CN202430227U - Flat plate flowing chamber capable of implementing electric stimulation in multiple directions - Google Patents

Flat plate flowing chamber capable of implementing electric stimulation in multiple directions Download PDF

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Publication number
CN202430227U
CN202430227U CN2011205388392U CN201120538839U CN202430227U CN 202430227 U CN202430227 U CN 202430227U CN 2011205388392 U CN2011205388392 U CN 2011205388392U CN 201120538839 U CN201120538839 U CN 201120538839U CN 202430227 U CN202430227 U CN 202430227U
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plate
flow chamber
electrode
provided
flat plate
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CN2011205388392U
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Chinese (zh)
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吕永钢
邹杨
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重庆大学
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Abstract

The utility model discloses a flat plate flowing chamber capable of implementing electric stimulation in multiple directions. The flat plate flowing chamber is formed by overlapping three layers of flat plates and two layers of gaskets, wherein an upper flat plate (2) is provided with a fluid inlet/outlet; an upper gasket (3) is provided with a flow guiding groove (4); a middle flat plate (5) is provided with a rectangular slit (6); a lower gasket (10) is provided with a small flowing chamber (18); the middle flat plate (5) is provided with an electrode plate (7) above the small flowing chamber (18); a lower flat plate (11) is provided with an electrode plate (16) below the small flowing chamber (18); and a pair of electrodes (14 and 17) are respectively placed at four edges of the small flowing chamber (18). A shearing force is applied to cells (19) and the electric stimulation is carried out by loading current of different forms and strength on the different electrodes; and the device is simple and compact in structure and is convenient for follow-up observation of the cells under a microscope.

Description

可实施多方向电刺激的平板流动腔 Embodiments may be multi-directional electrical stimulation plate flow chamber

技术领域 FIELD

[0001] 本发明涉及ー种平板流动腔,特别是可施加多方向电刺激的平板流动腔。 [0001] The present invention relates to species ー plate flow chamber, in particular multi-directional electrical stimulation may be applied flat plate flow chamber.

背景技术 Background technique

[0002] 平板流动腔是体外研究切应カ对细胞生物学特性影响的主要装置之一,可用于研究细胞粘附、细胞聚集、细胞变性、细胞运动和细胞对力学刺激响应等诸多领域,其核心部分是ー个高度远小于长度和宽度的扁平流动腔室。 [0002] In vitro studies plate flow chamber is one of the main cutting apparatus grades should affect the biological characteristics of the cells can be used to study cell adhesion, cell aggregation, cell degeneration, cell motility and cellular response to mechanical stimuli and many other fields, which the core part is much smaller than the height of a flat ー flow chamber length and width. 平板流动腔设计中一般力求组装简単,可操作性強,同时便于实时观察。 Plate flow chamber assembly design generally sought radiolabeling simple, easy to operate, while facilitating real-time observation.

[0003] 多数平板流动腔里为定常层流。 [0003] Most plate flow chamber in a laminar flow is steady. 在此基础上,根据研究的需要已出现各式各样的平板流动腔。 Based on this, a variety of flat plate flow chamber studies have emerged as needed. 为研究流动剪切力作用下两细胞的相互作用,上海交通大学沈宝荣等提出一种由聚苯こ烯底座、双面胶和聚碳酸酯上板构成的细胞联合培养流动腔,所提供的流动腔可方便地进行实时显微镜观察,也有助于进行细胞电生理膜片钳技术研究(沈宝荣,严志強,齐颖新,许文燮,姜宗来,细胞联合培养流动腔,中国发明专利,申请号:201010216353. 7)。 Flow of shear force interaction of two cells, Shen Bao-rong Shanghai Jiaotong put forward a combined flow from the cell culture chamber flow alkenyl ko polystyrene base, and double-sided adhesive sheet made of polycarbonate, provided chamber may conveniently be carried out in real-time microscopic observation, but also facilitate the study of cell electrophysiological patch clamp technique (Shen Bao-rong, Yan Zhiqiang, Qi Ying new, Xu Wenxie, ginger cases, the cells were co-cultured flow chamber, Chinese invention patent application number: 2010102163537 ). 北京航空航天大学樊瑜波等提出ー种利用可滲透膜分隔成两个流动腔的细胞联合培养流动腔,同时实现两种细胞在线共培养的同时施加剪切应カ(樊瑜波,孙联文,杨肖,蒲放,贡向辉,用于体外细胞共培养的力学加载流动装置,中国发明专利,申请号:201010293367. 9)。 Beijing University of Aeronautics and Astronautics Fan Yubo put forward ー kinds of use of permeable membrane into two flow chambers CELL CULTURE flow chamber, while achieving simultaneous application of both cell-line co-cultured shear ka (Fan Yubo, Sun joint text, Yang Xiao, Pu put, Gong Xiang-Hui, in vitro cell co-culture for the mechanical loading of the mobile device, the Chinese invention patent application number: 2010102933679). 为使流动腔底面获得大范围层流剪切力分布均匀区域,四川大学刘肖珩等提出一种非对称入口平行平板流动腔,采用非対称的T型入流口和水平出流ロ结构,在入流ロ与出流ロ处各设有ー个高度大于出入流ロ直径的缓冲池(刘肖珩,曾烨,吴江,非対称入口平行平板流动腔,中国发明专利,申请号:201010165038. 6)。 For flow chamber bottom surface to obtain a large range of laminar shear stress distribution area, Sichuan University Liu Xiao Heng put forward an asymmetrical inlet of a parallel plate flow chamber, using T-type inlet port and the level of non Dui said outflow ro structure, the inflow ro and provided at the respective outflow ro ー a height greater than the flow out of the buffer pool ro diameter (Heng Xiao Liu, Ye had Wujiang, non Dui parallel plate flow chamber, said inlet, Chinese invention patent application number: 2010101650386). 为模拟内皮细胞在流动环境,上海理工大学丁皓等提出一种圆管流动腔内皮细胞培养装置,使细胞同时受到周向切应力、切应力、正应カ等(丁皓,赵灵犀,尚昆,陈齐欧,徐晶晶,沈カ行,圆管流动腔内皮细胞培养装置,中国发明专利,申请号:2010101519154. 3)。 Endothelial cells to simulate flow environment, Shanghai University Dinghao endothelial cells put forward flow chamber means one kind of pipe, while the cells by circumferential shear stress, shear stress, etc. is to be the ka (Ding Hao, Zhao consonance, Shang Kun Chen Qi Europe, Xu Jingjing, Shen ka row, endothelial cells pipe flow chamber means, Chinese invention patent application number: 20,101,015,191,543). 为模拟脉动流动,上海理工大学丁皓等提出ー种平板流动腔细胞培养系统(丁皓,尚昆,赵灵犀,沈カ行,虞倩倩,缪伟伟,吴芳芳,平板流动腔细胞培养系统,中国发明专利,申请号:201110088607. 6)。 For the simulation of turbulent flows, Shanghai University Ding Hao and other kinds of proposed ー plate flow chamber cell culture system (Ding Hao, Shang Kun, Zhao consonance, Shen ka row, Yu Qianqian, Miao Weiwei, Wu Fangfang, cell culture plate flow chamber system, Chinese invention patent , application number: 2011100886076). 为获得连续剪切力作用,中国科学院力学研究所钱民全等提出ー种体外细胞培养平行圆板流动腔,包括上下两块间距可调的同轴圆板,可形成从圆心沿半径方向到圆板边缘呈线性増加的剪切力作用(钱民全,彭荣蕤,赵笃凤,钱大兴,萧蓝,体外细胞培养平行圆板流动腔,中国实用新型专利,专利号:ZL 01231650. 4)。 To obtain a continuous shearing force, China Institute of Mechanics of money proposed ー congruent species cell culture in vitro parallel plate flow chamber circle, comprising two coaxial circular plates vertically adjustable pitch, may be formed from the center to the radial direction circular plate edge linearly to increase in shear force (money China full, PENG Rong Rui Zhao Benedict Feng, money Hing, Xiao blue, in vitro cell culture parallel circular plate flow chamber, Chinese utility Model Patent number: ZL 01231650. 4). 他们还提出ー种将从两块平板腔体入口到腔体出口方向为倾斜的腔体,实现连续剪切力作用(钱民全,彭荣蕤,赵笃凤,钱大兴,萧蓝,体外细胞培养装置中的非平行平板流动腔,中国实用新型专利,专利号:ZL 01231652.0)。 They also suggested ー species from the inlet of two plates of the cavity to the outlet direction of the cavity is inclined cavity, continuous shear stress (money China full, PENG Rong Rui Zhao Benedict Feng, money Hing, Xiao blue vitro cell unit cultured non-parallel plate flow chamber, Chinese utility Model Patent number: ZL 01231652.0). 然而,目前还少见有装置可以同时对细胞施加剪切カ和电刺激的流动腔装置,特别是能施加多个方向电刺激的平板流动腔。 However, there is still rare means may be a shearing grades and electrical stimulation of cell flow chamber device, in particular a plurality of directions can be applied to a flat plate flow chamber electrical stimulation.

发明内容 SUMMARY

[0004] 本发明要解决的技术问题是提供一种可同时对施加流体剪切カ和电刺激的平板流动腔,不仅可以施加多个方向电刺激,而且方便后续显微镜下观察细胞。 [0004] The present invention is to solve the technical problem is to provide a simultaneous application of fluid shear plate flow chamber and ka electrical stimulation, the plurality of directions can be applied not only electrical stimulation, but also facilitate subsequent observed under a microscope.

[0005] 为解决上述技术问题,本发明的可实施多方向电刺激的平板流动腔包括三层平板和两层垫片,上平板设置有流体进出ロ,上垫片设置有导流槽,中平板设置矩形狭縫,下垫片设置有流动小室。 [0005] In order to solve the above technical problems, embodiments of the present invention may be multi-directional electrical stimulation plate flow chamber comprises three layers and two spacer plates, the plate is provided with a fluid out of ro, the liner is provided with guide grooves, in a rectangular slit plate is provided, the liner is provided with a flow cell. 培养液由上平板流体进ロ进入上垫片中三角形导流槽,使得流体充分发散并受到缓冲的作用有效的降低入口的扰流现象。 On a plate by the fluid medium enters into the triangular guide groove ro gasket such that the fluid diverges and subjected to sufficient buffer action effectively reduce turbulence phenomena inlet. 充分发散的培养液由中平板上矩形狭缝进入流体动小室。 Central broth sufficiently diverging rectangular slit plate movable into the fluid chamber. 为保证流体流过流体动小室时满足层流、ニ维流动、充分发散的流动,流体动小室宽度和长度均应大大于流体动小室厚度与放在其中的电极板的厚度之差。 To ensure laminar flow of fluid through the fluid moving satisfied cells, Ni-dimensional flow, the flow sufficiently divergent, hydrodynamic chamber width and length shall be substantially movable in the fluid chamber on the thickness and difference in thickness of the electrode plates therein. 为进一步实现多方向的电刺激,所述中平板在位于流动小室上方设有电极板,下平板在位于流动小室下方放置电极板,流动小室四边分别放置一对电极和电极。 Electrical stimulation is further achieved in multiple directions, in the flat plate is provided above the electrode flow chamber, located below the lower plate in an electrode plate flow chamber disposed, four sides of the flow chamber and the electrode is placed a pair of electrodes, respectively.

[0006] 为方便后续显微镜下观察细胞,下平板上放置的电极板由铟锡氧化物导电玻璃制得,可以加载电刺激又透明。 [0006] To facilitate the subsequent cells were observed, the electrode plate is placed on the lower plate is made of indium tin oxide conductive glass, and a transparent electrical stimulation can be loaded. 此电极可以制备成厚度一致的平板,实现均一的流体剪切力作用;也可以制备成厚度均匀变化的楔形板,实现连续剪切力作用。 The electrode can be prepared in a uniform thickness plate, the fluid shear force to realize a uniform; can also be prepared into a uniform thickness varying wedge plate, continuous shear force. 在进行流动腔实验时在电极表面种细胞,或者根据实验的需要对电极表面进行表衬再种细胞。 Types of cells on the electrode surface, or the surface of the electrode required for the experiment during the experiment flow chamber substrate table further types of cells. 中平板上电极板通过胶粘在中平板上,其连接导线通过刻在中平板上的小槽引出,利用密封胶密封。 The upper plate of the electrode plate in the plate, which is connected by a wire by gluing plate engraved on the small grooves drawn by sealant. 中平板上电极板和流动小室四周电极板均可由铟锡氧化物导电玻璃或者铟锡氧化物导电膜或者银、金或钼等贵金属板制得。 The upper plate and the plate electrode flow chamber may have four weeks electrode plate conductive glass made of indium tin oxide or indium tin oxide conductive film, or silver, gold or other precious metal plate made of molybdenum. 连接这些电极板的导线铺设在下垫片上刻的小槽,放置好导线后用密封胶密封。 The connecting wire electrode plate laid on the lower spacer small engraved grooves, the wire in place sealed with sealant.

[0007] 平板流动腔通过螺栓或者夹具装配好后,通过聚四氟こ烯导管与储液槽和蠕动泵构成ー个的循环流动的体系。 [0007] After plate flow chamber assembly by the bolts or clamps, through conduit polytetrafluoroethylene ko alkenyl peristaltic pump and reservoir constitute a system of ー circulating. 储液槽中的液体由蠕动泵泵出后,沿着导管流入平板流动腔,对电极板上的细胞施加剪切力作用,然后流出,沿着流出导管回到储液槽中。 After the liquid reservoir is pumped by a peristaltic pump, flows along the duct plate flow chamber, a shear force effect on the cell electrode plate, and then flows back to the reservoir along an outflow conduit. 工作时,除蠕动泵以外,其他装置都放在培养箱内。 In operation, in addition to peristaltic pumps, other devices are placed in the incubator. 通过信号发生器和稳压电源给各对电极施加连续、脉冲等多种作用形式和強度的电刺激。 Applying electrical stimulation and strength of various forms of continuous action, and the like by the pulse signal generator and power supply to each pair of electrodes. 电刺激时可以単独一对电极作用,也可以几对电极同时作用。 Only a pair of electrodes can radiolabeling role when electrical stimulation, can also act simultaneously several pairs of electrodes.

[0008] 综上所述,本发明的平板流动腔通过在流动小室六个面安装电极板,实现对细胞施加剪切力的同时,在不同电极上加载不同形式和强度电流对细胞进行电刺激。 [0008] In summary, the present invention is plate flow chamber in the flow cell by six surface-mounted electrode plate, while applying a shearing force to achieve the cells, and intensity of the current loading different forms in different electrode cells are electrically stimulated . 与现有平板流动腔相比,利用透明铟锡氧化物导电玻璃制备放置在流动小室底部的电极有利于后续显微镜下观察细胞,还可以通过改变透明铟锡氧化物导电玻璃厚度实现均一流体剪切力或连续剪切力作用,这也是本发明提出的关键之一。 Compared with the conventional flat plate flow chamber, with a transparent conductive indium tin oxide electrode prepared glass was placed at the bottom of the flow chamber facilitates later cells were observed, the shear fluid may also be implemented by changing the uniform transparent indium tin oxide conductive glass thickness continuous force or shear forces, which is one of the key according to the present invention is proposed. 本发明提供的平板流动腔可根据实验要求选择不同的电刺激方式和強度,也可以选择不同的流体剪切力作用,所实现的平板流动腔结构简单紧凑,有望在更多生物力学研究中发挥重要作用。 Plate flow chamber in accordance with the present invention may be provided in different experimental requirements and mode select electrical stimulation intensity, selection of a different fluid shear force, plate flow chamber to achieve a simple and compact structure, it is expected to play in more Biomechanical Research an important role. 用于制备放置在流动小室底部的电极的材料不仅局限于铟锡氧化物导电玻璃,只要选择的材料透明且导电,对细胞无明显毒性即可。 Materials used to make the electrodes placed at the bottom of the flow cell is not limited to indium tin oxide conductive glass, as long as the transparent and conductive material selected, no significant toxicity to cells. 为简洁起见,本发明主要以铟锡氧化物导电玻璃为例加以阐述,其他透明导电材料也可以采用相同的原理。 For brevity, the present invention is primarily indium tin oxide conductive glass to be set forth as an example, other transparent conductive material, the same principle can also be employed.

附图说明 BRIEF DESCRIPTION

[0009] 图I是本发明提供的可实施多方向电刺激的平板流动腔的结构示意图; [0009] FIG. I is a schematic structural diagram of the present invention to provide a multi-directional plate flow chamber electrical stimulation may be implemented;

[0010] 图2是本发明提供的可实施多方向电刺激的平板流动腔的剖视图; [0010] FIG. 2 is a cross-sectional view of a flat plate flow chamber may be multi-directional embodiment of the present invention, electrical stimulation is provided;

[0011] 图3是本发明提供的可实施多方向电刺激的平板流动腔的俯视图; [0011] FIG. 3 is a top plan view of a plate flow chamber may be multi-directional embodiment of the present invention, electrical stimulation is provided;

[0012] 图4是本发明提供的可实施多方向电刺激的平板流动腔的仰视图;[0013] 图5是图2所示可实施多方向电刺激的平板流动腔沿图2中的AA线的剖视图; [0012] FIG. 4 is a bottom view of a plate flow chamber may be multi-directional embodiment of the present invention, electrical stimulation is provided; [0013] FIG. 5 is a view of AA in embodiment 2 of the multi-directional electrical stimulation plate flow chamber 2 shown in FIG. cross-sectional view of the line;

[0014] 图6是图2所示可实施多方向电刺激的平板流动腔沿图2中的BB线的剖视图; [0014] FIG. 6 is shown in FIG embodiment more than two directions may be electrically stimulating plate flow chamber cross-sectional view along line 2 BB;

[0015] 图7是图2所示可实施多方向电刺激的平板流动腔沿图2中的CC线的剖视图; [0015] FIG. 7 is shown in FIG embodiment more than two directions may be electrically stimulating plate flow chamber cross-sectional view along line 2 CC;

[0016] 图8是本发明提供的可实施多方向电刺激的平板流动腔的另ー实施例中流动小室底面电极板的剖视图。 [0016] FIG. 8 is an embodiment of the present invention can provide a multi-directional electrical stimulation ー plate flow chamber cross-sectional view of another embodiment of the flow cell embodiment the bottom electrode plate.

[0017] 其中:1_导管;2_上平板;3_上垫片;4_导流槽;5_中平板;6_矩形狭缝;7_电极板;8_导线;9_导线;10_下垫片;11_下平板;12_导线;13_导线;14_电极;15_导线;16-电极板;17_电极;18-流动小室;19_细胞;20_螺检;21_螺帽。 [0017] wherein: 1_ conduit; 2_ upper plate; 3_ upper pad; 4_ guide groove; 5_ the plate; 6_ a rectangular slit; 7_ electrode plate; 8_ wire; 9_ wire; 10_ lower pad; tablet 11_; 12_ wire; 13_ wire; 14_ electrode; 15_ lead; 16- electrode plate; 17_ electrode; flow chamber 18; 19_ cells; 20_ spiro subject; 21_ nut.

具体实施方式 Detailed ways

[0018] 以下实施例用于说明本发明,但不用来限制本发明的范围。 [0018] The following examples serve to illustrate the present invention but are not intended to limit the scope of the present invention.

[0019] 图I是本发明提供的可实施多方向电刺激的平板流动腔的结构示意图,其结构剖视图、俯视图和仰视图分别如图2、图3和图4所示,图5、图6和图7分别是图2中AA线、BB线和CC线的剖视图,图8是本发明提供的可实施多方向电刺激的平板流动腔的另ー实施例中流动小室底面电极板的剖视图。 [0019] FIG. I is a schematic structural plate flow chamber of the present invention is provided may be embodied multi-directional electrical stimulation, the structure of a cross-sectional view, a top view and a bottom view, respectively, FIGS. 2, 3 and 4, FIG. 54, FIG. 6 and FIG. 7 respectively in FIG line AA 2, a cross-sectional view along the line BB and line CC, FIG. 8 is provided by the invention may be embodied multi-directional electrical stimulation plate flow chamber further ー sectional view of the flow chamber bottom electrode plate embodiment. 由图I、图2、图3和图4可知,本发明的可实施多方向电刺激的平板流动腔由三层平板和两层垫片叠加组成,上平板2设置有流体进出ロ,上垫片3设置有导流槽4,中平板5设置矩形狭缝6,下垫片10设置有流动小室18。 , 2, 3 and 4 can be seen from Figure I, the electrical stimulation may be multi-directional embodiment of the present invention consists of three flat-plate flow chamber and composed of two superimposed gasket, the plate 2 is provided with a fluid out of ro, the pad sheet 3 is provided with guide grooves 4, 5 is provided with a rectangular slit in plate 6, the gasket 10 is provided with a flow chamber 18. 流体动小室18宽度和长度均应大大于流体动小室18厚度与放在其中的电极板16的厚度之差。 Hydrodynamic chamber 18 should be substantially the width and length of the fluid chamber 18 and movable on the thickness difference between the thickness of the electrode plate 16 therein. 上平板2、中平板5和下平板11可由聚碳酸酷、树脂玻璃或金属等材料制备,上垫片3和下垫片10可由硅树脂等制备。 Upper plate 2, the plate 5 and the lower plate 11 may cool polycarbonate, glass or metal was prepared resin material, the spacer 3 and the lower gasket 10 may be a silicone resin was prepared. 如图5、图6和图7可知,培养液由上平板2上导管I进入上垫片3中三角形导流槽4,使得流体充分发散并受到缓冲的作用有效的降低入口的扰流现象。 5, 6 and 7, the culture broth by the upper plate 2 I conduit 3 into the upper triangle washer guide groove 4, so that the fluid diverges and subjected to sufficient buffer action effectively reduce turbulence phenomena inlet. 充分发散的培养液由中平板5上矩形狭缝6进入流体动小室18。 Central sufficiently divergent broth plates 5 on a rectangular slit 6 enters hydrodynamic chamber 18.

[0020] 所述中平板5在位于流动小室18上方设有电极板7。 [0020] The plate 5 are positioned above the flow chamber 18 is provided with electrode plate 7. 由铟锡氧化物导电玻璃制成的电极(16放置在下平板11上,位于流动小室18下方。流动小室18四边分别放置ー对电极14和电极17。电极16可以制备成厚度一致的平板,实现均一的流体剪切力作用。由图8可知,电极16也可以制备成厚度均匀变化的楔形板,实现连续剪切力作用。在进行流动腔实验时在电极16表面种细胞19,或者根据实验的需要对电极16表面进行表衬再种细胞19。电极板7、电极14和电极17由铟锡氧化物导电玻璃或者铟锡氧化物导电膜或者银、金或钼等贵金属板制得。中平板5上电极板7通过胶粘连接,电极板7的连接导线通过刻在中平板5上的小槽引出,并通过密封胶密封。下垫片10上刻有小槽,用于铺设连接电极板16、电极14和电极17的导线8、导线13和导线15、导线9和导线12,导线放入小槽后再用密封胶密封。 Electrode made of indium tin oxide conductive glass (16 is placed on the lower plate 11, located below the flow cell 18 flow chamber 18 disposed respectively at the four ー electrode 14 and the counter electrode 17. The electrode 16 may be prepared as a plate of uniform thickness, to achieve a uniform fluid shear force seen from Figure 8, the electrodes 16 may also be prepared uniformly varying thickness wedge plate, continuous shear force. when carrying experiments flow chamber surface 16 kinds of electrode cells 19, or according to experiments the surface electrode 16 is required for re-lining table 19. the electrode plate 7 types of cells, the electrode 14 and the electrode 17 is made of conductive glass of indium tin oxide or indium tin oxide conductive film, or silver, gold or other precious metal plate made of molybdenum. in upper electrode plate 5 through 7 plate engraved on the plate 5 by connecting a small groove lead wire glued connection, the electrode plate 7 and lower spacer sealant engraved by the small groove 10, the connection electrode for laying plate 16, the lead electrode 14 and the electrode 17 8, 13 and the lead wires 15, lead wires 9 and 12, then the groove leads into a small sealed with sealant.

[0021] 上平板2、中平板5、下平板11、上垫片3和下垫片10同一位置处加工有通孔,用于装配螺栓20,通过螺帽21压紧装配。 [0021] The upper plate 2, the plate 5, the lower plate 11, the spacer 3 at the same position of the workpiece and the pad has a through hole 10 for mounting bolt 20, the nut 21 by pressing the assembly. 螺栓20的个数可以根据平板流动腔的大小对称设置4-40个。 The number of bolts 20 may be symmetrically arranged according to the size of 4-40 plate flow chamber. 在平板流动腔尺寸较小时也可以直接用夹具压紧装配,不设置螺栓20连接。 When small plate flow chamber dimensions may be assembled directly with the pressing jig, connecting bolt 20 is not provided. 使用吋,将接种好细胞19的电极板16放入流动小室18后,通过螺栓或者夹具装配好平板流动腔,通过聚四氟こ烯导管与储液槽和蠕动泵构成一个的循环流动的体系。 Use inch, seeded 19 well cell plate electrode 16 is placed in the 18 by bolts or clamps assembled plate flow chamber flow cell, constituting the circulating system, through a conduit and polytetrafluoroethylene ko alkenyl peristaltic pump and reservoir . 储液槽中的液体由蠕动泵泵出后,沿着导管流入平板流动腔,对电极板16上的细胞19施加剪切力作用,然后流出,沿着流出导管回到储液槽中。 After the liquid reservoir is pumped by a peristaltic pump, flows along the duct plate flow chamber, a shearing force is applied to the cells on the electrode plate 1619, and then flows back to the reservoir along an outflow conduit. 工作时,除蠕动泵以外,其他装置都放在培养箱内。 In operation, in addition to peristaltic pumps, other devices are placed in the incubator. 通过信号发生器和稳压电源给各对电极施加连续、脉冲等多种作用形式和強度的电刺激。 Applying electrical stimulation and strength of various forms of continuous action, and the like by the pulse signal generator and power supply to each pair of electrodes. 电刺激时可以単独ー对电极作用,也可以几对电极同时作用。 Can only ー radiolabeling of electrode effect can be several pairs of electrodes at the same time when the role of electrical stimulation.

[0022] 本发明提供的平板流动腔通过在流动小室六个面安装电极板,实现对细胞施加剪切力的同时,在不同电极上加载不同形式和强度电流对细胞进行电刺激。 [0022] The present invention provides a plate flow chamber in the flow cell by six surface-mounted electrode plate, while applying a shearing force to achieve the cells, and intensity of the current loading different forms in different electrode cells are electrically stimulated. 利用透明铟锡氧化物导电玻璃制备放置在流动小室底部的电极有利于后续显微镜下观察细胞,还可以通过改变透明铟锡氧化物导电玻璃厚度实现均一流体剪切力和连续剪切力作用。 Preparation of glass with a transparent conductive indium tin oxide is placed in the bottom of the flow chamber the electrodes facilitate subsequent observed under a microscope, can also achieve a uniform and continuous fluid shear forces by changing the shearing force indium tin oxide transparent conductive glass thickness. 正是由于这些综合因素,使得本发明提供的流动腔结构紧凑,可靠,相比以往的平板流动腔有很大的优势。 It is these combination of factors, such that the flow chamber structure of the present invention provides a compact, reliable, conventional plate flow chamber have great advantages compared.

Claims (5)

1. 一种可实施多方向电刺激的平板流动腔,由三层平板和两层垫片叠加组成,上平板设置有流体进出ロ,上垫片设置有导流槽,中平板设置矩形狭縫,下垫片设置有流动小室,其特征在于:所述中平板(5)在位于流动小室(18)上方设有电极板(7),由铟锡氧化物导电玻璃制成的电极板(16)放置在下平板(11)上、位于流动小室(18)下方,流动小室(18)四边分别放置一对电极(14)和电极(17)。 1. A multi-directional embodiment of electrical stimulation plate flow chamber, and two layers of a three-layer flat superposition of the spacer, the plate is provided with a fluid out of ro, the spacer is provided with a guide groove, a rectangular slit is provided in the plate , the liner is provided with a flow chamber, wherein: the said plates (5) provided with an electrode plate (7) located above the flow chamber (18), the electrode plate is made of indium tin oxide conductive glass (16 ) placed on the lower plate (11), located in the flow chamber (18) below the flow chamber (18), four sides placed a pair of electrodes (14) and an electrode (17), respectively.
2.根据权利要求I所述的可实施多方向电刺激的平板流动腔,其特征在于:所述电极板(16)为厚度一致的平板或厚度均匀变化的楔形板。 According to claim I of the embodiments may be multi-directional electrical stimulation plate flow chamber, wherein: said electrode plate (16) is uniformly varying thickness uniform thickness plate or wedge plate.
3.根据权利要求I所述的可实施多方向电刺激的平板流动腔,其特征在于:所述电极板(7)、电极(14)和电极(17)由铟锡氧化物导电玻璃或者铟锡氧化物导电膜或者银、金或钼贵金属板制得。 I according to the embodiments may be multi-directional electrical stimulation plate flow chamber, as claimed in claim wherein: said electrode plate (7), the electrodes (14) and an electrode (17) of indium tin oxide conductive glass or indium a conductive tin oxide film, or silver, gold or a noble metal sheet made of molybdenum.
4.根据权利要求I所述的可实施多方向电刺激的平板流动腔,其特征在于:所述下垫片(10)上刻有小槽,用于铺设连接电极板(16)、电极(14)和电极(17)的导线(8)、导线(13)和导线(15)、导线(9)和导线(12),上述导线放入小槽后用密封胶密封。 According to claim I of the embodiments may be multi-directional electrical stimulation plate flow chamber, wherein: said lower engraved on the spacer (10) small slots for laying connecting electrode plate (16), electrodes ( conductor (8) 14) and the electrode (17), the wire (13) and a wire (15), the wire (9) and the wire (12), sealed with sealant after said conductor placed in a small tank.
5.根据权利要求I所述的可实施多方向电刺激的平板流动腔,其特征在于:所述电极板(7)通过胶粘在中平板(5)上,电极板(7)的连接导线通过刻在中平板(5)上的小槽引出并通过密封胶密封。 I according to the embodiments may be multi-directional electrical stimulation plate flow chamber as claimed in claim, wherein: said electrode plate (7) is connected by gluing on the lead plates (5), the electrode plate (7) by engraved on the plate (5) is drawn through a small slot sealant.
CN2011205388392U 2011-12-20 2011-12-20 Flat plate flowing chamber capable of implementing electric stimulation in multiple directions CN202430227U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154315A (en) * 2015-09-30 2015-12-16 重庆大学 Flowing chamber for biofluid experiment
CN105176813A (en) * 2015-09-30 2015-12-23 重庆大学 Using method of flow chamber for bio-fluid experiment

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105154315A (en) * 2015-09-30 2015-12-16 重庆大学 Flowing chamber for biofluid experiment
CN105176813A (en) * 2015-09-30 2015-12-23 重庆大学 Using method of flow chamber for bio-fluid experiment
CN105154315B (en) * 2015-09-30 2017-12-01 重庆大学 A kind of biofluid experiment flow cavity

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