CN202175680U - Coculture double-channel mechanical force loading flow chamber device for in vitro cells - Google Patents
Coculture double-channel mechanical force loading flow chamber device for in vitro cells Download PDFInfo
- Publication number
- CN202175680U CN202175680U CN 201120153530 CN201120153530U CN202175680U CN 202175680 U CN202175680 U CN 202175680U CN 201120153530 CN201120153530 CN 201120153530 CN 201120153530 U CN201120153530 U CN 201120153530U CN 202175680 U CN202175680 U CN 202175680U
- Authority
- CN
- China
- Prior art keywords
- flow cavity
- flow
- cell
- altogether
- cultivated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Abstract
The utility model provides a coculture double-channel mechanical force loading flow chamber device for in vitro cells. The device mainly comprises five parts: a spiral cover plate, a glass cover plate, a flow chamber base, left and right connectors and a flow chamber coculture nested block. The spiral cover plate is arranged on the glass cover plate for compressing the glass cover plate. The glass cover plate is positioned above the flow chamber base and sealed by an O-ring to ensure no bacteria inside. A ring groove is formed on the upper surface of the flow chamber base and used for installing the O-ring; positioning holes are punched at the front and the rear parts inside and used for positioning the coculture nested block; and flow channels are formed on two sides of the base and communicated with a perfusion system. The left and the right connectors are threadedly connected with the chamber through a pipe. A glass slide embedded groove and a flow chamber groove are formed at the upper part of the coculture nested block and used for placing an upper-layer glass slide; and a lower-layer flow chamber groove is formed at the bottom and used for placing a lower-layer glass slide. The flow chamber device is connected with the perfusion system, the cells are arranged in the flow chamber, the released molecules are vertically diffused in the culture solution, the diffusion distance is small, and the purpose of coculture is achieved.
Description
Technical field
The present invention relates to biomechanics engineering research field, promptly relate to and a kind ofly can carry out common cultivation and simultaneously two kinds of cells loaded the flow cavity device of shear-stresses two kinds of cells.
Background technology
Biomechanics more and more receives the attention of domestic and international scientific circles as an emerging crossing domain subject, and flow cavity also is widely used in the cyto-mechanics research in the external flowing environment as a kind of mechanical loading unit.Initial steady flow flow cavity is by people such as Frangos, Eskin design (Eskin SG; Ives C L, Mcintire LV, et al.Response of cultured endothelial cells to steady flow [J] .Microvasc Res; 1984,28:87-94; Frangos JA; Eskin SG, Mcintire LV, et al.Flow effect on prostacyclin production by cultured human endothelial cues [J] .Science; 1985; 227:1477-1479.), in the middle of the research, the employed flow cavity of scientist is this single channel parallel flat flow chamber in early days.
In recent ten years, common single channel parallel flat flow chamber can not satisfy the needs of experiment fully, and the various flow cavities that are used for the different experiments demand begin to occur.Pulsating flow input type flow cavity (Helmlinger G like people such as the biomechanics experiment chamber Helmlinger G of U.S. Georgia engineering college design; Geiger RV; Schreck S; Et al.Efects of pulsatile flow on cultured vascular endothelial cell morphology [J] .ASME J Biomech Engr; 1991,01231650.4) and the non-parallel flat flow chamber (application number: 01231652.0) in the cell in vitro culture apparatus 113:123-131.), the improved cell in vitro of the money people of Inst. of Mechanics, CAS congruence people cultivates parallel cylinder flow cavity (application number:.But this type flow cavity still is the single channel flow cavity, only can satisfy the requirement of a kind of cell of vitro culture.
The development that deepens continuously along with the application foundation science; For better studying the pathways metabolism of cell; Influencing each other between when scientists is more and more paid attention to clone not of the same race and under same environment, cultivated altogether, for example endotheliocyte and myocyte's common cultivation (Agelikie G, Vouyouka; Lifeng Lin; Et al.Pressure and endothelial coculture upregulate myocytic Fas-FasL pathway and induce apoptosis by way of direct and paracrine mechanisms [J] .The American Journal of Surgery, 2005,190:780-786.); Therefore cultivate the flow cavity device altogether and also paid attention to by scientists gradually, and various ways occurs.The flow cavity that can be used for co-culture of cells at present is for example: common cultivation flow cavity (the G.Ed Rainger of people such as G.Ed Rainger design; Philip Stone, Clare MM, et al.A novel system for investigating the ability of smooth muscle cells and fibroblasts to regulate adhesion of flowing leukocytes to endothelial cells [J] .Journal of Immunological Methods; 2001; 255:73-82.), and Gianni Ciofani, dynamic cultivation dual cavity (the Gianni C of people such as Antonio Migliore design; Antonio M; Vittoria R, et al.Bicompartmental device for dynamic cell coculture:design, realisation and preliminary results [J] .Journal of Bioscience and Bioengineering; 2008,105 (5): 536-544.) etc.
But how many above-mentioned two kinds of flow cavities exist defective.People's such as G.Ed Rainger common cultivation flow cavity only can apply shearing stress to a kind of cell of endotheliocyte, narrow application range, and it is sex-limited to have an Agency for Science, Technology & Research, and stand under load when can not realize two kinds of cells; People's such as Gianni Ciofani dynamic cultivation dual cavity can satisfy dynamically cultivating altogether of two kinds of cells; But its dual cavity is designed by biochemical medicine fields such as research pharmacokinetics, molecular biology and drug delivery experiments; Only can realize hanging down flow cultivates altogether; The ratio of width to height is little, and it is slow that the flow shearing force is little, the chamber inner cell is cultivated the bottom flow velocity, can not realize pair cell in the biomechanics is applied the requirement of mechanical load.All in addition devices all can not carry out the function expansion.
Summary of the invention
The object of the present invention is to provide a kind of cell in vitro to cultivate the double flow duct mechanical force altogether and load the flow cavity device.Be used for two kinds of cells and cultivate, it is characterized in that comprising in the shearing that receives jointly of same environment:
Spiral cover plate: be placed on the glass cover-plate, be used for the pressed glass cover plate;
Glass cover-plate: fit tightly with the O type circle on the flow cavity susceptor body, be used to guarantee the sterile state of flow cavity inside, and be convenient to observe;
The flow cavity susceptor body: be used to install sealing-ring, place culturing cell lower floor's slide glass, place and cultivate nested block altogether, left and right joint be installed and linked to each other with perfusion system;
Left and right joint: use unsintered tape ST screw thread, be used to connect flow cavity device and perfusion system;
Flow cavity is cultivated nested block altogether: detachable, top is used to place the upper strata slide glass of culturing cell and forms the top flowing grooves, and the flowing grooves of bottom is used to form down the flowing grooves of confluent monolayer cells, and the both sides, front and back are equipped with tapped through hole, are used for the installing and locating long spiro nail;
Fixed block: be used for fixing slide glass.
The flow cavity installation steps in the cell experiment process have been simplified in the simple operation of this experimental installation, can increase work efficiency; But this device autoclaving guarantees when cell in vitro is cultivated pollution-free fully; Flow cavity device geometric parameter precision is high, accuracy is good; But cell in vitro single culture before the loading force does not receive the restriction of experimental installation, and cell is stressed evenly when loading on the device, and side effect is little; In this device, can be implemented in and cultivate two kinds of different cells under the external same environment altogether, the interaction between the research cell; Can utilize double flow duct to realize co-cultured cell is applied shearing force simultaneously.
For reaching above purpose and function; The technical scheme that the present invention adopts is achieved in that flow cavity device provided by the invention mainly comprises five major parts, and promptly spiral cover plate, glass cover-plate, flow cavity susceptor body, left and right joint and flow cavity are cultivated nested block altogether.Spiral cover plate 1 is placed on the glass cover-plate, is used for the pressed glass cover plate, and the screw thread design makes that installation is convenient, increases work efficiency.Glass cover-plate 2 is positioned at flow cavity susceptor body 5 tops, fits tightly with O type circle 3 on the flow cavity susceptor body, guarantees that the internal flow chamber cultivates the sterile state of nested block part altogether, and helps observing internal flow chamber working condition.Flow cavity susceptor body top is IT, and the spiral cover plate is twisted on it; Pedestal upper surface has ring groove, is used to install O type circle, contacts the sealed inside cavity with glass cover-plate; Bottom inner surface has the slide glass embedded groove, is used to place the lower floor's slide glass behind the inoculating cell; Both sides also are equipped with two cylindrical pilot holes before and after the flow cavity susceptor body inside, are used to locate common cultivation nested block; The pedestal both sides have the fluid IO channel, are connected with left and right joint 7 pipe-thread, utilize the be tightly connected pipe-thread at place of unsintered tape, guarantee joint with system sealing after base thread is connected, prevent leakage of liquid fluid system circulation time or bacterial contamination.The flow cavity susceptor body links to each other with perfusion system through joint.Flow cavity cultivates altogether that nested block 6 tops have the slide glass embedded groove and the cavity groove that flows, and is used to place the upper strata slide glass behind the inoculating cell, utilizes the cavity groove pair cell that flows to apply shear-stress; The bottom has lower floor's flow cavity groove, is used for the cell on lower floor's slide glass is applied shear-stress; Both sides were equipped with tapped through hole before and after flow cavity was cultivated nested block altogether, were used for installing and locating long spiro nail 8, made flow cavity cultivate nested block altogether and navigated to flow cavity susceptor body allocation really; It is detachable that flow cavity is cultivated nested block altogether, carries out the part repacking by different request for utilizations during for function expansion later on; Flow cavity is cultivated nested block altogether and is scabbled respectively in the flow direction, so that liquid can have the preceding cushion chamber of a shunting after flowing into; It is wide at the top and narrow at the bottom that flow cavity is cultivated nested block altogether, is convenient to install, and posts pellosil 9 at lateral margin, prevents that liquid from flowing out from both sides, is used for sealing.Flow cavity is cultivated two flow cavity chambers of the common formation of nested block and flow cavity susceptor body assembling back altogether, shunts when crossing two chambeies through the liquid flow of cushion chamber, forms two identical runners.According to the flow cavity characteristic, need to guarantee that the ratio of width to height is much larger than 1 in the flow cavity, we design the wide 30mm that is of two flow cavities up and down, and height is 0.5mm, satisfies flow cavity Experiments of Machanics requirement.Cultivate the slide glass flute length direction of principal axis both sides of nested block and pedestal altogether at flow cavity; Respectively have two fixed block grooves respectively; And be equipped with threaded hole, and be used to lay the fixed block 4 that has screw, fixedly slide glass can not be subjected to displacement it because of the fluid effect when receiving shear-stress.
Flow cavity device provided by the invention is through linking to each other with perfusion system; In cell is in the flow cavity that is full of nutrient solution; Do not receive shear stress leave standstill in the culture environment time, device up and down all is full of nutrient solution, molecule vertical disperse in the intravital nutrient solution in chamber that cell is discharged in cushion chamber and two flow cavities of two-layer flow cavity both sides; Diffusion length is little, to reach the purpose of common cultivation.When two kinds of cells when receiving shear action, the molecule of its release and meta-bolites together get into perfusion system with perfusate, and are back in the flow cavity once more through the Circulation of perfusion system.Through perfusate vertical diffusion and flow Circulation, can study influencing each other that the molecule that discharges in the culturing process of two kinds of cells after loading mechanical force and meta-bolites pair cell produce.
The present invention compares with background technology, and the useful effect that has is:
1. the flow cavity installation steps in the cell experiment process have been simplified in simple operation of the present invention, increase work efficiency, and keep cytoactive, and the device controllability is strong, the experiment good stability;
2. to adopt duraluminum or stainless steel and glass be material to this device, but HP steam pot and ultraviolet disinfection guarantee when cell in vitro is cultivated pollution-freely fully, and the flow cavity dimensional precision is high, accuracy is good;
3. can be implemented in and cultivate two kinds of different cells under the external same environment altogether, the interaction of research between the cell, and cell is stressed evenly when loading on the device, and side effect is little;
4. can realize co-cultured cell is applied shearing force simultaneously through double flow duct, the shearing force size is controlled through the perfusion system that links to each other;
5. molecule and the vertical disperse of meta-bolites that the design of bilevel two-chamber can make co-cultured cell discharge through the flow circulating transfer, can make intercellular influence each other rapidly and directly when receiving shear stress.
6. the main function part of flow cavity is detachable, does afterwards when function expansion or cavity are transformed and can process separately.
The invention provides a kind of can be under same environment external two kinds of different cells of cultivating altogether, simultaneously two kinds of cells are applied the flow cavity device of the identical double-shear stress of size.Can be used for studying the interaction of exploring between the different cells, better simulate the stressed relation of human body physiological environment inner cell, probe into iuntercellular and connect each other, have novelty and bigger scientific research promotion and application prospect.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is described further.
Fig. 1 is that cell in vitro is cultivated double flow duct flow cavity apparatus structure schematic perspective view altogether;
Fig. 2 is that cell in vitro is cultivated double flow duct flow cavity apparatus structure master sectional view altogether;
Fig. 3 is that cell in vitro is cultivated double flow duct flow cavity apparatus structure sectional view altogether;
Fig. 4 is apparatus of the present invention embodiment synoptic diagram.
Embodiment
Shown in accompanying drawing 1, accompanying drawing 2, install the flow cavity device additional.The flow cavity device comprises in the accompanying drawing: 1 spiral cover plate, 2 glass cover-plates, 3O type circle, 4 fixed blocks, 5 flow cavity susceptor bodies, 6 flow cavities are cultivated nested block, 7 left and right joints, 8 location long spiro nails altogether, the silica gel mould is used in 9 sealings.When installing additional before the work; Put into lower floor's slide glass that the inoculation that need receive shear-stress has clone A earlier; After utilizing fixed block fixing flow cavity being cultivated nested block altogether is embedded on the flow cavity susceptor body; Utilize the location long spiro nail fixing on pedestal, put into the upper strata slide glass that meets clone B that need receive shear-stress again after the location long spiro nail is tightened, utilize fixed block to fix; Respectively O type circle, glass cover-plate, spiral cover plate are installed on the flow cavity susceptor body according to diagram again, accomplish installing additional of flow cavity.Joint can be installed on the pedestal before stowage unit in advance, sterilizes as an integral high pressure with pedestal, reduces installation steps.
Embodiment 1
In the parallel flat flow chamber, the relation of shearing force, circulating fluid flow rate, circulation fluid viscosity and stream chamber size size is: τ=6 μ QF/WH
2
F depends on stream chamber the ratio of width to height (W/H), when W/H＞＞1, and F ≈ 1.Wherein, τ is stream chamber basal shear stress (dyn/cm
2), Q is flow (cm
3/ s); μ is perfusate viscosity (dyns/cm
2); H is stream chamber height (cm); W is flow cavity width (cm); L is stream chamber length (cm).In the native system, substratum contains 10%FBS, and the perfusate viscosity, mu is a kinetic viscosity, value 0.013dyns/cm
2The size W=3cm of stream chamber in the experiment, H=0.05cm, stream chamber length L=8.8cm.As shearing stress τ=15dyn/cm
2The time, Q=τ WH
2/ 6 μ F ≈ 1.44cm
3/ s=86.53ml/min.
Reynolds number Re is the non-dimensional number of reflection fluid flow characteristics. the ratio of expression mass force and stickiness power: Re=VH ρ/μ.Wherein V is chamber inner fluid mean flow rate (cm
2/ s), ρ is circulation fluid density (g/cm
3), be taken as 1g/cm
3At the mean flow rate V=Q/ of cavity ingress (WH)=9.6cm/s, under experiment condition of the present invention, Re=37. satisfies the requirement of Laminar Flow " low reynolds number ", and promptly Re＜critical Reynolds number 2000.When system works, complicated flow environment such as turbulent flow can not appear in the flow cavity, meet the required shear-stress condition of culturing cell.
Shown in accompanying drawing 3, the flow cavity device I and the perfusion bottle II of native system placed CO
2Among the constant incubator V, cell culture fluid is pumped from perfusion bottle II, behind the throttling valve IV through control nutrient solution flow by constant current peristaltic pump III; Through the ingress cushion chamber shunting in the flow cavity; Flow in the double flow duct flow cavity, two-layer clone A, B are up and down implemented shear-stress, the nutrient solution that flows through converges through the cushion chamber flow in exit again; Be back to after the outflow among the perfusion bottle II, so circulation.Perfusion bottle II has and is communicated with CO
2The gas port of constant incubator is so that keep total system to be in 5%CO
2In the cell culture environment.Throttling valve IV can regulation system flow flow, and then the shear-stress size in the control flow cavity.
In cell is in the flow cavity that is full of nutrient solution; Do not receive shear stress leave standstill in the culture environment time; All be full of nutrient solution in the cushion chamber of two-layer flow cavity both sides and two flow cavities about the device; Can make vertical disperse in the nutrient solution of molecule in cushion chamber that cell discharges, and in entering two flow chamber identical with cushion chamber.The diffusion length of molecule and meta-bolites is little, thereby reaches common culturing purposes.When two kinds of cells when receiving shear action, the molecule of its release and meta-bolites together get into perfusion system with perfusate, and are back to once more in the flow cavity through the Circulation of perfusion system, to reach two kinds of effects of common cultivation and double-shear.Through perfusate vertical diffusion and flow Circulation, can study influencing each other that the molecule that discharges in the culturing process of two kinds of cells after loading mechanical force and meta-bolites pair cell produce.
Claims (6)
1. a cell in vitro is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device, is used for two kinds of cells and cultivates in the shearing that receives jointly of same environment, it is characterized in that comprising:
Spiral cover plate: be placed on the glass cover-plate, be used for the pressed glass cover plate;
Glass cover-plate: fit tightly with the O type circle on the flow cavity susceptor body, be used to guarantee the sterile state of flow cavity inside, and be convenient to observe;
The flow cavity susceptor body: be used to install sealing-ring, place culturing cell lower floor's slide glass, place and cultivate nested block altogether, left and right joint be installed and linked to each other with perfusion system;
Left and right joint: use unsintered tape ST screw thread, be used to connect flow cavity device and perfusion system;
Flow cavity is cultivated nested block altogether: detachable, top is used to place the upper strata slide glass of culturing cell and forms the top flowing grooves, and the flowing grooves of bottom is used to form down the flowing grooves of confluent monolayer cells, and the both sides, front and back have tapped through hole, are used for the installing and locating long spiro nail;
Fixed block: be used for fixing slide glass.
2. cell in vitro according to claim 1 is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device, it is characterized in that the spiral cover plate adopts helicitic texture.
3. cell in vitro according to claim 1 is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device, it is characterized in that glass cover-plate is placed on the flow cavity susceptor body.
4. cell in vitro according to claim 1 is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device, it is characterized in that flow cavity susceptor body top is IT, and the spiral cover plate is twisted on it; The pedestal upper face has ring groove, is used to install O type circle, contacts with glass cover-plate; Lower surface has the slide glass embedded groove, is used to place the lower floor's slide glass behind the inoculating cell; Both sides are equipped with two cylindrical pilot holes before and after the inside cavity that flows, and are used to locate common cultivation nested block; The pedestal both sides have the fluid IO channel, are threaded with left and right joint, are communicated with perfusion system, are connected through pipe-thread between left and right joint and the mobile cavity.
5. cell in vitro according to claim 1 is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device; It is characterized in that; Flow cavity is cultivated nested block top altogether and is had slide glass embedded groove and mobile cavity groove; Be used to place the slide glass of upper strata inoculating cell, and utilize the cavity groove pair cell that flows to apply shear-stress; The bottom has lower floor's flow cavity groove, is used for the cell on lower floor's slide glass is applied shear-stress, according to the flow cavity characteristic, guarantees in the flow cavity the ratio of width to height greater than 1, the wide 30mm that is of two flow cavities up and down, and height is 0.5mm; Both sides were equipped with tapped through hole before and after flow cavity was cultivated nested block altogether, were used for the installing and locating long spiro nail, made flow cavity cultivate nested block altogether and navigated to flow cavity susceptor body allocation really; It is detachable that flow cavity is cultivated nested block altogether.
6. cell in vitro according to claim 1 is cultivated the double flow duct mechanical force altogether and is loaded the flow cavity device, it is characterized in that flow cavity is cultivated in the fixed block groove on nested block top altogether to be equipped with tapped through hole, is used to lay the fixed block that has screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120153530 CN202175680U (en) | 2011-04-18 | 2011-05-13 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110096171 | 2011-04-18 | ||
| CN201110096171.5 | 2011-04-18 | ||
| CN 201120153530 CN202175680U (en) | 2011-04-18 | 2011-05-13 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202175680U true CN202175680U (en) | 2012-03-28 |
Family
ID=44842493
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120153530 Expired - Fee Related CN202175680U (en) | 2011-04-18 | 2011-05-13 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
| CN2011101245036A Pending CN102229887A (en) | 2011-04-18 | 2011-05-13 | In vitro cell co-culturing double flow mechanical force loaded flow chamber apparatus |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101245036A Pending CN102229887A (en) | 2011-04-18 | 2011-05-13 | In vitro cell co-culturing double flow mechanical force loaded flow chamber apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN202175680U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229887A (en) * | 2011-04-18 | 2011-11-02 | 北京航空航天大学 | In vitro cell co-culturing double flow mechanical force loaded flow chamber apparatus |
| CN103194388A (en) * | 2013-03-22 | 2013-07-10 | 中国科学院力学研究所 | Double-layer cell co-culture device with adjustable gap |
| CN105483004A (en) * | 2016-02-22 | 2016-04-13 | 上海泉众机电科技有限公司 | Parallel plate experiment cavity device |
| CN114106983A (en) * | 2021-11-04 | 2022-03-01 | 长沙湘计海盾科技有限公司 | Microbe culture chip for spaceflight and preparation process thereof |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296326B (en) * | 2015-09-30 | 2017-05-31 | 重庆大学 | A kind of vascular fluid platform of separate chamber |
| CN105154316B (en) * | 2015-09-30 | 2017-04-12 | 重庆大学 | Blood vessel-like running cavity |
| CN107058065B (en) * | 2017-06-30 | 2019-10-25 | 成都大学 | Medical inspection microbial culture garden |
| CN109655379B (en) * | 2018-12-29 | 2022-02-01 | 潍坊医学院 | Chute plate device and measuring method for researching influence of fluid shear stress on cells |
| CN110320150A (en) * | 2019-06-08 | 2019-10-11 | 潍坊医学院 | A kind of device for cell in microscopic fluid |
| CN113234593A (en) * | 2021-03-26 | 2021-08-10 | 北京航空航天大学 | Double-layer cell culture device for differential co-culture of two cells |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1175821A (en) * | 1997-09-01 | 1999-03-23 | Res Dev Corp Of Japan | Biochemical reaction apparatus for culturing several series of cell and biochemical reaction |
| CN2516990Y (en) * | 2001-10-23 | 2002-10-16 | 中国科学院生物物理研究所 | Sample viewing device for microscope |
| CN2727183Y (en) * | 2004-04-21 | 2005-09-21 | 四川大学 | Cell fluid mechanics experimental apparatus |
| CN202175680U (en) * | 2011-04-18 | 2012-03-28 | 北京航空航天大学 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
-
2011
- 2011-05-13 CN CN 201120153530 patent/CN202175680U/en not_active Expired - Fee Related
- 2011-05-13 CN CN2011101245036A patent/CN102229887A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229887A (en) * | 2011-04-18 | 2011-11-02 | 北京航空航天大学 | In vitro cell co-culturing double flow mechanical force loaded flow chamber apparatus |
| CN103194388A (en) * | 2013-03-22 | 2013-07-10 | 中国科学院力学研究所 | Double-layer cell co-culture device with adjustable gap |
| CN103194388B (en) * | 2013-03-22 | 2014-06-11 | 中国科学院力学研究所 | Double-layer cell co-culture device with adjustable gap |
| CN105483004A (en) * | 2016-02-22 | 2016-04-13 | 上海泉众机电科技有限公司 | Parallel plate experiment cavity device |
| CN105483004B (en) * | 2016-02-22 | 2020-04-14 | 上海泉众机电科技有限公司 | Parallel flat plate experimental cavity device |
| CN114106983A (en) * | 2021-11-04 | 2022-03-01 | 长沙湘计海盾科技有限公司 | Microbe culture chip for spaceflight and preparation process thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102229887A (en) | 2011-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202175680U (en) | Coculture double-channel mechanical force loading flow chamber device for in vitro cells | |
| CN101942389A (en) | Mechanical loading flow chamber device for co-culturing in-vitro cells | |
| CN112608841B (en) | Microfluidic system for tumor organoid culture and drug experiments and application method | |
| CN104974935A (en) | device with annular micro-channel chip for cell culture | |
| Marimuthu et al. | Pumpless steady-flow microfluidic chip for cell culture | |
| RU2612904C1 (en) | Method and microfluidic chip for cell culture or cell model | |
| CN211645272U (en) | Multichannel cell stretching stress loading device | |
| CN103756898A (en) | Three-dimensional stress cell culture device capable of applying dynamic load | |
| CN113388517A (en) | Biological culture micro-fluidic chip suitable for assembling microgravity gyroscope and cell culture method thereof | |
| Offeddu et al. | Microheart: A microfluidic pump for functional vascular culture in microphysiological systems | |
| CN102296030B (en) | Flow chamber for loading disturbance flow on co-cultured cells | |
| CN104762206A (en) | In vitro cell culture device and culture method | |
| CN205635639U (en) | Cell force means based on hydrodynamic shear is amazing | |
| CN107723236A (en) | Dynamic perfusion culture systems | |
| CN103146574A (en) | High-flux microfluidic biomechanical long-term stimulation system and use thereof | |
| CN103131635B (en) | Cavity-type dynamic-filling bioreaction device | |
| CN112852627A (en) | Method for co-culturing pluripotent stem cells from human liver and pancreatic islets based on multi-organ chip | |
| CN102816695B (en) | Micro-fluidic chip and method for studying effect of fluid shearing force on cell with the micro-fluidic chip | |
| CN108467837B (en) | Visual multichannel fluid shear force cell culture device and method thereof | |
| CN105349420A (en) | Bionic microfluidic cell culture chip | |
| CN204455070U (en) | A kind of cell fluid mechanics experimental apparatus | |
| CN103103121B (en) | Cell-culture microfluidic chip | |
| CN205473829U (en) | Interact's culture apparatus between cell culture and cell | |
| CN204661733U (en) | Cell injuring model device | |
| CN201834910U (en) | Two-way perfusion mechanical experiment device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120328 Termination date: 20130513 |