CN207143258U - A kind of cell-exposure oxygen-deficient environment experimental device - Google Patents
A kind of cell-exposure oxygen-deficient environment experimental device Download PDFInfo
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- CN207143258U CN207143258U CN201720392547.XU CN201720392547U CN207143258U CN 207143258 U CN207143258 U CN 207143258U CN 201720392547 U CN201720392547 U CN 201720392547U CN 207143258 U CN207143258 U CN 207143258U
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- nutrient solution
- warm bath
- cell
- main tracheae
- bath case
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Abstract
The utility model discloses a kind of cell-exposure oxygen-deficient environment experimental device, including pure oxygen gas tank, branch air inlet pipe is installed at the top of the pure oxygen gas tank and other gas tanks, main tracheae is provided with above the branch air inlet pipe, pressure gauge is installed, and the other end of main tracheae is provided with emission-control equipment, and warm bath case is provided with below the main tracheae on the main tracheae, the side of the warm bath case is provided with current divider, and warm bath case is internally provided with nutrient solution storage chamber.It is high that the utility model devises a kind of conventional efficient, the cell-exposure oxygen-deficient environment experimental device of contrast experiment can be carried out simultaneously, the utility model is internally provided with multigroup nutrient solution storage chamber and cell culture medium warm bath case, anoxic experiment can be carried out to many animals cell simultaneously, improve conventional efficient, while the experimental result of many animals cell can be contrasted, experiment effect is more preferable, animal carcasses are replaced using in vitro culture, convenient to operate, degree of controllability is higher.
Description
Technical field
It the utility model is related to anoxic experimental technique field, specifically a kind of cell-exposure oxygen-deficient environment experimental device.
Background technology
Hypoxia test is primarily adapted for use in the diagnosis of coronary heart disease, and hypoxia test needs certain appointed condition, do not possess testing equipment,
Particularly pulmonary insufficiency, anaemia, cardiac insufficiency person should be regarded as avoiding, after normal person sucks low oxygen, arterial blood oxygen saturation
Saturation degree decline, but myocardial blood supply can be given by coronary blood flow increase it is compensatory.It is myocardium in the case after Coronary Artery Lesions
Blood supply insufficiency is but very prominent, therefore ischemic change can be shown on electrocardiogram.
Existing anoxic experiment is tested using animal carcasses mostly, and size of animal is more, not manageability, and animal individual is poor
One causes experiment effect bad greatly, and many animals can not be tested simultaneously, and conventional efficient is low, can not carry out contrast experiment,
For this it is proposed that a kind of cell-exposure oxygen-deficient environment experimental device solves the problem present on.
Utility model content
The purpose of this utility model is to provide a kind of cell-exposure oxygen-deficient environment experimental device, to solve in the prior art
Anoxic experiment tested mostly using animal carcasses, size of animal is more, not manageability, and animal individual poor one causes to test greatly
It is ineffective, many animals can not be tested simultaneously, conventional efficient is low, the problem of can not carrying out contrast experiment.
To achieve the above object, the utility model provides following technical scheme：
A kind of top of cell-exposure oxygen-deficient environment experimental device, including pure oxygen gas tank, the pure oxygen gas tank and other gas tanks
Portion is provided with branch air inlet pipe, and main tracheae is provided with above the branch air inlet pipe, and pressure gauge is provided with the main tracheae, and main
The other end of tracheae is provided with emission-control equipment, and warm bath case, the side of the warm bath case are provided with below the main tracheae
Current divider is installed, and warm bath case is internally provided with nutrient solution storage chamber, the top of the nutrient solution storage chamber, which is set up, cell training
Base is supported, the opposite side of the warm bath case is provided with nutrient solution cassette for supplying, is provided between the warm bath case and nutrient solution cassette for supplying
Nutrient solution delivery pipe, flow control valve is installed in the nutrient solution delivery pipe, and is located at Flow-rate adjustment in nutrient solution delivery pipe
Valve side is provided with booster pump, and the main tracheae is connected with warm bath case by current divider, and the bottom of the nutrient solution storage chamber is set
There is nutrient solution delivery pipe, being internally embedded for the warm bath case is provided with heater, and the bottom of warm bath case is provided with temperature controller,
The output end of the heater and the input of temperature controller are electrically connected with, and the nutrient solution storage chamber and nutrient solution delivery pipe are set altogether
Five groups are equipped with, is separated between the five tissue cultures nutrient solution storage chamber and nutrient solution delivery pipe by division board.
Preferably, control damper is installed on the main tracheae.
Compared with prior art, the beneficial effects of the utility model are：The utility model design science is reasonable, safe operation
Convenient, the utility model devises a kind of conventional efficient height, and the cell-exposure oxygen-deficient environment that can carry out contrast experiment simultaneously is real
Experiment device, the utility model are internally provided with multigroup nutrient solution storage chamber and cell culture medium warm bath case, can be simultaneously to more
Kind zooblast carries out anoxic experiment, improves conventional efficient, while can contrast the experimental result of many animals cell, experiment effect
Fruit is more preferable, replaces animal carcasses using in vitro culture, convenient to operate, degree of controllability is higher.
Brief description of the drawings
Fig. 1 is a kind of structural representation of cell-exposure oxygen-deficient environment experimental device of the utility model.
In figure：1- branch air inlet pipe, 2- pressure gauges, the main tracheaes of 3-, 4- control dampers, 5- cell culture mediums, at 6- waste gas
Manage device, 7- nutrient solutions cassette for supplying, 8- flow control valves, 9- booster pumps, 10- nutrient solutions delivery pipe, 11- warm bath case, 12- heating
Device, 13- nutrient solutions storage chamber, 14- nutrient solutions delivery pipe, 15- current dividers, 16- pure oxygens gas tank, 17- other gas tanks.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Referring to Fig. 1, in the utility model embodiment, a kind of cell-exposure oxygen-deficient environment experimental device, including purity oxygen
The top of tank 16, pure oxygen gas tank 16 and other gas tanks 17 is provided with branch air inlet pipe 1, and the top of branch air inlet pipe 1 is provided with main tracheae
3, pressure gauge 2 is installed on main tracheae 3, and the other end of main tracheae 3 is provided with emission-control equipment 6, the lower section of main tracheae 3 is set
Warm bath case 11 is equipped with, zooblast carries out exposing anoxic style in warm bath case 11, and the side of warm bath case 11 is provided with current divider
15, and warm bath case 11 is internally provided with nutrient solution storage chamber 13, the top of nutrient solution storage chamber 13, which is set up, cell culture medium 5, carefully
Cultured zooblast is placed with born of the same parents' culture medium 5, the opposite side of warm bath case 11 is provided with nutrient solution cassette for supplying 7, nutrient solution
Cassette for supplying 7 by nutrient solution delivery pipe 10 to the delivered inside nutrient solution of nutrient solution storage chamber 13, warm bath case 11 and nutrient solution cassette for supplying
Nutrient solution delivery pipe 10 is provided between 7, flow control valve 8 is installed in nutrient solution delivery pipe 10, adjusted according to requirement of experiment
Control damper 4 and flow control valve 8, and the side of flow control valve 8 is provided with booster pump 9 in nutrient solution delivery pipe 10.
Main tracheae 3 is connected with warm bath case 11 by current divider 15, and control damper 4, nutrient solution storage are provided with main tracheae 3
The bottom of chamber 13 is provided with nutrient solution delivery pipe 14, and being internally embedded for warm bath case 11 is provided with heater 12, and warm bath case 11
Bottom temperature controller is installed, temperature controller adjusts the temperature of heater 12, the output end of heater 12 and temperature controller it is defeated
Enter end to be electrically connected with, nutrient solution storage chamber 13 and nutrient solution delivery pipe 14 are provided with five groups altogether, five tissue culture nutrient solution storage chambers 13 and culture
Separated between liquid delivery pipe 14 by division board.
Operation principle of the present utility model is：Before experiment, warm bath case 11 is opened, will be cultured thin with experimental cell
Born of the same parents' culture medium 5 is placed on the top of nutrient solution storage chamber 13, then closes warm bath case 11, and heater 12 is adjusted by temperature controller
Temperature, control damper 4 and flow control valve 8 are adjusted according to requirement of experiment, open nutrient solution cassette for supplying 7, opens purity oxygen
Tank 16 and other gas tanks 17, two kinds of gases enter warm bath case 11 by main tracheae 3 and current divider 15 and zooblast are exposed
Anaerobic environment is tested.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and
And in the case of without departing substantially from spirit or essential attributes of the present utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new
The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained
All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation
Involved claim.
Claims (2)
1. a kind of cell-exposure oxygen-deficient environment experimental device, including pure oxygen gas tank (16), it is characterised in that the pure oxygen gas tank
(16) branch air inlet pipe (1) and at the top of other gas tanks (17) is installed, main tracheae is provided with above the branch air inlet pipe (1)
(3) pressure gauge (2), is installed on the main tracheae (3), and the other end of main tracheae (3) is provided with emission-control equipment (6),
Warm bath case (11) is provided with below the main tracheae (3), the side of the warm bath case (11) is provided with current divider (15), and temperature
Bath cabinet (11) is internally provided with nutrient solution storage chamber (13), and the top of the nutrient solution storage chamber (13), which is set up, cell culture medium
(5), the opposite side of the warm bath case (11) is provided with nutrient solution cassette for supplying (7), the warm bath case (11) and nutrient solution cassette for supplying
(7) nutrient solution delivery pipe (10) is provided between, flow control valve (8) is installed, and train on the nutrient solution delivery pipe (10)
Flow control valve (8) side is provided with booster pump (9), the main tracheae (3) and warm bath case in nutrient solution delivery pipe (10)
(11) connected by current divider (15), the bottom of the nutrient solution storage chamber (13) is provided with nutrient solution delivery pipe (14), the temperature
Being internally embedded for bath cabinet (11) is provided with heater (12), and the bottom of warm bath case (11) is provided with temperature controller, the heating
The output end of device (12) and the input of temperature controller are electrically connected with, the nutrient solution storage chamber (13) and nutrient solution delivery pipe (14)
Five groups are provided with altogether, are separated between the five tissue cultures nutrient solution storage chamber (13) and nutrient solution delivery pipe (14) by division board.
A kind of 2. cell-exposure oxygen-deficient environment experimental device according to claim 1, it is characterised in that the main tracheae
(3) control damper (4) is installed on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720392547.XU CN207143258U (en) | 2017-04-14 | 2017-04-14 | A kind of cell-exposure oxygen-deficient environment experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720392547.XU CN207143258U (en) | 2017-04-14 | 2017-04-14 | A kind of cell-exposure oxygen-deficient environment experimental device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207143258U true CN207143258U (en) | 2018-03-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720392547.XU Expired - Fee Related CN207143258U (en) | 2017-04-14 | 2017-04-14 | A kind of cell-exposure oxygen-deficient environment experimental device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
-
2017
- 2017-04-14 CN CN201720392547.XU patent/CN207143258U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
| CN109266548B (en) * | 2018-10-11 | 2022-04-05 | 广州洁特生物过滤股份有限公司 | Dynamic cell culture device and cell culture system |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180327 |
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| CF01 | Termination of patent right due to non-payment of annual fee |