CN114106983A - Microbe culture chip for spaceflight and preparation process thereof - Google Patents
Microbe culture chip for spaceflight and preparation process thereof Download PDFInfo
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- CN114106983A CN114106983A CN202111297992.5A CN202111297992A CN114106983A CN 114106983 A CN114106983 A CN 114106983A CN 202111297992 A CN202111297992 A CN 202111297992A CN 114106983 A CN114106983 A CN 114106983A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 38
- 239000011521 glass Substances 0.000 claims abstract description 27
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- 238000010438 heat treatment Methods 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 238000009629 microbiological culture Methods 0.000 claims abstract description 18
- 239000000243 solution Substances 0.000 claims abstract description 16
- 238000002347 injection Methods 0.000 claims abstract description 11
- 239000007924 injection Substances 0.000 claims abstract description 11
- 230000000813 microbial effect Effects 0.000 claims abstract 3
- 239000010410 layer Substances 0.000 claims description 21
- 239000000565 sealant Substances 0.000 claims description 21
- 239000003292 glue Substances 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 15
- 239000012945 sealing adhesive Substances 0.000 claims description 8
- 239000012790 adhesive layer Substances 0.000 claims description 6
- 239000003822 epoxy resin Substances 0.000 claims description 6
- 229920000647 polyepoxide Polymers 0.000 claims description 6
- 238000007711 solidification Methods 0.000 claims description 6
- 230000008023 solidification Effects 0.000 claims description 6
- 230000001680 brushing effect Effects 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000001879 gelation Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 230000005486 microgravity Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a microbial cultivation chip for spaceflight and a preparation process thereof, wherein the microbial cultivation chip for spaceflight comprises a heating glass bottom plate, an enclosure frame which is arranged on the heating glass bottom plate and is fixedly connected with the heating glass bottom plate in a sealing manner, a culture medium fixedly arranged in the enclosure frame and an observation window fixedly arranged at the top of the enclosure frame in a sealing manner, wherein one side of the enclosure frame is provided with a liquid injection and exhaust integrated hole, and a rubber plug is hermetically assembled at the liquid injection and exhaust integrated hole. The microbial culture chip for spaceflight and the preparation process thereof solve the sealing problem when the microbes based on the culture solution are cultured in an on-orbit mode, and improve the life safety and the electrical safety of on-orbit astronauts.
Description
Technical Field
The invention relates to the technical field of aerospace microbial culture, in particular to a microbial culture chip for aerospace and a preparation process thereof.
Background
The microorganism is cultured on the orbit so as to research the influence of space radiation, microgravity and the like on the growth process of the microorganism, and the method has great significance for food safety, pharmacy and the like. At present, in-orbit microbial culture is mainly based on an unmanned spacecraft platform, a semi-solid sealing or solid sealing mode is adopted for many culture mediums, the sealing requirement is not high, but when the microbes based on culture solution need to be cultured in an in-orbit mode, bubbles or leakage is easily generated under the influence of space environment microgravity and air pressure change due to the low sealing performance of the existing culture chip, and therefore the personal safety or the electrical safety of astronauts are influenced.
Disclosure of Invention
In view of the above, the present invention provides a microbial culture chip for aerospace and a preparation method thereof, so as to solve the sealing problem when culturing microbes based on a culture solution in an on-orbit manner.
The invention solves the problems through the following technical means:
the utility model provides a chip is cultivateed to microorganism for space flight, includes heating glass bottom plate, sets up on heating glass bottom plate and with the sealed fixed connection of heating glass bottom plate enclose the frame, fixed setting is enclosing the culture medium in the frame and sealed fixed setting is enclosing the observation window at frame top, enclose one side of frame and seted up notes liquid exhaust integrated hole, it is sealed to be equipped with the rubber buffer to annotate liquid exhaust integrated hole department.
Furthermore, the enclosing frame and the heating glass bottom plate are fixed by an adhesive layer in a sticking way.
Furthermore, the inner side and the outer side of the lap joint of the enclosure frame and the heating glass bottom plate are both provided with a sealing glue layer.
Further, enclose frame internal fixation and be provided with culture medium mounting platform, the culture medium passes through screw and culture medium mounting platform fixed mounting.
Furthermore, a sealing ring is arranged on the lap joint surface of the observation window and the surrounding frame.
Furthermore, the observation window is fixedly connected with the frame through a screw.
Furthermore, a sealing adhesive layer is filled in a gap between the observation window and the surrounding frame.
Further, the rubber buffer and enclose the gap department between the frame and fill the sealing glue layer.
A preparation process of a microbial culture chip for spaceflight comprises the following steps:
s1: brushing a layer of epoxy resin glue at the bottom of the enclosure frame, then lapping the bottom of the enclosure frame with the top of the heating glass bottom plate, after lapping, coating a circle of GD414C sealant on the inner side and the outer side of the lapping part of the enclosure frame and the heating glass bottom plate, standing, waiting for gelation to be solidified, and ensuring that the enclosure frame is fixedly connected with the heating glass bottom plate in a sealing way, wherein the epoxy resin glue is solidified to form an adhesive layer, and the GD414C glue is solidified to form a sealing adhesive layer;
s2: after the culture medium is inoculated with microorganisms, the culture medium is fixedly arranged on a culture medium mounting platform in the enclosure frame through screws;
s3: installing a sealing ring in a sealing ring installation groove at the top of the enclosure frame, covering the observation window, and fixedly connecting the observation window with the enclosure frame through a screw; filling GD414C sealant in a gap between the observation window and the enclosure frame, covering the screw with the GD414C sealant, and forming a sealant layer after solidification;
s4: injecting culture solution into the culture medium through the liquid injection and air exhaust integrated hole on the enclosing frame, observing whether bubbles exist in the culture solution through the observation window after the liquid injection is finished, and plugging a rubber plug at the liquid injection and air exhaust integrated hole after all the bubbles are exhausted;
s5: after the bubble-free state is repeatedly confirmed, a gap between the rubber plug and the enclosure frame is filled with the GD414C sealant for secondary sealing, the GD414C sealant covers the rubber plug, and a sealant layer is formed after solidification.
The invention has the beneficial effects that:
1. according to the microbial culture chip for spaceflight and the preparation process thereof, the sealing performance of the whole microbial culture chip is ensured by the matching use of the sealing glue, the sealing ring and the fixing glue, meanwhile, the thorough discharge of gas in the culture solution is ensured before the thorough sealing, the problem that bubbles or leakage is easy to generate under the influence of microgravity and air pressure change of the space environment when microbes based on the culture solution are cultured in an orbit is avoided, and the life safety and the electrical safety of on-orbit astronauts are improved; can be widely applied to the culture of space microorganisms of aircrafts such as orbit satellites, space stations, manned airships and the like or lunar scientific research stations and the like.
2. The aerospace microorganism culture chip and the preparation process effectively solve the problem of air bubble influence in graphic image monitoring of aerospace liquid microorganism culture.
3. The microbial culture chip for spaceflight and the preparation process thereof are simple to operate, do not need to be carried out by means of a special device or under a specific environment, and are good in reliability and strong in operability.
Drawings
The invention is further described below with reference to the figures and examples.
Fig. 1 is a cross-sectional view of a preferred embodiment of the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings.
Referring to fig. 1, this embodiment provides a microorganism culture chip for space flight, including heating glass bottom plate 5, set up on the heating glass bottom plate and with the sealed fixed connection of heating glass bottom plate enclose frame 9, fixed setting in enclosing the culture medium 7 of frame and sealed fixed setting at the observation window 1 that encloses the frame top, enclose one side of frame and seted up notes liquid exhaust integrated hole, it is equipped with rubber buffer 8 to annotate the sealed of liquid exhaust integrated hole department. The enclosure frame and the heating glass bottom plate are fixedly adhered through an adhesive layer 4. And sealing glue layers 6 are arranged on the inner side and the outer side of the lap joint of the enclosure frame and the heating glass bottom plate. Enclose frame internal fixation and be provided with culture medium mounting platform, culture medium 7 passes through screw 2 and culture medium mounting platform fixed assembly. The overlapping surface of the observation window 1 and the surrounding frame 9 is provided with a sealing ring 3, and the top of the surrounding frame is provided with a sealing ring mounting groove. The observation window is fixedly connected with the frame through a screw 2. And a sealing adhesive layer is filled in a gap between the observation window and the enclosure frame. And a sealing adhesive layer is filled in a gap between the rubber plug and the enclosure frame.
The embodiment also provides a preparation process of the microbial culture chip for spaceflight, which comprises the following steps:
s1: brushing a layer of epoxy resin glue at the bottom of the enclosure frame, then lapping the bottom of the enclosure frame with the top of the heating glass bottom plate, after lapping, coating a circle of GD414C sealant on the inner side and the outer side of the lapping part of the enclosure frame and the heating glass bottom plate, standing, waiting for gelation to be solidified, and ensuring that the enclosure frame is fixedly connected with the heating glass bottom plate in a sealing way, wherein the epoxy resin glue is solidified to form an adhesive layer, and the GD414C glue is solidified to form a sealing adhesive layer;
s2: after the culture medium is inoculated with microorganisms, the culture medium is fixedly arranged on a culture medium mounting platform in the enclosure frame through screws at four corners of the culture medium;
s3: a sealing ring is arranged in a sealing ring mounting groove at the top of the surrounding frame, and the sealing ring is an O-shaped sealing ring; covering the observation window, and fixedly connecting the observation window with the enclosure frame through a screw on the observation window; filling GD414C sealant in a gap between the observation window and the enclosure frame, covering the screw with the GD414C sealant, and forming a sealant layer after solidification;
s4: injecting culture solution into the culture medium through the liquid injection and air exhaust integrated hole on the enclosing frame, observing whether bubbles exist in the culture solution through the observation window after the liquid injection is finished, and plugging a rubber plug at the liquid injection and air exhaust integrated hole after all the bubbles are exhausted;
s5: after the bubble-free state is repeatedly confirmed, a gap between the rubber plug and the enclosure frame is filled with the GD414C sealant for secondary sealing, the GD414C sealant covers the rubber plug, and a sealant layer is formed after solidification.
In summary, according to the microorganism culture chip for spaceflight and the preparation process thereof, on one hand, the sealing performance of the whole microorganism culture chip is ensured by the matching use of the sealing glue, the sealing ring and the fixing glue, and simultaneously, the thorough discharge of gas in the culture solution is ensured before the thorough sealing, so that the problem that bubbles or leakage is easily generated under the influence of the microgravity and the air pressure change of the space environment when the microorganism based on the culture solution is cultured in the orbit is avoided, and the life safety and the electrical safety of the on-orbit astronauts are improved; the culture medium can be widely applied to culture of space microorganisms of aircrafts such as orbit satellites, space stations, manned airships and the like or lunar scientific research stations and the like; on the other hand, the problem of air bubble influence in graphic image monitoring of aerospace liquid microorganism culture is effectively solved. In addition, the operation is simple, the operation is carried out without a special device or a special environment, the reliability is good, and the operability is strong.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (9)
1. A microbial cultivation chip for spaceflight is characterized in that: including heating glass bottom plate, setting on heating glass bottom plate and with the sealed fixed connection of heating glass bottom plate enclose the frame, fixed setting is in the culture medium that encloses the frame and the sealed fixed observation window that sets up at enclosing the frame top, enclose one side of frame and seted up and annotate liquid exhaust integrated hole, it is equipped with the rubber buffer to annotate the sealed of liquid exhaust integrated hole department.
2. The microbial culture chip for aerospace of claim 1, wherein: the enclosure frame and the heating glass bottom plate are fixedly adhered through an adhesive layer.
3. The microbial culture chip for aerospace of claim 2, wherein: and sealing glue layers are arranged on the inner side and the outer side of the lap joint of the enclosure frame and the heating glass bottom plate.
4. The microbial culture chip for aerospace of claim 3, wherein: the enclosure frame is internally and fixedly provided with a culture medium mounting platform, and the culture medium is fixedly assembled with the culture medium mounting platform through screws.
5. The microbial culture chip for aerospace of claim 4, wherein: and a sealing ring is arranged at the lap joint surface of the observation window and the surrounding frame.
6. The microbial culture chip for aerospace of claim 5, wherein: the observation window is fixedly connected with the frame through a screw.
7. The microbial culture chip for aerospace of claim 6, wherein: and a sealing adhesive layer is filled in a gap between the observation window and the enclosure frame.
8. The microbial culture chip for aerospace of claim 7, wherein: and a sealing adhesive layer is filled in a gap between the rubber plug and the enclosure frame.
9. A preparation process of a microbial culture chip for spaceflight is characterized by comprising the following steps:
s1: brushing a layer of epoxy resin glue at the bottom of the enclosure frame, then lapping the bottom of the enclosure frame with the top of the heating glass bottom plate, after lapping, coating a circle of GD414C sealant on the inner side and the outer side of the lapping part of the enclosure frame and the heating glass bottom plate, standing, waiting for gelation to be solidified, and ensuring that the enclosure frame is fixedly connected with the heating glass bottom plate in a sealing way, wherein the epoxy resin glue is solidified to form an adhesive layer, and the GD414C glue is solidified to form a sealing adhesive layer;
s2: after the culture medium is inoculated with microorganisms, the culture medium is fixedly arranged on a culture medium mounting platform in the enclosure frame through screws;
s3: installing a sealing ring in a sealing ring installation groove at the top of the enclosure frame, covering the observation window, and fixedly connecting the observation window with the enclosure frame through a screw; filling GD414C sealant in a gap between the observation window and the enclosure frame, covering the screw with the GD414C sealant, and forming a sealant layer after solidification;
s4: injecting culture solution into the culture medium through the liquid injection and air exhaust integrated hole on the enclosing frame, observing whether bubbles exist in the culture solution through the observation window after the liquid injection is finished, and plugging a rubber plug at the liquid injection and air exhaust integrated hole after all the bubbles are exhausted;
s5: after the bubble-free state is repeatedly confirmed, a gap between the rubber plug and the enclosure frame is filled with the GD414C sealant for secondary sealing, the GD414C sealant covers the rubber plug, and a sealant layer is formed after solidification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111297992.5A CN114106983A (en) | 2021-11-04 | 2021-11-04 | Microbe culture chip for spaceflight and preparation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111297992.5A CN114106983A (en) | 2021-11-04 | 2021-11-04 | Microbe culture chip for spaceflight and preparation process thereof |
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| Publication Number | Publication Date |
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| CN114106983A true CN114106983A (en) | 2022-03-01 |
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| CN202111297992.5A Pending CN114106983A (en) | 2021-11-04 | 2021-11-04 | Microbe culture chip for spaceflight and preparation process thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006030465A (en) * | 2004-07-14 | 2006-02-02 | Taiei Denki Kk | Dish for living cell observation |
| CN202175680U (en) * | 2011-04-18 | 2012-03-28 | 北京航空航天大学 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
| US20120107913A1 (en) * | 2009-07-14 | 2012-05-03 | Dai Nippon Printing Co., Ltd. | Microorganism culture sheet and manufacturing method therefor |
| CN202430227U (en) * | 2011-12-20 | 2012-09-12 | 重庆大学 | Flat plate flowing chamber capable of implementing electric stimulation in multiple directions |
| JP2013215175A (en) * | 2012-04-12 | 2013-10-24 | Daiko Kennetsu Kk | Medium for hydroponics |
| CN104845888A (en) * | 2015-02-13 | 2015-08-19 | 中国航天员科研训练中心 | Totally-enclosed cell culture plate |
-
2021
- 2021-11-04 CN CN202111297992.5A patent/CN114106983A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006030465A (en) * | 2004-07-14 | 2006-02-02 | Taiei Denki Kk | Dish for living cell observation |
| US20120107913A1 (en) * | 2009-07-14 | 2012-05-03 | Dai Nippon Printing Co., Ltd. | Microorganism culture sheet and manufacturing method therefor |
| CN202175680U (en) * | 2011-04-18 | 2012-03-28 | 北京航空航天大学 | Coculture double-channel mechanical force loading flow chamber device for in vitro cells |
| CN202430227U (en) * | 2011-12-20 | 2012-09-12 | 重庆大学 | Flat plate flowing chamber capable of implementing electric stimulation in multiple directions |
| JP2013215175A (en) * | 2012-04-12 | 2013-10-24 | Daiko Kennetsu Kk | Medium for hydroponics |
| CN104845888A (en) * | 2015-02-13 | 2015-08-19 | 中国航天员科研训练中心 | Totally-enclosed cell culture plate |
Non-Patent Citations (1)
| Title |
|---|
| 邢焰 北京理工大学出版社: "航天器材料", pages: 254 - 255 * |
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