; the inner limiting block 40 is a circular truncated cone-shaped structure with a threaded hole in the center, the inner limiting block 40 is sleeved on the threaded column 30 and is in threaded connection with the threaded column 30, the bottom end of the inner limiting block 40 is smaller than the top end, and the inner limiting block 40 is arranged between the inner limiting rod 35 and the threaded column 30; the threaded column 30 is fixedly connected with a threaded cylinder 42 coaxial with the threaded column, the outer limiting block 41 is of a cylindrical structure screwed in the threaded cylinder 42, a circular truncated cone-shaped outer limiting hole 43 is formed in the center of the outer limiting block 41, and the top end of the outer limiting hole 43 is smaller than the bottom end; the inner limiting block 40, the outer limiting block 41 and the threaded cylinder 42 are all coaxial with the threaded column 30. The diameters of the threaded cylinder 42, the outer stop block 41, the inner stop block 40 and the threaded post 30 are sequentially reduced.
Each clamping jaw comprises a telescopic rod 44 parallel to the outer limiting rod 36, the length of each telescopic rod 44 can be changed, one end of each telescopic rod 44 is fixedly connected with the shaft sleeve 34, the other end of each telescopic rod 44 is fixedly connected with an upper clamping rod 45 parallel to the inner limiting rod 35, and one end, far away from the telescopic rod 44, of each upper clamping rod 45 is rotatably connected with a lower clamping rod 46; the angle between the lower clamping rod 46 and the upper clamping rod 45 is adjustable, the upper clamping rod 45 and the lower clamping rod 46 can be connected by using a bolt and a nut, the angle can be adjusted by loosening the nut, and the fixed angle is increased by tightening the nut; soft friction heads are uniformly distributed on each of the upper clamping rod 45 and the lower clamping rod 46.
When in use, the other parts of the present embodiment are the same as those of embodiment 2, except that: before step 1, the inner limiting block 40 is rotated by holding the rotating handle 47 by hand, so that the inner limiting block 40 moves upwards, and the inner limiting block 40 is far away from the inner limiting rod 35; then, the outer limiting block 41 is rotated to enable the outer limiting block 41 to move downwards, the outer limiting block 41 moves downwards and extrudes the outer limiting rod 36, the outer limiting rod 36 rotates towards the direction close to the threaded column 30, and the clamping jaw is driven to rotate towards the direction far away from the threaded column 30; causing the jaws to open. The length of the telescopic rod 44 and the angle between the lower clamping rod 46 and the upper clamping rod 45 are adjusted according to the myocardial tissues with different sizes. In step 2, after the liquid in the drip cup drips smoothly, the insertion head 23 is fixed with the heart machine tissue, and the specific steps are as follows: holding the insertion head 23 in one hand and the threaded stud 30 on the support cylinder 38 with the other hand connects the connecting slot 37 to the connecting block 39 and thus the threaded stud 30 to the infusion branch 20 and the insertion head 23. Then, the outer limiting block 41 is rotated to move the outer limiting block 41 upwards, the inner limiting block 40 is rotated to move the inner limiting block 40 downwards and extrude the joint of the inner limiting rod 35 and the outer limiting rod 36, the inner limiting rod 35 is rotated towards the direction far away from the threaded column 30, and the clamping jaw is driven to rotate towards the direction close to the threaded column 30; the jaws are contracted. Then, the outer limiting block 41 is rotated to move the outer limiting block 41 downward, the outer limiting block 41 contacts with the outer limiting rod 36, the outer limiting block 41 and the inner limiting block 40 fix the rotation angle of the inner limiting rod 36 and the outer limiting rod 35, and the myocardial tissue is clamped and fixed.
Claims (9)
1. A myocardial tissue perfusion experiment processing device is characterized by comprising a perfusion device and a pre-positioning device which is detachably connected with the perfusion device; the filling device comprises a heating container with an opening at the top end, the heating container is of a double-layer structure comprising an inner wall and an outer wall, and a water inlet and a water outlet are formed in the outer wall; the filling device comprises a heating pipe arranged between the inner wall and the outer wall, the heating pipe is of a spiral structure, and the heating pipe is connected with a filling interface; the pre-positioning device comprises a filling branch pipe detachably connected with the filling interface and a filling main pipe detachably connected with the filling branch pipe, one end of the filling main pipe far away from the filling branch pipe is connected with a liquid container, and one end of the filling branch pipe far away from the filling main pipe is connected with a filling head.
2. The experimental treatment apparatus for myocardial tissue perfusion according to claim 1, wherein the outer wall is provided with a liquid inlet communicated with a bottom opening of the heating tube; an exhaust port is arranged at the top of the outer wall, an exhaust pipe is connected between the exhaust port and the opening at the top end of the heating pipe, a liquid outlet is connected to the lower part of the exhaust pipe, and the liquid outlet is arranged on the inner wall; the perfusion device comprises a steering tube; the steering tube comprises a transition arc section arranged at the bottom of the heating container, and the transition arc section is arranged between the inner wall and the outer wall; the steering pipe also comprises a liquid inlet straight section and a liquid outlet straight section which are respectively connected with the two ends of the transition arc section; the one end that transition segmental arc was kept away from to the straight section of feed liquor is connected with the liquid outlet, the one end that transition segmental arc was kept away from to the straight section of play liquid is provided with fills the interface.
3. The experimental myocardial tissue perfusion treatment device of claim 2, wherein the perfusion device comprises a negative pressure hose to which a negative pressure pump is connected; the upper portion of heating container is provided with the wire protective tube, wire protective tube one end is located outside the heating container, the other end setting on the inner wall and with heating container in intercommunication.
4. A myocardial tissue perfusion experimental treatment device according to any one of claims 1 to 3, wherein the main perfusion tube is provided with a venous drip cup, and the perfusion branch tube and the main perfusion tube are both flexible tubes.
5. The experimental myocardial tissue perfusion treatment device according to claim 4, wherein the perfusion head includes a connection tube connected within a perfusion branch tube, the connection tube being made of a hard material; one end of the connecting pipe, which is far away from the perfusion branch pipe, is connected with an inserting head, and an included angle between the inserting head and the connecting pipe is an obtuse angle; the diameter of one end of the insertion head connected with the perfusion branch pipe is larger than that of one end of the insertion head far away from the perfusion branch pipe.
6. The experimental myocardial tissue perfusion treatment device of claim 5, wherein the pre-positioning device includes a culture dish, a support platform is disposed in the culture dish, the support platform is a chamfered cylindrical structure coaxial with the culture dish, and the highest point of the support platform is on the upper edge of the culture dish; the pre-positioning device further comprises a fixing pin.
7. The experimental myocardial tissue perfusion treatment device according to claim 6, wherein a fixing device is disposed on the perfusion branch tube, and the fixing device comprises a clamping mechanism detachably connected with one end of the perfusion branch tube close to the perfusion head; the clamping mechanism comprises a threaded column, the bottom end of the threaded column is fixedly connected with a hinge ring coaxial with the threaded column, the hinge ring is fixedly connected with at least three hinge seats, each hinge seat is hinged with a shaft sleeve, and each shaft sleeve is connected with a steering structure and a clamping jaw; each steering structure comprises an inner limiting rod perpendicular to the axial direction of the shaft sleeve, and one end, far away from the shaft sleeve, of each inner limiting rod is fixedly connected with an outer limiting rod perpendicular to the inner limiting rod; the clamping mechanism further comprises a limiting structure matched with the steering structure.
8. The myocardial tissue perfusion experiment treatment device of claim 7, wherein the limiting structure includes an inner limiting block cooperating with the inner limiting rod, and further includes an outer limiting block cooperating with the outer limiting rod; the inner limiting block is a round table-shaped structure with a threaded hole in the center, the inner limiting block is in threaded connection with the threaded column, the bottom end of the inner limiting block is smaller than the top end of the inner limiting block, and the inner limiting block is arranged between the inner limiting rod and the threaded column; the threaded column is fixedly connected with a threaded cylinder coaxial with the threaded column, the outer limiting block is of a cylindrical structure screwed in the threaded cylinder, a truncated cone-shaped outer limiting hole is formed in the center of the outer limiting block, and the top end of the outer limiting hole is smaller than the bottom end of the outer limiting hole; the inner limiting block, the outer limiting block and the threaded cylinder are all coaxial with the threaded column.
9. The experimental myocardial tissue perfusion treatment device according to claim 8, wherein each of the clamping jaws comprises a telescopic rod parallel to an outer limiting rod, one end of each telescopic rod is fixedly connected with the shaft sleeve, the other end of each telescopic rod is fixedly connected with an upper clamping rod parallel to an inner limiting rod, and one end of each upper clamping rod, which is far away from the telescopic rod, is rotatably connected with a lower clamping rod; soft friction heads are uniformly distributed on each of the upper clamping rod and the lower clamping rod.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210749654U (en) * | 2018-11-21 | 2020-06-16 | 郑州大学第一附属医院 | Composite filling device |
| CN212971424U (en) * | 2020-06-22 | 2021-04-16 | 天津海河标测技术检测有限公司 | Isolated heart perfusion system for animal experiments |
| CN112772632A (en) * | 2020-12-31 | 2021-05-11 | 重庆北部宽仁医院 | Isolated liver perfusion device |
| CN214758844U (en) * | 2021-02-01 | 2021-11-19 | 无锡市人民医院 | Separated small animal isolated organ perfusion container and assembly |
| TWM623040U (en) * | 2021-10-22 | 2022-02-01 | 邱柏文 | Container positioning and screening structure of filling system |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210749654U (en) * | 2018-11-21 | 2020-06-16 | 郑州大学第一附属医院 | Composite filling device |
| CN212971424U (en) * | 2020-06-22 | 2021-04-16 | 天津海河标测技术检测有限公司 | Isolated heart perfusion system for animal experiments |
| CN112772632A (en) * | 2020-12-31 | 2021-05-11 | 重庆北部宽仁医院 | Isolated liver perfusion device |
| CN214758844U (en) * | 2021-02-01 | 2021-11-19 | 无锡市人民医院 | Separated small animal isolated organ perfusion container and assembly |
| TWM623040U (en) * | 2021-10-22 | 2022-02-01 | 邱柏文 | Container positioning and screening structure of filling system |
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